NA ----> NA

Intron 1 GTAGGGAGCA ... GTTGGAGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	5.98	52.5	3.8	7.3
A2	6.64	42.5	3.2	5.4
A3	12.88	63.5	10.4	10.2
A4	16.47	28.6	5.9	1.6
A5	8.41	10.8	1.2	0.9
A6	5.96	522.8	37.2	34.9
A7	7.99	449.2	43.8	40.2
A8	6.24	511.5	36.1	51.2
A9	8.11	389.9	38.2	29.9
A10	8.95	311.1	34.5	25.9

>TRINITY_DN101454_c0_g2_i3 from sample A3 / Example spliced isoform

ATTGTGTGTTAGAATCTCTGCTGTTAGTGCTTTCTCTGTGTCAACACCATGCCTCCATTAACTTCGAAGTCCATGGCTCTCGGGTCCATAGCCCCTGATTTTGAGCTGCCAGAACCTCTCACTGGTAAGGTTTGGAAGTTGGCCGACTTTGAAGGACATCCTGCACTGCTGGTGACGTTCATCTGCAATCACTGCCCATT
TGTTGTGCATTTGAAGAAGGATATTGTCAAGCTTGCAAATGATTACATGCCAAGGGGGCTTGCAATTGCAGCAATCTCTTCTAATTCTGCTGTCACATACCCTCAGGATGGTCCAGATTTTATGGCCGAAGATGCTAGGACGTTGGAGTATCCGTTCCCATATCTTTATGATGAGACACAAGAAGTTGCACGGGCTTATG
GAGCTGTTTGCACTCCTGAATTTTACCTATTCAAGAAGAATGAGAACCAGCCATTTGAGCTTGCTTATCATGGACAATTCGATGATTCACGACCAAATAATAACAAACCTATTACAGGAAGTGACATAAGGCAAGCTTTAGATTCTGTCCTGAGTGGCCAACCATTAAGTTCTGCACAAAAGCCAAGCGTTGGATGCAGT
ATTAAGTGGCACCCCAGTGAGAACTGAGAACGTCTCTTCCTCCTGACTGCAGTTAATATATATTCTCAATAAAAGTTGTCTCTGGTTGTATTCTTATATATGATTCTGTTAATAGTACTATTTGATTGGTCTTGTTGAACTCTGCACTACCTTATTCTTATATATGAACCTGTTAATAGTACTATTTAA

>TRINITY_DN101454_c0_g2_i1 from sample A3 / Example unspliced isoform:

ATTGTGTGTTAGAATCTCTGCTGTTAGTGCTTTCTCTGTGTCAACACCATGCCTCCATTAACTTCGAAGTCCATGGCTCTCGGGTCCATAGCCCCTGATTTTGAGCTGCCAGAACCTCTCACTGGTAAGGTTTGGAAGTTGGCCGACTTTGAAGGACATCCTGCACTGCTGGTGACGTTCATCTGCAATCACTGCCCATT
TGTTGTGCATTTGAAGAAGGATATTGTCAAGCTTGCAAATGATTACATGCCAAGGGGGCTTGCAATTGCAGCAATCTCTTCTAATTCTGCTGTCACATACCCTCAGGATGGTCCAGATTTTATGGCCGAAGATGCTAGGACGTTGGAGTATCCGTTCCCATATCTTTATGATGAGACACAAGAAGTTGCACGGGCTTATG
GAGCTGTTTGCACTCCTGAATTTTACCTATTCAAGAAGgtagggagcaatttaggtttcatccttcaatgtattgtatatacataaaattctttgacgctgtagaacaactcttcattgttcaatattatcctatgatggatttggacatggtactgttgtatgttggagtagAATGAGAACCAGCCATTTGAGCTTGCT
TATCATGGACAATTCGATGATTCACGACCAAATAATAACAAACCTATTACAGGAAGGTATGTTCTGAAGTGTGAAGCTGGAATTTCTTTTCTAATTACTTTCATTGTTTAGATCTGTGTAATTGGAGATGGATTCATTCACGGTCAAAATACTAAAATATATGTTTACAGTGACATAAGGCAAGCTTTAGATTCTGTCCT
GAGTGGCCAACCATTAAGTTCTGCACAAAAGCCAAGCGTTGGATGCAGTATTAAGTGGCACCCCAGTGAGAACTGAGAACGTCTCTTCCTCCTGACTGCAGTTAATATATATTCTCAATAAAAGTTGTCTCTGGTTGTATTCTTATATATGATTCTGTTAATAGTACTATTTGATTGGTCTTGTTGAACTCTGCACTACC
TTATTCTTATATATGAACCTGTTAATAGTACTATTTAA

