sp|F4IS91|RSA1_ARATH ----> Protein SHORT ROOT IN SALT MEDIUM 1


Intron 1 GTGAGACTGT ... ATATTGTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A124.28135.837.543
A223.7112135.537
A329.6814431.760
A432.68129.233.662
A524.5363.420.420
A633.33201
A719.36212.85
A8302.61.41
A926.675.61.42
A1027.4910.61.64



>TRINITY_DN120287_c9_g1_i7 from sample A2 / Example spliced isoform
TACTCACTGCTTACAACTCACTAGTCACTGCTTACAACTGCTTACAACTCACTACTCACTGCTTACAACTCACTAGTCACTGCTTACAACTGCTTACAACTCACTACTCACTGCTTACAACTCACTACTCACAGCTATCAACCAACTCACACTCAACCAATGCAACCAACTCACAGCTCTCAACCACTTGAGCTAACCCA
CCAGGCTCTTTTCTTTTTATATGCCAGTCTTATAATGTAGTTATGTAAAATATGCAAAATTTGTGTCATAATAATCTGCTATTTTAAAAAATTCCCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAAT
GCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTC
TGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGT
ATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCTGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCA
CAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGA
TCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAG
AGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAG
AGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCT
TGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTG
ATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGC
TGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAA
GGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAG
CAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATC
ATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAATTAAGAAAACAGAGAAGAAGAAAGATGAGAAG


>TRINITY_DN120287_c9_g1_i13 from sample A2 / Example unspliced isoform:
CGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTCATCCTTCTACATTT
CTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATTTCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGGGCAACAGgtgagac
tgtttgtgcctatttaagcatccttgaattcttaattttttgggaacaaattttttggataacactcagcataccaacatgaatggggctcttttcaattcttgaagatttgcaaactcttgcctgtgcaaattatttattgcaactgataaatgtttgaacaaatttaaaatttatattgtcagGCTGCAACTGCATAT
GCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAACAGGTAATTACTCTAATAATTTG
TACTTTAGTTCTCTAATAAATTTGAAGATGCATTTTCTTCAAAAACAAAGACTTGAAGGTTTCAAATATTTGGATAATTTTAAAATACCGACTTACGTCCCTTCTTCCATAATCCGTCATTATGCTCATGTTGCCCTCTAATTGCATTTTTATAGCTTGACCTTGATTAAAATTTGCCTTTTGATTGTGAATCGTCACTA
TCTGAATGTTTATGTTGTTTAATGGAGCTAGGAAGTATTATAGCAACTATTCTGTAAGATATTGTATTTGCATATATTTTGTCATCAACAAGGTACCGATGCAAGTGTGAATCTTATCCTTCAA



Intron 2 GTAATTACTC ... CTTCTCACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A115.56152228
A212.74137920
A316.72184.31037
A420.61131234
A513.85112118
A625301
A703100
A828.57502
A913.792504
A10162104



>TRINITY_DN108439_c0_g2_i4 from sample A4 / Example spliced isoform
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTC
ATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGG
GCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAAC
AGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCA
CCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATTTACAAGATCAGGCTCT
ATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTG
GTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGAT
ACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCG
TAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACATCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTA
AGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGTTTAATGTTGATAGGATATCGGCCA
AAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTGTCTGAGTTAATATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAACTCTTGATCTGAAATGGGATGCAA
CAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTCGGAGCTTCTATGATCATTTTGGAC
CATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAATGATAAAAATGCACCGATACGTTG
CTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTAATGAGTGTAAAGTACAAGCAAATC
TAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGA
GCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACA
GAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCG
GCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAA
GAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAGAAAGCAGAGAAAA
AGAAAGATGAGAAGATCACTGA


>TRINITY_DN108439_c0_g2_i12 from sample A4 / Example unspliced isoform:
CAACTCAAAGTGGTGCTCAACAGgtaattactctaataatttgtactttagttctctaataaatttgaagatgcattttcttcaaaaacaaagacttgaaggtttcaaatatttggataattttaaaatactgacttacgtcccttctgccatgatccgtcattatgctcatgttgccctctatgtgaattgtgaattgt
cactatctgaatgtttatgttgtttaatggagctaggaagtattataacaactattctgtaagatattgtatttgcatatattttgtcatcaacaaggtaccgatgcaattgtgaatcttatccttcaactctgctgttgatattttccttgtcttatactatatagtatcaatttcataataataatgaccagtgaaac
gccttactcttctcacagCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGA
TTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATT
TACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGT
GGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTT
TGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGAC
CTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACATCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTA
GCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGTTTAATGTT
GATAGGATATCGGCCAAAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTGTCTGAGTTAATATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAACTCTTGAT
CTGAAATGGGATGCAACAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTCGGAGCTTC
TATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAATGATAAAA
ATGCACCGATACGTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTAATGAGTGT
AAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTT
CTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAA
GGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGA
AAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAA
AAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGAC
AAGAAAGCAGAGAAAAAGAAAGATGAGAAGATCACTGA



