sp|P48489|PP1_ORYSJ ----> Serine/threonine-protein phosphatase PP1

Intron 1 GTATTTATTT ... TTTCACCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.25	15	1.8	1
A2	33.33	14	3	7
A3	0	10	1.3	0
A4	10	9	2.3	1
A5	60	4	4	6
A6	13	87	12.8	13
A7	14.19	72.6	8.3	12
A8	16.05	68	8	13
A9	12.28	100	9.8	14
A10	11.69	68	8.8	9

>TRINITY_DN43965_c2_g1_i4 from sample A6 / Example spliced isoform

TTTTGAACGATGTTATTAGCAGGTTGCTCGAGGTGAGAGGCTCGAAGCCTGGTAAGCAGGTTCAATTATCGGAGACCGAGGTCCGCCAACTATGCATTGTTTCAAGGACATTTTCCTGAAACAACCCAATCTGTTGGATCTAGAGGCTCCCATCAAGATCTGCGCTGCAAAGAATTCGATTTCACATAACAAGCTGCAGT
GCCATAACAAGCTGCCCATATGCTACCACATCCATCACTGTAGACTGCGAGCTCTTTTGTTGGCCAAGTCTGTATAGTTGGGGATCATTTTCTCTTGTGGGGTGAACAGAACCACCGGGGACGAGCAAAGAAGGAAGACAACTGGTGTATCACCCAAATCCCAAGTCAATTTGTGAAGATTTGGTCATCCCAACACTGTT
GGGCGTATCTTGGGCAGCCTGCCCTCTTTTTGTGACTATACGCTACCCTTTTGAGCGTAGAAAGTGACACTATAGTTGATTGTTGCCTACAACATCAAAGGGAATCAAGTCATGGCCGTTTAGAGGACTCCAGGAAGTCGTTTCAGCTGTACTAGAGGTCTGTGAGTCCACATGCACCTACAGGTCTGAAGTATTTTGCA
AATCTGCTTATTTGACTCACATTGTGACCTTGTTGTACCTGCCTGGGAAATAATGTCATTTCTTACTGTGTTGGGCACTATTTCTAGTATTAAATGAAGAGATCAGAATTGCTCTTTGGTGTTTATTTCATTGAATTCTCAGATTGTATGCC

>TRINITY_DN43965_c2_g1_i6 from sample A6 / Example unspliced isoform:

TTTTGAACGATGTTATTAGCAGGTTGCTCGAGGTGAGAGGCTCGAAGCCTGGTAAGCAGGTTCAATTATCGGAGACCGAGGTCCGCCAACTATGCATTGTTTCAAGGACATTTTCCTGAAACAACCCAATCTGTTGGATCTAGAGGCTCCCATCAAGATCTGCGgtatttatttttcacttatcacaattttacacaata
cccaattgattttaattgagatatcttgtctcttaatctcaaattgtttgtcacataaagtgttttgattgttgagctgatctctttcacccagCTGCAAAGAATTCGATTTCACATAACAAGCTGCAGTGCCATAACAAGCTGCCCATATGCTACCACATCCATCACTGTAGACTGCGAGCTCTTTTGTTGGCCAAGTC
TGTATAGTTGGGGATCATTTTCTCTTGTGGGGTGAACAGAACCACCGGGGACGAGCAAAGAAGGAAGACAACTGGTGTATCACCCAAATCCCAAGTCAATTTGTGAAGATTTGGTCATCCCAACACTGTTGGGCGTATCTTGGGCAGCCTGCCCTCTTTTTGTGACTATACGCTACCCTTTTGAGCGTAGAAAGTGACAC
TATAGTTGATTGTTGCCTACAACATCAAAGGGAATCAAGTCATGGCCGTTTAGAGGACTCCAGGAAGTCGTTTCAGCTGTACTAGAGGTCTGTGAGTCCACATGCACCTACAGGAGTGCAATGGTGAATTCACTGAGTATTTAATTGTGAAATCTGGAAATTGTATTTGATTATGGTACTGGAGTATGAATACAAAGAGG
TTGAAAATGGCGTAATTTCTAGCAGTGAGTATAGGAATACATGATTCTTGTGAAGAGGACCTAGAACTGCTTGAGAGAATTGAATGGTGGATTCAATTTGAGGTTAAGTTGATGGCATTGATGCCACATCAGATCTCTACATTGTGCAAATATAAATGGAATACTATGTGTTTAAATGCTGTGTCAAATCCTGTTGAATG
GATTGAATAGTGGATTTGTTCTGTGGAGGATTGCTCTGCAATGAGCAATATTGACAAGTTCATTTGTTCTTGAACATTTTGGATTGGTTATTGTTCAACGTTGGAGTTAACATTTGTGTTAAATTGCAAAAGGGGTGATCATATTTGGAAGTAGAGGAATGTGGAAAATGCTCATTTGGAGTTGCGTTGAAGGTAAATGG
TTGTTACACTATTCATTCATGTAATTTCAAGTTCCAGTTATTGATTGTTATGGTGATGCCAGCAGACTCGATTCCCTTGGGATTCAAATTCCTTTTATAAACCCAAACTGGGATCTATGGAAATGACTCTCTGGGGTAGAGCATCCGCTTCTGAGCAAATAGTTCAATTCTTTACATTTAAGTATTGTATTGAAAGCATG
AGACACATAATGAATATGGCCAGCTTGAAGATTGATTGTGAATGATGGTAGTTTTAATCATTGGTATTAAAATCTGCAATATGATGATGTACTCGACATACCATAAATTTAACTTGCGAACTATGCCAGATCATAGTAAGAATAGCCATTCTAGAGTTCAAGTTTACATCAGGTTATAGGATGCATCAATTGATGATATG
TGTATTTATAGAAATGGGAATGTGGACCTGATGTAAGGCAATTGCATAGTGGAATGGTATGCACCAGTCAATGTGTTTTCGTTTATTGAGAAGATAAATATAGGCACAATGGTTGTCTATATGATGTTGTGATTCTAGATCCAGAGATTGTAATATCCGATAACACCTTAAGATCCTGTTAGCTAGTGTATGTTTCATGT
AAAAGTTGCGTAGATCTGGATAGAGGAGGTTAGCAATTGAAGAGGAACGGCAATAACTCATTTCTCCCAGGTAAGAGCT

