sp|F4KDH9|FIPS5_ARATH ----> FIP1[V]-like protein


Intron 1 GTAAGTAATA ... CACTCTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A134.1554283
A242.1937278
A347.37504515.6
A435.94412313
A5727183
A678.954153
A752.6318206
A8608123
A958.0613185
A1070.2114330



>TRINITY_DN113159_c0_g1_i6 from sample A3 / Example spliced isoform
GGAACTTTTAAAATCCCACTCCAACGATATTTCCAAATACTACCGAGCCCACTTTGCCGAAAATGAGGTTGTTACTCCTATGTATTTCCAAATACTACTACATTCGAATAATAATTAGAAGTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCA
GATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATG
CAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCTCTTCCAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTT
CGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCC
TGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATG
AACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGG
GAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAA
CGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATGGATGGGATAGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAG
GAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCA
GGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGA
TTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCTTCCTCCTAACAAGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAA
CGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACA
CAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATC
AGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTAC
CTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCA
AATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAG
CAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAG
GTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAAC
CACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGG
TTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAG
ATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAA
AAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGG
TCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGA
CATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGG
GTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGC
TTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGGAAAGCTTTCCTATATAATACCAGAACTATATTGATGTCTATTCATCAAATCGTATGATATGGAGGAAATCCCGCCTTCGGAGAGAAGCACTGAAGACCAAGA
ATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTC
AGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAG
TGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACCCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGA
TGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGAT
TGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTT
CAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATG
CAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTC
TCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCAT
TCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGGCTAC


>TRINITY_DN113159_c0_g1_i5 from sample A3 / Example unspliced isoform:
GGAACTTTTAAAATCCCACTCCAACGATATTTCCAAATACTACCGAGCCCACTTTGCCGAAAATGAGGTTGTTACTCCTATGTATTTCCAAATACTACTACATTCGAATAATAATTAGAAGTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCA
GATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATG
CAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAA
ATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCT
CAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAA
AGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCC
GAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTT
GCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATGGATGGGATAGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGA
CGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTG
GCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGT
GGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCTTCCTCCTAACAAgtaagtaataggtgatttttatatattgtaccataataatatcatgcattttgacatcaaactttagagggagtggttgtagag
gttgggattgtaaaatgtggatcagtaatataggacaacaaaatgttcctctgggaagaagccctttggctctatgtaaccatgtaagtgcttcttagcaggtcttgtaactgttaaaaatgttatttgaatgtattagtctctctgttgaaggtgatggctaattttggtatctcccatcaggatctcttataaattat
gctgttaatacatacactagcttcaaaatatatgtgtagtaggaggaagcttcgctttcatttaaaaaggcagtctcaactatcaaaaaatgtagtttgttttctagagcaccggttgcaaatcttgccacaggcccacaactgtggataataccatgttcggtgacatcttgatcaagcagctgaagttttgcacttga
cggcaaaagcaagttgatgaactgatcccgcttgacgtttgattttgccaaggcctttttctcttgattagattggattctacattgatgtcagtgtaatattttatgaattttactatctcatattgtaaaagatttgagcttcaggcatcctagcaaggtcccttgcttcatcctggcaccatcccttttctgaacaa
tgctatattggtatttttggggggtcattgtcaacaaatggaaatagcctggtcctggtcagtaactttgtcaagatgccggtttgtataccagagagctgcttccattctttgccttaatcagggctctgcagatggttatttcttgttggtaacttggatataaatgggtgctacacatgcatttgcatttaagcgac
tctcatcatcatccagcgacagtagcacatcatttgtgcacatgctgacctctgggagaataatatcagatattttagtgtgtggttgctgaaggatatttttcttctgtgatactgatgaggtgtgttgccactgcatttggcaaatgcaggcatgtaaatggagttgctttgaaatgtcaatttgatttgtttaaaga
ccatttttgggaagtttggacttgtttgaagtgacttgttcagtttatgatctttaatttacaattgctgcttgcagagatgctgctgcgttatcaaaatttcttactgtacattgtttagtgaccttttctatagagtttgtgatgcttgcaaaatcacatatttatattaaatttcaattgatacacataatgcaagt
ttcaaacttcaaaggtttgatatttctatgggttattccatgccaacgttgccaacttaccaagatagcaggttgaaggtgttgattatccatttttccctcgatgctcactctgcagGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAAC
TTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGTATGATCTGTTCTGACAGATATATAAATACAAATTGCTTCCCTTATATTGCTTTTTCTGTTTTAGGATGTTAGTGAACTGCAAAGCTTGGAATTCAAGGGCTCATTTTCTCAGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCG
TGTCTATGAAAGTGGACGTTCTGAGCAGGTAGTCAATCTAAAGACAATAAATATATGTGTGCTGTATTTTTAAGTGTTATCGTTTCTCA



Intron 2 GTATGCTATT ... CAACTGGTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A19.097071
A229.5531130
A312.774160
A420.933490
A533.331683
A657.14345
A729.032292
A84511910
A922.221441
A107.893530



>TRINITY_DN45506_c0_g1_i2 from sample A8 / Example spliced isoform
GTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATGCAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAG
GGTTCCAATCTGGACAAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCG
CAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATG
CCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATAC
AGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTC
CGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATGGATGGGAT
AGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGTCGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGT
ATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCTTGGGGGAATTTCAAGTGCTT
CTAGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTT
GAATTTTCTCTTCCTCCTAACAAGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAATTGGCAGGAGTATTGCAGAAAAATGGAACAACTTACGTTGGAGGGAACCATGCAAAGCAAAATTTGTGTCTATGA
AAGTGGACGTTCTAAACAGGAGTATGATCTGGACCTACCAATTGAGCTGGAAACTGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAGCTGTCTGATCCAGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTAGCTATTTAGGTTGAAGGTGGTGTTGGTGAA
AGGCGTCCTTCAGTAGATTTTAGATGGCCACGCTTTCATGATTCTAATGTTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCATTTCCCCTTTGAAATCACCTGAGAGTGCAAATGATGTTAATGAGAGAGAGGATTCAGAAAGGGGAGAGACTAAAATGGGATGCCAAAAACAATTTAACACAGAGTC
TTCCTCACAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACACCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCTTCTGATTCATTATCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATG
CTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCC
ACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTA
TTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACT
ATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGCAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTG
GGGAAAAATGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGAT
TGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAA
GGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGG
ATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAG
TCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAG
TGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTT
CACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCAT
GAAAAAAAGGTAATCTCGATACCTTTTTGTCAGAAAATTGATGCCTCCTCGAACACTATATAGGTTGTTTCTTATCCTGTGAAATATAGAATGACGTTTTTAGCTGTGTCAATGTAATATTAGTAAACACTGTTTCAGGAGTCTGGGAAGTTTGGACAGATGATACTAAGACGTTCAGTGTTGAGATTAATCTCAATTAT
TTTGTAACAGGCTATGGAAAGCTTAATTCTGTTTGATACAGCATTCCATGTTTACAAAGAAATAAATGCCTGGAAAGAGTTATATTTCTTCCTATCATTTTACATTATAATCTGAGCTATAAAATGCTGTT


