NA ----> NA

Intron 1 GTATGTGTCT ... TACTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.69	95	36	23
A2	22	39	17	5
A3	24.87	71	29	18
A4	21.74	54	15	15
A5	33.33	14	6	8
A6	33.33	3	2	1
A7	28.21	14	10	1
A8	14.29	9	0	3
A9	23.81	24	12	3
A10	15.56	19	6	1

>TRINITY_DN98080_c0_g1_i2 from sample A5 / Example spliced isoform

GCCCTATAAGTGGAGGTAAGAGTGAGATTGCAAGGGTTTTAAGGGTTTACACATGCTCATACTTCTTATATTCAAGTCTCCTCCGCCCTCTCGGGTGCCCTAAAAGTGGAAGTAAGCGTAGGACTACAAGGGCTTTCAAGGGTTTATATTTGTATGGATTAGATAATAATAATAAATCTATATCAAGGGATTGTAGCCTC
TCCCACTCCTTCCTCATTTCGATATTTCTCATCCTCCTCGTGGTGGTGACGCGGCTCTGGATCCGTTTTCCTTATCGAATACCTCAGCTGTTTAACCAGGCGTGCTGCTGCGTTATCTTCTTGGATTACATCTATTTGCGGCGGCTTCTTCAAAGGCACGAAATTAAATTGAAGGTTTCCTGAAACTTGATATGGCTCAT
TTTCCCACGGTGTTTCCATTTGACAGAAAAAGCAAATGAACTATCGTAGGGATCACATATTAGATTTGGAGCTTTCCAGCTGCGTGTTAGTGTGCTTTACATTGAACTGGTTATTTGTGGAATCGATTTTTTAATTCAGAAATGGGTTTTGACAATGAGAGTATCTTGAATATACAATCACTTCCAGGAGAATATTTCTG
TCCTGTTTGTCGCCAACTTGTTTATCCTAATGAGGCTTTGCAAACACAATGTACACATCTGTATTGCAAGCCTTGCTTAACATATTGTGTGAGTACTACACGGGCCTGTGCTTATGATGGCTATCTGGTTACTGATACAGATTCAAAGCCGTTACAAGAGTCAAATCCGTCACTGGCTGAAACAATAGGCCGTGTAGCTG
TTCATTGTCTATATCATAAGAGTGGCTGCACTTGGCAGGGACCTCTCTCAGAGAGTATTCTTCATGGAAATAGTTGTCCATATGGCTCCTCACCTGTTGTCTGCAACAGATGTGGGACCCAAATTATTCACAGACAAGTGCAGGAGCATGCTCAGAATTGCCCTGGGACCCAAGGTCAGGGCCAGCAACCAGAAAATGCT
CCTCCAACCCAAAGTGCTGTAGTTGCAGCAGCTTATGAGCAGAATCCAGTGGCACAAGCTGCTGGTCAACAATATCCAGCCCAAACACCAACGCAAGTGGCTCAAGCTCCAGTTTCTCAAGCTACAAATTATGGAATGGGGCAAGATCAGCATCAGGGG

>TRINITY_DN98080_c0_g1_i1 from sample A5 / Example unspliced isoform:

GCCCTATAAGTGGAGGTAAGAGTGAGATTGCAAGGGTTTTAAGGGTTTACACATGCTCATACTTCTTATATTCAAGTCTCCTCCGCCCTCTCGGGTGCCCTAAAAGTGGAAGTAAGCGTAGGACTACAAGGGCTTTCAAGGGTTTATATTTGTATGGATTAGATAATAATAATAAATCTATATCAAGGGATTGTAGCCTC
TCCCACTCCTTCCTCATTTCGATATTTCTCATCCTCCTCGTGGTGGTGACGCGGCTCTGGATCCGTTTTCCTTATCGAATACCTCAGCTGTTTAACCAGGCGTGCTGCTGCGTTATCTTCTTGGATTACATCTATTTGCGGCGGCTTCTTCAAAGGCACGAAATTAAATTGAAGGTTTCCTGAAACTTGATATGGCTCAT
TTTCCCACGGTGTTTCCATTTGACAGAAAAAGCAAATGAACTATCGTAGGGATCACATATTAGATTTGGAGCTTTCCAGCTGCGTGTTAGTGTGCTTTACATTGAACTGGTTATTTGTGGAATCGATTTTTTAATTCAGAAATGGGTTTTGACAATGAGAGTATCTTGAATATACAATCACTTCCAGGAGAATATTTCTG
TCCTGTTTGTCGCCAACTTGTTTATCCTAATGAGGCTTTGCAAACACAATGTACACATCTGTATTGCAAGCCTTGCTTAACATATTGTGTGAGTACTACACGGGCCTGTGCTTATGATGGCTATCTGGTTACTGATACAGATTCAAAGgtatgtgtctgccctctattacaatggtcacaaataattaagtaattatgaa
attactttataccttttctgatgattgctgctttactctgcagCCGTTACAAGAGTCAAATCCGTCACTGGCTGAAACAATAGGCCGTGTAGCTGTTCATTGTCTATATCATAAGAGTGGCTGCACTTGGCAGGGACCTCTCTCAGAGAGTATTCTTCATGGAAATAGTTGTCCATATGGCTCCTCACCTGTTGTCTGCA
ACAGATGTGGGACCCAAATTATTCACAGACAAGTGCAGGAGCATGCTCAGAATTGCCCTGGGACCCAAGGTCAGGGCCAGCAACCAGAAAATGCTCCTCCAACCCAAAGTGCTGTAGTTGCAGCAGCTTATGAGCAGAATCCAGTGGCACAAGCTGCTGGTCAACAATATCCAGCCCAAACACCAACGCAAGTGGCTCAA
GCTCCAGTTTCTCAAGCTACAAATTATGGAATGGGGCAAGATCAGCATCAGGGG

Intron 2 GTTAGTATAA ... TTTACCGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55	9	11	4
A2	66.67	4	8	2
A3	66.67	5	10	2
A4	72.73	3	8	0
A5	50	4	4	0
A6	-1	0	0	0
A7	66.67	3	6	0
A8	-1	0	0	0
A9	100	0	1	0
A10	100	0	1	0

>TRINITY_DN127833_c1_g2_i2 from sample A1 / Example spliced isoform

AAAACTTTTCACTCTGTTTCTTTATATTTTTGAATGATAGGGCGGATAAGTTCACTGGACTCCAATACTAGGGTTTATCAACTCTAGAAGAATGGCTAAGGGCTTTGAGGTTTGGGCGTTTGCTGCTGGGGTTTTGGCATTTCCAGGGTTTTTCTAACATATAACTTGAGATTTCATGGTTATTTGCGTGGCCCGCCCAT
TGCTGTTGCGAGGCAAACGTTTTACTATTTTTCCTTTTCACGATCTTTTTCTTCCTTTCGAAATATTTGGGGGACTGATATCTTTTTATCTAAATTTAGGTATTTGGGTTGGGGGGAGCTGCTTTGAGAAATAAGTACCTTAGGTTGGGGCTGCCCTGTACAACACTGCGTTTTTTAGACATGCCATTCGGGTGGGTCAG
GACACGGCAATAAATGAATGCTACTAGATGGCTTAGTACTTGGTTAATCGCACCAGCGAATCGAAGCTCATTTAGTAAAAGGCTTCGTCCTGAACTCTGTCGCAGTCTTGGCATTCATGCAGCCCCTGCATGGTTCGGAGGGGCTTCCTATAAAACCGGATTTGAAAAAATCATATTATGCATTGCATGCCAGCCACATG
GC

>TRINITY_DN127833_c1_g2_i6 from sample A1 / Example unspliced isoform:

CGGGCGCCCTATAAGTGGAGGTAAGAGTGAGATTGCAAGGGTTTTAAGGGTTTACACATGCTCATACTTCTTATATTCAAGTCTCCTCCGCCCTCTCGGGTGCCCTAAAAGTGGAAGTAAGCGTAGGACTACAAGGGCTTTCAAGGGTTTATATTTGTATGGATTAGATAATAATAATAAATCTATATCAAGGGATTGTA
GCCTCTCCCACTCCTTCCTCATTTCGATATTTCTCATCCTCCTCGTGGTGGTGACGCGGCTCTGGATCCGTTTTCCTTATCGAATACCTCAGCTGTTTAACCAGGCGTGCTGCTGCGTTATCTTCTTGGATTACATCTATTTGCGGCGGCTTCTTCAAAGGTATTTGGGTTGGGGGGAGCTGCTTTGAGAAATAAGTACC
TTAGGTTGGGGCTGCCCTGTACAACACTGCGTTTTTTAGACATGCCATTCGGGTGGGTCAGGACACGGCAATAAATGAATGCTACTAGATGGCTTAGTACTTGGTTAATCGCACCAGCGAATCGAAGCTCATTTAGTAAAAGGCTTCGTCCTGAACTCTGgttagtataattcttttggcgccgtagccatggagctgga
ttgatcttattgttgatgggagatgcttacacatttgtgttcaatccacccattatgatttcttgttaacccataagttctaatttatcagcggcatatttaaaggagagtcagtgggactgaaaattacttcctttagacagtcattactggattatacctttgtccagttgccccatctattgtcatttgtggacatt
aagaacatgttcttatctgcatactaatgtatgtctaaccgttataatttttaccgtagTCGCAGTCTTGGC

