sp|Q67ZB3|FRL3_ARATH ----> FRIGIDA-like protein 3

Intron 1 GCACAGAGAA ... TGACAAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.58	89	12	43
A2	42.86	116	19	87
A3	39.52	101	27	66
A4	47.06	18	3	16
A5	44.23	29	11	23
A6	77.78	8	6	28
A7	53.82	242	37	282
A8	49.89	236	32	235
A9	59.05	163	34	235
A10	47.49	209	30	189

>TRINITY_DN45544_c0_g1_i28 from sample A7 / Example spliced isoform

CGGAGAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGACCGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAAACCGAAAGAAGGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGACCGAAAGAAGGCTGCTGAGAAAAGGGCACAGGAGAAAAACCACAGAAAAATACCTGGAATGTTAGAAAGGGTTA
TAGACGACAGGGAAGCTGCTAAGGAAAAGTCAAAGCAGAAAGATAATGAAGAGAAAGCTGCTGAGGAAAAGGCACAGGAGAAAGAAGACGGAAAGAAAGCTGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAATGAGAAAAAGGCACAGGAGAAAGAAGAGCACAGGAGAGAGAAGAGGGAAAGAAAGCTGCTG
AGATAAAGGCACAGGAAAAAGAAGAGGGAAATAAAACTGCTGAGAAAAATGCACAGGAGAAAGAAGATGGAAAGAAAGCTACTGCACAGGAGAAAAAAGACGGAGAGAGAGCTGCTGAGAAAAAGGCACAGGAGAAAGATGGAAAGATCGTGCAAAAGAAGATGGACAGAAAGCTGCTGAAAAAGGCACAGGTGAAAGAC
AGGAGAAAGAAGATGGAGGCACAGGAGAAAGAAGATGG

>TRINITY_DN45544_c0_g1_i31 from sample A7 / Example unspliced isoform:

AAAAAGGCACAGGTGAAAGAAGATGGAATGAAAGCTGCTGAGAAAAATGCACAGGTGAAAAAATACAGAAGGAAAGCTGCTGAGAAAGAGGCACAGGAGAAAGGAAATGGCGGAGAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGACCGAAAGAAAGCTGCTGAGAAAAAGgcacagagaaagctcctgagaaa
aaggcagaggagaaagaagaagaccgaaagaaggctgctgagaaaagggcacaggagaaatgctgagaaaaaggcacaggagaaagaagatggaaagaaaggtggtgacaaaaagGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGAAAAGACTGGAAAGAAAGCTGCTGAGA
AAAAGGCACAGGTAGAGAGAAGAGGGAAAGAAAGCTGCTGAGATAAAGGCACAGGAAAAAGAAGAGGGAAATAAAACTGCTGAGAAAAATGCACAGGAGAAAGAAGATGGAAAGAAAGCTACTGCACAGGAGAAAAAAGACGGAGAGAGAGCTGCTGAGAAAAAGGCACAGGAGAAAGATGGAAAGATAGACGGAGAGAA
AGCTGCTGAGAAAAAGGCACGTGCAAAAGAAGATGGACAGAAAGCTGCTGCTGAGAAAGGCACAGGTGAAAGAAGATGGAATGAAAGCAAGGCACAGGAGAAAGAAGATGGACTGCTGAGAACAAGGCACAGGCGAAAGAAGATGGAAAACGAAAGATGCTGAGAAAAAGGCACAGGCGAAAGAAGATGGGAAAGCTGCT
GAGAACGAAAGATGCTGAGAAAAAGGCACAGGCGAAAAAATACAGAAGTAAAGCTGCTGAAAAAGAGGCACAGGAGAAAGAAGACAGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAATGAAAGATGGAAAGATAGCTACTGAAAGGAAGATGG

Intron 2 GCTGCTGAGA ... AAAGGCAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	28	70
A2	97.24	2	36	105
A3	96.75	2	30	89
A4	100	0	3	18
A5	100	0	4	22
A6	100	0	4	6
A7	98.04	3	86	214
A8	98.99	2	103	289
A9	99.29	1	78	203
A10	95.92	7	82	247

