sp|Q851Q6|OBGC2_ORYSJ ----> Probable GTP-binding protein OBGC2

Intron 1 GTATGGCTAT ... ATTTATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.68	56	1	6
A2	19.23	42	0	10
A3	9.09	30	0	3
A4	0	39	0	0
A5	4	24	0	1
A6	2.27	129	0	3
A7	6.6	184	0	13
A8	3.64	159	0	6
A9	7.5	185	0	15
A10	5.38	211	0	12

>TRINITY_DN119925_c3_g6_i6 from sample A1 / Example spliced isoform

TATGCATTGATGGCTAGTCGACGGTGCGTGGATAGACGTCTCTTCCTTGCCTTGAGGTTGTTGTGGGAGGTTGCTGTTGTACCATGGACTGCTTCGTTGCCATGCCTCTGGCTCTTCCTCGTTCCAAATTCAAAATTCTACCTGCCTGGACAGGACTCACTGCACATTCCCTCCAACTCTCCAAATCAAGTGCTACAGTT
GCATTTCATGTAAAAGGCAAAGATGCAGGGACATGCATTCAAGGTCATGTGATACAGTGCATATTGACCAAGGTTAAAGATTCATCTACTCCTATTTTACATAACTTGGAAAGAGAGCCTCATAAATATTTTGATCATGTTGTCATTACTGTGCGCTCTGGAGACGGGGGGCATGGTGCTGTTCTTAGCATGCCCATGCC
TAAAATGCAAGTTAAAAGTTCATTGAGAAAGAAAAAAGAGAAAGAAAAAAGAAAAGGCTCATTTAGACGGGATCCTGATGGAACCTTGATTTTACCTATGGGTGGACATGGAGGAGATGTTGTACTCTATGCAGACGAGAGCAAAGATTCCTTACTGGAGCTTCACCAGATCAGAAGATACAATGCCAAGCGTGGAGGGA
ATGTTGATGCTATGGGTGTTTTGAGTCCCAGGTTGCATGATGGATTTATACCACCAACAGTGCGAATTCCAGTCCCTGTAGGTACTGTAGTGAAGCGTAAGCGAGGGGGAAAGCTTCTTGCAGATCTTGCTCGTCCTGGTGATCAGATACTTGTTGCAAAGGGTGGGCGAGGAGGGATTAGTTTGGTGGAAACATCTCAA
AGTAATAGGAGTCAGTTGAGAGCTGTAAATGTGAACGTCATGAGAGATAAAAGTGACAAGGTTATGGTATTTGGACAACCAGGGGAAGAAGTAACATTGGAGCTAATATTGCGAATTGTGGCAGATGTTGGTCTTGTGGGACTTCCAAATGCAGGAAAGTCTTCTCTTTTGGCATCTGTGACACTTGCAAAGCCAGAGAT
TGCAAATTATCCTTTCACAACATTGATGCCAAATCTTGGACGCCTTGATGGGGATCCTGATTTGGGAACTTGGGGGTTTTCATCTCGTGCAACATTGGCAGACTTACCTGGTCTCATAAGAGGGGCCCATTTGGGGAAGGGTCTTGGCAGAACATTCTTAAGACACCTCCGCAGAACTCGACTTTTGGTTCATGTTGTTG
ATGCGTCTGCAGATGATCCTGTGCAAGATTATAGAACTCTTAGAGAAGAATTATGTATGTACAACCCAGATTATCTTAAAAGGCCCCACATGGTGGTTTTAAACAAGCTTGATCTCCCAGAGGCAAAGGAGAGGTTTGATTCTATGTCCAAACAGATAGAAAGAATTGGTTCCCTTGAAGATGTACATGGAGGTGCGCAC
ATGTTGGCAAAGGAGAGGTTTGATTCTATGTCCAAACAGATAGAAAGAATTGGTTCCCTTGAAGATGTACATGGAGGTGCGCACATGTTGGTAGAAGGAAACGGTTGTGGGGACATCGAAGCTTGCATAAATTCTGAGGGAGACAATGAAGCTACTTCTCTAAAGCAAGAAAAGAAGATGGAAGACTATCCTCGTCCTGT
AGCGGTTGTTGGCATAAGTGCTTTGAAAGGAACTGGCATTGTTCATATGTTGAAGCAAATTCGATTGGCACTAAAAGCATGTGAACCAAACAATAATAAAGGCAATGAAACTGAGATGACAAGCCCCATATTGTACCAATAATCCAAATCGTAGATCTAATCTATTAGGTCATGCATTGAAACTTTGAAAGTATTTCTCT
TGGTAGTTTCGTGGCAATATGCAAGTAAATATTAATTTTTGTAGAGAAATTATTGTCTTTCTATGATTTGTTAAGCAACACAGATTTTTATATCGAGCACTTTCTTGTTAGTTCTAGCGATTTGTTAAAGAGAGGGTAATACAAATCCTCACTTCTATGATTAGTCAAGGCTTAACTGTTGTCAATGTCATGATCTGTTT
AATGTTGTGGTCACTTCATATAGAGGTTTATCTTATTTAGATTCTTGGGATCTATTCAAGAACTGAAAAGTGTGACAATAGTGTTCATGTTCCTATATGTTATGTATTATGTAAGCAACTCATACGTGC

