NA ----> NA

Intron 1 GTACATTTCT ... TTCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.51	24.3	75	16
A2	73.74	26	73	19
A3	84.71	13	72	10
A4	75.76	8	25	17
A5	92.5	3	37	10
A6	83.98	106.7	559	144
A7	85.13	86.7	496	112
A8	86.55	124	798	173
A9	86.53	104	668	168
A10	80.99	65	277	88

>TRINITY_DN110770_c0_g1_i1 from sample A2 / Example spliced isoform

CGAATCTCAAGCGAAGCCGGCCGGTACTTAATATTTATTGAATAAAGAGGCCCGAGGGTTTCAATCGACGGTCATCTCTGTAAATAAATGATCCTCCGTCGTTACTGTAGCGAAGGCCGTCCTAATGGCAGGGAAGCGAAAGAATGAGACATGGCATTCAACAAGTATCCAATATAGTGAACTTCTTCAAGGACTTCTTT
GACAGGAGTGGTTGTCAAGCTGAACTTCTTAAGGACGTGCTTACATTTGTTATTGTTACAGAACACTGCAAGAAATTAAGATTTCTGTCCTCTGTTTCCTGTGCTTGCATGGGGTCGGTATTATACAGTGACTATAAGATTATGATATCAGGATATTGGATTTAATATTTTTTCTCCACTGCAATACCATGAGATAATAA
TGGTTAAATTTTAGTTTTTGGTGCACAAATTGAGTCACTTACTGGCATTTCATCATTTGTTCACTATGGTCTATGTCTTATAAAATTGGTTATCTGAGCAGTAAATCACCAGATATTAAGCTCCATTTGTTTTTCATTCTCATTAATACTATTTTCAAACAGAGCAGTGGTTGTGTTGGAGGATGTGCTAAACCCCCACC
AGCCACCAACATAGAAAGATCTTCAAGAAGGCATCTATCGAGAAGGGGAGTGAGAGGACTGTTTCTGCTGAAAAAGGACTGGTGGTCTTCTAGCCCAGAGGATATGGAGAACAATGAGAGAAATTCCCATTCAATGCGAAGGAATCCATCGGTTGGCAGCACAAGCCAAGCTGAAGAAACACAGTTGGCAAATGACAGTA
CAAACAATACTACTTTCGTAAATCACGCATTGATAATGTGGAATGAAAGAAGAAGGGAGTGGCTAGGAAACCGTTCTCAAAATCGGCCCCAAATGCCAAGAGAACCTATAATAAGTTGGAACACAACATACGATGATTTGCTTGCAACTAACCAGCCTTTTCCACAACCCGTTCCATTACCTGAGATGATTGACTTTCTT
GTGGATGTATGGCATGAAGAAGGGCTTTATGAGTAATAGTGATGCCTTGAAGGGTGATGGAAGAATTGGGAGTTAGCTGTAAAAGGTCCATCTTTTATAATGATTCATGGAAGCAATAAGTTCTTAGCACCCAAGGCAGGCTTTTGCACACACATCCAATTCTGTTCTGAAGCAACGATGTATTTGAGGGATCAAGCTAG
TAACATCTCAGGTCACTACATGAATAATATTTATATGATTAATGGCTGTTATCTTTATAGGGGTC

>TRINITY_DN110770_c0_g1_i3 from sample A2 / Example unspliced isoform:

