sp|Q8W468|ARI8_ARATH ----> Probable E3 ubiquitin-protein ligase ARI8

Intron 1 GTTTGGTTTT ... TTGAGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.31	27.5	5.4	3.6
A2	1.08	33	0.4	1.8
A3	23.8	13.9	4.4	4.8
A4	20.21	23.6	6.7	5.5
A5	50	6	6	4
A6	7.79	27.9	2.4	4.6
A7	14.4	35.7	6.2	5.7
A8	8.43	35.7	3.6	4.5
A9	10.42	28.8	3.5	2.6
A10	1.02	34.9	0.4	3.3

>TRINITY_DN124892_c0_g2_i5 from sample A1 / Example spliced isoform

TTGACTACTATACAGGGATGATATGAGTGAAAACATGATTTGAGCTTCAAATCACAAATGCAAATAACTGTTCATGCCTTTTGAACTAAAGGTGTTTATGAGTTATGAGAAAATCACTTTCATATGGAATACTTGTGAATCTGTACAGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTAT
GCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGATTGAAATCTTGAGTGACAAACA
GAGTCAACCTGTTTCACAACTGAAGTTTGTGACTGAAGCATGGATGCAGATAGTTGAATGTAGACGTGTGTTGAAGTGGACCTATGCATATGGCTATTACCTTCCTGAGCAGGAACAAGCTAAAAGGCAATTCTTTGAATATTTACAAGGTGAAGCTGAATCTGGGCTAGAAAGACTTCATCAATGTGCTGAAAAGGAAT
TGCAATTATATCTTGAAGGTGACATTCCTTCTAGGGAATTCAATGAATTCCGTACAAAACTTGCAGGGCTAACAAGTGTGACACGCAATTACTTTGAGAATCTTGTTCGAGCTCTGGAGAATGGTTTGTCAGATGTGAACAATTCACAAGCAACTAGCAGCAGAACCAGTACCTCAAAAGCTTCTAGCAGCAACAAGGGA
AAAGGTGGATTTTTAAAGGTTGGCAGTTCAAAAATTCGTTCAAGCAGGACTGCAGAGATTGCAAGTCGCTGGAGCTGCAGTCACTGTACATTTTCAAATGTGAGTTCGATGACAGCGTGCAGTATGTGTCACCATCCACGATGATCCAGCCCCTGATGACAAAAATATGTTGCCAGATATGAAGATTGCAAAGCTCTGTA
TTCAGCTCATTGCTTTTTTCATGTATGTCTTTCAGCTCCCATGTTGCAGATGAAATCTACTCTGTAAGGATACGTTTTTACTTCAACCTGTAAAATAGGATGCTGCATGCTCTTGTTTCCTGGGCTTGATTTAGGTTTTTATCCTCTGTTACCAGAAACGGGACAGGGACTTTTGTTTCCCATAAAAGACGGCTAAGAAA
CAGGACGGAACGGAAAATGCTGTTTTTTGTGGTCATTTCCGTTTCTGAGCATCCAAAACCGTTTTGTACGAAATGAAGGTAAACAATCATCGTCTCTTCGCTGGTTTTCCTCCGAGGGGTGTTGAGGGAAGCGTGGATCCACCCGTTGGCATTCTGTTAGAGAATGACAATTGTATCTTCTTTTAACTCAGCCTTGATTT
TCATTTTTATTGTTGATAACATCACTGATTCAGTTTCTCTGTACATGGATTTTATGCGCTTTACGGTGTAATGAAGTGATGTATGGATCATTATTCCTCGTACAATTATTAATTTCTTGAGCAGATATTGGATCTCCATTAAGTTTTAAAAGTTATGAGGGATTGCCAAATTGTTAATCTCTTTGTACAGGGGTTTTCTG
TGCTTGAGGCTGTAATGAAGTGATGATGCCTCATTATTCCTCGTACAATTACTGATTTCTTGAGAGAACAGCAAAGAGAAAAATGAGGCAGCTATTGGATCTCTTTTAAGTTGAAAATTTACAAGAGATTGCCAAATTGTCAAATAGTTTTGATAAGGGATTACCAAATGCCAGAATTTAGGGAGACAAACTGCTTTAAA
ATATTATGAAATCTATTAAAATAAATATTTTTGATGTTTTAAATGGCTTTGTTTTGATAAATAATAGTTGAACTTGTGGAAATCTCTCTAAATAGACGTTAAGTTTAGTGTTGATTTTTCTCTCCTTGCTTTTTATATGCAAAACCTCCATACTCCATACTCCAT

>TRINITY_DN124892_c0_g2_i10 from sample A1 / Example unspliced isoform:

GGAAGGAGTTGTGCGATACTTGTCTTCTTTCCCCTTATTGTTGGATTAGGAATTGTGTGAATTTATTTTTGATGGTTGATGTGAATTTTTCAATCTTAGCTATTACAAAATTTAGTTGTTGCTGTTTTTCATATTTGCTTATATCTCCAGTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAAATTCCTTGGAGA
AATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGgtttggttttgctttcctgttctcatctcatttctttagagcttgtaagactctgctgctgttgaagggtgtccactgcggctagcaaagagcttctaactttgtgatttcctgtgttgagagcagTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAA
ATGCAATCTGTGCAGGTATTAGGTGTACTTGCATTCTAATTGCACGTAAATAAATTTATGGGACTGCATGACTAAAGCCCTCATTATCTCTGTTAGGTTTAAAACTAAATCAG

