sp|Q9ZSA2|CDPKL_ARATH ----> Calcium-dependent protein kinase 21

Intron 1 GTGAGTTATT ... GATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.05	506	101.5	104
A2	15.46	678	116.5	124
A3	14.23	609	108	101
A4	16.6	442	82	88
A5	16.17	321.5	56.5	62
A6	16.27	6699	1166	1302
A7	15.68	4674	873	869
A8	17.39	5088.5	852	1071
A9	16.43	5160.5	889	1014
A10	13.98	3538	614	575

>TRINITY_DN44996_c1_g1_i4 from sample A6 / Example spliced isoform

CTGTCAAAGAATGTCTACATGATTTCAGAGCTCTGTGATGGAGGAGATCTCCAGCACTTACTCGACCGGTATGGCTATGGCATTCTTTCCGAGGGACAAATCGGCATAATAATCAAATCTCTTGTCAAATCCCTTCAATACTGCCATTCCTGTAAAATTATTCACAGAGATGTAAAACCATCGAGTATTTTCATGAGGTC
AGACAATCCGGACTACTTTTCATGGGTTTTTAGACTTGGAAATTTTGATTTATCAATTTCGAAAGGGGATGAAAATGTAGTCCACGGTAACGTGGGGACATTGGCATATTCTGCTCCAGAGATGCTGCGTGGAAATGGGTACTCGTACGAAGTTGATGTATGGAGCGCGGGAGTGGTATTGTATGTTCTTCTGTGTGGGA
GGACACCTTTTGATGCCGAGAATGAGAGCGCCACGAGTATGTACAAGAAAATACAGAATACAGAACTAGATCTCAGTAGCGGTGCCTGGAGCTCCATATCTGATTCTGCAAAGGAATTGGTTCTTGGAATGTTGCAGAAAGACCGCAAGCAGCGATTTTCTTGCTATGGAATCTTGAGTCATCGTTGGATTTTGGGCAAT
ACAGCGAGGGCCATACGTGGCTTCCAGCTCAACCAGCTTAACCAGCAACACGCATATCGTTTCGATGCAGAGCAAGAAAGATCATAATTTAGGTTGTAAGAGTGTAATAGAGAGTGAGATCGCTTAGATCATAATATGCATATAGCAGATCCTATTGAGATTGAAAAATTATGTTTATAGTAATTTGTAAGTGGAATATC
AGAGAGTGAAGGATTATATAAAGACATATGAGGATATTAATATTGCTGTTGAATGATTTTGAATAACTGATTTCCAGCTCTCAAACCAGAATTCCTAGTTGCAAGAAGCTTCCAAAATGTTTTATATATATGTGTTTGAATTCTTTGTTACAAGAGGG

>TRINITY_DN44996_c1_g1_i2 from sample A6 / Example unspliced isoform:

CAGGGGTGTATGGAGCTCCATATCTGATTCTGCAAAGGAATTGGTTCTTGGAATGTTGCAGAAAGACCGCAAGCAGCGATTTTCTTGCTATGGAATCTTGAgtgagttattattttctttggattctatgtaaaccttgtttgtgaacagtaattaggtcttataagtgcactgcgagatgggtagacgataaattactg
ttttttgaaacatgatgtgggatgttgcagGTCATCGTTGGATTTTGGGCAATACAGCGAGGGCCATACGTGGCTTCCAGCTCAACCAGCTTAACCAGCAACACGCATATCGTTTCGATGCAGAGCAAGAAAGATCATAATTTAGGTTGTAAGAGTGTAATAGAGAGTGAGATCGCTTAGATCATAATATGCATATAGCA
GATCCTATTGAGATTGAAAAATTATGTTTATAGTAATTTGTAAGTGGAATATCAGAGAGTGAAGGATTATATAAAGACATATGAGGATATTAATATTGCTGTTGAATGATTTTGAATAACTGATTTCCAGCTCTCAAACCAGAATTCCTAGTTGCAAGAAGCTTCCAAAATGTTTTATATATATGTGTTTGAATTCTTTG
TTACAAGAGGG

