sp|P38661|PDIA6_MEDSA ----> Probable protein disulfide-isomerase A6

Intron 1 GTCTTTGTTA ... TGGCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.08	484	65.3	28
A2	11.23	388	53.1	19.1
A3	12.54	396	63.7	16.7
A4	10.83	454	60.9	28.1
A5	13.62	216	37.1	12.1
A6	10.53	965	125.3	40
A7	10.78	1177	157	52.7
A8	10.08	858	105.7	44.1
A9	11.18	1167	162.3	64.3
A10	11.29	952	133.7	48.7

>TRINITY_DN116719_c6_g1_i3 from sample A1 / Example spliced isoform

TTATTCTACGACGACCACATCTCGGGAGAGATCTGTATAAAGAGGAGACCAAGAAGAGGAGCGTGGATCATTTTTAGAGAGCCTGGTTTGTCGGCAGGCCGAGATTTAGTGGAGTTATAGTTCTAGGGTTTTAAAGGCCCCATTGATTTTGATCACCGTGAATCAAATTCTACGGTTTACAATGGGCAGAGGGTGGATCT
CGGCCGTGTCTGTGTTCGCATTTCTGCTCGTGGCGGTTCGATCTGATGACGTGACTGTCCTTACCCCCGACAATTTCGAGAATGAAGTAGGAAAAGACAGGGGCGCTCTCGTTGAATTTTACGCACCATGGTGTGGGCATTGTAAAAAACTCGCACCTGAATATGAGAAGCTTGGGGCAAGTTTCAAGAAGGTGAAATCT
GCGTTGATAGCAAAGGTTGACTGTGATGAGCATAAAAGCCTTTGCAGTAAGTATGGTGTTTCTGGTTTTCCAACTCTTAAGTGGTTCCCTAAAGGATCACTAGAACCAAAAGACTATAATGGTGGTCGCACTGCAGAAGACCTTACCACCTTTGTGAATACTGAAGCAGGAACTCATGTGAAATTGACTGTTCCTCAATC
TGCGGTAGTGGTGTTGACATCAGAAAACTTTGATTCTGTTGTTCTTGATGAATCAAAAGATGTTCTTGTTGAGTTCTATGCTCCATGGTGTGGTCACTGCAAAAATCTGGCCCCTACATATGAAAAGTTTGCAACAGCATTCAAATTAGAGAAAGATGTTGTGATTGCCAATGTTGATGCTGATAAATACAAGGACCTCG
GAGAAAAGTATGGAGTTAGTGGATTTCCAACTTTAAAGTTCTTTCCAAAGACCAATAAGGCTGGTGAAGATTATGATGGTGGTCGTGATTTGGATGCCTTTGTTACTTTTATCAATGAAAAAGCTGGCACAAACCGTGATGGGCAGGGCCGTCTGACATCCCTTGCTGGAAAAGTTGATAGCATGGATCATTTGGTGCAA
GAATTTTTGAGTGCTGGTGCTGATGAACGTGAGGCTATCTTGGCCAAATTCGAGTCAGAGGCTGAAAGGTTGTCTGGCCCTCATGCAAGCTATGGGAAGGTTTATCTGAAGACTGCCAAAAAGATAACTGAGAAGGGTGCAGACTATGCAAAGAACGAGGTTGAGCGACTGCAGCGTATCCTTGAAAAGGCTGTCAGTAC
CGCAAAAGCAGATGAGTTCACTGTGAAGAAAAACATCCTCTCATCCTTCTTAGCACAGTGATGGGATTTTTAAGTGCGCACAGACTTCAAAGTTCTAGAACATTTTAGTTGATTTAACTGTCTTGTATGGAAATATAATTGCTTAGCATTTGAAAAATGATTTCATAGCACGGTCAAACTAGAAGAGTCATGAGCCTTTT
GAGCAATTTAGCAATTTTATGGCGTTTAAAGCCATAGAGGTTACTCTCTGTAATTCGGGGGTGCAGTGAAACATATAATCTTTGTTTGATGCATGTGACTGGTACCCTTAAATATTTTCTTTCTATTAGTCTTTGTAGCATCAGTGAATGCATCCAATGATTGCCAATTGCCTTAAAAATGAGAAGTAGAGGCTCACCAA
TATTCAAACATTTAGTTTAACTTGCCTGAACAATGGATAGCATAATCCTGTGACCATATTGTAGGCCAAATTATCCTATTTGATGAGAAGACGCT

>TRINITY_DN116719_c6_g1_i4 from sample A1 / Example unspliced isoform:

