sp|Q9FNX5|DRP1E_ARATH ----> Phragmoplastin DRP1E

Intron 1 GTAATGTGTA ... CTTCCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.77	6.8	4	5
A2	0	5.2	0	0
A3	40	3	2	2
A4	16.57	10.4	2	1
A5	46	2.4	2	1.5
A6	30	7	3	3
A7	31.79	17.2	8	2
A8	36.92	19	11	2
A9	27.71	18	6.5	0
A10	24.79	12.2	4	0

>TRINITY_DN43532_c0_g1_i7 from sample A9 / Example spliced isoform

ATTCCCCGCAGGTTTGAGAATTGAATTGCAGGCGAGCGAAATCTTCTATTTTAATGGCGTTTATCCTAGTGGGAACCAGAGGCTAGGGCGGTACATGCAGAACCGTCATCCTTGGAGGAGTACACACAGCTCTTAAGCCTAGGAGGTTAAATCCAGGGTTTACAGAATGGCTTATACATTGCAGGCGCCTGTGCTGTTAG
ATAAAAGGTGCATTCTAGGGCAAGCGGTTCGGCTTGTTCACCCTGGTCTGGCCGTGAAATGGAACCCTAACACGGTCGGGAGGCCTGGCATTGGGCGCCGTAATGGAACAGTTACCTGCAAGTCCTGGTTGAATACAGAGCCTTTTCAGTGCGATGCTCATGGTGGATGGTCACACGGTGAAGGCAGTCTCAAGAGGGAG
AAGAAAAAAGGTATACCACCCCTCATTTTGGTTGGAGGTGGAATGTCACTTGCAATTTGCCTTGCCGTTTTGGTACACACGGTCTCAAACAAAGGTTTCATGGCCCGTTTATGTCCACCAATGGTAGAGATTCATCAATTATTTACACCATTGACTTCTCGAGCACTTGCCCCCATGAAGACTCTTGTTACTGCACTTTC
CCCGGCAAAACCTTTTGATCACAGTTTGTCTGATGCATCAAAGACAATGCCTACCAACTTGGAGGACAAAGATAGGAAAGAAAAAGATTCAGATACCATCAAAATTGAGGTCAGCCGTGCGCCAGCAGAAACAAAGCTTGGGAAAATGTTTATCCCTCCTGCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGG
AGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATT
CAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGGTATGACTACAATG
ATAATGAGCCAAAAAAGTGGTTCTAGCATGCACTTGTATGTAAAAATTTTGCACAGATAATAACAAATGTTAATATCAACAATTACTTCACAAATATTCATGCATTCCAGATAGCACTTTAAAAGAGTGACCTGTTTGCAGTCCAGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCA
TGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGTGAGTCAACTTCTACTTGTAAAGTCATAGGGATCTTAGCTTAAATAGTTAAATGGAGCGACAGTTGCTGTTAGATAGCGTGCTAATTTCAACTTTAGTATCCAGG

>TRINITY_DN43532_c0_g1_i4 from sample A9 / Example unspliced isoform:

CTGTATAATCTTCTAAATATCACATGTGCAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCC
AAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGgtaatgtgtaatttttacaagctttttcgtctatttttgtgagctcgtcactcaaagaaatgcaatatctggattcatttctcgatactggtattttttcttccttcctacagCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAA
CACAATCACTTACCACAAATCGATAAAAGG

Intron 2 GTATGACTAC ... TGCAGTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.52	5	1.8	4
A2	10.09	6	0	1.4
A3	38.38	3.9	3.2	1.7
A4	12.12	18	2.3	2.7
A5	52.35	1	2.2	0
A6	26.96	10	5	2.4
A7	17.65	27	4.8	6.8
A8	29.63	19	10.1	6
A9	23.97	22.9	9.1	5.4
A10	25.71	23.9	4.8	11.8