>TRINITY_DN45506_c0_g1_i5 from sample A8 / Example unspliced isoform:
ATTGGCAGGAGTATTGCAGAAAAATGGAACAACTTACGTTGGAGGGAACCATGCAAAGCAAAATTTGTGTCTATGAAAGTGGACGTTCTAAACAGGAGTATGATCTGGACCTACCAATTGAGCTGGAAACTGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAGCTGTCTGATCCAGGATCTGCAGCT
TCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTgtatgctattgctacatgctcctttcacagtgctggtctttaattagaatatgcttgtgatgttatatagattgtgagcatagattcaatacatttatgatctattaaatgttgcaacatgtttttgtgcaaccaactggtagAGCTATTTAGGT
TGAAGGTGGTGTTGGTGAAAGGCGTCCTTCAGTAGATTTTAGATGGCCACGCTTTCATGATTCTAATGTTGTAATAGAGGTAAATGAATCTTTCTCTTGTGATGTCATCTCA



Intron 3 GTATGATCTG ... ATTTTCTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.05731546.2
A217.46521117.3
A316.1175.914.637.2
A423.61551716.7
A521.882576.4
A60100
A713.1319.936.2
A88.331113.1
A927.781354.7
A105.881617



>TRINITY_DN126812_c1_g2_i1 from sample A1 / Example spliced isoform
GTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCAGATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGC
CTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATGCAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCT
CTTCCAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAAT
CCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCC
CCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGA
TGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGC
ATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTGCAAGAAGAGAATGGATGGGATAGTGACAGCGA
AGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTG
CCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGTTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCC
CAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCT
TCCTCCTAACAAGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTT
CTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAA
AGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTC
TTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATG
CTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCC
ACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTA
TTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACT
ATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTG
GGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAA
GCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAG
AAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAA
AAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCG
GGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAG
CTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGAT
CATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGGAAAGCTTTCCTATATAATACCA
GAACTATATTGATGTCTATTCATCAAATCGTATGATATGGAGGAAATCCCGCCTTCGGAGAGAAGCACTGAAGACCAAGAATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAA
ACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTCAGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTG
TTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAGTGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAG
CGTTTTAAACAACCGATTCCAAATGAAAAGGAAACTCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGATGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATT
TAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGATTGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGG
ACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTTCAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGA
TCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATGCAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAG
GTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTT
ACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCATTCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGG


>TRINITY_DN126812_c1_g2_i2 from sample A1 / Example unspliced isoform:
GTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCAGATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGC
CTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATGCAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCT
CTTCCAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAAT
CCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCC
CCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGA
TGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGC
ATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTGCAAGAAGAGAATGGATGGGATAGTGACAGCGA
AGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTG
CCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGTTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCC
CAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCT
TCCTCCTAACAAGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGgtatgatctgttctgacagatatataaatacaaattgcttcccttatattgctttttctgt
tttaggatgttagtgaactgcaaagcttggaattcaagggctcattttctcagGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCAT
AAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGA
CGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAA
CACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAA
GATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACC
AAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATA
GCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCAT
AGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCA
ACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCC
TTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCAT
GAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCG
GGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATG
GTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGAT
GATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGG
CTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGGAAAGCTTTCCTATATAATACCAGAACTATATTGATGTCTATTCATCAAATCGTATGATATGGAGGAAATCCCGCCTTCGGAGAGAAGCACTGAAGACCAAGAATCGGC
AAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTCAGAAAG
AAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAGTGCAAG
GAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACTCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGATGAAGT
TAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGATTGCCAT
GGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTTCAAAGA
GCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATGCAGACA
GACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTG
AATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCATTCTTTC
TCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGG



Intron 4 GTACGATTCA ... GCTCTTCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A112.061132011
A215.92661015
A315.53872210
A418.6448814
A518.7539810
A65.26910
A733.33101
A80500
A933.33220
A1018.521123