Intron 2 GTATTGTGAA ... TGATGTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.15	19	4.5	9
A2	23.41	31	11	8
A3	35.34	16	8.5	9
A4	29.22	29	7	17
A5	25	6	1	3
A6	19.96	9	0.5	4
A7	17.43	34	5.5	9
A8	15.55	12	1.5	3
A9	18.68	15	2	5
A10	24.91	21	2	12

>TRINITY_DN101056_c1_g1_i6 from sample A4 / Example spliced isoform

AAACGGGCAGCGCGATATATTGGGAGTTGGTATGGTTAGCAGAGAAGGTAAAAAAGGAGAAATTAATGCAGAGCAGAAGAGAAGAGCAGCAACAGTTGAGGAAGAAGCAGGAAGCGGAGGTGGAGGCACCATCCCCATCGTCATACCACTCAGCTTGACTTGACTGGAACCCGAATGCGCAAGCTGGGGTTTACTTCAGC
AACCCATGTTCGAAACCCCTAAACCCCCCTGACACCAAAACAAATTTCTTCATCTGCTACTCTTTCCCACAAAATGGACTCGGAGGACGATATGCACGATGCCTACGACGGAGATTACCTTGACGACAGCGATGGAGGAGAAGGAGGACGAATTTTAGATTTTGACAACGACGATGACGTGGACGACGACGGCGATTATG
GCTTCGTCGATACGGCAGCAGACGATCACGACTCTACCTGCCGTTCCCAGGTGAACTACACAATTTTAAGTGAGGAAGACATTCTTCAATGTGAAGAAGAGGCAGCTACAAGTATTTCTACAGTTCTTTCCATACCAAGGGAGAATGCTGGCATACTTTTGCGGCATTTCAAATGGAGTGTCAGTAAAGTCAATGATGAA
TGGTTTGCAGACGAGGAGAATGTGCGGAAGACTGTGGGGCTGTTGGAAAAACCAATTGTAGAAATAAGATCAAATGTAAAGGAGTTAACTTGTGGTATATGCTTTGAAACTTGTGCTCGTGAAAGAATGAAAGCAGCTGTTTGTGGCCATTGCTTCTGTGACACATGCTGGGCAGGTTACATCCATACATCAATAAACGA
TGGACCTGGATGCCTTACATTACGATGTCCTGATCCGTCGTGTGTTGCTGCTGTTGGCGAAGATATGGTTTTTTCTTTGGTTTCTAATGAAGATAAAAAGAAGTACAGGCGCTACCTTCTTAGATCATATGTTGAAGACAACAGAAAGGCTAAGTGGTGCCCAGCACCCGACTGTGAATATGCTGTAGAATTTGTCCTTG
GAAGTGGCTGTTATGACATTTCTTGCAAATGCTCGTATGTCTTTTGCTGGAATTGTTTGGAGGAAGCTCATCGTCCTGTAGACTGTGATACAGTGGAAAGGTGGATCTTGAAGAACAGTGCAGAGTCAGAAAACATGAACTGGATACTTGCGAATTCAAAGCCATGCCCAAAGTGCAAGCGTCCAATCGAGAAAAATCAA
GGTTGCATGCATATTACCTGTACACCACCATGTAAATTCGAATTTTGCTGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTATGCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTGTGCGATACTTGTCTTCTTTCCCCTTATTGTTGGATTAGGAATTGTGTGAATTTATTTT
TGATGGTTGATGTGAATTTTTCAATCTTAGCTATTACAAAATTTAGTTGTTGCTGTTTTTCATATTTGCTTATATCTCCAGTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGGTTTGGTTTTGCTTTCCTGTTCTCATCTC
ATTTCTTTAGAGCTTGTAAGACTCTGCTGCTGTTGAAGGGTGTCCACTGCGGCTAGCAAAGAGCTTCTAACTTTGTGATTTCCTGTGTTGAGAGCAGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGGTATTAGGTGTACTTGCATTCTAATTGCACGTAAATAAATTTATGGGACTGCATG
ACTAAAGCCCTCATTATCTCTGTTAGGTTTAAAACTAAATCAGTGGCCTTTTGCCTATTGTTTATGAAAGTTTATGGAGATGTCTTCTTTGTTTCTGACGGCACTTTTCAGAAAGTTTAACAAGCTATATGTTTGGTTGTAGAATTTGGTTGAATTTTGTCGTCACCATGAGAAAGCTATGTAGCTAGCCTTGCTCCGAG
TGTCCAGGGTTTGTTGGTGCAGGTATAGGGATGCCATTTTTGAATTCAGACATCATGGGGTTGCTAGGGGAGGGTGTGTCAAATATATATGTATTTTTGTGTGCGTGCCCTGTGTAACATATGTATATATTATTGTACGTATATAAATATATTAATACTTAATAAGACATTGCGATAAGACGTTAATAATATTATATATT
ATACTCTATATATACATGTAACTTATATACAAACCCTGCATGTAACTTGTTTACCTGCACTCATGAACCTTGGCCACG

>TRINITY_DN101056_c1_g1_i13 from sample A4 / Example unspliced isoform:

AAATAAGATCAAATGTAAAGGAGGCAAGTAATCTTGGTGACTTGTGTGACAGCTATTTTAATCATGAAAATGCAATTGACTATGGATCAAAGGAATCCTTTGGTTTACTTCATATCATTAGACACACAGTGTTTCATGTTTGGCAGTTAACTTGTGGTATATGCTTTGAAACTTGTGCTCGTGAAAGAATGAAAGCAGCT
GTTTGTGGCCATTGCTTCTGTGACACATGCTGGGCAGgtattgtgaaaatgcctaaataagattaccaagttattgcattgtcggcaaggcttataactttatatttctgaagaagataataattcatgaaagctagaatcagcttttattacatagcggtgtagattacatatccactttcatacagcctctaaggttt
aaatgagtcccatagttgaatgcacgttgtgagttatgatctagggctaaactggatgatgcgaactttgcaaacatgtttactgcttttttaactcagcattattctttgctgttctattaatatatggtttacattttctttcttcccctctttgaggtatattttagacttttgttgtgttctgacattgaaaatga
acttattttctgttctttttcttctgagcattttcattcatgatgttgaagatcaaacgaggtatgattgagttaaccaatgagcaggttggaagtgatcttaactccttctctctgatgtgaagGTTACATCCATACATCAATAAACGATGGACCTGGATGCCTTACATTACGATGTCCTGATCCGTCGTGTGTTGCTG
CTGTTGGCGAAGATATGGTTTTTTCTTTGGTTTCTAATGAAGATAAAAAGAAGTACAGGCGCTACCTTCTTAGATCATATGTTGAAGACAACAGAAAGGTGGATATCATTTCCTGACATGGTTGTATCAATAATCCATTTTTATGCCCTATTCTCTAGTTGCAAATGCACAAATGAATATGTTAGTTCTGCTGTCTATTA
CTAGGAATATCAGC

Intron 3 GCAAGTAATC ... TGTTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.11	35.6	2	4
A2	42.22	17.8	3	13
A3	15.08	28.2	4	5
A4	18.24	31.4	2	7
A5	29.45	12	0	5
A6	16.97	9.8	1	2
A7	6.48	29	0	2
A8	0	25.8	0	0
A9	0	26.8	0	0
A10	0	18.8	0	0

>TRINITY_DN101056_c1_g1_i6 from sample A4 / Example spliced isoform

AAACGGGCAGCGCGATATATTGGGAGTTGGTATGGTTAGCAGAGAAGGTAAAAAAGGAGAAATTAATGCAGAGCAGAAGAGAAGAGCAGCAACAGTTGAGGAAGAAGCAGGAAGCGGAGGTGGAGGCACCATCCCCATCGTCATACCACTCAGCTTGACTTGACTGGAACCCGAATGCGCAAGCTGGGGTTTACTTCAGC
AACCCATGTTCGAAACCCCTAAACCCCCCTGACACCAAAACAAATTTCTTCATCTGCTACTCTTTCCCACAAAATGGACTCGGAGGACGATATGCACGATGCCTACGACGGAGATTACCTTGACGACAGCGATGGAGGAGAAGGAGGACGAATTTTAGATTTTGACAACGACGATGACGTGGACGACGACGGCGATTATG
GCTTCGTCGATACGGCAGCAGACGATCACGACTCTACCTGCCGTTCCCAGGTGAACTACACAATTTTAAGTGAGGAAGACATTCTTCAATGTGAAGAAGAGGCAGCTACAAGTATTTCTACAGTTCTTTCCATACCAAGGGAGAATGCTGGCATACTTTTGCGGCATTTCAAATGGAGTGTCAGTAAAGTCAATGATGAA
TGGTTTGCAGACGAGGAGAATGTGCGGAAGACTGTGGGGCTGTTGGAAAAACCAATTGTAGAAATAAGATCAAATGTAAAGGAGTTAACTTGTGGTATATGCTTTGAAACTTGTGCTCGTGAAAGAATGAAAGCAGCTGTTTGTGGCCATTGCTTCTGTGACACATGCTGGGCAGGTTACATCCATACATCAATAAACGA
TGGACCTGGATGCCTTACATTACGATGTCCTGATCCGTCGTGTGTTGCTGCTGTTGGCGAAGATATGGTTTTTTCTTTGGTTTCTAATGAAGATAAAAAGAAGTACAGGCGCTACCTTCTTAGATCATATGTTGAAGACAACAGAAAGGCTAAGTGGTGCCCAGCACCCGACTGTGAATATGCTGTAGAATTTGTCCTTG
GAAGTGGCTGTTATGACATTTCTTGCAAATGCTCGTATGTCTTTTGCTGGAATTGTTTGGAGGAAGCTCATCGTCCTGTAGACTGTGATACAGTGGAAAGGTGGATCTTGAAGAACAGTGCAGAGTCAGAAAACATGAACTGGATACTTGCGAATTCAAAGCCATGCCCAAAGTGCAAGCGTCCAATCGAGAAAAATCAA
GGTTGCATGCATATTACCTGTACACCACCATGTAAATTCGAATTTTGCTGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTATGCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTGTGCGATACTTGTCTTCTTTCCCCTTATTGTTGGATTAGGAATTGTGTGAATTTATTTT
TGATGGTTGATGTGAATTTTTCAATCTTAGCTATTACAAAATTTAGTTGTTGCTGTTTTTCATATTTGCTTATATCTCCAGTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGGTTTGGTTTTGCTTTCCTGTTCTCATCTC
ATTTCTTTAGAGCTTGTAAGACTCTGCTGCTGTTGAAGGGTGTCCACTGCGGCTAGCAAAGAGCTTCTAACTTTGTGATTTCCTGTGTTGAGAGCAGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGGTATTAGGTGTACTTGCATTCTAATTGCACGTAAATAAATTTATGGGACTGCATG
ACTAAAGCCCTCATTATCTCTGTTAGGTTTAAAACTAAATCAGTGGCCTTTTGCCTATTGTTTATGAAAGTTTATGGAGATGTCTTCTTTGTTTCTGACGGCACTTTTCAGAAAGTTTAACAAGCTATATGTTTGGTTGTAGAATTTGGTTGAATTTTGTCGTCACCATGAGAAAGCTATGTAGCTAGCCTTGCTCCGAG
TGTCCAGGGTTTGTTGGTGCAGGTATAGGGATGCCATTTTTGAATTCAGACATCATGGGGTTGCTAGGGGAGGGTGTGTCAAATATATATGTATTTTTGTGTGCGTGCCCTGTGTAACATATGTATATATTATTGTACGTATATAAATATATTAATACTTAATAAGACATTGCGATAAGACGTTAATAATATTATATATT
ATACTCTATATATACATGTAACTTATATACAAACCCTGCATGTAACTTGTTTACCTGCACTCATGAACCTTGGCCACG