>TRINITY_DN120950_c6_g2_i7 from sample A1 / Example spliced isoform

TCTATGGTCAAATATTATTGTGAAGGTGTATGGTTTATTTACTTGTTATGTTTCTTCTTCTATAGCAGAAACAAAGCTTGGGAAAATGTTTATCCCTCCTGCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAAT
ATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGC
ATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGA
TGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAG
AGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGT
TTGAAGCTCGAGGTTGATGAACTAAAGAAGACAATATTAAAAGAGGAAACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAACGCG
AACTCAAGCTCGGGGTGAAATGTTGGCAGAAGCTGTGAAAAGATGGAAGTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATGACTTATGCTACCTTGCAAC
AACATGCTGTAAAGTCATTGAAAGGACTGGAGGATAGAGTTCAGAATCTCCAAATTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCT
GTTCTATCTCTCTCTCATTTTTGCATGAGTTTAAGAAATCCATCTTAAATTTTCTCTTTGCATCCCATGATTTTGTAAGTTTGCAATGGGTGTTGATAACTTGGTATTGTATCAAACATCATGAATAAAATTAAATTTAGTTGTGTGAAGGAGA

>TRINITY_DN120950_c6_g2_i5 from sample A1 / Example unspliced isoform:

TCGACAGATTTTGCGTCTTTCTTCTCTCCAAGCAACTTATGTTTGGCAGGAGCTTCTACGTTATTTTAATTATTTGAAGTTATCTTTAGTTCTTACAATCTAAACCACATGTCAACATTTATTGAATGCTTTTAACCTTGTGTCCTGGCTATGTATCATAGGAGCTGCAATTTTGATATGTTTTTTGTCATATCTTAAAG
AAATTAAGTGTGATGCCAAATATTCAATTTTTTTTTCGTTGAATGTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTT
GATGATATAACACCACAAAATCCAGATTTCCCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGGTAATGTGTAATTTTTACAAGCTTTTTCGTCTATTTTTGTGAGCTCGTCAC
TCAAAGAAATGCAATATCTGGATTCATTTCTCAGATACTGGTATTTTTTCTTCCTTCCTACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGgtatgactacaatgataatgagccaaaaaagtggttctagcatgcacttgtatgtaaaaat
tttgcacagataataacaaatgttaatatcaacaattacttcacaaatattcatgcattccagatagcactttaaaagagtgacctgtttgcagtccagACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTAC
ACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAAT
CTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGTTTGAAGCTCGAGGTTGATGAACTAAAG
AAGACAATATTAAAAGAGGAAACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAACGCGAACTCAAGCTCGGGGTGAAATGTTGGC
AGAAGCTGTGAAAAGATGGAAGTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATGACTTATGCTACCTTGCAACAACATGCTGTAAAGTCATTGAAAGGAC
TGGAGGATAGAGTTCAGAATCTCCAAATTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCTGTTCTATCTCTCTCTCATTTTTGCATG
AGTTTAAGAAATCCATCTTAAATTTTCTCTTTGCATCCCATGATTTTGTAAGTTTGCAATGGGTGTTGATAACTTGGTATTGTATCAAACATCATGAATAAAATTAAATTTAGTTGTGTGAAGGAGA

Intron 3 GTGAGATTTC ... ATAAAACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	13	11	2
A2	33.33	12	6	6
A3	24.32	28	9	9
A4	35.29	11	3	9
A5	0	3	0	0
A6	19.63	43	14	7
A7	21.4	90	25	24
A8	24.21	108	26	43
A9	20.17	95	26	22
A10	25.32	59	17	23

