sp|Q8L7K4|HSCB_ARATH ----> Iron-sulfur cluster co-chaperone protein HscB homolog

Intron 1 GTATGTTGAT ... CCTATGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.06	39	25	2.3
A2	22.22	21	6	2
A3	15.79	48	9	0
A4	29.41	24	10	0.7
A5	25	6	2	0
A6	22.39	52	15	4
A7	31.5	87	40	8
A8	27.03	108	40	10
A9	27.01	100	37	16.3
A10	20.78	61	16	5.3

>TRINITY_DN42279_c0_g2_i1 from sample A7 / Example spliced isoform

GCCGTAAAAGGATATGCCAGTCCGTGTTTTCAAAGTATGCCCAGGGGCTTTCCTCTACCTAAATGCAAGGATTGGAGGCATTCATTCCGCCATTAGAGTGGGACCTGTACTCAACCCCATCTCAGTCAGACAGATCAAGGGGAGAATGGAACTATATGAGTGTTTACATGCATGACTGAAAACTCGAGACGCGTGGAGCG
AGTTCAACCCTGCTTTATCAACCTACAAACTCTCTTCATCCTCTCTTCTTCCCTTGAGCTCCTGGCAGAGCGAGAGACCGAATTCGGTTGATAATTCATCAGGGGATTATTGTCGGTTGTAGAGAACCCTCCTTAAGGAAACGCCTTTCGGCCTAATATCAAAACAACCTAAACTGGAGGAAGCTTCTCATTCTGGCTGC
AACTATGGTGGTGAGGAGGAGGCTTCTCAATCTAGCTGCAACTTTAACTCATGATACCCAATTCTCGTTAACTTCTCCCAATCAGGCCTTCGATGCCTTTCTTCACCAAGCTTCATCAGGGTTTCAGCCTTTATCTAATTCGGTCCGTTCTAGCTGCCCAAATGTTTTTAACAAAGAAAACTGCATACCTAACTGCCCCA
GTGGCCTTCTCCAGCATTTGACCAACTTTGGCAGGTTTTCCTCCTCGGCAAATACTCACGATGCTGTCATTAAATGCTGGGCCTGTGCCGCGGAGGCACCTGATGGGCCATTTCTGGTGTGCCCATCATGCAAAAGTGTTCAACCCCTCGATTCTTCAGTCAACTATTTCCAAATATTCAATCTAGAGCCAGGTTATGAT
GTGTCTACCAACGATCTGGAAAGAAAGTACAAAGTTTGGCAGAAGAAATTGCATCCCGATTTATTTCAGTGCAACTCTGAGCAAGAGAAGGAGAATTCAGCTCAGCAATCTGCCCAGGTGATCAAGGCTTATAATGTGCTTCTTAAGCCTCTGTCCAGAGCAGAATATCTGTTGCAACTTCAAGGTATCCATGTTGATGA
GGAAGGAACTGTCACTGATCCAGAATTGCTGATGGAGATCATGGAGGTTCGAGAATCTGTAGAAGATGCCAACAATGCTGATACTCTCAAAACACTTCAGAGTAAAATACAAGCTAAACTTGAAGAATGCCGAACATCTTTCTCGTCTGCATACGAGAGACATGATTTTGCCTCTGCAGTGTCATCTGTTCAGAAGATGT
CATATTATGAACGTACAAATGCAGAGATCATTAAGAAGCTTTGAATGGCTTGATAATAATTAACATGGTGAGAGTTTTTAGTCAGTGGAATTGATTGCTCAGTGTTCTTCACATTTAACCCTCTTTGTGGGTGTTGGATTTTACTTCCGAGGGACGAAGTTTCATATTCTAAAGCAATTACCTATGGAAAGCAGGTGAAA
ATCAGAGAACTGAAGGTCCACCCTCGTGTAAATCCAGTTGGAATAGTGACTTGGATCTAAAAGAATTGTCAGCAAATTCATCTTTTCGATATAACAATTATTGAACTAGGTTTTTGGGTCATAAATACCCAAGAAGCCAGTCATATCACAATAAGTAAGAATTAGTACCTCTAAATTCAAGGTACGCGTAAAATAAAAAT
ATTGCAAGTTTGCCTCTCTAGGTTCTTTGATTACCTTGCTGATACAAACTATTATCATAAAATGCCTTTTACAGAGGCAGGTATTAATTTGATTATATTGAATATATAGAAATTGTATGCGACAAATGTCTATTTCCCCTGCCTTGCGAGCAAGGGACTGTGTGGACATGTTTTATTTTTATTTTTTGCCTAGTGTATAG
AGAATTCAAG

