NA ----> NA

Intron 1 GTAAGAAGCT ... TGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	7	12	2
A2	57.58	7	15	4
A3	66.67	5	16	4
A4	73.33	2	9	2
A5	60	5	10	5
A6	42.08	53	44	33
A7	53.64	35	41	40
A8	39.53	52	12	56
A9	43.5	63	52	45
A10	51.45	42	50	39

>TRINITY_DN43056_c0_g4_i4 from sample A9 / Example spliced isoform

GAGAGATATCTTTAGTTTATGACCCTCTTATGGCCCAATCAATCAGGAAATATTGGTCAAACAAGAGAGTCAGCGTTTCGAATTGCCCTTCAGCGCTATAAAACTATCGATCAGGAAATATTCGACACCAAACATGAAGCTGGTCTACCTGCATTCCTCCCAACAGGCTTAACCGTCCAGTTAAATAACTTTGATTCTTG
CAGGTGAATACTTTGATTGTCGCAAGAAGCTGGCCTACATTCATTCATTGCAACAGGCTTAACCTTCCCGTTAAATAACTTTGATTCTTGCAGATGAATACTTAGATTTTGGACTTGAACTTCTTCGTAGTTATTTGAAGCTTGGGCCTGTTGCATTTGATTGGAAGAAGCTGTATCAGGAAAATGGTAAACGCGGCCCT
AGGCTGTGCTATTCCATTGGACGACTGTGTATCAGCGTAGGCACGGCGGCTTGGATTTGGCTTGATTGATCATTCTGGGAACTTTGAAATTGATATTGTGATCTTGATCTCTTTGTCT

>TRINITY_DN43056_c0_g4_i5 from sample A9 / Example unspliced isoform:

GAGAGATATCTTTAGTTTATGACCCTCTTATGGCCCAATCAATCAGGAAATATTGGTCAAACAAGAGAGTCAGCGTTTCGAATTGCCCTTCAGCGCTATAAAACTATCGATCAGGAAATATTCGACACCAAACATGAAGCTGGTCTACCTGCATTCCTCCCAACAGGCTTAACCGTCCAGTTAAATAACTTTGATTCTTG
CAGGTGAATACTTTGATTGTCGCAAGAAGCTGGCCTACATTCATTCATTGCAACAGGCTTAACCTTCCCGTTAAATAACTTTGATTCTTGCAGATGAATACTTAGATTTTGGACTTGgtaagaagctggcttacattcattccttgcgagtggcttaaccttccagtaaagaagtttgattcttgcatgtgaatactttg
attctcgcaaggtgaatatggcaatgagattggcaaaggacaggcgtggatacttttggagaacagcagagggttctccttcccagataaataagcaaatttgcgaggagcctgtttcaccatggacgcgagacattcttacgaatctggatgattatgagctagctcgtgatcgcggtgaattatataacaattttcag
ggctgcggaagctggacaaagtcgattaaatccccaaacgttgtgggatttagtcctgggagtgggcaatttgtaggacatcagcagcacagggaagacaatcccattattttctcatcgcaacatactggtctttccttctctggctctaatcgcttaaaccctaattattccaagaatcctggacatgggattcccag
cagcagttttgttcttctctcctctagctctaatcccttaaaccctaactatcccatttatggacatgagattcccggcagtagttttgttcaatctcttcgtaatgtcccaacttcaatgttcatgccttcctcatcgttacaaactagtcttccctcctctacgtataatcccttaaaccctaattatccccagaatc
ccatttatggacctgagattcccagcagcaattttgttcaatctcttcccaatgtcccaacttcaatgttcatgccttcctctttgggtgcacatcccggcccacaatacttaagttcatcaaacactctgcctccttcttcgtcatcacatgaaatagaaactctgatgggtggagcacagatattcgccaatatccac
aaccctaatcaaaaacatttaacttcgtttgttagcattatgaactctggtacggaaattttaaacaaactgaaagccgatgctgcagcgaacatcccatatcggccatatcagttcgatgtgggcaatgttgtagaacaaaatacggagctcctgcgcttcttgaaagaaacaatagaaccaactcgctcttcaaaaaa
cgtattgcctgaaacttcacagcaacaacagcacagtcaagaaccatgtggagatggggagaaagaacagattgagcaacagtagtaggttattgaagagggattgaaaatcgtttatttagtttgcagaatcgttaaattgaggattatagtctttctttattataatttcacaaataacttgttcagatatattattt
atgtgcaaaccctaattaatgttatgtttttccgttcgctagattttatttcaatttgttgaaaccctaattactgaacttgttgtgcagAACTTCTTCGTAGTTATTTGAAGCTTGGGCCTGTTGCATTTGATTGGAAGAAGCTGTATCAGGAAAATGGTAAACGCGGCCCTAGGCTGTGCTATTCCATTGGACGACTG
TGTATCAGCGTAGGCACGGCGGCTTGGATTTGGCTTGATTGATCATTCTGGGAACTTTGAAATTGATATTGTGATCTTGATCTCTTTGTCT