>TRINITY_DN113159_c0_g1_i8 from sample A3 / Example spliced isoform
GGAACTTTTAAAATCCCACTCCAACGATATTTCCAAATACTACCGAGCCCACTTTGCCGAAAATGAGGTTGTTACTCCTATGTATTTCCAAATACTACTACATTCGAATAATAATTAGAAGTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCA
GATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATG
CAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAA
ATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCT
CAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAA
AGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCC
GAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTT
GCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATGGATGGGATAGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGA
CGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTG
GCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGT
GGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCTTCCTCCTAACAAGTAAGTAATAGGTGATTTTTATATATTGTACCATAATAATATCATGCATTTTGACATCAAACTTTAGAGGGAGTGGTTGTAGAG
GTTGGGATTGTAAAATGTGGATCAGTAATATAGGACAACAAAATGTTCCTCTGGGAAGAAGCCCTTTGGCTCTATGTAACCATGTAAGTGCTTCTTAGCAGGTCTTGTAACTGTTAAAAATGTTATTTGAATGTATTAGTCTCTCTGTTGAAGGTGATGGCTAATTTTGGTATCTCCCATCAGGATCTCTTATAAATTAT
GCTGTTAATACATACACTAGCTTCAAAATATATGTGTAGTAGGAGGAAGCTTCGCTTTCATTTAAAAAGGCAGTCTCAACTATCAAAAAATGTAGTTTGTTTTCTAGAGCACCGGTTGCAAATCTTGCCACAGGCCCACAACTGTGGATAATACCATGTTCGGTGACATCTTGATCAAGCAGCTGAAGTTTTGCACTTGA
CGGCAAAAGCAAGTTGATGAACTGATCCCGCTTGACGTTTGATTTTGCCAAGGCCTTTTTCTCTTGATTAGATTGGATTCTACATTGATGTCAGTGTAATATTTTATGAATTTTACTATCTCATATTGTAAAAGATTTGAGCTTCAGGCATCCTAGCAAGGTCCCTTGCTTCATCCTGGCACCATCCCTTTTCTGAACAA
TGCTATATTGGTATTTTTGGGGGGTCATTGTCAACAAATGGAAATAGCCTGGTCCTGGTCAGTAACTTTGTCAAGATGCCGGTTTGTATACCAGAGAGCTGCTTCCATTCTTTGCCTTAATCAGGGCTCTGCAGATGGTTATTTCTTGTTGGTAACTTGGATATAAATGGGTGCTACACATGCATTTGCATTTAAGCGAC
TCTCATCATCATCCAGCGACAGTAGCACATCATTTGTGCACATGCTGACCTCTGGGAGAATAATATCAGATATTTTAGTGTGTGGTTGCTGAAGGATATTTTTCTTCTGTGATACTGATGAGGTGTGTTGCCACTGCATTTGGCAAATGCAGGCATGTAAATGGAGTTGCTTTGAAATGTCAATTTGATTTGTTTAAAGA
CCATTTTTGGGAAGTTTGGACTTGTTTGAAGTGACTTGTTCAGTTTATGATCTTTAATTTACAATTGCTGCTTGCAGAGATGCTGCTGCGTTATCAAAATTTCTTACTGTACATTGTTTAGTGACCTTTTCTATAGAGTTTGTGATGCTTGCAAAATCACATATTTATATTAAATTTCAATTGATACACATAATGCAAGT
TTCAAACTTCAAAGGTTTGATATTTCTATGGGTTATTCCATGCCAACGTTGCCAACTTACCAAGATAGCAGGTTGAAGGTGTTGATTATCCATTTTTCCCTCGATGCTCACTCTGCAGGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAAC
TTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTC
TGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCA
TTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACAGCAT
CATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAG
AGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAG
AAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGAC
TATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGA
ACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGG
ACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAA
GATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGA
TGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAA
GAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGA
GATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATC
AAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTG
ATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGCCTTCGGAGAGAAGCACTGAAGACCAAGAATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAG
GAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTCAGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGG
AAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAGTGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTG
AGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACCCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGATGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAAT
GCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGATTGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGT
TATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTTCAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTC
CCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATGCAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGT
ATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATT
AATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCATTCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGGCTAC


>TRINITY_DN113159_c0_g1_i2 from sample A3 / Example unspliced isoform:
GGAACTTTTAAAATCCCACTCCAACGATATTTCCAAATACTACCGAGCCCACTTTGCCGAAAATGAGGTTGTTACTCCTATGTATTTCCAAATACTACTACATTCGAATAATAATTAGAAGTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCA
GATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATG
CAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGgtacgattcatctcgtcgtcggcttacaattttttttatatttttcgtattttctttaccatccaggttttgtctgattcatctcgatt
gtctggctagggtttggctcttccagAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGT
GGAGAGCGCAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCC
CTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTT
GAGAATACAGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGA
CGATATTCCGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATG
GATGGGATAGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAA
GGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTC
AAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAA
ATGGCTTTGAATTTTCTCTTCCTCCTAACAAGTAAGTAATAGGTGATTTTTATATATTGTACCATAATAATATCATGCATTTTGACATCAAACTTTAGAGGGAGTGGTTGTAGAGGTTGGGATTGTAAAATGTGGATCAGTAATATAGGACAACAAAATGTTCCTCTGGGAAGAAGCCCTTTGGCTCTATGTAACCATGT
AAGTGCTTCTTAGCAGGTCTTGTAACTGTTAAAAATGTTATTTGAATGTATTAGTCTCTCTGTTGAAGGTGATGGCTAATTTTGGTATCTCCCATCAGGATCTCTTATAAATTATGCTGTTAATACATACACTAGCTTCAAAATATATGTGTAGTAGGAGGAAGCTTCGCTTTCATTTAAAAAGGCAGTCTCAACTATCA
AAAAATGTAGTTTGTTTTCTAGAGCACCGGTTGCAAATCTTGCCACAGGCCCACAACTGTGGATAATACCATGTTCGGTGACATCTTGATCAAGCAGCTGAAGTTTTGCACTTGACGGCAAAAGCAAGTTGATGAACTGATCCCGCTTGACGTTTGATTTTGCCAAGGCCTTTTTCTCTTGATTAGATTGGATTCTACAT
TGATGTCAGTGTAATATTTTATGAATTTTACTATCTCATATTGTAAAAGATTTGAGCTTCAGGCATCCTAGCAAGGTCCCTTGCTTCATCCTGGCACCATCCCTTTTCTGAACAATGCTATATTGGTATTTTTGGGGGGTCATTGTCAACAAATGGAAATAGCCTGGTCCTGGTCAGTAACTTTGTCAAGATGCCGGTTT
GTATACCAGAGAGCTGCTTCCATTCTTTGCCTTAATCAGGGCTCTGCAGATGGTTATTTCTTGTTGGTAACTTGGATATAAATGGGTGCTACACATGCATTTGCATTTAAGCGACTCTCATCATCATCCAGCGACAGTAGCACATCATTTGTGCACATGCTGACCTCTGGGAGAATAATATCAGATATTTTAGTGTGTGG
TTGCTGAAGGATATTTTTCTTCTGTGATACTGATGAGGTGTGTTGCCACTGCATTTGGCAAATGCAGGCATGTAAATGGAGTTGCTTTGAAATGTCAATTTGATTTGTTTAAAGACCATTTTTGGGAAGTTTGGACTTGTTTGAAGTGACTTGTTCAGTTTATGATCTTTAATTTACAATTGCTGCTTGCAGAGATGCTG
CTGCGTTATCAAAATTTCTTACTGTACATTGTTTAGTGACCTTTTCTATAGAGTTTGTGATGCTTGCAAAATCACATATTTATATTAAATTTCAATTGATACACATAATGCAAGTTTCAAACTTCAAAGGTTTGATATTTCTATGGGTTATTCCATGCCAACGTTGCCAACTTACCAAGATAGCAGGTTGAAGGTGTTGA
TTATCCATTTTTCCCTCGATGCTCACTCTGCAGGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTC
GTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAG
GTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCA
AAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCA
GGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATG
GTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTC
ACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCC
AGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGA
GAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCG
GCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATT
ACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGAT
AGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACG
AACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGA
GAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGAT
CGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGCC
TTCGGAGAGAAGCACTGAAGACCAAGAATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTC
GCTCCAAATTGGAACGTTGGGCCAGTCAGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAG
CAAGATGACCATACTGCAAATCAGGAGTGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACCCAAAAGAAGGCTGAGAA
TGAAACTCTTTTAGAAAATGAGACAGATGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGG
TTCATATTAGGCAAACAATTTATTGATTGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCT
TGATGTTAAGAATATAGTGCATGTCTTCAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAAC
AATCTGTATGGGAGCAGAGGCACCATGCAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACA
TTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATC
TTTCCCATTAATAACATATAAATTCATTCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGGCTAC



Intron 5 GTAGTCAATC ... TGTCAATTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A110.51111.3013
A211.396308
A312.22100.8014
A423.261.5218
A517.2334.307
A647.116.806
A718.8321.815
A827.0424.509
A923.5619.506
A1014.752905



>TRINITY_DN126812_c1_g2_i1 from sample A1 / Example spliced isoform
GTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCAGATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGC
CTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATGCAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCT
CTTCCAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAAT
CCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCC
CCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGA
TGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGC
ATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTGCAAGAAGAGAATGGATGGGATAGTGACAGCGA
AGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTG
CCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGTTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCC
CAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCT
TCCTCCTAACAAGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTT
CTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAA
AGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTC
TTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATG
CTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCC
ACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTA
TTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACT
ATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTG
GGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAA
GCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAG
AAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAA
AAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCG
GGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAG
CTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGAT
CATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGGAAAGCTTTCCTATATAATACCA
GAACTATATTGATGTCTATTCATCAAATCGTATGATATGGAGGAAATCCCGCCTTCGGAGAGAAGCACTGAAGACCAAGAATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAA
ACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTCAGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTG
TTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAGTGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAG
CGTTTTAAACAACCGATTCCAAATGAAAAGGAAACTCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGATGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATT
TAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGATTGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGG
ACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTTCAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGA
TCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATGCAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAG
GTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTT
ACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCATTCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGG


>TRINITY_DN126812_c1_g2_i6 from sample A1 / Example unspliced isoform:
TGAAAGTGGACGTTCTGAGCAGgtagtcaatctaaagacaataaatatatgtgtgctgtatttttaagtgttatcgtttctcagtgtttgtactttgtatactcattttttgggctacctgtcaattagGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTAC
ACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCAT
CAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTA
CCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGGTGAAATTTGATACAGGACAGTCTCTTTAAGTGTTTCATAATGTTATTCAACTAGTCTTTACATGTATGCAC
TTTCAGTGAAGTCCTTGCCACAAAGTTACATTTAATCTTGCCAGGGACCTAACTGCTCCATACTTTGCTGCAAAGAAAGATTCATATTTATTCAGTTGTTTACATTTTAATAATTTATTCTTTTGTTGTAACATTGAATTTGGGGTCTGGAGATAAATTTATATTTGCTTTTGAGGCCAACTTTATGAAACTGCTTAGTC
AAGAGTGACAATCAAGTTTATAAAAGATGTATCATATTTGATAAATCTATGTTTGCTTGAAATTTGTCCACCTCCTTTGAGCACTGCTACTATGGGTACAACTAAATAACACTGCATAGAGTAGAAAATCTTATAAGAACTTAGGACTGGAATATCAGAAGCTAAGTCACAGGGTTCGGCGAATCTCAACATTTAGATTC
GAAATTCGTTGAATTCCAAAACAAAAAAGAAAATCATTGAAGTTCGGCCGCCAACTTTGTTCACTTCACCCATGGCAGGCCCGATGGTGGATGTGGTTTGTTGATTCTTGGCTGTGATTCGGGACATGGTAACTCAGTCCATGAACTCATAACCCAAGGTCCCCGTGTGAAATGTTTTACTGCACGATTCCTGGTGCGAT
TTACTATTCGATTTACAATTGGTAGTGACCGCCTGATCGGACATCGGAGATGGCAACTTTGGTGCAATTTATTATTCGATTTACTATTGGTAGCGACTGCCTGACGGGACATGGGAGACGGCGATTCAATCGACGAACACATAACCTAGGGTGCTTGCGGGAGAAGTAAATAAAATGGTACC



Intron 6 GTAAATGAAG ... ATGTGATCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A116.67601311
A216.883267
A314.065599
A419.427103
A522.581243
A611.11410
A716.281852
A8201443
A925951
A1014.892052