Intron 2 GTATGTGACT ... ACTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.95	32	0	7
A2	15.87	53	6	10
A3	21.28	37	0	10
A4	18.18	18	0	4
A5	0	12	0	0
A6	20.74	474	3	124
A7	18.6	394	0	90
A8	17.68	568	0	122
A9	22.08	434	0	123
A10	13.59	267	0	42

>TRINITY_DN104700_c0_g1_i3 from sample A2 / Example spliced isoform

ATTTGTCATTGTGAATTGTGAGTTAGAATCTCTGCTGTTAGTGCTTTCTCTGTGTCAACACCATGCCTCCATTAACTTCGAAGTCCATGGCTCTCGGGTCCATAGCCCCTGATTTTGAGCTGCCAGAACCTCTCACTGGTAAGGTTTGGAAGTTGGCCGACTTTGAAGGACATCCTGCACTGCTGGTGACGTTCATCTGC
AATCACTGCCCATTTGTTGTGCATTTGAAGAAGGATATTGTCAAGCTTGCAAATGATTACATGCCAAGGGGGCTTGCAATTGCAGCAATCTCTTCTAATTCTGCTGTCACATACCCTCAGGATGGTCCAGATTTTATGGCCGAAGATGCTAGGACGTTGGAGTATCCGTTCCCATATCTTTATGATGAGACACAAGAAGT
TGCACGGGCTTATGGAGCTGTTTGCACTCCTGAATTTTACCTATTCAAGAAGAATGAGAACCAGCCATTTGAGCTTGCTTATCATGGACAATTCGATGATTCACGACCAAATAATAACAAACCTATTACAGGAAGTGACATAAGGCAAGCTTTAGATTCTGTCCTGAGTGGCCAACCATTAAGTTCTGCACAAAAGCCAA
GCGTTGGATGCAGTATTAAGTGGCACCCCAGTGAGAACTGAGAACGTCTCTTCCTCCTGACTGCAGTTAATATATATTCTCAATAAAAGTTGTCTCTGGTTGTATTCTTATATATGATTCTGTTAATAGTACTATTTGATTGGTCTTGTTGAACTCTGCACTACCTTATTCTTATATATGAACCTGTTAATAGTACTATT
TAATTGGTCTTGTTGAACTCTGCACTACCTTATTCTTATATATGAACCTGTTAATAGTACTATTTAATTGGTCTTGTTGAACTCTGCAC

>TRINITY_DN104700_c0_g1_i5 from sample A2 / Example unspliced isoform:

TCAAAATACTAAAATATATGTTTACAGTGACATAAGGCAAGCTTTAGATTCTGTCCTGAGTGGCCAACCATTAAGTTCTGCACAAAAGCCAAGgtatgtgactctttttccgcttctccatacaaaattgtttgcatgatagatagacaagagagtgtgtgctatcttaacacattgttatgatggacttatgcagCGTT
GGATGCAGTATTAAGTGGCACCCCAGTGAGAACTGAGAACGTCTCTTCCTCCTGACTGCAGTTAATATATATTCTCAATAAAAGTTGTCTCTGGTTGTATTCTTATATATGATTCTGTTAATAGTACTATTTGATTGGTCTTGTTGAACTCTGCACTACCTTATTCTTATATATGAACCTGTTAATAGTACTATTTAATT
GGTCTTGTTGAACTCTGCACTACCTTATTCTTATATATGAACCTGTTAATAGTACTATTTAATTGGTCTTGTTGAACTCTGCAC

Intron 3 GTATGTTCTG ... ATGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.52	35.6	1	7
A2	12.91	57.5	3	9.7
A3	13.64	35.7	9	6.5
A4	16.62	20.6	0	4.8
A5	10.19	12.9	0	1.6
A6	12.97	427.1	3	71.5
A7	10.75	379.2	6	50
A8	13.15	504.8	5	86.6
A9	14.36	368.8	10	70.3
A10	14.54	262.3	7	51.7