>TRINITY_DN45544_c0_g1_i32 from sample A7 / Example spliced isoform

GAAGAAGACCGAAAGAAGGCTGCTGAGAAAAGGGCACAGGAGAAAGAAGACGGAGAGAAACCTTATGAGGAGGCACAGGAGAAAGAAGGTGGAGAGAAAGCTGGTGAGAAGAAGGCACAGGAGAAAGAAGACAGAGAGAAAGCTGGTTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGGTGGTGACAAAA
AGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAGAGAAGAGGGAAAGAAAGCTGCTGAGATAAAGGCACAGGAAAAAGAAGAGGGAAATAAAACTGCTGAGAAAAATGCACAGGAGAAAGAAGATGGAAAGAAAGCT
ACTGCACAGGAGAAAAAAGACGGAGAGAGAGCTGCTGAGAAAAAGGCACAGGAGAAAGATGGAAAGATCGTGCAAAAGAAGATGGACAGAAAGCTGCTGCTGAGAAAGGCACAGGTGAAAGAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGCAAAAGGCACAGGAGAAAGAAGACAGAAAGGAAGCTTCT
GAGAAAAAGGCACAGAAAGCTGCTGAGCATAGAAGACGTCAATCTTTTGAGCCTGGCTATGGGAAAAACTTTCAGAGAGGGAAAATTATGCTTCAAAGAGGACTAAGCATGCTTCACCGAACGAGACAAGCAGAGTGGCTGCTGGTAGTGACATCTCAAAAGGCTCACCCTATAATATCGATGGGGTCGAAAATAAGTCT
GGGATTGCTTCTGAGAAAAAGGCACAGGGCACAGGTATAAAAGACGTCAATCTTTTGAGAGAAACGAAGATGGCTGGCTATGGGCTTTCAGAGAGGGAGAACTATGCTTCAAAGAGGGCTGAGCATGCTTCACCGAACGAGACAATTAGAGTGGCT

>TRINITY_DN45544_c0_g1_i22 from sample A7 / Example unspliced isoform:

GAAGAAGACCGAAAGAAGGCTGCTGAGAAAAGGGCACAGGAGAAAGAAGACGGAGAGAAACCTTATGAGGAGGCACAGGAGAAAGAAGGTGGAGAGAAAGCTGGTGAGAAGAAGGCACAGGAGAAAGAAGACAGAGAGAAAGCTGGTTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGGTGGTGACAAAA
AGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAGAGAAGAGGGAAAGAAAGCTGCTGAGATAAAGGCACAGGAAAAAGAAGAGGGAAATAAAACTGCTGAGAAAAATGCACAGGAGAAAGAAGATGGAAAGAAAGCT
ACTGCACAGGAGAAAAAAGACGGAGAGAGAGCTGCTGAGAAAAAGGCACAGGAGAAAGATGGAAAGATCgctgctgagaaaaacgcacaggagaaaaaagacggacagaaagctgctgagaaaaaggcatgtgcaaaagaagatggacagaaagctgctgagaaaaaggcagagGTGCAAAAGAAGATGGACAGAAAGCT
GCTGAGAAAAAGGCAGAGGCAAAAGATGGACAGAAAGCTGCTGAGAAAAAGGCAGAGGCGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAGGCACAGGTGAAAGAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGCAAAAGGCACAGGAGAAAGAAGACAGAAAGAAAGCTGCTGAGAAAAAGGCACAGG
AGAAAGAAGATGG

Intron 3 GTTTTTGCTG ... TGTGTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.77	82	26	17
A2	20.62	77	22	18
A3	20.34	47	20	4
A4	11.39	35	3	6
A5	5.26	18	2	0
A6	23.08	10	3	3
A7	16.98	66	6	21
A8	18.18	45	7	13
A9	19.67	49	6	18
A10	16.08	60	5	18