>TRINITY_DN101056_c1_g1_i13 from sample A4 / Example unspliced isoform:

AAATAAGATCAAATGTAAAGGAGgcaagtaatcttggtgacttgtgtgacagctattttaatcatgaaaatgcaattgactatggatcaaaggaatcctttggtttacttcatatcattagacacacagtgtttcatgtttggcagTTAACTTGTGGTATATGCTTTGAAACTTGTGCTCGTGAAAGAATGAAAGCAGCT
GTTTGTGGCCATTGCTTCTGTGACACATGCTGGGCAGGTATTGTGAAAATGCCTAAATAAGATTACCAAGTTATTGCATTGTCGGCAAGGCTTATAACTTTATATTTCTGAAGAAGATAATAATTCATGAAAGCTAGAATCAGCTTTTATTACATAGCGGTGTAGATTACATATCCACTTTCATACAGCCTCTAAGGTTT
AAATGAGTCCCATAGTTGAATGCACGTTGTGAGTTATGATCTAGGGCTAAACTGGATGATGCGAACTTTGCAAACATGTTTACTGCTTTTTTAACTCAGCATTATTCTTTGCTGTTCTATTAATATATGGTTTACATTTTCTTTCTTCCCCTCTTTGAGGTATATTTTAGACTTTTGTTGTGTTCTGACATTGAAAATGA
ACTTATTTTCTGTTCTTTTTCTTCTGAGCATTTTCATTCATGATGTTGAAGATCAAACGAGGTATGATTGAGTTAACCAATGAGCAGGTTGGAAGTGATCTTAACTCCTTCTCTCTGATGTGAAGGTTACATCCATACATCAATAAACGATGGACCTGGATGCCTTACATTACGATGTCCTGATCCGTCGTGTGTTGCTG
CTGTTGGCGAAGATATGGTTTTTTCTTTGGTTTCTAATGAAGATAAAAAGAAGTACAGGCGCTACCTTCTTAGATCATATGTTGAAGACAACAGAAAGGTGGATATCATTTCCTGACATGGTTGTATCAATAATCCATTTTTATGCCCTATTCTCTAGTTGCAAATGCACAAATGAATATGTTAGTTCTGCTGTCTATTA
CTAGGAATATCAGC

Intron 4 GTAAGTGAAG ... CCATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.08	30	0	9
A2	28.95	27	0	11
A3	20	12	0	3
A4	37.04	17	5	10
A5	5	19	1	1
A6	20	12	0	3
A7	21.74	18	0	5
A8	30.43	16	0	7
A9	4.55	21	0	1
A10	26.67	33	6	12