Intron 2 GTCTGTGAGT ... GCACCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.48	10	10	1
A2	35.29	11	9	3
A3	35.29	11	11	1
A4	33.33	5	3	2
A5	60	1	1	2
A6	45.33	41	36	32
A7	38.75	49	36	26
A8	46.07	48	44	38
A9	44.76	58	56	38
A10	38.6	35	30	14

>TRINITY_DN43965_c2_g1_i1 from sample A6 / Example spliced isoform

TTTTGAACGATGTTATTAGCAGGTTGCTCGAGGTGAGAGGCTCGAAGCCTGGTAAGCAGGTTCAATTATCGGAGACCGAGGTCCGCCAACTATGCATTGTTTCAAGGACATTTTCCTGAAACAACCCAATCTGTTGGATCTAGAGGCTCCCATCAAGATCTGCGCTGCAAAGAATTCGATTTCACATAACAAGCTGCAGT
GCCATAACAAGCTGCCCATATGCTACCACATCCATCACTGTAGACTGCGAGCTCTTTTGTTGGCCAAGTCTGTATAGTTGGGGATCATTTTCTCTTGTGGGGTGAACAGAACCACCGGGGACGAGCAAAGAAGGAAGACAACTGGTGTATCACCCAAATCCCAAGTCAATTTGTGAAGATTTGGTCATCCCAACACTGTT
GGGCGTATCTTGGGCAGCCTGCCCTCTTTTTGTGACTATACGCTACCCTTTTGAGCGTAGAAAGTGACACTATAGTTGATTGTTGCCTACAACATCAAAGGGAATCAAGTCATGGCCGTTTAGAGGACTCCAGGAAGTCGTTTCAGCTGTACTAGAGATCACAACAATTGATGACCCCATACATGAAGATCGAAATTATG
TTGAGCCTGCTTCAAATATAAGAGAAGAATAAGATCATGATGATGATGACGATGGTGATGAGGTTGATCAAGATGACGAGTTTGATGATCATGCCAATGATCTTGATGATGATGTTGATGGTGCTAGCTAGCTACTAATGCTATGCATGAG

>TRINITY_DN43965_c2_g1_i5 from sample A6 / Example unspliced isoform:

TTTTGAACGATGTTATTAGCAGGTTGCTCGAGGTGAGAGGCTCGAAGCCTGGTAAGCAGGTTCAATTATCGGAGACCGAGGTCCGCCAACTATGCATTGTTTCAAGGACATTTTCCTGAAACAACCCAATCTGTTGGATCTAGAGGCTCCCATCAAGATCTGCGCTGCAAAGAATTCGATTTCACATAACAAGCTGCAGT
GCCATAACAAGCTGCCCATATGCTACCACATCCATCACTGTAGACTGCGAGCTCTTTTGTTGGCCAAGTCTGTATAGTTGGGGATCATTTTCTCTTGTGGGGTGAACAGAACCACCGGGGACGAGCAAAGAAGGAAGACAACTGGTGTATCACCCAAATCCCAAGTCAATTTGTGAAGATTTGGTCATCCCAACACTGTT
GGGCGTATCTTGGGCAGCCTGCCCTCTTTTTGTGACTATACGCTACCCTTTTGAGCGTAGAAAGTGACACTATAGTTGATTGTTGCCTACAACATCAAAGGGAATCAAGTCATGGCCGTTTAGAGGACTCCAGGAAGTCGTTTCAGCTGTACTAGAGgtctgtgagtccacatgcacctacagATCACAACAATTGATGA
CCCCATACATGAAGATCGAAATTATGTTGAGCCTGCTTCAAATATAAGAGAAGAATAAGATCATGATGATGATGACGATGGTGATGAGGTTGATCAAGATGACGAGTTTGATGATCATGCCAATGATCTTGATGATGATGTTGATGGTGCTAGCTAGCTACTAATGCTATGCATGAG