Intron 3 GTGTGTAGGC ... CTAAATTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.38	42	23	9
A2	37.5	30	18	4
A3	44.83	32	26	10
A4	54.55	20	24	1
A5	42.86	16	12	5
A6	0	6	0	6
A7	37.5	15	9	4
A8	50	2	2	0
A9	50	10	10	1
A10	46.43	15	13	1

>TRINITY_DN113249_c0_g2_i5 from sample A3 / Example spliced isoform

GCGCCCTATAAGTGGAGGTAAGAGTGAGATTGCAAGGGTTTTAAGGGTTTACACATGCTCATACTTCTTATATTCAAGTCTCCTCCGCCCTCTCGGGTGCCCTAAAAGTGGAAGTAAGCGTAGGACTACAAGGGCTTTCAAGGGTTTATATTTGTATGGATTAGATAATAATAATAAATCTATATCAAGGGATTGTAGCC
TCTCCCACTCCTTCCTCATTTCGATATTTCTCATCCTCCTCGTGGTGGTGACGCGGCTCTGGATCCGTTTTCCTTATCGAATACCTCAGCTGTTTAACCAGGCGTGCTGCTGCGTTATCTTCTTGGATTACATCTATTTGCGGCGGCTTCTTCAAAGGTATTTGGGTTGGGGGGAGCTGCTTTGAGAAATAAGTACCTTA
GGTTGGGGCTGCCCTGTACAACACTGCGTTTTTTAGACATGCCATTCGGGTGGGTCAGGACACGGCAATAAATGAATGCTACTAGATGGCTTAGTACTTGGTTAATCGCACCAGCGAATCGAAGCTCATTTAGTAAAAGGCTTCGTCCTGAACTCTGGCACGAAATTAAATTGAAGGTTTCCTGAAACTTGATATGGCTC
ATTTTCCCACGGTGTTTCCATTTGACAGAAAAAGCAAATGAACTATCGTAGGGATCACATATTAGATTTGGAGCTTTCCAGCTGCGTGTTAGTGTGCTTTACATTGAACTGGTTATTTGTGGAATCGATTTTTTAATTCAGAAATGGGTTTTGACAATGAGAGTATCTTGAATATACAATCACTTCCAGGAGAATATTTC
TGTCCTGTTTGTCGCCAACTTGTTTATCCTAATGAGGCTTTGCAAACACAATGTACACATCTGTATTGCAAGCCTTGCTTAACATATTGTGTGAGCACTACACGGGCCTGTGCTTATGATGGCTATCTGGTTACTGATACAGATTCAAAGCCGTTACAAGAGTCAAATCCGTCACTGGCTGAAACAATAGGCCGTGTAGC
TGTTCATTGTCTATATCATAAGAGTGGCTGCACTTGGCAGGGACCTCTCTCAGAGAGTATTCTTCATGGAAATAGTTGTCCATATGGCTCCTCACCTGTTGTCTGCAACAGATGTGGGACCCAAATTATTCACAGACAAGTGCAGGAGCATGCTCAGAATTGCCCTGGGACCCAAGGTCAGGGCCAGCAACCAGAAAATG
CTCCTCCAACCCAAAGTGCTGTAGTTGCAGCAGCTTATGAGCAGAATCCAGTGGCACAAGCTGCTGGTCAACAATATCCAGCCCAAACACCAACGCAAGTGGCTCAAGCTCCAGTTTCTCAAGCTACAAATTATGGAATGGGGCAAGATCAGCATCAGGGAAATCTAACTATGCCACAGGCTGCAGCCCAAACATCTGCC
ACTGATCAGTGGTATCAACAACAATACCAACAGTACTACCAACAATACCCACAGTATGATCAGTATCAGCAGTACCAGCAATATGACCAGTATCAGCAGCAAGCCCAAGCCCAGGCCCAGGCCCAGGCCCAGGCACAGGCACAACCCCCACCCCCCCCCCCACCCCCCACCCCC