>TRINITY_DN42279_c0_g2_i3 from sample A7 / Example unspliced isoform:

GCCGTAAAAGGATATGCCAGTCCGTGTTTTCAAAGTATGCCCAGGGGCTTTCCTCTACCTAAATGCAAGGATTGGAGGCATTCATTCCGCCATTAGAGTGGGACCTGTACTCAACCCCATCTCAGTCAGACAGATCAAGGGGAGAATGGAACTATATGAGTGTTTACATGCATGACTGAAAACTCGAGACGCGTGGAGCG
AGTTCAACCCTGCTTTATCAACCTACAAACTCTCTTCATCCTCTCTTCTTCCCTTGAGCTCCTGGCAGAGCGAGAGACCGAATTCGGTTGATAATTCATCAGGGGATTATTGTCGGTTGTAGAGAACCCTCCTTAAGGAAACGCCTTTCGGCCTAATATCAAAACAACCTAAACTGGAGGAAGCTTCTCATTCTGGCTGC
AACTATGGTGGTGAGGAGGAGGCTTCTCAATCTAGCTGCAACTTTAACTCATGATACCCAATTCTCGTTAACTTCTCCCAATCAGGCCTTCGATGCCTTTCTTCACCAAGCTTCATCAGGGTTTCAGCCTTTATCTAATTCGGTCCGTTCTAGCTGCCCAAATGTTTTTAACAAAGAAAACTGCATACCTAACTGCCCCA
GTGGCCTTCTCCAGCATTTGACCAACTTTGGCAGGTTTTCCTCCTCGGCAAATACTCACGATGCTGTCATTAAATGCTGGGCCTGTGCCGCGGAGGCACCTGATGGGCCATTTCTGGTGTGCCCATCATGCAAAAGTGTTCAACCCCTCGATTCTTCAGTCAACTATTTCCAAATATTCAATCTAGAGCCAGGTTATGAT
GTGTCTACCAACGATCTGGAAAGAAAGTACAAAGTTTGGCAGAAGAAATTGCATCCCGATTTATTTCAGTGCAACTCTGAGCAAGAGAAGGAGAATTCAGCTCAGCAATCTGCCCAGGTGATCAAGGCTTATAATGTGCTTCTTAAGCCTCTGTCCAGAGCAGAATATCTGTTGCAACTTCAAGGTATCCATGTTGATGA
GGAAGGAACTGTCACTGATCCAGAATTGCTGATGGAGATCATGGAGGTTCGAGAATCTGTAGAAGATGCCAACAATGCTGATACTCTCAAAACACTTCAGAGTAAAgtatgttgatcatgttattttagatttatgtatcatgttgaatttcattatttctttttgttcccaagtacaattgattttcctatgccagATA
CAAGCTAAACTTGAAGAATG

Intron 2 GTGAGAGTTT ... TGGAAAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.75	8	32	31
A2	78.26	5	25	11
A3	61.29	12	16	22
A4	70.73	6	22	7
A5	70	3	8	6
A6	73.07	23	47	77.8
A7	63.23	41	65	76
A8	68.06	46	96	100
A9	64.38	39	52	89
A10	70.64	16	34	43

