sp|Q39183|D6KL2_ARATH ----> Serine/threonine-protein kinase D6PKL2

Intron 1 GTACACTGTA ... TGACCTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.83	17	0	82
A2	76.92	21	2	70
A3	72.87	35	0	94
A4	80.72	16	1	67
A5	77.78	14	2	49
A6	83.8	23	1	119
A7	79.46	53	0	205
A8	79.07	18	0	68
A9	79.73	45	2	177
A10	76.47	44	0	143

>TRINITY_DN101060_c0_g1_i2 from sample A4 / Example spliced isoform

ACGGATTCCAAACCTTTCTGAATTGCAGTTTGGAGAATACTTCGTCTGTTCAAGGATGCGGTGGATCTGCTACTGGAAGAGCTTCTAGCAGAGAGAAAATGTACCAGATCGAAAATTTAAGCTCGGATCGCGTGATCATCGCCTCCGGCATTTTCCCTTAGCGGAAGCTTGTTGCAGATTTCGGCTTGCGTCTGGAGCAT
TTTTACGTTTCTTCGCTGGTAGCTTGATAAGGTTATTCGTTTTACTTAAGTGGCACGTATTGAATCGTGGGTTTGCTAGAAGGAGCATACTCAGAATTGGGTTTCACTTGATTTAGGCGCATTTGCAAGATTAAGAATCAGGCATGTTCTGGTAGTTTGTAATTGCATATCCAGATGGCCAGCCCGGACATGCAGAGAGC
AGACGAGTGCAATCAGAGTACGGTATCAACAGAATCAGCCAGAGAGAAGAGCACTGATTCTTGTGTTCCAATGAGTGGGACTCTGAGTACATGTAGTAGTATTGATGTAAGTTTCAAGAGCAGTCTGAGCAGTAACATCACCGGTTCTAGTAGCGTCAAGAGCAGTTTTAGTGGTAAGGTAAGTGACGGCATGGGTGCAG
ATACGAAGATTCCTGAATGTAAATCCGTTGAAAATTTCAAGAACGAGAGGCTTGAATGTGTGGAAAGTGGCAAGAGTAGCCTGAGCGTGGCCAGCTACAGAAGCAGTGCTAGTGACAGCAATGAAAGTAACTGCAGTAGTTTTAGCAGTGCTCTAAACAAACCACACAAAGGCAATGATCCCAGATGGGAAGCCATTCAG
GCTGTGAAAGTCAGAGATGGATTTTTGGGTTTGAGTCATTTCAGGCTCCTGAAGCGCTTGGGAAGTGGGGATATTGGGAGTGTTTACTTAGCAGAGTTGAGGGGAACCAACTGTTTTTTTGCAATGAAAGTAATGGATAAAGGGTCTCTGGAAAACAGGAATAAGTTGCTCAGAGCGCAGACCGAGAGGGAGATTCTTTC
TTGTTTAGATCATCCTTTCCTACCGACCTTGTATTCTCATTTCGAGACAGACAAGTTCTCGTGCCTAGTCATGGAGTTTTGCAGTGGAGGGGATCTACATAGCTTTCGGCAGCGACAGACTGTGAAACGCTTTTCTGAAGCTGCTTCACGGTTCTATTCAGCAGAAATCTTACTTGCCCTGGAGTATTTGCACATGCTGG
GAATAGTCTACAGAGACCTGAAGCCTGAAAATGTTCTAGTTAGAGGGGATGGACACATTATGCTTCTAGACTTTGATTTATCTTTAAGGTGTGTTGTCAGTCCCACTCTGGTAAAGTCTGCATCTCCCAGTGTCGATCCTTCACGAAGAGTCCCTGTTTACTGTGTTCAACCCTCTTGCATAGAGCCTTCATGCGTGACC
