sp|Q94AQ6|SIR4_ARATH ----> NAD-dependent protein deacylase SRT2

Intron 1 GTAACTGGAT ... TATCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	5.63	151	0	9
A2	10.28	96	0	11
A3	9.92	109	0	12
A4	17.17	82	1	17
A5	19.35	25	0	6
A6	17.37	295	0	62
A7	10.22	448	0	51
A8	11.99	470	2	64
A9	13.96	425.5	0	69
A10	13.55	370	0	58

>TRINITY_DN45675_c0_g1_i16 from sample A8 / Example spliced isoform

AGACCGGAGCTATGAACTTCCAGCTGGAAAAATAGAAAAATAGTTGGTGAGTCCCAAATTGTCCTAAATCCCCGAATTATCCTTCTGATTTCGTCCTTAATCAACTATAGGTTCCTCTCACTATCCCTGTTGCGGGTGATTCAATTAGAGCGTCTTCTCCTTCTGGGACTTTAGCCAGCCAGAGAGGTCTCCCTCTGTGT
TTTGCCAAAGGGAACCCTTAGTATAGCTCTATCTTTCTGTGGTGCCAGATAGTTGGATCCCCGGCCTCCTCCTGTGGCGGATAGATAGCCTTATTCCTTCTCGTTGGCTAGCTCTTTGGCAGTGATATAGACAGGTTCTTGAAGGTACCCCAAGACAGGTCGTTCGTATGGTACCCGAAGATCTTCATTCATTAATTGCG
ACTGAGGTGTTGTTGGATGGACGATTGGGCGCTTGGACGGATGGTTGGGCAGTAGAAAAGCGCTGGATAGAGGACGCGGAGCAGTTCATTCATTAATTGCGATTGCGATCGAGGTGTTGTTGGACGGATGGCGAAGTATGGCGGTCAGGGCGAGAACAAGGAACGCACAGACCGGAGCTATGAACTTCCAGCTGGAAAAA
TAGAAAAATAGTTGGAGTGCCACCGAGCTATCAAATGATAACATTGGAAGCATTCACAAGAGCAGGCTGCATTGCGTACTGCTGCAAGGGAGGGCAGTCTTTGCACACCTTCAGTTACAGTTGTAGCAGCAGCAGATCTTGAATCGGAGGAAGTCTCATTCCTCAAGGAACAGATTGCTTCATACTTTACAAACAAGCTT
CTGATGATTGTCCGTGATATACTCTGTCATATTACTGCTAAAGGAAGAAAAGAGGATGCTTCTGTAGAGGCTGTTTCCCACGAGTTCCCCAAATTGGCTGGTTCTCACTTACCAATGGGTGATGGTGCCTTGGAATTCATTAAGCATGTTTGTGCCGTTCTTGCTCTTGATCAACACGTTCAACATGATGTTTTGGTATC
AAACCCACCAATGTATGAAGTGCAGTTTGAACATCATTCACAGCAGCTATCATCTTTTTAGTATCTACAGTATTTGGGCTTTTCTCAGACTGAGTTAACACTGCCTACAAACCTCAATCAACCTTTGTCATATTCAGGGGATCATAAAGCAGCAATAGTGTAACACATTAACCATGTGAACAGCCAGAAAGGGTGATGAT
ACTCCGCTAGAAATCAATTACCACTGCAGTCCAAACTCCATAATGATCTCTGAAATTGTTGTATCTTTTGGATCCTAGACTGGACTTCGTGCACTGACTGCTTTTATATATTGAAGATGAAGTCTTTAATGCTGGTCATAAGCAGCCCTCTTGCAGCAGAACCTATTCCGGAACTATTTTTTTCATGAAAAAATTCAGAT
ATTCTTATATATGCCAGTTATAACATGGTGTATTTCCGACTGTAATGGCACCCTTCTATTGTTGGAACAAGTCTGAAAAACACCTGGGGCCTTATTAATATGCTATATTTTATCTCAATCATCTCCCCATTAGTTTATTTACTGCTATTAATACATCAATCCATTTGTATGAAGGTGTTGTTCCAATGCAAATCCCTTTG
CTTTGTCGTAAAATCTCTTTTATTTGTGCATCAACAGTCCGAAGAAATTTTAGTGATGGCATAACTTCATATGACTCAAACATTGGAACACACCCTATATACAATGTAAATGCCTTTGAATTGTGTGCATTCATCAATCTGAAAAGTCTGTGCTGTATCCTTCATCTTG

