sp|Q9SGA2|GDPD1_ARATH ----> Glycerophosphodiester phosphodiesterase GDPD1, chloroplastic

Intron 1 GTGAGTATTA ... TGTTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.05	17	0	4
A2	20	24	0	6
A3	33.33	6	4	3
A4	30	7	1	3
A5	0	8	0	0
A6	25	30	1	10
A7	18.75	26	0	6
A8	15.22	39	0	7
A9	13.89	31	0	5
A10	16.67	5	1	1

>TRINITY_DN40811_c0_g1_i4 from sample A9 / Example spliced isoform

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAA
CAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTTCCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGATTGCATAATGCGAGGCGCA
TTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGAAAAGCCAATGATGGCAGTATTTG
CAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAAGGGCAGAAAAGTTTTTTATTCCA
GCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAGAGCAATTTTACAGAACCCTGGATTAGTC
TCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACATCCTCGGATGACATCCGCTCTAGGGATAT
ATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCATGCTACAATCTAGCAATGGCTTTTGCGTTGTCTTTGGCCCATCAATGTCTTTTTGCTTTGTTCTTACCCCTTTCCGCATCATGGGGAACATGTGTTGTTG
TTGGAATCCACGTGCCTGGATCCTCTTTAGTCTCGACTCTCTCATTTTACCTGATTGTGATACCGTGCGTTCCAACTTCCCCGCACCAATTTTCAAATAACAAAACAGGTAGACTGGTAGCTATAAAAAGCAATAACGTCCTTACACGTAATAATGGTCGTGTCCTGATTCTTGGGATAAGGATTGTTTGTTACGATAAC
GACTCACGAGTCACGAGACTCACGAGCATTACTTCTCGATGGAATCAACTTTAACATTACTCGGG

>TRINITY_DN40811_c0_g1_i6 from sample A9 / Example unspliced isoform:

CTACTCACTTACGGGGAACTTAAgtgagtattattccaataatctatctatcttgaattacacatccataattctaataagataaggttaaatattttgaaacagttctgaatttatcaagtagatcagcaataagtaagaaaaaaatatacatttagtcaagatttagtttgataaatagttcacaaattttgtgacaa
atgattgcgactcaactggaatttattcaaatttatgaggtgtgggaaatggccgttattgtattcatgaaattaatttcgtttcagatttatggttttggcttaatcccttaaaaatttaattgatgtggtgtgtgttctgcagTAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATA
GTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACATCCTCGGATGACATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCATGCTA
CAATCTAGCAATGGCTTTTGCGTTGTCTTTGGCCCATCAATGTCTTTTTGCTTTGTTCTTACCCCTTTCCGCATCATGGGGAACATGTGTTGTTGTTGGAATCCACGTGCCTGGATCCTCTTTAGTCTCGACTCTCTCATTTTACCTGATTGTGATACCGTGCGTTCCAACTTCCCCGCACCAATTTTCAAATAACAAAA
CAGGTAGACTGGTAGCTATAAAAAGCAATAACGTCCTTACACGTAATAATGGTCGTGTCCTGATTCTTGGGATAAGGATTGTTTGTTACGATAACGACTCACGAGTCACGAGACTCACGAGCATTACTTCTCGATGGAATCAACTTTAACATTACTCGGG

Intron 2 GTAATTTGCT ... TCTGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	2	0	7
A2	85	3	4	17
A3	73.91	6	1	17
A4	66.67	2	8	4
A5	57.14	3	0	4
A6	33.33	6	0	3
A7	40	3	0	2
A8	52.94	8	0	9
A9	62.5	6	1	10
A10	50	3	0	3