>TRINITY_DN101056_c1_g1_i6 from sample A4 / Example spliced isoform

AAACGGGCAGCGCGATATATTGGGAGTTGGTATGGTTAGCAGAGAAGGTAAAAAAGGAGAAATTAATGCAGAGCAGAAGAGAAGAGCAGCAACAGTTGAGGAAGAAGCAGGAAGCGGAGGTGGAGGCACCATCCCCATCGTCATACCACTCAGCTTGACTTGACTGGAACCCGAATGCGCAAGCTGGGGTTTACTTCAGC
AACCCATGTTCGAAACCCCTAAACCCCCCTGACACCAAAACAAATTTCTTCATCTGCTACTCTTTCCCACAAAATGGACTCGGAGGACGATATGCACGATGCCTACGACGGAGATTACCTTGACGACAGCGATGGAGGAGAAGGAGGACGAATTTTAGATTTTGACAACGACGATGACGTGGACGACGACGGCGATTATG
GCTTCGTCGATACGGCAGCAGACGATCACGACTCTACCTGCCGTTCCCAGGTGAACTACACAATTTTAAGTGAGGAAGACATTCTTCAATGTGAAGAAGAGGCAGCTACAAGTATTTCTACAGTTCTTTCCATACCAAGGGAGAATGCTGGCATACTTTTGCGGCATTTCAAATGGAGTGTCAGTAAAGTCAATGATGAA
TGGTTTGCAGACGAGGAGAATGTGCGGAAGACTGTGGGGCTGTTGGAAAAACCAATTGTAGAAATAAGATCAAATGTAAAGGAGTTAACTTGTGGTATATGCTTTGAAACTTGTGCTCGTGAAAGAATGAAAGCAGCTGTTTGTGGCCATTGCTTCTGTGACACATGCTGGGCAGGTTACATCCATACATCAATAAACGA
TGGACCTGGATGCCTTACATTACGATGTCCTGATCCGTCGTGTGTTGCTGCTGTTGGCGAAGATATGGTTTTTTCTTTGGTTTCTAATGAAGATAAAAAGAAGTACAGGCGCTACCTTCTTAGATCATATGTTGAAGACAACAGAAAGGCTAAGTGGTGCCCAGCACCCGACTGTGAATATGCTGTAGAATTTGTCCTTG
GAAGTGGCTGTTATGACATTTCTTGCAAATGCTCGTATGTCTTTTGCTGGAATTGTTTGGAGGAAGCTCATCGTCCTGTAGACTGTGATACAGTGGAAAGGTGGATCTTGAAGAACAGTGCAGAGTCAGAAAACATGAACTGGATACTTGCGAATTCAAAGCCATGCCCAAAGTGCAAGCGTCCAATCGAGAAAAATCAA
GGTTGCATGCATATTACCTGTACACCACCATGTAAATTCGAATTTTGCTGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTATGCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTGTGCGATACTTGTCTTCTTTCCCCTTATTGTTGGATTAGGAATTGTGTGAATTTATTTT
TGATGGTTGATGTGAATTTTTCAATCTTAGCTATTACAAAATTTAGTTGTTGCTGTTTTTCATATTTGCTTATATCTCCAGTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGGTTTGGTTTTGCTTTCCTGTTCTCATCTC
ATTTCTTTAGAGCTTGTAAGACTCTGCTGCTGTTGAAGGGTGTCCACTGCGGCTAGCAAAGAGCTTCTAACTTTGTGATTTCCTGTGTTGAGAGCAGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGGTATTAGGTGTACTTGCATTCTAATTGCACGTAAATAAATTTATGGGACTGCATG
ACTAAAGCCCTCATTATCTCTGTTAGGTTTAAAACTAAATCAGTGGCCTTTTGCCTATTGTTTATGAAAGTTTATGGAGATGTCTTCTTTGTTTCTGACGGCACTTTTCAGAAAGTTTAACAAGCTATATGTTTGGTTGTAGAATTTGGTTGAATTTTGTCGTCACCATGAGAAAGCTATGTAGCTAGCCTTGCTCCGAG
TGTCCAGGGTTTGTTGGTGCAGGTATAGGGATGCCATTTTTGAATTCAGACATCATGGGGTTGCTAGGGGAGGGTGTGTCAAATATATATGTATTTTTGTGTGCGTGCCCTGTGTAACATATGTATATATTATTGTACGTATATAAATATATTAATACTTAATAAGACATTGCGATAAGACGTTAATAATATTATATATT
ATACTCTATATATACATGTAACTTATATACAAACCCTGCATGTAACTTGTTTACCTGCACTCATGAACCTTGGCCACG

>TRINITY_DN101056_c1_g1_i7 from sample A4 / Example unspliced isoform:

GGGAGTTTAGTGCTTGAAGAAATAACAGGGGTTTTTAAAATAAAAAACCTCATTGGAGCTTATTTTTGTTTATTTCTGTAGGCTAAGTGGTGCCCAGCACCCGACTGTGAATATGCTGTAGAATTTGTCCTTGGAAGTGGCTGTTATGACATTTCTTGCAAATGCTCGTATGTCTTTTGCTGGAATgtaagtgaagttta
tgcttttgtttcaaggttcttcattggctaaagtagcaagtagttttttccattaactataccagataataatttccttttcaattttatagttttgaagtcaatgtttgtatttctttgtgattaaaccattttcagTGTTTGGAGGAAGCTCATCGTCCTGTAGACTGTGATACAGTGGAAAGGTGGATCTTGAAGAA
CAGTGCAGAGTCAGAAAACATGAACTGGATACTTGCGAATTCAAAGCCATGCCCAAAGTGCAAGCGTCCAATCGAGAAAAATCAAGGTTGCATGCATATTACCTGTACACCACCATGTAAATTCGAATTTTGCTGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTATGCCTGCAACCGAT
ATGAGACAGCAAAACAGGAAGGAGTT

Intron 5 GTATGTCTTT ... CTGTTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.72	5.4	10	6
A2	41.67	7	5	5
A3	6.67	7	0	1
A4	33.33	15	7	8
A5	41.67	7	5	5
A6	49.71	23.8	36	11
A7	43.21	31.6	21	27
A8	43.95	44	34	35
A9	40.7	32.8	24	21
A10	27.03	27	9	11

