sp|Q9LMP7|GOS11_ARATH ----> Golgi SNAP receptor complex member 1-1

Intron 1 GTAGGTGAAC ... TTATAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.48	14.1	33.9	44.4
A2	63.02	24.4	41.6	42
A3	65.48	25	42	53
A4	77.56	8.9	32.7	29
A5	65.5	6.4	11.8	12
A6	61.65	240.9	349.4	425.8
A7	66.72	152.8	253.4	360.8
A8	70.41	191	359	553
A9	67.55	167.8	262.4	438.6
A10	59.59	122	178.2	184

>TRINITY_DN109116_c0_g1_i5 from sample A5 / Example spliced isoform

GCGGTGGTGTGGGTCTGGTTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTGTATATTTAAACACTTCCATTCGAGAGATTGATCACTACATATGGTTTACATTTCTTTCCGAATTCTGTCTGAGACGGCTCATTCATAATTTTCTCTCAATTGAATGTTTTCGATTTTTTGCTCTT
TCTTTTCTTTTCTGAATGTCCATTCATTTCTGTTATTAGTTGCTGTTGTTTAAACTCAATTTCTTCCTGTTTTCATTTATTTTCTTCTGTTAATTGCTGCTATTTAATCTTAATTTCTTGCTGCTTACAATTTTGTTTCTGAAATTCTACTTCTACCAGACTTTTCATGTTTTTCTGGGATGGGATGGGATAGGGTTTCT
GCATTGCTATTGTATTTATGCCTCATTTATTGATTTTTCCCAATATTTTAAAGCCTTGACACGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACG
ACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGCCCAAGCTACCTTTTGAA

ATATGTGTAGAGCTCATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTGAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATATTGGATTCATTACCCACGTTTTATTTTTGGATGTATAGTTTACCTTTATTGCTAGTATGATTATCTAAAAA
CTCCCCCAATCTTATGTATCTAAATTCTTTTTCAAGGAACAAAATTCTTCCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAAT
CTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTTTTTTTCTTGAGTGGCATTTTCTGGCCTGCAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTA
TCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAG
TACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAAAAAA

>TRINITY_DN109116_c0_g1_i11 from sample A5 / Example unspliced isoform:

ATTTCATTTTAGCTGGTAGCTCCTGTTGTATCTATGATTGTACTTCATATTTTGGTTTCATTAGCCTTGATTTCTTGGCGAACCAGTATCCATTACAGAATCATTCTTCATAATCTCTTCCAGGATTGGCTCACTTAACATTTTTATAAGAGATGACTGGGATTCAATACAGTTTTAAATGTTTCACTGTTCTCATGATG
TTTATCAAGTTAGCATATATTGTTCTTGGTTTTCCTGTTATTGTTCTTAGCATATATTTAATGCTGGTAGCTGTTTTCCATGGAATTTCTGTGGGCTGAATTTTCTACAAGATCTTTGCTGTTGTAGATGTTATCCAACATTACGATGTCTTAATGACCATTGTCAATCTTATCCAGATAGACAATGTGATACTCCAGGC
GAGTCCAGGCCCAAGCTACCTTTTGAAATATGTgtaggtgaaccatattctgtccgcaatttgtcgcagaaaatcaatggacacaataatactttctcttgtagcatctgcttgcactattttcattctgttatactggttttcaaagtaagctatatcttacaaattcggaaattatggtatgctttataagcagGTAG
AGCTCATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTGAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATATTGGATTCATTACCCACGTTTTATTTTTGG

Intron 2 GTATGAAAGG ... ATCATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	6	5	4
A2	37.93	9	7	4
A3	33.33	3	3	0
A4	41.18	5	5	2
A5	33.33	1	0	1
A6	39.31	44	43	14
A7	33.33	56	42	14
A8	39.24	24	18	13
A9	39.51	49	31	33
A10	36.36	28	25	7

>TRINITY_DN46097_c1_g1_i7 from sample A8 / Example spliced isoform

TTCATATATTTTTTAAATGTAGTTATTTGCGAGGATGCTGCTTCTTTAATGTAATTTTAAACCCTAGCTATTTGCCATTCAGATGGACAGTGGGAATAAATTGTTCCACGTCGCTTTCAACCATAAGAAAAACACGTCTTCCCTCAACTTTGCCGTTTAGGGAATGGTAGCAAGGACAAGCCGCCGTGGGGAGAACAAAT
AAATCTAAATGTGACTAGGTCGGTCGGAGGGATCATATAAGTTCAATTCACCGAATGGGAAGTACATTAAGAAGCAGGTCGTTCTTTCAATTCCCATTAGGGCTTTATGTTCAGTCGGGATAGGGTTTCTTCCCACTGTTCTTTATGCTACGGCGCAGAGTGCTTCGGGAGTTTCCTTGGCGTTCGAAAGCTTGGTCAGT
GACATAAATAACGAAGTAGGGAATTAAATCCCACTAAACAGATGAATGGGATTCATAATGGTGATAAGCAGCATTAGGGGAAAGTAAGATGGGACGAATTGGCTAAAGAAGGTATGAAAGGGATGAGTAAAATCATGGTTTGAGTAGAAGATTTCAGTGCAGTTGGTCTTAGTGTTTTGGGTGTGCACAAACCGAGAGCC
TTTTGGATCCTACATCTGAAATGTATGCATCTCTTTCATGAATTAAATCCCACTAAACAGATGAATGGGATTCATAATGGTGATAAGCAGCATTAGGGGAAAGTAAGATGGGACGAATTGGCTAAAGAAGGTATGAAAGGGATGAGTAAAATCATGGTTTGAGTAGAAGATTTCAGTGCAGTTGGTCTTAGTGTTTTGGG
TGTGCACAAACCGAGAGCCTTTTGGATCCTACATCTGAAATGTATGCATCTCTTTCATGTATGTATGCATCTTATCTCTGTAGTAGCTAGCTTCTCTTATTTTCAATAACTCTGAAACATAATGAAAATGGTTACATAGGTAGATGCTGGTGGAAAGGTGGAGTTTCCCTTCTCATTGTTTAGGGATCGAGAACATGTCT
CAAACAATAACTCAACTTTTTATATCCACTCGTATTTCTTTTATCTTTTAAAACTTATGTAACCATCACTTCTTTGAGCGAATGAATTTCATCGGTATCTTTAGCCACATTTACATTATCCCCATATTCATTTGTAACTTCGTGTGTGTTTATCAAATTTTCGACTACAATTGGATACTTAGTTGTAGTATCAACTGCTT
TTTGGATCTTCAACTGTATTTACCAAAGCTTCAATTGCATCTAAATCCTCCCC

>TRINITY_DN46097_c1_g1_i5 from sample A8 / Example unspliced isoform:

TTCATATATTTTTTAAATGTAGTTATTTGCGAGGATGCTGCTTCTTTAATGTAATTTTAAACCCTAGCTATTTGCCATTCAGATGGACAGTGGGAATAAATTGTTCCACGTCGCTTTCAACCATAAGAAAAACACGTCTTCCCTCAACTTTGCCGTTTAGGGAATGGTAGCAAGGACAAGCCGCCGTGGGGAGAACAAAT
AAATCTAAATGTGACTAGGTCGGTCGGAGGGATCATATAAGTTCAATTCACCGAATGGGAAGTACATTAAGAAGCAGGTCGTTCTTTCAATTCCCATTAGGGCTTTATGTTCAGTCGGGATAGGGTTTCTTCCCACTGTTCTTTATGCTACGGCGCAGAGTGCTTCGGGAGTTTCCTTGGCGTTCGAAAGCTTGGTCAGT
GACATAAATAACGAAGTAGGGAATTAAATCCCACTAAACAGATGAATGGGATTCATAATGGTGATAAGCAGCATTAGGGGAAAGTAAGATGGGACGAATTGGCTAAAGAAGgtatgaaagggatgattaaaatcatggtttgagtagaagatttcagtgcagttgttctttagcgtttaggctatgcacaaacctaacct
agagccttttggatccgacatttgaaatgtattcatttctttcatgtacatgtatgcatcttatctcttcactagctagcttctcttattttttgaactgagacctacatatcattgcagGTATGAAAGGGATGAGTAAAATCATGGTTTGAGTAGAAGATTTCAGTGCAGTTGGTCTTAGTGTTTTGGGTGTGCACAAA
CCGAGAGCCTTTTGGATCCTACATCTGAAATGTATGCATCTCTTTCATGAATTAAATCCCACTAAACAGATGAATGGGATTCATAATGGTGATAAGCAGCATTAGGGGAAAGTAAGATGGGACGAATTGGCTAAAGAAGGTATGAAAGGGATGAGTAAAATCATGGTTTGAGTAGAAGATTTCAGTGCAGTTGGTCTTAG
TGTTTTGGGTGTGCACAAACCGAGAGCCTTTTGGATCCTACATCTGAAATGTATGCATCTCTTTCATGTATGTATGCATCTTATCTCTGTAGTAGCTAGCTTCTCTTATTTTCAATAACTCTGAAACATAATGAAAATGGTTACATAGGTAGATGCTGGTGGAAAGGTGGAGTTTCCCTTCTCATTGTTTAGGGATCGAG
AACATGTCTCAAACAATAACTCAACTTTTTATATCCACTCGTATTTCTTTTATCTTTTAAAACTTATGTAACCATCACTTCTTTGAGCGAATGAATTTCATCGGTATCTTTAGCCACATTTACATTATCCCCATATTCATTTGTAACTTCGTGTGTGTTTATCAAATTTTCGACTACAATTGGATACTTAGTTGTAGTAT
CAACTGCTTTTTGGATCTTCAACTGTATTTACCAAAGCTTCAATTGCATCTAAATCCTCCCC

Intron 3 GTTTACATTT ... TATTTTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.92	33	14	110
A2	78.45	25	19	91
A3	76.67	21	29	69
A4	82.56	15	11	71
A5	77.08	11	14	37
A6	74.85	84	41	250
A7	72.32	80	49	209
A8	74.12	81	40	232
A9	76.4	76	47	246
A10	81.95	37	54	168

>TRINITY_DN45096_c0_g2_i4 from sample A6 / Example spliced isoform

CAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTGTATATTTAAACACTTCCATTCGAGAGATTGATCA
CTACATATGCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGAC

>TRINITY_DN45096_c0_g2_i3 from sample A6 / Example unspliced isoform:

CAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTGTATATTTAAACACTTCCATTCGAGAGATTGATCA
CTACATATGgtttacatttctttccgaattctgtctgagacggctcattcataattttctctcaatggaatgttttcgatttcatttttgaacagtttgctctttcttttcttttctgaatgtccattcatttctgttattagttgctgttgtttaaactcaatttcttcctgttttcatttattttcttctgttaattg
ctgctatttaatcttaatttcttgctgcttacaattttgtttctgaaattctacttctaccagacttttcatgtttttctgggatgggatgggatggggtttctgcattgctattgtatttatgcctcatttattgatttttcccaatattttaaagCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTAT
CCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTT
CGCAGGTTTCACTGCGGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATT
CTGTTATACTGGTTTTCAAAGTAAGCTATATCTTACAAATTCGGAAATTATGGTATGCTTTATAAGCAGGTAGAGCTCATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTTAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATATTGGATTC
ATTACCCACGTTTTAT

Intron 4 GTAGGCGAAT ... TATTTTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76	34.8	8	110.2
A2	75.11	30	11	90.5
A3	72.22	26.7	16	69.4
A4	78.03	20.1	9	71.4
A5	76.82	11.1	6	36.8
A6	65.7	129.9	22	248.8
A7	68.66	95.3	35.8	208.8
A8	69.79	100.5	18	232
A9	70.38	103	33	244.8
A10	70.71	69.6	23	168

>TRINITY_DN120501_c1_g1_i6 from sample A2 / Example spliced isoform

CGAAAATGACAGCAAACGCAAAACAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACT
GTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATG
TCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTA
TTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTACAAATTCGGAAATTATGGTATGCTTTATAAGCAGGTAGAGCTCATGCTAAGATTTCT

>TRINITY_DN120501_c1_g1_i1 from sample A2 / Example unspliced isoform:

CGAAAATGACAGCAAACGCAAAACAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGgtaggcgaatggctgtgccttttttttgtatatttaaac
acttccattcgagagattgatcactacatatggtttacatttctttccgaattctgtctgagacggctcattcataattttctctcaatggaatgttttcgatttcatttttgaacagtttgctctttcttttcttttctgaatgtccattcatttctgttattagttgctgttgtttaaactcaatttcttcctgtttt
catttattttcttctgttaattgctgctatttaatcttaatttcttgctgcttacaattttgtttctgaaattctacttctaccagacttttcatgtttttctgggatgggatgggatagggtttctgcattgctattgtatttatgcctcatttattgatttttcccaatattttaaagCCTTGACAGGTATATCTGCA
CCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTAC
TGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTT
GTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTACAAATTCGGAAATTATGGTATGCTTTATAAGCAGGTAGAGCTCATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTTAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTC
AATGGCTTTGAAATGGATATTGGATTCATTACCCACGTTTTATTTTTGGTTGTATAGACCTTTATTGCTAGTATGATTATCTAAAAACTCCCCCAATCTTATGTATCTAAATTGTTTTTCAGGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGC
CTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTA
GGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAA
ACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACG

Intron 5 GCTAGAAAAT ... CACAGGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.19	16	35	62
A2	83.33	10	62	38
A3	81.94	13	74	44
A4	78.85	11	38	44
A5	85.92	5	40	21
A6	76.05	80	267	241
A7	79.24	63	288	193
A8	73.2	67	163	203
A9	85.22	45	335	184
A10	79.05	53	230	170

>TRINITY_DN46097_c1_g1_i9 from sample A8 / Example spliced isoform

CTTGGGACGAACTTCGGAAACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACATTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTT
GTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTT

>TRINITY_DN46097_c1_g1_i8 from sample A8 / Example unspliced isoform:

AAGGAATGAAGGTTCGGGGCAGTAATTAAGAGTGGTTTTGAATATACAGTGTACAGTTTTATTTTGAGGGTAAACCCTACTCCATCGTTGTGTTGTCATCTATAATAGCATTTAAATTGGTGAGTCCACCTCAAGTACGTATAAGGAAGTATCTGACGTGGAATGTCAACCTGGCAATTTCTTTTCTCTGTTACATAGGC
TCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGAACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAAAATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGgctagaaaattagaagggcaatt
ggatgagcagttgacttcttacagaagactagtcaacaccaaagttgagggctctgagcaagagaaggaagctggaatagagagattgctacagcaactgcagcatgtcaatgcacaaatgcaagtttgggtttcatcaggaagctcagagatcctttctcatactttgactcggcaccgagaaatttttaatgacctta
gtcaggaattcaagaggcttcgttcaagcctaaaagccagacgagagcatgaaacactgctacaaagtttcacttcatttgacaatgcaaatgatgatgttgaaagaagcagtgactcagcagagcaagcacttttgaaggagcaagcaagactacaaagtagcacaggacagATAGACAATGTGATACTCCATGCCCAA
GCTACGCTTGGTGCACTAATGTTTCAACGTTCGACATTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTA
ACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTT

Intron 6 GTTTGCTCTT ... TATTTTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.39	34	22	110
A2	76.47	28	27	91
A3	74.19	24	35	69
A4	81.61	16	16	71
A5	74	13	20	37
A6	71.43	100	84	250
A7	68.08	98	66	209
A8	69.46	102	61	232
A9	70.09	105	84	246
A10	75.68	54	71	168

>TRINITY_DN45096_c0_g2_i9 from sample A6 / Example spliced isoform

CAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTTGTATATTTAAACACTTCCATTCGAGAGATTGATC
ACTACATATGGTTTACATTTCTTTCCGAATTCTGTCTGAGACGGCTCATTCATAATTTTCTCTCAATGGAATGTTTTCGATTTCATTTTTGAACACCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCG
TCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCGGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAG

>TRINITY_DN45096_c0_g2_i3 from sample A6 / Example unspliced isoform:

CAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTGTATATTTAAACACTTCCATTCGAGAGATTGATCA
CTACATATGGTTTACATTTCTTTCCGAATTCTGTCTGAGACGGCTCATTCATAATTTTCTCTCAATGGAATGTTTTCGATTTCATTTTTGAACAgtttgctctttcttttcttttctgaatgtccattcatttctgttattagttgctgttgtttaaactcaatttcttcctgttttcatttattttcttctgttaattg
ctgctatttaatcttaatttcttgctgcttacaattttgtttctgaaattctacttctaccagacttttcatgtttttctgggatgggatgggatggggtttctgcattgctattgtatttatgcctcatttattgatttttcccaatattttaaagCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTAT
CCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTT
CGCAGGTTTCACTGCGGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATT
CTGTTATACTGGTTTTCAAAGTAAGCTATATCTTACAAATTCGGAAATTATGGTATGCTTTATAAGCAGGTAGAGCTCATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTTAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATATTGGATTC
ATTACCCACGTTTTAT

Intron 7 GTAAGCTATA ... TGTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65.54	24.6	46.8	25
A2	73.33	17.6	48.4	16
A3	65.44	31	58.6	14
A4	66.82	19.8	39.8	17
A5	68.65	5.8	12.4	20
A6	76.09	135	427.2	106
A7	80.91	87.6	368.5	59
A8	79.95	138.4	548.5	111
A9	79.31	111.8	426	121
A10	77.4	57.8	196	62

>TRINITY_DN120501_c1_g1_i5 from sample A2 / Example spliced isoform

TTTGAATATACAGTGTACAGTTTTATTTTGAGGGTAAACCCTACTCCATCGTTGTGTTGTCATCTATAATAGCATTTAAATTGGTGAGTCCACCTCAAGTACGTATAAGGAAGTATCTGACGTGGAATGTCAACCTGGCAATTTCTTTTCTCTGTTACATAGGCTCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGA
ACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAAAATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGTTGACTTCTTACAGAAGACTAGTCAA
CACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTGGAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAGGAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTCAAGAGGCTTCGTTCAAGCCTAAAAG
CCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACTTCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAGGAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACA
TTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCT
GCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTT
TTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTT
TCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTG
TCAGACG

>TRINITY_DN120501_c1_g1_i8 from sample A2 / Example unspliced isoform:

TTTGAATATACAGTGTACAGTTTTATTTTGAGGGTAAACCCTACTCCATCGTTGTGTTGTCATCTATAATAGCATTTAAATTGGTGAGTCCACCTCAAGTACGTATAAGGAAGTATCTGACGTGGAATGTCAACCTGGCAATTTCTTTTCTCTGTTACATAGGCTCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGA
ACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAAAATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGTTGACTTCTTACAGAAGACTAGTCAA
CACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTGGAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAGGAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTCAAGAGGCTTCGTTCAAGCCTAAAAG
CCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACTTCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAGGAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACA
TTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAgtaagctatatcttgcaaattcagaaattatggtatgct
ttacatgcaggtagagctcatgctaagatttctgatgtgtggtgcctggttttgaatattctgttatattggttaatttgttatgtagagatctgtatgatggttgttcacattcaatggctttgaaatggatattggattcattacccacgttttatttttggttgtatagacctttattgctagtatgattatctaaa
aactcccccaatcttatgtatctaaattgtttttcagGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAA
ATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTT
CTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAG
AAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACG

Intron 8 GTAAGCTATA ... TGTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.22	25	49	25
A2	73.02	17	46	16
A3	64.04	32	57	14
A4	67.24	19	39	17
A5	73.68	5	14	20
A6	77.08	124	417	106
A7	82.27	78	362	59
A8	81.33	126	549	110
A9	80.61	102	424	121
A10	78.84	51	190	62

