sp|Q9M612|NACA_PINTA ----> Nascent polypeptide-associated complex subunit alpha-like protein

Intron 1 GTGGCATTCT ... CTTATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.7	293	97	56
A2	19.13	389	99	85
A3	17.93	451	110	87
A4	20	264	60	72
A5	17.2	207	52	34
A6	18.05	2699	664	525
A7	19.41	3077	815	666.7
A8	19.05	2205	551	487
A9	17.49	3017	734	545
A10	18.14	2414	622	448

>TRINITY_DN40215_c1_g1_i6 from sample A6 / Example spliced isoform

CTTGGAGGAATCTTCGACGAGTTTCCCTAACCCAAAATGTTCAATGAATGAAACAACTTATGTGCTCCTCCGTGCAAATAAATATTACTAGGGTTTATGATACATAGCTTTATAGTTAAACCCATATCATTATTTTCTTCCTTTTATCGTTATTTTCTTCTTTCTGTGGATGCTCTACTGCTATTCCGTGGTTTTTGTGA
TTAGGGGAGGAGCAACATGCCAGCCCAGATAGAGGAGGAGCAGCTCGCTGCTAAGCTGGAGGAGCAAAAGCTCCAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGAGACCAAGGTGCTGATGGAAGTGGGAAGTCAA
AGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATATTCTGTTTGTTATTTCAAATCCCGATGTCTTCAAAAGTCCTGCTTCGGATACTTACATAGTGTTTGGTGAAGCTAAGATTGAGGACTTGAGTTCTCAG
TTGCAGACACAAGCAGCTGAGCAGTTCAAGGCACCTGATATGAGTCATGTAATGCTCAAGCCAGAATCGTCTGCAGCTGCACAGGAGGAAGATGAGGAAGTAGATGACACTGGAGTGGAGCCCAAAGACATTGAACTGGTAATGACCCAAGCTGGAGTTTCAAGAGTTAAGGCAGTTAAAGCTCTAAAAGCTGCTAGTGG
AGATATTGTTAGTGCGATCATGGACCTTACAACATAGGTGGAGGAACTGATATAGCCAGCAAGTATTTGAAAATAATGTCCTTTAATTAATGATTTCATAAGATGTTTGGTCATGTAAGTTGATTCTTAAATGTTTTGGGTGACAGATTGTAACTTTTGGTACCATCATTGTTAGAAAACAGTTTATCATAATGTCTGTT
TCTGTTCTCTGGAACTATTTTTTTCAGAGATTAGATACAATGGTTTTGAGATTGTGGTCTTCTTTCCTCCCTATTTCGTAAGTGTTTTTACATGCTACTGTCCTACAGGGATGTCCAGGCAAAACTTGGCATTCCATACCTCTGTTCTTGTGTTTTACAAGGGTTTTATGCAGTACACGATATTGTATTTAGATCTAAGT
TGCCAGGAAACTTGGGTACGGG

>TRINITY_DN40215_c1_g1_i3 from sample A6 / Example unspliced isoform:

CTTGGAGGAATCTTCGACGAGTTTCCCTAACCCAAAATGTTCAATGAATGAAACAACTTATGTGCTCCTCCGTGCAAATAAATATTACTAGGGTTTATGATACATAGCTTTATAGTTAAACCCATATCATTATTTTCTTCTTTCTGTGGATGCTCTACTGCTATTCCGTGGTTTTTGTGATTAGGGGAGGAGCAACATGC
CAGCCCAGATAGAGGAGGAGCAGCTCGCTGCTAAGCTGGAGGAGCAAAAGCTCCAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGgtggcattcttcctattctactttcttattttagGAGACCAAGGTGCTGATGG
AAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATATTCTGTTTGTTATTTCAAATCCCGATGTCTTCAAAAGTCCTGCTTCGGATACTTACATAGTGTTTGGTGAAGCTAAGATTGAGG
ACTTGAGTTCTCAGTTGCAGACACAAGCAGCTGAGCAGTTCAAGGCACCTGATATGAGTCATGTAATGCTCAAGCCAGAATCGTCTGCAGCTGCACAGGAGGAAGATGAGGAAGTAGATGACACTGGAGTGGAGCCCAAAGACATTGAACTGGTAATGACCCAAGCTGGAGTTTCAAGAGTTAAGGCAGTTAAAGCTCTA
AAAGCTGCTAGTGGAGATATTGTTAGTGCGATCATGGACCTTACAACATAGGTGGAGGAACTGATATAGCCAGCAAGTATTTGAAAATAATGTCCTTTAATTAATGATTTCATAAGATGTTTGGTCATGTAAGTTGATTCTTAAATGTTTTGGGTGACAGATTGTAACTTTTGGTACCATCATTGTTAGAAAACAGTTTA
TCATAATGTCTGTTTCTGTTCTCTGGAACTATTTTTTTCAGAGATTAGATACAATGGTTTTGAGATTGTGGTCTTCTTTCCTCCCTATTTCGTAAGTGTTTTTACATGCTACTGTCCTACAGGGATGTCCAGGCAAAACTTGGCATTCCATACCTCTGTTCTTGTGTTTTACAAGGGTTTTATGCAGTACACGATATTGT
ATTTAGATCTAAGTTGCCAGGAAACTTGGGTACGGG

Intron 2 GTCTGCGATG ... TTGCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.67	56	5	49
A2	47.53	85	6	77
A3	47.9	87	6	80
A4	49.3	72	6	70
A5	51.43	34	6	36
A6	48.88	525	10	502
A7	48.97	668	31	641
A8	47.97	487	21	449
A9	49.63	545	3	537
A10	48.74	448	16	426

>TRINITY_DN42090_c0_g2_i6 from sample A7 / Example spliced isoform

ATCTTGGAGGAATCTTCGACGAGTTTCCCTAACCCAAAATGTTCAATGAATGAAACAACTTATGTGCTCCTCCGTGCAAATAAATATTACTAGGGTTTATGATACATAGCTTTATAGTTAAACCCATATCATTATTTTCTTCTTTCTGTGGATGCTCTACTGCTATTCCGTGGTTTTTGTGATCAGGGGAGGAGCAACAT
GCCAGCCCAGATAGAGGAGGAGCAGCTCGCTGCTAAGCTGGAGGAGCAAAAGCTCCAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGTGGCATTCTTCCTATTCTACTTTCTTATTTTAGGAGACCAAGGTGCTGAT
GGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAAT

>TRINITY_DN42090_c0_g2_i3 from sample A7 / Example unspliced isoform:

ATTGTTTTAGGATGGATTTTCCAGCAGCCATTCCGTTATTGGTCCAGAAGATGACCTATTCATTAGATCTCAAGGCGTGCCTTTGATTTGATTCTTTAGGTTATTTGCGTATTGTCTGACTTTATGAATACTGTATGGCATCAATCCCAACAAGTGCACACCTTAAGCTGTAACTAGCACATACCCATCTAAACCCGGGT
CTGGTATGTACCTGTAGAGATTGCGAGGGTCCAGAACGATATCTTGAATCCCAATGTTTATTAGACAGGTCCACCATTCTTTCAAATTTGAATATACAATCTGACCTGTTCAACTTCGAGGTTCAGATGAAACCCAGGTTTAGATGGGTTTGTGCTATCGTCCATATTCAATGCATCGTTTAGATTCAAATCTCCAAATA
CACATAATGTTACAGAAAGTGTTATACACATAATGTTACGCACTGTTGATAAACTTCCCCGCCCCACCAGAGCTGTAGAGCATCTTCTCTTCATAGTGCAACTAGGCTCCGTAGGGAGTTTTTTTCCCTATTCATCCAAACTTTCTTAAGAATCAGATAAACTAGAAACAGTTTTCAGAGAAGAGGCCGTGATTCATTTC
ATAGATTTGCTTCTAATGTTGCTGCATTGTAATAATCATATCCATCATCCCTCTTAATGCAAGTTGTATTGGTTTCAATGCCTTTAATTTGGAGATTTGAATGATGAAACTGAAATCGATTCATGTTGGTTTTATCAATGAAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGAC
GAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGTGGCATTCTTCCTATTCTACTTTCTTATTTTAGgtctgcgatggaatatctttcttaataattaagctttatcattttcttttctgaattgatgctgcatatagaacctttcaaacatggctaacatataatccgggtctctgcctgccggggttctaatcttgg
ccattaatgtggtgacaccctgtcttaaccactagacaacagcccaaagggcaactgtttatttgttgcaatctttgttaaaattgacgttttgatcctcaaattaaggagaaccttaccttattcaaataatggcacagtgaagtgattcttgcctatatattcatccattttgttatattctgagcttgcttgcagGA
GACCAAGGTGCTGATGGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATATTCTGTTTGTTATTTCAAATCCTGATGTCTTCAAAAGTCCTGCTTCGGATACTTACATAGTGTTTGG
TGAAGCTAAGATTGAGGACTTGAGTTCTCAGTTGCAGACACAAGCAGCTGAGCAGTTCAAGGCACCTGATATGAGTCATGTAATGCTCAAGCCAGAATCGTCTGCAGCTGCACAGGAGGAAGATGAGGAAGTAGATGACACTGGAGTGGAGCCCAAAGACATTGAACTGGTAATGACCCAAGCTGGAGTTTCAAGAGTTA
AGGCAGTTAAAGCTCTAAAAGCTGCTAGTGGAGATATTGTTAGTGCGATCATGGACCTTACAACATAGGTGGAGGAACTGATATAGCCAGCAAGTATTTGAAAATAATGTCCTTTAATTAATGATTTCATAAGATGTTTGGTCATGTAAGTTGATTCTTAAATGTTTTGGGTGACAGATTGTAACTTTTGGTACCATCAT
TGTTAGAAAACAGTTTATCATAATGTCTGTTTCTGTTCTCTGGAACTATTTTTTTCAGAGATTAGATACAATGGTTTTGAGATTGTGGTCTTCTTTCCTCCCTATTTCGTAAGTGTTTTTACATGCTACTGTCCTACAGGGATGTCCAGGCAAAACTTGGCATTCCATACCTCTGTTCTTGTGTTTTACAAGGGTTTTAT
GCAGTACACGATATTGTATTTAGATCTAAGTTGCCAGGAAACTTGGGTACGG

Intron 3 GTAATAATCA ... ATCAATGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	3	9
A2	100	0	2	5
A3	33.33	2	0	2
A4	57.14	3	5	3
A5	100	0	0	2
A6	81.4	4	14	21
A7	57.89	12	15	18
A8	76.92	3	6	14
A9	86.67	1	5	8
A10	70.59	5	12	12

>TRINITY_DN42090_c0_g2_i4 from sample A7 / Example spliced isoform

ATTGTTTTAGGATGGATTTTCCAGCAGCCATTCCGTTATTGGTCCAGAAGATGACCTATTCATTAGATCTCAAGGCGTGCCTTTGATTTGATTCTTTAGGTTATTTGCGTATTGTCTGACTTTATGAATACTGTATGGCATCAATCCCAACAAGTGCACACCTTAAGCTGTAACTAGCACATACCCATCTAAACCCGGGT
CTGGTATGTACCTGTAGAGATTGCGAGGGTCCAGAACGATATCTTGAATCCCAATGTTTATTAGACAGGTCCACCATTCTTTCAAATTTGAATATACAATCTGACCTGTTCAACTTCGAGGTTCAGATGAAACCCAGGTTTAGATGGGTTTGTGCTATCGTCCATATTCAATGCATCGTTTAGATTCAAATCTCCAAATA
CACATAATGTTACAGAAAGTGTTATACACATAATGTTACGCACTGTTGATAAACTTCCCCGCCCCACCAGAGCTGTAGAGCATCTTCTCTTCATAGTGCAACTAGGCTCCGTAGGGAGTTTTTTTCCCTATTCATCCAAACTTTCTTAAGAATCAGATAAACTAGAAACAGTTTTCAGAGAAGAGGCCGTGATTCATTTC
ATAGATTTGCTTCTAATGTTGCTGCATTTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGG

>TRINITY_DN42090_c0_g2_i1 from sample A7 / Example unspliced isoform:

ATTGTTTTAGGATGGATTTTCCAGCAGCCATTCCGTTATTGGTCCAGAAGATGACCTATTCATTAGATCTCAAGGCGTGCCTTTGATTTGATTCTTTAGGTTATTTGCGTATTGTCTGACTTTATGAATACTGTATGGCATCAATCCCAACAAGTGCACACCTTAAGCTGTAACTAGCACATACCCATCTAAACCCGGGT
CTGGTATGTACCTGTAGAGATTGCGAGGGTCCAGAACGATATCTTGAATCCCAATGTTTATTAGACAGGTCCACCATTCTTTCAAATTTGAATATACAATCTGACCTGTTCAACTTCGAGGTTCAGATGAAACCCAGGTTTAGATGGGTTTGTGCTATCGTCCATATTCAATGCATCGTTTAGATTCAAATCTCCAAATA
CACATAATGTTACAGAAAGTGTTATACACATAATGTTACGCACTGTTGATAAACTTCCCCGCCCCACCAGAGCTGTAGAGCATCTTCTCTTCATAGTGCAACTAGGCTCCGTAGGGAGTTTTTTTCCCTATTCATCCAAACTTTCTTAAGAATCAGATAAACTAGAAACAGTTTTCAGAGAAGAGGCCGTGATTCATTTC
ATAGATTTGCTTCTAATGTTGCTGCATTgtaataatcatatccatcatccctcttaatgcaagttgtattggtttcaatgcctttaatttggagatttgaatgatgaaactgaaatcgattcatgttggttttatcaatgaagTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGAC
GAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGTGGCATTCTTCCTATTCTACTTTCTTATTTTAGGTCTGCGATGGAATATCTTTCTTAATAATTAAGCTTTATCATTTTCTTTTCTGAATTGATGCTGCATATAGAACCTTTCAAACATGGCTAACATATAATCCGGGTCTCTGCCTGCCGGGGTTCTAATCTTGG
CCATTAATGTGGTGACACCCTGTCTTAACCACTAGACAACAGCCCAAAGGGCAACTGTTTATTTGTTGCAATCTTTGTTAAAATTGACGTTTTGATCCTCAAATTAAGGAGAACCTTACCTTATTCAAATAATGGCACAGTGAAGTGATTCTTGCCTATATATTCATCCATTTTGTTATATTCTGAGCTTGCTTGCAGGA
GACCAAGGTGCTGATGGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATGTGAGTTCTCTCTTTCTCTAATGTAGATTGAGAACTCAAACCTGGAGGAGTCTTACAAAATAGTCTTT
CATCGTGAACCATCATTAATTATCAATTTTGTTAAACTGGTCTTACATTACCTTGTCGTTGGCTGCAGATTCTG

Intron 4 GTCAGAAAAT ... ATCAATGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	2.19	402.2	15.5	9
A2	1.11	443.3	22	5
A3	0.4	496.7	27.5	2
A4	0.81	366.7	25	3
A5	0.74	266.8	7	2
A6	0.75	2768.2	100	21
A7	0.56	3208.5	82.5	18
A8	0.66	2110	63.5	14
A9	0.24	3337.3	101	8
A10	0.44	2736.7	75.5	12

