NA ----> NA

Intron 1 GTTAGTGTGT ... TTTATGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.06	22.3	3	6
A2	42.27	12.3	9	4
A3	31.64	28.7	13	3
A4	31.11	18.7	8	1
A5	43.01	9.3	7	1
A6	29.04	70	27	1
A7	28.76	55	20	0
A8	34.15	36.7	18	1
A9	28.87	55.3	20	1
A10	29.76	52	20	0

>TRINITY_DN108431_c0_g1_i8 from sample A3 / Example spliced isoform

ATCACCTTGAAAAAAGCAATGGTAAAACTCCATATAAAATACAATGCCAAATGCAAAGATATGAACAATGCACTGCATCAGATCGAGTGGCACTGAGTTATAACGTCTCTGAGCACTCGCTAGAGATTGCCAATTATAACAGGTGTTAAGCAGTAGCATTAGAGAGTTCTGTCTGCTTCTTTATTTGCAATATAACAGTG
CGAGCGAAAGGATGAACGGAGCAAGGAGGCGTGTGGCTCAACAAATATGGAGCGCCATTCACATTCATTCTTCAAGCAAAACCGGAAGTTCCCTCTCTCCCCAGCTTCTCGATCTTCCCTGCCTCGCTCTTCAACAACGAGGAGTCAAAGTAAATGGATCAGATGTTAAACATGGGAACATCATTGAGAAAAATGGGCGT
CTTCATGAGGTAATAATCTTCCACCAGTCTACTAGCTACTATGGGAAGCAAAACTATAGGTCCCAAAAAAGAATCGTGTCAGAACTAAAATGTTATAAAGGCACAACACACTCAACATGGACGAGGAGGAGCAACGATACAGGTTGAACTCCGTGATGTGGAGAATGGGCTCAAGTCAACTGAGAGATTGCGCACATCTG
AATCTATTGAACGAGTCCATGTTGAAGACAAGTCTTTTCAATTTTTGTACGAGGATGGTGATATCATATTTGTAATGGACCCTAAAACCTTTGAGCAAGTAGAGCTTTCAAAGGAAGTTTTTGGCAAACGAGCTTCATATTTGACAGATGGCATGCAAGTTACTGTGAGCTTTCATGAAGGAAGAGCATTGTCTGCATCC
GTGCCAACTCGTGTAACTTGCACAGTTGTTCAAGCGGAACCTTCCTTTAAAGGCCAATCTGCCTCTGCTCAGTATAAAAAGCTTATCCTAGAAAATGGACGAACAGTGATGGGACCTACTTATTTAGAAGTTGGTGATAAGATAGTTGTGAATACACTTGATGAATCCTATATGGCTAGGGCTTCCAAAAGTTAGCAGAG
CAACTTTGCAACTGGGAAGACTTTTATTTTGTGGATTCCATCTGCTTGAGATTTTGAATTTATTGCGGATTTTATGTATAATAAGATGTTATGGCGTTGGTCCTATTAATAAACATTAGAATTGGCTGCATTACACATTGGTTTTGCAGGCATTTAGAGAAATTTATAGTAGACTATATAGAATACAAATTTAACTAAAA
TCATATGCAAACCTCACGAGTTCCTATACTTCAATTTGTATTTTATATACAAAGCTCTCATCAATGAAAGCATGTCCCAAATTTTGGGACAATTTTATTATGCAGGGCATATCTATGTCTTACAGTTACTAACGAATATATTTTTTGCCAAAAGATTGACTGTGT

>TRINITY_DN108431_c0_g1_i3 from sample A3 / Example unspliced isoform:

ATCACCTTGAAAAAAGCAATGGTAAAACTCCATATAAAATACAATGCCAAATGCAAAGATATGAACAATGCACTGCATCAGATCGAGTGGCACTGAGTTATAACGTCTCTGAGCACTCGCTAGAGATTGCCAATTATAACAGGTGTTAAGCAGTAGCATTAGAGAGTTCTGTCTGCTTCTTTATTTGCAATATAACAGTG
CGAGCGAAAGGATGAACGGAGCAAGGAGGCGTGTGGCTCAACAAATATGGAGCGCCATTCACATTCATTCTTCAAGCAAAACCGGAAGTTCCCTCTCTCCCCAGCTTCTCGATCTTCCCTGCCTCGCTCTTCAACAACGAGGAGTCAAAGTAAATGGATCAGATGTTAAACATGGGAACATCATTGAGAAAAATGgttag
tgtgttaacttaaataccatatcttggtacctgaaagtacaatttttgttgagtaatatattcgttcattcatttaacgtatctagtttggatagtgtttgaggttatttagtttcttcactgattctacttgaaattgtttatgacagGGCGTCTTCATGAGGTAATAATCTTCCACCAGTCTACTAGCTACTATGGGA
AGCAAAACTATAGGTCCCAAAAAAGAATCGTGTCAGAACTAAAATGTTATAAAGGCACAACACACTCAACATGGACGAGGAGGAGCAACGATACAGGTTGAACTCCGTGATGTGGAGAATGGGCTCAAGTCAACTGAGAGATTGCGCACATCTGAATCTATTGAACGAGTCCATGTTGAAGACAAGTCTTTTCAATTTTT
GTACGAGGATGGTGATATCATATTTGTAATGGACCCTAAAACCTTTGAGCAAGTAGAGCTTTCAAAGGAAGTTTTTGGCAAACGAGCTTCATATTTGACAGATGGCATGCAAGTTACTGTGAGCTTTCATGAAGGAAGAGCATTGTCTGCATCCGTGCCAACTCGTGTAACTTGCACAGTTGTTCAAGCGGAACCTTCCT
TTAAAGGCCAATCTGCCTCTGCTCAGTATAAAAAGCTTATCCTAGAAAATGGACGAACAGTGATGGGACCTACTTATTTAGAAGTTGGTGATAAGATAGTTGTGAATACACTTGATGAATCCTATATGGCTAGGGCTTCCAAAAGTTAGCAGAGCAACTTTGCAACTGGGAAGACTTTTATTTTGTGGATTCCATCTGCT
TGAGATTTTGAATTTATTGCGGATTTTATGTATAATAAGATGTTATGGCGTTGGTCCTATTAATAAACATTAGAATTGGCTGCATTACACATTGGTTTTGCAGGCATTTAGAGAAATTTATAGTAGACTATATAGAATACAAATTTAACTAAAATCATATGCAAACCTCACGAGTTCCTATACTTCAATTTGTATTTTAT
ATACAAAGCTCTCATCAATGAAAGCATGTCCCAAATTTTGGGACAATTTTATTATGCAGGGCATATCTATGTCTTACAGTTACTAACGAATATATTTTTTGCCAAAAGATTGACTGTGT

Intron 2 GTATGTTAAT ... TGGTTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	2	2	6
A2	100	0	2	4
A3	100	0	2	2
A4	75	2	4	8
A5	20	2	0	1
A6	8.33	11	0	2
A7	28.57	5	0	4
A8	33.33	4	2	2
A9	0	5	0	0
A10	0	18	0	0

>TRINITY_DN98774_c0_g1_i8 from sample A4 / Example spliced isoform

ATGGGAAGCAAAACTATAGGTCCCAAAAAAGAATCGTGTCAGAACTAAAATGTTATAAAGGCACAACACACTCAACATGGACGAGGAGGAGCAACGATACAGGTTGAACTCCGTGATGTGGAGAATGGGCTCAAGTCAACTGAGAGATTGCGCACATCTGAATCTATTGAACGAGTCCATGTTGAAGACAAGTCTTTTCA
ATTTTTGTACGAGGATGGTGATATCATATTTGTAATGGACCCTAAAACCTTTGAGCAAGTAGAGCTTTCAAAGGAAGTTTTTGGCAAACGAGCTTCATATTTGACAGGTATAAAAAGCTTATCCTAGAAAATGGACGAACAGTGATGATACATTACAACATGAACTTGTCATCAAAAGTTTGTCATTCGGCATTGATGGT
TTGTGGATACATTACGAGATAAATTTGTCATAAAAAGTTCATTATTTGACATCAACAGTCCATGGATACATTACAAGATGAGTTTATTATCAACATCATTTGGCATTGATGGTTCATGAGTGCTTTTGCTAGACTTAGC