>TRINITY_DN120501_c1_g1_i5 from sample A2 / Example spliced isoform

TTTGAATATACAGTGTACAGTTTTATTTTGAGGGTAAACCCTACTCCATCGTTGTGTTGTCATCTATAATAGCATTTAAATTGGTGAGTCCACCTCAAGTACGTATAAGGAAGTATCTGACGTGGAATGTCAACCTGGCAATTTCTTTTCTCTGTTACATAGGCTCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGA
ACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAAAATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGTTGACTTCTTACAGAAGACTAGTCAA
CACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTGGAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAGGAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTCAAGAGGCTTCGTTCAAGCCTAAAAG
CCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACTTCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAGGAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACA
TTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCT
GCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTT
TTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTT
TCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTG
TCAGACG

>TRINITY_DN120501_c1_g1_i7 from sample A2 / Example unspliced isoform:

CGAAAATGACAGCAAACGCAAAACAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTGTATATTTAAAC
ACTTCCATTCGAGAGATTGATCACTACATATGGTTTACATTTCTTTCCGAATTCTGTCTGAGACGGCTCATTCATAATTTTCTCTCAATTGAATGTTTTCGATTTTTTGCTCTTTCTTTTCTTTTCTGAATGTCCATTCATTTCTGTTATTAGTTGCTGTTGTTTAAACTCAATTTCTTCCTGTTTTCATTTATTTTCTT
CTGTTAATTGCTGCTATTTAATCTTAATTTCTTGCTGCTTACAATTTTGTTTCTGAAATTCTACTTCTACCAGACTTTTCATGTTTTTCTGGGATGGGATGGGATAGGGTTTCTGCATTGCTATTGTATTTATGCCTCATTTATTGATTTTTCCCAATATTTTAAAGCCTTGACAGGTATATCTGCACCTGCGGCCCGCG
GGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTG
TTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTT
GCACTATTTTCATTTTGTTATACTGGTTTTCAAAgtaagctatatcttacaaattcggaaattatggtatgctttataagcaggtagagctcatgctaagatttcttatgtgtggtgcctggttttaatattctgttaaattggttaatttgttatgtagagatctgtatgatggttgttcacattcaatggctttgaaa
tggatattggattcattacccacgttttatttttggttgtatagacctttattgctagtatgattatctaaaaactcccccaatcttatgtatctaaattgtttttcagGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGT
AATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATT
TCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGC
TCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACG

Intron 9 GTAGGCAGTT ... TTTGAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.37	43	60	71
A2	74.15	19	55	54
A3	66.99	17	34	35
A4	79.26	14	64	43
A5	60.87	9	19	9
A6	11.85	160	39	4
A7	14.38	131	37	7
A8	14.19	133	39	5
A9	13.99	126	38	3
A10	16.58	83	23	10

>TRINITY_DN44881_c2_g1_i2 from sample A9 / Example spliced isoform

GAATAAACAGACGGAAAATAACATGCAACGAGTGGGAGTGGAGACAGATTAAAACGATGAATAAACAGACGGAAAATAACATGCAACGAGTGGGAGTGGAGACAGATTAAAACGATGAATAAACAGACGGAAAATAACAAACACACTCACTTCACTGACGGCGAAAATGACAGGAAACGCAAAGCAGTTCGCCTCTGCAT
TAACGCCGTTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGTAGCAAAATCAAGAAGAGAGGTGGTGTGGGTCTGGTTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGTCTTTTTTGTAAATTTAAACACTTCCATTCGAGAGATTGATCGCTACATATGGTTTACATTTCTTTC
CGAATTCTGTCTGAGACGGCTCATTCATAATTTTCTCTCAATTGAATGTTTTCGATTTCATTTTTGAACAGTTTGCTCTTTCTTTTCTTTTCTGAATGTGCATTCATTCCTGTTATTAGTTGCTGTTGTTTAAACTCAATTTCTTCCTGTTTTCATTTATTTTCTTCTGTTAATTGCTGCTATTTAATCTTAATTTCTTG
CTGCTTACAATTTTGTTTCTGAAATTCTACTTCTACTAGACTTTTCATGTTTTTCTGGGATGGGATGGGATAGGGTTTCTGCATTGCTATTGTATTTATGCCTCATTTATTGATTTTTCCCAATATTTTAAAGCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAG
CTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTGATTCTGTCTGTCCAAGCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACATGGAGGATAATGGATGACATCGCTCGGTTAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGA
TGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGGCAAGTACGAGTCAACCCCTTTCCCAGTGGGATTTATACTTTTTTTTTCAACCAACCAACAAACTTACCATTTTGACCACCTCTTTTGCGGGATGCCATTCAAATGAAAGACTGAAGGGAGGTGGCATAGTTGTTTTCAG
AACTCATATGGCAGAGGGCCATCCATAATTTAACTAGACATGTTTTTCGATATTTAAACATAGGTTTGTATTTTGTGGATAGTATTTATTGTAAACTCATTGGTGCTTATTACAATGCCTGGCTTAGTAAGGATATCGATAGAGGCATGCAACTGAGTCAGCTAGTTCATGAGAGTGATTCTAAGACCTTTCGTGCATTG
AGCTCAAAGCACTCAACCATTCAAGCTATCCTACTGAACTATTTATGAAGTTCTTTGTTATTTATGAGGTATATCATTGTTTTCTATGCATTTGTGTTTCTGATGTTAGATCTAGGTTTAGCCTAGGTGTTTTCAAAAACTCGTGTGGCAGAAGGTCATCCATATTTGAACTAACCAAGTTCTTTGATTTATCCCTAGAA
TTTAACCAAACATGTTTTTCAGTATTTAAACATAGGTTTGTATTTTGTGGATGTTACCTATTGTAAACTCATTGGTGCCGATTACAATGCCTGACCTAGTAAGGATATTGAGTGAGGCATGCAACAGAGTCAACTAGTCTGTGAGATTGATTCTGAGACCTCTTGCCAAAGCACTCAACCATTCAAGCTATCCTACTGAA
CTATTTATGAAGTTCTTTGTTATTTATGAGGTATATCATTGTTTTCTATGCATTTGTGTTTCTGATGTTAGATCTAGGTTTAGCCTAGGTGTTTTCAAAAACTCGTGTGGCAGAAGGTCATCCATATT

>TRINITY_DN44881_c2_g1_i4 from sample A9 / Example unspliced isoform:

TAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGgtaggcagtttgccttttcgttcttacgagtatataaactccattttatctccgtcttgttttgtttgaaaatgaaatatggtgaaaaaaggttttactga
atacttcctctaggtagaaatgatataaattatatgtaggtctaattgaaatgatttatagatacctttttgaaccgggcaaacactatcacatacttttcaatgggatttttaccctcctcacttggcaagcactattattcgccttgccgttctatcaggcaggaaatcacttacatcgtttgaatcagGCAAGTACG
AGTCAACCCCTTTCCCAGTGGGATTTATACTTTTTTTTTCAACCAACCAACAAACTTACCATTTTGACCACCTCTTTTGCGGGATGCCATTCAAATGAAAGACTGAAGGGAGGTGGCATAGTTGTTTTCAGAACTCATATGGCAGAGGGCCATCCATAATTTAACTAGACATGTTTTTCGATATTTAAACATAGGTTTGT
ATTTTGTGGATAGTATTTATTGTAAACTCATTGGTGCTTATTACAATGCCTGGCTTAGTAAGGATATCGATAGAGGCATGCAACTGAGTCAGCTAGTTCATGAGAGTGATTCTAAGACCTTTCGTGCATTGAGCTCAAAGCACTCAACCATTCAAGCTATCCTACTGAACTATTTATGAAGTTCTTTGTTATTTATGAGG
TATATCATTGTTTTCTATGCATTTGTGTTTCTGATGTTAGATCTAGGTTTAGCCTAGGTGTTTTCAAAAACTCGTGTGGCAGAAGGTCATCCATATTTGAACTAACCAAGTTCTTTGATTTATCCCTAGAATTTAACCAAACATGTTTTTCAGTATTTAAACATAGGTTTGTATTTTGTGGATGTTACCTATTGTAAACT
CATTGGTGCCGATTACAATGCCTGACCTAGTAAGGATATTGAGTGAGGCATGCAACAGAGTCAACTAGTCTGTGAGATTGATTCTGAGACCTCTTGCCAAAGCACTCAACCATTCAAGCTATCCTACTGAACTATTTATGAAGTTCTTTGTTATTTATGAGGTATATCATTGTTTTCTATGCATTTGTGTTTCTGATGTT
AGATCTAGGTTTAGCCTAGGTGTTTTCAAAAACTCGTGTGGCAGAAGGTCATCCATATT

Intron 10 GTAGAGCTCA ... TGTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	23	46	25
A2	80.77	10	42	16
A3	69.74	23	53	14
A4	70.73	12	29	17
A5	70.59	5	12	20
A6	75.57	139	430	106
A7	74.64	122	359	59
A8	75.56	180	556.5	111
A9	75.69	141	439	121
A10	74.4	64	186	62

>TRINITY_DN118151_c0_g1_i15 from sample A3 / Example spliced isoform

GCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTGCAAATTCAGAAATTATGGTATGCTTTACATGCAGGTAACAAAATTCT
TGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGG
CTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGAC
TGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTA
TACAAAGATGGGCGTCCTAGTAATTGTCAGACGATAATTATTCAGACCATTGTTAGCTGCTGATTACATGCCTTATCTTTTAAGTTTTTACTTCCTAAACTCAACAGAGATCATAGTTAAGGAAGTAGAAATGGTATACGAAAACCATAAGAAACCTTTTATGTTAGTGTTAATGATTCTCTTTATTATTTCTAATTACA
GCGAGTGCCATGATTTTCTTTATTTTTATGGGATACAAAGACGGACATCCTACTAATTGTCTGACAAGAATAATTAAGATCATTGTCAGCTGCTGATTACATGCCTTATCTTTTAACTTCCTAATCTCGTCTTTTAACTTCCTAAACTCAACAGAGATCACAGTTAACAAAGTAGAAATAGTATACAAAAATCATAAGAA
ACATTTTATGTCAGTGTTAATGTTTATTTGTTTTGTGTGGCTGAGTGGAGGCCTACATGGTCTACTTAAGTGTTCTGTCCACCAATTATAGCCCCATGACATGTATATATATTGGCTGATACCAACCACTATATGTATCAA

>TRINITY_DN118151_c0_g1_i11 from sample A3 / Example unspliced isoform:

CCCTACTCCATCGTTGTGTTGTCATCTATAATAGCATTTAAATTGGTGAGTCCACCTCAAGTACGTATAAGGAAGTATCTGACGTGGAATGTCAACCTGGCAATTTCTTTTCTCTGTTACATAGGCTCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGAACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAA
AATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGTTGACTTCTTACAGAAGACTAGTCAACACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTG
GAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAGGAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTCAAGAGGCTTCGTTCAAGCCTAAAAGCCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACT
TCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAGGAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACATTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAG
TCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTGCAAATTCAGAAATTATGGTATGCTTTACATGCAGgtagagctcatgctaagatttctgatgt
gtggtgcctggttttgaatattctgttatattggttaatttgttatgtagagatctgtatgatggttgttcacattcaatggctttgaaatggatattggattcattacccacgttttatttttggttgtatagacctttattgctagtatgattatctaaaaactcccccaatcttatgtatctaaattgtttttcagG
TAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTA
AGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAAT
TTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTT
ATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACGATAATTATTCAGACCATTGTTAGCTGCTGATTACATGCCTTATCTTTTAAGTTTTTACTTCCTAAACTCAACAGAGATCATAGTTAAGGAAGTAGAAATGGTATACGAAAACCATAAGAAACCTTTTATGTTAGTGTTAATGATTCTCTTTATTA
TTTCTAATTACAGCGAGTGCCATGATTTTCTTTATTTTTATGGGATACAAAGACGGACATCCTACTAATTGTCTGACAAGAATAATTAAGATCATTGTCAGCTGCTGATTACATGCCTTATCTTTTAACTTCCTAATCTCGTCTTTTAACTTCCTAAACTCAACAGAGATCACAGTTAACAAAGTAGAAATAGTATACAA
AAATCATAAGAAACATTTTATGTCAGTGTTAATGTTTATTTGTTTTGTGTGGCTGAGTGGAGGCCTACATGGTCTACTTAAGTGTTCTGTCCACCAATTATAGCCCCATGACATGTATATATATTGGCTGATACCAACCACTATATGTATCAA

Intron 11 GTAAGCTATA ... TTACATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.69	25	35	23
A2	48.66	17	22.3	10
A3	52.41	32	47.5	23
A4	47.03	19	21.8	12
A5	53.41	5	6.5	5
A6	61.05	124	249.8	139
A7	68.2	78	212.5	122
A8	66.08	126	311	180
A9	64.32	102	226.8	141
A10	66.31	51	136.8	64