Intron 2 GTAGGTTATT ... TGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.86	7.1	2	2
A2	14.95	13.5	3.1	2.2
A3	46.06	6.1	0	4
A4	47.27	2.5	2	2
A5	51.51	7.3	0	5
A6	51.59	37.9	6.5	33
A7	47.78	50.8	20.6	39.1
A8	50.23	64.3	19.5	55.5
A9	46.19	68	14.7	45.5
A10	50.67	41.4	13.8	37.5

>TRINITY_DN42837_c0_g1_i10 from sample A8 / Example spliced isoform

GGTGAATACTTTGATTGTCGCTAGAAGCTGGCCTACATTCATTCATTGCAACAGGCTTAACCTTCCCGTTAAATAACTTTGATTCTTGCAGATGAATACTTAGATTTTGGTAAGAAGCTGGCTTACATTCATTCCTTGCGAGTGCCTTAACCTTCCAGTAAATAAGTTTGATTCTTGCATGTGAATACTTTGATTCTCGC
AAGGTGAATATGGCAATGAGATTGGCAAAGGACAGGCGTGGATACTTTTGGAGAACAGCAGAGGGTTCTCCTTCCCAGATAAATAAGCAAATTTGCGAGGAGCCTGTTTCACCATGGACGCGAGACATTCTTACGAATCTGGATGATTATGAGCTAGCTCGTGATCGCGGTGAATTATATAACAATTTTCAGGGCTGCGG
AAGCTGGACAAAGTCGATTAAATCCCCAAACGTTGTGGGATTTAGTCCTGGGAGTGGGCAATTTGTAGGACATCAGCAGCACAGGGAAGACAATCCCATTATTTTCTCATCGCAACATACTGGTCTTTCCTTCTCTGGCTCTAATCGCTTAAACCCTAATTATTCCAAGAATCCTGGACATGGGATTCCCAGCAGCAGTT
TTGTTCTTCTCTCCTCTAGCTCTAATCCCTTAAACCCTAACTATCCCATTTATGGACATGAGATTCCCGGCAGTAGTTTTGTTCAATCTCTTCGTAATGTCCCAACTTCAATGTTCATGCCTTCCTCATCGTTACAAACTAGTCTTCCCTCCTCTACGTATAATCCCTTAAACCCTAATTATCCCCAGAATCCCATTTAT
GGACCTGAGATTCCCAGCAGCAATTTTGTTCAATCTCTTCCCAATGTCCCAACTTCAATGTTCATGCCTTCCTCTTTGGGTGCACATCCCGGCCCACAATACTTAAGTTCATCAAACACTCTGCCTCCTTCTTCGTCATCACATGAAATAGAAACTCTGATGGGTGGAGCACAGATATTCGCCAATATCCACAACCCTAA
TCAAAAACATTTAACTTCGTTTGTTAGCATTATGAACTCTGGTACGGAAATTTTAAACAAACTGAAAGCCGATGCTGCAGCGAACATCCCATATCGGCCATATCAGTTCGATGTGGGCAATGTTGTAGAACAAAATACGGAGCTCCTGCGCTTCTTGAAAGAAACAATAGAACCAACTCGCTCTTCAGAAAACATATTGC
CTGAAACTTCACAGCAACAACAGCACAGTCAAGAACCATGTGGAGAAGATGGGGAGAAAGAACAGATTGAGCAACAGTAAACTTGTTCGTAGTTATTTGAAGCCTGG

