sp|Q9SR15|SYWC_ARATH ----> Tryptophan--tRNA ligase, cytoplasmic

Intron 1 GTACCATTCA ... ATTGTGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.81	96	8.7	30
A2	20.47	101	9	26
A3	25.49	76	12.3	26
A4	26.45	114	14.3	41
A5	9.38	29	2.7	3
A6	16.44	61	3.3	12
A7	19.01	196	24.7	46
A8	25.32	118	14.3	40
A9	20.28	169	16.3	43
A10	14.09	128	11.7	21

>TRINITY_DN98163_c0_g1_i4 from sample A4 / Example spliced isoform

AAAAGATACGAGCGAGCAAACAGCATGTATTGACCGGAAGACCGTGATATATTGTGTCACATGTGGTGATGATAAAGGATTTCAGAAGAGTCCAACCCTCCTTTCATTCTCTCCAGTATCACTACCACTTCCATTCTCTGCAGTTTCACTACCACGTTTATAGGGAATTCAACCAAGAGGCGGACCTCCTGTCAAAAATA
AGCGTTGAATTATCCAAAAGGGGAGGGGAAGATGGGAGGGAGCCATGAGAATGAGAATAAGAATGCAGAGGATGAAAACCAGATCGTGACTCCATGGGAAGTAGCTGCGAAGGAGGGAGGGCGTATTGATTATGACAAGCTCATTGTGCAATTCGGCTGTCAAAAACTAGATCAATCCATTATTCAAAGGGTGGAAAAAC
TCACTGGCAAGCCTGCCCATGTCTTCCTCCGCCGTGGTGTTTTCTTCGCTCACAGGGAATTTGACAAGATTCTTGATGCATTTGAGAAAGGAGAAAAGTTTTATTTATATACTGGCAGAGGACCTTCATCTGAAGCTTTGCATCTGGGACATCTAGTGCCATTCATGTTTACAAAATATTTGCAAGATGCATTCAAAGTA
CCACTTGTAATACAACTCACCGATGATGAGAAATGCATGTGGAAGAATCTTACTGTTGAAGAAAGTCAGAGACTTGCCCGTGAAAATGCCAAAGATATAATTGCATGTGGTTTTGATATTTCAAGGACATTCATATTTTCTGATTTTGACTATGTTGGGGGTGCCTTCTACAAGAACATGGTTAGAATTGGCAAATGTGT
CACCTTGAATCAGGTAAGAGCAATATTTGGCTTCACTGGGGAGGACCACATTGGAAAAGTCAGTTTTCCACCTGTACAAGCGGCTCCATCATTTCCATCTTCATTCCCTCATTTGTTTGGTGACAAAAATAATCTTCGGTGCTTGATTCCATGTGCAATAGATCAGGATCCTTATTTTAGGATGACACGAGATGTTGCAC
CAAGAATAGGATGTCAAAAACCTTCTCTTATTGAATCCCTGTTTTTCCCAGCGCTTCAGGGAGAGACAGGAAAAATGTCTGCAAGTGATTCCACCACAGCAATATATGTAACTGATTCTGCCAAGGACATCAAGAACAAGGTGAATAAATATGCCTTCTCTGGAGGTGGAGATACAATAGAGAATCATAGGAAATTCGGG
GCGAATCTTGAGGTGGATATATCGGTCAAGTATCTTAGCTTCTTTCTTGAGGATGATGCAGAATTGGAAAACATTAAACAAGAATATGGAGCTGGAAGAATGCTCACTGGAGAGGTTAAAAAGCTGCTTATTGAAGTTTTGACAGACATGGTTGAACGGCATCGTAAAGCTCGAGCTGCAGTAACAGATGAGATGGTCGA
TGTATTTATGGCTGCAAGGCCTCTTCCTGGTATGTTTGGTTAAGGACGTTTCTCTTTCGACCATTTTGTTAGAGAATACAGAGTTTCACTTGGCATATCTAAGTTCCAATTTTTTTCCGAGTTGTCTCTTCATTTCATGCTGTGAAATTAAAGTTGTTAGGACAAAGTTAAAGCCATTGAGTTAAATTTCCCATTGTAAG
AAAATCACTTTCGTCAAAAGGTTATTTGCAAAATTAGACTGCAAGCACTTTGAATTGGTCAGAGATCAATTCAACCAGTCAAAATTGTACAGTATTCGTAATTGCCATATTGACATGGATGGGAGAATCTCAATAAAAGTTTGACTATGTGCAGTCACTTTGCAAAATTGTACAGTATTCGTAATTGCCATATTGACATG
GATGGGAGAATCTCAATAAAAGTTTGACTATGTGCAGTCACTTTGCAATCAGAACATGTTGTAGCAATTTATGCCGCCGACATAGATTAAGATTTATACTAATTTTCTCAGGAGAGTCCATTCTCGTTGCA

