sp|Q76H85|H4_SILLA ----> Histone H4

Intron 1 GTAAGTTAGC ... ATTGTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.46	8	23	32
A2	65.12	15	25	31
A3	80.25	8	30	35
A4	54.72	24	40	18
A5	53.98	26	29	32
A6	62.45	349	515	646
A7	60.38	165	225	278
A8	66.21	186	301	428
A9	61.75	263	414	435
A10	60.73	161	259	239

>TRINITY_DN124484_c1_g2_i2 from sample A2 / Example spliced isoform

CGGGCTCATCTATGAAGAGACCAGGGGCGTCTTGAAGATATTTTTGGAGAATGTTATTAGAGATGCTGTGACTTACACAGAGCATGCCAAGAGAAAGACCGTAACAGCGATGGATGTCGTTTACTCCCTAAAAAGGCAGGGCCGAACCCTCTACGGCTTCGGAGGTTGAGCAGTCTCACTTTGATTGCTATGTATCATTC
AATCTGGTGTCTCTGGACACCACCTCTGTATTTGTGGAAATTTTTAAAACCAATTTGATACTGCTAAATGTTGATTCCGTCACTTTTCTTGAAGGCTGCAGTTGTTTGGTTTCAGTACTTCTTGCAATTCACAGGAGGCTGGAGCTAATAAGATGATCTCGGAGTTGTAGGCGAGGTTGCAGTTGCTATGTATCATTCCA
TTTGGTGTCTCTAAACACCACCACCACCTCTGTATTTGTTGAAAATTTTAAAACCTGTTTGGTACTGCTCAATGTGGATTCTGTTCCTTTACTTGAAGGTTGCAGTGATTTGCAAGTTAGCTGTATTCT

>TRINITY_DN124484_c1_g2_i3 from sample A2 / Example unspliced isoform:

CGGGCTCATCTATGAAGAGACCAGGGGCGTCTTGAAGATATTTTTGGAGAATGTTATTAGAGATGCTGTGACTTACACAGAGCATGCCAAGAGAAAGACCGTAACAGCGATGGATGTCGTTTACTCCCTAAAAAGGCAGGGCCGAACCCTCTACGGCTTCGGAGGTTGAGCAGTCTCACTTTGATTGCTATGTATCATTC
AATCTGGTGTCTCTGGACACCACCTCTGTATTTGTGGAAATTTTTAAAACCAATTTGATACTGCTAAATGTTGATTCCGTCACTTTTCTTGAAGGCTGCAGTTGTTTgtaagttagccgtattttattcaattagttttcgaggaagtgattgttcgtctagggttgtgagttaatttgtgtaagggtttcatcatttct
taaattgtgtcagGGTTTCAGTACTTCTTGCAATTCACAGGAGGCTGGAGCTAATAAGATGATCTCGGAGTTGTAGGCGAGGTTGCAGTTGCTATGTATCATTCCATTTGGTGTCTCTAAACACCACCACCACCTCTGTATTTGTTGAAAATTTTAAAACCTGTTTGGTACTGCTCAATGTGGATTCTGTTCCTTTACTT
GAAGGTTGCAGTGATTTGCAAGTTAGCTGTATTCT

Intron 2 GTGCATTTGC ... TTGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60	6	9	1
A2	69.23	8	18	15
A3	58.82	7	10	6
A4	55	9	11	7
A5	61.54	5	8	5
A6	47.32	216	194	25
A7	42.67	86	64	8
A8	44.1	161	127	23
A9	50.79	217	224	35
A10	34.62	102	54	3

>TRINITY_DN109005_c1_g1_i3 from sample A4 / Example spliced isoform

GGAGAAAGCAAGAATGTCTGGGCGTGGAAAGGGAGGAAAGGGTTTGGGGAAGGGAGGTGCCAAGAGGCACAGGAAGGTGCTCAGAGATAATATCCAGGGCATCACCAAGCCCGCTATCAGACGTCTCGCCAGGAGAGGAGGCGTCAAGCGCATCAGCGGCCTCATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCT
TGGAGAATGTGATTAGAGATGCCGTGACTTACACGGAGCACGCGAGGAGAAAGACTGTGACAGCCATGGATGTCGTTTACTCCCTAAAAAGGCAGGGCCGAACCCTCTATGGATTCGGAGGTTGAGCAGTCTCACTTTGATTGCTACATATCACTCCATCTGGTGTCTCTAGACACCACCTCTGTATTTGTGGAATTTTT
AAAACCTATTTGGTACTGCTGAATGTGGATTCCGTTGCTTTTCTTGAAGGCTGCAGCGATTTGCAAGTTAGCTGTATTCTATTCAATTATTTTCCGAGGAAGTGATTGTTCGTCTAGTGTGTGCTAGGGTTTCATTTTTTTTCTTCAATTAACAGCAGGTTGCAGCTAATACGATGATCATGGAGTTGCAGGTTGGAGCT
AATACGATGATCACGGAGTTGCAGGTGCACTTGCTTTGCTTTTATTGTGAAATCCTTTAACAGTTGATGCGACTCACTTGTAAAATCCGTGACCTCCTTAGAGCACTGAAGCACTCTAAAAGGTTTGCTTTTGCTTACCGTTTTGGTATTCTATTGGATTTTAGAATTGTTTTTTTCTTTCACTTCCTATTTGTTCAAAT
CCAGTGGAAACGGTTGAAATTGTAAAAAAAAGGTCAAGGAATGTAATTTTTCTTTTGTGCACTTATCGCTAGTTCAAATACAGTGGAAACGGTTAAAATTGCATACAAAAAAAGGTTCACAGAATCAAATAAAAAATGGACTGTCAAGGAATCAAATAAAAGAATGAGATAAGTATGAGACTTCAATTATTAAGAGGATT
AGATTTAATGATTTTAGTTGTAATTGCCCTAAAGTTAACACATATTAAGTTTTTAAAACTTGGATGGTTTGA

>TRINITY_DN109005_c1_g1_i5 from sample A4 / Example unspliced isoform:

GGAGAAAGCAAGAATGTCTGGGCGTGGAAAGGGAGGAAAGGGTTTGGGGAAGGGAGGTGCCAAGAGGCACAGGAAGGTGCTCAGAGATAATATCCAGGGCATCACCAAGCCCGCTATCAGACGTCTCGCCAGGAGAGGAGGCGTCAAGCGCATCAGCGGCCTCATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCT
TGGAGAATGTGATTAGAGATGCCGTGACTTACACGGAGCACGCGAGGAGAAAGACTGTGACAGCCATGGATGTCGTTTACTCCCTAAAAAGGCAGGGCCGAACCCTCTATGGATTCGGAGGTTGAGCAGTCTCACTTTGATTGCTATATATCACTCCATCTGGTGTCTCTAGACACCGCCTCTGTATTTGTGGAATTTTT
AAAACCTGTTTGGTACTGCTGAATGTGGATTCCGTTGCTTTTCTTGAAGGCTGCAGCGATTTGCAAGTTAGCTGTATTCTATTCAATTATTTTCCGAGGAAGTGATTGTTCGTCTAGTGTGTGCTAGGGTTTCATTTTTTTTTCTTCAATTGATAGCAGGTTGCAGCTAATACGATGATCATGGAGTTGCAGgtgcattt
gctttgcttttattgtgaaatctaatacgattattttcagaggaagtgattgttcgtctagtgtgagctagggtttcaattttttttcaattgattgcagGTTGGAGCTAATACGATGATCACGGAGTTGCAGGTGCACTTGCTTTGCTTTTATTGTGAAATCCTTTAACA