Intron 3 GTAAGAACAA ... TTATTAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.17	7.2	26.3	71
A2	87	9.4	38.2	87
A3	94.5	4.4	40.2	105
A4	93.27	3.5	23.5	73
A5	87.47	4.3	18.3	42
A6	78.34	49.9	94.9	265
A7	83.43	44.8	128.1	321
A8	80.69	37.6	80.9	231
A9	82.39	37.4	96	250
A10	83.82	42	137	290

>TRINITY_DN133720_c2_g1_i1 from sample A1 / Example spliced isoform

TTCTCTATCGACAACTTTCTCTTTACACAACTAAATTCTTTGGGAATTGGGAGGCCTAAAGCTCAGCAGAGCGGGAGGAGACTGCTCTGCAGCTCAATGGTTAGCTCTCGGGGTTCTTCAAGGTGGAGTTCCAGTTCTCTATCGACAACTTTCTCTTTACACAACTAAATTCTTTGGGAATTGGGAGGCCTAAAGCTCAG
CAGAGCGGGAGGGGACGAGCAACACTGAAAACAATAAAGCTAATAATAAGCTAATGGCAGTGGTTGCTCGAGGTGAGAGGCTCGAAGCCTGGTAAGCAGGTTCAATTATCGGAGACCGAGGTCCGCCAACTATGCATTGTTTCAAGGACATTTTCCTGAAACAACCCAATCTGTTGGATCTAGAGGCTCCCATCAAGATC
TGCGCTGCAAAGAATTCGATTTCACATAACAAGCTGCAGTGCCATAACAAGCTGCCCATATGCTACCACATCCATCACTGTAGACTGCGAGCTCTTTTGTTGGCCAAGTCTGTATAGTTGGGGATCATTTTCTCTTGTGGGGTGAACAGAACCACCGGGGACGAGCAAAGAAGGAAGACAACTGGTGTATCACCCAAATC
CCAAGTCAATTTGTGAAGATTTGGTCATCCCAACACTGTTGGGCGTATCTTGGGCAGCCTGCCCTCTTTTTGTGACTATACGCTACCCTTTTGAGCGTAGAAAGTGACACTATAGTTGATTGTTGCCTACAACATCAAAGGGAATCAAGTCATGGCCGTTTAGAGGACTCCAGGAAGTCGTTTCAGCTGTACTAGAGGTC
TGTGAGTCCACATGCACCT

>TRINITY_DN133720_c2_g1_i2 from sample A1 / Example unspliced isoform:

TTCTCTATCGACAACTTTCTCTTTACACAACTAAATTCTTTGGGAATTGGGAGGCCTAAAGCTCAGCAGAGCGGGAGGAGACTGCTCTGCAGCTCAATGGTTAGCTCTCGGGGTTCTTCAAGGTGGAGTTCCAGTTCTCTATCGACAACTTTCTCTTTACACAACTAAATTCTTTGGGAATTGGGAGGCCTAAAGCTCAG
CAGAGCGGGAGGGGACGAGCAACACTGAAAACAATAAAGCTAATAATAAGCTAATGGCAGTGgtaagaacaaaggaatgagagcattatttatgaaagaaggattgaatggaacctgaggttttgaacgatgttattagcagGTTGCTCGAGGTGAGAGGCTCGAAGCCTGGTAAGCAGGTTCAATTATCGGAGACCGAG
GTCCGCCAACTATGCATTGTTTCAAGGACATTTTCCTGAAACAACCCAATCTGTTGGATCTAGAGGCTCCCATCAAGATCTGCGCTGCAAAGAATTCGATTTCACATAACAAGCTGCAGTGCCATAACAAGCTGCCCATATGCTACCACATCCATCACTGTAGACTGCGAGCTCTTTTGTTGGCCAAGTCTGTATAGTTG
GGGATCATTTTCTCTTGTGGGGTGAACAGAACCACCGGGGACGAGCAAAGAAGGAAGACAACTGGTGTATCACCCAAATCCCAAGTCAATTTGTGAAGATTTGGTCATCCCAACACTGTTGGGCGTATCTTGGGCAGCCTGCCCTCTTTTTGTGACTATACGCTACCCTTTTGAGCGTAGAAAGTGACACTATAGTTGAT
TGTTGCCTACAACATCAAAGGGAATCAAGTCATGGCCGTTTAGAGGACTCCAGGAAGTCGTTTCAGCTGTACTAGAGATCACAACAATTGATGACCCCATACATGAAGATCGAAATTATGTTGAGCCTGCTTCAAATATAAGAGAAGAATAAGATCATGATGATGATGACGATGGTGATGAGGTTGATCAAGATGACGAG
TTTGATGATCATGCCAATGATCTTGATGATGATGTTGATGGTGCTAGCTAGCTACTAATGCTATGCATGAGACATTTTGGATCATATTTACATGTATTTTTGTTGATAGATAACTATGCTATATTTATTATGGTGACTTTTATTTCTATATATGGAGATCTTACAACCTATTTCTTGTCAGATATATATATTTGACTTAT
TTTTATTTTATTATAATGATATCCATGAAAGC