sp|Q9XFX4|CARDB_CYNCA ----> Procardosin-B

Intron 1 GTTCTCTCTC ... GCATTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.73	3	3	8
A2	62.5	6	1	10
A3	85.71	2	5	12
A4	83.33	1	0	5
A5	70.59	5	4	12
A6	73.33	4	7	11
A7	70.27	11	9	26
A8	61.67	23	9	37
A9	72	7	5	18
A10	56	22	1	28

>TRINITY_DN44240_c1_g2_i9 from sample A6 / Example spliced isoform

GCTATTATGAATAGGGTTAGGGTCTGAGACTCTGAATCTCTTCATTTCTTTACTCTCTGCCCTGCGCGCGCTGACATGTTTCTGGCGTTTTGTAGTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACTTCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATT
AGGACTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAGCGACCTAATGCGTTGCCGATTCTCTGAAATGTATCATAAGGTCAAATGTGTATCCCTCGCAATTCGAATGGAAATGTATATCAGGCTGAATTCGAATCATTGCTCTGCGTTGACAGTGGGTACTATTATGCGTCGTAAGAAGAAATTTGGCCG
AAAAGAAGAAGAGGCTAAAGTCACCGGCCTTACAAGAAGCATAAGAGAGCAACACTTGATTTATAGTGTGACTGGAGATCAAGGGTTACAGAAGTAACGACCAGACCTGGAAAATGCATGCTAATGGGTGATGCCAACCTTCTGAAGCA

>TRINITY_DN44240_c1_g2_i7 from sample A6 / Example unspliced isoform:

GCTATTATGAATAGGGTTAGGGTCTGAGACTCTGAATCTCTTCATTTCTTTACTCTCTGCCCTGCGCGCGCTGgttctctctctcacgtactgtaatttagagttgtgcattgaagagtagtactctgtatattctgtgttttaaattttaatgaccgtgtgcactgtgtttgcatttgtagACATGTTTCTGGCGTTTT
GTAGTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACTTCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGACTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAGCGACCTAATGCGTTGCCGATTCTCTGAAATGTATCATAAGGTCA
AATGTGTATCCCTCGCAATTCGAATGGAAATGTATATCAGGCTGAATTCGAATCATTGCTCTGCGTTGACAGTGGGTACTATTATGCGTCGTAAGAAGAAATTTGGCCGAAAAGAAGCAGAGGCTAAGTACAAAAATGGTCTATCGAAGTTATTATTTTATTTTTTATTTTTATTTTTTTTTTATTATAAATTGACTTTT
TCCAGCGAA

Intron 2 GTTAGGGTCT ... GCATTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.2	2.4	0	8
A2	71.43	4	2	10
A3	85.71	2	0	12
A4	83.33	1	0	5
A5	70.59	5	0	12
A6	73.33	4	0	11
A7	72.22	10	4	26
A8	63.79	21	1	37
A9	78.26	5	2.8	18
A10	64	15.8	0	28

>TRINITY_DN44685_c3_g1_i5 from sample A7 / Example spliced isoform

AGGGGAGAATCGCTATTATGAATAGGACATGTTTCTGGCGTTTTGTAGTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACTTCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGACTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAG
CGACCTAATGCGTTGCCGATTCTCTGAAATGTATCATAAGGTCAAATGTGTATCCCTCGCAATTCGAATGGAAATGTATATCAGGCTGAATTCGAATCATTGCTCTGCGTTGACAGTGGGTACTATTATGCGTCGCAAGAAGAAATTTGGCCGAAAAGAAGCAGAGGCTAAGTACAAAAATGGTCTATCGAAGTTATTAT
TTTTATTTTTTTTATTTTTTTTTATTTTTATTATAAATTGACTTTATTTTCATTTTTTATCTAAATGTTCTCAGTCTAACCATCAATGCTGACTTTGAGACGTCAGCATTTAAATCTAACCGTCAGGATTTTGTTTCAAAGAAATAGGTTCAATATTGACGTTTATTTCTGATTTATGAGTAAATTAAATAAATCTTACC
ACTCTATTTTCCGTTCCAGTAAATTAAATAATTATATGGTTATTGAAATCATTCTTCTCTTGATTTACTGCGTTTTGTATTTTAAC

>TRINITY_DN44685_c3_g1_i3 from sample A7 / Example unspliced isoform:

AATCGCTATTATGAATAGGgttagggtctgagactctgaatctcttcatttctttactctctgccctgcgcgcgctggttctctctctcacgtactgtaatttagagttgtgcattgaagagtagtactctgtatattctgtgttttaaattttaatgaccgtgtgcactgtgtttgcatttgtagACATGTTTCTGGCG
TTTTGTAGTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACTTCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGACTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAGCGACCTAATGCGTTGCCGATTCTCTGAAATGTATCATAAG
AGTCACCGGCCTTACAAGAAGCATAAGAGAGCAACACTTGATTTATAGTGTGACTGGAGATCAAGGGTTACAGAAGTAACGACCAGACCTGGAAAATGCATGCTAATGGGTGATGCCAACCTTCTGAAGCTTTCATTATCGTCTTCCTCGTTCTTTTATAATCCGGATAGAGAGGCTTACTACCAGGTGATACCGAGTTG
GGTAATTCACTAGTGGATGTCTTATACTCTTTCCAATCAACCTTTCTACATTACTTGGTGGCGTGTTCATATTTCAGTGGTGTAATTCAGTATTTCAGTGGTGCAGTTTCTAGATTTTTGTCTCCAGATTATGTCACAGTTGTGGACTTCGTTGTGAAATTCCAGATTTTTACCATTCTTGTTGTCAAAGTTTGACGGTC
TTCTTGGACTTGGATTTTCGAGACAAGGTTGCTCCAGTAGTGGGTTATAACATGGTCAACCAAGGTAGGGTCAAAAAGCCAGTCTTTTCTTTTGGATGAACGGGACCTGGGACGAGGAAGATGGATGAGAGATTGTATTTGGTGGTGTTGCTCCTTCAACACTTCTAAGGGTGAACATAAATATGTGCTTATTACTCGAA
AGGGTTAGTGGCAGTTTGCTATGCGAGACTTTCTCGTTGATAATCAAGCAACAGTTATCTGTGCTGGAGGTTGGAGATTCTGGAATTTCTTCGATGGTCGATCCTTTAGGCATTATAACACACATCAACCAGGCATTAGGTGTTACTAACTAGGTTGGCTTGTTATTTATATATTTGTGCAAATGTATGCATTGATAAAT
GTCAGTTCCTTGTTATGTTATGTTGACTATAAATTGTGCCTCTTATATGTACACTAGACTCTCTCTCTCATAAGGATTGCGACTACCTTGTCTTATTCATATGTATGAATAAGAGTATCTCTTATGTGCTCATTTCATCGATTTTGAACCAACTTTTTGAATTATATTCAAAGCTTAAAAAGTGGAATGAGGAAGAACTC
ATGGGCTTTAAATCTACAGAAGCAAAGCAGGAAGTTTTTGAATGGGAAAGAAGAAGAGAGCTTCTTCTATTATAGGAGGCAGAATGGAGATTAAAAAGCAGGGCTTTGTGGATGGCCAAAGGAGC

Intron 3 GTAATGGCGT ... GGTTTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.5	9	15	2
A2	76.19	5	16	1
A3	60	4	6	7
A4	88.89	1	8	1
A5	57.14	3	4	0
A6	40	12	8	1
A7	25.71	26	9	3
A8	30	21	9	0
A9	36	16	9	8
A10	28.21	28	11	0

>TRINITY_DN92753_c0_g1_i1 from sample A3 / Example spliced isoform

GCCCTGCGCGCGCTGGTTCTCTCTCTCACGTACTGTAATTTAGAGTTGTGCATTGAAGAGTAGTACTCTGTATATTCTGTGTTTTAAATTTTAATGACCGTGTGCACTGTGTTTGCATTTGTAGACATGTTTCTGGCGTTTTGTAGTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACT
TCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGACTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAGCGACCTAATGCGTTG

>TRINITY_DN92753_c0_g1_i2 from sample A3 / Example unspliced isoform:

GCCCTGCGCGCGCTGGTTCTCTCTCTCACGTACTGTAATTTAGAGTTGTGCATTGAAGAGTAGTACTCTGTATATTCTGTGTTTTAAATTTTAATGACCGTGTGCACTGTGTTTGCATTTGTAGACATGTTTCTGGCGTTTTGTAGTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACT
TCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGACTCAAACTTACTAGgtaatggcgttgccatagaatcttttgaatttttagataacatatgatcaatgaagttttgataaatctttgcactggataggtttgaaaactatttcttagattccaaacttttgtcgcgatttatataa
tctaaagacttgagaaatctttatcagaaaaagttgcttgaatcttaagaaaaagagttttttttgtgatttgtcttaatcagaaaaggttgcttgaatcgtacgaaaaagagtttcctgaatcgcacacattccagccactatgagattaacttgtttactagaattagaatggcaattttctaggtctggaataagta
gcttttattatttttgtctttactgcaaactattgacgcttcttcttatttattcgcaaatcaaataaatcttaccactccagtttgctttctgatctggtttaacagGAAATTAAATAATCATATGCGATCATTCTTCTCTTGTTTAGTAGAATGATAATTTTCTAGTTAAGGAGCTTTGATTTTTTGTTTTTCCTGCA
AATTATTGACGCTTTTTCTGATTTATTGGCAAATCAAATAAATCTTACCAC

