sp|Q9SKX5|PAO2_ARATH ----> Polyamine oxidase 2

Intron 1 GTCAGGGATT ... GGAGGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.62	77	98	73
A2	45.79	58	34	64
A3	41.67	91	71	59
A4	45.45	66	58	52
A5	32.8	42	26	15
A6	25.63	576	207	190
A7	31.56	757	347	351
A8	28.93	829	308	367
A9	29.43	867	432	291
A10	28.61	750	338	263

>TRINITY_DN125075_c1_g2_i6 from sample A2 / Example spliced isoform

AGAAATTCAGAGCTGCTGCAGGACACCATCGACAGAGAATGCGTCAACAACGAAATCAAGATCCCTCCTCCAAATAATTCAAATTCCCTCGGTCAAATCGACTTTGGGCAGGATTCCTTTTTGTCTCCAGGATATTATTGTTCGGAAGGAGCGGTGTCCAGAAGGAAGCTGACTGTATAATGTTCTTGGTTAGAGCGGCC
TTTCATAGAAGCCGCGATTCCAGCCTCAGTAATCCCTTTGTCTGGTGAAATTAGGGCTTCTCCAAAATATTTGAGAATGGTATTTCATAACAAATACCATTCACAAGTACAGGCATAGGCACAGGGATCTTCATGCGCAGCTTGATATGCTGTGGATAATAAGAATAAAGTGCCTC

>TRINITY_DN125075_c1_g2_i11 from sample A2 / Example unspliced isoform:

AGAAATTCAGAGCTGCTGCAGGACACCATCGACAGAGAATGCGTCAACAACGAAATCAAGATCCCTCCTCCAAATAATTCAAATTCCCTCGGTCAAATCGACTTTGGGCAGGATTCCTTTTTGTCTCCAGgtcagggattaatgcaaggaattgtgggtactccgtattacgtagcactggaggtgttgggagggaagga
ttacagtggatgtgtgaagcgcagaggtgatcttgtacacaatgcgagtaatgatcaccctttcttgggagagatgccacaggagatatttgaggcagtagtgagaccttatcgaattctgaaaacattcgctgtgtttattttggtttgaattctgaaaacattggcgtgtttgaattctgattatcgaattagactct
acctggtcagcacgctgattgccggatttttaagctcgtcgttgtaggcaatggaggaacagGATATTATTGTTCGGAAGGAGCGGTGTCCAGAAGGAAGCTGACTGTATAATGTTCTTGGTTAGAGCGGCCTTTCATAGAAGCCGCGATTCCAGCCTCAGTAATCCCTTTGTCTGGTGAAATTAGGGCTTCTCCAAAAT
ATTTGAGAATGGTATTTCATAACAAATACCATTCACAAGTACAGGCATAGGCACAGGGATCTTCATGCGCAGCTTGATATGCTGTGGATAATAAGAATAAAGTGCCTCAACTGAGCATCGTGCATCTGAATTATTTGGGAAAACTGCTGTCTATGTAATTCACTTTTCATCTTTGACTTAAACAACATTCAACTAGCAGT
AACCGTCGTTGATTCGTGCTCTAATCCTTATTGTTCTATCTGTGGTAACAATTAATGTTCTGCTGTCAATTTGTATTTTCCATTTTATGTGAGAATCGATCTATTAAATAAGAGATAAATATTTCGGAGTGAAAGGCTATAACTCTCTTTGTGTTGGGTTAATATAAAAATGAC

Intron 2 GTAATGATCA ... ATCGAATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.8	5	46	66
A2	100	0	34	63
A3	100	0	74	58
A4	100	0	44	67
A5	100	0	16	16
A6	97.52	4	146	168
A7	98.3	5	236	342
A8	99.66	1	221	371
A9	100	0	295	328
A10	100	0	234	297

>TRINITY_DN45239_c0_g3_i4 from sample A6 / Example spliced isoform

AGGTGATCTTGTACACAATGCGAAACTCTACCTGCCGCGATTCCATCGGTCAGCACGCTGATTGCCGGATTTTTAAGCTCGTCGTTGTAGGCAATGGAGGAACAGGATATTATTGTTCGGAAGGAGCGGTGTCCAGAAGGAAGCTGACTGTATAATGTTCTTGGTTAGAGCGGCCTTTCATAGAAGCCGCGATTCCAGCC
TCAGTAATCCCTTTGTCTGGTGAAATTAGGGCTTCTCCAAAATATTTGAGAATGGTATTTCATAACAAAT