TTATTCTACGACGACCACATCTCGGGAGAGATCTGTATAAAGAGGAGACCAAGAAGAGGAGCGTGGATCATTTTTAGAGAGCCTGGTTTGTCGGCAGGCCGAGATTTAGTGGAGTTATAGTTCTAGGGTTTTAAAGGCCCCATTGATTTTGATCACCGTGAATCAAATTCTACGGTTTACAATGGGCAGAGGGTGGATCT
CGGCCGTGTCTGTGTTCGCATTTCTGCTCGTGGCGGTTCGATCTGATGACGTGACTGTCCTTACCCCCGACAATTTCGAGAATGAAGTAGGAAAAGACAGGGGCGCTCTCGTTGAATTTTACGCACCATGGTGTGGGCATTGTAAAAAACTCGCACCTGAATATGAGAAGCTTGGGGCAAGTTTCAAGAAGGTGAAATCT
GCGTTGATAGCAAAGTTTTGCAAGGTTGACTGTGATGAGCATAAAAGCCTTTGCAGTAAGTATGGTGTTTCTGGTTTTCCAACTCTTAAGTGGTTCCCTAAAGGATCACTAGAACCAAAAGAgtctttgttataagtggctattgcctattagttgcatccatgaagtaaataaataaataatcgattaacattctggtt
tctgcttcttctttggctttaccttttattacttttaaaaattactcgacatgtgactttggttattggcttgcagCTATAATGGTGGTCGCACTGCAGAAGACCTTACCACCTTTGTGAATACTGAAGCAGGAACTCATGTGAAATTGACTGTTCCTCAATCTGCGGTAGTGGTGTTGACATCAGAAAACTTTGATTCT
GTTGTTCTTGATGAATCAAAAGATGTTCTTGTTGAGTTCTATGCTCCATGGTGTGGTCACTGCAAAAATCTGGCCCCTACATATGAAAAGTTTGCAACAGCATTCAAATTAGAGAAAGATGTTGTGATTGCCAATGTTGATGCTGATAAATACAAGGACCTCGGAGAAAAGTATGGAGTTAGTGGATTTCCAACTTTAAA
GTTCTTTCCAAAGACCAATAAGGCTGGTGAAGATTATGATGGTGGTCGTGATTTGGATGCCTTTGTTACTTTTATCAATGAAAAAGCTGGCACAAACCGTGATGGGCAGGGCCGTCTGACATCCCTTGCTGGAAAAGTTGATAGCATGGATCATTTGGTGCAAGAATTTTTGAGTGCTGGTGCTGATGAACGTGAGGCTA
TCTTGGCCAAATTCGAGTCAGAGGCTGAAAGGTTGTCTGGCCCTCATGCAAGCTATGGGAAGGTTTATCTGAAGACTGCCAAAAAGATAACTGAGAAGGGTGCAGACTATGCAAAGAACGAGGTTGAGCGACTGCAGCGTATCCTTGAAAAGGCTGTCAGTACCGCAAAAGCAGATGAGTTCACTGTGAAGAAAAACATC
CTCTCATCCTTCTTAGCACAGTGATGGGATTTTTAAGTGCGCACAGACTTCAAAGTTCTAGAACATTTTAGTTGATTTAACTGTCTTGTATGGAAATATAATTGCTTAGCATTTGAAAAATGATTTCATAGCACGGTCAAACTAGAAGAGTCATGAGCCTTTTGAGCAATTTAGCAATTTTATGGCGTTTAAAGCCATAG
AGGTTACTCTCTGTAATTCGGGGGTGCAGTGAAACATATAATCTTTGTTTGATGCATGTGACTGGTACCCTTAAATATTTTCTTTCTATTAGTCTTTGTAGCATCAGTGAATGCATCCAATGATTGCCAATTGCCTTAAAAATGAGAAGTAGAGGCTCACCAATATTCAAACATTTAGTTTAACTTGCCTGAACAATGGA
TAGCATAATCCTGTGACCATATTGTAGGCCAAATTATCCTATTTGATGAGAAGACGCT

Intron 2 GTGAATTACA ... TTTGTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.87	447	66	5
A2	12.9	351	52	6
A3	19.69	420	103	6
A4	15.67	382	71	10
A5	17.24	240	50	1
A6	16.82	1177	238	9
A7	17.99	1422	312	8
A8	16.11	885	170	10
A9	16.36	1212	237	9
A10	16.93	1060	216	11

