NA ----> NA

Intron 1 GTTACCGCAT ... TGTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.11	11	17	6
A2	42.86	12	12	6
A3	42.86	8	7	5
A4	68.42	3	10	3
A5	0	5	0	0
A6	48.15	21	23	16
A7	32.39	72	55	14
A8	35.71	63	52	18
A9	34.45	78	61	21
A10	30.61	51	39	6

>TRINITY_DN45228_c1_g1_i13 from sample A6 / Example spliced isoform

GGAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACGCCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATA
TATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCGTATGCAAACATCTAGATGCTTCCCATCGATTCACTTGATTACTTCTTCAGAGGATAAATTCACCCATTATCAAAATGCTATCGTGGGACGTTACGATGGCATCGGATTATAAA
CAATTTACCAAGTTGTTGTTTCTCAAAACGCAGATCGCTGGTAGGAGATAAAGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAAGGGATTTCAAATTAAGATGCTGAGAAATAATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGAGAAGACAAGGAAGAT
TAACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAATCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGAGAGGGTCTTGACAGAAGAAAGAAACCTTGTTTGCTTGATTCTGTTATCTATTTTGTAATCTTTCTGTTTGCAAAGAG

>TRINITY_DN45228_c1_g1_i4 from sample A6 / Example unspliced isoform:

GTATCGTGGGACGATGGCTGTGTATGCAAACATCTAGATGCTTCCCATCGATTCACTTGATTACTTCTTCAGAGGATAAATTCACCCATTATCAAAATGgttaccgcatttcataaaattacagcattaaggaagtttgatattttacaaaattgatagcaaagaagtgcaaaccaatggtcaattccttaaaattcttt
atcctccttttatgacaactccatatttgcgaaaactctaatattattttctgctgttgtctaatatatgtttgtgatgcagCTATCGTGGGACGTTACGATGGCATCGGATTATAAACAATTTACCAAGTTGTTGTTTCTCAAAACGCAGATCGCTGGTAGGAGATAAAGAGTTGAGTTACCAGGTCAACAAGAAATAC
CAGAAAA

Intron 2 GTTACCGCAT ... TGTAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.64	9.5	26.5	6.5
A2	80.28	7	26	10
A3	70.68	7.5	16	8
A4	80.56	3.5	12	8
A5	38.34	3	0.5	2
A6	69.83	20	32.5	33
A7	67.25	68.5	104	60.5
A8	70.69	42	83	36
A9	67.53	72.5	106.5	69
A10	68.55	47	77.5	35

>TRINITY_DN45228_c1_g1_i7 from sample A6 / Example spliced isoform

GGAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACGCCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATA
TATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCGTATGCAAACATCTAGATGCTTCCCGTCGATTCGTTTGATTACTTCTTCAGAGGATAAATTCACCTATTATCAAAATGGTATCGTGAGACTTTAGGATGGCATCCAATTATAAA
CAATTTACCAGGTCGTTGTTTCTCAAAACGCAGAATCGCTGGCAGGAGATAAAAGGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAAAGGATTTAAAGTTAAGATGCTGAGAAATAATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGAGAAGACAAGGAA
GATTAACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAATCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGAGAGGGTCTTGACAGAAGAAAGAAACCTTGTTTGCTTGATTCTGTTATCTATTTTGTAATCTTTCTGTTTGCAAAGAGGG
AGGAGACACTGCCGTGAAGAGGGGGAAGCTCTGTAAAGAAAGATGGGAGTTGTTTCCCAGAAGCTCTACTCTCTTTCTCCTCAGGGTTCGATTT

>TRINITY_DN45228_c1_g1_i8 from sample A6 / Example unspliced isoform:

GGAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACGCCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATA
TATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCGTATGCAAACATCTAGATGCTTCCCGTCGATTCGTTTGATTACTTCTTCAGAGGATAAATTCACCTATTATCAAAATGgttaccgcatttcataaaattacagcattaaggaag
tttgatattttacaaaattgatagcaaagaagtgcaaaccaatggtcaattccttaaaattctttatcctccattctcgctttactttttatgacaactccatatttgcgaaaactctaatattatttgctgttgttgtctaatatatgtttgtaatgcagGTATCGTGAGACTTTAGGATGGCATCCAATTATAAACAA
TTTACCAGGTCGTTGTTTCTCAAAACGCAGAATCGCTGGCAGGAGATAAAAGGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAA

Intron 3 GTCAATTCCT ... TGTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.39	8	9	6
A2	35.48	10	5	6
A3	45.45	6	5	5
A4	26.67	11	5	3
A5	0	5	0	0
A6	19.73	59	13	16
A7	14.39	113	24	14
A8	29.03	66	36	18
A9	20.16	103	31	21
A10	16.23	80	25	6

>TRINITY_DN45228_c1_g1_i6 from sample A6 / Example spliced isoform

GTATCGTGGGACGATGGCTGTGTATGCAAACATCTAGATGCTTCCCATCGATTCACTTGATTACTTCTTCAGAGGATAAATTCACCCATTATCAAAATGGTTACCGCATTTCATAAAATTACAGCATTAAGGAAGTTTGATATTTTACAAAATTGATAGCAAAGAAGTGCAAACCAATGCTATCGTGGGACGTTACGATG
GCATCGGATTATAAACAATTTACCAAGTTGTTGTTTCTCAAAACGCAGATCGCTGGTAGGAGATAAAGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAAGGGATTTCAAATTAAGATGCTGAGAAATAATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGA
GAAGACAAGGAAGATTAACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAATCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGAGAGGGTCTTGACAGAAGAAAGAAACCTTGTTTGCTTGATTCTGTTATCTATTTTGTAATCTTTCTGT
TTGCAAAGAG

>TRINITY_DN45228_c1_g1_i4 from sample A6 / Example unspliced isoform:

GTATCGTGGGACGATGGCTGTGTATGCAAACATCTAGATGCTTCCCATCGATTCACTTGATTACTTCTTCAGAGGATAAATTCACCCATTATCAAAATGGTTACCGCATTTCATAAAATTACAGCATTAAGGAAGTTTGATATTTTACAAAATTGATAGCAAAGAAGTGCAAACCAATGgtcaattccttaaaattcttt
atcctccttttatgacaactccatatttgcgaaaactctaatattattttctgctgttgtctaatatatgtttgtgatgcagCTATCGTGGGACGTTACGATGGCATCGGATTATAAACAATTTACCAAGTTGTTGTTTCTCAAAACGCAGATCGCTGGTAGGAGATAAAGAGTTGAGTTACCAGGTCAACAAGAAATAC
CAGAAAA

Intron 4 GTCAATTCCT ... TGTAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.09	9.9	9	6.9
A2	39.57	12.6	5.7	10.7
A3	42.86	10	7	8
A4	31.71	14	5	8
A5	18.24	6	0	2.7
A6	25.99	66.7	13.9	33
A7	23.57	143.9	26.7	62
A8	33.33	71.6	35.1	36.4
A9	27.57	132.3	32	68.7
A10	22.35	101	22.6	35.6

>TRINITY_DN113641_c1_g1_i5 from sample A1 / Example spliced isoform

GAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACACCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATAT
ATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCGTATGCAAACATCTAGATGCTTCCCGTCGATTCGTTTGATTACTTCTTCAGAGGATAAATTCACCCATTATCAAAATGGTTACTGCATTTCATAAAATTACAGCATTAAGGAAGT
TTGATATTTTACAAAATTGATAGCAAAGAAGTGCAAACCAATGGTATCGTGAGACTTTAGGATGGCATCCAATTATAAACAATTTACCAGGTCGTTGTTTCTCAAAACGCAGAATCGCTGGCAGGAGATAAAAGGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAAAGGATTTAAAGTTAAGATGCTGAGAAATA
ATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGAGAAGACAAGGAAGATTAACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAATCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGA
GAGGGTCTTGACAGAAGAAAGAAACCTTGTTTTCTTGATTCTGTTATCTATTTTGTAATCTTTCTGTTTGCAAAGAGGGAGGAGACACTGCCGTG

>TRINITY_DN113641_c1_g1_i3 from sample A1 / Example unspliced isoform:

GAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACACCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATAT
ATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCGTATGCAAACATCTAGATGCTTCCCGTCGATTCGTTTGATTACTTCTTCAGAGGATAAATTCACCCATTATCAAAATGGTTACTGCATTTCATAAAATTACAGCATTAAGGAAGT
TTGATATTTTACAAAATTGATAGCAAAGAAGTGCAAACCAATGgtcaattccttaaaattctttatcctcctttctcgctttactttttatgacaactccatatttgcgaaaactctaatattatttgctgttgttgtctaatatatgtttgtaatgcagGTATCGTGAGACTTTACGATGGCATCCAATTATAAACAAT
TTACCAGGTCGTTGTTTCTCAAAACGCAGAATCGCTGGCAGGAGATAAAAGGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAAAGGATTTAAAGTTAAGATGCTGAGAAATAATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGAGAAGACAAGGAAGATT
AACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAATCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGAGAGGGTCTTGACAGAAGAAAGAAACCTTGTTTTCTTGATTCTGTTATCTATTTTGTAATCTTTCTGTTTGCAAAGAGGGAGGA
GACACTGCCGTG

Intron 5 GTATGCAAAC ... TGTAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	83.2	9	44.6	6.8
A2	70.77	19	46	10.6
A3	61.28	15.8	25	8
A4	59.38	13	19	8
A5	50	3	3	2.6
A6	43.32	62.8	48	33
A7	54.5	171	204.8	61.6
A8	62.74	96.8	163	36.2
A9	57.46	148.8	201	67.8
A10	62.15	101.8	167.2	35.8

>TRINITY_DN113641_c1_g1_i4 from sample A1 / Example spliced isoform

GAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACACCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATAT
ATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCGTATCGTGAGACTTTAGGATGGCATCCAATTATAAACAATTTACCAGGTCGTTGTTTCTCAAAACGCAGAATCGCTGGCAGGAGATAAAAGGAGTTGAGTTACCAGGTCAACAAG
AAATACCAGAAAAAGGATTTAAAGTTAAGATGCTGAGAAATAATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGAGAAGACAAGGAAGATTAACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAA
TCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGAGAGGGTCTTGACAGAAGAAAGAAACCTTGTTTTCTTGATTCTGTTATCTATTTTGTAATCTTTCTGTTTGCAAAGAGGGAGGAGACACTGCCGTG

>TRINITY_DN113641_c1_g1_i3 from sample A1 / Example unspliced isoform:

GAAACATTAACTAAAAGCTCCACGAAGTTGCCAGCAAAATTCTTGAAATTACTATGATCCCACTAAAGATAGAGATTGCTTCCACACCTCTCGATCTGGTTGAAGGAATTAATGATTTCGTTCAAACAGTTTTGTTGCTATATCCACCCATATGTTAAGGAACTTCATGTTATGGCGTCCACATTTTAAGCAGAGAATAT
ATGAGAATGGCCTGCCAGAAATCAAGAATAAAAATACGACACCAGGTCAATGGAGAAAATAAAGATTGGTAGCAAATAAGAACGCgtatgcaaacatctagatgcttcccgtcgattcgtttgattacttcttcagaggataaattcacccattatcaaaatggttactgcatttcataaaattacagcattaaggaagt
ttgatattttacaaaattgatagcaaagaagtgcaaaccaatggtcaattccttaaaattctttatcctcctttctcgctttactttttatgacaactccatatttgcgaaaactctaatattatttgctgttgttgtctaatatatgtttgtaatgcagGTATCGTGAGACTTTACGATGGCATCCAATTATAAACAAT
TTACCAGGTCGTTGTTTCTCAAAACGCAGAATCGCTGGCAGGAGATAAAAGGAGTTGAGTTACCAGGTCAACAAGAAATACCAGAAAAAGGATTTAAAGTTAAGATGCTGAGAAATAATGCGATCATTCTGAGGCGGATCATCTGGCAGAAGTTTGAGAAGCATCACAGCAGTCAAAGAGCTGAGAAGACAAGGAAGATT
AACGGAGATTTCTGTAACAGTCGGCTGATTGTGTGGAGGCGTTTCTGGAGTGTAGTTATATGCTTGAAATAGAAATCATCTATCCTATAAAGAGAGCAGTGAATCGATTGTCCAAGAGAGGGTCTTGACAGAAGAAAGAAACCTTGTTTTCTTGATTCTGTTATCTATTTTGTAATCTTTCTGTTTGCAAAGAGGGAGGA
GACACTGCCGTG