sp|P20027|MYB3_HORVU ----> Myb-related protein Hv33

Intron 1 GTAATCCTCC ... CTCTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.8	468	216	790
A2	61.91	491	209	798
A3	62.09	511	225	837
A4	65	350	164	650
A5	58.97	320	114	460
A6	56.78	4054	1018	5325
A7	59.7	4076	1697	6037
A8	58.86	3572	1330	5111
A9	57.59	3861	1481	5242
A10	58.75	3323	1367	4732

>TRINITY_DN45537_c0_g1_i11 from sample A6 / Example spliced isoform

ACTCACTCCTGTACGTGTTGTATGCCTGCCACTTCCCACTCTGGAATTAAACCATCATCGCTCACCTCCACTCCGCGATGTACCCCGCTCAACACCATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGGTCCACGAAAAGGAGGTTCCGACTCCACACCAGATTCAGATG
GAACCGATTTCAAGGCTAATGGTGGAGACAACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGAAACCGATGCTATCTTGTGGCGTGTGGCAACAGTTTAAAGTTCCTCCTTGAACCTTATATGAAG
CTCCCATGGCGGACGCTAACATCCTAGACGGCCAAGAAATCGAAAACCCCAAATCCGCCGAACAGATCCCCCAGTCCAGTGTGCAGGAGCA

>TRINITY_DN45537_c0_g1_i8 from sample A6 / Example unspliced isoform:

CAGCGGTTAGATTTTAATGATAGAAGAATATGCTTCGTCTAGTGCATTGATGAAGCACTCAGATTTGCTGTGTTAACTCATGGACAGCTTTAAATTCAAACGACACATATTGTTTAAAATAATTTATTTACAAATGCTGTATGCCTGCCACTTCCCACTCACCTCCACTCCGCGCTGTACCCTCGCTGTACCCCCGCTCG
ACACCATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGgtaatcctccatttttttttgtttaatcctagcctcttgccaggtgggagcttcaacctgttaaattaatcggagcctcttgccagGTCCACGAAAAGGAGGTTCCGACTCCACACCAGATTCAGATGGAACC
GATTTCAAGGCTAATGGTGGAGACAACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGAAACCGATGCTATCTTGTGGCGTGTGGCAACAGTTTAAAGTTCCTCCTTGAACCTTATATGAAGCTCCC
ATGGCGGACGCTAACATCCTAGACGGCCAAGAAATCGAAAACCCCAAATCCGCCGAACAGATCCCCCAGTCCAGTGTGCAGGAGCA

Intron 2 GTTTCTTCCT ... TTAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.04	46	726	207
A2	94.74	38	685	162
A3	94.76	40	724	146
A4	97.18	14	482	123
A5	94.74	20	360	78
A6	99.14	69	7922	64
A7	97.53	167	6597	123
A8	98.87	94	8219	82
A9	98.77	81	6512	99
A10	97.74	110	4759	139

>TRINITY_DN124100_c2_g1_i1 from sample A3 / Example spliced isoform

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGATATTCAAATGTTGAAAGATGCATATCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCACGTTATGAAAGATGCATCGATAATACATGAAACATCTCACGTTATGAAAGATGCATCGATTG
AAGTTCTACTGCAGTATTATGGGCGCACTACAATTATGGTTTTATTTCATTCAATGTTGCAGG

>TRINITY_DN124100_c2_g1_i14 from sample A3 / Example unspliced isoform:

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGgtttcttcctatcgccaacattagccgcatcatgaagataaagtttctgcatcttcttcattttcttgactatagtttcgttatatcttggaccctgcatggccatcttcactggcttgaatttctttg
gcagcctaaaccattcatgaagctaatctctatttcttggtccggttcactcgatttcttgatttttttaaataaactttcaataataatgatgcgcaagattttatgggggggacagttgttttcaaaatatttgttaataaaaaatatttcagatttattttactattagatttgtgatttattagataacgattttg
aattttttgaaattaactacagATATTCAAATGTTGAAATATTTTATGTTGAAAGATGCATCTCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCACGTTATGAAAGATGCATCGATAATACATGAAACATCTCACGTTATGAAAGATGCATCGATTGAAGTTCTACTGCAGTATTATGGGCGCACTACAAT
TATGGTTTTATTTCATTCAATGTTGCAGGTATGCTGCCTGTAATGTTCTCTTTTTGCTTTCAAAGAAT

