NA ----> NA

Intron 1 GTCTGGAATA ... TTGGATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	52	1	13
A2	15.38	33	1.7	6
A3	18.03	50	1.3	11
A4	14.29	30	2.7	5
A5	14.29	30	1.3	5
A6	15.92	301	3	57
A7	19.13	304.3	0.7	72
A8	17.3	306	1	64
A9	17.98	237.3	0.7	52
A10	11.25	213	0.3	27

>TRINITY_DN113367_c0_g1_i2 from sample A1 / Example spliced isoform

CCAGGACCACAATACCATTTTCGTCGAGTTGGGCATTACTATTTTATTTGGGTTGTCTGAAAACAATTTCCTTTGTAATCGGGTAGTGCCCAATTCCATGGAGATTTCCTGAAATCACATTATTTTGCCGGATCTTTTTAATAAACGGAGAACTAAGATACGAAGAAGTTGAAGTGGGAGTCAAGTGATTATTGTACAAT
GGCTTGGGAATTTTTTGATCTGGAGAAACAATTTGCATTCTATGGAACTTATCACAGCCATTCAATGAATGTTCTCATTCATGCCATAATTGCGGGGCCACTATTTTTCAATAGCCTAGTATTGGCATCCTTCACCCCTGCTTTGGGTCTGCTTCCCTTCCCACCAGGGACCTTTCCATTCCAGGAGTACATGATATTGA
ATTTGAGTTTTGTTGTTGCTGTTGTCTATGCTCTCGTCTATATCATGTTGGATAAGAAGGCAGGTGCTCTGGCAGGAGCCCTTTGCCTTCTTTGTTGGGTCAGCAGCAATGCTTTTGCTCAGAGTTTGGGTTTCGCCTTAGCATGGAAGGTGGTTCTTGCTTCTGAATTGATATTTTTGGCACCTGGAATCATAGGACAC
AAAGTATTTGAGGAGCGAGCACCTGTGGTTTTGGATAATCTTATTGAAGTTTTCATGATGGAACAATTTTTTGTCTTCCTTGAGGTCCTCCAAAAGTTCTTTGGTTACGAACCTTACCCTGGTTTCCAGAAGGCTGTTCAAGCAAGAGTGCAAGCAAATATCAAGGAATGGACTGTGAAGGAGCAGAAGAAATCTTCATA
GTTGGCATGCCTGTTGCTTTTCTTCAAGACGAAACTTCGTCAACATGATGCATGCTTAATGGACTTCTTCTTACAGTAATTTATCTCATTCTACTGTAAAGTGCCACTATTAGCTCCTTTAAGGGGGGCATCAGTATAATGTTCTGACTTTTATGTGACTGTTTTATAATAGTCCTGAAAGGTGTTTACCTTCATTGGAA
TCATTTACTGGTATTCAGTCTTAATTCTGCAGTGAAGGAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGCTTGACAGTTTCCAGATTAGTAGTATTCAATCAGTCAATACAAATCTCTGTAGGGTGCTACTATTCGTACCATTCAGGAGGGCATTAGTATAATATTCTGACTGTAATGCAATTGTATTATTAATA
GTCCTGAAATGTGTTTAAACTTTATTTTCATTGGAATCATTGATGTGGTATTCAGTCTTAAATCTTCAGTGAAGTAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGGTTGACAGTTTCCACATTAGTCCAGTTGTACTCACTCCATCA

>TRINITY_DN113367_c0_g1_i3 from sample A1 / Example unspliced isoform:

CTAGGCTGTGTGCTGGCACTGACTAGCCAAGTTGGGACGTTGTAATGGTTGCACAGGGATTCTGGCACAGGACTTTGGTCGTTGGCATAAAAAATGTCTGGTGCAAGAGCAGAAGCTTGGGCACTTTCACTGATCTTGCCTGGGCTCTGGCACAGGACTGACATAGTTGAGCTGGAGAATGGATCATATTTATTTCTGAA
TGATTGACTATTGCTTTGTTTTTCTCAGGTGGTTCTTGCTTCTGAATTGATATTTTTGGCACCTGGAATCATAGGACACAAAGTATTTGAGGTACCCATATGAAACTATACAAATTTTTCCATGGCTGCTACATGTTGAATTGGAATCTCAGTACTTGTTTTGCAGGAGCGAGCACCTGTGGTTTTGGATAATCTTATTG
AAGTTTTCATGATGGAACAATTTTTTGTCTTCCTTGAGgtctggaatatgctttgcctcaataatattttccaaattgaagtgagaagcttttaaaatgtttttttggttagaagtgcacatctgaagtagtacacctgtgtttttggattcagGTCCTCCAAAAGTTCTTTGGTTACGAACCTTACCCTGGTTTCCAGA
AGGCTGTTCAAGCAAGAGTGCAAGCAAATATCAAGGAATGGACTGTGAAGGAGCAGAAGAAATCTTCATAGTTGGCATGCCTGTTGCTTTTCTTCAAGACGAAACTTCGTCAACATGATGCATGCTTAATGGACTTCTTCTTACAGTAATTTATCTCATTCTACTGTAAAGTGCCACTATTAGCTCCTTTAAGGGGGGCA
TCAGTATAATGTTCTGACTTTTATGTGACTGTTTTATAATAGTCCTGAAAGGTGTTTACCTTCATTGGAATCATTTACTGGTATTCAGTCTTAATTCTGCAGTGAAGGAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGCTTGACAGTTTCCAGATTAGTAGTATTCAATCAGTCAATACAAATCTCTGTAGGGT
GCTACTATTCGTACCATTCAGGAGGGCATTAGTATAATATTCTGACTGTAATGCAATTGTATTATTAATAGTCCTGAAATGTGTTTAAACTTTATTTTCATTGGAATCATTGATGTGGTATTCAGTCTTAAATCTTCAGTGAAGTAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGGTTGACAGTTTCCACATTA
GTCCAGTTGTACTCACTCCATCA

Intron 2 GTACCCATAT ... TGTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.56	37.5	5.7	9.8
A2	14.37	43.6	3	7.8
A3	22.5	46.7	6	15.7
A4	21.93	21.4	5	6.4
A5	21.29	29.1	5.6	8.4
A6	17.01	193	11	44.4
A7	15.38	226.9	7.6	45.6
A8	21.26	188.1	24.6	58.8
A9	16.05	172.8	3.7	37.3
A10	17.54	132.3	4	31.6

>TRINITY_DN113367_c0_g1_i2 from sample A1 / Example spliced isoform

CCAGGACCACAATACCATTTTCGTCGAGTTGGGCATTACTATTTTATTTGGGTTGTCTGAAAACAATTTCCTTTGTAATCGGGTAGTGCCCAATTCCATGGAGATTTCCTGAAATCACATTATTTTGCCGGATCTTTTTAATAAACGGAGAACTAAGATACGAAGAAGTTGAAGTGGGAGTCAAGTGATTATTGTACAAT
GGCTTGGGAATTTTTTGATCTGGAGAAACAATTTGCATTCTATGGAACTTATCACAGCCATTCAATGAATGTTCTCATTCATGCCATAATTGCGGGGCCACTATTTTTCAATAGCCTAGTATTGGCATCCTTCACCCCTGCTTTGGGTCTGCTTCCCTTCCCACCAGGGACCTTTCCATTCCAGGAGTACATGATATTGA
ATTTGAGTTTTGTTGTTGCTGTTGTCTATGCTCTCGTCTATATCATGTTGGATAAGAAGGCAGGTGCTCTGGCAGGAGCCCTTTGCCTTCTTTGTTGGGTCAGCAGCAATGCTTTTGCTCAGAGTTTGGGTTTCGCCTTAGCATGGAAGGTGGTTCTTGCTTCTGAATTGATATTTTTGGCACCTGGAATCATAGGACAC
AAAGTATTTGAGGAGCGAGCACCTGTGGTTTTGGATAATCTTATTGAAGTTTTCATGATGGAACAATTTTTTGTCTTCCTTGAGGTCCTCCAAAAGTTCTTTGGTTACGAACCTTACCCTGGTTTCCAGAAGGCTGTTCAAGCAAGAGTGCAAGCAAATATCAAGGAATGGACTGTGAAGGAGCAGAAGAAATCTTCATA
GTTGGCATGCCTGTTGCTTTTCTTCAAGACGAAACTTCGTCAACATGATGCATGCTTAATGGACTTCTTCTTACAGTAATTTATCTCATTCTACTGTAAAGTGCCACTATTAGCTCCTTTAAGGGGGGCATCAGTATAATGTTCTGACTTTTATGTGACTGTTTTATAATAGTCCTGAAAGGTGTTTACCTTCATTGGAA
TCATTTACTGGTATTCAGTCTTAATTCTGCAGTGAAGGAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGCTTGACAGTTTCCAGATTAGTAGTATTCAATCAGTCAATACAAATCTCTGTAGGGTGCTACTATTCGTACCATTCAGGAGGGCATTAGTATAATATTCTGACTGTAATGCAATTGTATTATTAATA
GTCCTGAAATGTGTTTAAACTTTATTTTCATTGGAATCATTGATGTGGTATTCAGTCTTAAATCTTCAGTGAAGTAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGGTTGACAGTTTCCACATTAGTCCAGTTGTACTCACTCCATCA