>TRINITY_DN42837_c0_g1_i13 from sample A8 / Example unspliced isoform:

AGAGAGTCAGCGTTTCGAATTGCCCTTCAGCGCTATAAAACTATCGATCAGGAAATATTCGACACCAAACATGAAGCTGGTCTACCTGCATTCCTCCCAACAGGCTTAACCGTCCAGTTAAATAACTTTGATTCTTGCAGGTGAATACTTTGATTGTCGCTAGAAGCTGGCCTACATTCATTCATTGCAACAGGCTTAAC
CTTCCCGTTAAATAACTTTGATTCTTGCAGATGAATACTTAGATTTGTGAATACTTAGATTCTGGTAAGAAGCTGGCTTACATTCATTCCTTGCGAGTGCCTTAACCTTCCAGTAAATAAGTTTGATTCTTGCATGTGAATACTTTGATTCTCGCAAGGTGAATATGGCAATGAGATTGGCAAAGGACAGGCGTGGATAC
TTTTGGAGAACAGCAGAGGGTTCTCCTTCCCAGATAAATAAGCAAATTTGCGAGGAGCCTGTTTCACCATGGACGCGAGACATTCTTACGAATCTGGATGATTATGAGCTAGCTCGTGATCGCGGTGAATTATATAACAATTTTCAGGGCTGCGGAAGCTGGACAAAGTCGATTAAATCCCCAAACGTTGTGGGATTTAG
TCCTGGGAGTGGGCAATTTGTAGGACATCAGCAGCACAGGGAAGACAATCCCATTATTTTCTCATCGCAACATACTGGTCTTTCCTTCTCTGGCTCTAATCGCTTAAACCCTAATTATTCCAAGAATCCTGGACATGGGATTCCCAGCAGCAGTTTTGTTCTTCTCTCCTCTAGCTCTAATCCCTTAAACCCTAACTATC
CCATTTATGGACATGAGATTCCCGGCAGTAGTTTTGTTCAATCTCTTCGTAATGTCCCAACTTCAATGTTCATGCCTTCCTCATCGTTACAAACTAGTCTTCCCTCCTCTACGTATAATCCCTTAAACCCTAATTATCCCCAGAATCCCATTTATGGACCTGAGATTCCCAGCAGCAATTTTGTTCAATCTCTTCCCAAT
GTCCCAACTTCAATGTTCATGCCTTCCTCTTTGGGTGCACATCCCGGCCCACAATACTTAAGTTCATCAAACACTCTGCCTCCTTCTTCGTCATCACATGAAATAGAAACTCTGATGGGTGGAGCACAGATATTCGCCAATATCCACAACCCTAATCAAAAACATTTAACTTCGTTTGTTAGCATTATGAACTCTGGTAC
GGAAATTTTAAACAAACTGAAAGCCGATGCTGCAGCGAACATCCCATATCGGCCATATCAGTTCGATGTGGGCAATGTTGTAGAACAAAATACGGAGCTCCTGCGCTTCTTGAAAGAAACAATAGAACCAACTCGCTCTTCAGAAAACATATTGCCTGAAACTTCACAGCAACAACAGCACAGTCAAGAACCATGTGGAG
AAGATGGGGAGAAAGAACAGATTGAGCAACAGTAgtaggttattgaagagggattgaaaatcgtttatttagtttgcagaatcgttaaattgaggattatagtctttctttattataatttcacaaataacttgttcagatatattatttatgtgcaaaccctaattaatgttatgtttttccgttcgctagattttatt
tcaatttgttgaaaccctaattactgaacttgttgtgcagAACTTGTTCGTAGTTATTTGAAGCTTGGGCCTGTTGCATTTGATTGGAAGAAGCTGTATCAGGAAAATGGTAAACGCGGCCCTAGGCTGTGCTATTCCATTGGACGACTGTGTATCAGCGTAGGCACGGCGGCTTGGATTTGGCTTGATTGATCATTCTG
GGAACTTTGAAATTGATATTGTGATCTTGATCTCTTTGTCTCATGGTATGTTTATTTCATGCACCAGCGAACAACGAATAAGGATAAGAATCCTATTTTTCTAAATGTAAAGATTTTCAGGTATCATGCTTTTGGAGAAATGAATGTAAAGATTTTCAGGTATCATGCTTTTGGAGTTCAATAAAAGCGAGTTTAATGCT
CTCATAATTCGAA