>TRINITY_DN43032_c0_g1_i7 from sample A6 / Example spliced isoform

ATTGAATTGCAGGCGAGCGAAATCTTCTATTTTAATGGCGTTTATCCTAGTGTCAACCAGGGGCTAGGGCGGTACATGCAGAACCGTCATCCTTGGAGGAGTACACACAGCTCTTAAGCCTAGGAGGTTAAATCCAGGGTTTACAGAATGGCTTATACATTGCAGGCGCCTGTGCTGTTAGATAAAAGGTGCATTCTAGG
GCAAGCGGTTCGGCTTGTTCACCCTGGTCTGGCCGTGAAATGGAACCCTAACACGGTCGGGAGGCCTGGCATTGGGCGCCGTAATGGAACAGTTACCTGCAAGTCCTGGTTGAATACAGAGCCTTTTCAGTGCGATGCTCATGGTGGATGGTCACACGGTGAAGGCAGTCTCAAGAGGGAGAAGAAAAAAGGTATACCAC
CCCTCATTTTGGTTGGAGGTGGAATGTCACTTGCAATTTGCCTTGCCGTTTTGGTACACACGGTCTCAAACAAAGGTTTCATGGCCCGTTTATGTCCACCAATGGTAGAGATTCATCAATTATTTACACCATTGACTTCTCGAGCACTTGCCCCCATGAAGACTCTTGTTACTGCACTTTCCCCGGCAAAACCTTTTGAT
CACAGTTTGTCTGATGCATCAAAGACAATGCCTACCAACTTGGAGGACAAAGATAGGAAAGAAAAAGATTCAGATACCATCAAAATTGAGGTCAGCCGTGCGCCAGAAACAAAGCTTGGGAAAATGTTTATCCCTCCTGCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATC
ACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGA
TTATACCTAGTAAGTTATCTAATGATGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGGTATGACTACAATGATAATGAGCCAAAAAAGTGGTT
CTAGCATGCACTTGTATGTAAAAATTTTGCACAGATAATAACAAATGTTAATATCAACAATTACTTCACAAATATTCATGCATTCCAGATAGCACTTTAAAAGAGTGACCTGTTTGCAGTCCAGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATG
CTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAG
ATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGTTT
GAAGCTCGAGGTTGATGAACTAAAGAAGACAATATTAAAAGAGGAAACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAATGCGAA
CTCAAGCTCGGGGTGAAATGTTGGCAGAAGCTGTGAAAAGATGGAAGTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATTACTTATGCTACCTTGCAACAA
CATGCTGTAAAGTCATTGAAAGGACTGGAGGATAGAGTTCAGAATCTCCAAATTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCTGT
TCTATCTCTCTCTCATTTTTTCATGAGTTTAAGAAATCCATCTTAAATTTTCTCTTTGCATCCCATGATTTTGTAAGTTTGCAATGGGTGTTGATAACTTGGTATTGTATCAAACATCATGAATAAAATTAAATTTAGTTGTGTGAAGGAGATAATGTTCTTTCTAAACATCCCTAGTCAAAGCAATGTGGCATTGCATG
TTCTGTGATACACTGATATGTGATGCTGTACTATGTTTGATTTCATATCAGTCCTTACCTAGTTTTATTTTGTTGAACTTGCCAGAATTTAGTGGCCCTCGATGCAAAAAAATCTAACCAAGG

>TRINITY_DN43032_c0_g1_i3 from sample A6 / Example unspliced isoform:

ATTGAATTGCAGGCGAGCGAAATCTTCTATTTTAATGGCGTTTATCCTAGTGTCAACCAGGGGCTAGGGCGGTACATGCAGAACCGTCATCCTTGGAGGAGTACACACAGCTCTTAAGCCTAGGAGGTTAAATCCAGGGTTTACAGAATGGCTTATACATTGCAGGCGCCTGTGCTGTTAGATAAAAGGTGCATTCTAGG
GCAAGCGGTTCGGCTTGTTCACCCTGGTCTGGCCGTGAAATGGAACCCTAACACGGTCGGGAGGCCTGGCATTGGGCGCCGTAATGGAACAGTTACCTGCAAGTCCTGGTTGAATACAGAGCCTTTTCAGTGCGATGCTCATGGTGGATGGTCACACGGTGAAGGCAGTCTCAAGAGGGAGAAGAAAAAAGGTATACCAC
CCCTCATTTTGGTTGGAGGTGGAATGTCACTTGCAATTTGCCTTGCCGTTTTGGTACACACGGTCTCAAACAAAGGTTTCATGGCCCGTTTATGTCCACCAATGGTAGAGATTCATCAATTATTTACACCATTGACTTCTCGAGCACTTGCCCCCATGAAGACTCTTGTTACTGCACTTTCCCCGGCAAAACCTTTTGAT
CACAGTTTGTCTGATGCATCAAAGACAATGCCTACCAACTTGGAGGACAAAGATAGGAAAGAAAAAGATTCAGATACCATCAAAATTGAGGTCAGCCGTGCGCCAGAAACAAAGCTTGGGAAAATGTTTATCCCTCCTGCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATC
ACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGA
TTATACCTAGTAAGTTATCTAATGATGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAG
CGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAA
TTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAA
AGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGTTTGAAGCTCGAGGTTGATGAACTAAAGAAGACAATATTAAAAGAGGAAACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTA
ACAATTTCCAGgtgagatttctatcattgttcttgcatcaaatgttcagatatagagagtagtataaaatttccttttttaacagcattggatgtcagtatgtcttctgaagatcaaacttgaattccttgtgccaatataaaaccagGCTGTGGGTAGAGGAAGAGGCAATGCGAACTCAAGCTCGGGGTGAAATGTTG
GCAGAAGCTGTGAAAAGATGGAAGTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATTACTTATGCTACCTTGCAACAACATGCTGTAAAGTCATTGAAAGG
ACTGGAGGATAGAGTTCAGAATCTCCAAATTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCTGTTCTATCTCTCTCTCATTTTTTCA
TGAGTTTAAGAAATCCATCTTAAATTTTCTCTTTGCATCCCATGATTTTGTAAGTTTGCAATGGGTGTTGATAACTTGGTATTGTATCAAACATCATGAATAAAATTAAATTTAGTTGTGTGAAGGAGATAATGTTCTTTCTAAACATCCCTAGTCAAAGCAATGTGGCATTGCATGTTCTGTGATACACTGATATGTGA
TGCTGTACTATGTTTGATTTCATATCAGTCCTTACCTAGTTTTATTTTGTTGAACTTGCCAGAATTTAGTGGCCCTCGATGCAAAAAAATCTAACCAAGG