>TRINITY_DN114846_c4_g1_i2 from sample A2 / Example spliced isoform
GCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCAGATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCC
TCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATGCAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCTCTTCCAGAGTTGTATT
TTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAATCCGATGGAAGACGATG
ACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTA
GCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGATGGGGGAGCGCGAGGA
TGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGCATGGTGATGAATGGCG
TGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTGCAAGAAGAGAATGGATGGGATAGTGACAGCGAAGATGATTTGCAAATT
GTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACC
AGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGC
ATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCTTCCTCCTAACAAGACA
GTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGA
TCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGG
ATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGG
CATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGATGGTCACA
GTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGT
GCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAG
GTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTT
GTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGAAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATA
TGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAG
GATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAG
AGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATG
ATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGG
GATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATAT
TGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGAC
ACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGGTAATCTCGATACCTTTTTGTCAGAAAATTGATGCCTCC
TCGAACACTATATAGGTTGTTTCTTATCCTGTGAAATATAGAATGACGTTTTTAGCTGTGTCAATGTAATATTAGTAAACACTGTTTCAGGAGTCTGGGAAGTTTGGACAGATGATACTAAGACGTTCAGTGTTGAGATTAATCTCAATTATTTTGTAACAGGCTATGGAAAGCTTAATTCTGTTTGATACAGCATTCCA
TGTTTACAAAGAAATAAATGCCTGGAAAGAGTTATATTTCTTCCTATCATTTTACATTATAATCTGAGCTATAAAATGCTGTTCATTTTTCATGTCCTGTTAACAACCCAAAGAAATTATATTGGTAAATGTTCTATGATGGGTATATTTAGTAATTGTTTGACAAATTAATTGCTCTAGAGCACTTTTCAAGTTACTGG
TAGAATCAGGTAGTAATTGTTTGATAAGTTGGTCTAGAGCACTTTTCAAGCTGCTGGTAGAATCAGGTTCCTTCCAAATTTATTCATGAGCTGTTTCTGTTTGATATTTAATGTTCCATATGTGCTTATGCAGCTCTGTATGGATATATCCATATAAAGTAGAGATCGTGTCTCTTTTTGTTATCTGTGTTTCTGGCACT
GTTTTTTGGTATATTATTAACCCAATGTACTTGCATGCTTGGTTTGGTAATTGCTTTATACATCCTTCTTTTGGCTAAAATTCAGACGCTTAACTAAATTTATGAAATGCTAACACCAAATCCATGAATTTCAAAAGGTTCACAACTAAAATCTCTACATTTGTATTACAGTTTGGTATGATGTAGCCATGGAAGAACCG
TCCCAGGTTTGAATGTCATAAGAAACATACCTTAGATGAGCATGTTTGACACTGCAAATTCTATGCTTATTGGGTGACAGTTGAGCAAGATGTGGTAATAGAATTATTGCTATCCCAGTGACAAAAAATTCATATGAAATGTAACATATGTATAAGATGACAGGATGTTTGTCTTGCATGAGACATCAGTATTAACTATT
ATGTGGTTTTGCACATTAATGAATGGACTACTGAGTGAACATGGGGGAAAGGATTAAGTATCGAAACATCCTTTTGGATTTAAGTCTGACTGCATGGGTATTGACTCAATGAGCCAGGGATGAAGGATAAGGATGTACAGACAATGGGACAAATATTTCCAAGTACAGACCCCAAATGTAAAGTCTGTCACTGGGGGCTT
GACTGGTTTGTAAAGCAGGCTTGGCGATATTCTCTCCACCAGTTATCAATTTGCTCTAAAGTGAAGTGACATATTTAGCTCCTCCAAAAAGAGGGCAG


>TRINITY_DN114846_c4_g1_i5 from sample A2 / Example unspliced isoform:
GCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCAGATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCC
TCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATGCAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCTCTTCCAGAGTTGTATT
TTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAATCCGATGGAAGACGATG
ACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTA
GCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGATGGGGGAGCGCGAGGA
TGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGCATGGTGATGAATGGCG
TGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTGCAAGAAGAGAATGGATGGGATAGTGACAGCGAAGATGATTTGCAAATT
GTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACC
AGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGC
ATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCTTCCTCCTAACAAGACA
GTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGA
TCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGG
ATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGgtaaatgaagctttctcttgtgatggcatctcaattttctagaaaatggtgttttgagctgtatctcaagctcttgagaggcatttgcagttgttgatgctcttatttatctcatgatctttcaaccaaattattttgtttttggcatgtgatcag


ATTGTCTTACGAGATGCATCAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCAT
ATCTGGAGGTGGAATGCTACCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGT
ACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAAT
TCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAG
GGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGA
AAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGAAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGAT
ATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTT
TAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATA
GAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAA
AGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAG
AAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAAC
GTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTAT
GATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGCCTTCGGAGAGAAGCACTGAAGACCAAGAATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAAC
GCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTCAGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGA
ATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAGTGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCA
GATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACCCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGATGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAG
CTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGATTGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATG
CAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTTCAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTA
GACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATGCAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCC
AACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATG
TATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCATTCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTG
ATTTAGG



Intron 7 GTACGATTCA ... GTCTGGCTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A111.02109207
A214.97711015
A31585228
A416.6745810
A518.7539810
A65.26910
A733.33101
A80500
A933.33220
A1018.521123



>TRINITY_DN113159_c0_g1_i6 from sample A3 / Example spliced isoform
GGAACTTTTAAAATCCCACTCCAACGATATTTCCAAATACTACCGAGCCCACTTTGCCGAAAATGAGGTTGTTACTCCTATGTATTTCCAAATACTACTACATTCGAATAATAATTAGAAGTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCA
GATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATG
CAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGGGTTTGGCTCTTCCAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTT
CGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGTGGAGAGCGCAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCC
TGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCCCTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATG
AACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTTGAGAATACAGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGG
GAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGACGATATTCCGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAA
CGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATGGATGGGATAGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAG
GAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAAGGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCA
GGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTCAAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGA
TTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAAATGGCTTTGAATTTTCTCTTCCTCCTAACAAGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAA
CGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTCGTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACA
CAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAGGTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATC
AGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCAAAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTAC
CTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCAGGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCA
AATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATGGTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAG
CAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTCACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAG
GTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCCAGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAAC
CACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGAGAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGG
TTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCGGCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAG
ATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATTACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAA
AAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGATAGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGG
TCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACGAACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGA
CATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGAGAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGG
GTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGATCGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGC
TTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGGAAAGCTTTCCTATATAATACCAGAACTATATTGATGTCTATTCATCAAATCGTATGATATGGAGGAAATCCCGCCTTCGGAGAGAAGCACTGAAGACCAAGA
ATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTCGCTCCAAATTGGAACGTTGGGCCAGTC
AGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAGCAAGATGACCATACTGCAAATCAGGAG
TGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACCCAAAAGAAGGCTGAGAATGAAACTCTTTTAGAAAATGAGACAGA
TGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGGTTCATATTAGGCAAACAATTTATTGAT
TGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCTTGATGTTAAGAATATAGTGCATGTCTT
CAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAACAATCTGTATGGGAGCAGAGGCACCATG
CAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACATTGTAAGACTCGGGAGAAAGAAGTTTC
TCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATCTTTCCCATTAATAACATATAAATTCAT
TCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGGCTAC


>TRINITY_DN113159_c0_g1_i2 from sample A3 / Example unspliced isoform:
GGAACTTTTAAAATCCCACTCCAACGATATTTCCAAATACTACCGAGCCCACTTTGCCGAAAATGAGGTTGTTACTCCTATGTATTTCCAAATACTACTACATTCGAATAATAATTAGAAGTCCATTACACTCACAGCTTCTACAACGTATTCTAAAAGTCGGGGATGAAGATGTTCGAGCAGTTAAGGGTAGCGGCTCA
GATAAAAAAGTCAAGAAGGCGGAAGGATTTCCCTGATAGTTTGGGTGGGAGTTAATTGCTTGTTTGTTTGCAGATATACGATTTTAATGAAAGGTTAAAAATAGGCGGCTTTCATCTCGCCTGCGTCGGCCAGGCCTCCTCGCTCATTCTATTCTCTATTCTCTATTTGCAAGGGAACAAAAAAAAGGCCAATCAAAATG
CAAAGCGTGTGAGCAAGAAGAGGTCTGATCCCCTCTGGATCTTCTGGAATTGAAACTCTCCATGGCCATTGCCCTCGAAAATCAAGGGTCGAGGGTTCCAATCTGGACAAGgtacgattcatctcgtcgtcggcttacaattttttttatatttttcgtattttctttaccatccaggttttgtctgattcatctcgatt
gtctggctagGGTTTGGCTCTTCCAGAGTTGTATTTTTTCATATATATATAATAACGGATGCTCCGTTCCAGAAAGGGAAATAAGAATGTTCTGACTTTCGCTTGATCGGAGAAAATAGTCAAGAGAGAGCGCAGAGGATAGTTTTCCTGTATCTGGAAAAATTATTATAAAGACTCGAGGGTTATCGTATCGGCTCTGT
GGAGAGCGCAAATATAAATCCGATGGAAGACGATGACGATTTCGGAGAACTGTATGAGGAGGAAGGTTTAGTGGAAAATTCGAGTTTTCTACCAATTCCTGTGCCTGTGAGATCTCAGGTTACTTACGAAGAAGAGTTCGATGACGATGAAGAGGTTTTATATGGGAGACCCTCACCATCGTCGTCGCAGCAGCAGGCCC
CTAGGATGCCTTCGTTGCCCCAGCAGGAGTCAGTAGCGGAGGGTTTTGATTTATCGCGGAACGACGCACATGAAGACACGGAACCTGGTGGAGACAATGAACCTGATGGGGTCAAAGAAGAGTCTGAGATGCAGTGGAATTATTCAGAAAACGATGAAGTCGGGGTTCATGCTGAAGCTTCCGTTCAAGGCGATTTTGTT
GAGAATACAGAGATCAAGATGGGGGAGCGCGAGGATGAATTGAATAAAGATAATGCTGAAAACCCAGTCAGTGAGCCAGTGGCGTGCGAAACGGACAGGGAAGAGAAAGCCGGCCGAGATTCGCCATTTGCACCAACTGAGCCGCCACTCCGCGAAAATGTGTTCCACTTTGAAGAGGAGAATGTTCTGCCGCAGCACGA
CGATATTCCGGGTCTTGGCATGGTGATGAATGGCGTGACAACCTCAATTCCTGGGCTTTCGAGAGGTTATGATGATATAGAAGAAGATGGAGAAGGCAACGGAGAGAACTGGGTTGCTGAAGGATCATCTGCGAGGCAGGATTTGACACCTCCTGCGGAATTGGCGGTTGCAGAGAATCAAGGTTTACAAGAAGAGAATG
GATGGGATAGTGACAGCGAAGATGATTTGCAAATTGTCCTCAATGATGATCGAGCGGTTTATCCCAATATGGAGAGAAGAGAATGGGATTTTGAGGAAGGAAGCGAGGATGAAGACGGGGATGATTTGGTGATTGTTGCGGGTGGTGATAATCCCATCGAGGATCAAGAATGGGGAGCTGAAGCCGCTTTACCTTTTGAA
GGTGCAGTATCTGCTCCTGCCGACAAGGTTTCACCAGCAGACAAAACTGGTGATGATAAGGGGCAAACAGGAAAAGGTAATGTTGGAATTGGAAATGCAGGTGTTCCAAGGGTTGGCTATGGCAGTCAGCATTATCATCCTCACTATTCACAATATAAGTATGTGAGGCCAGGGGCAGGATCAATTCCTGGGGGAATTTC
AAGTGCTTCTGGTGGTGCCCAAAATCACACTAGGCATTCTATCTCCAGTGGAGGTGTCTGGGCACCTGGCAAAGGTGTACCTACCACTGGAAGAGGTGATTGGTCCTCTAGTGGTGGCAGAGGGTCCGTGCAGAGAAATTTTCTCTCTGGGGCTGGCATGCCTCCTTGGAGCAGTGTGCCTGGAGGGCGTGGTTTTCCAA
ATGGCTTTGAATTTTCTCTTCCTCCTAACAAGTAAGTAATAGGTGATTTTTATATATTGTACCATAATAATATCATGCATTTTGACATCAAACTTTAGAGGGAGTGGTTGTAGAGGTTGGGATTGTAAAATGTGGATCAGTAATATAGGACAACAAAATGTTCCTCTGGGAAGAAGCCCTTTGGCTCTATGTAACCATGT
AAGTGCTTCTTAGCAGGTCTTGTAACTGTTAAAAATGTTATTTGAATGTATTAGTCTCTCTGTTGAAGGTGATGGCTAATTTTGGTATCTCCCATCAGGATCTCTTATAAATTATGCTGTTAATACATACACTAGCTTCAAAATATATGTGTAGTAGGAGGAAGCTTCGCTTTCATTTAAAAAGGCAGTCTCAACTATCA
AAAAATGTAGTTTGTTTTCTAGAGCACCGGTTGCAAATCTTGCCACAGGCCCACAACTGTGGATAATACCATGTTCGGTGACATCTTGATCAAGCAGCTGAAGTTTTGCACTTGACGGCAAAAGCAAGTTGATGAACTGATCCCGCTTGACGTTTGATTTTGCCAAGGCCTTTTTCTCTTGATTAGATTGGATTCTACAT