>TRINITY_DN113367_c0_g1_i3 from sample A1 / Example unspliced isoform:

CTAGGCTGTGTGCTGGCACTGACTAGCCAAGTTGGGACGTTGTAATGGTTGCACAGGGATTCTGGCACAGGACTTTGGTCGTTGGCATAAAAAATGTCTGGTGCAAGAGCAGAAGCTTGGGCACTTTCACTGATCTTGCCTGGGCTCTGGCACAGGACTGACATAGTTGAGCTGGAGAATGGATCATATTTATTTCTGAA
TGATTGACTATTGCTTTGTTTTTCTCAGGTGGTTCTTGCTTCTGAATTGATATTTTTGGCACCTGGAATCATAGGACACAAAGTATTTGAGgtacccatatgaaactatacaaatttttccatggctgctacatgttgaattggaatctcagtacttgttttgcagGAGCGAGCACCTGTGGTTTTGGATAATCTTATTG
AAGTTTTCATGATGGAACAATTTTTTGTCTTCCTTGAGGTCTGGAATATGCTTTGCCTCAATAATATTTTCCAAATTGAAGTGAGAAGCTTTTAAAATGTTTTTTTGGTTAGAAGTGCACATCTGAAGTAGTACACCTGTGTTTTTGGATTCAGGTCCTCCAAAAGTTCTTTGGTTACGAACCTTACCCTGGTTTCCAGA
AGGCTGTTCAAGCAAGAGTGCAAGCAAATATCAAGGAATGGACTGTGAAGGAGCAGAAGAAATCTTCATAGTTGGCATGCCTGTTGCTTTTCTTCAAGACGAAACTTCGTCAACATGATGCATGCTTAATGGACTTCTTCTTACAGTAATTTATCTCATTCTACTGTAAAGTGCCACTATTAGCTCCTTTAAGGGGGGCA
TCAGTATAATGTTCTGACTTTTATGTGACTGTTTTATAATAGTCCTGAAAGGTGTTTACCTTCATTGGAATCATTTACTGGTATTCAGTCTTAATTCTGCAGTGAAGGAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGCTTGACAGTTTCCAGATTAGTAGTATTCAATCAGTCAATACAAATCTCTGTAGGGT
GCTACTATTCGTACCATTCAGGAGGGCATTAGTATAATATTCTGACTGTAATGCAATTGTATTATTAATAGTCCTGAAATGTGTTTAAACTTTATTTTCATTGGAATCATTGATGTGGTATTCAGTCTTAAATCTTCAGTGAAGTAAATGCACCATTCTTTCCAATCTATTCTGAGCTCTGGTTGACAGTTTCCACATTA
GTCCAGTTGTACTCACTCCATCA