NA ----> NA

Intron 1 GTAACATTTG ... TGGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.02	99.3	73	501.3
A2	80.07	65.2	48	276.7
A3	82.26	83.6	46	427.3
A4	80.22	72.1	48	330.3
A5	81.27	33.4	20	161.7
A6	73.21	11.1	2	29
A7	61.1	33	6	29.7
A8	65.08	20	1	29
A9	56.11	23.6	4	21.3
A10	66.67	15.4	5	26.7

>TRINITY_DN111899_c1_g1_i4 from sample A4 / Example spliced isoform

TGATGGGTTCTAGCTTTGCAGGGAGAGCGATTCGAGGTAAAGCGCGATGTATGTTCACCGAGAGCAATTCCAGCCTTACGGGAGGAGCCGCCACTGAAGACAAAATGAAGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAGGTATGATATACACATATTTAGAT
AGAAATATTTAGATAGATGTTGTGCCAATAAATGTGCATAGAATGGGATGCCCTCTGGTAGATAATGGTTTGGACGGATATGCATACAGTTCAAACTTCAAAGGGTTGTTTCATGGTCTTTTTATGAGTTTTATCTTTTATTTCTACAGCAGTAAAATTGCAGGGTCTTAAGTCTTCAGAGAATCCTCATTGAGCTTTCA
ATACTAGAAAGTAATATGAGATAAAGCGCCATTTATACTCACCGATTCCTTCAACTTGTTTGATGGGTCAGTTGCATACCGATCCTTCAATTTAT

>TRINITY_DN111899_c1_g1_i11 from sample A4 / Example unspliced isoform:

CTCACCGATTCCTAAAATTTGTTTGATGGGTTACTTGCTTATCTACGTTTTTACTGGCTAAGAATTTTTCATTTTAAGGTTGAAACGAGATCTGATTTTACAGCCTTACGGGAGGAGCCGCCACTGAAGACAAAATGAAGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGgtaacatttgccatcctctctgctctg
ttgatggagtttcagccggtacaggtaacataaagctcttctatcttttccgctatctgtgatgggttgtagctttgccatcttctctgctctgttgatggagtttgccatgcttatctctcttttaagctataaagctatagaaatttagttttctaaggcagtgtgtttggttggtttgcagTTTAGGGACGTATGAT
ATACAAATATTTAAGCAGGAGTCGCCATTGAAGAGAAGATGAGGCAAGAAGATTACAT

Intron 2 GTATGATATA ... ATTTCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	78.08	208	151	740.5
A2	79.16	129	114	490
A3	79.63	170	171	664.5
A4	79.5	141	153	547
A5	84.97	48	70	271.5
A6	60	20	9	30
A7	40.48	86	16	58.5
A8	53.33	42	7	48
A9	41.05	56	12	39
A10	51.82	33	7	35.5

>TRINITY_DN111899_c1_g1_i14 from sample A4 / Example spliced isoform

TGATGGGTTCTAGCTTTGCAGGGAGAGCGATTCGAGGTAAAGCGCGATGTATGTTCACCGAGAGCAATTCCAGCCTTACGGGAGGAGCCGCCACTGAAGACAAAATGAAGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAGCAGTAAAATTGCAGGGTCTTAAG
TCTTCAGAGAATCCTCATTGAGCTTTCAATACTAGAAAGTAATATGAGATAAAGCGCCATTTATACTCACCGATTCCTTCAACTTGTTTGATGGG

>TRINITY_DN111899_c1_g1_i8 from sample A4 / Example unspliced isoform:

TGATGGGTTCTAGCTTTGCAGGGAGAGCGATTCGAGGTAAAGCGCGATGTATGTTCACCGAGAGCAATTCCAGCCTTACGGGAGGAGCCGCCACTGAAGACAAAATGAAGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAGgtatgatatacacatatttagat
agaaatatttagatagatgttgtgccaataaatgtgcatagaatgggatgccctctggtagataatggtttggacggatatgcatacagttcaaacttcaaagggttgtttcatggtctttttatgagttttatcttttatttctacagCAGTAAAATTGCAGGGTCTTAAGTCTTCAGAGAATCCTCATTGAGCTTTCA
ATACTAGAAAGTAATATGAGATAAAGCGCCATTTATACTCACCGATTCCTTCAACTTGTTTGATGGGAGTCGCCATTGAAGACAAGATGAGGCAAGAAGATTACATTCCTTAGCGGGTACAGGAC

