sp|P68430|H32_ONOVI ----> Histone H3.2

Intron 1 GTGTGTTTTC ... GGAGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.76	25	82	39
A2	71.19	17	54	30
A3	61.82	42	79	57
A4	73.41	23	78	49
A5	84.13	5	38	15
A6	68.92	336	956	534
A7	65.93	208	508	297
A8	67.86	265	685	434
A9	70.97	206	596	411
A10	70.86	124	414	189

>TRINITY_DN109994_c1_g2_i4 from sample A2 / Example spliced isoform

GAAGAGATGGCTCGGACTAAGCAGACTGCCCGTAAATCAACTGGTGGTAAGGCACCTCGAAAGCAGCTGGCAACCAAGGCAGCCAGAAAGTCTGCACCGGCGACTGGTGGAGTGAAGAAACCACACAGGTTCAGGCCTGGCACAGTTGCCCTTAGGGAAATCCGAAAATACCAGAAGAGCACCGAATTGCTGATAAGAAA
GTTGCCCTTCCAGAGGCTCGTAAGAGAGATCGCCCAGGACTTCAAGACGGACTTGAGATTCCAGTCTTCTGCTGTGGCAGCATTGCAAGAAGCGGCGGAGGCATATCTTGTTGGGCTTTTCGAGGACACCAATCTGTGCGCCATCCACGCCAAGAGGGTTACCATCATGCCAAAAGATATCCAGCTCGCTCGCCGCATTC
GTGGAGAGAGAGCATAGATGCATAGTGTACATATCGCTATCAATGCCAATGGCGAGAGAAGTTAATAACAAATTCTAAAACAACATGGCTTGGCTTGGCTTGGCATGGTATGATATACATACTGTTAAATAGGTTTCTTAGCATCAAATGATCTGGATTTTGAAGACTGTATCGTCACGGTGTTCTATTCTATTTTTATC
AATATGTATATACTATTTTTAAAATACTATTTGTCCTTTTAAGGAATAAAACTACTGTGTTTTTCCATAATTTAAATGAAAATTATATTTGTCAAATGTATCTTGACAGCACTTCATCCTTCTAATAAAAACCCAAAATAATTATACA

>TRINITY_DN109994_c1_g2_i6 from sample A2 / Example unspliced isoform:

GAAGAGATGGCTCGGACTAAGCAGACTGCCCGTAAATCAACTGGTGGTAAGGCACCTCGAAAGCAGCTGGCAACCAAGGCAGCCAGAAAGTCTGCACCGGCGACTGGTGGAGTGAAGAAACCACACAGGTTCAGGCCTGGCACAGTTGCCCTTAGGGAAATCCGAAAATACCAGAAGAGCACCGAATTGCTGATAAGAAA
GTTGCCCTTCCAGAGGCTCGTAAGAGAGATCGCCCAGGACTTCAAGACGGACTTGAGATTCCAGTCTTCTGCTGTGGCAGCATTGCAAGAAGCGGCGGAGGCATATCTTGTTGGGCTTTTCGAGGACACCAATCTGTGCGCCATCCACGCCAAGAGGGTTACCATCATGCCAAAAGATATCCAGCTCGCTCGCCGCATTC
GTGGAGAGAGAGCATAGATGCATAGTGTACATATCGCTATCAATGCCAATGgtgtgttttcacaccaccatctgctgtttcttgaaatttgttctccattagttctccataacccccttctttctttggtgtttattacgtttgtcttttcttgcaaaacgcaataggcgagagattccttacttgctagtcattaatat
atatttcctcctgagagtggagatgcagGCGAGAGAAGTTAATAACAAATTCTAAAACAACATGGCTTGGCTTGGCTTGGCATGGTATGATATACATACTGTTAAATAGGTTTCTTAGCATCAAATGATCTGGATTTTGAAGACTGTATCGTCACGGTGTTCTATTCTATTTTTATCAATATGTATATACTATTTTTAAA
ATACTATTTGTCCTTTTAAGGAATAAAACTACTGTGTTTTTCCATAATTTAAATGAAAATTATATTTGTCAAATGTATCTTGACAGCACTTCATCCTTCTAATAAAAACCCAAAATAATTATACA

Intron 2 GTATGTATCT ... TTATATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	16	21	19
A2	54.93	16	17	22
A3	57.14	9	11	13
A4	57.45	10	20	7
A5	66.67	4	12	4
A6	19.05	17	3	5
A7	46.67	16	14	14
A8	36.59	13	8	7
A9	28.81	21	9	8
A10	39.39	20	9	17