>TRINITY_DN119925_c3_g6_i3 from sample A1 / Example unspliced isoform:

AAGAGGGGCCCATTTGGGGAAGgtatggctatttttatcatactacatagttactttgggctttcagatatttacatttctgttatacttgatttaccgtagcttttgcatttttcatttggaaagtctaaaattgtaaatttatgtagGGTCTTGGCAGAACATTCTTAAGACACCTCCGCAGAACTCGACTTTTGGTT
CATGTTGTTGATGCGTCTGCAGATGATCCTGTGCAAGATTATAGAACTCTTAGAGAAGTATTGCCTCTCACTTACAACTTTTTGTTCCTTAAAATAGTTTGGAGAATTGATTGCTCATAATCCCA

Intron 2 GTTAAAGATT ... CTCTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.56	31	53	65
A2	55.56	42	48	57
A3	61.45	53	84	85
A4	69.07	30	70	64
A5	62.83	21	32	39
A6	66.45	52	92	114
A7	58.84	114	148	178
A8	65.1	63	79	156
A9	59.61	103	157	147
A10	61.8	85	88	187

>TRINITY_DN109606_c0_g1_i5 from sample A2 / Example spliced isoform

GTGCGTGGATAGACGTCTCTTCCTTGCCTTGAGGTTGTTGTGGGAGGTTGCTGTTGTACCATGGACTGCTTCGTTGCCATGCCTCTGGCTCTTCCTCGTTCCAAATTCAAAATTCTACCTGCCTGGACAGGACTCACTGCACATTCCCTCCAACTCTCCAAATCAAGTGCTACAGTTGCATTTCATGTAAAAGGCAGGCA
AAGATGCAGGGACATGCATTCAAGGTCATGTGATACAGTGCATATTGACCAAGACGAGAGCAAAGATTCCTTACTGGAGCTTCACCAGATCAGAAGATACAATGCCAAGCGTGGAGGGAATGTTGATGCTATGGGTGTTTTGAGTCCCAGGTTGCATGATGGATTTATACCACCAACAGTGCGAATTCCAGTCCCTGTAG
GTACTGTAGTGAAGCGTAAGCGAGGGGGAAAGCTTCTTGCAGATCTTGCTCGTCCTGGTGATCAGATACTTGTTGCAAAGGGTGGGCGAGGAGGGATTAGTTTGGTGGAAACATCTCAAAGTAATAGGAGTCAGTTGAGAGCTGTAAATGTGAACGTCATGAGAGATAAAAGTGACAAGGTTATGGTATTTGGACAACCA
GGGGAAGAAGTAACATTGGAGCTAATATTGCGAATTGTGGCAGATGTTGGTCTTGTGGGACTTCCAAATGCAGGAAAGTCTTCTCTTTTGGCATCTGTGACACTTGCAAAGCCAGAGATTGCAAATTATCCTTTCACAACATTGATGCCAAATCTTGGACGCCTTGATGGGGATCCTGATTTGGGAACTTGGGGGTTTTC
ATCTCGTGCAACATTGGCAGACTTACCTGGTCTCATAAGAGGGGCCCATTTGGGGAAGGGTCTTGGCAGAACATTCTTAAGACACCTCCGCAGAACTCGACTTTTGGTTCATGTTGTTGATGCGTCTGCAGATGATCCTGTGCAAGATTATAGAACTCTTAGAGAAGAATTATGTATGTACAACCCAGATTATCTTAAAA
GGCCCCACATGGTGGTTTTAAACAAGCTTGATCTCCCAGAGGCAAAGGAGAGGTTTGATTCTATGTCCAAACAGATAGAAAGAATTGGTTCCCTTGAAGATGTACATGGAGGTGCGCACATGTTGGTAGAAGGAAACGGTTGTGGGGACATCGAAGCTTGCATAAATTCTGAGGGAGACAATGAAGCTACTTCTCTAAAG
CAAGAAAAGAAGATGGAAGACTATCCTCGTCCTGTAGCGGTTGTTGGCATAAGTGCTTTGAAAGGAACTGGCATTGTTCATATGTTGAAGCAAATTCGATTGGCACTAAAAGCATGTGAACCAAACAATAATAAAGGCAATGAAACTGAGATGACAAGCCCCATATTGTACCAATAATCCAAATCGTAGATCTAATCTAT
TTGGTCATGCATTGCAACTTTGAAAGTATTTCTCTTGGTAGTTTCGTGGCAATATGCAAGTAAATATTAATTTTTGTAGAGAGATTATAGTCTTTCTATGATTTGTTAAGCAACACAGATTTTATCGAGCACTTTCTTGTTAGTTCTACTGATTTGTTAAAGAGAGGGTAATACAAATCCTCACTTCTATGATTAGTCAA
GGCTTAACTGTTGTCAATGTCATGATCTGTTTAATGTTGTGGTCACTTCATATAGAGGTTTATCTTATTTAGATTCTTGGGATCTATTCAAGAACTGAAAAGTGTGACAATAGTGTTCATGTTCCTATATGTTATGTATTATGTAAGCAACTCATACGTGCAAAAGTGAAGAAGC