Intron 3 GTGAATTTGG ... GATTTTGTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A132.11148708.5
A240.58123848.7
A333.331185915.3
A436.11925212.2
A541.8893674.2
A641.67750
A737.535210.2
A835.4231170
A928.8932130.5
A1030.6543190.7



>TRINITY_DN120287_c9_g1_i3 from sample A2 / Example spliced isoform
TACTCACTGCTTACAACTCACTAGTCACTGCTTACAACTGCTTACAACTCACTACTCACTGCTTACAACTCACTAGTCACTGCTTACAACTGCTTACAACTCACTACTCACTGCTTACAACTCACTACTCACAGCTATCAACCAACTCACACTCAACCAATGCAACCAACTCACAGCTCTCAACCACTTGAGCTAACCCA
CCAGGCTCTTTTCTTTTTATATGCCAGTCTTATAATGTAGTTATGTAAAATATGCAAAATTTGTGTCATAATAATCTGCTATTTTAAAAAATTCCCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGGGCAACAGGTGAGACTGTTTGTGCCTATTTAA
GCATCCTTGAATTCTTAATTTTTTGGGAACAAATTTTTTGGATAACACTCAGCATACCAACATGAATGGGGCTCTTTTCAATTCTTGAAGATTTGCAAACTCTTGCCTGTGCAAATTATTTATTGCAACTGATAAATGTTTGAACAAATTTAAAATTTATATTGTCAGGCTGCAACTGCATATGCTGGCAATGCATCAGC
TAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTA
CTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGT
GGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCTGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACT
GCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGT
ATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGAT
TTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCC
TAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTA
CACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGT
TCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCG
CTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCG
ATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAA
AAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGA
CAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAATTAAGAAAACAGAGAAGAAGAAAGATGAGAAG


>TRINITY_DN120287_c9_g1_i1 from sample A2 / Example unspliced isoform:
TCAGGTATTTGGTATTTAAACTTGTCATTGTTATTGTACACTGTACAGATTTATGCAGTTGTGTCGTGTACCTATTTATGTTTTTGTTAATTAACATGCAGTGTTAAGTTTTATGCCTTTTACTGTGAATGTATAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGA
GACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCAT
GCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAG
GGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCC
GTGAGCCCATTCGCAGgtgaatttggtttgtttttattggtaatgctgctgctgcctatgttacttcgcattttgatgtttacctgtgtaatgattttgtagGCGCGTATCCCCGGTTAGAGAGTACATCTGCAAGGTAAATCTGAGTTCTGAATTATGATGCTGTCTTAATGTTCATAGAGGAGATCTATTTATCTTTA
CATGCGCTTTAAAATTCAAGATTCAAAATGATGAACCCTCTATATGAGTGTAGATAGAATCATTTCATGAAGAAATGCAATGCACTGTATTTGATCAATGCTAGATAGGGCTGCCCTTATTTTAGTCAACGTATTCATTATTTTAAGTTTTTTTTTGAAATGCCAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGG
ATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATT
AATGTATCTGGGATATGCTGCTGCC



Intron 4 GTAAATCTGA ... GAAATGCCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A130.8115.5549
A230.6354622
A331.1811234
A428.2766525
A535.6653.3128
A6-11.500
A738.226.8016
A834.5620.5010
A936.0933018
A1023.9125.307



>TRINITY_DN118139_c2_g1_i9 from sample A3 / Example spliced isoform
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTC
ATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGG
GCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAAC
AGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCA
CCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATTTACAAGATCAGGCTCT
ATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTG
GTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGAT
ACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCG
TAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTA
AGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATG
AGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTT
CAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGC
GTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTCGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAA
GATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAA
GAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAA


>TRINITY_DN118139_c2_g1_i6 from sample A3 / Example unspliced isoform:
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTC
ATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGG
GCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAAC
AGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCA
CCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATTTACAAGATCAGGCTCT
ATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTG
GTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGAT
ACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCG
TAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGTGAATTTGGTTTGTTTTTATTGGTAACGCTGCTGCTGCCTATGTTACTTCGCATTTTGATGTTTACCTGTGTAATGATTTTGTAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGgtaaa
tctgagttctgaattatgatgctgtcttaatgttcatagaggagatctatttatctttacatgcgctttaaaattcaagattcaaaatgatgaaccctctatatgagtgtagatagaatcatttcatgaagaaatgcaatgcactgtatttgatcaatgctagatagggctgcccttattttagtcaacgtattcattat
tttaagtttttttttttgaaatgccagATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTA
TTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGTTTAATGTTGATAGGATATCGGCCAAAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTGTCTGAGTTAAT
ATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAACTCTTGATCTGAAATGGGATGCAACAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGA
GGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTCGGAGCTTCTATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTT
ATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAATGATAAAAATGCACCGATACCTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAG
TTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTAATGAGTGTAAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGG
TCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATC
CGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCA
TAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTCGAAGGATGAGAAAAAGGAGAATACTGATA
ACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGAT
CAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAA



Intron 5 GTAATCTGTC ... TGTTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A127.41944130
A232.57591443
A332.47521931
A430.844820.822
A525.6429146
A624.321427
A725.71651332
A827.1451533
A925.89731239
A1029.240429



>TRINITY_DN125594_c6_g1_i9 from sample A1 / Example spliced isoform
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GGTACACCTTTTGGAGCTTTTGCAATCAGAATGTTTTAAGATTGTCTGGTAAAACACATCTTAAATGTTTATATTTAGATCATTTGTTGTCTTTGTTGAAATCTGAAAGATTTGTTTTTGATCTGCTGTGCTGCCTTGTGGATTTACTGCAGTCCGATTTACAAGATCAGGTATTTGGTATTTAAACTTGTCATTGTTAT
TGTACACTGTACAGATTTATGCAGTTGTGTCATGGTACCTATTTATGTTTTTGTTAATTAACATGCAGTGTTAAGTTTTATGCCTTTTACTGTGAATGTATAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGG
GTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGA
GATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACG
AGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTA
GAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGC
GTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCG
TAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAA
GCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATC
AACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGA
TGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAAAGAAAGA
TGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAA


>TRINITY_DN125594_c6_g1_i8 from sample A1 / Example unspliced isoform:
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GTCCGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAG
GACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATA
GATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCC
TGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGC
TCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGT
TTAATGTTGATAGGATATCGGCCAAAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTGTCTGAGTTAATATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAA
CTCTTGATCTGAAATGGGATGCAACAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGgtaatctgtcgagatgaggcgacttttcggtgttgcatcaacaaacgtcttc
ggagcttctatgatcattttggaccatagaaagtatatttttagaattttaggtggagtttattgcgtgatcctccttcagaggtttgagggccccccttcagtttatcattatcgaattactgttcgtgatgacagcggctgcgccatttactcttttagaagcaccacatagtattcatatacaccatgtttatataa
tgataaaaatgcaccgataccttgctgttttgctgttggtggtgggcgcggttgttggcgaaagcatgcgaagaagaacgttccaaagtttattctgctctttaagtttcgttagaaggagacacgcttgtgtgataaaagtgttatataggattatcatcttccgagtgatttgatctaattgattagtcttttttcta
atgagtgtaaagtacaagcaaatctaaccttgccgtaaataaatttatggtgtcatttgtactggttgatttattttgtactctcttacatgtaatgttttgcagGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCT
TAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAA
TAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCC
AAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAA
AGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCA
CTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAG
AAAGATGAGAAGAA



Intron 6 GTACACCTTT ... TTTACTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A114.552293912
A216.16166320
A314.63251433
A413.13139215
A512.6197143
A60400
A74.174620
A820.933490
A97.554941
A106.256040



>TRINITY_DN125594_c6_g1_i8 from sample A1 / Example spliced isoform
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GTCCGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAG
GACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATA
GATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCC
TGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGC
TCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGT
TTAATGTTGATAGGATATCGGCCAAAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTGTCTGAGTTAATATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAA
CTCTTGATCTGAAATGGGATGCAACAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTC
GGAGCTTCTATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAA
TGATAAAAATGCACCGATACCTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTA
ATGAGTGTAAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCT
TAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAA
TAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCC
AAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAA
AGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCA
CTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAG
AAAGATGAGAAGAA


>TRINITY_DN125594_c6_g1_i9 from sample A1 / Example unspliced isoform:
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GgtacaccttttggagcttttgcaatcagaatgttttaagattgtctggtaaaacacatcttaaatgtttatatttagatcatttgttgtctttgttgaaatctgaaagatttgtttttgatctgctgtgctgccttgtggatttactgcagTCCGATTTACAAGATCAGGTATTTGGTATTTAAACTTGTCATTGTTAT
TGTACACTGTACAGATTTATGCAGTTGTGTCATGGTACCTATTTATGTTTTTGTTAATTAACATGCAGTGTTAAGTTTTATGCCTTTTACTGTGAATGTATAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGG
GTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGA
GATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACG
AGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTA
GAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGC
GTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCG
TAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAA
GCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATC
AACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGA
TGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAAAGAAAGA
TGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAA



Intron 7 GTAATTACTC ... CTTCTCACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A118.51524128
A218.691374419
A319.831845635
A419.381313231
A515.151122416
A614.29301
A78.823160
A816.67502
A99.092514
A1012.52133



>TRINITY_DN101397_c0_g1_i2 from sample A5 / Example spliced isoform
AGGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCT
CATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATG
GGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAA
CAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATC
ACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATTTACAAGATCAGGCTC
TATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCT
GGTGAGGGT


