sp|Q9M4A2|SPO11_ARATH ----> Meiotic recombination protein SPO11-1

Intron 1 GCTTATCCTA ... AAGGAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.37	4	10	9
A2	75	5	18	12
A3	64.91	10	16	21
A4	83.33	3	16	14
A5	66.67	2	1	7
A6	74	13	33	41
A7	81.31	10	48	39
A8	75.31	10	33	28
A9	75.24	13	32	47
A10	83.16	8	46	33

>TRINITY_DN41723_c1_g1_i8 from sample A8 / Example spliced isoform

TTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAGTATGCCAGTTAGCTGACCTTAATTTGATTGAATATTTCACATACTATGCTTATTGGATTTTTCTTTAATCT
TAACATTCCATTCCGGGAAGTGCCCAAGAATTTTCCATAACAACGAAGGGATTTTATTTATAAATAGTAGTGTTAAGGACTGACTTTATTGCACTCCAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCAT
ACCTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCA
TTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTG
ACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATG
ACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAA
CAAATATTAATGATAAACCTTCA

>TRINITY_DN41723_c1_g1_i9 from sample A8 / Example unspliced isoform:

AAATGAGTGACGCCATCAGAATGGAAGGACGCTCTGTAACACCTGAGGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAG
TGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCA
GTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTgcttatcctattcctgtgcatgttgaacaagttcaaaatattaggagtcttgccaaatatatacttgtcgtggagaaggaaacagTAT
TTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGAAGCCC
CAGTCGATCAGAGTCAGAAGGAGATCATAGCCACTCATAGTAGGTAGTCCCAGCCAGGAGTCCTTACCAATCATAGACATAAGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAG
ACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACA
TTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATA
TTAATGATAAACCTTCA

Intron 2 GCTCCTGTAC ... CCAAGTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	83.39	4.3	25.5	17
A2	65.94	13.4	32.7	19
A3	92.18	1.3	18.5	10
A4	82.82	2.6	15.3	10
A5	92	1	17	6
A6	89.66	3	30	22
A7	71.69	15.3	53.3	24
A8	58.62	12	15	19
A9	87.76	3.6	35	17
A10	74.28	15.7	54.7	36

>TRINITY_DN43438_c0_g2_i5 from sample A7 / Example spliced isoform

GTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAGTATGCCAGTTAGCTGACCTTAATTTGATTGAATATTTCACATACTATGCTTATTGGATTTTTCT
TTAATCTTAACATTCCATTCCGGGAAGTGCCCAAGAATTTTCCATAACAACGAAGGGATTTTATTTATAAATAGTAGTGTTAAGGACTGACTTTATTGCACTCCAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGC
CAACCATACCTGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTG
AAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCA
GAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGC
AATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTT
CATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTCA

>TRINITY_DN43438_c0_g2_i8 from sample A7 / Example unspliced isoform:

CTTTTATTTCAATATTTTTGGGTTTAATCTGGAAGAAAACTGTACAAAGTCGAATACATATAGTTTTGTGATATTCGAAATTCGTCAAAAATGAGTGACGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCT
GCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTgctcctgtacacttgagattgcaaagtgcagagagataatcactgtggaaaggaagggaacagctcgtaggcttgttgtgatgctaagattactccaaatcattcagctgttgctacaacaaaataggcatgcttcta
agagggatgtctattatacagatcctgcagtttttcttgatcaggcaacggtagatcgtgcaattaatgatgtctgcatttattgccagtgtagccgtcataatctaaatgtggtttccccaagtaaagGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACT
GCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGA
GTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGA
CAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACAT
TCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATAT
TAATGATAAACCTTCA

Intron 3 GTGAGTATAT ... GATGTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.19	24	19	1
A2	29.41	12	5	1
A3	52	12	13	0
A4	41.67	14	10	2
A5	23.53	13	4	0
A6	27.94	20.7	8	0
A7	22.58	24	7	0
A8	25.44	14.7	5	0
A9	26.67	22	8	0
A10	37.5	30	18	0

>TRINITY_DN114979_c6_g2_i5 from sample A2 / Example spliced isoform

GGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAG
GAAGGGAACAGCTCGTAGGCTTGGTACATTTGCTATCCTATAATCCTATTGCTCAAATTTACCTCTCAGAATGTCACATGTTTCTTAGTTTATAGATGATTCAGGATATTTTCCTGTCCTATGTTTGTTTCTGACTTTTTGAAGTTTTGACAGAAATACTCATTGTTATTACAAATTTGATCTGATGCAAGTTGTGATGC
TAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGG
TTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGG
AAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTT
CAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTG
TCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCG

>TRINITY_DN114979_c6_g2_i14 from sample A2 / Example unspliced isoform:

GGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAG
GAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGgtgagtatattctgctttatatgggcatatgtgttaatcctgagatgttgataatgcctttttattttttctggt
tttctctcaatggagaatgctaaatccgttgatgcacttttgaggaactgaaataagttataacttgtcaagttatgttgaaattttcagtcaattggttcatctctttatcatacaccagagggttcaaagataaacacaataatcattagtgcaaatacatgcttgtatccaaggaagagacgtcaatgtgaaaatgg
aagtcaggattagattaaggcatttggaaatcagaatatatctattttcattgcacccttatttgatgatacttgtacacctgctcattcttaaataggatggacttggtaatgacaaactggtaggtttttcagcagctatatctatgatgcctactaatctctctaatgtgatgtcacagATCAGGCAACGGTAGATC
GTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTG

Intron 4 GTATGAAGAT ... AGCATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.18	9	0	2
A2	0	1	0	0
A3	0	10	0	0
A4	33.33	2	0	1
A5	0	10	0	0
A6	27.27	40	3	15
A7	15.52	49	0	9
A8	29.67	64	0	27
A9	27.45	37	0	14
A10	32.14	19	0	9

>TRINITY_DN39882_c0_g1_i6 from sample A6 / Example spliced isoform

TCTACATTTTAAGCTTTTGTTTAGATCCCAGCAACCTATGGTAGAATCAATTTAGGTTATTGCTGTATTTTGCAGACATGTTGAGGTCAAAATGTTCTAATCCTACTTATAACCACAGATCACAAAAAATATTTTATCTTTTTGCAGTCAAGTGGATAGTAAAATACATTTATATAATGCAGTTCCAATGGATAGCTTGT
TTGATGGCTTGATGCATTATTACATTTCTGTATAGATGGGTACCCTACATTCTCAAGAGAGGGTGCAGAATAATATTTATATGGATTTCTTGATGATATATTGAAGATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTG
TATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGC
TTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTA
TCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTT
TTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAAC

>TRINITY_DN39882_c0_g1_i7 from sample A6 / Example unspliced isoform:

CAGAAGCACGCTCCAGAATGGAGgtatgaagatcagccagttaaagcattttagATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTG
CAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAAC

Intron 5 GCTCCTGTAC ... CCAAGTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.77	5	25	17
A2	66.67	13	33	19
A3	87.5	2	18	10
A4	80.65	3	15	10
A5	92	1	17	6
A6	90	3	32	22
A7	70.64	16	53	24
A8	58.62	12	15	19
A9	86.67	4	35	17
A10	75.21	15	55	36

>TRINITY_DN43438_c0_g2_i5 from sample A7 / Example spliced isoform

GTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAGTATGCCAGTTAGCTGACCTTAATTTGATTGAATATTTCACATACTATGCTTATTGGATTTTTCT
TTAATCTTAACATTCCATTCCGGGAAGTGCCCAAGAATTTTCCATAACAACGAAGGGATTTTATTTATAAATAGTAGTGTTAAGGACTGACTTTATTGCACTCCAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGC
CAACCATACCTGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTG
AAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCA
GAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGC
AATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTT
CATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTCA

>TRINITY_DN43438_c0_g2_i3 from sample A7 / Example unspliced isoform:

CTTTTATTTCAATATTTTTGGGTTTAATCTGGAAGAAAACTGTACAAAGTCGAATACATATAGTTTTGTGATATTCGAAATTCGTCAAAAATGAGTGACGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCT
GCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTgctcctgtacacttgagattgcaaagtgcagagagataatcactgtggaaaggaagggaacagctcgtaggcttgattactccaaatcattcagctgttgctacaacaaaataggcatgcttctaagagggatgtcta
ttatacagatcctgcagtttttcttgatcaggcaacggtagatcgtgcaattaatgatgtctgcatttattgccagtgtagccgtcataatctaaatgtggtttccccaagtaaagGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTC
CTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGT

Intron 6 GTATGAAGAT ... TTTTCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.67	8.8	0	2
A2	66.67	1	0	2
A3	10.23	9.5	0	1
A4	33.33	2	0.7	1
A5	0	9.8	0	0
A6	23.34	39.5	0	12
A7	18.56	48.4	0.4	11
A8	32.82	63.5	2.4	31
A9	30.32	36.8	1	16
A10	32.64	18.6	0	9