>TRINITY_DN98320_c0_g1_i3 from sample A4 / Example spliced isoform

CGACCACATCTCGGGAGAGATCTGTATAAAGAGGAGACCAAGAAGAGGAGCGTGGATCATTTTTAGAGAGCCTGGTTTGTCGGCAGGCCGAGATTTAGTGGAGTTATAGTTCTAGGGTTTTAAAGGCCCCATTGATTTTGATCACCGTGAATCAAATTCTACGGTTTACAATGGGCAGAGGGTGGATCTCGGCCGTGTCT
GTGTTCGCATTTCTGCTCGTGGCGGTTCGATCTGATGACGTGACTGTCCTTACCCCCGACAATTTCGAGAATGAAGTAGGAAAAGACAGGGGCGCTCTCGTTGAATTTTACGCACCATGGTGTGGGCATTGTAAAAAACTCGCACCTGAATATGAGAAGCTTGGGGCAAGTTTCAAGAAGGTGAAATCTGCGTTGATAGC
AAAGGTTGACTGTGATGAGCATAAAAGCCTTTGCAGTAAGTATGGTGTTTCTGGTTTTCCAACTCTTAAGTGGTTCCCTAAAGGATCACTAGAACCAAAAGACTATAATGGTGGTCGCACTGCAGAAGACCTTACCACCTTTGTGAATACTGAAGCAGGAACTCATGTGAAATTGACTGTTCCTCAATCTGCGGTAGTGG
TGTTGACATCAGAAAACTTTGATTCTGTTGTTCTTGATGAATCAAAAGATGTTCTTGTTGAGTTCTATGCTCCATGGTGTGGTCACTGCAAAAATCTGGCCCCTACATATGAAAAGTTTGCAACAGCATTCAAATTAGAGAAAGATGTTGTGATTGCCAATGTTGATGCTGATAAATACAAGGACCTCGGAGAAAAGTAT
GGAGTTAGTGGATTTCCAACTTTAAAGTTCTTTCCAAAGACCAATAAGGCTGGTGAAGATTATGATGGTGGTCGTGATTTGGATGCCTTTGTTACTTTTATCAATGAAAAAGCTGGCACAAACCGTGATGGGCAGGGCCGTCTGACATCCCTTGCTGGAAAAGTTGATAGCATGGATCATTTGGTGCAAGAATTTTTGAG
TGCTGGTGCTGATGAACGTGAGGCTATCTTGGCCAAATTCGAGTCAGAGGCTGAAAGGTTGTCTGGCCCTCATGCAAGCTATGGGAAGGTTTATCTGAAGACTGCCAAAAAGATAACTGAGAAGGGTGCAGACTATGCAAAGAACGAGGTTGAGCGACTGCAGCGTATCCTTGAAAAGGCTGTCAGTACCGCAAAAGCAG
ATGAGTTCACTGTGAAGAAAAACATCCTCTCATCCTTCTTAGCACAGTGATGGGATTTTTAAGTGCGCACAGACTTCAAAGTTCTAGAACATTTTAGTTGATTTAACTGTCTTGTATGGAAATATAATTGCTTAGCATTTGAAAAATGATTTCATAGCACGGTCAAACTAGAAGAGTCATGAGCCTTTTGAGCAATTTAG
CAATTTTATGGCGTTTAAAGCCATAGAGGTTACTCTCTGTAATTCGGGGGTGCAGTGAAACATATAATCTTTGTTTGATGCATGTGACTGGTACCCTTAAATATTTTCTTTCTATTAGTCTTTGTAGCATCAGTGAATGCATCCAATGATTGCCAATTGCCTTAAAAATGAGAAGTAGAGGCTCACCAATATTCAAACAT
TTAGTTTAACTTGCCTGAACAATGGATAGCATAATCCTGTGACCATATTGTAGGCCAAATTATCCTATTTGATGAGAAGATGCTATAGATCTCTTTTATTTAAA

>TRINITY_DN98320_c0_g1_i2 from sample A4 / Example unspliced isoform:

CGACCACATCTCGGGAGAGATCTGTATAAAGAGGAGACCAAGAAGAGGAGCGTGGATCATTTTTAGAGAGCCTGGTTTGTCGGCAGGCCGAGATTTAGTGGAGTTATAGTTCTAGGGTTTTAAAGGCCCCATTGATTTTGATCACCGTGAATCAAATTCTACGGTTTACAATGGGCAGAGGGTGGATCTCGGCCGTGTCT
GTGTTCGCATTTCTGCTCGTGGCGGTTCGATCTGATGACGTGACTGTCCTTACCCCCGACAATTTCGAGAATGAAGTAGGAAAAGACAGGGGCGCTCTCGTTGAATTTTACGCACCATGGTGTGGGCATTGTAAAAAACTCGCACCTGAATATGAGAAGCTTGGGGCAAGTTTCAAGAAGGTGAAATCTGCGTTGATAGC
AAAGgtgaattacaccctctttaaatatcttttatattgatagagttatatttggattcagtatatgcatgtttttttccagttgtcagtttaagattatttttcttctttatcttccacatattttgttgtttgaagttttgcaaggtacattcttgaatctgttgttgggtggtagtcgtacaagacatctgtgcgaa
acataatttgaaatttgttcaggggctgcatttccctttacgaatccatatcatggtttatttgatatttaatttcaagatgtgcatgtgctttcctgtcttgatctgcaagtgaaaatttgaccaagtattgagtaaagccagagttgcaattcgtgacaattagccttgtaaagattcagtgcattaccctgtatact
ggttgtgaatactgacatgaatattcaccaacaattccatttttcatgcattacccagtgacaagtttttaatattttaacctcgcggggcacgttgcctgactgatctttttgttacagGTTGACTGTGATGAGCATAAAAGCCTTTGCAGTAAGTATGGTGTTTCTGGTTTTCCAACTCTTAAGTGGTTCCCTAAAGG
ATCACTAGAACCAAAAGA