>TRINITY_DN108367_c0_g1_i7 from sample A5 / Example spliced isoform

TAGAAATTTATTTATATTAAACTGTTCATAAAAAATATTTTGATTTTTAATGACATGATGAGAATAAAACAATATTTTGATTAAGAATAAAACAAATAAAACCTGACGAAGAAGGATAAGTTCTGGGCTTTGCTTGAAAATACAATGACGACAAAAGTTTTTCCTTAATATTTACAAAGGTTGAGACAGCAAAATTATAG
GCAATAATTCTTGCAAAATGGTTCACTACAGGATTGGAAGACTACAGTGAGTGATGATATTCTTCTCGATAGCACCAAAGTTAAAAACAGGAATGTGATCAATTATGTCCCTTATGCAATGGGAAGAATGGACAGACTCTGGGGGTGACGATGCTCTGGATTTCAAACCTGAGAAATGGCTCAATAACGATGGCGTCTTC
CAACCTCAATCTCCCTTTAAGTTCACAACGTTTCATGCTGGGCCAAGGATTTGCCTGGGCAAAGACTGCCTACTTGCAAATGAAAATTGCTGCAACAACGTTACTTAGTTTCTTGAAGTTTGAATTGGTCGCAGGCAATCGATTTGAGTACATGGTTGCTTTAACTTTACT

>TRINITY_DN108367_c0_g1_i9 from sample A5 / Example unspliced isoform:

AGGGAGATCGCCCAGGACTTCAAGACGGATTTGAGGTTCCAGTCGTCTGCCGTGTCTGCATTGCAAGAAGCGGCGGAGGCATATCTTGTTGGGCTCTTCGAGGACACCAATCTGTGCGCCATCCACGCCAAAAGGGTTACCATAATGCCCAAAGATATCCAGCTCGCTCGCCGCATCCGTGGAGAGAGGGCATAATCATA
TATATCACTATCAATCCCAATGGATTGGAAGACTACAGTGAGTGATGATATTCTTCTCGATAGCACCAAAGTTAAAAACAGGAATGTGATCAATTATGTCCCTTATGCAATGGGAAGAATGGACAGACTCTGGGGGTGACGATGCTCTGGATTTCAAACCTGAGAAATGGCTCAATAACGATGGCGTCTTCCAACCTCAA
TCTCCCTTTAAGTTCACAACGTTTCATgtatgtatctatgcatctacccatcaattctctgaatctccaaagcagacaataaattttcaaattgtatctatcctgatacgatcaactttatatacagGCTGGGCCAAGGATTTGCCTGGGCAAAGACTGCCTACTTGCAAATGAAAATTGCTGCAACAACGTTACTTAGT
TTCTTGAAGTTTGAATTGGTCGCAGGCAATCGATTTGAGTACATGGTTGCTTTAACTTTACT

Intron 3 GTGTGTTTTC ... CATCCTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.55	14	39	43
A2	55.56	28	31	39
A3	58.68	25	35	36
A4	54.78	26	25	38
A5	76	6	17	21
A6	62.25	419	671	711
A7	67.33	156	299	344
A8	62.18	239	378	408
A9	66.69	252	500	509
A10	66.13	138	277	262