>TRINITY_DN101397_c0_g1_i3 from sample A5 / Example unspliced isoform:
AGGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCT
CATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATG
GGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAA
CAGgtaattactctaataatttgtactttagttctctaataaatttgaagatgcattttcttcaaaaacaaagacttgaaggtttcaaatatttggataattttaaaataccgacttacgtcccttcttccataatccgtcattatgctcatgttgccctctaattgcatttttatagcttgaccttgattaaaatttgc
cttttgattgtgaatcgtcactatctgaatgtttatgttgtttaatggagctaggaagtattatagcaactattctgtaagatattgtatttgcatatattttgtcatcaacaaggtaccgatgcaattgtgaatcttatccttcaactctgctgttgatattttccttgtcttatactatatagtatcaatttcataat
aataatgaccagtgaaacgcctccctcttctcacagCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGG
GAGTGTTAAAGCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGC
ATCCTAGACAGTCCGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTG
GGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGAGGGT



Intron 8 GTTTGTATCG ... TCTTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A122.032376
A230.67521432
A330.08431819
A420.692384
A531.3423714
A626.0164342
A728.921021370
A824.441843386
A921.711101744
A1027.85571727



>TRINITY_DN118139_c2_g1_i9 from sample A3 / Example spliced isoform
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTC
ATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGG
GCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAAC
AGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCA
CCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATTTACAAGATCAGGCTCT
ATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTG
GTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGAT
ACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCG
TAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTA
AGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATG
AGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTT
CAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGC
GTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTCGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAA
GATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAA
GAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAA


>TRINITY_DN118139_c2_g1_i12 from sample A3 / Example unspliced isoform:
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTC
ATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGG
GCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAAC
AGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCA
CCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCCGATTTACAAGATCAGGCTCT
ATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTG
GTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGAT
ACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCG
TAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTA
AGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATG
AGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTT
CAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGC
GTGAAGCTGCTATGAAGGAGAAGgtttgtatcgcatccttttaaaatcatgttgcttgtttctgccatcaaatgacaagttgtgtaagttgtttttccccagtttgacgagtgctgatttcttttgcagAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAA
CCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTCGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATG
AGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAG
AAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAA



Intron 9 GTAAAGCCCC ... GTGGTGTTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A167.3930062
A267.9633270
A362.7751786
A463.5331354
A574.0320157
A6100002
A777.78207
A8100005
A978.265018
A1081.253113



>TRINITY_DN108439_c0_g2_i7 from sample A4 / Example spliced isoform
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGG
TCAACAGGCATATGGACATCAACAAGCATATGGGCAACAGGTGAGACTGTTTGTGCTTATTTAAGCATCCTTGAATTCTTAATTTTTTGGGAACAAATTTTTTGGATAACACTCAGCATACCAACATGAATGGGGCTCTTTTCAATTCTTGAAGATTTGCAAACTCTTGCCTGTGCAAATTATTTATTGCAACTGATAAA
TGTTTGAACAAATTTAAAATTTATATTGTCAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGA
CCTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAA
AGCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGAC
AGTCCGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTA
GGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGAT
AGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTC
CTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGG
CTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTC
GAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTC
TAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTA
GAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATAC
TGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGA
AGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAGAAAGCAGAGAAAAAGAAAGATGAGAAGATCACTGA


>TRINITY_DN108439_c0_g2_i1 from sample A4 / Example unspliced isoform:
GGGGCTTCCGGCCACGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCgtaaagccccacctcacaatctttccttttttattatcccctatatatatacacattcaattaacgtattctgtaattcgatggatgccacacaccgctcctc
atccttctacatttcttctcagcggttttaggcattctaacagcttagtactttgtggtgttagCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGTTCTTATCTTCCACTCGCTCATTCATTCTGTGTTAAGGGGTTCAGGGATTTTAAGGGTTTTGTTCCTGTT
AAGAGTATAAAGTCAAGGTTCGAGACCTGCTTCTCGCGTTTAGTTTAGTTAATCGTTTGCATGGGTTTGGAATTCTTACTCATTCGTTTGCAGTCAAAGAGTATCTGATGATAAGATATTTTTAGGGTTTGGGCACTCGATTAGGGTTTTTGGTGTTGTTGGCTGTTTAAAGATTTTGAGGACTTTTGCTCGCCAGGTTA
TTGGAATCGTTTCTAGGGTTATGGCTTCGTCGTTGGTTGATTGAGGCTCTAGTATGCTACAGCTTGCATTTGAACTTTTTGGGTTTGTGGGTCCTCAAAATGATGCCTTCTGGATGGTTACTAAAAAGGCCCGAGCGGCTTGTCCCTGGATTTGAAGGTTACCAAATAACCCAAACGGCATATCTCTGGATTTCATGGTT
ACCAAAAAACCCAAGCGGCATATCCAAGATTTAGGCATTACTTAAAAACCCAAGCGGCATATCTCTGGTCGAGGGTTTCATTCCATGCCAATATAGTTATCTATCTCATCACACTCCTATATAGTTATCTATCTGATCACACTCCCTGACAAAAGGTTTTTACCATTTGGGTTTAATGAATGCGAGCAGTTACAGTTTTG
ATATGAACTTGCTCATGGAATATGCATAGAGACTATCGTGCTCA