Intron 4 GTAAAAGTTA ... ACATGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.03	7	6	5.5
A2	31.59	7	5	1.5
A3	29.73	13	7	4
A4	29.57	13	4	7
A5	30	2	1	0.8
A6	3.94	9	0	0.8
A7	17.55	24	8	2.3
A8	6.8	22	0	3.3
A9	16.7	23	7	2.3
A10	20.98	24	11	1.8

>TRINITY_DN105108_c0_g1_i9 from sample A4 / Example spliced isoform

GCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTT
TGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACA
ATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCC
ATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTTGAGCA
GAAGTTTTTAACAAGTTTGGAGCCCGAGAAGTGATCCTTCAGGGGATAAGACATTTGGAATTGTCAAATGCTCACAGGCTGATATTAACAAGAGCGTGGACATGATTGCTGCCCGGGGAAGGAAAATGAATATTTTGCAACTAATCCTGATTATAAACACTTATCAAGTCG

>TRINITY_DN105108_c0_g1_i8 from sample A4 / Example unspliced isoform:

GCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGgtaaaagttaagtaaaaatctggttttcatcgaagaactgaatgtgcaaagcttcaaccc
atttttccaacagattttcaagaggtctgtataatcttctaaatatcacatgtgcagAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTTTGAGTTTCTGTTTTCTAACTACTTCGATAAGCCATT
TATGATTTTGATTGTAGGGATCAGCGATAAATTTCTAATAGGTATATAGAAAGTTTAGAAACTACATTGACTTTTATGAAACCTTGATTGAGTCAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGAC
AGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAG
CAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAG
CTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGTTTGAAGCTCGAGGTTGATGAACTAAAGAAGACAATATTAAAAGAGGAA
ACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAATGCGAACTCAAGCTCGGGGTGAAATGTTGGCAGAAGCTGTGAAAAGATGGAA
GTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATTACTTATGCTACCTTGCAACAACATGCTGTAAAGTCATTGAAAGGACTGGAGGATAGAGTTCAGAATC
TCCAATTTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCTGTTCTATCTCTCTCTCATTTTTTCATGAGTTTAAGAAATCCATCTTAA
ATTTTCTCTTTGCATCCCATGATTT

Intron 5 GTAATGTGTA ... CTTCCTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.66	7.1	4	4.4
A2	0	5.4	0	0
A3	40	3	2	1.4
A4	16	10.8	2	1
A5	47	2.3	2	1.5
A6	30	7	3	2.2
A7	30.99	16.5	7.4	1.7
A8	36.29	19.5	11	1.6
A9	27.68	19	6.9	0
A10	24.47	12.4	4	0