>TRINITY_DN118151_c0_g1_i6 from sample A3 / Example spliced isoform

CCCTACTCCATCGTTGTGTTGTCATCTATAATAGCATTTAAATTGGTGAGTCCACCTCAAGTACGTATAAGGAAGTATCTGACGTGGAATGTCAACCTGGCAATTTCTTTTCTCTGTTACATAGGCTCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGAACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAA
AATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGTTGACTTCTTACAGAAGACTAGTCAACACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTG
GAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAGGAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTCAAGAGGCTTCGTTCAAGCCTAAAAGCCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACT
TCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAGGAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACATTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAG
TCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATG
CCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATT
AGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATA
AACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACGATAATTATTCAGACCATTGTTAGCTGCTGAT
TACATGCCTTATCTTTTAAGTTTTTACTTCCTAAACTCAACAGAGATCATAGTTAAGGAAGTAGAAATGGTATACGAAAACCATAAGAAACCTTTTATGTTAGTGTTAATGATTCTCTTTATTATTTCTAATTACAGCGAGTGCCATGATTTTCTTTATTTTTATGGGATACAAAGACGGACATCCTACTAATTGTCTGA
CAAGAATAATTAAGATCATTGTCAGCTGCTGATTACATGCCTTATCTTTTAACTTCCTAATCTCGTCTTTTAACTTCCTAAACTCAACAGAGATCACAGTTAACAAAGTAGAAATAGTATACAAAAATCATAAGAAACATTTTATGTCAGTGTTAATGTTTATTTGTTTTGTGTGGCTGAGTGGAGGCCTACATGGTCTA
CTTAAGTGTTCTGTCCACCAATTATAGCCCCATGACATGTATATATATTGGCTGATACCAACCACTATATGTATCAA

>TRINITY_DN118151_c0_g1_i15 from sample A3 / Example unspliced isoform:

GCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAgtaagctatatcttgcaaattcagaaattatggtatgctttacatgcagGTAACAAAATTCT
TGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGG
CTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGAC
TGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTA
TACAAAGATGGGCGTCCTAGTAATTGTCAGACGATAATTATTCAGACCATTGTTAGCTGCTGATTACATGCCTTATCTTTTAAGTTTTTACTTCCTAAACTCAACAGAGATCATAGTTAAGGAAGTAGAAATGGTATACGAAAACCATAAGAAACCTTTTATGTTAGTGTTAATGATTCTCTTTATTATTTCTAATTACA
GCGAGTGCCATGATTTTCTTTATTTTTATGGGATACAAAGACGGACATCCTACTAATTGTCTGACAAGAATAATTAAGATCATTGTCAGCTGCTGATTACATGCCTTATCTTTTAACTTCCTAATCTCGTCTTTTAACTTCCTAAACTCAACAGAGATCACAGTTAACAAAGTAGAAATAGTATACAAAAATCATAAGAA
ACATTTTATGTCAGTGTTAATGTTTATTTGTTTTGTGTGGCTGAGTGGAGGCCTACATGGTCTACTTAAGTGTTCTGTCCACCAATTATAGCCCCATGACATGTATATATATTGGCTGATACCAACCACTATATGTATCAA

Intron 12 GTAGGCGAAT ... TATTTTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.86	35	8	110
A2	75.21	30	11	91
A3	71.88	27	16	69
A4	77.53	20	9	69
A5	77.08	11	6	37
A6	65.71	131	22	251
A7	68.52	96	36	209
A8	69.55	102	18	233
A9	70.49	103	33	246
A10	70.59	70	23	168

>TRINITY_DN46097_c1_g1_i2 from sample A8 / Example spliced isoform

GAATAAACAGACGGAAAATAACATGCAACGAATGACAGTGGAGACAGATTAAAACGATGAATAAACAGACGGAAAATAACATGCAACGAATGACAGTGGAGACAGATTAAAACGATGAATAAACAGACGGAAAATAACAAACACACTCACTTCACTGACGGCGAAAATGACAGGAAACGCAAAGCAGTTCGCCTCTGCAT
TAACGCCGTTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGG
CGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCGGCAGCTACGAGTCTCCCTGGACCAT
GGCACTGAAAAAG

>TRINITY_DN46097_c1_g1_i1 from sample A8 / Example unspliced isoform:

GAATAAACAGACGGAAAATAACATGCAACGAATGACAGTGGAGACAGATTAAAACGATGAATAAACAGACGGAAAATAACATGCAACGAATGACAGTGGAGACAGATTAAAACGATGAATAAACAGACGGAAAATAACAAACACACTCACTTCACTGACGGCGAAAATGACAGGAAACGCAAAGCAGTTCGCCTCTGCAT
TAACGCCGTTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGgtaggcgaatggctgtgcctttttttttgtatatttaaacacttccattcgagagattgatcactacatatggtttacatttct
ttccgaattctgtctgagacggctcattcataattttctctcaattgaatgttttcgattttttgctctttcttttcttttctgaatgtccattcatttctgttattagttgctgttgtttaaactcaatttcttcctgttttcatttattttcttctgttaattgctgctatttaatcttaatttcttgctgcttacaa
ttttgtttctgaaattctacttctaccagacttttcatgtttttctgggatgggatgggatggggtttctgcattgctattgtatttatgcctcatttattgatttttcccaatattttaaagCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTC
AGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCGGCAGCTACGAGTCTCCCT
GGACCATGGCACTGAAAAAGGCAAGTACGAGTCAACCCCTTTCCCAGTGGGATTTATACTTTTTTTTTCAACCAACCAACAAACTTACCATTTTGACCACCTCTTTTGCGGGATGCCATTCAAATGAAAGACTGAAGGGAGGTGGCATAGTTGTTTTCAGAACTCATATGGCAGAGGGCCATCCATAATTTAACTAGACA
TGTTTTTCGATATTTAAACATAGGTTTGTATTTTGTGGATAGTATTTATTGTAAACTCATTGGTGCTTATTACAATGCCTGGCTTAGTAAGGATATCGATAGAGGCATGCAACTGAGTCAGCTAGTTCATGAGAGTGATTCTAAGACCTTTCGTGCATTGAGCTCAAAGCACTCAACCATTCAAGCTATCCTACTGAACT
ATTTATGAAGTTCTTTGTTATTTATGAGGTATATCATTGTTTTCTATGCATTTGTGTTTCTGATGTTAGATCTAGGTTTAGCCTAGGTGTTTTCAAAAACTCGTGTGGCAGAAGGTCATCCATATTTGAACTAACCAAGTTCTTTGATTTATCCCTAGAATTTAACCAAACATGTTTTTCAGTATTTAAACATAGGTTTG
TATTTTGTGGATGTTACCTATTGTAAACTCATTGGTGCCGATTACAATGCCTGACCTAGTAAGGATATTGAGTGAGGCATGCAACAGAGTCAACTAGTCTGTGAGATTGATTCTGAGACCTCTTGCCAAAGCACTCAACCATTCAAGCT

Intron 13 GTAGGCGAAT ... GCCTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.48	37	8	108
A2	76.56	30	10	98
A3	74.16	23	14	66
A4	77.53	20	8	69
A5	77.55	11	6	38
A6	65.36	133	21	251
A7	68.6	103	30	225
A8	68.68	114	18	250
A9	70.92	107	32	261
A10	68.4	85	23	184

>TRINITY_DN109116_c0_g1_i4 from sample A5 / Example spliced isoform

GCGGTGGTGTGGGTCTGGTTTTTTGCTTTTTTGTATATAGCCGTTGGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTT
TGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGCCCAAGCTACCTTTTGAAATATGTGTAGAGCTC
ATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTGAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATATTGGATTCATTACCCACGTTTTATTTTTGG