Intron 3 GTTATTGAAG ... TGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.18	9	2	2
A2	12.5	14	0	2
A3	33.33	8	0	4
A4	40	3	2	2
A5	33.33	10	0	5
A6	39.76	50	6	33
A7	37.86	64	18	39
A8	40	84	19	56
A9	33.83	88	11	45
A10	40.23	52	10	35

>TRINITY_DN42837_c0_g1_i8 from sample A8 / Example spliced isoform

AGAGAGTCAGCGTTTCGAATTGCCCTTCAGCGCTATAAAACTATCGATCAGGAAATATTCGACACCAAACATGAAGCTGGTCTACCTGCATTCCTCCCAACAGGCTTAACCGTCCAGTTAAATAACTTTGATTCTTGCAGGTGAATACTTTGATTGTCGCTAGAAGCTGGCCTACATTCATTCATTGCAACAGGCTTAAC
CTTCCCGTTAAATAACTTTGATTCTTGCAGATGAATACTTAGATTTTGGTAAGAAGCTGGCTTACATTCATTCCTTGCGAGTGCCTTAACCTTCCAGTAAATAAGTTTGATTCTTGCATGTGAATACTTTGATTCTCGCAAGGTGAATATGGCAATGAGATTGGCAAAGGACAGGCGTGGATACTTTTGGAGAACAGCAG
AGGGTTCTCCTTCCCAGATAAATAAGCAAATTTGCGAGGAGCCTGTTTCACCATGGACGCGAGACATTCTTACGAATCTGGATGATTATGAGCTAGCTCGTGATCGCGGTGAATTATATAACAATTTTCAGGGCTGCGGAAGCTGGACAAAGTCGATTAAATCCCCAAACGTTGTGGGATTTAGTCCTGGGAGTGGGCAA
TTTGTAGGACATCAGCAGCACAGGGAAGACAATCCCATTATTTTCTCATCGCAACATACTGGTCTTTCCTTCTCTGGCTCTAATCGCTTAAACCCTAATTATTCCAAGAATCCTGGACATGGGATTCCCAGCAGCAGTTTTGTTCTTCTCTCCTCTAGCTCTAATCCCTTAAACCCTAACTATCCCATTTATGGACATGA
GATTCCCGGCAGTAGTTTTGTTCAATCTCTTCGTAATGTCCCAACTTCAATGTTCATGCCTTCCTCATCGTTACAAACTAGTCTTCCCTCCTCTACGTATAATCCCTTAAACCCTAATTATCCCCAGAATCCCATTTATGGACCTGAGATTCCCAGCAGCAATTTTGTTCAATCTCTTCCCAATGTCCCAACTTCAATGT
TCATGCCTTCCTCTTTGGGTGCACATCCCGGCCCACAATACTTAAGTTCATCAAACACTCTGCCTCCTTCTTCGTCATCACATGAAATAGAAACTCTGATGGGTGGAGCACAGATATTCGCCAATATCCACAACCCTAATCAAAAACATTTAACTTCGTTTGTTAGCATTATGAACTCTGGTACGGAAATTTTAAACAAA
CTGAAAGCCGATGCTGCAGCGAACATCCCATATCGGCCATATCAGTTCGATGTGGGCAATGTTGTAGAACAAAATACGGAGCTCCTGCGCTTCTTGAAAGAAACAATAGAACCAACTCGCTCTTCAAAAAACGTATTGCCTGAAACTTCACAGCAACAACAGCACAGTCAAGAACCATGTGGAGATGGGGAGAAAGAACA
GATTGAGCAACAGTAGTAGGACTTGTTCGTAGTTATTTGAAGCTTGGGCCTGTTGCATTTGATTGGAAGAAGCTGTATCAGGAAAATGGTAAACGCGGCCCTAGGCTGTGCTATTCCATTGGACGACTGTGTATCAGCGTAGGCACGGCGGCTTGGATTTGGCTTGATTGATCATTCTGGGAACTTTGAAATTGATATTG
TGATCTTGATCTCTTTGTCT

>TRINITY_DN42837_c0_g1_i11 from sample A8 / Example unspliced isoform:

AGAGAGTCAGCGTTTCGAATTGCCCTTCAGCGCTATAAAACTATCGATCAGGAAATATTCGACACCAAACATGAAGCTGGTCTACCTGCATTCCTCCCAACAGGCTTAACCGTCCAGTTAAATAACTTTGATTCTTGCAGGTGAATACTTTGATTGTCGCTAGAAGCTGGCCTACATTCATTCATTGCAACAGGCTTAAC
CTTCCCGTTAAATAACTTTGATTCTTGCAGATGAATACTTAGATTTGTGAATACTTAGATTCTGGTAAGAAGCTGGCTTACATTCATTCCTTGCGAGTGCCTTAACCTTCCAGTAAATAAGTTTGATTCTTGCATGTGAATACTTTGATTCTCGCAAGGTGAATATGGCAATGAGATTGGCAAAGGACAGGCGTGGATAC
TTTTGGAGAACAGCAGAGGGTTCTCCTTCCCAGATAAATAAGCAAATTTGCGAGGAGCCTGTTTCACCATGGACGCGAGACATTCTTACGAATCTGGATGATTATGAGCTAGCTCGTGATCGCGGTGAATTATATAACAATTTTCAGGGCTGCGGAAGCTGGACAAAGTCGATTAAATCCCCAAACGTTGTGGGATTTAG
TCCTGGGAGTGGGCAATTTGTAGGACATCAGCAGCACAGGGAAGACAATCCCATTATTTTCTCATCGCAACATACTGGTCTTTCCTTCTCTGGCTCTAATCGCTTAAACCCTAATTATTCCAAGAATCCTGGACATGGGATTCCCAGCAGCAGTTTTGTTCTTCTCTCCTCTAGCTCTAATCCCTTAAACCCTAACTATC
CCATTTATGGACATGAGATTCCCGGCAGTAGTTTTGTTCAATCTCTTCGTAATGTCCCAACTTCAATGTTCATGCCTTCCTCATCGTTACAAACTAGTCTTCCCTCCTCTACGTATAATCCCTTAAACCCTAATTATCCCCAGAATCCCATTTATGGACCTGAGATTCCCAGCAGCAATTTTGTTCAATCTCTTCCCAAT
GTCCCAACTTCAATGTTCATGCCTTCCTCTTTGGGTGCACATCCCGGCCCACAATACTTAAGTTCATCAAACACTCTGCCTCCTTCTTCGTCATCACATGAAATAGAAACTCTGATGGGTGGAGCACAGATATTCGCCAATATCCACAACCCTAATCAAAAACATTTAACTTCGTTTGTTAGCATTATGAACTCTGGTAC
GGAAATTTTAAACAAACTGAAAGCCGATGCTGCAGCGAACATCCCATATCGGCCATATCAGTTCGATGTGGGCAATGTTGTAGAACAAAATACGGAGCTCCTGCGCTTCTTGAAAGAAACAATAGAACCAACTCGCTCTTCAAAAAACGTATTGCCTGAAACTTCACAGCAACAACAGCACAGTCAAGAACCATGTGGAG
ATGGGGAGAAAGAACAGATTGAGCAACAGTAGTAGgttattgaagagggattgaaaatcgtttatttagtttgcagaatcgttaaattgaggattatagtctttctttattataatttcacaaataacttgttcagatatattatttatgtgcaaaccctaattaatgttatgtttttccgttcgctagattttatttca
atttgttgaaaccctaattactgaacttgttgtgcagGACTTGTTCGTAGTTATTTGAAGCTTGGGCCTGTTGCATTTGATTGGAAGAAGCTGTATCAGGAAAATGGTAAACGCGGCCCTAGGCTGTGCTATTCCATTGGACGACTGTGTATCAGCGTAGGCACGGCGGCTTGGATTTGGCTTGATTGATCATTCTGGGA
ACTTTGAAATTGATATTGTGATCTTGATCTCTTTGTCTCATGGTATGTTTATTTCATGCACCAGCGAACAACGAATAAGGATAAGAATCCTATTTTTCTAAATGTAAAGATTTTCAGGTATCATGCTTTTGGAGAAATGAATGTAAAGATTTTCAGGTATCATGCTTTTGGAGTTCAATAAAAGCGAGTTTAATGCTCTC
ATAATTCGAA