Intron 10 GTATTTGGTA ... GAATGTATAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A119.55251961
A219.05170140
A321.15261370
A420.111477.737
A515.251002.718
A633.33804
A725.3553018
A87.893503
A913.115318
A1029.2970029



>TRINITY_DN125594_c6_g1_i8 from sample A1 / Example spliced isoform
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GTCCGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAG
GACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATA
GATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCC
TGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGC
TCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTTACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGT
TTAATGTTGATAGGATATCGGCCAAAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTGTCTGAGTTAATATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAA
CTCTTGATCTGAAATGGGATGCAACAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTC
GGAGCTTCTATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAA
TGATAAAAATGCACCGATACCTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTA
ATGAGTGTAAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCT
TAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAA
TAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCC
AAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAA
AGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCA
CTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAG
AAAGATGAGAAGAA


>TRINITY_DN125594_c6_g1_i9 from sample A1 / Example unspliced isoform:
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GGTACACCTTTTGGAGCTTTTGCAATCAGAATGTTTTAAGATTGTCTGGTAAAACACATCTTAAATGTTTATATTTAGATCATTTGTTGTCTTTGTTGAAATCTGAAAGATTTGTTTTTGATCTGCTGTGCTGCCTTGTGGATTTACTGCAGTCCGATTTACAAGATCAGgtatttggtatttaaacttgtcattgttat
tgtacactgtacagatttatgcagttgtgtcatggtacctatttatgtttttgttaattaacatgcagtgttaagttttatgccttttactgtgaatgtatagGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGG
GTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGA
GATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACG
AGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTA
GAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGC
GTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCG
TAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAA
GCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATC
AACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGA
TGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAAAGAAAGA
TGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAA



Intron 11 GTGATTTGTC ... ATATTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A14.1992.324
A21.9351.301
A310.7158.507
A41.755701
A54.5542.512
A60300
A7033.800
A8018.500
A9028.800
A1001700



>TRINITY_DN125594_c6_g1_i9 from sample A1 / Example spliced isoform
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GGTACACCTTTTGGAGCTTTTGCAATCAGAATGTTTTAAGATTGTCTGGTAAAACACATCTTAAATGTTTATATTTAGATCATTTGTTGTCTTTGTTGAAATCTGAAAGATTTGTTTTTGATCTGCTGTGCTGCCTTGTGGATTTACTGCAGTCCGATTTACAAGATCAGGTATTTGGTATTTAAACTTGTCATTGTTAT
TGTACACTGTACAGATTTATGCAGTTGTGTCATGGTACCTATTTATGTTTTTGTTAATTAACATGCAGTGTTAAGTTTTATGCCTTTTACTGTGAATGTATAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGG
GTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGA
GATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACG
AGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTA
GAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGC
GTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCG
TAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAA
GCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATC
AACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGA
TGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAAAGAAAGA
TGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAA


>TRINITY_DN125594_c6_g1_i10 from sample A1 / Example unspliced isoform:
ATGTAGCTCCAGAGTTTTCTAAGgtgatttgtctgcagattagtttgatgtatttgtcatttgggaagattcatttttatttcattccgatttcttctattgatggcttaaaatatatggcatatttgcagCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGGTATTTTGTTTTT
ACCCTTTTGTATTTTAAGATGCTAGAATTTTGTAGTAAATTTTGTTATTAGTTGCAATAATTTAGATATATTAATGTATCTGGGATATGCTGCTGCCATGTTGGTTTAATGTTGATAGGATATCGGCCAAAATTCTCATGGAGCTGTGAGAACCCAAGTGGCCTATAGAGTTGTGTTTTGTTTTTATATGCTTTACTTTG
TCTGAGTTAATATTGAAACTGTTAGGTCTTTTAAAGCCTCACTAAATGGTTTTTAAAACTTGCACATCGTAAAATTGTGAAAACTGATTGAATGACTGTTATAAACTCTTGATCTGAAATGGGATGCAACAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACA
AAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTCGGAGCTTCTATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCC
CCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAATGATAAAAATGCACCGATACCTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTC
TGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTAATGAGTGTAAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAA
TGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAAT
CTGCTCTAATCCGTACAGCTATAAG



Intron 12 GTATTTTGTT ... GATGCAACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A142.227817.557
A222.644110.812
A333.333219.716
A432.55413.526
A526.093410.612
A633.3382.64
A722.58245.27
A827.59214.58
A930.43322.614
A1012.5281.74