>TRINITY_DN45675_c0_g1_i14 from sample A8 / Example unspliced isoform:

AAGTCTCATTCCTCAAGGAACAGgtaactggatgaaaaaaatgtctttcttgctggcatttatttcatttaactataaataataaatggagggtgctaaggttgtgttgaatcatcatgactttttctgaatttatcttgcagATTGCTTCATACTTTACAAACAAGCTTCTGATGATTGTCCGTGATATACTCTGTCAT
ATTACTGCTAAAGGAAGAAAAGAGGATGCTTCTGTAGAGGCTGTTTCCCACGAGTTCCCCAAATTGGCTGGTTCTCACTTACCAATGGGTGATGGTGCCTTGGAATTCATTAAGCATGTTTGTGCCGTTCTTGCTCTTGATCAACACGTTCAACATGATGTTTTGGTATCAAACCCACCAATGTATGAAGTGCAGTTTGA
ACATCATTCACAGCAGCTATCATCTTTTTAGTATCTACAGTATTTGGGCTTTTCTCAGACTGAGTTAACACTGCTTACAAACCTCAATCAACCTTTGTCATATTCAGGGGATCATAAAGCAGCAATAGTGTAACAC

Intron 2 GTCTTTCTCA ... CTCTTATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.29	22.3	17	4.4
A2	49.56	15.4	15	6.2
A3	36.34	14.1	8	5.5
A4	43.32	19.6	15	2.4
A5	66.91	5.5	11	0.6
A6	32.49	60.5	29	5.5
A7	32.36	46.1	22	6.8
A8	31.62	54.2	25	9.7
A9	47.67	35.4	32	5.9
A10	27.11	24.3	9	0.5

>TRINITY_DN119004_c1_g1_i3 from sample A1 / Example spliced isoform

CATACCTACATCTCCTTCCTCTTCCTCAGACTAGTGGGTATTTATTTGGTTGGAAGTTGGAACATTTTGCAGCATTTTCCACTGCAGCACTTGGCAGGAGTGTTGTGCCAAACGAGGACCAGTCGTCTCCACGTTTTATTCTATTCAAATCTGCACAAAGCTCAAAGAGAGAAAGGGCGCAGCAGGATCAACGACAACCA
CAATGGCGATGGCAATAACAACACATCATTCATTGTTTCCGTCATTACGAAGGAGCATATATGCATTTTTGCAAGGATTGAAATACAACAAAGCTTTACCAGGGCAACATCTTCAAGGGATGCATTTTTATTTAGTCCAAAAGCATGGGCACCTTCATTCAGAAATGTGTAACAGAGGCTTTTCCTTTCCAAATGCTGGA
GGTCATGTGTTGAGAAAATGCATGAGTGCTGTAGTGGACACAAGAACAGAAGATCAAAGAGAAACACCTTGGAAATTCCTAAAAGATAAGAAATTAGTTCCTGAATGTGCTCCTCCAAGTGATCGAGATCTTGGTCTCCTATATAATTTTGTCCAAGAGAGGGGTGCAGTCGAAAGCTTGTGGTGTTGACAGGAGCAGGC
ACAAGTACTGAATGTGGAATACCTGATTATAGAAGTCCACATGGAGCTTATAGTTCTGGATTCAAGCCTATTACCCATCAAGAATTTGTTCGGTCAGCCAGGACCCAGCGGAGATATTGGGCTAGGAGTTATGCTGGTTGGCAGAGGTTTGTTGCTGCACAGCCAGGGTCAGCACACAAGGCACTTGCATCTCTTGAAGC
AAAGGACCGGGTATATGGAATGGTTACTCAAAATGTGGACAGGCTTCATAATAGAGCAGGCAGCAATCCTATTGAGTTGCATGGAACAGTATATTCTGTCATATGTTTGGAGTGTGGTTCCATCACAGGTCGACACTTATTCCAAGATCGAGTGAAGGCTTTAAATCCGGAGGTGAAACACATGGGCCTTTTTTGATGTT
ATGTTCTATTGTTGTGTGTGTATTTATGTTTGAGCATAGCCTTTAATAAATCTGTGTATATA

>TRINITY_DN119004_c1_g1_i8 from sample A1 / Example unspliced isoform:

CATACCTACATCTCCTTCCTCTTCCTCAGACTAGTGGGTATTTATTTGGTTGGAAGTTGGAACATTTTGCAGCATTTTCCACTGCAGCACTTGGCAGGAGTGTTGTGCCAAACGAGGACCAGTCGTCTCCACGTTTTATTCTATTCAAATCTGCACAAAGCTCAAAGAGAGAAAGGGCGCAGCAGGATCAACGACAACCA
CAATGGCGATGGCAATAACAACACATCATTCATTGTTTCCGTCATTACGAAGGAGCATATATGCATTTTTGCAAGGATTGAAATACAACAAAGCTTTACCAGGGCAACATCTTCAAGGGATGCATTTTTATTTAGTCCAAAAGCATGGGCACCTTCATTCAGAAATGTGTAACAGAGGCTTTTCCTTTCCAAATGCTGGA
GGTCATGTGTTGAGAAAATGCATGAGTGCTGTAGTGGACACAAGAACAGAAGATCAAAGAGAAACACCTTGGAAATTCCTAAAAGATAAGAAATTAGTTCCTGAATGTGCTCCTCCAAGTGATCGAGATCTTGGTCTCCTATATAATTTTGTCCAAGAGAGgtctttctcaattactggaaattttgccatcaatgtttc
tgatatcattactaggcttcagttcttgttgtgtttttgttcatgatggcacattgagtgggttataccatcccattgtacagtagtatacctcaatctaatgcaagggtatttaacagtatcttttagcacagcacatatgaacatactttaatattaattttgattcttctcttattagGGGTGCAGTCGAAAGCTTG
TGGTGTTGACAGGAGCAGGCACAAGTACTGAATGTGGAATACCTGATTATAGAAGTCCACATGGAGCTTATAGTTCTGGATTCAAGCCTATTACCCATCAGGAATTTGTTCGGTCAGCCAGGACCCAGCGGAGATATTGGGCTAGGAGTTATGCTGGTTGGCAGAGGTTTGTTGCTGCACAGCCAGGGTCAGCACACAAG
GCACTTGCATCTCTTGAAGCAAAGGACCGGGTATATGGAATGGTTACTCAAAATGTGGACAGGCTTCATAATAGAGCAGGCAGCAATCCTATTGAGTTGCATGGAACAGTATATTCTGTCATATGTTTGGAGTGTGGTTCCATCACAGGTCGACACTTATTCCAAGATCGAGTGAAGGCTTTAAATCCGGAGTGGGCTTT
AGGAATCGAAAGTCTGGAAAAATGTTCTCCTGGTTCAGATGCAAGTTTTGGCATGCAGCAACGACCTGATGGAGATATTGAAATAGATGAGAAATTTTGGGAGAACAATTTTACTATTCCAACCTGTGAGCAATGTGGTGGTGTTCTAAAGCCTAATTCCACATGAAGCTTATAGTTCTGGATTCAAGCCTATTACCCAT
CAGCTAAGCTTATTATCCTTAACTGTGACAAAGTGGTCTCCTGGAACACTGCAACGTAGATTTTCAAATACTTAGAAGAAAAGTGG

Intron 3 GTCTTTCTCA ... AGGGGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.74	21	17	4.3
A2	51.78	14	15	5.5
A3	40.1	12	8	5.3
A4	41.67	21	15	0.3
A5	68.75	5	11	0.8
A6	35.59	52.5	29	9.8
A7	32.59	45.5	22	7
A8	32.68	51.5	25	13.5
A9	45.39	38.5	32	8.8
A10	26.09	25.5	9	1