Intron 3 GTCCGGTTCA ... TTAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.91	227	611	89
A2	70.77	223	540	84
A3	70.52	242	579	64
A4	70.21	143	337	64
A5	70.54	119	285	34
A6	75.58	3165	9797	32
A7	75.66	2378	7390	78
A8	77.46	3422	11762	44
A9	77.85	2526	8878	54
A10	74.45	1439	4192	72

>TRINITY_DN124100_c2_g1_i17 from sample A3 / Example spliced isoform

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGGTTTCTTCCTATCGCCAACATTAGCCGCATCATGAAGATAAAGTTTCTGCATCTTCTTCATTTTCTTGACTATAGTTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCTTTG
GCAGCCTAAACCATTCATGAAGCTAATCTCTATTTCTTGATATTCAAATGTTGAAAGATGCATATCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCACGTTATGAAAGATGCATCGATAATACATGAAACATCTCACGTTATGAAAGATGCATCGATTGAAGTTCTACTGCAGTATTATGGGCGCACTACA
ATTATGGTTTTATTTCATTCAATGTTGCAGG

>TRINITY_DN124100_c2_g1_i12 from sample A3 / Example unspliced isoform:

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGGTTTCTTCCTATCGCCAACATTAGCCGCATCATGAAGATAAAGTTTCTGCATCTTCTTCATTTTCTTGACTATAGTTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCTTTG
GCAGCCTAAACCATTCATGAAGCTAATCTCTATTTCTTGgtccggttcactcgatttcttgatttttttaaataaactttcaataataatgatgcgcaagattttatgtggggggacagttgtgcatcagatttattttactattagatttgtgatttattagataacgattttgaattttttgaaattaactacagATA
TTCAAATGTTGAAATATTTTATGTTGAAAGATGCATCTCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCAC

Intron 4 GTTAATTTCA ... CTCTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	25	37
A2	100	0	24	33
A3	85.71	7	45	39
A4	82.86	6	29	29
A5	100	0	16	33
A6	89.7	17	144	152
A7	88.84	28	196	250
A8	93.35	15	188	233
A9	91.53	18	170	219
A10	92.59	14	154	196

>TRINITY_DN41149_c1_g1_i1 from sample A9 / Example spliced isoform

TTGGGGATATTTTGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCA
CAGGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAG

>TRINITY_DN41149_c1_g1_i2 from sample A9 / Example unspliced isoform:

TTGGGGATATTTTGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCA
CAGgttaatttcaatcgtttcaatcggctatcctgcaattatataagccgatcggcgtatatcacaggtttaatcctagcctcttgccaggtgggagcttcaacctgttaaattaatcggagcctcttgccagGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAG

Intron 5 GTGAGAGCTT ... TCTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.7	551	66.5	582.8
A2	50.22	538	64.7	525.8
A3	47.28	597.5	71.8	532.2
A4	51.18	450.3	70.8	451
A5	47.48	325.7	45	291.3
A6	51.68	3752.2	336.3	3821.2
A7	48.8	4114.5	420	3829
A8	48.79	3523	371.5	3279.2
A9	47.25	3558.7	354	3158.5
A10	48.76	3177	296.5	2955.5

>TRINITY_DN124100_c2_g1_i7 from sample A3 / Example spliced isoform

TGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCACAGGTTAATTTC
AATCGTTTCAATCGGCTATCCTGCAATTATATAAGCCGATCGGCGTATATCACAGGTTTAATCCTAGCCTCTTGCCAGGTGGGAGCTTCAACCTGTTAAATTAATCGGAGCCTCTTGCCAGGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAGGCTAATGGTGGAGATAACGTGCA
TCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGCAACCGA

>TRINITY_DN124100_c2_g1_i6 from sample A3 / Example unspliced isoform:

TGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCACAGGTTAATTTC
AATCGTTTCAATCGGCTATCCTGCAATTATATAAGCCGATCGGCGTATATCACAGGTTTAATCCTAGCCTCTTGCCAGGTGGGAGCTTCAACCTGTTAAATTAATCGGAGCCTCTTGCCAGgtgagagcttcaatgggttaatctgtagtggaggaggaccgtggttaaatgaatggtcctcgtttcagtttcagtttca
agggaatacctaatatattaggttactccgtgtctttcaatttcttttgaggttgtttgttttcttttgcagGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAGGCTAATGGTGGAGATAACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAA
GTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGCAACCGA

Intron 6 GTTTCTTCCT ... TTAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.04	46	726	89
A2	94.74	38	685	84
A3	94.76	40	724	64
A4	97.18	14	482	64
A5	94.74	20	360	34
A6	99.14	69	7922	32
A7	97.53	167	6597	78
A8	98.87	94	8219	44
A9	98.77	81	6513	54
A10	97.74	110	4759	72

>TRINITY_DN124100_c2_g1_i1 from sample A3 / Example spliced isoform

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGATATTCAAATGTTGAAAGATGCATATCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCACGTTATGAAAGATGCATCGATAATACATGAAACATCTCACGTTATGAAAGATGCATCGATTG
AAGTTCTACTGCAGTATTATGGGCGCACTACAATTATGGTTTTATTTCATTCAATGTTGCAGG

>TRINITY_DN124100_c2_g1_i12 from sample A3 / Example unspliced isoform:

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGgtttcttcctatcgccaacattagccgcatcatgaagataaagtttctgcatcttcttcattttcttgactatagtttcgttatatcttggaccctgcatggccatcttcactggcttgaatttctttg
gcagcctaaaccattcatgaagctaatctctatttcttggtccggttcactcgatttcttgatttttttaaataaactttcaataataatgatgcgcaagattttatgtggggggacagttgtgcatcagatttattttactattagatttgtgatttattagataacgattttgaattttttgaaattaactacagATA
TTCAAATGTTGAAATATTTTATGTTGAAAGATGCATCTCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCAC

Intron 7 GTAATTATTA ... GATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	1	0
A2	100	0	2	2
A3	71.43	1	2	3
A4	50	2	3	1
A5	-1	0	0	0
A6	72.22	5	14	12
A7	51.58	23	29	20
A8	77.14	4	14	13
A9	75	7	22	20
A10	74.55	7	18	23