>TRINITY_DN117063_c0_g1_i10 from sample A1 / Example spliced isoform

CGGTGTTTAACATTCTGGATTCAAGAAATTGTAAAGCAAATCAGAAGAGAGGAAAGCAGAAAAGATGGCCCGAACGAAGCAGACAGCCCGTAAATCCACCGGCGGGAAGGCGCCGAGGAAGCAGCTTGCAACAAAAGCTGCAAGAAAATCTGCGCCGGCGACAGGCGGAGTGAAGAAGCCTCACAGGTTTAGGCCGGGCA
CTGTTGCTCTCCGTGAGATCCGAAAGTATCAGAAGAGCACCGAGCTTCTTATAAGGAAGCTGCCCTTCCAGCGTCTTGTCAGAGAAATTGCTCAGGACTTTAAGACGGATTTGAGGTTCCAGTCGTCTGCTGTTGCGGCTCTGCAAGAAGCGGCGGAGGCCTATCTTGTTGGTCTCTTTGAGGATACAAACCTGTGCGCT
ATTCACGCCAAGAGGGTTACTATTATGCCCAAAGACATTCAGCTCGCTCGGCGAATCCGTGGAGAGAGGGCTTAATCGACACAAGTATCAATATCAGTATTATCAATAACATAAATGCAATTCCGTTCCAGGCGATTCCTGTTTTAGGCAGTTACTTCTGCTGTACGTGCTGCAATCATAGCCTTGGTATGAGGTTAAAG
AGTACATCCTGTAGCCTTTGTTTAGCAAATATCAGAGGCTGTTTTTTTATCCTTTGTTTTTGGGTACTGTACTGTGGCTATCCTTATTCTTTGTATATGCCTAAATGGTTGGCATTATTTGTCACTCAAGCAATCTTTTATTTTGTGAAAAAATAATCTTTTAGTTCTTGGTTTAGGAAGTGTGAGGGTTTGAAACAGTG
GATCCATTGGAGATGTTTCATGGCAATTGATGTGTTTTTTTATTTAGTGTGTTAGTGTAGTAAATATGGAGTTTTTATTAATTATGGAATGTTTAAATTGTGATTAAGAATTTATTAATAGATCTAGTATTCTTAAAGACCTTAGAGCATCTATATTTTGGGAATTTAAATGATGTTATTTGGAATTTTAATAATTTTTT
TAGAAAATTTGAATAGATTTGATTGAAATCATGTTAAAAAAGGCATGAGAATGGATGAAATTGTCATGTGCTTGAGAATGGATGAAATTGTTGTGTTTAGGTTATGTGTCAATCAAAATATTGGGCTAGAAATTTGAAGTTTATGCATTCAGTTTTATATAAGTTTATACATTCAGTTTTATACATGAAGTTGCATGAGA
ATGGATGAAATTGTCATGTGTTTGAGAATGGATGAAATTGTCACGTGTTTGAGAATGGATGAAATTGTTGTTTAGATTATGTGTTGATCAAAATATTGGGCTAGAAATTTTAAGGTTATACATTCAATTTTATACATGAAGTTGTCAATAATCAAATTGGTGAGCTAGAAATTTTGAAGTTATATAGCTTTATATATGTA
GTTGCTAGTAGGTATTAGGCAATATCTTTGAAAGCTTGCAATTTTTTTTGATAGATAAATCATTGATACTTATATTTTATTTTATTGGCAATTATTTTAGTGCTTTCTTTTATTCTTATATAAATCAACTAATCCATTTTTCAATAAAAAGAATTTTGGGGAACTCTTTGGACATTATTGTAATTGTGAATAGTATTTAG
TGCAAAATCATTGGTTTTCAAGGACATGGTAT

>TRINITY_DN117063_c0_g1_i3 from sample A1 / Example unspliced isoform:

CGGTGTTTAACATTCTGGATTCAAGAAATTGTAAAGCAAATCAGAAGAGAGGAAAGCAGAAAAGATGGCCCGAACGAAGCAGACAGCCCGTAAATCCACCGGCGGGAAGGCGCCGAGGAAGCAGCTTGCAACAAAAGCTGCAAGAAAATCTGCGCCGGCGACAGGCGGAGTGAAGAAGCCTCACAGGTTTAGGCCGGGCA
CTGTTGCTCTCCGTGAGATCCGAAAGTATCAGAAGAGCACCGAGCTTCTTATAAGGAAGCTGCCCTTCCAGCGTCTTGTCAGAGAAATTGCTCAGGACTTTAAGACGGATTTGAGGTTCCAGTCGTCTGCTGTTGCGGCTCTGCAAGAAGCGGCGGAGGCCTATCTTGTTGGTCTCTTTGAGGATACAAACCTGTGCGCT
ATTCACGCCAAGAGGGTTACTATTATGCCCAAAGACATTCAGCTCGCTCGGCGAATCCGTGGAGAGAGGGCTTAATCGACACAAGTATCAATATCAGTATTATCAATAACATAAATGgtgtgttttcacaccactatgctttttctcagatttgttcatccttgatttctacttcttgatctttgagtttggagagttaa
gttgattgagttcctttcttttacatcctttagCAATTCCGTTCCAGGCGATTCCTGTTTTAGGCAGTTACTTCTGCTGTACGTGCTGCAATCATAGCCTTGGTATGAGGTTAAAGAGTACATCCTGTAGCCTTTGTTTAGCAAATATCAGAGGCTGTTTTTTTATCCTTTGTTTTTGGGTACTGTACTGTGGCTATCCT
TATTCTTTGTATATGCCTAAATGGTTGGCATTATTTGTCACTCAAGCAATCTTTTATTTTGTGAAAAAATAATCTTTTAGTTCTTGGTTTAGGAAGTGTGAGGGTTTGAAACAGTGGATCCATTGGAGATGTTTCATGGCAATTGATGTGTTTTTTTATTTAGTGTGTTAGTGTAGTAAATATGGAGTTTTTATTAATTA
TGGAATGTTTAAATTGTGATTAAGAATTTATTAATAGATCTAGTATTCTTAAAGACCTTAGAGCATCTATATTTTGGGAATTTAAATGATGTTATTTGGAATTTTAATAATTTTTTTAGAAAATTTGAATAGATTTGATTGAAATCATGTTAAAAAAGGCATGAGAATGGATGAAATTGTCATGTGCTTGAGAATGGATG
AAATTGTTGTGTTTAGGTTATGTGTCAATCAAAATATTGGGCTAGAAATTTGAAGTTTATGCATTCAGTTTTATATAAGTTTATACATTCAGTTTTATACATGAAGTTGCATGAGAATGGATGAAATTGTCATGTGTTTGAGAATGGATGAAATTGTCACGTGTTTGAGAATGGATGAAATTGTTGTTTAGATTATGTGT
TGATCAAAATATTGGGCTAGAAATTTTAAGGTTATACATTCAATTTTATACATGAAGTTGTCAATAATCAAATTGGTGAGCTAGAAATTTTGAAGTTATATAGCTTTATATATGTAGTTGCTAGTAGGTATTAGGCAATATCTTTGAAAGCTTGCAATTTTTTTTGATAGATAAATCATTGATACTTATATTTTATTTTA
TTGGCAATTATTTTAGTGCTTTCTTTTATTCTTATATAAATCAACTAATCCATTTTTCAATAAAAAGAATTTTGGGGAACTCTTTGGACATTATTGTAATTGTGAATAGTATTTAGTGCAAAATCATTGGTTTTCAAGGACATGGTAT