>TRINITY_DN114157_c0_g1_i6 from sample A2 / Example spliced isoform

CACATGGAGCACATACCTACATCTCCTTCCTCTTCCTCAGACTAGTGGGTATTTATTTGGTTGGAAGTTGGAACATTTTGCAGCATTTTCCACTGCAGCACTTGGCAGGAGTGTTGTGCCAAACGAGGACCAGTCGTCTCCACGTTTTATTCTATTCAAATCTGCACAAAGCTCAAAGAGAGAAAGGGCGCAGCAGGATC
AACGACAACCACAATGGCGATGGCAATAACAACACATCATTCATTGTTTCCGTCATTACGAAGGAGCATATATGCATTTTTGCAAGGATTGAAATACAACAAAGCTTTACCAGGGCAACATCTTCAAGGGATGCATTTTTATTTAGTCCAAAAGCATGGGCACCTTCATTCAGAAATGTGTAACAGAGGCTTTTCCTTTC
CAAATGCTGGAGGTCATGTGTTGAGAAAATGCATGAGTGCTGTAGTGGACACAAGAACAGAAGATCAAAGAGAAACACCTTGGAAATTCCTAAAAGATAAGAAATTAGTTCCTGAATGTGCTCCTCCAAGTGATCGAGATCTTGGTCTCCTATATAATTTTGTCCAAGAGAGTCGAAAGCTTGTGGTGTTGACAGGAGCA
GGCACAAGTACTGAATGTGGAATACCTGATTATAGAAGTCCACATGGAGCTTATAGTTCTGGATTCAAGCCTATTACCCATCAGGTGAGTTTGGTGCACACAAAATTTACTGCTTCATGGAATTATCTTATCAAACTAATAGCTTATCCTCTGTATTTGGAAAGGAATACTGTTCTTGATTCAA

>TRINITY_DN114157_c0_g1_i11 from sample A2 / Example unspliced isoform:

CACATGGAGCACATACCTACATCTCCTTCCTCTTCCTCAGACTAGTGGGTATTTATTTGGTTGGAAGTTGGAACATTTTGCAGCATTTTCCACTGCAGCACTTGGCAGGAGTGTTGTGCCAAACGAGGACCAGTCGTCTCCACGTTTTATTCTATTCAAATCTGCACAAAGCTCAAAGAGAGAAAGGGCGCAGCAGGATC
AACGACAACCACAATGGCGATGGCAATAACAACACATCATTCATTGTTTCCGTCATTACGAAGGAGCATATATGCATTTTTGCAAGGATTGAAATACAACAAAGCTTTACCAGGGCAACATCTTCAAGGGATGCATTTTTATTTAGTCCAAAAGCATGGGCACCTTCATTCAGAAATGTGTAACAGAGGCTTTTCCTTTC
CAAATGCTGGAGGTCATGTGTTGAGAAAATGCATGAGTGCTGTAGTGGACACAAGAACAGAAGATCAAAGAGAAACACCTTGGAAATTCCTAAAAGATAAGAAATTAGTTCCTGAATGTGCTCCTCCAAGTGATCGAGATCTTGGTCTCCTATATAATTTTGTCCAAGAGAGgtctttctcaattactggaaattttgcc
atcaatgtttctgatatcattactaggcttcagttcttgttgtgtttttgttcatgatggcacattgagtgggttataccatcccattgtacagtagtatacctcaatctaatgcaagggtatttaacagtatcttttagcacagcacatatgaacatactttaatattaattttgattcttctcttattaggggtgcag


TCGAAAGCTTGTGGTGTTGACAGGAGCAGGCACAAGTACTGAATGTGGAATACCTGATTATAGAAGGTTGTATCTTAATCTACCTTTCCTCCGAATTGCAGGCCATCAAGTAACTTGAAAGTTGAAAGCATCTGTAATCAAGCAATGATAGCATAGCTAAACTAAACAGTGACCTTGTTTGAACTTATGCTTGTTGTGGT
TAGACATGATTGATTTTCAAAACCCATGTTACTCGTACAAAGTCTGGATCTAAAGAGTCTGCTGAACTTTTAATAGCAACCTGCATTTTGCTGCATGCTTTGTATCCCACTAGAAATGCAGCATGCCTTTTCTTCAATTCATTTTTAGTTCCAGATGAGATTTCTTTCATTCTTCTATTGTCACAAATATAATGTTCTCG
TTTCTGTTATTGTCTTATCAAATTGATGAACATATGTTATTAGAT

Intron 4 GTACATAAGA ... TCTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	3	0	0
A2	28.14	15.3	2	6
A3	33.33	2	2	1
A4	14.29	6	0	1
A5	44.44	1.3	2	1
A6	14.71	40.7	7	7
A7	21.3	40.7	3	11
A8	27.73	44.3	0	17
A9	22.8	40.7	1	12
A10	30.7	31.7	1	14