>TRINITY_DN43438_c0_g2_i4 from sample A7 / Example spliced isoform

GTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAGTATGCCAGTTAGCTGACCTTAATTTGATTGAATATTTCACATACTATGCTTATTGGATTTTTCT
TTAATCTTAACATTCCATTCCGGGAAGTGCCCAAGAATTTTCCATAACAACGAAGGGATTTTATTTATAAATAGTAGTGTTAAGGACTGACTTTATTGCACTCCAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGC
CAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGG
TAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTT
GCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGA
TATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGC
ACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAG
TATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTCA

>TRINITY_DN43438_c0_g2_i1 from sample A7 / Example unspliced isoform:

CAGAAGCACGCTCCAGAATGGAGgtatgaagatcagccagttaaagcattttagataagggcttttttatataattcttgtgttcttaggattccaactttcataatttacgaagatggatcttgaatcttgaagttaaaatttctcttttcaacagATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAG
ATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATAT
TTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTCA

Intron 7 GTACAATTGG ... TTTGGAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22.22	14	4	4
A2	15.59	16.3	3	3
A3	20.63	25	5	8
A4	20	10	3	2
A5	7.69	6	1	0
A6	12.9	27	3	5
A7	20.59	27	5	9
A8	13.25	36	4	7
A9	22.39	52	12	18
A10	25.49	19	8	5

>TRINITY_DN43093_c4_g2_i2 from sample A10 / Example spliced isoform

GGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAG
GAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGG
TTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTT
TGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGC
GCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTG
AGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCA
TCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAAC

>TRINITY_DN43093_c4_g2_i1 from sample A10 / Example unspliced isoform:

GGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAG
GAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGG
TTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTT
TGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGgtacaattggccattgatttattcattgaatcccttgaattggttagcaacatcaatatattaacgaatgatagaaatatgcatctcacttgcaatgacacaaatatgttgttatttggaatagGTTCTTGCGGCTTC
TCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCA
TTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACAT
ATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTG
AAACAAATATTAATGATAAAC

Intron 8 GCTCCTGTAC ... CCAAGTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.31	4	25	9
A2	70.97	13.5	33	6
A3	95	1	18	5
A4	85.78	2.5	15	2
A5	94.44	1	17	0
A6	90.91	3	30	3
A7	77.95	15	53	15
A8	55.56	12	15	12
A9	90.92	3.5	35	6
A10	77.48	16	55	21

>TRINITY_DN43093_c4_g2_i8 from sample A10 / Example spliced isoform

GGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTG
CACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGT

>TRINITY_DN43093_c4_g2_i4 from sample A10 / Example unspliced isoform:

GGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTgctcctgtacacttgagattgcaaagtgcagagagataatcactgtggaaag
gaagggaacagctcgtaggcttgttgtgatgctaagattactccaaatcattcagctgttgctacaacaaaataggcatgcttctaagagggatgtctattatacagatcctgcagtttttcttggtttccccaagtaaagGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGA
AATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGT

Intron 9 GTAAGACGTG ... TGCACTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	12	10
A2	80.95	2	7	10
A3	73.33	2	6	5
A4	72.73	3	6	10
A5	100	0	8	1
A6	70.37	4	11	8
A7	92	1	8	15
A8	55.56	2	2	3
A9	70	3	2	12
A10	61.9	4	1	12

>TRINITY_DN39509_c0_g3_i5 from sample A9 / Example spliced isoform

AATTAACGTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATAC
CTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTG
CAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATAT
ATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGC
AACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAG
AATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTAT
TTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTC

>TRINITY_DN39509_c0_g3_i11 from sample A9 / Example unspliced isoform:

AATTAACGTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAgtaagacgtgagggacgctaaatgttcaatcacattctactagtagtatgccagttagctgaccttaatttgattgaatatttcacatactatgcttattgga
tttttctttaatcttaacattccattccgggaagtgcccaagaattttccataacaacgaagggattttatttataaatagtagtgttaaggactgactttattgcactccagACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTG
GTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAG
GCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAG
GAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATG
GTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTG
CAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGA
AAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTC

Intron 10 GTGACTGTTG ... GTCTAGATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.5	7	1	1
A2	30	7	5	1
A3	38.46	8	7	3
A4	60	2	6	0
A5	29.03	11	1	8
A6	25	27	11	7
A7	26.32	35	10	15
A8	27.27	52	18	21
A9	20.83	19	6	4
A10	30.43	8	7	0