TGATGTCAGTGTAATATTTTATGAATTTTACTATCTCATATTGTAAAAGATTTGAGCTTCAGGCATCCTAGCAAGGTCCCTTGCTTCATCCTGGCACCATCCCTTTTCTGAACAATGCTATATTGGTATTTTTGGGGGGTCATTGTCAACAAATGGAAATAGCCTGGTCCTGGTCAGTAACTTTGTCAAGATGCCGGTTT
GTATACCAGAGAGCTGCTTCCATTCTTTGCCTTAATCAGGGCTCTGCAGATGGTTATTTCTTGTTGGTAACTTGGATATAAATGGGTGCTACACATGCATTTGCATTTAAGCGACTCTCATCATCATCCAGCGACAGTAGCACATCATTTGTGCACATGCTGACCTCTGGGAGAATAATATCAGATATTTTAGTGTGTGG
TTGCTGAAGGATATTTTTCTTCTGTGATACTGATGAGGTGTGTTGCCACTGCATTTGGCAAATGCAGGCATGTAAATGGAGTTGCTTTGAAATGTCAATTTGATTTGTTTAAAGACCATTTTTGGGAAGTTTGGACTTGTTTGAAGTGACTTGTTCAGTTTATGATCTTTAATTTACAATTGCTGCTTGCAGAGATGCTG
CTGCGTTATCAAAATTTCTTACTGTACATTGTTTAGTGACCTTTTCTATAGAGTTTGTGATGCTTGCAAAATCACATATTTATATTAAATTTCAATTGATACACATAATGCAAGTTTCAAACTTCAAAGGTTTGATATTTCTATGGGTTATTCCATGCCAACGTTGCCAACTTACCAAGATAGCAGGTTGAAGGTGTTGA
TTATCCATTTTTCCCTCGATGCTCACTCTGCAGGACAGTATTTGACATTGATGTTGATAACTTTGAGGAGAAGCCATGGAGGCTTACTACAATTGATACAACGGATTTCTTTAACTTTGGTCTTGATGAAGACAGTTGGAAGGAGTATTGCAGAAAACTGGAACAACTTAAGTTGGAGGCAACCATGCAAAGCAAAATTC
GTGTCTATGAAAGTGGACGTTCTGAGCAGGAGTATGATCCGGACCTACCAATTGAGCTGGCAACCGCCACAGGCCGGAATGAAGCTTATGGAGAAAGTACACAGCATAAACTGTCTGATCCGGGATCTGCAGCTTCAGCTGGTCGTGGAAGGGGTTTGGGACGTGGAAGAGCAATACTTCCAACTGGTAGAGCTATTCAG
GTTGAAGGTGGTTTTGGTGAAAGGCGTCCTTCAGTGGATTTTAGACGGCCACGCTTTCATGATTCTGATGCTGTAATAGAGATTGTCTTACGAGATGCATCAGAGGACGATACCATTGCCCCTTTGAAAGCACCCGAGAGTGCAAATGATGTTAATGAGAGAGAGGAGTCAGAAAGGGGAGAGATTGAAATGGGATGCCA
AAAACAAGTTAACACAGAGTCTTCCTTGCAAACACGGCATAGGGAGTCATGGGAAAGGGATGATGTCAGTAAGAAAATCATATCTGGAGGTGGAATGCTACCTAGAACACAGCATCATGGAGATGGGATTCTACCCTTGCCACCTGGTCCTCCGATTCATTGTCAGCCAGCTTCTAGTGCAGGACCATTGTTCTCAGCCA
GGATACCTGATCCGCAACATGCTAGCAGATGGTCACAGTCGGTGAAACCGCACAAGGGTTTTCCTCAAATAGGAAATGAGTACAGCAATGGGAGCATTGCAAATCAAGATGTGAGAGGTGGTAGAGCTGACAGTTATCGGAAGGAAGTAATAAGGGATAGTGTGGAAAGAACAGAGATAATAGAAATAGGATGCACAATG
GTTGACACTGAGAGAGAGCCCACTCCAGATGTAAGGTGCACAAATGTTGAACAACTTACTGTGGATGAAGTGCAAACCAATTCAAGATCAGATGGTCAAAGCAAACCAAATTCAGAAGTGAAAGCTGACGAGGATACTATCGATGACGAGGGCTCGGACCATTCTGTAAAAAGGCAAAAATTAAGTTCACAAAATGAGTC
ACCGGCAACAGCAGCAGGCTATTCCCATCATTTCAAGGTATCAAAGAGTGGAAACAGTAAAGCAGAATCTGGAAATAGCAGGGGTTATTCCAAGGACCGAGGTAATAGCAGGGACTATTCTAGAGGTCGTGGAAATGGTGAGGAAGAGATGTCACAGTACAGACCTCCAAAAAGGCTGGCAAAGGAAGGAAACAAACTCC
AGTCTGAGGAAGAGCCTTACTATCAGAAGGATTACTTGTGGAACAGTAGACACAGTAATGAGAGAGACAGTAGGCATCAGAAAAGCAGAGAAAATCCAAACCACCATAGAGATGAACAGTATCTACATCATGGAGACCGAGAAGAAATGTACCGTAGGAGAGTTAAAGAAGACAGAACTGATAAGCCTAGGGAACGGGGA
GAATATGCTAAAGCTTGGCTGGGGAAAAATGAGGATATGCGGAGTCTAAGAGAGAAAGATGAAGATTCACGGAGGAGAGATATCCATCCAAAGGAGGATGGTTTGCAACACAGGGACAGGGGACATGATACTGAAAGAGATGATAGGCAAGAGCTTTTGAATGTAAGAAAGAAGAAGGATGAAGATGATTGGAGACCTCG
GCTTGATAAAGAAGACATCAAGCGCGGCGAGAGAGAGGATAATATTCCAGCACGGCCAGAAAAAATGGATGATCCTTACTTTAGAAGAAAGAGAGATGAAGATCTCCTTTGGAAAGATAGAGCAGAGAAGGTTGAAATAGCACGGGATATGAGAGAAAGAGAGGATATGGGTGGAGAAAAACATGAGAAGGCTGATGATT