>TRINITY_DN41718_c1_g5_i3 from sample A8 / Example spliced isoform

GAGGAATCTTCGACGAGTTTCCCTAACCCAAAATGTTCAATGAATGAAACAACTTATGTGCTCCTCCGTGCAAATAAATATTACTAGGGTTTATGATACATAGCTTTATAGTTAAACCCATATCATTATTTTCTTCTTTCTGTGGATGCTCTACTGCTATTCCGTGGTTTTTGTGATCAGGGGAGGAGCAACATGCCAGC
CCAGATAGAGGAGGAGCAGCTCGCTGCTAAGCTGGAGGAGCAAAAGCTCCAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGTGGCATTCTTCCTATTCTACTTTCTTATTTTAGGTCTGCGATGGAATATCTTTCTT
AATAATTAAGCTTTATCATTTTCTTTTCTGAATTGATGCTGCATATAGAACCTTTCAAACATGGCTAACATATAATCCGGGTCTCTGCCTGCCGGGGTTCTAATCTTGGCCATTAATGTGGTGACACCCTGTCTTAACCACTAGACAACAGCCCAAAGGGCAACTGTTTATTTGTTGCAATCTTTGTTAAAATTGACGTT
TTGATCCTCAAATTAAGGAGAACCTTACCTTATTCAAATAATGGCACAGTGAAGTGATTCTTGCCTATATATTCATCCATTTTGTTATATTCTGAGCTTGCTTGCAGGAGACCAAGGTGCTGATGGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCA
TTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATATTCTGTTTGTTATTTCAAATCCTGATGTCTTCAAAAGTCCTGCTTCGGATACTTACATAGTGTTTGGTGAAGCTAAGATTGAGGACTTGAGTTCTCAGTTGCAGACACAAGCAGCTGAGCAGTTCAAGGCACCTGATATGAGTCATGTAATGCTCAAG
CCAGAATCGTCTGCAGCTGCACAGGAGGAAGATGAGGAAGTAGATGACACTGGAGTGGAGCCCAAAGACATTGAACTGGTAATGACCCAAGCTGGAGTTTCAAGAGTTAAGGCAGTTAAAGCTCTAAAAGCTGCTAGTGGAGATATTGTTAGTGCGATCATGGACCTTACAACATAGGTGGAGGAACTGATATAGCCAGC
AAGTATTTGAAAATAATGTCCTTTAATTAATGATTTCATAAGATGTTTGGTCATGTAAGTTGATTCTTAAATGTTTTGGGTGACAGATTGTAACTTTTGGTACCATCATTGTTAGAAAACAGTTTATCATAATGTCTGTTTCTGTTCTCTGGAACTATTTTTTTCAGAGATTAGATACAATGGTTTTGAGATTGTGGTCT
TCTTTCCTCCCTATTTCGTAAGTGTTTTTACATGCTACTGTCCTACAGGGATGTCCAGGCAAAACTTGGCATTCCATACCTCTGTTCTTGTGTTTTACAAGGGTTTTATGCAGTACACGATATTGTATTTAGATCTAAGTTGCCAGGAAACTTGGGTATGGGGACT

>TRINITY_DN41718_c1_g5_i4 from sample A8 / Example unspliced isoform:

GAGGAATCTTCGACGAGTTTCCCTAACCCAAAATGTTCAATGAATGAAACAACTTATGTGCTCCTCCGTGCAAATAAATATTACTAGGGTTTATGATACATAGCTTTATAGTTAAACCCATATCATTATTTTCTTCTTTCTGTGGATGCTCTACTGCTATTCCGTGGTTTTTGTGATCAGGGGAGGAGCAACATGCCAGC
CCAGATAGAGGAGGAGCAGCTCGCTGCTAAGCTGGAGGAGCAAAAGCTCCAGgtcagaaaatttctgatactctttctgcatttccttctcatttgtttttcattttccctctttagtaaaaacccatttcattgttttaggatggattttccagcagccattccgttattggtccagaagatgacctattcattagatc
tcaaggcgtgcctttgatttgattctttaggttatttgcgtattgtctgacttgatgaatactgtatggcatcaatcccaacaagtgcacaccttaagctgtaactagcacatacccatctaaacccgggtctggtatgtacctgtagagattgcgaaggtccagaacgatatcttgaatcccattgtttattagatagg
tccaccattctttcaaacttgaatatacaatctgacttgttcaacttcgaggttcagatgaaacccaggtttagatgggtttgtgctatcgtccatattcaatgcatcgtttagattcaaatctccaaatacacataatgttacagaaagtgttatacacataatgttacgcactgttgataaacttccccgccccacca
gagctgtagagcatcttctcttcatagtgcaactaggctccgtagggagtttttttccctattcatccaaactttcttaagaatcagataaactagaaacagttttcagagaagaggccgtgattcatttcatagatttgcttctaatgttgctgcattgtaataatcatatccatcatccctcttaatgcaagttgtat
tggtttcaatgcctttaatttggagatttgaatgatgaaactgaaatcgattcatgttggttttatcaatgaagTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGTGGCATTCTTCCTATTCTACTTTCTTATTTTAGG
TCTGCGATGGAATATCTTTCTTAATAATTAAGCTTTATCATTTTCTTTTCTGAATTGATGCTGCATATAGAACCTTTCAAACATGGCTAACATATAATCCGGGTCTCTGCCTGCCGGGGTTCTAATCTTGGCCATTAATGTGGTGACACCCTGTCTTAACCACTAGACAACAGCCCAAAGGGCAACTGTTTATTTGTTGC
AATCTTTGTTAAAATTGACGTTTTGATCCTCAAATTAAGGAGAACCTTACCTTATTCAAATAATGGCACAGTGAAGTGATTCTTGCCTATATATTCATCCATTTTGTTATATTCTGAGCTTGCTTGCAGGAGACCAAGGTGCTGATGGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATG
CTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATATTCTGTTTGTTATTTCAAATCCTGATGTCTTCAAAAGTCCTGCTTCGGATACTTACATAGTGTTTGGTGAAGCTAAGATTGAGGACTTGAGTTCTCAGTTGCAGACACAAGCAGCTGAGCAGTTCAAGGCACCTGA
TATGAGTCATGTAATGCTCAAGCCAGAATCGTCTGCAGCTGCACAGGAGGAAGATGAGGAAGTAGATGACACTGGAGTGGAGCCCAAAGACATTGAACTGGTAATGACCCAAGCTGGAGTTTCAAGAGTTAAGGCAGTTAAAGCTCTAAAAGCTGCTAGTGGAGATATTGTTAGTGCGATCATGGACCTTACAACATAGG
TGGAGGAACTGATATAGCCAGCAAGTATTTGAAAATAATGTCCTTTAATTAATGATTTCATAAGATGTTTGGTCATGTAAGTTGATTCTTAAATGTTTTGGGTGACAGATTGTAACTTTTGGTACCATCATTGTTAGAAAACAGTTTATCATAATGTCTGTTTCTGTTCTCTGGAACTATTTTTTTCAGAGATTAGATAC
AATGGTTTTGAGATTGTGGTCTTCTTTCCTCCCTATTTCGTAAGTGTTTTTACATGCTACTGTCCTACAGGGATGTCCAGGCAAAACTTGGCATTCCATACCTCTGTTCTTGTGTTTTACAAGGGTTTTATGCAGTACACGATATTGTATTTAGATCTAAGTTGCCAGGAAACTTGGGTATGGGGACT

Intron 5 GTGGCATTCT ... TTGCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.33	293	22.5	49
A2	16.52	389	21.8	77
A3	15.07	451	24.5	80
A4	20.96	264	13.6	70
A5	14.81	207	16.1	36
A6	15.68	2699	136.5	502
A7	17.25	3077	169.2	641.3
A8	16.92	2205	115.6	449
A9	15.11	3017	136.8	537
A10	15	2414	120.5	426

>TRINITY_DN42090_c0_g2_i2 from sample A7 / Example spliced isoform

ATCTTGGAGGAATCTTCGACGAGTTTCCCTAACCCAAAATGTTCAATGAATGAAACAACTTATGTGCTCCTCCGTGCAAATAAATATTACTAGGGTTTATGATACATAGCTTTATAGTTAAACCCATATCATTATTTTCTTCTTTCTGTGGATGCTCTACTGCTATTCCGTGGTTTTTGTGATCAGGGGAGGAGCAACAT
GCCAGCCCAGATAGAGGAGGAGCAGCTCGCTGCTAAGCTGGAGGAGCAAAAGCTCCAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGACGAGGACAGCGATGACGAAGAGGGCGATGGTCAAGGAGACCAAGGTGCTGATGGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGA
AAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATATTCTGTTTGTTATTTCAAATCCTGATGTCTTCAAAAGTCCTGCTTCGGATACTTACATAGTGTTTGGTGAAGCTAAGATTGAGGACTTGAGTTCTCAGTTGCAGACACAAGCAGCT
GAGCAGTTCAAGGCACCTGATATGAGTCATGTAATGCTCAAGCCAGAATCGTCTGCAGCTGCACAGGAGGAAGATGAGGAAGTAGATGACACTGGAGTGGAGCCCAAAGACATTGAACTGGTAATGACCCAAGCTGGAGTTTCAAGAGTTAAGGCAGTTAAAGCTCTAAAAGCTGCTAGTGGAGATATTGTTAGTGCGAT
CATGGACCTTACAACATAGGTGGAGGAACTGATATAGCCAGCAAGTATTTGAAAATAATGTCCTTTAATTAATGATTTCATAAGATGTTTGGTCATGTAAGTTGATTCTTAAATGTTTTGGGTGACAGATTGTAACTTTTGGTACCATCATTGTTAGAAAACAGTTTATCATAATGTCTGTTTCTGTTCTCTGGAACTAT
TTTTTTCAGAGATTAGATACAATGGTTTTGAGATTGTGGTCTTCTTTCCTCCCTATTTCGTAAGTGTTTTTACATGCTACTGTCCTACAGGGATGTCCAGGCAAAACTTGGCATTCCATACCTCTGTTCTTGTGTTTTACAAGGGTTTTATGCAGTACACGATATTGTATTTAGATCTAAGTTGCCAGGAAACTTGGGTA
CGG