>TRINITY_DN125594_c6_g1_i9 from sample A1 / Example spliced isoform
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GGTACACCTTTTGGAGCTTTTGCAATCAGAATGTTTTAAGATTGTCTGGTAAAACACATCTTAAATGTTTATATTTAGATCATTTGTTGTCTTTGTTGAAATCTGAAAGATTTGTTTTTGATCTGCTGTGCTGCCTTGTGGATTTACTGCAGTCCGATTTACAAGATCAGGTATTTGGTATTTAAACTTGTCATTGTTAT
TGTACACTGTACAGATTTATGCAGTTGTGTCATGGTACCTATTTATGTTTTTGTTAATTAACATGCAGTGTTAAGTTTTATGCCTTTTACTGTGAATGTATAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGG
GTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGA
GATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACG
AGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTA
GAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGC
GTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCG
TAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAA
GCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATC
AACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGA
TGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAAAGAAAGA
TGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAA


>TRINITY_DN125594_c6_g1_i8 from sample A1 / Example unspliced isoform:
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GTCCGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAG
GACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATA
GATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCC
TGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGC
TCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGgtattttgtttttacccttttgtattttaagatgctagaattttgtagtaaattttgttattagttgcaataatttagatatattaatgtatctgggatatgctgctgccatgttggt
ttaatgttgataggatatcggccaaaattctcatggagctgtgagaacccaagtggcctatagagttgtgttttgtttttatatgctttactttgtctgagttaatattgaaactgttaggtcttttaaagcctcactaaatggtttttaaaacttgcacatcgtaaaattgtgaaaactgattgaatgactgttataaa
ctcttgatctgaaatgggatgcaacagCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTAATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTC
GGAGCTTCTATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATTTACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAA
TGATAAAAATGCACCGATACCTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATAGGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTA
ATGAGTGTAAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCT
TAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAA
TAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCC
AAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAA
AGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCA
CTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAG
AAAGATGAGAAGAA



Intron 13 GTAATCTGTC ... TGTTTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A127.41944130
A232.57591443
A332.47521931
A430.654820.422
A525.6429146
A624.321427
A725.71651332
A827.1451533
A925.89731239
A1029.240429



>TRINITY_DN120287_c9_g1_i3 from sample A2 / Example spliced isoform
TACTCACTGCTTACAACTCACTAGTCACTGCTTACAACTGCTTACAACTCACTACTCACTGCTTACAACTCACTAGTCACTGCTTACAACTGCTTACAACTCACTACTCACTGCTTACAACTCACTACTCACAGCTATCAACCAACTCACACTCAACCAATGCAACCAACTCACAGCTCTCAACCACTTGAGCTAACCCA
CCAGGCTCTTTTCTTTTTATATGCCAGTCTTATAATGTAGTTATGTAAAATATGCAAAATTTGTGTCATAATAATCTGCTATTTTAAAAAATTCCCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGGGCAACAGGTGAGACTGTTTGTGCCTATTTAA
GCATCCTTGAATTCTTAATTTTTTGGGAACAAATTTTTTGGATAACACTCAGCATACCAACATGAATGGGGCTCTTTTCAATTCTTGAAGATTTGCAAACTCTTGCCTGTGCAAATTATTTATTGCAACTGATAAATGTTTGAACAAATTTAAAATTTATATTGTCAGGCTGCAACTGCATATGCTGGCAATGCATCAGC
TAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTA
CTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGT
GGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCTGATTTACAAGATCAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACT
GCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGT
ATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGAT
TTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCC
TAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTA
CACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGT
TCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCG
CTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCG
ATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAA
AAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGA
CAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAATTAAGAAAACAGAGAAGAAGAAAGATGAGAAG


>TRINITY_DN120287_c9_g1_i8 from sample A2 / Example unspliced isoform:
CGACGAGAATTGTTACCCTCAAAATCCCCATGGAGGGGCACTAACCCTAGAGCAAACGCACCGATTTTTTTTAATCCAAGACCGTAAAGCCCCACCTCACAATCTTTCCTTTTTTATTATCCCCTATATATATACACATTCAATTAACGTATTCTGTAATTCGATGGATGCCACACACCGCTCCTCATCCTTCTACATTT
CTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGCATATGGACATCAACAAGCATATGGGCAACAGGCTGCAA
CTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGACCTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCA
AGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAAGCTAATGATTATGGTTCCTTATCACCACGGGGATATAG
TCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACAGTCTGATTTACAAGATCAGGCTCTATTGATTCGACAGC
AACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGGGTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGG
GGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGAGATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCA
TGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACGAGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCAT
TTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTAGAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAAC
TGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGCGTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGgtaatctgtcgagatgaggcgacttttcggtgttg
catcaacaaacgtcttcggagcttctatgatcattttggaccatagaaagtatatttttagaattttaggtggagtttattgcgtgatccagtttattgcgtgatcctccttcagaggtttgagggccccccttcagtttatcattatcgaattactgttcgtgatgacagcggctgcgccatttactcttttagaagca
ccacatagtattcatatacaccatgtttatataatgataaaaatgcaccgataccttgctgttttgctgttggtggtgggcgcggttgttggcgaaagcatgcgaagaagaacgttccaaagtttattctgctctttaagtttcgttagaaggagacacgcttgtgtgataaaagtgttatataggattatcatcttccg
agtgatttgatctaattgattagtcttttttctaatgagtgtaaagtacaagcaaatctaaccttgccgtaaataaatttatggtgtcatttgtactggttgatttattttgtactctcttacatgtaatgttttgcagGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTT
CAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGC
TGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGA
TAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAA
AGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGAC
AAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAATAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAATTAAGAAAACAGAGAAGAAGAAAGATGAGAAG