CGAATCTCAAGCGAAGCCGGCCGGTACTTAATATTTATTGAATAAAGAGGCCCGAGGGTTTCAATCGACGGTCATCTCTGTAAATAAATGATCCTCCGTCGTTACTGTAGCGAAGGCCGTCCTAATGGCAGGGAAGCGAAAGAATGAGACATGGCATTCAACAAGTATCCAATATAGTGAACTTCTTCAAGGACTTCTTT
GACAGGAGTGGTTGTCAAGCTGAACTTCTTAAGGACGTGCTTACATTTGTTATTGTTACAGAACACTGCAAGAAATTAAGATTTCTGTCCTCTGTTTCCTGTGCTTGCATGGGAGCAGTGGTTGTGTTGGAGGATGTGCTAAACCCCCACCAGCCACCAACATAGAAAGATCTTCAAGAAGGCATCTATCGAGAAGGGGA
GTGAGAGGACTGTTTCTGCTGAAAAAGGACTGGTGGTCTTCTAGCCCAGAGGATATGGAGAACAATGAGAGAAATTCCCATTCAATGCGAAGGAATCCATCGGTTGGCAGCACAAGCCAAGCTGAAGAAACACAGTTGGCAAATGACAGTACAAACAATACTACTTTCGTAAATCACGCATTGATAATGTGGAATGAAAG
AAGAAGGGAGTGGCTAGGAAACCGTTCTCAAAATCGGCCCCAAATGCCAAGAGAACCTATAATAAGTTGGAACACAACATACGATGATTTGCTTGCAACTAACCAGCCTTTTCCACAACCCGTTCCATTACCTGAGATGATTGACTTTCTTGTGGATGTATGGCATGAAGAAGGGCTTTATGAGTAATAGTGATGCCTTG
AAGGGTGATGGAAGAATTGGGAGTTAGCTGTAAAAGGTCCATCTTTTATAATGATTCATGGAAGCAATAAGTTCTTAGCACCCAAGGgtacatttctattgtgattcaagagtttaatttgcattgtatgcatgttgagtttcgagcaatcttatcaatagaaactctgttgtattcaaacttacagaggttttacattt
ttttgacattaatgtacgttggaagtaaagactcttgtcagaatcatcttttttgtttcgtttttttcatctcgactgaagcaagatgcatagccaccttagagcatgaatcttctgaaattctctggtgcacttgtgaggaattagccttcaattcccatttcagctatgatgccaggagtgcagggtgtcttttttgt
caaaattacccaggacctgcacctggcctcaactgcaaacccaccattgtgtcaatcatccggctcaatgtccaaagaggtgtagcgatagacaatcctacagattctggggtctgtctatatctaagcaccatctatatgcttttcttaccttgtctttccgcttttttttcctagatgaagaagaaaccttagtaatt
tcttctttgtcattcttgtcgagcaaatctcatgtctattcagattcgtttcaaggggtttcatataaaactaccagttccttcctggctatcgtcacttgccagagttgtcttatgattatttatgtacatggttctttgcagCAGGCTTTTGCACACACATCCAATTCTGTTCTGAAGCAACGATGTATTTGAGGGAT
CAAGCTAGTAACATCTCAGGTCACTACATGAATAATATTTATATGATTAATGGCTGTTATCTTTATAGGGGTC

Intron 2 GTCGGTATTA ... TTTCAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.39	63	30	25
A2	35.06	50	30	24
A3	28.99	60	34	15
A4	45.32	38	40	23
A5	36.54	33	21	17
A6	29.16	158	72	58
A7	20.55	230	57	62
A8	24.43	133	46	40
A9	25.35	187	62	65
A10	20	180	39	51