>TRINITY_DN42090_c0_g2_i1 from sample A7 / Example unspliced isoform:

ATTGTTTTAGGATGGATTTTCCAGCAGCCATTCCGTTATTGGTCCAGAAGATGACCTATTCATTAGATCTCAAGGCGTGCCTTTGATTTGATTCTTTAGGTTATTTGCGTATTGTCTGACTTTATGAATACTGTATGGCATCAATCCCAACAAGTGCACACCTTAAGCTGTAACTAGCACATACCCATCTAAACCCGGGT
CTGGTATGTACCTGTAGAGATTGCGAGGGTCCAGAACGATATCTTGAATCCCAATGTTTATTAGACAGGTCCACCATTCTTTCAAATTTGAATATACAATCTGACCTGTTCAACTTCGAGGTTCAGATGAAACCCAGGTTTAGATGGGTTTGTGCTATCGTCCATATTCAATGCATCGTTTAGATTCAAATCTCCAAATA
CACATAATGTTACAGAAAGTGTTATACACATAATGTTACGCACTGTTGATAAACTTCCCCGCCCCACCAGAGCTGTAGAGCATCTTCTCTTCATAGTGCAACTAGGCTCCGTAGGGAGTTTTTTTCCCTATTCATCCAAACTTTCTTAAGAATCAGATAAACTAGAAACAGTTTTCAGAGAAGAGGCCGTGATTCATTTC
ATAGATTTGCTTCTAATGTTGCTGCATTGTAATAATCATATCCATCATCCCTCTTAATGCAAGTTGTATTGGTTTCAATGCCTTTAATTTGGAGATTTGAATGATGAAACTGAAATCGATTCATGTTGGTTTTATCAATGAAGTCTGATGAGCCAATTATAGAAGATGTTAAGGATGAAGACGATGAAGATGAAGACGAC
GAGGACAGCGATGACGAAGAGGGCGATGGTCAAGgtggcattcttcctattctactttcttattttaggtctgcgatggaatatctttcttaataattaagctttatcattttcttttctgaattgatgctgcatatagaacctttcaaacatggctaacatataatccgggtctctgcctgccggggttctaatcttgg
ccattaatgtggtgacaccctgtcttaaccactagacaacagcccaaagggcaactgtttatttgttgcaatctttgttaaaattgacgttttgatcctcaaattaaggagaaccttaccttattcaaataatggcacagtgaagtgattcttgcctatatattcatccattttgttatattctgagcttgcttgcagGA
GACCAAGGTGCTGATGGAAGTGGGAAGTCAAAGCAAAGTAGGAGTGAGAAAAAGAGCCGAAAAGCAATGCTTAAACTTGGGATGAAACCCATTCCTGGTGTTGCTAGGGTGACCATTAAGAAAAACAAAAATGTGAGTTCTCTCTTTCTCTAATGTAGATTGAGAACTCAAACCTGGAGGAGTCTTACAAAATAGTCTTT
CATCGTGAACCATCATTAATTATCAATTTTGTTAAACTGGTCTTACATTACCTTGTCGTTGGCTGCAGATTCTG