CCAGTTTGTCTGCAGCCTTCTTGTTTCAGACCTCGTTTTTTTCCTCAGAGATCCAAGAAAACGCGAAAGCCAAAGAATGAGATGGGAAGTCAGGCAACCCTTCTTCCAGAACTCATTGCTGAGCCGACCAGTGCTCGTTCGATGTCGTTTGTGGGCACTCACGAGTACCTGGCTCCCGAAATAATCAAAGGGCAGGGCCA
CGGGAGTGCTGTCGATTGGTGGACATTTGGGATATTTTTATACGAGCTGCTATACGGGACGACGCCGTTCAAGGGAGCAGGCAATCGAGAAACATTAGAGAACGTTGTCGGGCAACCATTGAGGTTCCCAGATTCTCCTACTGTGAGTTTTGGTGCACGCGACTTGATCAGAGGTTTGCTTGTGAAAGACCCGCAACACA
GGCTAGGATTCAAAAGGGGAGCCACTGAAATCAAACAACATCCATTCTTTGATGGCGCAAACTTGGCTCTCATTCGCAGCATGACTCCTCCTCAAATCCCCAGACCCATTGATCTGAGCTTCTTAACGAACAAAGTCACTCCTCCCAGTGCTCGTGGTGAGAAGAAAATAGTAGATCCTGATGCTAATTCTTTAGATTTT
GATTTTTTCTAGTCTGGATAATGCCCTGCAATCCAATCTTCAGAAACTTGCTTATATTTGTTGCTCAATTTTCAAGGAATTTATAAGTTTGTATTGTAGGCTGTGCCTATGTTTCAGTAAAAACTTCAGTCCAACCTGGATTTTTTTGCAGAAGTGCCTCAAAAATCACAGTGGATTACGAGCTTCTTTTTAAGCTGTGT
ATCATACTTTTCCCTTACAAATTGGTTGCGATTAAACCATAAACTTCAGTTATCTGCAAATGTAGTTTCACTTATTTTTTAGCTTACATATAAATAAATGGAAGCCTACTAAAGTTTCAATAATGGAGAGGATCTCAGAGCTTATTATAAAGCTAATTATTGTCAATGGCTGTGAAAAAGGAGC

>TRINITY_DN101060_c0_g1_i1 from sample A4 / Example unspliced isoform:

ACGGATTCCAAACCTTTCTGAATTGCAGTTTGGAGAATACTTCGTCTGTTCAAGgtacactgtatagatttggtttttttgatttgttcattgggcattccgaaaaagcttagggggaggtcgtctaacccagcttcaatggcgttttcttgacctaaagGATGCGGTGGATCTGCTACTGGAAGAGCTTCTAGCAGAGA
GAAAATGTACCAGATCGAAAATTTAAGCTCGGATCGCGTGATCATCGCCTCCGGCATTTTCCCTTAGCGGAAGCTTGTTGCAGATTTCGGCTTGCGTCTGGAGCATTTTTACGTTTCTTCGCTGGTAGCTTGATAAGGTTATTCGTTTTACTTAAGTGGCACGTATTGAATCGTGGGTTTGCTAGAAGGAGCATACTCAG
AATTGGGTTTCACTTGATTTAGGCGCATTTGCAAGATTAAGAATCAGGCATGTTCTGGTAGTTTGTAATTGCATATCCAGATGGCCAGCCCGGACATGCAGAGAGCAGACGAGTGCAATCAGAGTACGGTATCAACAGAATCAGCCAGAGAGAAGAGCACTGATTCTTGTGTTCCAATGAGTGGGACTCTGAGTACATGT
AGTAGTATTGATGTAAGTTTCAAGAGCAGTCTGAGCAGTAACATCACCGGTTCTAGTAGCGTCAAGAGCAGTTTTAGTGGTAAGGTAAGTGACGGCATGGGTGCAGATACGAAGATTCCTGAATGTAAATCCGTTGAAAATTTCAAGAACGAGAGGCTTGAATGTGTGGAAAGTGGCAAGAGTAGCCTGAGCGTGGCCAG