Intron 14 GTTAGTGGTA ... GTTGTTTCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A119.83952621
A215.79641410
A311.2859123
A416.54531110
A518.522246
A610.531713
A710.484747
A810.8166511
A99.6870411
A106.023914



>TRINITY_DN125594_c6_g1_i9 from sample A1 / Example spliced isoform
GATGGATGCCACACACCGCTCCTCATCCTTCTACATTTCTTCTCAGCGGTTTTAGGCATTCTAACAGCTTAGTACTTTGTGGTGTTAGCGAAGAAGAATACGAAGGAGATGGATCAGTGGAACTATCCGAGGTCCGCTCAACAGTCCGGGCAGGGATACTCTCCAGCAATATCAGCACAACAATCAACAGGTCAACAGGC
ATATGGACATCAACAAGCATATGGGCAACAGGCTGCAACTGCATATGCTGGCAATGCATCAGCTAGCTCGGGTGGAGTATCACACCCATCCCAGCGTGCCCCTTTGAGTACATCTGCAGGGGCATCAGCTGCTACAGGTGGTAGTGGAACATCAGCAGGGGCACTTCAGTATGGTGGGCAATATGGTTCTATTTATGGAC
CTTCAACTCAAAGTGGTGCTCAACAGCCCACAGGAGCAAGTGGGAAGGGTGGGTCTGCTTCTACTTTTCAGGGATATGGTGCTCGAGCTGGTGGATCATTTGGTGGGGGTGCATCAGGAGTTAATGCCTCAGCTGCCTATGGGCTAGAGTCTTCAAAGTATATATCTACTGGTTCAGGCACAGGTTTTGGGAGTGTTAAA
GCTAATGATTATGGTTCCTTATCACCACGGGGATATAGTCAGAAAGGTGATCAGTATGCTGGTGGGTATGCTACGAAGGCTTCTGATTATGCATCTTCAGATAGGGGCCAGTATGGTCTAAATCAGAGTGCTTACCTTGGTAGAGACGTTCAAAGTGATGCAGCCAGGCGGTATCCGGAGGGTGTTGGGCATCCTAGACA
GGTACACCTTTTGGAGCTTTTGCAATCAGAATGTTTTAAGATTGTCTGGTAAAACACATCTTAAATGTTTATATTTAGATCATTTGTTGTCTTTGTTGAAATCTGAAAGATTTGTTTTTGATCTGCTGTGCTGCCTTGTGGATTTACTGCAGTCCGATTTACAAGATCAGGTATTTGGTATTTAAACTTGTCATTGTTAT
TGTACACTGTACAGATTTATGCAGTTGTGTCATGGTACCTATTTATGTTTTTGTTAATTAACATGCAGTGTTAAGTTTTATGCCTTTTACTGTGAATGTATAGGCTCTATTGATTCGACAGCAACATTTACTGCAAGCACAGCAACTGCAATCTGTTGCTGCATTGAGACAAGGCGCCACTGCTACAGCTCTAGATGCGG
GTGGAAGGGCTGCAGATTATTTACCTGGAAGGGGAAGTGCAATGAACCCTCCGTCAAGGCTGGATGCAGATGTGGGCTTGCTAGGACCTCGTGGTGCAGGCCCTGCTGGTGGGGGTACTGGGGGGAGAGGGCTTGAAGATCTAGTGTATGGGCAGGGGGAGCATCATGCAGTCTATGGTGTCAGCTTGCCACCTGGCCGA
GATTATAGCTCCGGTAAAGGCCCTGTTCCTGTTGGTGGTGCTTCTTGGGATGCAGGCTATGGTGGCAGTGTTGGAAGTCGGATAGATCGTTTTGAGGATCAGCGAGATACTTTGCTTCGGCATGAGTATGATAGGAGAGAGGAGGATTTGCGAAAAGAGCGCGAAAGGGAGAGGGAGCGAGAGCGTGAATTGGAGAGACG
AGAGCGTGAATTGGAGCGACGAGAGCGTGAGCGGGAACGTGATCGTCAACGGGAACGTGAACTTCGGAGACGAGATCGTACTCCTGTTAGACCTCTTCCTAGGGTTCGTAGAGAACCTTCATTTTCCAGGAAAATTGAGAGGGTTCCTAGGAGAGTTTCTCCTCGCCGTGAGCCCATTCGCAGGCGCGTATCCCCGGTTA
GAGAGTACGTCTGCAAGATTGAGCCTCACACTCTTGTTGAAGTTGAAAGGGATTTCCCATCTGTTAGCAAACGCTACTCAAGGCTCTATGTAGCTCCAGAGTTTTCTAAGCTTGTTGTGAACTGGTTAAAGGAAGACCTTGATATTACACTAACAACCCCTGTCAGCTTTGTGCATGATGCTGTAGTAGAGGATGAGAGC
GTTCCGAAGGAGTCTTCATCAAAGTTGTCTGACAAAGTAGCAGGAGGCATGATACTGGAGGAGGAAGGCACGATTTGGAATTCGAAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTGGTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCG
TAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAA
GCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGCCTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATC
AACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAAAGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGA
TGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATGAGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGATCATTGAAGACAAGAAAGCAGAGAAGAAGAAAGATGAGAAGATCACTGAAGACAAAAGAAAGA
TGAGAAGATCACTGAAGTTAAGAAAACAGAGAAGAAGAAAGATGAGAAGATCACTGAAGATAAGAAATCAGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAAGGATACTGACAAGAAGAAAGACGAGAAGAAGAAAGATGAGAAGAA