>TRINITY_DN113249_c0_g2_i4 from sample A3 / Example unspliced isoform:

GCGCCCTATAAGTGGAGGTAAGAGTGAGATTGCAAGGGTTTTAAGGGTTTACACATGCTCATACTTCTTATATTCAAGTCTCCTCCGCCCTCTCGGGTGCCCTAAAAGTGGAAGTAAGCGTAGGACTACAAGGGCTTTCAAGGGTTTATATTTGTATGGATTAGATAATAATAATAAATCTATATCAAGGGATTGTAGCC
TCTCCCACTCCTTCCTCATTTCGATATTTCTCATCCTCCTCGTGGTGGTGACGCGGCTCTGGATCCGTTTTCCTTATCGAATACCTCAGCTGTTTAACCAGGCGTGCTGCTGCGTTATCTTCTTGGATTACATCTATTTGCGGCGGCTTCTTCAAAGgtgtgtaggctcagctagggtttatgaaaacttttcactctgt
ttctttatatttttgaatgatagggcggataagttcactggactccaatactagggtttatcaactctagaagaatggctaagggctttgaggtttgggcgtttgctgctggggttttggcatttccagggtttttctaacatataacttgagatttcatggttatttgcgtggcccgcccattgctgttgcgaggcaaa
cgttttactatttttccttttcacgatctttttcttcctttcgaaatatttgggggactgatatctttttatctaaatttagGTATTTGGGTTGGGGGGAGCTGCTTTGAGAAATAAGTACCTTAGGTTGGGGCTGCCCTGTACAACACTGCGTTTTTTAGACATGCCATTCGGGTGGGTCAGGACACGGCAATAAATGA
ATGCTACTAGATGGCTTAGTACTTGGTTAATCGCACCAGCGAATCGAAGCTCATTTAGTAAAAGGCTTCGTCCTGAACTCTGGCACGAAATTAAATTGAAGGTTTCCTGAAACTTGATATGGCTCATTTTCCCACGGTGTTTCCATTTGACAGAAAAAGCAAATGAACTATCGTAGGGATCACATATTAGATTTGGAGCT
TTCCAGCTGCGTGTTAGTGTGCTTTACATTGAACTGGTTATTTGTGGAATCGATTTTTTAATTCAGAAATGGGTTTTGACAATGAGAGTATCTTGAATATACAATCACTTCCAGGAGAATATTTCTGTCCTGTTTGTCGCCAACTTGTTTATCCTAATGAGGCTTTGCAAACACAATGTACACATCTGTATTGCAAGCCT
TGCTTAACATATTGTGTGAGCACTACACGGGCCTGTGCTTATGATGGCTATCTGGTTACTGATACAGATTCAAAGCCGTTACAAGAGTCAAATCCGTCACTGGCTGAAACAATAGGCCGTGTAGCTGTTCATTGTCTATATCATAAGAGTGGCTGCACTTGGCAGGGACCTCTCTCAGAGAGTATTCTTCATGGAAATAG
TTGTCCATATGGCTCCTCACCTGTTGTCTGCAACAGATGTGGGACCCAAATTATTCACAGACAAGTGCAGGAGCATGCTCAGAATTGCCCTGGGACCCAAGGTCAGGGCCAGCAACCAGAAAATGCTCCTCCAACCCAAAGTGCTGTAGTTGCAGCAGCTTATGAGCAGAATCCAGTGGCACAAGCTGCTGGTCAACAAT
ATCCAGCCCAAACACCAACGCAAGTGGCTCAAGCTCCAGTTTCTCAAGCTACAAATTATGGAATGGGGCAAGATCAGCATCAGGGAAATCTAACTATGCCACAGGCTGCAGCCCAAACATCTGCCACTGATCAGTGGTATCAACAACAATACCAACAGTACTACCAACAATACCCACAGTATGATCAGTATCAGCAGTAC
CAGCAATATGACCAGTATCAGCAGCAAGCCCAAGCCCAGGCCCAGGCCCAGGCCCAGGCACAGGCACAACCCCCACCCCCCCCCCCACCCCCCACCCCC