>TRINITY_DN115993_c2_g7_i2 from sample A1 / Example spliced isoform

GCTGTCAAAGGGGAACCTGTCAAAGGGGCAACCTTGAACTTATGTGGCTTACACCACCGTATGTATTTTTTTTGGTCAGCCGACTCCCCGAGATTAGATAAACCTGAGCTTACATGATGGGCCCATGGGCTCTACTAAATGGCATTGAGGCCTATATATGCACACATTACTTCTCTTGTATTCACAGTACAAACAAATTC
ATTTTCCTCTTATGAGGGTTGCTTTGCTCTTGGGGTTTCAGAACAGTATCAATGACGGCAGTAAATCCGTGTTCGAATCTCTCCAACGCCTTCACTCTATGATTGAATCCTTCGACGAGTCTCTAACCCAGCGCTGTGCAAAGACAACAGCAGTGAGTCAGCCCTCGACCTGCGGAAGAACCTAATGTAGTATTGCCACC
GCCTCCACAAAGCCTTGCATTAAAGGTACGAAGCCCTAAAGATAAAGGATAAAAACCAGAAGAATCCGCAACCATGGGCAAAGACAAAGGGAAAAAAGGATCGAATGTCGAAGTCAACTCTAAGCTCAAAACCCTCTGCGATAAAGGCAACAGCCGTGGTCTCATACGTTACATCATCAGTAACATGAACGGGGTTCAAA
CCCTTCGGAATGAATTGAAAGTGGCGCTCAGTAACGCCCCCGATGCGGCGAAGCTGGTGATTAAGGCTCTGGAGCGCTATGATTTCTCAGATGATTCCAATAGAAGGGCTTGCATTTTGCTCTTGGAATCCCTTTTCCCCGTTTTTGGTAACGACGGCAACGCTGCTGTTCCATCGGATGTCAAGAATTCGGCCCGGGAA
ATTGCTAAGGCTTGGAGAGAAGGGATGGCTAAGGTTTGGAGAGAAGGGATGGATACGAACTGTAGTTTGGATAGCCAGGCTTTCCTGCAGCTCTTGGTCTCGTTTAGGATCGCGGCGGACTACGAGAAGGATGAGCTTTTGGGGATACTGTTTCATATGTCGAGCCACAAGCATGTTCCAGAGCTGTTTCGGTCTCTTGG
GTTGTCTCCCGACGTAGATTTTATCAGTAAGGTTTCCAAAAATCGTGGACCGTTAATTGCCCTTAGACTCGCCCATACATTTGGCATGATGGATAAAGTACATCCTGTGGGTTTGTTGACATCACATTTGAGAGATGTGGATGAGTACATTGCGAAGGGACTTGTGACGAGAGCATGTGGCACGGATGGATCTAAGCCAC
AAAATCAAATGTACAGACTCTGGAGGAATGCAAATTCTAAGAAGATTGGTGTTTTGAGTAGTGTTCTTAATCTTGTTGAAATGTATGGTTTGCAATGTGATTCTTTCAAAGCAAACTTGCGGAAGGAACTAAGAAACTTGGAACAGGAGAAAGTAGATGGATTAGAAACGATAAGAGCTGGGAAAATCAAAGCTTCTGAG
ATAAAGGCAAAGCAGAAAGGAGAGAGAACTGATGAGAAAAAGGCACAGGAGAAAACAAATGGAGAGAAAGCTGCCGAGAAAAAGGCACAGGCAAAAGAAGATGGAGAGAAAGATGCTGAGAAAAAGGCACAGGCGAAA

>TRINITY_DN115993_c2_g7_i4 from sample A1 / Example unspliced isoform:

GCTGTCAAAGGGGAACCTGTCAAAGGGGCAACCTTGAACTTATGTGGCTTACACCACCGTATGTATTTTTTTTGGTCAGCCGACTCCCCGAGATTAGATAAACCTGAGCTTACATGATGGGCCCATGGGCTCTACTAAATGGCATTGAGGCCTATATATGCACACATTACTTCTCTTGTATTCACAGTACAAACAAATTC
ATTTTCCTCTTATGAGGGTTGCTTTGCTCTTGGGGTTTCAGAACAGTATCAATGACGGCAGTAAATCCGTGTTCGAATCTCTCCAACGCCTTCACTCTATGATTGAATCCTTCGACGAGTCTCTAACCCAGCGCTGTGCAAAGACAACAGCAGTGAGTCAGCCCTCGACCTGCGGAAGAACCTAATGTAGTATTGCCACC
GCCTCCACAAAGCCTTGCATTAAAGGTACGAAGCCCTAAAGATAAAGGATAAAAACCAGAAGAATCCGCAACCATGGGCAAAGACAAAGGGAAAAAAGGATCGAATGTCGAAGTCAACTCTAAGCTCAAAACCCTCTGCGATAAAGGCAACAGCCGTGGTCTCATACGTTACATCATCAGTAACATGAACGGGGTTCAAA
CCCTTCGGAATGAATTGAAAGTGGCGCTCAGTAACGCCCCCGATGCGGCGAAGCTGGTGATTAAGGCTCTGGAGCGCTATGATTTCTCAGATGATTCCAATAGAAGGGCTTGCATTTTGCTCTTGGAATCCCTTTTCCCCGTTTTTGGTAACGACGGCAACGCTGCTGTTCCATCGGATGTCAAGAATTCGGCCCGGGAA
ATTGCTAAGGCTTGGAGAGAAGGGATGGCTAAGGTTTGGAGAGAAGGGATGGATACGAACTGTAGTTTGGATAGCCAGGCTTTCCTGCAGCTCTTGGTCTCGTTTAGGATCGCGGCGGACTACGAGAAGGATGAGCTTTTGGGGATACTGTTTCATATGTCGAGCCACAAGCATGTTCCAGAGCTGTTTCGGTCTCTTGG
GTTGTCTCCCGACGTAGATTTTATCAGTAAGGTTTCCAAAAATCGTGGACCGTTAATTGCCCTTAGACTCGCCCATACATTTGGCATGATGGATAAAGTACATCCTGTGGGTTTGTTGACATCACATTTGAGAGATGTGGATGAGTACATTGCGAAGGGACTTGTGACGAGAGCATGTGGCACGGATGGATCTAAGgttt
ttgctgatgtttatatcttttttttttaagattgaaagtgacttaatgtaacactgaatcgaaaatttattttctgatttgtgttgtagCCACAAAATCAAATGTACAGACTCTGGAGGAATGCAAATTCTAAGAAGATTGGTGTTTTGAGTAGTGTTCTTAATCTTGTTGAAATGTATGGTTTGCAATGTGATTCTTTC
AAAGCAAACTTGCGGAAGGAACTAAGAAACTTGGAACAGGAGAAAGTAGATGGATTAGAAACGATAAGAGCTGGGAAAATCAAAGCTTCTGAGATAAAGGCAAAGCAGAAAGGAGAGAGAACTGATGAGAAAAAGGCACAGGAGAAAACAAATGGAGAGAAAGCTGCCGAGAAAAAGGCACAGGCAAAAGAAGATGGAGA
GAAAGATGCTGAGAAAAAGGCACAGGCGAAA

Intron 4 GCACAGGAGA ... GGAGAGAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60.93	189.5	289	302
A2	63	252	429	429
A3	63.79	212	383	364
A4	62.81	53	89	90
A5	65.45	71	140	129
A6	64.47	70	132	122
A7	69	663	1486	1465
A8	66.85	635	1269	1292
A9	67.94	561	1196	1182
A10	65.8	653.5	1275	1240