CTACAGAAGCAGTGCTAGTGACAGCAATGAAAGTAACTGCAGTAGTTTTAGCAGTGCTCTAAACAAACCACACAAAGGCAATGATCCCAGATGGGAAGCCATTCAGGCTGTGAAAGTCAGAGATGGATTTTTGGGTTTGAGTCATTTCAGGCTCCTGAAGCGCTTGGGAAGTGGGGATATTGGGAGTGTTTACTTAGCAG
AGTTGAGGGGAACCAACTGTTTTTTTGCAATGAAAGTAATGGATAAAGGGTCTCTGGAAAACAGGAATAAGTTGCTCAGAGCGCAGACCGAGAGGGAGATTCTTTCTTGTTTAGATCATCCTTTCCTACCGACCTTGTATTCTCATTTCGAGACAGACAAGTTCTCGTGCCTAGTCATGGAGTTTTGCAGTGGAGGGGAT
CTACATAGCTTTCGGCAGCGACAGACTGTGAAACGCTTTTCTGAAGCTGCTTCACGGTTCTATTCAGCAGAAATCTTACTTGCCCTGGAGTATTTGCACATGCTGGGAATAGTCTACAGAGACCTGAAGCCTGAAAATGTTCTAGTTAGAGGGGATGGACACATTATGCTTCTAGACTTTGATTTATCTTTAAGGTGTGT
TGTCAGTCCCACTCTGGTAAAGTCTGCATCTCCCAGTGTCGATCCTTCACGAAGAGTCCCTGTTTACTGTGTTCAACCCTCTTGCATAGAGCCTTCATGCGTGACCCCAGTTTGTCTGCAGCCTTCTTGTTTCAGACCTCGTTTTTTTCCTCAGAGATCCAAGAAAACGCGAAAGCCAAAGAATGAGATGGGAAGTCAGG
CAACCCTTCTTCCAGAACTCATTGCTGAGCCGACCAGTGCTCGTTCGATGTCGTTTGTGGGCACTCACGAGTACCTGGCTCCCGAAATAATCAAAGGGCAGGGCCACGGGAGTGCTGTCGATTGGTGGACATTTGGGATATTTTTATACGAGCTGCTATACGGGACGACGCCGTTCAAGGGAGCAGGCAATCGAGAAACA
TTAGAGAACGTTGTCGGGCAACCATTGAGGTTCCCAGATTCTCCTACTGTGAGTTTTGGTGCACGCGACTTGATCAGAGGTTTGCTTGTGAAAGACCCGCAACACAGGCTAGGATTCAAAAGGGGAGCCACTGAAATCAAACAACATCCATTCTTTGATGGCGCAAACTTGGCTCTCATTCGCAGCATGACTCCTCCTCA
AATCCCCAGACCCATTGATCTGAGCTTCTTAACGAACAAAGTCACTCCTCCCAGTGCTCGTGGTGAGAAGAAAATAGTAGATCCTGATGCTAATTCTTTAGATTTTGATTTTTTCTAGTCTGGATAATGCCCTGCAATCCAATCTTCAGAAACTTGCTTATATTTGTTGCTCAATTTTCAAGGAATTTATAAGTTTGTAT
TGTAGGCTGTGCCTATGTTTCAGTAAAAACTTCAGTCCAACCTGGATTTTTTTGCAGAAGTGCCTCAAAAATCACAGTGGATTACGAGCTTCTTTTTAAGCTGTGTATCATACTTTTCCCTTACAAATTGGTTGCGATTAAACCATAAACTTCAGTTATCTGCAAATGTAGTTTCACTTATTTTTTAGCTTACATATAAA
TAAATGGAAGCCTACTAAAGTTTCAATAATGGAGAGGATCTCAGAGCTTATTATAAAGCTAATTATTGTCAATGGCTGTGAAAAAGGAGC

Intron 2 GTACAAGGTA ... TGACCTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.83	17	0	82
A2	76.92	21	2	70
A3	72.87	35	0	94
A4	80.72	16	0	67
A5	77.78	14	2	49
A6	83.22	24	1	119
A7	79.46	53	0	205
A8	79.07	18	0	68
A9	79.73	45	1	177
A10	76.47	44	0	143