>TRINITY_DN124892_c0_g2_i3 from sample A1 / Example spliced isoform

AGTGCAAGCGTCCAATCGAGAAAAATCAAGGTTGCATGCATATTACCTGTACACCACCATGTAAATTCGAATTTTGCTGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTATGCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTTATGATGAAGCTGAGAGAAGAAGGGAGATG
GCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGATTGAAATCTTGAGTGACAAACAGAGTCAACCTGTTTCACAACTGAAGTTTGTGACTGAAGCATGGATGCAGATAGTTGAATGTAGACGTGT
GTTGAAGTGGACCTATGCATATGGCTATTACCTTCCTGAGCAGGAACAAGCTAAAAGGCAATTCTTTGAATATTTACAAGGTGAAGCTGAATCTGGGCTAGAAAGACTTCATCAATGTGCTGAAAAGGAATTGCAATTATATCTTGAAGGTGACATTCCTTCTAGGGAATTCAATGAATTCCGTACAAAACTTGCAGGGC
TAACAAGTGTGACACGCAATTACTTTGAGAATCTTGTTCGAGCTCTGGAGAATGGTTTGTCAGATGTGAACAATTCACAAGCAACTAGCAGCAGAACCAGTACCTCAAAAGCTTCTAGCAGCAACAAGGGAAAAGGTGGATTTTTAAAGGTTGGCAGTTCAAAAATTCGTTCAAGCAGGACTGCAGAGATTGCAAGTCGC
TGGAGCTGCAGTCACTGTACATTTTCAAATGTGAGTTCGATGACAGCGTGCAGTATGTGTCACCATCCACGATGATCCAGCCCCTGATGACAAAAATATGTTGCCAGATATGAAGATTGCAAAGCTCTGTATTCAGCTCATTGCTTTTTTCATAAACGGGACAGGGACTTTTGTTTCCCATAAAAGACGGCTAAGAAACA
GGACGGAACGGAAAATGCTGTTTTTTGTGGTCATTTCCGTTTCTGAGCATCCAAAACCGTTTTGTACGAAATGAAGGTAAACAATCATCGTCTCTTCGCTGGTTTTCCTCCGAGGGGTGTTGAGGGAAGCGTGGATCCACCCGTTGGCATTCTGTTAGAGAATGACAATTGTATCTTCTTTTAACTCAGCCTTGATTTTC
ATTTTTATTGTTGATAACATCACTGATTCAGTTTCTCTGTACATGGATTTTATGCGCTTTACGGTGTAATGAAGTGATGTATGGATCATTATTCCTCGTACAATTATTAATTTCTTGAGCAGATATTGGATCTCCATTAAGTTTTAAAAGTTATGAGGGATTGCCAAATTGTTAATCTCTTTGTACAGGGGTTTTCTGTG
CTTGAGGCTGTAATGAAGTGATGATGCCTCATTATTCCTCGTACAATTACTGATTTCTTGAGAGAACAGCAAAGAGAAAAATGAGGCAGCTATTGGATCTCTTTTAAGTTGAAAATTTACAAGAGATTGCCAAATTGTCAAATAGTTTTGATAAGGGATTACCAAATGCCAGAATTTAGGGAGACAAACTGCTTTAAAAT
ATTATGAAATCTATTAAAATAAATATTTTTGATGTTTTAAATGGCTTTGTTTTGATAAATAATAGTTGAACTTGTGGAAATCTCTCTAAATAGACGTTAAGTTTAGTGTTGATTTTTCTCTCCTTGCTTTTTATATGCAAAACCTCCATACTCCATACTCCAT

>TRINITY_DN124892_c0_g2_i5 from sample A1 / Example unspliced isoform:

TTGACTACTATACAGGGATGATATGAGTGAAAACATGATTTGAGCTTCAAATCACAAATGCAAATAACTGTTCATGCCTTTTGAACTAAAGGTGTTTATGAGTTATGAGAAAATCACTTTCATATGGAATACTTGTGAATCTGTACAGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTAT
GCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGATTGAAATCTTGAGTGACAAACA
GAGTCAACCTGTTTCACAACTGAAGTTTGTGACTGAAGCATGGATGCAGATAGTTGAATGTAGACGTGTGTTGAAGTGGACCTATGCATATGGCTATTACCTTCCTGAGCAGGAACAAGCTAAAAGGCAATTCTTTGAATATTTACAAGGTGAAGCTGAATCTGGGCTAGAAAGACTTCATCAATGTGCTGAAAAGGAAT
TGCAATTATATCTTGAAGGTGACATTCCTTCTAGGGAATTCAATGAATTCCGTACAAAACTTGCAGGGCTAACAAGTGTGACACGCAATTACTTTGAGAATCTTGTTCGAGCTCTGGAGAATGGTTTGTCAGATGTGAACAATTCACAAGCAACTAGCAGCAGAACCAGTACCTCAAAAGCTTCTAGCAGCAACAAGGGA
AAAGGTGGATTTTTAAAGGTTGGCAGTTCAAAAATTCGTTCAAGCAGGACTGCAGAGATTGCAAGTCGCTGGAGCTGCAGTCACTGTACATTTTCAAATGTGAGTTCGATGACAGCGTGCAGTATGTGTCACCATCCACGATGATCCAGCCCCTGATGACAAAAATATGTTGCCAGATATGAAGATTGCAAAGCTCTGTA
TTCAGCTCATTGCTTTTTTCATgtatgtctttcagctcccatgttgcagatgaaatctactctgtaaggatacgtttttacttcaacctgtaaaataggatgctgcatgctcttgtttcctgggcttgatttaggtttttatcctctgttaccagAAACGGGACAGGGACTTTTGTTTCCCATAAAAGACGGCTAAGAAA
CAGGACGGAACGGAAAATGCTGTTTTTTGTGGTCATTTCCGTTTCTGAGCATCCAAAACCGTTTTGTACGAAATGAAGGTAAACAATCATCGTCTCTTCGCTGGTTTTCCTCCGAGGGGTGTTGAGGGAAGCGTGGATCCACCCGTTGGCATTCTGTTAGAGAATGACAATTGTATCTTCTTTTAACTCAGCCTTGATTT
TCATTTTTATTGTTGATAACATCACTGATTCAGTTTCTCTGTACATGGATTTTATGCGCTTTACGGTGTAATGAAGTGATGTATGGATCATTATTCCTCGTACAATTATTAATTTCTTGAGCAGATATTGGATCTCCATTAAGTTTTAAAAGTTATGAGGGATTGCCAAATTGTTAATCTCTTTGTACAGGGGTTTTCTG
TGCTTGAGGCTGTAATGAAGTGATGATGCCTCATTATTCCTCGTACAATTACTGATTTCTTGAGAGAACAGCAAAGAGAAAAATGAGGCAGCTATTGGATCTCTTTTAAGTTGAAAATTTACAAGAGATTGCCAAATTGTCAAATAGTTTTGATAAGGGATTACCAAATGCCAGAATTTAGGGAGACAAACTGCTTTAAA
ATATTATGAAATCTATTAAAATAAATATTTTTGATGTTTTAAATGGCTTTGTTTTGATAAATAATAGTTGAACTTGTGGAAATCTCTCTAAATAGACGTTAAGTTTAGTGTTGATTTTTCTCTCCTTGCTTTTTATATGCAAAACCTCCATACTCCATACTCCAT

Intron 6 GTGCGATACT ... ATATCTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.8	8.8	3.4	7.5
A2	28.31	10.9	3.1	4.4
A3	13.37	8.3	1.8	1.4
A4	31.58	18.4	3.7	8.3
A5	22.08	4	2	1.2
A6	9.88	14.3	1.1	1.9
A7	10.86	19.9	0.7	2.5
A8	26.28	22.5	7.2	7.5
A9	6.59	18.6	4.3	1.3
A10	12.31	8.7	1.1	1

>TRINITY_DN124892_c0_g2_i5 from sample A1 / Example spliced isoform

TTGACTACTATACAGGGATGATATGAGTGAAAACATGATTTGAGCTTCAAATCACAAATGCAAATAACTGTTCATGCCTTTTGAACTAAAGGTGTTTATGAGTTATGAGAAAATCACTTTCATATGGAATACTTGTGAATCTGTACAGGCTTTGTCTGGGTGCCTGGTCAGATCATGGTGAAAGAACAGGTGGTTTTTAT
GCCTGCAACCGATATGAGACAGCAAAACAGGAAGGAGTTTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAAATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGATTGAAATCTTGAGTGACAAACA
GAGTCAACCTGTTTCACAACTGAAGTTTGTGACTGAAGCATGGATGCAGATAGTTGAATGTAGACGTGTGTTGAAGTGGACCTATGCATATGGCTATTACCTTCCTGAGCAGGAACAAGCTAAAAGGCAATTCTTTGAATATTTACAAGGTGAAGCTGAATCTGGGCTAGAAAGACTTCATCAATGTGCTGAAAAGGAAT
TGCAATTATATCTTGAAGGTGACATTCCTTCTAGGGAATTCAATGAATTCCGTACAAAACTTGCAGGGCTAACAAGTGTGACACGCAATTACTTTGAGAATCTTGTTCGAGCTCTGGAGAATGGTTTGTCAGATGTGAACAATTCACAAGCAACTAGCAGCAGAACCAGTACCTCAAAAGCTTCTAGCAGCAACAAGGGA
AAAGGTGGATTTTTAAAGGTTGGCAGTTCAAAAATTCGTTCAAGCAGGACTGCAGAGATTGCAAGTCGCTGGAGCTGCAGTCACTGTACATTTTCAAATGTGAGTTCGATGACAGCGTGCAGTATGTGTCACCATCCACGATGATCCAGCCCCTGATGACAAAAATATGTTGCCAGATATGAAGATTGCAAAGCTCTGTA
TTCAGCTCATTGCTTTTTTCATGTATGTCTTTCAGCTCCCATGTTGCAGATGAAATCTACTCTGTAAGGATACGTTTTTACTTCAACCTGTAAAATAGGATGCTGCATGCTCTTGTTTCCTGGGCTTGATTTAGGTTTTTATCCTCTGTTACCAGAAACGGGACAGGGACTTTTGTTTCCCATAAAAGACGGCTAAGAAA
CAGGACGGAACGGAAAATGCTGTTTTTTGTGGTCATTTCCGTTTCTGAGCATCCAAAACCGTTTTGTACGAAATGAAGGTAAACAATCATCGTCTCTTCGCTGGTTTTCCTCCGAGGGGTGTTGAGGGAAGCGTGGATCCACCCGTTGGCATTCTGTTAGAGAATGACAATTGTATCTTCTTTTAACTCAGCCTTGATTT
TCATTTTTATTGTTGATAACATCACTGATTCAGTTTCTCTGTACATGGATTTTATGCGCTTTACGGTGTAATGAAGTGATGTATGGATCATTATTCCTCGTACAATTATTAATTTCTTGAGCAGATATTGGATCTCCATTAAGTTTTAAAAGTTATGAGGGATTGCCAAATTGTTAATCTCTTTGTACAGGGGTTTTCTG
TGCTTGAGGCTGTAATGAAGTGATGATGCCTCATTATTCCTCGTACAATTACTGATTTCTTGAGAGAACAGCAAAGAGAAAAATGAGGCAGCTATTGGATCTCTTTTAAGTTGAAAATTTACAAGAGATTGCCAAATTGTCAAATAGTTTTGATAAGGGATTACCAAATGCCAGAATTTAGGGAGACAAACTGCTTTAAA
ATATTATGAAATCTATTAAAATAAATATTTTTGATGTTTTAAATGGCTTTGTTTTGATAAATAATAGTTGAACTTGTGGAAATCTCTCTAAATAGACGTTAAGTTTAGTGTTGATTTTTCTCTCCTTGCTTTTTATATGCAAAACCTCCATACTCCATACTCCAT