>TRINITY_DN45239_c0_g3_i8 from sample A6 / Example unspliced isoform:

CCATCGACAGAGAATGCGTCAACAACGAAATCAAGATCCCTCCTCCAAATAATTCAAATTCCCTCTGTCAAATCGACTTTGGGCAGGATTCCTTTTTGTCTCCAGGTCAGGGATTAATGCAAGGAATTGTGGGTACTCCGTATTACGTAGCACTGGAGGTGTTGGGAGGGAAGGATTACAGTGGATGTGTGAAGCGCAGA
GGTGATCTTGTACACAATGCGAgtaatgatcaccctttcttgggagagatgccacaggagatatttgaggcagtagtgagaccttatcgaattctgaaaacattcgctgtgtttattttggtttgaattctgaaaacattggcgtgtttgaattctgattatcgaattagAACTCTACCTGCCGCGATTCCATCGGTCAG
CACGCTGATTGCCGGATTTTTAAGCTCGTCGTTGTAGGCAATGGAGGAACAGGATATTATTGTTCGGAAGGAGCGGTGTCCAGAAGGAAGCTGACTGTATAATGTTCTTGGTTAGAGCGGCCTTTCATAGAAGCCGCGATTCCAGCCTCAGTAATCCCTTTGTCTGGTGAAATTAGGGCTTCTCCAAAATATTTGAGAAT
GGTATTTCATAACAAAT

Intron 3 GCGTGTTTGA ... ATCGAATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.08	14	75.5	78.5
A2	94.93	4.2	70.5	72.2
A3	88.24	12.2	71.7	75.2
A4	90.12	8.7	74.3	74.2
A5	81.08	5.3	21.8	21.2
A6	81.29	71.3	279.2	290
A7	89.18	51.7	416.7	410.2
A8	85.75	78	434.2	441
A9	89.95	43.8	382.8	379.7
A10	88.22	46.8	341.2	331.3

>TRINITY_DN45239_c0_g3_i9 from sample A6 / Example spliced isoform

CCATCGACAGAGAATGCGTCAACAACGAAATCAAGATCCCTCCTCCAAATAATTCAAATTCCCTCTGTCAAATCGACTTTGGGCAGGATTCCTTTTTGTCTCCAGGTCAGGGATTAATGCAAGGAATTGTGGGTACTCCGTATTACGTAGCACTGGAGGTGTTGGGAGGGAAGGATTACAGTGGATGTGTGAAGCGCAGA
GGTGATCTTGTACACAATGCGAGTAATGATCACCCTTTCTTGGGAGAGATGCCACAGGAGATATTTGAGGCAGTAGTGAGACCTTATCGAATTCTGAAAACATTCGCTGTGTTTATTTTGGTTTGAATTCTGAAAACATTGACTCTACCTCGTCAGCACGCGGATTGCCGGAGTTTTAAGCTCGTCGTTGTAGGCAATGG
AGGAACAGGTGCGATATAAATAGGGTTTATGTTCATCTGGTATGTTTTATCTTTCAATAGCTTAGATTGACTGTTAGAAA

>TRINITY_DN45239_c0_g3_i2 from sample A6 / Example unspliced isoform:

CCATCGACAGAGAATGCGTCAACAACGAAATCAAGATCCCTCCTCCAAATAATTCAAATTCCCTCTGTCAAATCGACTTTGGGCAGGATTCCTTTTTGTCTCCAGGTCAGGGATTAATGCAAGGAATTGTGGGTACTCCGTATTACGTAGCACTGGAGGTGTTGGGAGGGAAGGATTACAGTGGATGTGTGAAGCGCAGA
GGTGATCTTGTACACAATGCGAGTAATGATCACCCTTTCTTGGGAGAGATGCCACAGGAGATATTTGAGGCAGTAGTGAGACCTTATCGAATTCTGAAAACATTCGCTGTGTTTATTTTGGTTTGAATTCTGAAAACATTGgcgtgtttgaattctgattatcgaattagACTCTACCTCGTCAGCACGCGGATTGCCGG
AGTTTTAAGCTCGTCGTTGTAGGCAATGGAGGAACAGGATGTTGCGGAGAGCTCAGTCCGTGTGGCGGGAGAGG