>TRINITY_DN40811_c0_g1_i5 from sample A9 / Example spliced isoform

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAA
CAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTTCCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGATTACACAATGCAAGGCGCA
TTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGAAAAGCCAATGATGGCAGTATTTGCAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTT
GATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAAGGGCAGAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGA
GAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAGAGCAATTTTACAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATG
GGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACATCCTCGGATGACATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTG
GCATGCTACAATCTAGCAATGGCTTTTGCGTTGTCTTTGGCCCATCAATGTCTTTTTGCTTTGTTCTTACCCCTTTCCGCATCATGGGGAACATGTGTTGTTGTTGGAATCCACGTGCCTGGATCCTCTTTAGTCTCGACTCTCTCATTTTACCTGATTGTGATACCGTGCGTTCCAACTTCCCCGCACCAATTTTCAAA
TAACAAAACAGGTAGACTGGTAGCTATAAAAAGCAATAACGTCCTTACACGTAATAATGGTCGTGTCCTGATTCTTGGGATAAGGATTGTTTGTTACGATAACGACTCACGAGTCACGAGACTCACGAGCATTACTTCTCGATGGAATCAACTTTAACATTACTCGGG

>TRINITY_DN40811_c0_g1_i7 from sample A9 / Example unspliced isoform:

TGCCTTCTTAGAACTTGGAGATTTGCTTTGTTTATAAAATGTTTAAGCTTGTTTTTGTCTTTAGCAGAAATTCTAAGGTTGTTTTAAATTTGCTTCAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGgtaatttgctttctatatttttgtttcctgtttcattcaaac
tgtattttgttcgaaagtctttcaagatgcaaaaaggaatataactttacctagggatcagagtgttgaagggttccattcttatgtaggtttatccatcttgcaggtttagggaccttgtgggactcggccaaatatcttatattttgcaactagaccgttctaacttgtcttttgttatgatccgtgtttcagggatt
gcataatgcgaggcgcattggacaactgggattcagcagactttcttttccaccaaaccgttctaactttcgtgatggtctgtctgtttcagGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGAAAAGCC
AATGATGGCAGTATTTGCAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAAGGGCAG
AAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAGAGCAATTTTAC
AGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACATCCTCGGATGAC
ATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCATGCTACAATCTAGCAATGGCTTTTGCGTTGTCTTTGGCCCATCAATGTCTTTTTGCTTTGTTCTTACCCCTTTCCGCATCATG
GGGAACATGTGTTGTTGTTGGAATCCACGTGCCTGGATCCTCTTTAGTCTCGACTCTCTCATTTTACCTGATTGTGATACCGTGCGTTCCAACTTCCCCGCACCAATTTTCAAATAACAAAACAGGTAGACTGGTAGCTATAAAAAGCAATAACGTCCTTACACGTAATAATGGTCGTGTCCTGATTCTTGGGATAAGGA
TTGTTTGTTACGATAACGACTCACGAGTCACGAGACTCACGAGCATTACTTCTCGATGGAATCAACTTTAACATTACTCGGG

Intron 3 GTAAGTTCTT ... CCAATAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	4	7	1
A2	42.31	15	11	11
A3	33.33	11	6	5
A4	48.72	10	8	11
A5	100	0	1	6
A6	22.22	7	3	1
A7	35.14	12	10	3
A8	25.93	10	5	2
A9	30.43	8	5	2
A10	38.46	4	2	3

>TRINITY_DN88635_c0_g1_i5 from sample A4 / Example spliced isoform

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAA
CAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTTCCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGATTGCATAATGCGAGGCGCA
TTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGAAAAGCCAATGATGGCAGTATTTG
CAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAAGGGCAGAAAAGTTTTTTATTCCA
GCG

>TRINITY_DN88635_c0_g1_i1 from sample A4 / Example unspliced isoform:

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGgtaagttcttcaatttgaattcttccctgtcctgtgcttgacagttaacaattgtatttgtggt
attaagcactattggctagaaaaatatatgaattctttccgtgcctccgattttgtcttctggtgtggattgacatttacaattacaaatattaagataggatttaagttgtatgtgtttttgcgcgctttgtgaagtttgtatattgcccaataacagACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTA
GGCCATCGCGGTTCTGGTAAGAACAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTTCCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAA
GGGATTGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGA
AAAGCCAATGATGGCAGTATTTGCAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAA
GGGCAGAAAAGTTTTTTATTCCAGCG