>TRINITY_DN111470_c0_g1_i1 from sample A2 / Example spliced isoform

ACGGATTCCAAACCTTTCTGAATTGCAGTTTGGAGAATACTTGGTCTGTTCAAGGATGCGGTGGATCTGCTACTGGAAGAGCTTCTAGCAGAGAGAAAATGTACCAGATCGAAAATTTAAGCTCGGATCGCGTGATCATCGCCTCCGGCATTTTCCCTTAGCGGAAGCTTGTTGCAGATTTCGGCTTGCGTCTGGAGCAT
TTTTACGTTTCTTCGCTGGTAGCTTGATAAGGTTATTCGTTTTACTTAAGTGGCACGTATTGAATCGTGGGTTTGCTAGAAGGAGCATACTCAGAATTGGGTTTCACTTGATTTAGGCGCATTTGCAAGATTAAGAATCAGGCATGTTCTGGTAGTTTGTAATTGCATATCCAGATGGCCAGCCCGGACATGCAGAGAGC
AGACGAGTGCAATCAGAGTACGGTATCAACAGAATCAGCCAGAGAGAAGAGCACTGATTCTTGTGTTCCAATGAGTGGGACTCTGAGTACATGTAGTAGTATTGATGTAAGTTTCAAGAGCAGTCTGAGCAGTAACATCACCGGTTCTAGTAGCGTCAAGAGCAGTTTTAGTGGTAAGGTAAGTGACGGCATGGGTGCAG
ATACGAAGATTCCTGAATGTAAATCCGTTGAAAATTTCAAGAACGAGAGGCTTGAATGTGTGGAAAGTGGCAAGAGTAGCCTGAGCGTGGCCAGCTACAGAAGCAGTGCTAGTGACAGCAATGAAAGTAACTGCAGTAGTTTTAGCAGTGCTCTAAACAAACCACACAAAGGCAATGATCCCAGATGGGAAGCCATTCAG
GCTGTGAAAGTCAGAGATGGCTTTTTGGGTTTGAGTCATTTCAGGCTCCTGAAGCGCTTGGGAAGTGGGGATATTGGGAGTGTTTACTTAGCAGAGTTGAGGGGAACCAACTGTTTTTTTGCAATGAAAGTAATGGATAAAGGGTCTCTGGAAAACAGGAATAAGTTGCTCAGAGCGCAGACCGAGAGAGAGATTCTTTC
TTGTTTAGATCATCCTTTCCTACCGACCTTGTATTCTCATTTCGAGACAGACAAGTTCTCGTGCCTAGTCATGGAGTTTTGCAGTGGAGGGGATCTACATAGCTTTCGGCAGCGACAGACTGTGAAACGCTTTTCTGAAGCTGCTTCACGGTTCTATTCAGCAGAAATCTTACTCGCCCTGGAGTATTTGCACATGCTGG
GAATAGTCTATAGAGACCTGAAGCCTGAAAATGTTCTAGTTAGAGGGGATGGACACATTATGCTTCTAGACTTTGATTTATCTTTAAGGTGTGTTGTCAGTCCCACTCTGGTAAAGTCTGCATCTCCCAGTGTCGATCCTTCACGAAGAGTCCCTGTTTACTGTGTTCAACCCTCTTGCATAGAGCCTTCATGCGTGACC
CCAGTTTGTCTGCAGCCTTCTTGTTTCAGACCTCGTTTTTTTCCTCAGAGATCCAAGAAAACGCGAAAGCCAAAGAATGAGATGGGAAGTCAGGCAACCCTTCTTCCAGAACTCATTGCTGAGCCGACCAGTGCTCGTTCGATGTCGTTTGTGGGCACTCACGAGTACCTGGCTCCCGAAATAATCAAAGGGCAGGGCCA
CGGGAGTGCTGTCGATTGGTGGACATTTGGGATATTTTTATACGAGCTGCTATACGGGACGACGCCGTTCAAGGGAGCAGGCAATCGAGAAACATTAGAGAACGTTGTCGGGCAACCATTGAGGTTCCCAGATTCTCCTACTGTGAGTTTTGGTGCACGCGACTTGATCAGAGGTTTGCTTGTGAAAGACCCGCAACACA