Intron 2 GTATGTTACA ... ATTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.73	402.7	129	69
A2	17.47	451	125	66
A3	19.23	399	112	78
A4	18.28	351.1	94.1	63
A5	21.03	256.7	83.7	53
A6	19.73	2913.4	887.6	545
A7	20.43	2310.6	745.7	441
A8	20.8	2146	637.3	490
A9	19.47	2302.6	728.6	385
A10	20.44	1887.6	613.9	356

>TRINITY_DN115609_c1_g2_i3 from sample A2 / Example spliced isoform

ATGATATTTGATCCCTCTTGGAGCTTGATAGAGCCTAGAATTTAGATTTATGCTGATTAAAAATCCCAAACAAAGGAAATGGAACTCAATCATCATTAAAGAACAAGAAAAGAAAGCACCCTTTTCTCACATCCTTGTCTTGAAGCCGTTCTATGTTCAAACAGAACGAGGCCGGTTGTTCAAATTACAGTTCTATGAGA
AGAGGAGAGCAAGCAGTCAAAATCCGTTCTTCTTCACAGATCATCCGCGCTTCTGTTGATAAGCTTTCAAAGAAGTGCTCTGGTCATCATTTTTTGAGGATCAACGTCGAACCCTCGTATTAAATTTATATTGCCTATCATTTGAAGGTTAAAACCATGATTCGTGCGATGCAACTTTTGTGGCGTCGTCTCATAACATG
TAATCGAGAGGATTCAGAGAGATCTGGCGACCCGAAACTTCCACAAGTGGAAAAGGATTTTGAAATTGCAGAGATTATTGGAAAAGGACGGTTGGGAGTGGTACACAGTTGCGTCCACAGGAAAACGGGTCAGATATTCGCCTGTAAAAAGATGCCCGCTACCACAAATGCATGTCATGAGATGCATATTCTCCAGAACC
ATTTATCTGGTGGTGGAGTACGCTCAGATATCTTGAAGGTCAAGGCCTGTTACGGGGCGGAGTACCTAGGCCGACATAGCTGGACAAAGAATGTCTACATGATTTCAGAGCTCTGTGATGGAGGAGATCTCCAGCACTTACTCGACCGGTATGGCTATGGCATTCTTTCCGAGGGACAAATCGGCATAATAATCAAATCT
CTTGTCAAATCCCTTCAATACTGCCATTCCTGTAAAATTATTCACAGAGATGTAAAACCATCGAGTATTTTCATGAGGTCAGACAATCCGGACTACTTTTCATGGGTTTTTAGACTTGGAAATTTTGATTTATCAATTTCGAAAGGGGATGAAAATGTAGTCCACGGTAACGTGGGGACATTGGCATATTCTGCTCCAGA
GATGCTGCGTGGAAATGGGTACTCGTACGAAGTTGATGTATGGAGCGCGGGAGTGGTATTGTATGTTCTTCTGTGTGGGAGGACACCTTTTGATGCCGAGAATGAGAGCGCCACGAGTATGTACAAGAAAATACAGAATACAGAACTAGATCTCAGTAGCGGTGCCTGGAGCTCCATATCTGATTCTGCAAAGGAATTGG
TTCTTGGAATGTTGCAGAAAGACCGCAAGCAGCGATTTTCTTGCTATGGAATCTTGAGTCATCGTTGGATTTTGGGCAATACAGCGAGGGCCATACGTGGCTTCCAGCTCAACCAGCTTAACCAGCAACACGCATATCGTTTCGATGCAGAGCAAGAAAGATCATAATTTAGGTTGTAAGAGTGTAATAGAGAGTGAGAT
CGCTTAGATCATAATATGCATATAGCAGATCCTATTGAGATTGAAAAATTATGTTTATAGTAATTTGTAAGTGGAATATCAGAGAGTGAAGGATTATATAAAGACATATGAGGATATTAATATTGCTGTTGAATGATTTTGAATAACTGATTTCCAGCTCTCAAACCAGAATTCCTAGTTGCAAGAAGCTTCCAAAATGT
TTTATATATATGTGTTTGAATTCTTTGTTACAAGAGGCTTCCAAAGTGT