>TRINITY_DN124892_c0_g2_i6 from sample A1 / Example unspliced isoform:

GGAAGGAGTTgtgcgatacttgtcttctttccccttattgttggattaggaattgtgtgaatttatttttgatggttgatgtgaatttttcaatcttagctattacaaaatttagttgttgctgtttttcatatttgcttatatctccagTATGATGAAGCTGAGAGAAGAAGGGAGATGGCAAAAAATTCCTTGGAGAA
ATACACTCATTACTATGAAAGATGGGCTACAAATGAATCGTCCAGGGCAAAGGCACTTGCTGATTTGCGGCAAATGCAATCTGTGCAGATTGAAATCTTGAGTGACAAACAGAGTCAACCTGTTTCACAACTGAAGTTTGTGACTGAAGCATGGATGCAGATAGTTGAATGTAGACGTGTGTTGAAGTGGACCTATGCAT
ATGGCTATTACCTTCCTGAGCAGGAACAAGCTAAAAGGCAATTCTTTGAATATTTACAAGGTGAAGCTGAATCTGGGCTAGAAAGACTTCATCAATGTGCTGAAAAGGAATTGCAATTATATCTTGAAGGTGACATTCCTTCTAGGGAATTCAATGAATTCCGTACAAAACTTGCAGGGCTAACAAGTGTGACACGCAAT
TACTTTGAGAATCTTGTTCGAGCTCTGGAGAATGGTTTGTCAGATGTGAACAATTCACAAGCAACTAGCAGCAGAACCAGTACCTCAAAAGCTTCTAGCAGCAACAAGGGAAAAGGTGGATTTTTAAAGGTTGGCAGTTCAAAAATTCGTTCAAGCAGGACTGCAGAGATTGCAAGTCGCTGGAGCTGCAGTCACTGTAC
ATTTTCAAATGTGAGTTCGATGACAGCGTGCAGTATGTGTCACCATCCACGATGATCCAGCCCCTGATGACAAAAATATGTTGCCAGATATGAAGATTGCAAAGCTCTGTATTCAGCTCATTGCTTTTTTCATAAACGGGACAGGGACTTTTGTTTCCCATAAAAGACGGCTAAGAAACAGGACGGAACGGAAAATGCTG
TTTTTTGTGGTCATTTCCGTTTCTGAGCATCCAAAACCGTTTTGTACGAAATGAAGGTAAACAATCATCGTCTCTTCGCTGGTTTTCCTCCGAGGGGTGTTGAGGGAAGCGTGGATCCACCCGTTGGCATTCTGTTAGAGAATGACAATTGTATCTTCTTTTAACTCAGCCTTGATTTTCATTTTTATTGTTGATAACAT
CACTGATTCAGTTTCTCTGTACATGGATTTTATGCGCTTTACGGTGTAATGAAGTGATGTATGGATCATTATTCCTCGTACAATTATTAATTTCTTGAGCAGATATTGGATCTCCATTAAGTTTTAAAAGTTATGAGGGATTGCCAAATTGTTAATCTCTTTGTACAGGGGTTTTCTGTGCTTGAGGCTGTAATGAAGTG
ATGATGCCTCATTATTCCTCGTACAATTACTGATTTCTTGAGAGAACAGCAAAGAGAAAAATGAGGCAGCTATTGGATCTCTTTTAAGTTGAAAATTTACAAGAGATTGCCAAATTGTCAAATAGTTTTGATAAGGGATTACCAAATGCCAGAATTTAGGGAGACAAACTGCTTTAAAATATTATGAAATCTATTAAAAT
AAATATTTTTGATGTTTTAAATGGCTTTGTTTTGATAAATAATAGTTGAACTTGTGGAAATCTCTCTAAATAGACGTTAAGTTTAGTGTTGATTTTTCTCTCCTTGCTTTTTATATGCAAAACCTCCATACTCCATACTCCAT