GGCTAGGATTCAAAAGGGGAGCCACTGAAATCAAACAACATCCATTCTTTGATGGCGCAAACTTGGCTCTCATTCGCAGCATGACTCCTCCTCAAATCCCCAGACCCATTGATCTGAGCTTCTTAACGAACAAAGTCACTCCTCCCAGTGCTCGTGGTGAGAAGAAAATAGTAGATCCTGATGCTAATTCTTTAGATTTT
GATTTTTTCTAGTCTGGATAATGCCCTGCAATCCAATCTTCAGAAACTTGCTTATATTTGTTGCTCAATTTTCAAGGAATTTATAAGTTTGTATTGTAGGCTGTGCCTATGTTTCAGTAAAATCTTCAGTCCAACCTGGATTTTTTTGCAGAAGTGCCTCAAAAATCACAGTGGATTACGAGCTTCTTTTTAAGCTGTGT
ATCATACTTTTCCCTTACAAATTGGTTGCGATTAAACTATAAACTTCAGTTATCTGCAAATGTAGTTTCACTTATTTTTTAGCTTACATATAAATAAATGGAAGCCTACTAAAGTTTCAATAATGGAGAGGATCTCAGAGCTTATTATAAAGCTAATTATTGTCAATGGCTGTGAAAAAGGAGCAATAATTGTCAAATGT
GTCCCTGCTATTCTGTAACTTAGGTCACTTTTGGTGTTTATAATTGACGAATGTGGCCCTGCAATTCTGTAACATAGGTCACTTTTTGTGTTTTGTATGTTGTGGCCCCATGCAATTCTTGTAACTTGTGGTAAACTTTTGATGCTGGTTCGGAAAAGTAAGTACTTTATGATGACTTCCTGCTTTTCGGCATAGGCTGT
TTTAAATTTCTTTATAATGATGACATGCTGAGGTTGTTCTTGGTTTGATTATTGACCATTCTAATTTAGAATCGAC

>TRINITY_DN111470_c0_g1_i2 from sample A2 / Example unspliced isoform:

GAGAATACTTGGTCTGTTCAAGgtacaaggtacactgtatagatttggtttttttgatttgttcattgggcattccgaaaaagcttagggggaggtcgtctaacccagcttcaatggcgttttcttgacctaaagGATGCGGTGGATCTGCTACTGGAAGAGCTTCTAGCAGAGAGAAAATGTACCAGATCGAAAATTTA
AGCTCGGATCGCGTGATCATCGCCTCCGGCATTTTCCCTTAGCGGAAGCTTGTTGCAGATTTCGGCTTGCGTCTGGAGCATTTTTACGTTTCTTCGCTGGTAGCTTGATAAGGTTATTCGTTTTACTTAAGTGGCACGTATTGAATCGTGGGTTTGCTAGAAGGAGCATACTCAGAATTGGGTTTCACTTGATTTAGGCG
CATTTGCAAGATTAAGAATCAGGCATGTTCTGGTAGTTTGTAATTGCATATCCAGATGGCCAGCCCGGACATGCAGAGAGCAGACGAGTGCAATCAGAGTACGGTATCAACAGAATCAGCCAGAGAGAAGAGCACTGATTCTTGTGTTCCAATGAGTGGGACTCTGAGTACATGTAGTAGTATTGATGTAAGTTTCAAGA
GCAGTCTGAGCAGTAACATCACCGGTTCTAGTAGCGTCAAGAGCAGTTTTAGTGGTAAGGTAAGTGACGGCATGGGTGCAGATACGAAGATTCCTGAATGTAAATCCGTTGAAAATTTCAAGAACGAGAGGCTTGAATGTGTGGAAAGTGGCAAGAGTAGCCTGAGCGTGGCCAGCTACAGAAGCAGTGCTAGTGACAGC