Intron 4 GTAAGTTCTT ... CGATGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	9	7	8
A2	36.42	12	10.8	3
A3	50.59	5	6.3	4
A4	37.93	9	8	3
A5	40	6	1	7
A6	47.83	6	3	8
A7	43.59	11	10	7
A8	46.67	8	5	9
A9	40.54	11	5	10
A10	53.85	3	2	5

>TRINITY_DN113169_c0_g1_i1 from sample A1 / Example spliced isoform

AAGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAG
CAGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGA
TTGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGAAAAG
CCAATGATGGCAGTATTTGCAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAAGGGC
AGAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAGAGCAATTTT
ACAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAAGTGAGTATTATTCCAATAATCTATCTATCTTGAATTACACATCCATAATACTAATAAGATAAGGTT

>TRINITY_DN113169_c0_g1_i5 from sample A1 / Example unspliced isoform:

AAGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAG
CAGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGgtaagttcttcaatttgaattcttccctgtcctgtgcttgacagttaacaattgtatttgtgg
tattaagcactattggctagaaaaatatatgaattctttccgtgcctccgattttgtcttctggtgtggattgacatttacaattacaaatattaagataggatttaagttgtatgtgtttttgcgcgctttgtgaagtttgtatattgcccaataacagaccataaaatagaagacgggcaacaatcgctgactgttct
aggccatcgcggttctggtaagaacagaatatttagtgctggagacacatctaattccagaccttccattatggaaaataccataaaatctttggccctggccgcacagaatggcgtagattttgttgaattcgatgtacagGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACA
AGGGATTGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCG
AAAAGCCAATGATGGCAGTATTTGCAAATGGATTGTAAATGTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAA
AGGGCAGAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAGAGCA
ATTTTACAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACATCCTC
GGATGACATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCATGCTACAATCTAGCAATGGCTTTTGCGTTGTCTTTGGCCCATCAATGTCTTTTTGCTTTGTTCTTACCCCTTTCCG
CATCATGGGGAACATGTGTTGTTGTTGGAATCCACGTGCCTGGATCCTCTTTAGTCTCGACTCTCTCATTTTACCTGATTGTGATACCGTGCGTTCCAACTTCCCCGCACCAATTTTCAAATAACAAAACAGGCG

Intron 5 GTAAGACACT ... TGTTGCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	12	5	7
A2	35.29	11	8	4
A3	17.65	14	6	0
A4	33.33	7	2	5
A5	40	6	5	3
A6	53.85	3	5	2
A7	0	1	0	0
A8	17.95	16	1	6
A9	11.11	12	2	1
A10	20	4	1	1

>TRINITY_DN105170_c0_g1_i5 from sample A2 / Example spliced isoform

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGAT
TGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGATCCAAAGCCCTCTGGTTCGAAAAGC
CAATGATGGCAGTATTTGCAAATGGATTGTAAATCTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTATGGAAAGGGCA
GAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAGAGCAATTTTA
CAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACATCCTCGGATGA
CATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCG

>TRINITY_DN105170_c0_g1_i6 from sample A2 / Example unspliced isoform:

GTGGATTGACATTTACAATTACAAATATTAAGATAGGATTTAAGTTGTATGTGTTTTTGCGCGCTTTGTGAAGTTTGTATATTGCCCAATAACAGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAACAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTT
CCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTAGACTAAGCTTGTCATCTTCTGGCATATGATATGCTGTTTAGGCTTGTTGCCTTCTTAGAACTTGGAGATTTGCTTTGTTTATAAAATGTTTAAGCTTGTTTTTGTCTTTAGCAGAAATTC
TAAGGTTGTTTTAAATTTGCTTCAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGTAATTTGCTTTCTATATTTTTGTTTCCTGTTTCATTCAAACTGTATTTTGTTCGAAAGTCTTTCAAGATGCAAAAAGGAATATAACTTTACCTAGGGATCAGAGTGTTGAAGGG
TTCCATTCTTATGTAGGTTTATCCATCTTGCAGGTTTAGGGACCTTGTGGGACTCGGCCAAATATCTTATATTTTGCAACTAGACCGTTCTAACTTGTCTTTTGTTATGATCCGTGTTTCAGGGATTGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGT
GATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGgtaagacactgtagaggttcgtggatgaaattcagtttctggtgatgtgcaatttgtgatcattgtttctttgttgttgcttagATCCAAAGCCCTCTGG
TTCGAAAAGCCAATGATGGCAGTATTTGCAAATGGATTGTAAATCTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTAT
GGAAAGGGCAGAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAG
AGCAATTTTACAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACAT
CCTCGGATGACATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCG

Intron 6 GTAATTTGCT ... CGTGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.45	5	0.3	3
A2	36.46	10	4.5	7
A3	31.41	9	1.3	7
A4	63	3.3	8	3
A5	12.64	3.8	0	1
A6	4.41	5.5	0.8	0
A7	17.37	3.5	0.5	1
A8	26.66	3.5	0.5	2
A9	20.85	9	0.8	4
A10	6.25	2.5	0.5	0

>TRINITY_DN105170_c0_g1_i3 from sample A2 / Example spliced isoform

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAA
CAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTTCCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGATTGCATAATGCGAGGCGCA
TTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAG

>TRINITY_DN105170_c0_g1_i6 from sample A2 / Example unspliced isoform:

GTGGATTGACATTTACAATTACAAATATTAAGATAGGATTTAAGTTGTATGTGTTTTTGCGCGCTTTGTGAAGTTTGTATATTGCCCAATAACAGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAACAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTT
CCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTAGACTAAGCTTGTCATCTTCTGGCATATGATATGCTGTTTAGGCTTGTTGCCTTCTTAGAACTTGGAGATTTGCTTTGTTTATAAAATGTTTAAGCTTGTTTTTGTCTTTAGCAGAAATTC
TAAGGTTGTTTTAAATTTGCTTCAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGgtaatttgctttctatatttttgtttcctgtttcattcaaactgtattttgttcgaaagtctttcaagatgcaaaaaggaatataactttacctagggatcagagtgttgaaggg
ttccattcttatgtaggtttatccatcttgcaggtttagggaccttgtgggactcggccaaatatcttatattttgcaactagaccgttctaacttgtcttttgttatgatccgtgtttcagGGATTGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGT
GATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGGTAAGACACTGTAGAGGTTCGTGGATGAAATTCAGTTTCTGGTGATGTGCAATTTGTGATCATTGTTTCTTTGTTGTTGCTTAGATCCAAAGCCCTCTGG
TTCGAAAAGCCAATGATGGCAGTATTTGCAAATGGATTGTAAATCTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTAT
GGAAAGGGCAGAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAG
AGCAATTTTACAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACAT
CCTCGGATGACATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCG

Intron 7 GTAGACTAAG ... TTTGCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.91	8	4	3
A2	47.73	3	3	2.5
A3	19.19	4	1	1
A4	39.39	3	3	1
A5	46.08	7	8	4
A6	8.49	8	1	0.5
A7	24.27	7	4	0.5
A8	24.87	9	5	1
A9	33.03	10	4	6
A10	39.34	5	3	3.5

>TRINITY_DN105170_c0_g1_i3 from sample A2 / Example spliced isoform

AGCAAACTCAAATATTTCGAGGAATTCGGGAGGAAAGGAGAAAGCGCGTGAAGTTGTCAGTACTTTCTTCAATTGATCGACTGCTGCCAGCTCTGCGGGCGGACTCGTGTGAGTACATATTCATTAGTTTTGTTCGGCAACGCCATCAATTGTCTTCGCCGGACTTGACATTTTCAGATTTGGACTGAGTAATCCGCAGC
AGTCCGGCAATGGCTCTTCATTCATCAATACTTGCACGGTCGATGAATATAGATCCCGCTGTAATGCAGCAGGCTTCCACCCCTGCAAATCTCAATATCCTATACACAGGCCCGCGAATGGCCCAATTTTTTCGTGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAA
CAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTTCCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGGATTGCATAATGCGAGGCGCA
TTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGTGATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAG

>TRINITY_DN105170_c0_g1_i6 from sample A2 / Example unspliced isoform:

GTGGATTGACATTTACAATTACAAATATTAAGATAGGATTTAAGTTGTATGTGTTTTTGCGCGCTTTGTGAAGTTTGTATATTGCCCAATAACAGACCATAAAATAGAAGACGGGCAACAATCGCTGACTGTTCTAGGCCATCGCGGTTCTGGTAAGAACAGAATATTTAGTGCTGGAGACACATCTAATTCCAGACCTT
CCATTATGGAAAATACCATAAAATCTTTGGCCCTGGCCGCACAGAATGGCGTAGATTTTGTTGAATTCGATGTACAGgtagactaagcttgtcatcttctggcatatgatatgctgtttaggcttgttgccttcttagaacttggagatttgctttgtttataaaatgtttaagcttgtttttgtctttagcagaaattc
taaggttgttttaaatttgcttcagGTCACTAAAGACGGCCATGCTGTCATCTTCCATGACGACTATATTATAATAGATGACAAGGTAATTTGCTTTCTATATTTTTGTTTCCTGTTTCATTCAAACTGTATTTTGTTCGAAAGTCTTTCAAGATGCAAAAAGGAATATAACTTTACCTAGGGATCAGAGTGTTGAAGGG
TTCCATTCTTATGTAGGTTTATCCATCTTGCAGGTTTAGGGACCTTGTGGGACTCGGCCAAATATCTTATATTTTGCAACTAGACCGTTCTAACTTGTCTTTTGTTATGATCCGTGTTTCAGGGATTGCATAATGCGAGGCGCATTGGACAACTGGGATTCAGCAGACTTTCTTTTCCACCAAACCGTTCTAACTTTCGT
GATGGTCTGTCTGTTTCAGGGATTACACAATGCAAGGCGCATTGGAGACCTCACTCTTGAAGAATATAATTCCATGGGATTTCAAGCCGATGGCCTGGAGGTAAGACACTGTAGAGGTTCGTGGATGAAATTCAGTTTCTGGTGATGTGCAATTTGTGATCATTGTTTCTTTGTTGTTGCTTAGATCCAAAGCCCTCTGG
TTCGAAAAGCCAATGATGGCAGTATTTGCAAATGGATTGTAAATCTGCAAGACCGCCTATGTACTCTTAAAGATGCGTGCGAGAAAGTTCCTCTCTCTGTGGGATTCAACATAGAGCTGAAATTTGATGATGAAATTGAGGTTTCTGAAGTAGATCTTAAATGGGTGATTGGTACCGTTCTCAAGGAAGTGGCGATGTAT
GGAAAGGGCAGAAAAGTTTTTTATTCCAGCTTCCATCCAGATGCAGTGGATTTATTGCGTAAATTGCAGACTGAGTACCCGGTACTCTTTTTAACAGATGGGGGTACTCATATTTACAAGGACGAGAGAAGAAATTCCATTGAAAGTGCGGTAGAAGTTTGTCTAAAATGTGGATTGCAAGGAATTGTCAGCGAAGTTAG
AGCAATTTTACAGAACCCTGGATTAGTCTCCAAAGTTAAGGATGCTGGATTATGCCTACTCACTTACGGGGAACTTAATAATTTTGCTGAAGCATTGCAGAAGCAGAAAGAAACGGGCGTTGATGGGGTGATAGTGGATCATGTTCTAGAGATGGTTACCGCAGTTCGAAATGCAACGATTTCTGATGGCACGTTGACAT
CCTCGGATGACATCCGCTCTAGGGATATATCTTCGATTGTCAAAGCAGCTGCGTAGAAGAGCACCTATAAGTTTTCATTAATGTTAAAAAAAAACACTCATTATCGTCATTGTATTATTTATTTGGCG