>TRINITY_DN98774_c0_g1_i1 from sample A4 / Example unspliced isoform:

GTATAAAAAGCTTATCCTAGAAAATGGACGAACAGTGATGgtatgttaataggctattatttatgacctttagtttatttggtttttctttttccttttgcatcttgatcaggctataaattttttggtaagggtaaatgcttttttaaatccatggggagttgtaaatcccaacgccacattatcctggtaaagtccac
tttaatgtgggagtcatcaaacacaagtaactcatacagattgttatcatttagaatgggcttttcaaaaagttcgtattgattggttcaacattgatggttcacagATACATTACAACATGAACTTGTCATCAAAAGTTTGTCATTCGGCATTGATGGTTTGTGGATACATTACGAGATAAATTTGTCATAAAAAGTTC
ATTATTTGACATCAACAGTCCATGGATACATTACAAGATGAGTTTATTATCAACATCATTTGGCATTGATGGTTCATGAGTGCTTTTGCTAGACTTAGC

Intron 3 GTATAAAAAG ... ATATGGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.65	9	25	28
A2	78.95	4	11	19
A3	71.83	10	25	26
A4	63.64	6	10	11
A5	64.71	3	4	7
A6	67.06	70	121	164
A7	67.35	48	112	86
A8	72.71	61	137	188
A9	66.32	48	85	104
A10	64.57	45	73	91

>TRINITY_DN44485_c0_g1_i1 from sample A10 / Example spliced isoform

CAACTAGGTTAATCTTGTTCACTTTAGATAGTTAGGTGGAATACCCAAATTTTATTATTGTATGCCACATGATGTTCTTGTATCATGATTTGTTCTTGTTCTTGTATCATGATTTGACTAGCCTTGAAAAAATTGGTGAATCACCTTGAAAAAAGCAATGGTAAAACTCCATATAAAATACAATGCCAAATGCAAAGATA
TGAACAATGCACTGCATCAGATCGAGTGGCACTGAGTTATAACGTCTCTGAGCACTCGCTAGAGATTGCCAATTATAACAGGTGTTAAGCAGTAGCATTAGAGAGTTCTGTCTGCTTCTTTATTTGCAATATAACAGTGCGAGCGAAAGGATGAACGGAGCAAGGAGGCGTGTGGCTCAACAAATATGGAGCGCCATTCA
CATTCATTCTTCAAGCAAAACCGGAAGTTCCCTCTCTCCCCAGCTTCTCGATCTTCCCTGCCTCGCTCTTCAACAACGAGGAGTCAAAGTAAATGGATCAGATGTTAAACATGGGAACATCATTGAGAAAAATGGGCGTCTTCATGAGGTTATAAAGGCACAACACACTCAACATGGACGAGGAGGAGCAACGATACAGG
TTGAACTCCGTGATGTGGAGAATGGGCTCAAGTCAACTGAGAGATTGCGCACATCTGAATCTATTGAACGAGTCCATGTTGAAGACAAGTCTTTTCAATTTTTGTACGAGGATGGTGATATCATATTTGTAATGGACCCTAAAACCTTTGAGCAAGTAGAGCTTTCAAAGGAAGTTTTTGGCAAACGAGCTTCATATTTG
ACAGATGGCATGCAAGTTACTGTGAGCTTTCATGAAGGAAGAGCATTGTCTGCATCCGTGCCAACTCGTGTAACTTGCACAGTTGTTCAAGCGGAACCTTCCTTTAAAGGCCAATCTGCCTCTGCTCAGGCTTCCAAAAGTTAGCAGAGCAACTTTGCAACTGGGAAGACTTTTATTTTGTGGATTCCATCTGCTTGAGA
TTTTGAATTTATTGCGGATTTTATGTATAATAAGATGTTATGGCGTTGGTCCTATTAATAAACATTAGAATTGGCTGCATTACACATTGGTTTTGCAGGCATTTAGAGAAATTTATAGTAGACTATATAGAATACAAATTTAACTAAAATCATATGCAAACCTCACGAGTTCCTATACTTCAATTTGTATTTTATATACA
AAGCTCTCATCAATGAAAGCATGTCCCAAATTTTGGGACAATTTTATTATGCAGGGCATATCTATGTCTTACAGTTACTAACGAATATATTTTTTGCCAAAAGATTGACTGTGTTATTTTTTCGAATATTATCATGATATTGCTACTATTATTGTTGGTATGAGTGAAAATTTTGGATTTAGCCTCTTTAGTTCAAAAA