AATGAAAGTAACTGCAGTAGTTTTAGCAGTGCTCTAAACAAACCACACAAAGGCAATGATCCCAGATGGGAAGCCATTCAGGCTGTGAAAGTCAGAGATGGCTTTTTGGGTTTGAGTCATTTCAGGCTCCTGAAGCGCTTGGGAAGTGGGGATATTGGGAGTGTTTACTTAGCAGAGTTGAGGGGAACCAACTGTTTTTT
TGCAATGAAAGTAATGGATAAAGGGTCTCTGGAAAACAGGAATAAGTTGCTCAGAGCGCAGACCGAGAGAGAGATTCTTTCTTGTTTAGATCATCCTTTCCTACCGACCTTGTATTCTCATTTCGAGACAGACAAGTTCTCGTGCCTAGTCATGGAGTTTTGCAGTGGAGGGGATCTACATAGCTTTCGGCAGCGACAGA
CTGTGAAACGCTTTTCTGAAGCTGCTTCACGGTTCTATTCAGCAGAAATCTTACTCGCCCTGGAGTATTTGCACATGCTGGGAATAGTCTATAGAGACCTGAAGCCTGAAAATGTTCTAGTTAGAGGGGATGGACACATTATGCTTCTAGACTTTGATTTATCTTTAAGGTGTGTTGTCAGTCCCACTCTGGTAAAGTCT
GCATCTCCCAGTGTCGATCCTTCACGAAGAGTCCCTGTTTACTGTGTTCAACCCTCTTGCATAGAGCCTTCATGCGTGACCCCAGTTTGTCTGCAGCCTTCTTGTTTCAGACCTCGTTTTTTTCCTCAGAGATCCAAGAAAACGCGAAAGCCAAAGAATGAGATGGGAAGTCAGGCAACCCTTCTTCCAGAACTCATTGC
TGAGCCGACCAGTGCTCGTTCGATGTCGTTTGTGGGCACTCACGAGTACCTGGCTCCCGAAATAATCAAAGGGCAGGGCCACGGGAGTGCTGTCGATTGGTGGACATTTGGGATATTTTTATACGAGCTGCTATACGGGACGACGCCGTTCAAGGGAGCAGGCAATCGAGAAACATTAGAGAACGTTGTCGGGCAACCAT
TGAGGTTCCCAGATTCTCCTACTGTGAGTTTTGGTGCACGCGACTTGATCAGAGGTTTGCTTGTGAAAGACCCGCAACACAGGCTAGGATTCAAAAGGGGAGCCACTGAAATCAAACAACATCCATTCTTTGATGGCGCAAACTTGGCTCTCATTCGCAGCATGACTCCTCCTCAAATCCCCAGACCCATTGATCTGAGC
TTCTTAACGAACAAAGTCACTCCTCCCAGTGCTCGTGGTGAGAAGAAAATAGTAGATCCTGATGCTAATTCTTTAGATTTTGATTTTTTCTAGTCTGGATAATGCCCTGCAATCCAATCTTCAGAAACTTGCTTATATTTGTTGCTCAATTTTCAAGGAATTTATAAGTTTGTATTGTAGGCTGTGCCTATGTTTCAGTA
AAATCTTCAGTCCAACCTGGATTTTTTTGCAGAAGTGCCTCAAAAATCACAGTGGATTACGAGCTTCTTTTTAAGCTGTGTATCATACTTTTCCCTTACAAATTGGTTGCGATTAAACTATAAACTTCAGTTATCTGCAAATGTAGTTTCACTTATTTTTTAGCTTACATATAAATAAATGGAAGCCTACTAAAGTTTCA
ATAATGGAGAGGATCTCAGAGCTTATTATAAAGCTAATTATTGTCAATGGCTGTGAAAAAGGAGCAATAATTGTCAAATGTGTCCCTGCTATTCTGTAACTTAGGTCACTTTTGGTGTTTATAATTGACGAATGTGGCCCTGCAATTCTGTAACATAGGTCACTTTTTGTGTTTTGTATGTTGTGGCCCCATGCAATTCT
TGTAACTTGTGGTAAACTTTTGATGCTGGTTCGGAAAAGTAAGTACTTTATGATGACTTCCTGCTTTTCGGCATAGGCTGTTTTAAATTTCTTTATAATGATGACATGCTGAGGTTGTTCTTGGTTTGATTATTGACCATTCTAATTTAGAATCGAC