>TRINITY_DN125594_c6_g1_i6 from sample A1 / Example unspliced isoform:
AATCTGTCGAGATGAGGCGACTTTTCGGTGTTGCATCAACAAACGTCTTCGGAGCTTCTATGATCATTTTGGACCATAGAAAGTATATTTTTAGAATTTTAGGTGGAGTTTATTGCGTGATCCTCCTTCAGAGGTTTGAGGGCCCCCCTTCAGTTTATCATTATCGAATTACTGTTCGTGATGACAGCGGCTGCGCCATT
TACTCTTTTAGAAGCACCACATAGTATTCATATACACCATGTTTATATAATGATAAAAATGCACCGATACCTTGCTGTTTTGCTGTTGGTGGTGGGCGCGGTTGTTGGCGAAAGCATGCGAAGAAGAACGTTCCAAAGTTTATTCTGCTCTTTAAGTTTCGTTAGAAGGAGACACGCTTGTGTGATAAAAGTGTTATATA
GGATTATCATCTTCCGAGTGATTTGATCTAATTGATTAGTCTTTTTTCTAATGAGTGTAAAGTACAAGCAAATCTAACCTTGCCGTAAATAAATTTATGGTGTCATTTGTACTGGTTGATTTATTTTGTACTCTCTTACATGTAATGTTTTGCAGGTCATTCTAATGAGTGGAATGAGTGAAAATGTTATAGCAGAGTTG
GTTTCTGATAAAGGTTCAGCGGATAGAGGCCTACACATGCATAATGCTCTTAGGTTTTCTGTTCTTCGTAAAGAGCACACATTTTTATTAGCTGGAGGGGCATGGGACAGGGTTTTAGATGGTGGTGACCCAGCTACAGATCAATCTGCTCTAATCCGTACAGCTATAAGgttagtggtaaattggtttcttagtatttg
ttgagacttttaactgctgtagcagacctcatacaattagtttctgttgtttcagATGCTTCAAGGAAGTCACACAACTTGATCTATCAAGCTGCAAGCGCTGGAGTCGTTTTGTTGAGGTGCACTATGACAGAATAGGTAAAGGCAGTCCAAGCCACAGAGAAATCACTGTTATTTTTATTCCGGACTTATCTACATGC
CTGCCTTCTTTGGAGAGCTGGAAGGTCCAGTATTTAGAGCATAAAAAAGCTGAGAGAGAGCGTGAAGCTGCTATGAAGGAGAAGAAAGGAAAGGCCAGCGATAAGAAGGATGTTATTAAAGGGAACTCGGGCTCCAAAGAAGAAAAGGAGATCAACCCGGCAGATAAAGCTAAGAAAGAAAAGGAAGACAAGAAAGATAA
AGATAAAAACTTGAAGGATGAGAAAAAGGAGAATACTGATAACAAGGAAATAGAAAAGAAAGATGAAAACAAAGAGAAGGAAGGAAAAAAAGCAGAGAAAAAGAAGGATGAAAAGAAGGTGACCGAGGAAAAGAAAGCAGAAAAAAAGAAAGATGAGAAGAAGGTGACCGAGGAAAAAAAACCAGAGAAGAAGAAAGATG
AGAAGGACATTGAGGAAAAGAAATCAGTGAAGAAGAAGGATCAGAAGGACGTGGAGGAAAAGAAGGTAGAGAAGAAGAAAGATGAGAAGA