>TRINITY_DN101454_c0_g2_i3 from sample A3 / Example spliced isoform

ATTGTGTGTTAGAATCTCTGCTGTTAGTGCTTTCTCTGTGTCAACACCATGCCTCCATTAACTTCGAAGTCCATGGCTCTCGGGTCCATAGCCCCTGATTTTGAGCTGCCAGAACCTCTCACTGGTAAGGTTTGGAAGTTGGCCGACTTTGAAGGACATCCTGCACTGCTGGTGACGTTCATCTGCAATCACTGCCCATT
TGTTGTGCATTTGAAGAAGGATATTGTCAAGCTTGCAAATGATTACATGCCAAGGGGGCTTGCAATTGCAGCAATCTCTTCTAATTCTGCTGTCACATACCCTCAGGATGGTCCAGATTTTATGGCCGAAGATGCTAGGACGTTGGAGTATCCGTTCCCATATCTTTATGATGAGACACAAGAAGTTGCACGGGCTTATG
GAGCTGTTTGCACTCCTGAATTTTACCTATTCAAGAAGAATGAGAACCAGCCATTTGAGCTTGCTTATCATGGACAATTCGATGATTCACGACCAAATAATAACAAACCTATTACAGGAAGTGACATAAGGCAAGCTTTAGATTCTGTCCTGAGTGGCCAACCATTAAGTTCTGCACAAAAGCCAAGCGTTGGATGCAGT
ATTAAGTGGCACCCCAGTGAGAACTGAGAACGTCTCTTCCTCCTGACTGCAGTTAATATATATTCTCAATAAAAGTTGTCTCTGGTTGTATTCTTATATATGATTCTGTTAATAGTACTATTTGATTGGTCTTGTTGAACTCTGCACTACCTTATTCTTATATATGAACCTGTTAATAGTACTATTTAA

>TRINITY_DN101454_c0_g2_i1 from sample A3 / Example unspliced isoform:

ATTGTGTGTTAGAATCTCTGCTGTTAGTGCTTTCTCTGTGTCAACACCATGCCTCCATTAACTTCGAAGTCCATGGCTCTCGGGTCCATAGCCCCTGATTTTGAGCTGCCAGAACCTCTCACTGGTAAGGTTTGGAAGTTGGCCGACTTTGAAGGACATCCTGCACTGCTGGTGACGTTCATCTGCAATCACTGCCCATT
TGTTGTGCATTTGAAGAAGGATATTGTCAAGCTTGCAAATGATTACATGCCAAGGGGGCTTGCAATTGCAGCAATCTCTTCTAATTCTGCTGTCACATACCCTCAGGATGGTCCAGATTTTATGGCCGAAGATGCTAGGACGTTGGAGTATCCGTTCCCATATCTTTATGATGAGACACAAGAAGTTGCACGGGCTTATG
GAGCTGTTTGCACTCCTGAATTTTACCTATTCAAGAAGGTAGGGAGCAATTTAGGTTTCATCCTTCAATGTATTGTATATACATAAAATTCTTTGACGCTGTAGAACAACTCTTCATTGTTCAATATTATCCTATGATGGATTTGGACATGGTACTGTTGTATGTTGGAGTAGAATGAGAACCAGCCATTTGAGCTTGCT
TATCATGGACAATTCGATGATTCACGACCAAATAATAACAAACCTATTACAGGAAGgtatgttctgaagtgtgaagctggaatttcttttctaattactttcattgtttagatctgtgtaattggagatggattcattcacggtcaaaatactaaaatatatgtttacagTGACATAAGGCAAGCTTTAGATTCTGTCCT
GAGTGGCCAACCATTAAGTTCTGCACAAAAGCCAAGCGTTGGATGCAGTATTAAGTGGCACCCCAGTGAGAACTGAGAACGTCTCTTCCTCCTGACTGCAGTTAATATATATTCTCAATAAAAGTTGTCTCTGGTTGTATTCTTATATATGATTCTGTTAATAGTACTATTTGATTGGTCTTGTTGAACTCTGCACTACC
TTATTCTTATATATGAACCTGTTAATAGTACTATTTAA