>TRINITY_DN49227_c4_g1_i18 from sample A7 / Example spliced isoform

TCCAACGGTGCACTTCCTTCTCCTCCAAATATTGCAGGAATTGTGTTTGCATTCGCTTTTTCACCGGGAATTGTTGAGCAACCCAGTTGATCCAATAAATCAGACGCAGCGGCAGAATTGATGTTGTACATATAATTGTCGTGCGCCAAAACTGGAACCTGTCCGGCTCCACCGTGACACTCGTAAATCTCCAACGGTGC
ACTTCCTTCTCCTCCAAATATTGCAGGAATTGTGTTTGCATTCGCTTTTTCACCGGGAATTGTTGAGCAACCCAGTTGATCCAATAAATCAGACGCAGCGGCAGAATTGATGTTGTACATATAATTGTCGTGCGCCAAAACTGGAACCTGTCCGGCTCCACCGTGACACTCGTAAATCTCCAACGGTGCACTTCCTTCTC
CAGATATTGCAGGAATTGCCTTTGCATTCGCTTCTTCACCGGGAATTCTTGAGCAACCCACTTGATCACCAACCACTCCTCCTCCCTCCATCTGGATGTGATAACACTGATGCCAGCTGCTGTTAATATCGAAATACATGGGGAGACCCTGCAAGTTAACGTCATATATCAAATTCAGAGAATCCTTCCAACCATCCAGA
GGATCGAGACTGTTAGACTGGCCAGCGGTCCATCTAATTGATGGCACACTAGCTTGGGCCAGGGAATTCCATGGCGGGCATAACTGATGGGTTTTCTTGTCATGATCCACACTTAGGAGATCCGGCGGTATTCTATTTCCCAACATCTCATTGGTATAAGAATCTCCTGAATAGACATCTGTGAAATCAGCAGCAATATT
TGAATAGGAATGACCAGCGCTCAATGCACTTCTTCCAGAAGATTCTGTGAACGACGAATAGTTGATCATGATCTCGTTTTCACTTATCTGGCCATTCTTCTCAAATTTTCTTTCAAGGGTTGTCTTTCAACAAGATCAGGGATGGTGATTACGGTTGAGCCCAGAAGGCTTATGCAGGCCTTCCTCTTGCTGAGTCGGAG
ATATAAAAGCTGTATGAGTAGAGAATTTCTCATGTGTATGGGTAGTATCAGTATTAGTCGACAGCTGAGAATAAGAACTTAGCTGGTTTTCGATGGGTTCTTGATCAATTAAAAACTCATTATTAGGATACTGTGCTTGTTCTTGTTTTAATTCCAACCCACCCAGAACACACAACTGATTTTCTTGAGTGGCGAGTAGA
ATATTACAGGTGCTGTTATGTGTCTGATATCCTTCTTCTGGCATGACTTCCAAGCACCGCTGGGCAGAGTAAGACGCCGGAGCAACATCATGACCATCAACAGGCACTGAATTAGCATTGAAAATACGATCGGGTTCTGAAGGGATATTAAAATTGCTGTTGGTATTCCCGTCGTTGGCGGTGGAGGAGGATGGGCTGTG
GTTTTTCAAATTGAGCTTCTTCTTGATGCAAGAGTTCCAGTAATTCTTGATTTCGGTGTCCGTGCGTCCCGGAAGTTGTGCAGCGATTTAAGACCACCTGCTTATTATTGTCATCGACGTTAATTACGTCGATAGGCGTATTCTTTTAACTTTTGGGGTGGGTCTCGGGTGGGTGATTTATTGCATTTTTAATATTCAAC
GGTTAGATTTTAATGATAGAAGAATATGCTTCGTCTAGTGCATTGATGAAGCACTCAGATTTGCTGTGTTAACTCATGGACAGCTTTAAATTCAAACGACACATATTGTTTAAAATAATTTATTTACAAATGCTGTATGCCTGCCACTTCCCACTCACCTCCACTCCGCGCTGTACCCTCGCTGTACCCCCGCTCGACAC
CATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGGTCCAGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAGGCTAATGGTGGAGATAACGTGCATCATCG

>TRINITY_DN49227_c4_g1_i1 from sample A7 / Example unspliced isoform:

TCCAACGGTGCACTTCCTTCTCCTCCAAATATTGCAGGAATTGTGTTTGCATTCGCTTTTTCACCGGGAATTGTTGAGCAACCCAGTTGATCCAATAAATCAGACGCAGCGGCAGAATTGATGTTGTACATATAATTGTCGTGCGCCAAAACTGGAACCTGTCCGGCTCCACCGTGACACTCGTAAATCTCCAACGGTGC
ACTTCCTTCTCCTCCAAATATTGCAGGAATTGTGTTTGCATTCGCTTTTTCACCGGGAATTGTTGAGCAACCCAGTTGATCCAATAAATCAGACGCAGCGGCAGAATTGATGTTGTACATATAATTGTCGTGCGCCAAAACTGGAACCTGTCCGGCTCCACCGTGACACTCGTAAATCTCCAACGGTGCACTTCCTTCTC
CAGATATTGCAGGAATTGCCTTTGCATTCGCTTCTTCACCGGGAATTCTTGAGCAACCCACTTGATCACCAACCACTCCTCCTCCCTCCATCTGGATGTGATAACACTGATGCCAGCTGCTGTTAATATCGAAATACATGGGGAGACCCTGCAAGTTAACGTCATATATCAAATTCAGAGAATCCTTCCAACCATCCAGA
GGATCGAGACTGTTAGACTGGCCAGCGGTCCATCTAATTGATGGCACACTAGCTTGGGCCAGGGAATTCCATGGCGGGCATAACTGATGGGTTTTCTTGTCATGATCCACACTTAGGAGATCCGGCGGTATTCTATTTCCCAACATCTCATTGGTATAAGAATCTCCTGAATAGACATCTGTGAAATCAGCAGCAATATT
TGAATAGGAATGACCAGCGCTCAATGCACTTCTTCCAGAAGATTCTGTGAACGACGAATAGTTGATCATGATCTCGTTTTCACTTATCTGGCCATTCTTCTCAAATTTTCTTTCAAGGGTTGTCTTTCAACAAGATCAGGGATGGTGATTACGGTTGAGCCCAGAAGgtaattattaacagagccaggcgatactgtagt
cctgcatgacactatttttaattcatcgcatgtcgatgattttgcagGCTTATGCAGGCCTTCCTCTTGCTGAGTCGGAGATATAAAAGCTGTATGAGTAGAGAATTTCTCATGTGTATGGGTAGTATCAGTATTAGTCGACAGCTGAGAATAAGAACTTAGCTGGTTTTCGATGGGTTCTTGATCAATTAAAAACTCAT
TATTAGGATACTGTGCTTGTTCTTGTTTTAATTCCAACCCACCCAGAACACACAACTGATTTTCTTGAGTGGCGAGTAGAATATTACAGGTGCTGTTATGTGTCTGATATCCTTCTTCTGGCATGACTTCCAAGCACCGCTGGGCAGAGTAAGACGCCGGAGCAACATCATGACCATCAACAGGCACTGAATTAGCATTG
AAAATACGATCGGGTTCTGAAGGGATATTAAAATTGCTGTTGGTATTCCCGTCGTTGGCGGTGGAGGAGGATGGGCTGTGGTTTTTCAAATTGAGCTTCTTCTTGATGCAAGAGTTCCAGTAATTCTTGATTTCGGTGTCCGTGCGTCCCGGAAGTTGTGCAGCGATTTAAGACCACCTGCTTATTATTGTCATCGACGT
TAATTACGTCGATAGGCGTATTCTTTTAACTTTTGGGGTGGGTCTCGGGTGGGTGATTTATTGCATTTTTAATATTCAACGGTTAGATTTTAATGATAGAAGAATATGCTTCGTCTAGTGCATTGATGAAGCACTCAGATTTGCTGTGTTAACTCATGGACAGCTTTAAATTCAAACGACACATATTGTTTAAAATAATT
TATTTACAAATGCTGTATGCCTGCCACTTCCCACTCACCTCCACTCCGCGCTGTACCCTCGCTGTACCCCCGCTCGACACCATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGGTCCAGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAGG
CTAATGGTGGAGATAACGTGCATCATCG

Intron 8 GTGGGAGCTT ... CTCTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	3	4
A2	100	0	5	5
A3	74.55	7	24	17
A4	45.45	6	8	2
A5	100	0	9	9
A6	65.31	17	38	26
A7	73.95	28	90	69
A8	77.78	15	55	50
A9	72.31	18	54	40
A10	79.56	14	57	52

>TRINITY_DN41149_c1_g1_i1 from sample A9 / Example spliced isoform

TTGGGGATATTTTGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCA
CAGGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAG

>TRINITY_DN41149_c1_g1_i3 from sample A9 / Example unspliced isoform:

TTGGGGATATTTTGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCA
CAGgtgggagcttcaacctgttaaattaatcggagcctcttgccagGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAG

Intron 9 GTTAATTTCA ... CTCTTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.98	52.1	25	95.3
A2	66.36	42.3	24	83.6
A3	53.45	80	45	98.8
A4	61.91	45.1	29	73.1
A5	61.51	35.1	16	59.9
A6	62.51	250.1	144	412.6
A7	59.36	367.5	196	587.6
A8	62.77	318.5	188	557
A9	63.01	281.4	170	505.4
A10	61.06	272.5	154	461.6

>TRINITY_DN124100_c2_g1_i9 from sample A3 / Example spliced isoform

TGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCACAGGTGGGAGCT
TCAACCTGTTAAATTAATCGGAGCCTCTTGCCAGGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAGGCTAATGGTGGAGATAACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACC
ATACCGTGGTGGCAACCGA