>TRINITY_DN44485_c0_g1_i2 from sample A10 / Example unspliced isoform:

CAACTAGGTTAATCTTGTTCACTTTAGATAGTTAGGTGGAATACCCAAATTTTATTATTGTATGCCACATGATGTTCTTGTATCATGATTTGTTCTTGTTCTTGTATCATGATTTGACTAGCCTTGAAAAAATTGGTGAATCACCTTGAAAAAAGCAATGGTAAAACTCCATATAAAATACAATGCCAAATGCAAAGATA
TGAACAATGCACTGCATCAGATCGAGTGGCACTGAGTTATAACGTCTCTGAGCACTCGCTAGAGATTGCCAATTATAACAGGTGTTAAGCAGTAGCATTAGAGAGTTCTGTCTGCTTCTTTATTTGCAATATAACAGTGCGAGCGAAAGGATGAACGGAGCAAGGAGGCGTGTGGCTCAACAAATATGGAGCGCCATTCA
CATTCATTCTTCAAGCAAAACCGGAAGTTCCCTCTCTCCCCAGCTTCTCGATCTTCCCTGCCTCGCTCTTCAACAACGAGGAGTCAAAGTAAATGGATCAGATGTTAAACATGGGAACATCATTGAGAAAAATGGGCGTCTTCATGAGGTTATAAAGGCACAACACACTCAACATGGACGAGGAGGAGCAACGATACAGG
TTGAACTCCGTGATGTGGAGAATGGGCTCAAGTCAACTGAGAGATTGCGCACATCTGAATCTATTGAACGAGTCCATGTTGAAGACAAGTCTTTTCAATTTTTGTACGAGGATGGTGATATCATATTTGTAATGGACCCTAAAACCTTTGAGCAAGTAGAGCTTTCAAAGGAAGTTTTTGGCAAACGAGCTTCATATTTG
ACAGATGGCATGCAAGTTACTGTGAGCTTTCATGAAGGAAGAGCATTGTCTGCATCCGTGCCAACTCGTGTAACTTGCACAGTTGTTCAAGCGGAACCTTCCTTTAAAGGCCAATCTGCCTCTGCTCAgtataaaaagcttatcctagaaaatggacgaacagtgatgggacctacttatttagaagttggtgataagat
agttgtgaatacacttgatgaatcctatatggctagGGCTTCCAAAAGTTAGCAGAGCAACTTTGCAACTGGGAAGACTTTTATTTTGTGGATTCCATCTGCTTGAGATTTTGAATTTATTGCGGATTTTATGTATAATAAGATGTTATGGCGTTGGTCCTATTAATAAACATTAGAATTGGCTGCATTACACATTGGTT
TTGCAGGCATTTAGAGAAATTTATAGTAGACTATATAGAATACAAATTTAACTAAAATCATATGCAAACCTCACGAGTTCCTATACTTCAATTTGTATTTTATATACAAAGCTCTCATCAATGAAAGCATGTCCCAAATTTTGGGACAATTTTATTATGCAGGGCATATCTATGTCTTACAGTTACTAACGAATATATTT
TTTGCCAAAAGATTGACTGTGTTATTTTTTCGAATATTATCATGATATTGCTACTATTATTGTTGGTATGAGTGAAAATTTTGGATTTAGCCTCTTTAGTTCAAAAA