>TRINITY_DN104805_c0_g1_i1 from sample A3 / Example spliced isoform

GGAATTTAGATATCATATTTGGAAAAAAGGCAAGTAGTCATAGATTGAAAAGAATATAATCAGGACTTGGAAGTATCAGAAGCCCCAGTCGATCAGAGTCAGAAGGAGATCATAGCCACTCATAGTAGGTAGTCCCAGCCAGGAGTCCTTACCAATCATAGACATAAGCAAATGGCTTATGACGCCAAAGCACTGTCGGC
GCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGC
AATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTT
CATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACC

>TRINITY_DN104805_c0_g1_i4 from sample A3 / Example unspliced isoform:

TGGTTTATAGGGATTAAGACAAATGGTGTATGGGATTAAGATAACGGTATATGCGTTTAGAGATGGTTTGTGTATGGTTTTATAGGGATTAAGATAAATGGTGTATGGGATTGAGATAGCGGTATATGCGTTTAGAGATGCAGAAGATGGAAAACTAACTATGAAAATATTCATTCCTTTGTTTCCTATTAAAGCAAATG
GCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGgtgactgttgtttgttacacgatgagtgaattattggcaaaactttcgctgtcttttatcaagcattttaatcaatgcctttaat
taatcctctatattaaattccctgaatgataatattcttccttaatttaccgttacgctgtctagatagGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTT
CTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTT
TTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACC

Intron 11 GTAAGCATTT ... TTGACATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38	12	6.8	8
A2	35.46	15	6.5	10
A3	13.07	19	2.8	3
A4	6.83	10	0.5	1
A5	11.07	9	2.3	0
A6	9.19	29.5	3	3
A7	11.98	21	1.8	4
A8	17.27	34	1.3	13
A9	14.08	45	4	11
A10	11.17	23	2	4

>TRINITY_DN126874_c5_g1_i5 from sample A1 / Example spliced isoform

CGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGA
TAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGT
CATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATT
GTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACG
CCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGC
ATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTT
GCAGTCGAATATTTCATCTTCATGCG

>TRINITY_DN126874_c5_g1_i3 from sample A1 / Example unspliced isoform:

CGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGA
TAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGT
CATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATT
GTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGgtaagcatttctatttgtaatgtagatgctgtaaatattccatactagatagacatgaaaagctgatcttgcttgttgacattagGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCT
ACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAA
AGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTG
CAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCG

Intron 12 GTAAGTTCAA ... TTGTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.9	8.3	8	6
A2	20.17	12.3	2	0
A3	50.69	11	6	9
A4	46.67	10.7	7	1
A5	0	0.7	0	0
A6	35.29	22	2	0
A7	16.31	32.7	3	0
A8	10.29	23	0	2
A9	30.71	21.7	6	0
A10	2.94	33	1	0

>TRINITY_DN126874_c5_g1_i3 from sample A1 / Example spliced isoform

CGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGA
TAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGT
CATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATT
GTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGTAAGCATTTCTATTTGTAATGTAGATGCTGTAAATATTCCATACTAGATAGACATGAAAAGCTGATCTTGCTTGTTGACATTAGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCT
ACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAA
AGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTG
CAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCG

>TRINITY_DN126874_c5_g1_i4 from sample A1 / Example unspliced isoform:

CGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGA
TAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGT
CATAATCTAAATGTGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTgtaagttcaaattaggtgaataaatatattcaaatatttgtttgtacatttactatttttgtcgtttgatgagtgacaaaaggtattgtttacagGCTTATCCTATTCCTGTGCA
TGTTGAACAAGTTCAAAGTATCCTTGAATGAATTATGTAGTTTTAGTGAGTATTTGAATTGTTGTCTCTTGGATACTTACAAAATGTTCGTGAAGAAGATCAAAGATTAGAAGCTTTGTAGAGAGTACATCACTTACATCATGAACATACTCTAGCGAAGTCCCTGAAAGAGAGGTCATTTTCATTATTCCCTTGCTTTG
TTAATTATTTAATATTGTAGATGAAAAGAACCCAAAGTCTATCTGATAATACATCCACATAAAATATCGGTTGATGCACTTGTATTCCTATAAAAACAGTGGGAAAACAGGTTTATTGGATCAAATAAGATATTATTTCATACAATTAGTCTTTTGATTAAAAATTGGAGTACCACAGAGACTTTACGGTGACAAAGAAT
GTCTCTGTTCTTTATTAAATACTGTAGATGAAAATAACCTATTGTCTATTCAATAAGACATTAGGAAAAAGGTTTGTGTACTTGTATTCATCATGAATGAGTGGGAAAAGGGTTACTGGTGGAGTGGCAAAAGGCATATTTAAATCAAATATGATATTATTCCATTCAAATACCATTTTGATCCAGAATTGAATTTTACC
TTAGAGACATTAGTGTGACCAAGATCATCTGCATAAGCATTTGACACATCAACTCTGTTCTGAAAAGGTTAGTAGTCTAATTTCACTTTTAATACACGAGTAATACTTTTAAAAGGTGAATATTTTTGAATCATTGTTCCCATAGTTTGTGCTAGACCATCCATTACTGCTAAGTGCTAACTTGTTATTTTTGAATTACA
AGCTTTTGACAGAGTCCTAGGACTAACAAAACTTCTAAAAGAGACATGTCCTGACATACACTAGTATGATTGAACCTGCCTATGCTGACTCACTGGATGCAAC

Intron 13 GTAAAATCTT ... CTTATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25	9	1	3
A2	33.33	12	2	6
A3	32.26	21	0	10
A4	22.22	14	5	4
A5	22.22	7	0	2
A6	21.15	41	0	11
A7	15.22	39	0	7
A8	22.22	28	1	8
A9	21.67	47	0	13
A10	15.38	33	0	6

>TRINITY_DN97821_c0_g2_i1 from sample A4 / Example spliced isoform

AACACCTGACGCTTTGCTCCAACGTATCAAAGGTATACAAACTGATACTTATTTCCAAAGAATCTTAACGGAATTAAGTATAAAAAATTAACGTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGC
TAAATGTTCAATCACATTCTACTAGTAGTATGCCAGTTAGCTGACCTTAATTTGATTGAATATTTCACATACTATGCTTATTGGATTTTTCTTTAATCTTAACATTCCATTCCGGGAAGTGCCCAAGAATTTTCCATAACAACGAAGGGATTTTATTTATAAATAGTAGTGTTAAGGACTGACTTTATTGCACTCCAGAC
TTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCA
AATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAG
AGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCA
GATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGAC
CTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAG

>TRINITY_DN97821_c0_g2_i2 from sample A4 / Example unspliced isoform:

TAATATTTATATGGATTTCTTGATGATATATTGAAGATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGgtaaaatcttttttggtcattgttggtcttttgtgactttgaaagctctatttatcctaaaacgttcatgtcgtgtcttattgtagTATTTCAAAGATTGTCAAATGATGGATTTT
GTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCG
CCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAG

Intron 14 GTATGCCAGT ... TGCACTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	13	10
A2	54.29	8	9	10
A3	50	6	7	5
A4	61.54	5	6	10
A5	55.56	4	9	1
A6	64.71	6	14	8
A7	84.62	2	7	15
A8	45.45	3	2	3
A9	64.1	7	13	12
A10	63.64	4	2	12

>TRINITY_DN39509_c0_g3_i6 from sample A9 / Example spliced isoform

AATTAACGTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTAT
AAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATT
ATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGT
TCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATT
CTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAA
AGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCA
CTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTC

>TRINITY_DN39509_c0_g3_i11 from sample A9 / Example unspliced isoform:

AATTAACGTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACCATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAgtatgccagttagctgaccttaatttgattgaatatttcacatactatgcttattgga
tttttctttaatcttaacattccattccgggaagtgcccaagaattttccataacaacgaagggattttatttataaatagtagtgttaaggactgactttattgcactccagACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTG
GTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTCGTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAG
GCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAAGGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAG
GAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCATTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATG
GTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTG
CAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGA
AAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACCTTCGGTTGAAACAAATATTAATGATAAACCTTC

Intron 15 GTGACTGTTG ... CGCTGTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.5	7	1	0.6
A2	41.67	7	5	0.6
A3	46.67	8	7	3
A4	75	2	6	0.9
A5	20	4	1	1
A6	29.38	26.4	11	3.9
A7	22.22	35	10	8.8
A8	26.87	49	18	11.2
A9	24	19	6	2.2
A10	46.67	8	7	0