>TRINITY_DN124100_c2_g1_i6 from sample A3 / Example unspliced isoform:

TGGGTTTTTATTTGTGGTATGATGCTGGGTAAGCGCTGCTTGTAACGGCCATTGTAATGGCCACGTTTTGTGGCCATACAAAACGATTGGAACGCCGGAGTAACCATGAAGGAATAGCCTATCTCTACCTGTGCTGTGGCCATTGCATTAAAATTTTAACCCTGTCATGGCGGTGGGGGAAGCGATCACAGgttaatttc
aatcgtttcaatcggctatcctgcaattatataagccgatcggcgtatatcacaggtttaatcctagcctcttgccagGTGGGAGCTTCAACCTGTTAAATTAATCGGAGCCTCTTGCCAGGTGAGAGCTTCAATGGGTTAATCTGTAGTGGAGGAGGACCGTGGTTAAATGAATGGTCCTCGTTTCAGTTTCAGTTTCA
AGGGAATACCTAATATATTAGGTTACTCCGTGTCTTTCAATTTCTTTTGAGGTTGTTTGTTTTCTTTTGCAGGTCCCGGAAAAGGAGGTTCCGGCTCCACACCAGATTCAGACGGAACCGAATTCAAGGCTAATGGTGGAGATAACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAA
GTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGCAACCGA

Intron 10 GTAATCCTCC ... CTCTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	92.17	69.7	206	820
A2	91.68	74.3	201	819
A3	91.94	79.3	211	905
A4	93.18	50.3	157	687
A5	88.05	65	112	479
A6	91.13	539	984	5535
A7	90.86	628	1638	6244
A8	89.69	608	1279	5290
A9	86.32	861	1412	5433
A10	88.89	614.3	1318	4916

>TRINITY_DN49227_c4_g1_i17 from sample A7 / Example spliced isoform

GCAGCGATTTAAGACCACCTGCTTATTATTGTCATCGACGTTAATTACGTCGATAGGCGTATTCTTTTAACTTTTGGGGTGGGTCTCGGGTGGGTGATTTATTGCATTTTTAATATTCAACGGTTAGATTTTAATGATAGAAGAATATGCTTCGTCTAGTGCATTGATGAAGCACTCAGATTTGCTGTGTTAACTCATGG
ACAGCTTTAAATTCAAACGACACATATTGTTTAAAATAATTTATTTACAAATGCTGTATGCCTGCCACTTCCCACTCACCTCCACTCCGCGCTGTACCCCCGCTCGACACCATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGGTCCAGGAAAAGGAGGTTCCGGCTCCA
CACCAGATTCAGACGGAACCGAATTCAAGGCTAATGGTGGAGATAACGTGCATCATCG

>TRINITY_DN49227_c4_g1_i4 from sample A7 / Example unspliced isoform:

TCCAACGGTGCACTTCCTTCTCCTCCAAATATTGCAGGAATTGTGTTTGCATTCGCTTTTTCACCGGGAATTGTTGAGCAACCCAGTTGATCCAATAAATCAGACGCAGCGGCAGAATTGATGTTGTACATATAATTGTCGTGCGCCAAAACTGGAACCTGTCCGGCTCCACCGTGACACTCGTAAATCTCCAACGGTGC
ACTTCCTTCTCCTCCAAATATTGCAGGAATTGTGTTTGCATTCGCTTTTTCACCGGGAATTGTTGAGCAACCCAGTTGATCCAATAAATCAGACGCAGCGGCAGAATTGATGTTGTACATATAATTGTCGTGCGCCAAAACTGGAACCTGTCCGGCTCCACCGTGACACTCGTAAATCTCCAACGGTGCACTTCCTTCTC
CAGATATTGCAGGAATTGCCTTTGCATTCGCTTCTTCACCGGGAATTCTTGAGCAACCCACTTGATCACCAACCACTCCTCCTCCCTCCATCTGGATGTGATAACACTGATGCCAGCTGCTGTTAATATCGAAATACATGGGGAGACCCTGCAAGTTAACGTCATATATCAAATTCAGAGAATCCTTCCAACCATCCAGA
GGATCGAGACTGTTAGACTGGCCAGCGGTCCATCTAATTGATGGCACACTAGCTTGGGCCAGGGAATTCCATGGCGGGCATAACTGATGGGTTTTCTTGTCATGATCCACACTTAGGAGATCCGGCGGTATTCTATTTCCCAACATCTCATTGGTATAAGAATCTCCTGAATAGACATCTGTGAAATCAGCAGCAATATT
TGAATAGGAATGACCAGCGCTCAATGCACTTCTTCCAGAAGATTCTGTGAACGACGAATAGTTGATCATGATCTCGTTTTCACTTATCTGGCCATTCTTCTCAAATTTTCTTTCAAGGGTTGTCTTTCAACAAGATCAGGGATGGTGATTACGGTTGAGCCCAGAAGGTAATTATTAACAGAGCCAGGCGATACTGTAGT
CCTGCATGACACTATTTTTAATTCATCGCATGTCGATGATTTTGCAGGCTTATGCAGGCCTTCCTCTTGCTGAGTCGGAGATATAAAAGCTGTATGAGTAGAGAATTTCTCATGTGTATGGGTAGTATCAGTATTAGTCGACAGCTGAGAATAAGAACTTAGCTGGTTTTCGATGGGTTCTTGATCAATTAAAAACTCAT
TATTAGGATACTGTGCTTGTTCTTGTTTTAATTCCAACCCACCCAGAACACACAACTGATTTTCTTGAGTGGCGAGTAGAATATTACAGGTGCTGTTATGTGTCTGATATCCTTCTTCTGGCATGACTTCCAAGCACCGCTGGGCAGAGTAAGACGCCGGAGCAACATCATGACCATCAACAGGCACTGAATTAGCATTG
AAAATACGATCGGGTTCTGAAGGGATATTAAAATTGCTGTTGGTATTCCCGTCGTTGGCGGTGGAGGAGGATGGGCTGTGGTTTTTCAAATTGAGCTTCTTCTTGATGCAAGAGTTCCAGTAATTCTTGATTTCGGTGTCCGTGCGTCCCGGAAGTTGTGCAGCGATTTAAGACCACCTGCTTATTATTGTCATCGACGT
TAATTACGTCGATAGGCGTATTCTTTTAACTTTTGGGGTGGGTCTCGGGTGGGTGATTTATTGCATTTTTAATATTCAACGGTTAGATTTTAATGATAGAAGAATATGCTTCGTCTAGTGCATTGATGAAGCACTCAGATTTGCTGTGTTAACTCATGGACAGCTTTAAATTCAAACGACACATATTGTTTAAAATAATT
TATTTACAAATGCTGTATGCCTGCCACTTCCCACTGCTGGAATTAAACCATCGTCGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGgtaatcctccattttttttgtttaatcctagcctcttgccaggtgggagcttcaacctgttaaattaatcggagcctctttccagGTCCAGGAA
AAGGAGGTTCCGGCTCCACACCAGATTCAGATGGAACCGAATTCAAGGCTAATGGTGGAGATCACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGATAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGCAACCGA

Intron 11 GTCCGGTTCA ... TTAACTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.6	227	633	207
A2	71.19	223	551	162
A3	71.16	242	597	146
A4	70.7	143	345	123
A5	71.05	119	292	78
A6	75.78	3165	9902	64
A7	75.84	2378	7466	123
A8	77.49	3422	11779	82
A9	77.92	2526	8916	99
A10	74.76	1439	4263	139

>TRINITY_DN124100_c2_g1_i17 from sample A3 / Example spliced isoform

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGGTTTCTTCCTATCGCCAACATTAGCCGCATCATGAAGATAAAGTTTCTGCATCTTCTTCATTTTCTTGACTATAGTTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCTTTG
GCAGCCTAAACCATTCATGAAGCTAATCTCTATTTCTTGATATTCAAATGTTGAAAGATGCATATCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCACGTTATGAAAGATGCATCGATAATACATGAAACATCTCACGTTATGAAAGATGCATCGATTGAAGTTCTACTGCAGTATTATGGGCGCACTACA
ATTATGGTTTTATTTCATTCAATGTTGCAGG