>TRINITY_DN120950_c6_g2_i7 from sample A1 / Example spliced isoform

TCTATGGTCAAATATTATTGTGAAGGTGTATGGTTTATTTACTTGTTATGTTTCTTCTTCTATAGCAGAAACAAAGCTTGGGAAAATGTTTATCCCTCCTGCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAAT
ATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGC
ATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGA
TGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAG
AGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGT
TTGAAGCTCGAGGTTGATGAACTAAAGAAGACAATATTAAAAGAGGAAACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAACGCG
AACTCAAGCTCGGGGTGAAATGTTGGCAGAAGCTGTGAAAAGATGGAAGTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATGACTTATGCTACCTTGCAAC
AACATGCTGTAAAGTCATTGAAAGGACTGGAGGATAGAGTTCAGAATCTCCAAATTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCT
GTTCTATCTCTCTCTCATTTTTGCATGAGTTTAAGAAATCCATCTTAAATTTTCTCTTTGCATCCCATGATTTTGTAAGTTTGCAATGGGTGTTGATAACTTGGTATTGTATCAAACATCATGAATAAAATTAAATTTAGTTGTGTGAAGGAGA

>TRINITY_DN120950_c6_g2_i5 from sample A1 / Example unspliced isoform:

TCGACAGATTTTGCGTCTTTCTTCTCTCCAAGCAACTTATGTTTGGCAGGAGCTTCTACGTTATTTTAATTATTTGAAGTTATCTTTAGTTCTTACAATCTAAACCACATGTCAACATTTATTGAATGCTTTTAACCTTGTGTCCTGGCTATGTATCATAGGAGCTGCAATTTTGATATGTTTTTTGTCATATCTTAAAG
AAATTAAGTGTGATGCCAAATATTCAATTTTTTTTTCGTTGAATGTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTT
GATGATATAACACCACAAAATCCAGATTTCCCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGgtaatgtgtaatttttacaagctttttcgtctatttttgtgagctcgtcac
tcaaagaaatgcaatatctggattcatttctcagatactggtattttttcttccttcctacagCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGGTATGACTACAATGATAATGAGCCAAAAAAGTGGTTCTAGCATGCACTTGTATGTAAAAAT
TTTGCACAGATAATAACAAATGTTAATATCAACAATTACTTCACAAATATTCATGCATTCCAGATAGCACTTTAAAAGAGTGACCTGTTTGCAGTCCAGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTAC
ACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAAT
CTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGTTTGAAGCTCGAGGTTGATGAACTAAAG
AAGACAATATTAAAAGAGGAAACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAACGCGAACTCAAGCTCGGGGTGAAATGTTGGC
AGAAGCTGTGAAAAGATGGAAGTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATGACTTATGCTACCTTGCAACAACATGCTGTAAAGTCATTGAAAGGAC
TGGAGGATAGAGTTCAGAATCTCCAAATTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCTGTTCTATCTCTCTCTCATTTTTGCATG
AGTTTAAGAAATCCATCTTAAATTTTCTCTTTGCATCCCATGATTTTGTAAGTTTGCAATGGGTGTTGATAACTTGGTATTGTATCAAACATCATGAATAAAATTAAATTTAGTTGTGTGAAGGAGA

Intron 6 GTAAGGGTCA ... ACATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.22	79	2	11
A2	19.15	38	1	9
A3	14.04	49	0	8
A4	14.75	52	1	9
A5	23.81	32	2	10
A6	25.34	165	0	56
A7	21.02	139	0	37
A8	22.58	144	0	42
A9	21.91	139	0	39
A10	23.58	94	1	29