>TRINITY_DN44050_c1_g1_i7 from sample A6 / Example spliced isoform

CATGGACCACATACCTACATCTCCTTCCTCTTCCTCAGACTAGTGGGTATTTATTTGGTTGGAAGTTGGAACATTTTGCAGCATTTTCCACTGCAGCACTTGGCAGGAGTGTTGTGCCAAACGAGGACCAGTCGTCTCCACGTTTTATTCTATTCAAATCTGCACAAAGCTCAAAGAGAGAAAGGGCGCAGCAGGATCAA
CGACAACCACAATGGCGATGGCAATAACAACACATCATTCATTGTTTCCGTCATTACGAAGGAGCATATATGCATTTTTGCAAGGATTGAAATACAACAAAGCTTTACCAGGGCAACATCTTCAAGGGATGCATTTTTATTTAGTCCAAAAGCATGGGCACCTTCATTCAGAAATGTGTAACAGAGGCTTTTCCTTTCCA
AATGCTGGAGGTCATGTGTTGAGAAAATGCACGAGTGCTGTAGTGGACACAAGAACAGAAGATCAAAGAGAAACACCTTGGAAATTCCTAAAAGATAAGAAATTAGTTCCTGAATGTGCTCCTCCAAGTGATCGAGATCTTGGTCTCCTATATAATTTTGTCCAAGAGAGGGGTGCAGTCGAAAGCTTGTGGTGTTGACA
GGAGCAGGCACAAGTACTGAATGTGGAATACCTGATTATAGAAGTCCACATGGAGCTTATAGTTCTGGATTCAAGCCTATTACCCATCAGGAATTTGTTCGGTCAGCCAGGACCCAGCGGAGATATTGGGCTAGGAGTTATGCTGGTTGGCAGAGGTTTGTTGCTGCACAGCCAGGGTCAGCACACAAGGCACTTGCATC
TCTTGAAGCAAAGGACTGGGTATATGGAATGGTTACTCAAAATGTGGACAGGCTTCATAATAGAGCAGGCAGCAATCCTATTGAGTTGCATGGAACAGTATATTCTGTCATATGTTTGGAGTGTGGTTCCATCACAGGTCGACACTTATTCCAAGATCGAGTGAAGGCTTTAAATCCGGAGGGCGGCCAAAGAGAAGGGA
AATTCACCTATAGCAATTGTGAACATTGGTCCAACACGAGCTGATGACTTGGCATCCATAAAGATTGAGGCCCGCTGTGGTGAGATACTACCCAGATTGCTGGCCATGGGTTCCCTCGATATACCTCTCTAGTTGCCTAATTGAGGCATTCTGATTATTATCATTGTACTCGCCCAAAGCTCTTCTTTGATTATGTATTC
ATCTATCAAGTGACTATATCTTATCTTTGTACAGGACAATTTTTATGTAGAGATTAAGAACACCAATAATTGTATCGTAGCCTTTCCATAATAAATATATTTGAAATGTAACTATTCCATATTTAATCAAGTAGATTTTACTCAGCTATCCACATATGTGCTTTATTTTGAAATGAATTGGATAATTCAATTGTGTAAGA
GGGATCTAAATAGAAAAGCTTTAATCTAATCAAAATATGTAACATGGGTC

>TRINITY_DN44050_c1_g1_i10 from sample A6 / Example unspliced isoform:

TCCACATGGAGCTTATAGTTCTGGATTCAAGCCTATTACCCATCAGGGCGGCCAAAGAGAAGGGAAATTCACCTATAGCAATTGTGAACATTGGTCCAACACGAGCTGATGACTTGGCATCCATAAAGATTGAGGCCCGCTGTGGTGAGgtacataagacacaaatttggattggaccaaactgttcttgttttgtttgc
tttcctccgctgctgggcatttgcatttcagtttgaaacactatgtatgctttccagactagcatttctatatcatcatattaatgtttttcagcatacatctctggatgcaactcttttgcagATACTACCCAGATTGCTGGCCATGGGTTCCCTCGATATACCTCTCTAGTTGCCTAATTGAGGCATTCTGATTATTA
TCATTGTACTCGCCCAAAGCTCTTCTTTGATTATGTATTCATCTATCAAGTGACTATATCTTATCTTTGTACAGGACAATTTTTATGTAGAGATTAAGAACACCAATAATTGTATCGTAGCCTTTCCATAATAAATATATTTGAAATGTAACTATTCCATATTTAATCAAGTAGATTTTACTCAGCTATCCACATATGTG
CTTTATTTTGAAATGAATTGGATAATTCAATTGTGTAAGAGGGATCTAAATAGAAAAGCTTTAATCTAATCAAAATATGTAACATGGGTC