>TRINITY_DN98163_c0_g1_i3 from sample A4 / Example unspliced isoform:

TAAGCGTTGAATTATCCAAAAGgtaccattcattacgaagagtggagggactctgttcttatctcgaatggctctatatcctttttccatttccagtcttgttgctcttcttcatttctgtgcgtagcatcttttgggatcatctttatttcgggattggcaagggtctgtttttgccattctccttgtatttggtttcg
ttaggcgtgcgcaggcacggcccataggttattgctggaactgcttggcccattatcgtcgactgtagttgtccatagttcattgtattgtggaagGGGAGGGGAAGATGGGAGGGAGCCATGAGAATGAGAATAAGAATGCAGAGGATGAAAACCAGATCGTGACTCCATGGGAAGTAGCTGCGAAGGAGGGAGGGCGT
ATTGATTATGACAAGCTCATTGTGCAATTCGGCTGTCAAAAACTAGATCAATCCATTATTCAAAGGGTGGAAAAACTCACTGGCAAGCCTGCCCATGTCTTCCTCCGCCGTGGTGTTTTCTTCGCTCACAGGGAATTTGACAAGATTCTTGATGCATTTGAGAAAGGAGAAAAGTTTTATTTATATACTGGCAGAGGACC
TTCATCTGAAGCTTTGCATCTGGGACATCTAGTGCCATTCATGTTTACAAAATATTTGCAAGATGCATTCAAAGTACCACTTGTAATACAACTCACCGATGATGAGAAATGCATGTGGAAGAATCTTACTGTTGAAGAAAGTCAGAGACTTGCCCGTGAAAATGCCAAAGATATAATTGCATGTGGTTTTGATATTTCAA
GGACATTCATATTTTCTGATTTTGACTATGTTGGGGGTGCCTTCTACAAGAACATGGTTAGAATTGGCAAATGTGTCACCTTGAATCAGGTAAGAGCAATATTTGGCTTCACTGGGGAGGACCACATTGGAAAAGTCAGTTTTCCACCTGTACAAGCGGCTCCATCATTTCCATCTTCATTCCCTCATTTGTTTGGTGAC
AAAAATAATCTTCGGTGCTTGATTCCATGTGCAATAGATCAGGATCCTTATTTTAGGATGACACGAGATGTTGCACCAAGAATAGGATGTCAAAAACCTTCTCTTATTGAATCCCTGTTTTTCCCAGCGCTTCAGGGAGAGACAGGAAAAATGTCTGCAAGTGATTCCACCACAGCAATATATGTAACTGATTCTGCCAA
GGACATCAAGAACAAGGTGAATAAATATGCCTTCTCTGGAGGTGGAGATACAATAGAGAATCATAGGAAATTCGGGGCGAATCTTGAGGTGGATATATCGGTCAAGTATCTTAGCTTCTTTCTTGAGGATGATGCAGAATTGGAAAACATTAAACAAGAATATGGAGCTGGAAGAATGCTCACTGGAGAGGTTAAAAAGC
TGCTTATTGAAGTTTTGACAGACATGGTTGAACGGCATCGTAAAGCTCGAGCTGCAGTAACAGATGAGATGGTCGATGTATTTATGGCTGCAAGGCCTCTTCCTGGTATGTTTGGTTAAGGACGTTTCTCTTTCGACCATTTTGTTAGAGAATACAGAGTTTCACTTGGCATATCTAAGTTCCAATTTTTTTCCGAGTTG
TCTCTTCATTTCATGCTGTGAAATTAAAGTTGTTAGGACAAAGTTAAAGCCATTGAGTTAAATTTCCCATTGTAAGAAAATCACTTTCGTCAAAAGGTTATTTGCAAAATTAGACTGCAAGCACTTTGAATTGGTCAGAGATCAATTCAACCAGTCAAAATTGTACAGTATTCGTAATTGCCATATTGACATGGATGGGA
GAATCTCAATAAAAGTTTGACTATGTGCAGTCACTTTGCAAAATTGTACAGTATTCGTAATTGCCATATTGACATGGATGGGAGAATCTCAATAAAAGTTTGACTATGTGCAGTCACTTTGCAATCAGAACATGTTGTAGCAATTTATGCCGCCGACATAGATTAAGATTTATACTAATTTTCTCAGGAGAGTCCATTCT
CGTTGCA