ACCGCAGGAGGAGAAAGGAAGAAGACTTGCGAAGAAGAGAAAAAATGGATAAAGATCAAAGCATTCGGGATCTCAGAGATAGAGAAGATATGGGTAGAGAAAAGCATGAAAGGGATGATGGCTACCTGCGAAGAAAGGATGAAGACTTGTCTAGGAGAGAGAGATCAGACAAGGATCAAGGCATACGGGATCTCAGAGAT
AGAGAAGACATGCATAGAGAAAAACACGAGAGGGATGATGATAGCCTTAGACGAAAGGAAGAGGACTTACGTAGGAGAGAAAGAGCAGATAAAGATGAAGGTCTTCGGGATCTAAGAGAGAGAGAAGATATGAGCAGGGAAAAACAGGAGAGGGATGATGATCACCACCTTAGACGAAGGGAGGAAGAGTCCCGAAGACG
AACTTTGTTTGATGACTCCCGGGCAAGAAAACAGAGGGATGAGTCCTGGAGACGAAGGGAAAGAGAAGATGAACATTGGAGAAAGCCAGGGTTTGAAGAGACATATGGTAGGAGAGATAGAGAGGAGGACAAGTCAATGCTTGGAGGTAGGAGCAGGCATCATTCTGAAGAAAAAATGTGGCATGGTAGTGACAGGGAGA
GAGGTCGCAGCAGAGAAGAAGCTAGTCTCTCTAATATTGATAAGGATCGCTTGAAAGAGAAAAGGAGACACGAAGAGCAACGTGGCAAGGTTAAAGACAGGGTGGATGATACATCAAGATCTAGACACCATGAACGAGATGACATGTATTCTTCTAGGATAGATCGCTTGGCTTATGATGAAAGAAGTTCACGGCTTGAT
CGTTCACATCTTCGATATGATCATTCAGGCAGTGGACACAGTGGAGAAGACCAGAAATTGCACTGGGAGAAACGATCCTATGATCATCGTAATGGTTCAGCTTCTGGCTTGGTTGATGACAGAGTTCGGCATGACCATGCATTAACCAGTAGGAGGAGGAAGACTGAAGATTATCACACTATGCATCATGAAAAAAAGCC
TTCGGAGAGAAGCACTGAAGACCAAGAATCGGCAAGTGTTTGGGGTGGCCATGCTGATCGTAGAACCCGCAACCACACAACGCCTCCAGTTCTTTCCAAAAGGATGCATGGGCAGGAAATGACAGAGCGCCATGCTTCAAAATTGAAACGAGTTGCTGCTGATGACAGTGCTTCTGAGGAAGAACATGTCAAAAGAGGTC
GCTCCAAATTGGAACGTTGGGCCAGTCAGAAAGAAAGGGACGATGCTAATAAATGGGACAATGATGCAATCAAACATCGGAATACACCTATCAATCATGAAGAATCTGCAGCTGGAAAAGAAGAGCAAACAAAGATGGAATGGAGTGTTGGTACAGAGTTGAAAAGGAAACCTGGAAAGGAGCGTCCGCATTCAAGAGAG
CAAGATGACCATACTGCAAATCAGGAGTGCAAGGAGGCTGTTTCGAGTGAGCCTGTTCATGTTGCTGATGGTACTCGGTCAGATGCAGATCAGAGTGGAAACCGACAACATTTTGAGACAGTAGCTAAATTGGAAAAGCGTAGGGAGCGTTTTAAACAACCGATTCCAAATGAAAAGGAAACCCAAAAGAAGGCTGAGAA
TGAAACTCTTTTAGAAAATGAGACAGATGAAGTTAAACAAGAAAGGCCTGCTCGAAAAAGGAGGTGGGGTGGCAGTAGCAGCTAAACTTGTCTGATTTACGTCTTCTTGAACAATGCATGATTTGCATGGTTTTTGATCGGTGAATTTAGAATTGCTTTTATTACAATGCCTCTCTGGAAGGTCTTTGACCAGGGAAAGG
TTCATATTAGGCAAACAATTTATTGATTGCCATGGGAGGTTGAAGTTTATATTTAGGGGCAAGAATGCACTTCTGCAAATGCAGGAGCTTGTGTAATCATTAAAATTGAAAATGTTATGGTGGATGAATTGGCTTCCAGGCTGCTGGACTAGTCATACTGAACTTAAATGTTATTGATATTTTCAGTACTGATCTTTCCT
TGATGTTAAGAATATAGTGCATGTCTTCAAAGAGCAGTATTTTCCATGAAACTGCAAAGAGTTTTTGTTCAGTTGATTTTAGACACTGGCATTGTTTATATGATCCAGGGCACTCCCTTTAGGGTGAGTGTATATAAGCTGAGTGGATCACACTGTCTCTGTGGCCTTGGGCAACAGCCTTTTTGTTGTCAAAATCCAAC
AATCTGTATGGGAGCAGAGGCACCATGCAGACAGACCCAAATTTAATTTTCGACATATGCTGTATATTTGGATTCAAATCCAACTTGTTCACAATGTATGCAAGCATGGAAGAGTATACTACCTTATTGAAGGTATATGACAATAAGGTGCAGATGTACCCTCTATTAGGGGCAAAAGCTAGTGAAAAGGCAGAGAAACA
TTGTAAGACTCGGGAGAAAGAAGTTTCTCTGTGAATTGGATAAGACTTATGCATCGTTTATTTCTTTGTACTTTGAGAATGTATGATGGACATTAGCTAGCAGGCTCTGTAAATTAATCTCTCGATTGTCCTCAAAACACGGGAGTTACATATAAATAGCTGAACTAGAAGGCAATGGTTAGCTACCTGCAGTTTATATC
TTTCCCATTAATAACATATAAATTCATTCTTTCTCTCATGTACTTTTTGCACGATTGGCAACATTTGAATAAAGAATGCTGATTTAGGGCTAC