Intron 3 GTATGATATA ... TCTACATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	76.78	170	469.5	670
A2	74.63	123	283.5	445
A3	83.02	103	417.5	602
A4	81.66	93	352.5	487
A5	86.55	33	188.5	241
A6	54.51	21	20.5	31
A7	31.73	74	26	43
A8	48.42	42	33	46
A9	39.8	40	19	34
A10	42.42	43	29.5	34

>TRINITY_DN113465_c1_g1_i6 from sample A5 / Example spliced isoform

TGGGGCAAAAGGATTACATTCCTACGTTTCATTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAGGAAAATTGCAGGGTCTTTAGTCTTCAGAGAAACCTCATTGAGCTTTCAATAAGAGAAAGTAATATGAGATAAAGCGCTACTTATACTCACCAATTCCTTCAATTTGTTTGATGGGTCAGT
TGCATACCGA

>TRINITY_DN113465_c1_g1_i1 from sample A5 / Example unspliced isoform:

TGGGGCAAAAGGATTACATTCCTACGTTTCATTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAGgtatgatatacgaatatttagatagatgttgcgccaataaatgtgcacagaatgggatgccttctagtagatagtggtttggatggatatgcatacagttcaaacttcaaagggttgatt
catggtctttttatgagttttatcttttatttctacatcagGAAAATTGCAGGGTCTTTAGTCTTCAGAGAAACCTCATTGAGCTTTCAATAAGAGAAAGTAATATGAGATAAAGCGCTACTTATACTCACCAATTCCTTCAATTTGTTTGATGGGTCAGTTGCATACCGA

Intron 4 GTATGATATA ... TCTACAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.37	98	666	690
A2	91.77	36	361	442
A3	87.66	85	599	609
A4	87.94	69	502	504
A5	87.2	37	262	242
A6	85.29	5	28	30
A7	90.7	4	30	48
A8	78.43	11	39	41
A9	92.98	2	22	31
A10	97.06	1	33	33

>TRINITY_DN113465_c1_g1_i9 from sample A5 / Example spliced isoform

ACAGGTAACATAAAGCTCTTCTATCTTTGCCATCCTCTCTGCTCTGTTGATGGAGTTTCAGCCGGTACAGGTAACATAAAGCTCTTCTAAGGCAGCAGTGTTGATGGAGTTTGCCGTGCTTATCTCTCTTTCAAGTTATAGAAATTTAGTTTTCTAAGGCAGTGTGTTTGGTTGGTGTGCAGTTTAGGGACGTATGATAT
ACAAATATTTAAGCAGGAAAATTGCAGGGTCTTTAGTCTTCAGAGAAACCTCATTGAGCTTTCAATA

>TRINITY_DN113465_c1_g1_i11 from sample A5 / Example unspliced isoform:

ACAGGTAACATAAAGCTCTTCTATCTTTGCCATCCTCTCTGCTCTGTTGATGGAGTTTCAGCCGGTACAGGTAACATAAAGCTCTTCTAAGGCAGCAGTGTTGATGGAGTTTGCCGTGCTTATCTCTCTTTCAAGTTATAGAAATTTAGTTTTCTAAGGCAGTGTGTTTGGTTGGTGTGCAGTTTAGGGACGTATGATAT
ACAAATATTTAAGCAGgtatgatatacaaatatttagatagatgttgtgccaataaatgtgcatagaatgggatgccctctggtagataatggtttggacggatatgcatacagttcaaacttcaaagggttgtttcatggtctttttatgagttttatcttttatttctacagcagGAAAATTGCAGGGTCTTAAGTCT
TCAGAGAAACCTCATTGAGCTTTCAATAAGAGAAAGTAATATGAGATAAACCGCCATTTATACTTACCAATTCCTTCAATTTGTTTTGACCGGTTAAGAATTTTGACCGGTTGAAATGAGATCTGATTTTACAGCCTTCCAGGAGGAGTCGCCATTGA

Intron 5 GTATGATATA ... ATTTCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.89	208	513.3	737
A2	75.19	129	296	488.7
A3	76.65	170	458.7	664.7
A4	76.46	141	380.7	544
A5	82.92	48	198	270.7
A6	56.42	20	22	30
A7	32.72	86	26.7	57
A8	48.36	42	31.7	47
A9	34.5	56	20.7	38.3
A10	51.55	33	33	37.3