>TRINITY_DN104805_c0_g1_i2 from sample A3 / Example spliced isoform

GTGACGCCATCAGAATGGAAGGACGCTCTGTAACACCTGACGCTTTGCTCCAACGTATCAAAGGTATACAAACTGATACTTATTTCCAAAGAATCTTAACGGAATTAAGTATAAAAAATTAACGTAGAACTTTGCAGTGTATTTAGCTTTTCAAGAATCGATTCCCACGGACTGTAATTTATAGCTGCAGTCCTTTCACC
ATGTTCTAACTCAGTAAGACGTGAGGGACGCTAAATGTTCAATCACATTCTACTAGTAGTATGCCAGTTAGCTGACCTTAATTTGATTGAATATTTCACATACTATGCTTATTGGATTTTTCTTTAATCTTAACATTCCATTCCGGGAAGTGCCCAAGAATTTTCCATAACAACGAAGGGATTTTATTTATAAATAGTAG
TGTTAAGGACTGACTTTATTGCACTCCAGACTTTACACGAAATCTGTTGAAGGACATAGCCGGAGGAAGTCTGCCGTGTGTTGTTATAAATAAGTACCAGACCTACTGTGCGCTTGGTGATGCCAACCATACCTGCTCCTGTACACTTGAGATTGCAAAGTGCAGAGAGATAATCACTGTGGAAAGGAAGGGAACAGCTC
GTAGGCTTGTTGTGATGCTAAGATTACTCCAAATCATTCAGCTGTTGCTACAACAAAATAGGCATGCTTCTAAGAGGGATGTCTATTATACAGATCCTGCAGTTTTTCTTGATCAGGCAACGGTAGATCGTGCAATTAATGATGTCTGCATTTATTGCCAGTGTAGCCGTCATAATCTAAATGTGGTTTCCCCAAGTAAA
GGATTGGTCATGGGATGGTTGCGATTCATAGAGTCGGGAAGAAATGTTGATTGCACACGAAATATTGTCACTGCTTATCCTATTCCTGTGCATGTTGAACAAGTTCAAAATATTAGGAGTCTTGCCAAATATATACTTGTCGTGGAGAAGGAAACAGTATTTCAAAGATTGTCAAATGATGGATTTTGTGAAAAGAACCA
TTGTATCGTTCTCTCGGGAAGGGGATACCCAGATGTCTCAACAAGGAGGTTCTTGCGGCTTCTCATTGATGAGTTGAACATTCCTACCTATGCACTGGTAGATTCTGATCCGTATGGTTTTGATATTTTGGCAACATACCGTTTTGGCTCAATGCAAATGGCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCT
GGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCC
CTATCATTTTCTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTT
TTTTTTTCTTTTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACC

>TRINITY_DN104805_c0_g1_i4 from sample A3 / Example unspliced isoform:

TGGTTTATAGGGATTAAGACAAATGGTGTATGGGATTAAGATAACGGTATATGCGTTTAGAGATGGTTTGTGTATGGTTTTATAGGGATTAAGATAAATGGTGTATGGGATTGAGATAGCGGTATATGCGTTTAGAGATGCAGAAGATGGAAAACTAACTATGAAAATATTCATTCCTTTGTTTCCTATTAAAGCAAATG
GCTTATGACGCCAAAGCACTGTCGGCGCCAGAGATTCGCTGGCTTGGAGCTTTCCCTTCAGACTGTGAGACCTTCAAAATTCCTGATAGATGTCTTATTCCATTGACACAGACAGgtgactgttgtttgttacacgatgagtgaattattggcaaaactttcgctgtcttttatcaagcattttaatcaatgcctttaat
taatcctctatattaaattccctgaatgataatattcttccttaatttaccgttacgctgtctagATAGGAGGAAAGCCGAAGCCATGTTGGAAAGATGCTATCTGCAGAAGCACGCTCCAGAATGGAGATATCAGCTGCACATGATGTGGATCAAAGGCATCAAATTTGAGATTGAGGCAATATCAGCCCTATCATTTT
CTCTTCTGTCACAAGAATATATTCCATCCAAAATAAGAGATGGCTTGTACATATGACTACATTCACCACATTTTGCAACCGACTATGGATAGCACTCATTGAATGAAAATAAGTATTAGTATTTTAAGAAGGCCAGGAATGCATGGTTGACTCCAGATTTGCAGTCGAATATTTCATCTTCATGCGAGTTTTTTTTTCTT
TTATTTAGAAAAAAATCGCAACTTGTATATATTAATGATAAACC