>TRINITY_DN130956_c4_g2_i3 from sample A1 / Example spliced isoform

CAAGCGAAGCCGGCCGGTACTTAATATTTATTGAATAAAGAGGCCCGAGGGTTTCAATCGACGGTCATCTCTGTAAATAAATGATCCTCCGTCGTTACTGTAGCGAAGGCCGTCCTAATGGCAGGGAAGCGAAAGAATGAGACATGGCATTCAACAAGTATCCAATATAGTGAACTTCTTCAAGGACTTCTTTGACAGGA
GTGGTTGTCAAGCTGAACTTCTTAAGGACGTGCTTACATTTGTTATTGTTACAGAACACTGCAAGAAATTAAGATTTCTGTCCTCTGTTTCCTGTGCTTGCATGGGAGCAGTGGTTGTGTTGGAGGATGTGCTAAACCCCCACCAGCCACCAACATAGAAAGATCTTCAAGAAGGCATCTATCGAGAAGGGGAGTGAGAG
GACTGTTTCTGCTGAAAAAGGACTGGTGGTCTTCTAGCCCAGAGGATATGGAGAACAATGAGAGAAATTCCCATTCAATGCGAAGGAATCCATCGGTTGGCAGCACAAGCCAAGCTGAAGAAACACAGTTGGCAAATGACAGTACAAACAATACTACTTTCGTAAATCACGCATTGATAATGTGGAATGAAAGAAGAAGG
GAGTGGCTAGGAAACCGTTCTCAAAATCGGCCCCAAATGCCAAGAGAACCTATAATAAGTTGGAACACAACATACGATGATTTGCTTGCAACTAACCAGCCTTTTCCACAACCCGTTCCATTACCTGAGATGATTGACTTTCTTGTGGATGTATGGCATGAAGAAGGGCTTTATGAGTAATAGTGATGCCTTGAAGGGTG
ATGGAAGAATTGGGAGTTAGCTGTAAAAGGTCCATCTTTTATAATGATTCATGGAAGCAATAAGTTCTTAGCACCCAAGGGTACATTTCTATTGTGATTCAAGAGTTTAATTTGCATTGTATGCATGTTGAGTTTCGAGCAATCTTATCAATAGAAACTCTGTTGTATTCAAACTTACAGAGGTTTTACATTTTTTTGAC
ATTAATGTACGTTGGAAGTAAAGACTCTTGTCAGAATCATCTTTTTTGTTTCGTTTTTTTCATCTCGACTGAAGCAAGATGCATAGCCACCTTAGAGCATGAATCTTCTGAAATTCTCTGGTGCACTTGTGAGGAATTAGCCTTCAATTCCCATTTCAGCTATGATGCCAGGAGTGCAGGGTGTCTTTTTTGTCAAAATT
ACCCAGGACCTGCACCTGGCCTCAACTGCAAACCCACCATTGTGTCAATCATCCGGCTCAATGTCCAAAGAGGTGTAGCGATAGACAATCCTACAGATTCTGGGGTCTGTCTATATCTAAGCACCATCTATATGCTTTTCTTACCTTGTCTTTCCGCTTTTTTTTCCTAGATGAAGAAGAAACCTTAGTAATTTCTTCTT
TGTCATTCTTGTCGAGCAAATCTCATGTCTATTCAGATTCGTTTCAAGGGGTTTCATATAAAACTACCAGTTCCTTCCTGGCTATCGTCACTTGCCAGAGTTGTCTTATGATTATTTATGTACATGGTTCTTTGCAGCAGGCTTTTGCACACACATCCAATTCTGTTCTGAAGCAACGATGTATTTGAGGGATCAAGCTA
GTAACATCTCAGGCCACTACATGAATAATATTTATATGATTAATGGCTGTTATCTTTATAGGGGTCCTTGTGTACTATAAGATGATATTATGCCGATTATTAAGTTCTGTTATTACGGTGAGTACCCAGCCATTTGGTTAGGACTGGTTATAAATTGATATTCAAATAATGCGAATAAAATCATTATGAGTAGGTCAATA
AATTGTGCTTTTAACCATATCGTTTGTGCTGTTTATATGAATCATGGATGGAGATGTTCTGTAATAACATAGTTCATTGTCAAGCCTGCTTATTCAACAATATTCTAGTCTGCTCTGGAAGCCATTTGTACCTCGCT

>TRINITY_DN130956_c4_g2_i1 from sample A1 / Example unspliced isoform:

CAAGCGAAGCCGGCCGGTACTTAATATTTATTGAATAAAGAGGCCCGAGGGTTTCAATCGACGGTCATCTCTGTAAATAAATGATCCTCCGTCGTTACTGTAGCGAAGGCCGTCCTAATGGCAGGGAAGCGAAAGAATGAGACATGGCATTCAACAAGTATCCAATATAGTGAACTTCTTCAAGGACTTCTTTGACAGGA
GTGGTTGTCAAGCTGAACTTCTTAAGGACGTGCTTACATTTGTTATTGTTACAGAACACTGCAAGAAATTAAGATTTCTGTCCTCTGTTTCCTGTGCTTGCATGGGgtcggtattatacagtgactataagattatgatatcaggatattggatttaatattttttctccactgcaataccatgagataataatggttaa
attttagtttttggtgcacaaattgagtcacttactggcatttcatcatttgttcactatggtctatgtcttataaaattggttatctgagcagtaaatcaccagatattaagctccatttgtttttcattctcattaatactattttcaaacagAGCAGTGGTTGTGTTGGAGGATGTGCTAAACCCCCACCAGCCACC
AACATAGAAAGATCTTCAAGAAGGCATCTATCGAGAAGGGGAGTGAGAGGACTGTTTCTGCTGAAAAAGGACTGGTGGTCTTCTAGCCCAGAGGATATGGAGAACAATGAGAGAAATTCCCATTCAATGCGAAGGAATCCATCGGTTGGCAGCACAAGCCAAGCTGAAGAAACACAGTTGGCAAATGACAGTACAAACAA
TACTACTTTCGTAAATCACGCATTGATAATGTGGAATGAAAGAAGAAGGGAGTGGCTAGGAAACCGTTCTCAAAATCGGCCCCAAATGCCAAGAGAACCTATAATAAGTTGGAACACAACATACGATGATTTGCTTGCAACTAACCAGCCTTTTCCACAACCCGTTCCATTACCTGAGATGATTGACTTTCTTGTGGATG
TATGGCATGAAGAAGGGCTTTATGAGTAATAGTGATGCCTTGAAGGGTGATGGAAGAATTGGGAGTTAGCTGTAAAAGGTCCATCTTTTATAATGATTCATGGAAGCAATAAGTTCTTAGCACCCAAGGGTACATTTCTATTGTGATTCAAGAGTTTAATTTGCATTGTATGCATGTTGAGTTTCGAGCAATCTTATCAA
TAGAAACTCTGTTGTATTCAAACTTACAGAGGTTTTACATTTTTTTGACATTAATGTACGTTGGAAGTAAAGACTCTTGTCAGAATCATCTTTTTTGTTTCGTTTTTTTCATCTCGACTGAAGCAAGATGCATAGCCACCTTAGAGCATGAATCTTCTGAAATTCTCTGGTGCACTTGTGAGGAATTAGCCTTCAATTCC
CATTTCAGCTATGATGCCAGGAGTGCAGGGTGTCTTTTTTGTCAAAATTACCCAGGACCTGCACCTGGCCTCAACTGCAAACCCACCATTGTGTCAATCATCCGGCTCAATGTCCAAAGAGGTGTAGCGATAGACAATCCTACAGATTCTGGGGTCTGTCTATATCTAAGCACCATCTATATGCTTTTCTTACCTTGTCT
TTCCGCTTTTTTTTCCTAGATGAAGAAGAAACCTTAGTAATTTCTTCTTTGTCATTCTTGTCGAGCAAATCTCATGTCTATTCAGATTCGTTTCAAGGGGTTTCATATAAAACTACCAGTTCCTTCCTGGCTATCGTCACTTGCCAGAGTTGTCTTATGATTATTTATGTACATGGTTCTTTGCAGCAGGCTTTTGCACA
CACATCCAATTCTGTTCTGAAGCAACGATGTATTTGAGGGATCAAGCTAGTAACATCTCAGGCCACTACATGAATAATATTTATATGATTAATGGCTGTTATCTTTATAGGGGTCCTTGTGTACTATAAGATGATATTATGCCGATTATTAAGTTCTGTTATTACGGTGAGTACCCAGCCATTTGGTTAGGACTGGTTAT
AAATTGATATTCAAATAATGCGAATAAAATCATTATGAGTAGGTCAATAAATTGTGCTTTTAACCATATCGTTTGTGCTGTTTATATGAATCATGGATGGAGATGTTCTGTAATAACATAGTTCATTGTCAAGCCTGCTTATTCAACAATATTCTAGTCTGCTCTGGAAGCCATTTGTACCTCGCT