>TRINITY_DN45544_c0_g1_i32 from sample A7 / Example spliced isoform

GAAGAAGACCGAAAGAAGGCTGCTGAGAAAAGGGCACAGGAGAAAGAAGACGGAGAGAAACCTTATGAGGAGGCACAGGAGAAAGAAGGTGGAGAGAAAGCTGGTGAGAAGAAGGCACAGGAGAAAGAAGACAGAGAGAAAGCTGGTTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGGTGGTGACAAAA
AGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAGAGAAGAGGGAAAGAAAGCTGCTGAGATAAAGGCACAGGAAAAAGAAGAGGGAAATAAAACTGCTGAGAAAAATGCACAGGAGAAAGAAGATGGAAAGAAAGCT
ACTGCACAGGAGAAAAAAGACGGAGAGAGAGCTGCTGAGAAAAAGGCACAGGAGAAAGATGGAAAGATCGTGCAAAAGAAGATGGACAGAAAGCTGCTGCTGAGAAAGGCACAGGTGAAAGAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGCAAAAGGCACAGGAGAAAGAAGACAGAAAGGAAGCTTCT
GAGAAAAAGGCACAGAAAGCTGCTGAGCATAGAAGACGTCAATCTTTTGAGCCTGGCTATGGGAAAAACTTTCAGAGAGGGAAAATTATGCTTCAAAGAGGACTAAGCATGCTTCACCGAACGAGACAAGCAGAGTGGCTGCTGGTAGTGACATCTCAAAAGGCTCACCCTATAATATCGATGGGGTCGAAAATAAGTCT
GGGATTGCTTCTGAGAAAAAGGCACAGGGCACAGGTATAAAAGACGTCAATCTTTTGAGAGAAACGAAGATGGCTGGCTATGGGCTTTCAGAGAGGGAGAACTATGCTTCAAAGAGGGCTGAGCATGCTTCACCGAACGAGACAATTAGAGTGGCT

>TRINITY_DN45544_c0_g1_i33 from sample A7 / Example unspliced isoform:

CGGAGAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGACCGAAAGAAAGCTGCTGAGAAAAAGGCAAAAAGGCACAGGAGAAAGAAGAGGGAAATAAAGGACAGGAGAAAGAAGAAGACCGAAAGAAGGCTGCTGAGAAAAGGGCACAGGAGAAAGAAGACGGAGAGAAACCTTATGAGGAGGCACAGGAGAAAGA
AGGTGGAGAGAAAGCTGGTGAGAAGAAGGCACAGGAGAAAGAAGACAGAGAGAAAGCTGGTTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGGTGGTGACAAAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGgcacaggagaaagaagagggaaataaagctgcggagagaaag


GCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAAAGTCCCAGGAGAAAGAAGAGAGAAATAAAGCTGCTGAGAAAAAGGCACAGGAGAGAGAAGAGGGAAAGAAAGCTGCTGAGATAAAGGCACAGGAAAAAGAAGAGGGAAATAAAACTGCTGAGAAAAATGCACAGGAGAAAGAAGATGGAAAGAAAGCTAC
TGCACAGGAGAAAAAAGACGGAGAGAGAGCTGCTGAGAAAAAGGCACAGGAGAAAGTATGGAAAGATAGACGGAGAGAAAGCTGCTGAGAAAAAGGCACGGCATGTGCAAAAGAAGATGGACAGAAAGCTGCTGAGAAAAAGGCAGAGGTGCAAAAGAAGATGGACAGAAAGCTGCTGAGAAAAAGGCAGAGGCAAAAGA
TGGACAGAAAGCTGCTGAGAAAAAGGCAGAGGCGAAAGAAGATGGAAAGAAAGCTGCTGAGAAAGGCACAGGTGAAAGAAAGGCACAGGAGAAAGAAGATGGAAAGAAAGCTGCTGAGCAAAAGGCACAGGAGAAAGAAGACAGAAAGAAAGCTGCTGAGAAAAAGGCACAGGAGAAAGAAGATGG