Intron 2 GTCTGCAAAG ... GCTATTTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.74	81	23	22
A2	18.46	53	15	9
A3	18.34	69	20	11
A4	19.15	57	16	11
A5	17.86	23	4	6
A6	17.57	122	22	30
A7	16.67	190	43	33
A8	17.37	226	46	49
A9	17.38	252	61	45
A10	20.91	174	42	50

>TRINITY_DN39988_c0_g1_i2 from sample A7 / Example spliced isoform

AGAATATTTGTATTAGCTGCTCCGAATTGTATTTGTATTGCATATAAAAGATACGAGCGAGCAAACAGCATGTATTGACCGGAAGACCGTGATATATTGTGTCACATGTGGTGATGATAAAGGATTTCAGAAGAGTCCAACCCTCCTTTCATTCTCTCCAGTATCACTACCACTTCCATTCTCTGCAGTTTCACTACCAC
GTTTATAGGGAATTCAACCAAGAGGCGGACCTCCTGTCAAAAATAAGCGTTGAATTATCCAAAAGGGGAGGGGAAGATGGGAGGGAGCCATGAGAATGAGAATAAGAATGCAGAGGATGAAAACCAGATCGTGACTCCATGGGAAGTAGCTGCGAAGGAGGGAGGGCGTATTGATTATGACAAGCTCATTGTGCAATTCG
GCTGTCAAAAACTAGATCAATCCATTATTCAAAGGGTGGAAAAACTCACTGGCAAGCCTGCCCATGTCTTCCTCCGCCGTGGTGTTTTCTTCGCTCACAGGGAATTTGACAAGATTCTTGATGCATTTGAGAAAGGAGAAAAGTTTTATTTATATACTGGCAGAGGACCTTCATCTGAAGCTTTGCATCTGGGACATCTA
GTGCCATTCATGTTTACAAAATATTTGCAAGATGCATTCAAAGTACCACTTGTAATACAACTCACCGATGATGAGAAATGCATGTGGAAGAATCTTACTGTTGAAGAAAGTCAGAGACTTGCCCGTGAAAATGCCAAAGATATAATTGCATGTGGTTTTGATATTTCAAGGACATTCATATTTTCTGATTTTGACTATGT
TGGGGGTGCCTTCTACAAGAACATGGTTAGAATTGGCAAATGTGTCACCTTGAATCAGGTAAGAGCAATATTTGGCTTCACTGGGGAGGACCACATTGGAAAAGTCAGTTTTCCACCTGTACAAGCGGCTCCATCATTTCCATCTTCATTCCCTCATTTGTTTGGTGACAAAAATAATCTTCGGTGCTTGATTCCATGTG