Intron 4 GTACTGTAAT ... CGTTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85	1.5	3	8
A2	84.62	2	5	11
A3	75	5	5	15
A4	83.33	1	0	5
A5	86.67	2	4	13
A6	81.25	3	9	13
A7	64.89	19.5	13	36
A8	65.7	23.5	10	45
A9	62.67	15.5	8	26
A10	62.93	22.5	3	38

>TRINITY_DN44685_c3_g1_i10 from sample A7 / Example spliced isoform

AATCGCTATTATGAATAGGGTTAGGGTCTGAGACTCTGAATCTCTTCATTTCTTTACTCTCTGCCCTGCGCGCGCTGGTTCTCTCTCTCACTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACTTCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGA
CTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAGCGACCTAATGCGTTGCCGATTCTCTGAAATGTATCATAAGGTCAAATGTGTATCCCTCGCAATTCGAATGGAAATGTATATCAGGCTGAATTCGAATCATTGCTCTGCGTTGACAGTGGGTACTATTATGCGTCGCAAGAAGAAATTTGGCCGAAAA
GAAGCAGAGGCTAAGTACAAAAATGGTCTATCGAAGTTATTATTTTATTTTTTATTTTTATTTTTTTTTTATTATAAATTGACTTTTTCCAGCGAAAAAAACTACTATTGATATTCATATTGAATCAAATAGTACAATGATTGTCTTGATTCTGCTATGGAATCATATACTTCTGAAATATAACAGACATGGAAGCAAAG
AAAGCTTCAACATAGATCAGCCACCCAATAATATGACAGTTTTGTTTACAACACAATCCAGATCAACATATCACAAGAAGACAAAAATTGTC

>TRINITY_DN44685_c3_g1_i3 from sample A7 / Example unspliced isoform:

AATCGCTATTATGAATAGGGTTAGGGTCTGAGACTCTGAATCTCTTCATTTCTTTACTCTCTGCCCTGCGCGCGCTGGTTCTCTCTCTCACgtactgtaatttagagttgtgcattgaagagtagtactctgtatattctgtgttttaaattttaatgaccgtgtgcactgtgtttgcatttgtagacatgtttctggcg
ttttgtagTTGGATTTGCGATAATTGCGAAAGGAGATGAGAAGATGACGTCCCCATCGTACTTCTCCAACATTCTTTAGATCGTGTACGGTTTCGTCTGCGATCGTCTCGATTAGGACTCAAACTTACTAGGAAATTAAATAATCATATGCTTAATGAAGCGACCTAATGCGTTGCCGATTCTCTGAAATGTATCATAAG
AGTCACCGGCCTTACAAGAAGCATAAGAGAGCAACACTTGATTTATAGTGTGACTGGAGATCAAGGGTTACAGAAGTAACGACCAGACCTGGAAAATGCATGCTAATGGGTGATGCCAACCTTCTGAAGCTTTCATTATCGTCTTCCTCGTTCTTTTATAATCCGGATAGAGAGGCTTACTACCAGGTGATACCGAGTTG
GGTAATTCACTAGTGGATGTCTTATACTCTTTCCAATCAACCTTTCTACATTACTTGGTGGCGTGTTCATATTTCAGTGGTGTAATTCAGTATTTCAGTGGTGCAGTTTCTAGATTTTTGTCTCCAGATTATGTCACAGTTGTGGACTTCGTTGTGAAATTCCAGATTTTTACCATTCTTGTTGTCAAAGTTTGACGGTC
TTCTTGGACTTGGATTTTCGAGACAAGGTTGCTCCAGTAGTGGGTTATAACATGGTCAACCAAGGTAGGGTCAAAAAGCCAGTCTTTTCTTTTGGATGAACGGGACCTGGGACGAGGAAGATGGATGAGAGATTGTATTTGGTGGTGTTGCTCCTTCAACACTTCTAAGGGTGAACATAAATATGTGCTTATTACTCGAA
AGGGTTAGTGGCAGTTTGCTATGCGAGACTTTCTCGTTGATAATCAAGCAACAGTTATCTGTGCTGGAGGTTGGAGATTCTGGAATTTCTTCGATGGTCGATCCTTTAGGCATTATAACACACATCAACCAGGCATTAGGTGTTACTAACTAGGTTGGCTTGTTATTTATATATTTGTGCAAATGTATGCATTGATAAAT
GTCAGTTCCTTGTTATGTTATGTTGACTATAAATTGTGCCTCTTATATGTACACTAGACTCTCTCTCTCATAAGGATTGCGACTACCTTGTCTTATTCATATGTATGAATAAGAGTATCTCTTATGTGCTCATTTCATCGATTTTGAACCAACTTTTTGAATTATATTCAAAGCTTAAAAAGTGGAATGAGGAAGAACTC
ATGGGCTTTAAATCTACAGAAGCAAAGCAGGAAGTTTTTGAATGGGAAAGAAGAAGAGAGCTTCTTCTATTATAGGAGGCAGAATGGAGATTAAAAAGCAGGGCTTTGTGGATGGCCAAAGGAGC