>TRINITY_DN115609_c1_g2_i5 from sample A2 / Example unspliced isoform:

ATGATATTTGATCCCTCTTGGAGCTTGATAGAGCCTAGAATTTAGATTTATGCTGATTAAAAATCCCAAACAAAGGAAATGGAACTCAATCATCATTAAAGAACAAGAAAAGAAAGCACCCTTTTCTCACATCCTTGTCTTGAAGCCGTTCTATGTTCAAACAGAACGAGGCCGGTTGTTCAAATTACAGTTCTATGAGA
AGAGGAGAGCAAGCAGTCAAAATCCGTTCTTCTTCACAGATCATCCGCGCTTCTGTTGATAAGCTTTCAAAGAAGTGCTCTGGTCATCATTTTTTGAGGATCAACGTCGAACCCTCGTATTAAATTTATATTGCCTATCATTTGAAGGTTAAAACCATGATTCGTGCGATGCAACTTTTGTGGCGTCGTCTCATAACATG
TAATCGAGAGGATTCAGAGAGATCTGGCGACCCGAAACTTCCACAAGTGGAAAAGGATTTTGAAATTGCAGAGATTATTGGAAAAGGACGGTTGGGAGTGGTACACAGTTGCGTCCACAGGAAAACGGGTCAGATATTCGCCTGTAAAAAGATGCCCGCTACCACAAATGCATGTCATGAGATGCATATTCTCCAGAACC
ATTTATCTGGTGGTGGAGTACGCTCAGATATCTTGAAGGTCAAGGCCTGTTACGGGGCGGAGTACCTAGGCCGACATAGCTGGACAAAGAATGTCTACATGATTTCAGAGCTCTGTGATGGAGGAGATCTCCAGCACTTACTCGACCGGTATGGCTATGGCATTCTTTCCGAGGGACAAATCGGCATAATAATCAAATCT
CTTGTCAAATCCCTTCAATACTGCCATTCCTGTAAAATTATTCACAGAGATGTAAAACCATCGAGTATTTTCATGAGGTCAGACAATCCGGACTACTTTTCATGGGTTTTTAGACTTGGAAATTTTGATTTATCAATTTCGAAAGgtatgttacataaatgttcttggagattaattcgtcaattttgcgtttctttttg
gtatatttgtggagttttcttgggcattctgcatgtcggctgttagtcaattacaatcttcttaataaagcgcactgtcttttcaatgttcattggaaaatttattcatagattcttgataaactattcatagattctttatatttttacagGGGATGAAAATGTAGTCCACGGTAACGTGGGGACATTGGCATATTCTG
CTCCAGAGATGCTGCGTGGAAATGGGTACTCGTACGAAGTTGATGTATGGAGCGCGGGAGTGGTATTGTATGTTCTTCTGTGTGGGAGGACACCTTTTGATGCCGAGAATGAGAGCGCCACGAGTATGTACAAGAAAATACAGAATACAGAACTAGATCTCAGTAGCGGTGCCTGGAGCTCCATATCTGATTCTGCAAAG
GAATTGGTTCTTGGAATGTTGCAGAAAGACCGCAAGCAGCGATTTTCTTGCTATGGAATCTTGAGTCATCGTTGGATTTTGGGCAATACAGCGAGGGCCATACGTGGCTTCCAGCTCAACCAGCTTAACCAGCAACACGCATATCGTTTCGATGCAGAGCAAGAAAGATCATAATTTAGGTTGTAAGAGTGTAATAGAGA
GTGAGATCGCTTAGATCATAATATGCATATAGCAGATCCTATTGAGATTGAAAAATTATGTTTATAGTAATTTGTAAGTGGAATATCAGAGAGTGAAGGATTATATAAAGACATATGAGGATATTAATATTGCTGTTGAATGATTTTGAATAACTGATTTCCAGCTCTCAAACCAGAATTCCTAGTTGCAAGAAGCTTCC
AAAATGTTTTATATATATGTGTTTGAATTCTTTGTTACAAGAGGCTTCCAAAGTGT