>TRINITY_DN111589_c1_g1_i3 from sample A5 / Example spliced isoform

GCTTGGGAAAATGTTTATCCCTCCTGCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATTTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACA
TTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTG
ACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGT
TACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAG
CAGAGAAAATTTTGGAATTTGAGCAGAAGTTTTTAACAAGTTTGGAGCCCGAGAAGTGATCCTTCAGGGGATAAGACATTTGGAATTGTCAAATGCTCACAGGCTGATATTAACAAGAGCGTGGACATGATTGCTGCCCGGGGAAGGAAAATGAATATTTTGCAACTAATCCTGATTATAAACACTTATCAAGTCGAATG
GGCTACTTTTTGAGGAACTGAAGAATGCCATTAAGTCACGCATACCGAGTATTCTGTCTTTGATAACCAAAACTATAGATGAACTTGAATCAGAGATGGATCATCTTGGCAGACCTATTGCTGTTGATGCAGGGGCACAACTATATACAATTTTGGAGCTTTGTCGTGCTTTTGATCGTATTTTCAAAGAGCATCTAGAT
GGAGGACGACCTGGAGGGGAGCGTGTCTATGGAGTCTTTGATAACCAGCTCCCAGCAGCTTTGAAAAAGTTACCTTTTGACCGCCACCTTTCTTTGCAGAATGTTAGGAAGGTGGTCTCTGAGGCTGATGGTTATCAACCTCACTTGATAGCTCCTGAGCAAGGATATCGAAGACTTATTGATGGTTCTCTTGGTTATTT
CAAAGGCCCTGCTGAAGCATCTGTTGATGCGGTGCACTTTATCTTGAAGGAACTTGTTCGTAAATCTTTAAATGAAACACAGGAGTTGAAGCGCTTCCCCACCCTTCAAGCAGAAGTAGCTGCTGCTGCAAATGAAGCTTTAGAGAGATTCCGTGAGGATGGCCGTAAAACTGCATTAAGATTAGTGGAAATGGAGTCTA
GTTATTTGACAGTTGATTTTTTCCGTAAGCTTCCTCAAGAAGTGGAGAAAAGTGGAAATCCTACAGCATCTGCCATGGATCGTTATACAGAGGCTCATCTCAGAAGAATAGGTTCCAATGTTTCTGCTTACGTGGGCATGGTGTGTGAGACACTAAAGAATTCTATCCCTAAAGCTGTGGTCTATTGTCAAGTTCGTGAG
GCCAAGAACGCTTTGCTCAACCACTTTTATACTCAAGTGGGCAGAAAGGAAGGTAAGCAGCTTGCAGCTTTGTTAGATGAAGATCCAGCCTTGATGGAACGTAGGGAAGCATGTGCTAAAAGGCTTGAGCTTTATAAATCAGCACGTGATGAAATAGATGCTGTGTCTTGGGCAAGATAAGAGTAACCATCAGTGTAATT
TGAGTTGTCAAACCCATTCCTTTATATAGATACAAAGTGACGTTCAAAGCAAATTCATGGAGGGGGGCTGGAGTTTGCATTTCTCTTGTACTAAGAGCTCCTTGTAGTATATTTTTTGACTTATGGTGGTGGTTGGAGGGTGCTGATGTGACTTCAGTACAGTTTTATGTGTTTAGGTGATGGTAGCAAGACATTTCTGC
TTCATAGTTCTCAAATATTTGGACATGTGTTCTTTAGAAAATATTAGTTTGAGAGCTGGGATTTCGCGATTGGAAATCGATGATTGTATCAAAATGTTATTAGAGGTGTTTTTTACCATGTATTCTCAATCGCAATCTCTTTTGGTAATGCCATCATAATTTATTAAGCTTGAAAACCAAGTTACATATATTGTTTTCTG
TTCCTCTTCTTCACGGCGCTGTATTTTCACTCATAATAGTGTTTGTCGCTTTGCC

>TRINITY_DN111589_c1_g1_i5 from sample A5 / Example unspliced isoform:

CTCTTGTGGGTTTATTACAGGTGCACTTTATCTTGAAGGAACTTGTTCGTAAATCTTTAAATGAAACACAGgtaagggtcaaacttttgtatgtagtatctattcacgaagagtcttggatttcggcaatcatgatgtggatattagaagggcttaactttctttctaacatttgcagGAGTTGAAGCGCTTCCCCACCC
TTCAAGCAGAAGTAGCTGCTGCTGCAAATGAAGCTTTAGAGAGATTCCGTGAGGATGGCCGTAAAACTGCATTAAGATTAGTGGAAATGGAGTCTAGTTATTTGACAGTTGATTTTTTCCGTAAGCTTCCTCAAGAAGTGGAGAAAAGTGGAAATCCTACAGCATCTGCCATGGATCGTTATACAGAGGCTCATCTCAGA
AGAATAGGTTCCAATGTTTCTGCTTACGTGGGCATGGTGTGTGAGACACTAAAGAATTCTATCCCTAAAGCTGTGGTCTATTGTCAAGTTCGTGAGGCCAAGAACGCTTTGCTCAACCACTTTTATACTCAAGTGGGCAGAAAGGAAGGTAAGCAGCTTGCAGCTTTGTTAGATGAAGATCCAGCCTTGATGGAACGTAG
GGAAGCATGTGCTAAAAGGCTTGAGCTTTATAAATCAGCACGTGATGAAATAGATGCTGTGTCTTGGGCAAGATAAGAGTAACCATCAGTGTAATTTGAGTTGTCAAACCCATTCCTTTATATAGATACAAAGTGACGTTCAAAGCAAATTCATGGAGGGGGGCTGGAGTTTGCATTTCTCTTGTACTAAGAGCTCCTTG
TAGTATATTTTTTGACTTATGGTGGTGGTTGGAGGGTGCTGATGTGACTTCAGTACAGTTTTATGTGTTTAGGTGATGGTAGCAAGACATTTCTGCTTCATAGTTCTCAAATATTTGGACATGTGTTCTTTAGAAAATATTAGTTTGAGAGCTGGGATTTCGCGATTGGAAATCGATGATTGTATCAAAATGTTATTAGA
GGTGTTTTTTACCATGTATTCTCAATCGCAATCTCTTTTGGTAATGCCATCATAATTTATTAAGCTTGAAAACCAAGTTACATATATTGTTTTCTGTTCCTCTTCTTCACGGCGCTGTATTTTCACTCATAATAGTGTTTGTCGCTTTGCC

Intron 7 GTGAGTCAAC ... TAGTATCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	10	0.7	4
A2	21.74	18	0.9	5
A3	45.83	13	1.4	11
A4	50	11	3.5	11
A5	50	5	0	5
A6	32.41	16.7	1.1	8
A7	37.5	15	4.5	9
A8	29.03	22	4.3	9
A9	31.49	45.7	3.4	21
A10	16.13	26	3.8	5

>TRINITY_DN105108_c0_g1_i9 from sample A4 / Example spliced isoform

GCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTT
TGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACA
ATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCC
ATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTTGAGCA
GAAGTTTTTAACAAGTTTGGAGCCCGAGAAGTGATCCTTCAGGGGATAAGACATTTGGAATTGTCAAATGCTCACAGGCTGATATTAACAAGAGCGTGGACATGATTGCTGCCCGGGGAAGGAAAATGAATATTTTGCAACTAATCCTGATTATAAACACTTATCAAGTCG

>TRINITY_DN105108_c0_g1_i2 from sample A4 / Example unspliced isoform:

TTACACAAAGAGCATTTGgtgagtcaacttctacttgtaaagtcatagggatcttagcttaaatagttaaatggagcgacagttgctgttagatagcgtgctaatttcaactttagtatccagGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATG
GCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTTGAGCAGAAGTTTTTAACAAGTTT
GGAGCCCGAGAAGTGATCCTTCAGGGGATAAGACATTTGGAATTGTCAAATGCTCACAGGCTGATATTAACAAGAGCGTGGACATGATTGCTGCCCGGGGAAGGAAAATGAATATTTTGCAACTAATCCTGATTATAAACACTTATCAAGTCG

Intron 8 GTTTGAGTTT ... ATTGAGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.43	6	0.8	0.2
A2	37	6	3.6	6
A3	28.49	10.8	4.4	0.4
A4	37.15	12	7.6	3
A5	40.95	4	2.8	0.2
A6	35.75	5.8	3.4	0.2
A7	19.63	23	5.8	0.8
A8	27.85	19.8	8.2	4.4
A9	14.07	18.8	3.2	1
A10	8	9	0.8	0

>TRINITY_DN105108_c0_g1_i9 from sample A4 / Example spliced isoform

GCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTT
TGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGACAGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACA
ATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAGCAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCC
ATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAGCTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTTGAGCA
GAAGTTTTTAACAAGTTTGGAGCCCGAGAAGTGATCCTTCAGGGGATAAGACATTTGGAATTGTCAAATGCTCACAGGCTGATATTAACAAGAGCGTGGACATGATTGCTGCCCGGGGAAGGAAAATGAATATTTTGCAACTAATCCTGATTATAAACACTTATCAAGTCG

