sp|Q8GWE6|EER5_ARATH ----> Enhanced ethylene response protein 5

Intron 1 GTAAGAACGG ... CTTATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.14	11	0	7
A2	47.37	5	5	4
A3	43.75	9	6	8
A4	26.32	7	4	1
A5	73.33	2	7	4
A6	23.08	5	2	1
A7	18.18	9	2	2
A8	37.93	9	5	6
A9	25	12	3	5
A10	25	6	2	2

>TRINITY_DN120419_c0_g1_i1 from sample A3 / Example spliced isoform

GGCGAAGCGGGAAGCGAGACCAGTACCCAATATATTAGAAAACACATCCCAATTGCGGCACACATATTGGTCTGAAATAGCGGAAGCGGAGGAGGAGAGAAATTGTTCTGAACACTAAAATTTGTAAAAATGACTGTTTATATGAGTATGGGAGAAGCTCATAGGAGACTTTCAGAATATGTGAATGAATTTACAGAGGC
CATTGCTTCTCAGAACGGCTCCGCATTGCAGCCACTACTTGCTATTTCCTCAAATTCACCTTATCGAGAGTCCGTGGCCTCGGCCATCCAAGTTTTCAAGGACATAAGCAGGGTGGTTAAGCAGGTGGATAAGTATGCACAACTTGGTGACATGCTTCAGCAACACATCCGTTGCATGCAGAGTTTTCAATCTCAGCACT
ATGTAGATGCTTATTATGCTTTAGAAAAGTCTGCAAATGCCTTTCTACAGGAGTTCCGAAATTGGGAAACAGCATGGGCAGTCGATGCTCTTTATACAATTGTGTATGAAATGCGGACACTTGCAGAGATGGCAGATAGAGAAATGGAAACAAATGGGAAAAACCCCGACAAACTTAAAGGAGCTGGGTCTTTCTTGATG
AAAGTTTTTGGATCGCTGGTGGGAAAAGGCCCCAAAAGACTTGGAGCATTGTATGTAACGTGTCAACTATTTAAGGTGTACTTTAAGCTGGGAACTGTCCATCTTTGCAGAAGTGTTATAAGGAGTATTGAGACAGCACGTGTTTTTGAATTTGAGGAATTTCCCACTAGGGATAAGGTTACCTATATGTACTACACAGG
GCGCTTAGAGGTGTTCAATGACAACTTTCTTGCAGCAGATCAGAAACTCATGTATGCATTAGAGCACTGCGATCCTCGAAAAGAAGCAAACATAAGGATGATTCTGAAGTACCTTATACCTGTTAAACTTTCGCTTGGGATATTACCAAAAGAATGGCTTCTTCAGAAGTATAATCTTCTTGAGTATATTGATGTTGTGC
GAGCTTTGCGTA

>TRINITY_DN120419_c0_g1_i2 from sample A3 / Example unspliced isoform:

GGCGAAGCGGGAAGCGAGACCAGTACCCAATATATTAGAAAACACATCCCAATTGCGGCACACATATTGGTCTGAAATAGCGGAAGCGGAGGAGGAGAGAAATTGTTCTGAACACTAAAATTTGTAAAAATGACTGTTTATATGAGTATGGGAGAAGCTCATAGGAGACTTTCAGAATATGTGAATGAATTTACAGAGGC
CATTGCTTCTCAGAACGGCTCCGCATTGCAGCCACTACTTGCTATTTCCTCAAATTCACCTTATCGAGAGTCCGTGGCCTCGGCCATCCAAGTTTTCAAGGACATAAGCAGGGTGGTTAAGCAGGTGGATAAGTATGCACAACTTGGTGACATGCTTCAGCAACACATCCGTTGCATGCAGAGTTTTCAATCTCAGCACT
ATGTAGATGCTTATTATGCTTTAGAAAAGTCTGCAAATGCCTTTCTACAGGAGTTCCGAAATTGGGAAACAGCATGGGCAGTCGATGCTCTTTATACAATTGTGTATGAAATGCGGACACTTGCAGAGATGgtaagaacggaattgctatgtccaaattttttgctttggtttgacactagaaatgtttacttattgcag


GCAGATAGAGAAATGGAAACAAATGGGAAAAACCCCGACAAACTTAAAGGAGCTGGGTCTTTCTTGATGAAAGTTTTTGGATCGCTGGTGGGAAAAGGCCCCAAAAGACTTGGAGCATTGTATGTAACGTGTCAACTATTTAAGGTGTACTTTAAGCTGGGAACTGTCCATCTTTGCAGAAGTGTTATAAGGAGTATTGA
GACAGCACGTGTTTTTGAATTTGAGGAATTTCCCACTAGGGATAAGGTTACCTATATGTACTACACAGGGCGCTTAGAGGTGTTCAATGACAACTTTCTTGCAGCAGATCAGAAACTCATGTATGCATTAGAGCACTGCGATCCTCGAAAAGAAGCAAACATAAGGATGATTCTGAAGTACCTTATACCTGTTAAACTTT
CGCTTGGGATATTACCAAAAGAATGGCTTCTTCAGAAGTATAATCTTCTTGAGTATATTGATGTTGTGCGAGCTTTGCGTA