>TRINITY_DN109116_c0_g1_i9 from sample A5 / Example unspliced isoform:

GCGGTGGTGTGGGTCTGGTTTTTTGCTTTTTTGTATATAGCCGTTGGgtaggcgaatggctgtgccttttttttgtatatttaaacacttccattcgagagattgatcactacatatggtttacatttctttccgaattctgtctgagacggctcattcataattttctctcaatggaatgttttcgatttcatttttga
acagtttgctctttcttttcttttctgaatgtccattcatttctgttattagttgctgttgtttaaactcaatttcttcctgttttcatttattttcttctgttaattgctgctatttaatcttaatttcttgctgcttacaattttgtttctgaaattctacttctaccagacttttcatgtttttctgggatgggatg
ggatagggtttctgcattgctattgtatttatgcctcatttattgatttttcccaatattttaaagccttgacagGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAA
TGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGGTAGGCAGTTTGCCTTTTCGTTCTTACAAGTATATAA
ACTCCATTTAATCTCCGTCTTGTTTTGTTTGAAAATGAAATACGGTGAAAAAAGGTTTTACTGAATACGTCCTCTAGGTAGAAATGATATAAATTAGATGTAGGTCTAATTGAAATGATTTATAGATACCTTTTTGAACCCGGCAAACACTATCACATACTTTTCAATGGGATTTATACCCTCCTCACTTGGCAAGCACT
ATTATTCGCCTTGCCGTTCTATCAGGCAGGAAATCACTTACACCGTTTGAATCAGGCAAGTACGAGTCACCCCCTACGAGTCAACCCCTTTCCCAGTGGGATTTATACTTTTATTTTTCAATCAACCAACCAACTTACC

Intron 14 GTAAACCCTA ... TGTTACATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	4	0	14
A2	71.11	13	4	32
A3	67.92	17	0	36
A4	62.5	12	0	20
A5	70	3	0	7
A6	68.63	32	4	70
A7	70.1	29	5	68
A8	58.75	33	4	47
A9	69.57	42	14	96
A10	71.43	26	2	65

>TRINITY_DN48498_c1_g1_i1 from sample A10 / Example spliced isoform

GTTCGGGGCAGTAATTAAGAGTGGTTTTGAATATACAGTGTACAGTTTTATTTTGAGGGCTCAATTGCAGCCATGGAGGGGAGCCAAGACTCCTGAACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAAAATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAAT
GGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGTTGACTTCTTACAGAAGACTAGTCAACACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTGGAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAG
GAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTCAAGAGGCTTCGTTCAAGCCTAAAAGCCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACTTCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAG
GAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGGTGCACTAATGTTTCAACGTTCGACATTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAAT
ACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTGCAAATTCAGAAATTATGGTATGCTTTACATGCAGGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAAT
CTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCA
TACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCT
TATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACGATAATTATTCAGACCATTGTTAGCTGCTGC

>TRINITY_DN48498_c1_g1_i4 from sample A10 / Example unspliced isoform:

GTTCGGGGCAGTAATTAAGAGTGGTTTTGAATATACAGTGTACAGTTTTATTTTGAGGgtaaaccctactccatcgttgtgttgtcatctataatagcatttaaattggtgagtccacctcaagtacgtataaggaagtatctgacgtggaatgtcaacctggcaatttcttttctctgttacatagGCTCAATTGCAGC
CATGGAGGGGAGCCAAGACTCCTGAACGCCACTGTTTATCGGATCCGTTAAATTAGCATTGAAAATTGAAAAGGGCTGACAAGATTAGCGCGACAGCAAGAGAAAATCTGGGCAAATTACTCATAGAATGGATTTACCAGGGACTTGGGACGAACTTCGGAAACAGGCTAGAAAATTAGAAGGGCAATTGGATGAGCAGT
TGACTTCTTACAGAAGACTAGTCAACACCAAAGTTGAGGGCTCTGAGCAAGAGAAGGAAGCTGGAATAGAGAGATTGCTACAGCAACTGCAGCATGTCAATGCACAAATGCAAGTTTGGGTTTCATCAGGAAGCTCAGAGATCCTTTCTCATACTTTGACTCGGCACCGAGAAATTTTTAATGACCTTAGTCAGGAATTC
AAGAGGCTTCGTTCAAGCCTAAAAGCCAGACGAGAGCATGAAACACTGCTACAAAGTTTCACTTCATTTGACAATGCAAATGATGATGTTGAAAGAAGCAGTGACTCAGCAGAGCAAGCACTTTTGAAGGAGCAAGCAAGACTACAAAGTAGTACAGGACAGATAGACAATGTGATACTCCATGCCCAAGCTACGCTTGG
TGCACTAATGTTTCAACGTTCGACATTTGGAAATATTGGCTCAAAGATCAGCAATGTTAGCAGTCGGCTACCTTCAGTGAACCATATTCTGTCAGCAGTTCGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTT
GCAAATTCAGAAATTATGGTATGCTTTACATGCAGGTAGAGCTCATGCTAAGATTTCTGATGTGTGGTGCCTGGTTTTGAATATTCTGTTATATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATAATGGATTCATTACCCACGTTTTATTTTTGGTTGTATAGACC
TTTATTGCTAGTATGATTATCTAAAAACTCCCCCAATCTTATGTATCTAAATTGTTTTTCAGGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGTAATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAA
TAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATTTCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCA
TAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGCTCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATG
CAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACGATAATTATTCAGACCATTGTTAGCTGCTGC

Intron 15 GTAGGCGAAT ... TATTTTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.21	34.6	8	110.8
A2	74.75	30	11	88.8
A3	72.79	26.4	16	70.6
A4	78.48	19.8	9	72.2
A5	76.38	11.2	6	36.2
A6	65.45	128.4	21.6	243.2
A7	68.76	94.6	35.6	208.2
A8	70.01	99	18	230.8
A9	70.07	103	33	241.2
A10	70.83	69.2	23	168

>TRINITY_DN120501_c1_g1_i6 from sample A2 / Example spliced isoform

CGAAAATGACAGCAAACGCAAAACAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACT
GTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATG
TCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTTGCACTA
TTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTACAAATTCGGAAATTATGGTATGCTTTATAAGCAGGTAGAGCTCATGCTAAGATTTCT

>TRINITY_DN120501_c1_g1_i7 from sample A2 / Example unspliced isoform:

CGAAAATGACAGCAAACGCAAAACAGTTCGCCTCTGCATTAATGCCGGCGCCGCTAACGCCAGGAACCTCTAGTAGCTACACTGTCTCTATCTGTTGCAACAAAATCAAGAAGGGAGGTGGTGTGGGGCTGGCTTTTTGCTTTTTTGTATATAGCCGTTGGgtaggcgaatggctgtgccttttttttgtatatttaaac
acttccattcgagagattgatcactacatatggtttacatttctttccgaattctgtctgagacggctcattcataattttctctcaattgaatgttttcgattttttgctctttcttttcttttctgaatgtccattcatttctgttattagttgctgttgtttaaactcaatttcttcctgttttcatttattttctt
ctgttaattgctgctatttaatcttaatttcttgctgcttacaattttgtttctgaaattctacttctaccagacttttcatgtttttctgggatgggatgggatagggtttctgcattgctattgtatttatgcctcatttattgatttttcccaatattttaaagCCTTGACAGGTATATCTGCACCTGCGGCCCGCG
GGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCATCGCTCAGCCAGTCTTCAGTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTG
TTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGGCCAAGCTACCTTTGGAAATATGTGTAGGTGAACCATATTCTGTCCGCAATTTGTCGCAGAAAATCAATGGACACAATAATACTTTCTCTTGTAGCATCTGCTT
GCACTATTTTCATTTTGTTATACTGGTTTTCAAAGTAAGCTATATCTTACAAATTCGGAAATTATGGTATGCTTTATAAGCAGGTAGAGCTCATGCTAAGATTTCTTATGTGTGGTGCCTGGTTTTAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAA
TGGATATTGGATTCATTACCCACGTTTTATTTTTGGTTGTATAGACCTTTATTGCTAGTATGATTATCTAAAAACTCCCCCAATCTTATGTATCTAAATTGTTTTTCAGGTAACAAAATTCTTGCCTTGTGACATCTTAAATTTAGCTGCTCTTCTCTTGCTTGTTTTGCTTCTTACTCTAGCATGCCTTTTAAGCTGGT
AATCTGGGATTGATTATATATTGATAGGACGAATCTTCAGTCTGTAATAACTACATTTTGTTCATGAATGAAATCTTTACTGCCAAAAGTCCATCTGAAGCATTGTTTTAAGATTCAGAAGGCTGAATTGTATACTAGAGGACTGTTTTTTCTTGAGAGGCCTTTTCTGGCCTACAAGTTATGATTAGGCTGAAGAGATT
TCATACAAGGAACTAGGTCCACATAAGTCCCAGTCCTAAAATCTTCATAACTGGACCTGTTTAAAATTATTTCTATCTCACTTGCTATCTGACTACCATGGATCAACAATTTTCCTGATGACTGTTATGGTAGCCAGTATGGACTGTTTCTGAGAAAATTTGTCACTGCAACCACAAGGCATGATAAACTTGTTTATTGC
TCTTATTGCATTATGAACGAATTAAAGATAAATCATTGGGAGTTATGCAGTACAATCAATGCATGATTGCAGAAGTACTACTAATTACAGTGAGCGCCATGATTCTCTTTATTTTCATTGTATACAAAGATGGGCGTCCTAGTAATTGTCAGACG

Intron 16 GTAAACAAGT ... TTCTTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.38	100	0	32
A2	18.63	83.5	1	19
A3	11.43	77.5	0	10
A4	23.25	59.5	0	18
A5	19.73	69.5	9	17
A6	23.81	336	0	105
A7	23.09	323.5	0	97
A8	20.11	306.5	0	77
A9	22.5	335.5	0	97
A10	24.76	228	4	75

>TRINITY_DN109116_c0_g1_i9 from sample A5 / Example spliced isoform

GCGGTGGTGTGGGTCTGGTTTTTTGCTTTTTTGTATATAGCCGTTGGGTAGGCGAATGGCTGTGCCTTTTTTTTGTATATTTAAACACTTCCATTCGAGAGATTGATCACTACATATGGTTTACATTTCTTTCCGAATTCTGTCTGAGACGGCTCATTCATAATTTTCTCTCAATGGAATGTTTTCGATTTCATTTTTGA
ACAGTTTGCTCTTTCTTTTCTTTTCTGAATGTCCATTCATTTCTGTTATTAGTTGCTGTTGTTTAAACTCAATTTCTTCCTGTTTTCATTTATTTTCTTCTGTTAATTGCTGCTATTTAATCTTAATTTCTTGCTGCTTACAATTTTGTTTCTGAAATTCTACTTCTACCAGACTTTTCATGTTTTTCTGGGATGGGATG
GGATAGGGTTTCTGCATTGCTATTGTATTTATGCCTCATTTATTGATTTTTCCCAATATTTTAAAGCCTTGACAGGTATATCTGCACCTGCGGCCCGCGGGTACTGTATCCCATTAAAGATCCGAGAACCGAGCTACCGATTCAGCAGGGCGTCGCTCAGCCAGCCTTCCGTCCCTGCGACCATGGATGACGTATAACAA
TGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGGATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGGTAGGCAGTTTGCCTTTTCGTTCTTACAAGTATATAA
ACTCCATTTAATCTCCGTCTTGTTTTGTTTGAAAATGAAATACGGTGAAAAAAGGTTTTACTGAATACGTCCTCTAGGTAGAAATGATATAAATTAGATGTAGGTCTAATTGAAATGATTTATAGATACCTTTTTGAACCCGGCAAACACTATCACATACTTTTCAATGGGATTTATACCCTCCTCACTTGGCAAGCACT
ATTATTCGCCTTGCCGTTCTATCAGGCAGGAAATCACTTACACCGTTTGAATCAGGCAAGTACGAGTCACCCCCTACGAGTCAACCCCTTTCCCAGTGGGATTTATACTTTTATTTTTCAATCAACCAACCAACTTACC

>TRINITY_DN109116_c0_g1_i12 from sample A5 / Example unspliced isoform:

GCGGTCAGCCAGCCTTCCGgtaaacaagtttcatcctcttaccttcatttcgtattatattaaatggagggttgcagaggaataacggtttggagtttataaatctctgtttcttcgcagTCCCTGCGACCATGGATGACGTATAACAATGGATTGCGTACGACTTGGGTTTGGGTTTGCGCTCGAAACCGACGTGGAGG
ATAATGGATGACATAGCTGATGGCGAAAACTGTACTGCCAGCAGATTGTTGATGTCTTCGCAGGTTTCACTGCAGCAGCTACGAGTCTCCCTGGACCATGGCACTGAAAAAGATAGACAATGTGATACTCCAGGCGAGTCCAGGCCCAAGCTACCTTTTGAAATATGTGTAGAGCTCATGCTAAGATTTCTTATGTGTGG
TGCCTGGTTTGAATATTCTGTTAAATTGGTTAATTTGTTATGTAGAGATCTGTATGATGGTTGTTCACATTCAATGGCTTTGAAATGGATATTGGATTCATTACCCACGTTTTATTTTTGG