CAATAGATCAGGATCCTTATTTTAGGATGACACGAGATGTTGCACCAAGAATAGGATGTCAAAAACCTTCTCTTATTGAATCCCTGTTTTTCCCAGCGCTTCAGGGAGAGACAGGAAAAATGTCTGCAAGTGATTCCACCACAGCAATATATGTAACTGATTCTGCCAAGGACATCAAGAACAAGGTGAATAAATATGCC
TTCTCTGGAGGTGGAGATACAATAGAGAATCATAGGAAATTCGGGGCGAATCTTGAGGTGGATATATCGGTCAAGTATCTTAGCTTCTTTCTTGAGGATGATGCAGAATTGGAAAACATTAAACAAGAATATGGAGCTGGAAGAATGCTCACTGGAGAGGTTAAAAAGCTGCTTATTGAAGTTTTGACAGACATGGTTGA
ACGGCATCGTAAAGCTCGAGCTGCAGTAACAGATGAGATGGTCGATGTATTTATGGCTGCAAGGCCTCTTCCTGGTATGTTTGGTTAAGGACGTTTCTCTTTCGACCATTTTGTTAGAGAATACAGAGTTTCACTTGGCATATCTAAGTTCCAATTTTTTTCCGAGTTGTCTCTTCATTTCATGCTGTGAAATTAAAGTT
GTTAGGACAAAGTTAAAGCCATTGAGTTAAATTTCCCATTGTAAGAAAATCACTTTCGTCAAAAGGTTATTTGCAAAATTAGACTGCAAGCACTTTGAATTGGTCAGAGATCAATTCAACCAGTCAAAATTGTACAGTATTCGTAATTGCCATATTGACATGGATGGGAGAATCTCAATAAAAGTTTGACTATGTGCAGT
CACTTTGCAATCAGAACATGTTGTAGCAATTTATGCCGCCGACATAGATTAAGATTTATACTAATTTTCTCAGGAGAGTCCATTCTCGTTGCAAGCTTAAATATTGGACTCGACACACTTCTTACTTTCGTTTGTTTGATTTCTGAACTAATAGATTGTAACACTTAAATTTTGTATCTGCTAGTGTACTAGATAAATCA
CTCTAATATCTAGCCTTCAATACGATATACTATTCATATAGTTAGCGAAGTCATACTTTAAAGAAAACCTCAGCGACGTCATAATTCAAAGAAAAGCCTTTCATAGGATATATCATTCAAATAGTTAGCGATATCAATCAAAGAAAACCATCGAGTGATATGGTACATGGAAAGATTTACCTTATACAATATAGAATAAG
AATGTATCTGCATGCCTAATGGTCTCATTGTTATAGCTATTGTATGTAC

>TRINITY_DN39988_c0_g1_i1 from sample A7 / Example unspliced isoform:

AGAATATTTGTATTAGCTGCTCCGAATTGTATTTGTATTGCATATAAAAGATACGAGCGAGCAAACAGCATGTATTGACCGGAAGACCGTGATATATTGTGTCACATGTGGTGATGATAAAGGATTTCAGAAGAGTCCAACCCTCCTTTCATTCTCTCCAGTATCACTACCACTTCCATTCTCTGCAGTTTCACTACCAC
GTTTATAGGGAATTCAACCAAGAGGCGGACCTCCTGTCAAAAATAAGCGTTGAATTATCCAAAAGGGGAGGGGAAGATGGGAGGGAGCCATGAGAATGAGAATAAGAATGCAGAGGATGAAAACCAGATCGTGACTCCATGGGAAGTAGCTGCGAAGGAGGGAGGGCGTATTGATTATGACAAGCTCATTGTGCAATTCG
GCTGTCAAAAACTAGATCAATCCATTATTCAAAGGGTGGAAAAACTCACTGGCAAGCCTGCCCATGTCTTCCTCCGCCGTGGTGTTTTCTTCGCTCACAGGGAATTTGACAAGATTCTTGATGCATTTGAGAAAGGAGAAAAGTTTTATTTATATACTGGCAGAGGACCTTCATCTGAAGCTTTGCATCTGGGACATCTA
GTGCCATTCATGTTTACAAAATATTTGCAAGATGCATTCAAAGTACCACTTGTAATACAACTCACCGATGATGAGAAATGCATGTGGAAGAATCTTACTGTTGAAGAAAGTCAGAGACTTGCCCGTGAAAATGCCAAAGATATAATTGCATGTGGTTTTGATATTTCAAGGACATTCATATTTTCTGATTTTGACTATGT
TGGGGGTGCCTTCTACAAGAACATGGTTAGAATTGGCAAATGTGTCACCTTGAATCAGGTAAGAGCAATATTTGGCTTCACTGGGGAGGACCACATTGGAAAAGTCAGTTTTCCACCTGTACAAGCGGCTCCATCATTTCCATCTTCATTCCCTCATTTGTTTGGTGACAAAAATAATCTTCGGTGCTTGATTCCATGTG
CAATAGATCAGGATCCTTATTTTAGGATGACACGAGATGTTGCACCAAGAATAGGATGTCAAAAACCTTCTCTTATTGAATCCCTGTTTTTCCCAGCGCTTCAGgtctgcaaaggaagctcctccttaagactacatggcctccttctcctgactcaagttctccatcataaccttgtgaaatatgtgcatcataagagg
gttcaaacacatttattttctaataattggggttgaaacatctctcctccttgtggaaacttgttgcccttgatgaagccagcttcaaagtatttcattcccacatggctcctagtggcttgtctaatcacttgagcaacttttcctgaatttcgctagtctttatatgtccctagtttacatttgtgtacgttcttgag
aataaatgtcagtaggtagtatccacaaattatgcaatggatcatttagctatttgagGGAGAGACAGGAAAAATGTCTGCAAGTGATTCCACCACAGCAATATATGTAACTGATTCTGCCAAGGACATCAAGAACAAGGTGAATAAATATGCCTTCTCTGGAGGTGGAGATACAATAGAGAATCATAGGAAATTCGGGG
CGAATCTTGAGGTGGATATATCGGTCAAGTATCTTAGCTTCTTTCTTGAGGATGATGCAGAATTGGAAAACATTAAACAAGAATATGGAGCTGGAAGAATGCTCACTGGAGAGGTTAAAAAGCTGCTTATTGAAGTTTTGACAGACATGGTTGAACGGCATCGTAAAGCTCGAGCTGCAGTAACAGATGAGATGGTCGAT
GTATTTATGGCTGCAAGGCCTCTTCCTGGTATGTTTGGTTAAGGACGTTTCTCTTTCGACCATTTTGTTAGAGAATACAGAGTTTCACTTGGCATATCTAAGTTCCAATTTTTTTCCGAGTTGTCTCTTCATTTCATGCTGTGAAATTAAAGTTGTTAGGACAAAGTTAAAGCCATTGAGTTAAATTTCCCATTGTAAGA
AAATCACTTTCGTCAAAAGGTTATTTGCAAAATTAGACTGCAAGCACTTTGAATTGGTCAGAGATCAATTCAACCAGTCAAAATTGTACAGTATTCGTAATTGCCATATTGACATGGATGGGAGAATCTCAATAAAAGTTTGACTATGTGCAGTCACTTTGCAATCAGAACATGTTGTAGCAATTTATGCCGCCGACATA
GATTAAGATTTATACTAATTTTCTCAGGAGAGTCCATTCTCGTTGCAAGCTTAAATATTGGACTCGACACACTTCTTACTTTCGTTTGTTTGATTTCTGAACTAATAGATTGTAACACTTAAATTTTGTATCTGCTAGTGTACTAGATAAATCACTCTAATATCTAGCCTTCAATACGATATACTATTCATATAGTTAGC
GAAGTCATACTTTAAAGAAAACCTCAGCGACGTCATAATTCAAAGAAAAGCCTTTCATAGGATATATCATTCAAATAGTTAGCGATATCAATCAAAGAAAACCATCGAGTGATATGGTACATGGAAAGATTTACCTTATACAATATAGAATAAGAATGTATCTGCATGCCTAATGGTCTCATTGTTATAGCTATTGTATG
TAC