Intron 4 GTGTGTTTTC ... GGAGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.68	19.7	71.7	32
A2	71.41	15	49.3	25
A3	64.39	34.7	69	50
A4	78.06	17.7	70.7	42.3
A5	86.75	4	37.3	13
A6	71.23	287.7	863	518
A7	68.88	176.7	459.7	297
A8	70.42	226	613.7	435
A9	72.44	181.7	536.7	401
A10	73.14	103.7	367	180

>TRINITY_DN48977_c4_g4_i1 from sample A7 / Example spliced isoform

GCTGTGGCAGCATTGCAAGAAGCGGCGGAGGCATATCTTGTTGGGCTCTTCGAGGACACCAATCTGTGCGCCATCCACGCCAAGAGGGTTACCATCATGCCAAAAGATATCCAGCTCGCTCGCCGCATTCGTGGAGAGAGAGCATAGATGCATAGTGTACATATCGCTATCAATGCCAATGGCGAGAGAAGTTAATAACA
AATTCTAAAACAACATGGCTTGGCTTGGCTTGGCATGGTATGATATACATACTGTTAAATAGGTTTCTTAGCATCAAATGATCTGGATTTTGAAGACTGTATCGTCACGGTGTTCTATTCTATTTTTATCAATATGTATATACTATTTTTAAAATACTATTTGTCCTTTTAAGGAATAAAACTACTGTGTTTTTCCATAA
TTTAAATGAAAATTATATTTGTCAAATGTATCTTGACAGCACTTCATCCTTCT

>TRINITY_DN48977_c4_g4_i2 from sample A7 / Example unspliced isoform:

GCTGTGGCAGCATTGCAAGAAGCGGCGGAGGCATATCTTGTTGGGCTCTTCGAGGACACCAATCTGTGCGCCATCCACGCCAAGAGGGTTACCATCATGCCAAAAGATATCCAGCTCGCTCGCCGCATTCGTGGAGAGAGAGCATAGATGCATAGTGTACATATCGCTATCAATGCCAATGgtgtgttttcacaccacca
tctgctgtttcttgaaatttgttctccataacccccttctttctttggtgtttattacgtttgtcttttcttgcaaaacgccataggcgagagattccttacttgctagtcattaatatatatttcctcctgagagtggagatgcagGCGAGAGAAGTTAATAACAAATTCTAAAACAACATGGCTTGGCTTGGCTTGGC
ATGGTATGATATACATACTGTTAAATAGGTTTCTTAGCATCAAATGATCTGGATTTTGAAGACTGTATCGTCACGGTGTTCTATTCTATTTTTATCAATATGTATATACTATTTTTAAAATACTATTTGTCCTTTTAAGGAATAAAACTACTGTGTTTTTCCATAATTTAAATGAAAATTATATTTGTCAAATGTATCTT
GACAGCACTTCATCCTTCT