>TRINITY_DN109606_c0_g1_i8 from sample A2 / Example unspliced isoform:

GTGCGTGGATAGACGTCTCTTCCTTGCCTTGAGGTTGTTGTGGGAGGTTGCTGTTGTACCATGGACTGCTTCGTTGCCATGCCTCTGGCTCTTCCTCGTTCCAAATTCAAAATTCTACCTGCCTGGACAGGACTCACTGCACATTCCCTCCAACTCTCCAAATCAAGTGCTACAGTTGCATTTCATGTAAAAGGCAAAGA
TGCAGGGACATGCATTCAAGGTCATGTGATACAGTGCATATTGACCAAGgttaaagattcatctactcctattttacataacttggaaagagagcctcataaatattttgatcatgttgtcattactgtgcgctctggagacggggggcatggtgctgttcttagcatgcccatgcctaaaatgcaagttaaaagttcat
tgagaaagaaaaaagagaaagaaaaaagaaaaggctcatttagacgggatcctgatggaaccttgattttacctatgggtggacatggaggagatgttgtactctatgcagACGAGAGCAAAGATTCCTTACTGGAGCTTCACCAGATCAGAAGATACAATGCCAAGCGTGGAGGGAATGTTGATGCTATGGGTGTTTTG
AGTCCCAGGTTGCATGATGGATTTATACCACCAACAGTGCGAATTCCAGTCCCTGTAGGTACTGTAGTGAAGCGTAAGCGAGGGGGAAAGCTTCTTGCAGATCTTGCTCGTCCTGGTGATCAGATACTTGTTGCAAAGGGTGGGCGAGGAGGGATTAGTTTGGTGGAAACATCTCAAAGTAATAGGAGTCAGTTGAGAGC
TGTAAATGTGAACGTCATGAGAGATAAAAGTGACAAGGTTATGGTATTTGGACAACCAGGGGAAGAAGTAACATTGGAGCTAATATTGCGAATTGTGGCAGATGTTGGTCTTGTGGGACTTCCAAATGCAGGAAAGTCTTCTCTTTTGGCATCTGTGACACTTGCAAAGCCAGAGATTGCAAATTATCCTTTCACAACAT
TGATGCCAAATCTTGGACGCCTTGATGGGGATCCTGATTTGGGAACTTGGGGGTTTTCATCTCGTGCAACATTGGCAGACTTACCTGGTCTCATAAGAGGGGCCCATTTGGGGAAGGGTCTTGGCAGAACATTCTTAAGACACCTCCGCAGAACTCGACTTTTGGTTCATGTTGTTGATGCGTCTGCAGATGATCCTGTG
CAAGATTATAGAACTCTTAGAGAAGAATTATGTATGTACAACCCAGATTATCTTAAAAGGCCCCACATGGTGGTTTTAAACAAGCTTGATCTCCCAGAGGCAAAGGAGAGGTTTGATTCTATGTCCAAACAGATAGAAAGAATTGGTTCCCTTGAAGATGTACATGGAGGTGCGCACATGTTGGTAGAAGGAAACGGTTG
TGGGGACATCGAAGCTTGCATAAATTCTGAGGGAGACAATGAAGCTACTTCTCTAAAGCAAGAAAAGAAGATGGAAGACTATCCTCGTCCTGTAGCGGTTGTTGGCATAAGTGCTTTGAAAGGAACTGGCATTGTTCATATGTTGAAGCAAATTCGATTGGCACTAAAAGCATGTGAACCAAACAATAATAAAGGCAATG
AAACTGAGATGACAAGCCCCATATTGTACCAATAATCCAAATCGTAGATCTAATCTATTTGGTCATGCATTGCAACTTTGAAAGTATTTCTCTTGGTAGTTTCGTGGCAATATGCAAGTAAATATTAATTTTTGTAGAGAGATTATAGTCTTTCTATGATTTGTTAAGCAACACAGATTTTATCGAGCACTTTCTTGTTA
GTTCTACTGATTTGTTAAAGAGAGGGTAATACAAATCCTCACTTCTATGATTAGTCAAGGCTTAACTGTTGTCAATGTCATGATCTGTTTAATGTTGTGGTCACTTCATATAGAGGTTTATCTTATTTAGATTCTTGGGATCTATTCAAGAACTGAAAAGTGTGACAATAGTGTTCATGTTCCTATATGTTATGTATTAT
GTAAGCAACTCATACGTGCAAAAGTGAAGAAGC