>TRINITY_DN124100_c2_g1_i14 from sample A3 / Example unspliced isoform:

GACGGCCAAGAAATCGAAAACCCAAAATCCGCAGAACAGATCCCCCAGTCCAGTGTGCAGGAGCAGGACAGGTTTCTTCCTATCGCCAACATTAGCCGCATCATGAAGATAAAGTTTCTGCATCTTCTTCATTTTCTTGACTATAGTTTCGTTATATCTTGGACCCTGCATGGCCATCTTCACTGGCTTGAATTTCTTTG
GCAGCCTAAACCATTCATGAAGCTAATCTCTATTTCTTGgtccggttcactcgatttcttgatttttttaaataaactttcaataataatgatgcgcaagattttatgggggggacagttgttttcaaaatatttgttaataaaaaatatttcagatttattttactattagatttgtgatttattagataacgattttg
aattttttgaaattaactacagATATTCAAATGTTGAAATATTTTATGTTGAAAGATGCATCTCAGAACACTCAAAACACTAAACCAATACATGAAACATCTCACGTTATGAAAGATGCATCGATAATACATGAAACATCTCACGTTATGAAAGATGCATCGATTGAAGTTCTACTGCAGTATTATGGGCGCACTACAAT
TATGGTTTTATTTCATTCAATGTTGCAGGTATGCTGCCTGTAATGTTCTCTTTTTGCTTTCAAAGAAT

Intron 12 GTAATCCTCC ... TCTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.23	468	125	680
A2	56.04	491	96	626
A3	54.82	511	124	620
A4	59.95	350	72	524
A5	52.24	320	58	350
A6	53.38	4054	476	4642
A7	52.71	4076	843	4543
A8	52.29	3572	724	3915
A9	49.62	3861	751	3802
A10	51.65	3323	703	3550

>TRINITY_DN45537_c0_g1_i11 from sample A6 / Example spliced isoform

ACTCACTCCTGTACGTGTTGTATGCCTGCCACTTCCCACTCTGGAATTAAACCATCATCGCTCACCTCCACTCCGCGATGTACCCCGCTCAACACCATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGGTCCACGAAAAGGAGGTTCCGACTCCACACCAGATTCAGATG
GAACCGATTTCAAGGCTAATGGTGGAGACAACGTGCATCATCGTTTAAAGCATTAAGGCATTCTACAATGACAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGAAACCGATGCTATCTTGTGGCGTGTGGCAACAGTTTAAAGTTCCTCCTTGAACCTTATATGAAG
CTCCCATGGCGGACGCTAACATCCTAGACGGCCAAGAAATCGAAAACCCCAAATCCGCCGAACAGATCCCCCAGTCCAGTGTGCAGGAGCA

>TRINITY_DN45537_c0_g1_i5 from sample A6 / Example unspliced isoform:

ACACCATAATTGTAACGCTCAATGGCCGAGATTTTATGTGGGCAGAAGTATACCGAAACGACAATGGgtaatcctccattttttttgtttaatcctagcctcttgctaggtgggagcttcaacctgttaaattaatcggagcctcttgccaggtgagagcttcaatgggttaatctgtagtggaggaggaccgccgttaa
atgaatggtcctcgtttcagtttcagtttcaagggaatacctaatatattaggttactccgtgtctttcaatttcttttgaggttgtttgttttcttttgcagGTCCACGAAAAGGAGGTTCCGACTCCACACCAGATTCAGATGGAACCGATTTCAAGGCTAATGGTGGAGACAACGTGCATCATCGTTTAAAGCATTA
AGGCATTCTACAATGACAGAGGCCAGTGAAAGTTCATTCGGACGATGCTGTGATTGCATGGGAGGTACCATACCGTGGTGGAAACCGATGCTATCTTGTGGCGTGTGGCAACAGTTTAAAGTTCCTCCTTGAACCTTATATGAAGCTCCCATGGCGGACGCTAACATCCTAGACGGCCAAGAAATCGAAAACCCCAAATC
CGCCGAACAGATCCCCCAGTCCAGTGTGCAGGAGCA