Intron 2 GTATCCTTAA ... TTAAATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.5	5	3	0
A2	44.12	19	15	2
A3	7.69	12	1	1
A4	23.81	16	5	1
A5	40	12	8	5
A6	12.5	7	1	1
A7	37.5	5	3	0
A8	9.09	10	1	0
A9	14.29	6	1	0
A10	24	19	6	0

>TRINITY_DN88976_c0_g1_i1 from sample A5 / Example spliced isoform

AACACTTTCCTTCGCTTAAATTATTACTCTTTTTATAAAATCACTTCGAACTTGCAGTTGCCCAATTGCAGTTGCCCAAGTAAATCACAGAGAACACCAAGGATCAAAACCTCCAACGAACTTAACTAACTCTAGAGCAGGCGAAGCGGGAAGCGAGACCAGTACCCAATATATTAGAAAACACATCCCAATTGCGGCAC
ACATATTGGTCTGAAATAGCGGAAGCGGAGGAGGAGAGAAATTGTTCTGAACACTAAAATTTGTAAAAATGACTGTTTATATGAGTATGGGAGAAGCTCATAGGAGACTTTCAGAATATGTGAATGAATTTACAGAGGCCATTGCTTCTCAGAACGGCTCCGCATTGCAGCCACTACTTGCTATTTCCTCAAATTCACCC
TATCGAGAGTCCGTGGCCTCGGCCATCCAAGTTTTCAAGGACATAAGCAGGGTGGTTAAGCAGGTGGATAAGTATGCACAACTTGGTGACATGCTTCAGCAACACATCCGTTGCATGCAGAGTTTTCAATCTCAGCACTATGTAGATGCTTATTATGCTTTAGAAAAGTCTGCAAATGCCTTTCTACAGGAGTTCCGAAA
TTGGGAAACAGCATGGGCAGTCGATGCTCTTTATACAATTGTGTATGAAATGCGGACACTTGCAGAGATGGTAAGAACGGAATTGCTATGTCCAAATTTTTTGCTTTGGTTTGACACTAGAAATGTTTACTTATTGCAGGCAGATAGAGAAATGGAAACAAATGGGAAAAACCCCGACAAACTTAAAGGAGCTGGGTCTT
TCTTGATGAAAGTTTTTGGATCGCTGGTGGGAA

>TRINITY_DN88976_c0_g1_i2 from sample A5 / Example unspliced isoform:

AACACTTTCCTTCGCTTAAATTATTACTCTTTTTATAAAATCACTTCGAACTTGCAGTTGCCCAATTGCAGTTGCCCAAGTAAATCACAGAGAACACCAAGGATCAAAACCTCCAACGAACTTAACTAACTCTAGAGCAGGCGAAGCGGGAAGCGAGACCAGTACCCAATATATTAGAAAACACATCCCAATTGCGGCAC
ACATATTGGTCTGAAATAGCGGAAGCGGAGGAGGAGAGAAATTGTTCTGAACACTAAAATTTGTAAAAATGACTGTTTATATGAGTATGGGAGAAGCTCATAGGAGACTTTCAGAATATGTGAATGAATTTACAGAGGCCATTGCTTCTCAGAACGGCTCCGCATTGCAGCCACTACTTGCTATTTCCTCAAATTCACCC
TATCGAGAGTCCGTGGCCTCGGCCATCCAAGTTTTCAAGGACATAAGCAGGGTGGTTAAGCAGGTGGATAAGTATGCACAACTTGGTGACATGCTTCAGCAACACATCCGTTGCATGCAGAGTTTTCAATCTCAGCACTATGTAGATGCTTATTATGCTTTAGAAAAGTCTGCAAAgtatccttaactacatgtttagat
cttctatttgtaacatcatgcggtaatcttcagtggtacaggtacatcataattaaagaacacacccttaactttaaatacagTGCCTTTCTACAGGAGTTCCGAAATTGGGAAACAGCATGGGCAGTCGATGCTCTTTATACAATTGTGTATGAAATGCGGACACTTGCAGAGATGGTAAGAACGGAATTGCTATGTCC
AAATTTTTTGCTTTGGTTTGACACTAGAAATGTTTACTTATTGCAGGCAGATAGAGAAATGGAAACAAATGGGAAAAACCCCGACAAACTTAAAGGAGCTGGGTCTTTCTTGATGAAAGTTTTTGGATCGCTGGTGGGAA