>TRINITY_DN107644_c0_g1_i3 from sample A3 / Example spliced isoform

GGAAAAGGATATGCCAGTCCGTGTTTTCAAAGTATGCCCAGGGGCTTTCCTCTACCTAAATGCAAGGATTGGAGGCATTCATTCCGCCATTAGAGTGGGACCTGTACTCAACCCCATCTCAGTCAGACAGATCAAGGGGAGAATGGAACTATATGAGTGTTTACATGCATGACTGAAAACTCGAGACGCGTGGAGCGAGT
TCAACCCTGCTTTATCAACCTACAAACTCTCTTCATCCTCTCTTCTTCCCTTGAGCTCCTGGCAGAGCGAGAGACCGAATTCGGTTGATAATTCATCAGGGGATTATTGTCGGTTGTAGAGAACCCTCCTTAAGGAAACGCCTTTCGGCCTAATATCAAAACAACCTAAACTGGAGGAAGCTTCTCATTCTGGCTGCAAC
TATGGTGGTGAGGAGGAGGCTTCTCAATCTAGCTGCAACTTTAACTCATGATACCCAATTCTCGTTAACTTCTCCCAATCAGGCCTTCGATGCCTTTCTTCACCAAGCTTCATCAGGGTTTCAGCCTTTATCTAATTCGGTCCGTTCTAGCTGCCCAAATGTTTTTAACAAAGAAAACTGCATACCTAACTGCCCCAGTG
GCCTTCTCCAGCATTTGACCAACTTTGGCAGGTTTTCCTCCTCGGCAAATACTCACGATGCTATCATTAAATGCTGGGCCTGTGCCGCGGAGGCACCTGATGGGCCATTTCTGGTGTGCCCATCATGCAAAAGTGTTCAACCCCTCGATTCTTCAGTCAACTATTTCCAAATATTCAATCTAGAGCCAGGTTATGATGTG
TCTACCAACGATCTGGAAAGAAAGTACAAAGTTTGGCAGAAGAAATTGCATCCTGATTTATTTCAGTGCAACTCCGAGCAAGAGAAGGAGAATTCAGCTCAGCAATCTGCCCAGGTGATCAAGGCTTATAATGTGCTTCTTAAGCCTCTGTCCAGAGCAGAATATCTGTTGCAACTTCAAGGTATCCATGTTGATGAGGA
AGGAACTGTCACTGATCCAGAATTGCTGATGGAGATCATGGAGGTTCGAGAATCTGTAGAAGATGCCAACAATGCTGATACTCTCAAAACACTTCAGAGTAAAATACAAGCTAAACTTGAAGAATGCCGAACATCTTTCTCATCTGCATACGAGAGACATGATTTTGCCTCTGCAGTGTCATCTGTTCAGAAGATGTCAT
ATTATGAACGTACAAATGCAGAGATCATTAAGAAGCTTTGAATGGCTTGATAATAATTAACATGGTGAAAATCAGAGAACTGAAGGTCCACCCTCGTGTAAATCCAGTTGGAATAGTGACTTGGATCTAAAAGAATTGTCAGCAAATTCATCTTTTCGATATAACAATTATTGAACTAGGTTTTTGGGTCATAAATACCC
AAGAAGCCAGTCATATCACAATAAGTAAGAATTAGTACCTCTAAATTCAAGGTACGCGTAAAATAAAAATATTGCAAGTTTGCCTCTCTAGGTTCTTTGATTACCTTGCTGATACAAACTATTATCATAAAATGCCTTTTACAGAGGCAGGTATTAATTTGATTATATTGAATATATAGAAATTGTATGCGACAAATGTC
TATTTCCCCTGCCTTGCGAGCAAGGGACTGTGTGGACATGTTTTATTTTTATTTTTTGCCTAG

>TRINITY_DN107644_c0_g1_i2 from sample A3 / Example unspliced isoform:

GGAAAAGGATATGCCAGTCCGTGTTTTCAAAGTATGCCCAGGGGCTTTCCTCTACCTAAATGCAAGGATTGGAGGCATTCATTCCGCCATTAGAGTGGGACCTGTACTCAACCCCATCTCAGTCAGACAGATCAAGGGGAGAATGGAACTATATGAGTGTTTACATGCATGACTGAAAACTCGAGACGCGTGGAGCGAGT
TCAACCCTGCTTTATCAACCTACAAACTCTCTTCATCCTCTCTTCTTCCCTTGAGCTCCTGGCAGAGCGAGAGACCGAATTCGGTTGATAATTCATCAGGGGATTATTGTCGGTTGTAGAGAACCCTCCTTAAGGAAACGCCTTTCGGCCTAATATCAAAACAACCTAAACTGGAGGAAGCTTCTCATTCTGGCTGCAAC
TATGGTGGTGAGGAGGAGGCTTCTCAATCTAGCTGCAACTTTAACTCATGATACCCAATTCTCGTTAACTTCTCCCAATCAGGCCTTCGATGCCTTTCTTCACCAAGCTTCATCAGGGTTTCAGCCTTTATCTAATTCGGTCCGTTCTAGCTGCCCAAATGTTTTTAACAAAGAAAACTGCATACCTAACTGCCCCAGTG
GCCTTCTCCAGCATTTGACCAACTTTGGCAGGTTTTCCTCCTCGGCAAATACTCACGATGCTATCATTAAATGCTGGGCCTGTGCCGCGGAGGCACCTGATGGGCCATTTCTGGTGTGCCCATCATGCAAAAGTGTTCAACCCCTCGATTCTTCAGTCAACTATTTCCAAATATTCAATCTAGAGCCAGGTTATGATGTG
TCTACCAACGATCTGGAAAGAAAGTACAAAGTTTGGCAGAAGAAATTGCATCCTGATTTATTTCAGTGCAACTCCGAGCAAGAGAAGGAGAATTCAGCTCAGCAATCTGCCCAGGTGATCAAGGCTTATAATGTGCTTCTTAAGCCTCTGTCCAGAGCAGAATATCTGTTGCAACTTCAAGGTATCCATGTTGATGAGGA
AGGAACTGTCACTGATCCAGAATTGCTGATGGAGATCATGGAGGTTCGAGAATCTGTAGAAGATGCCAACAATGCTGATACTCTCAAAACACTTCAGAGTAAAATACAAGCTAAACTTGAAGAATGCCGAACATCTTTCTCATCTGCATACGAGAGACATGATTTTGCCTCTGCAGTGTCATCTGTTCAGAAGATGTCAT
ATTATGAACGTACAAATGCAGAGATCATTAAGAAGCTTTGAATGGCTTGATAATAATTAACATGgtgagagtttttagtcagtggaattgattgctcagtgttcttcacatttaaccctctttgtgggtgttggattttacttccgagggacgaagtttcatattctaaagcaattacctatggaaagcagGTGAAAATC
AGAGAACTGAAGGTCCACCCTCGTGTAAATCCAGTTGGAATAGTGACTTGGATCTAAAAGAATTGTCAGCAAATTCATCTTTTCGATATAACAATTATTGAACTAGGTTTTTGGGTCATAAATACCCAAGAAGCCAGTCATATCACAATAAGTAAGAATTAGTACCTCTAAATTCAAGGTACGCGTAAAATAAAAATATT
GCAAGTTTGCCTCTCTAGGTTCTTTGATTACCTTGCTGATACAAACTATTATCATAAAATGCCTTTTACAGAGGCAGGTATTAATTTGATTATATTGAATATATAGAAATTGTATGCGACAAATGTCTATTTCCCCTGCCTTGCGAGCAAGGGACTGTGTGGACATGTTTTATTTTTATTTTTTGCCTAG