>TRINITY_DN111899_c1_g1_i14 from sample A4 / Example spliced isoform

TGATGGGTTCTAGCTTTGCAGGGAGAGCGATTCGAGGTAAAGCGCGATGTATGTTCACCGAGAGCAATTCCAGCCTTACGGGAGGAGCCGCCACTGAAGACAAAATGAAGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAGCAGTAAAATTGCAGGGTCTTAAG
TCTTCAGAGAATCCTCATTGAGCTTTCAATACTAGAAAGTAATATGAGATAAAGCGCCATTTATACTCACCGATTCCTTCAACTTGTTTGATGGG

>TRINITY_DN111899_c1_g1_i2 from sample A4 / Example unspliced isoform:

TGTTGATGGAGTTTGCCGTGCTTATCTCTCTTTCAAGTTATAGAAATTTAGTTTTCTAAGGCAGTGTGTTTGGTTGGTGTGCAGTTTAGGGACGTATGATATACAAATATTTAAGCAGgtatgatatacacatatttagatagatgtgccaataaatgtgcacagaatgggatgccctggcgtagataatggtttgcatg
gatatgcatacagttcatacttcaaagggttgtttcatggtctttttatgagttttatcttttatttctacagCAGTAAAATTGCAGGGTCTTAAGTCTTCAGAGAATCCTCATTGAGCTTTCAATACTAGAAAGTAATATGAGATAAAGCGCCATTTATACTCACCGATTCCTTCAACTTGTTTGATGGGAGTCGCCAT
TGAAGACAAGATGAGGCAAGAAGATTACATTCCTTAGCGGGTACAGGAC

Intron 6 GTAACATAAA ... TGGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	92.72	17	175	258
A2	93.69	10	139	158
A3	96.22	8	179	228
A4	87.43	21	129	163
A5	95.7	4	89	89
A6	83.78	3	14	17
A7	84.31	4	20	23
A8	62.26	10	10	23
A9	48.57	9	3	14
A10	65.96	8	17	14

>TRINITY_DN111899_c1_g1_i9 from sample A4 / Example spliced isoform

TGTTGATGGAGTTTGCCGTGCTTATCTCTCTTTCAAGTTATAGAAATTTAGTTTTCTAAGGCAGTGTGTTTGATTGTTGTGCAGTTTAGGGACGTATGGTTTAGGGACGTATGATATACAAATATTTAAGCAGGTATGATATACACATATTTAGATAGAAATATTTAGATAGATGTTGTGCCAATAAATGTGCACAGAAT
GGGATGCCCTCTGGTAGATAATGGTTTGGATGGATATGCATACAGTTCAAACTTCAAAGGGTTGGGTTGTTTCATGGTCTTTTTATGAGTTTTATCTTTTATTTCTACAGCAGGAAAATTGCAGGGTCTTAAGTCTTCAGAGAATCCTCATTGAGCTTTCAATACTAGAAAGTAATATGAGATAAAGCGCCATTTATACT
CACCGATTCCTTCAACTTGTTTGATGGGAGTCGCCATTGAAGACAAGATGAGGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGTTTAGGGACGTATGATATACAAATATTTAAGCAG

>TRINITY_DN111899_c1_g1_i11 from sample A4 / Example unspliced isoform:

CTCACCGATTCCTAAAATTTGTTTGATGGGTTACTTGCTTATCTACGTTTTTACTGGCTAAGAATTTTTCATTTTAAGGTTGAAACGAGATCTGATTTTACAGCCTTACGGGAGGAGCCGCCACTGAAGACAAAATGAAGCAAGAAGATTACATTTCGTTTCAGCCGGTACAGGTAACATTTGCCATCCTCTCTGCTCTG
TTGATGGAGTTTCAGCCGGTACAGgtaacataaagctcttctatcttttccgctatctgtgatgggttgtagctttgccatcttctctgctctgttgatggagtttgccatgcttatctctcttttaagctataaagctatagaaatttagttttctaaggcagtgtgtttggttggtttgcagTTTAGGGACGTATGAT
ATACAAATATTTAAGCAGGAGTCGCCATTGAAGAGAAGATGAGGCAAGAAGATTACAT