>TRINITY_DN105108_c0_g1_i8 from sample A4 / Example unspliced isoform:

GCTATTGACTCTGCTCAGTGTGAAGCATTGTCGGTACTGGAGAAGTTGCAGATTGTAGAATCACATATTCAGCCTGGAGAATTATGTACTAGGGGGGAATATGCCCGATGGTTTCTTTCTTCAAATTCACTGTTGTCAAGGTAAAAGTTAAGTAAAAATCTGGTTTTCATCGAAGAACTGAATGTGCAAAGCTTCAACCC
ATTTTTCCAACAGATTTTCAAGAGGTCTGTATAATCTTCTAAATATCACATGTGCAGAAGGCCAAGCAACAAGGTGTTTCCTTCAGTCTACATTGAAGGTGTTACACAACTTGCCTTTGATGATATAACACCACAAAATCCCGATTTCGCTTGTATTCAAGgtttgagtttctgttttctaactacttcgataagccatt
tatgattttgattgtagggatcagcgataaatttctaataggtatatagaaagtttagaaactacattgacttttatgaaaccttgattgagtcagGTCTGGCAGAAGCAGGGATTATACCTAGTAAGTTATCTAATGACGTAACATGTAGCATCAGTGCCAAAGAGCAGAGTCACAGTTGTAACATGTTTTACCCAGAC
AGCCCTTTATCACGAGTGGACCTGGTGACCTGGAAAATTGTTGTAGAACACAATCACTTACCACAAATCGATAAAAGGACATTAGAGAAGAAATCTGGGTTCCTTGATGTTCAGCGAATTCGCAAAGATGCATGGCCTGCTATTTTTATTGATGCTACGGCAGGAGATCAAAGTATTACACAAAGAGCATTTGGGTATAG
CAGGCGATTCCAGCCAGATAAGCCCGTTACTAAAGGACAGGTGGCAGTGGCCATCATAAGTGGACAAATGGCAGCTACGGTTTCAGAGGAATTGTCTAGACTTGAAGCTGAAAATTCTGCGAAGGAAGCAGTTCTTCAGGAAATTTTATCAGAGATGATATCCAGAGGAGAAATAAAATCTCTTTGGGAGAAAGATCTAG
CTCAGATAAAACTTCGAAGAATAGAAGCAGAGAAAATTTTGGAATTGGCAGTATCTGAGCTGGAAAAAGAGAAGGATGACAAGGAGAGGAGTTTGCCTTCATTCCTGAAGGAAAAGGCAAGGCTGGAATTTGAACAGAAGTTTTTATCAAGTTTGAAGCTCGAGGTTGATGAACTAAAGAAGACAATATTAAAAGAGGAA
ACAGAAATGTTTTTTGAGAAGCAGCAGCTTGAACAACTCCGACTAGAGTGTGAGGTGGAAAGTGAAGCCTTGTCCAAAGCTAAGTCATTGGTAGAGGCAGAGAAAAGGGCCCTAACAATTTCCAGGCTGTGGGTAGAGGAAGAGGCAATGCGAACTCAAGCTCGGGGTGAAATGTTGGCAGAAGCTGTGAAAAGATGGAA
GTTTACTGGATCTTGCAAGAAATGCTCTTGCTGGTGTCACCATCAGGAAAAGATCAACGAAGACACAGTGCAGGAAGTGGAGAAATCTGCCAATGTTAGTTCTAACCAATTTTGGTCTTCAACTGAATACATTACTTATGCTACCTTGCAACAACATGCTGTAAAGTCATTGAAAGGACTGGAGGATAGAGTTCAGAATC
TCCAATTTTTTTTACTTTCAGCTGCAAACAATGTTCAAAGACTGTTTTCCTCAATCTATGCCTTTTTATTAACAAATATCCTTAAGATATTTCAGAAACTTGATACTGATTCCGCAGAAAGTGGTAATTAGTACTTCTATTTTCTAGAGCCTGTTCTATCTCTCTCTCATTTTTTCATGAGTTTAAGAAATCCATCTTAA
ATTTTCTCTTTGCATCCCATGATTT