NA ----> NA

Intron 1 GTGAGTGTAA ... TTTTCTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	2	4	10
A2	100	0	0	5
A3	56.25	7	2	16
A4	100	0	4	12
A5	88.89	1	5	11
A6	71.43	15	20	55
A7	74.12	11	22	41
A8	55.17	13	8	24
A9	63.64	14	11	38
A10	70	12	14	42

>TRINITY_DN43269_c5_g1_i2 from sample A7 / Example spliced isoform

ATCCCTTCCGGCCAGGGATCGAAACGCGTGGATAGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGGTCTGAGTGCAGCCGCAATATTGATTTCACAGAGACTCCGTTGTTGATCAAGTTATCACTTACTAATTGTGAAGATTGTGCAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTG
CAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATATAAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCA
AGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGGCGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTG
CTGCGTGAGACG

>TRINITY_DN43269_c5_g1_i1 from sample A7 / Example unspliced isoform:

CAGGGATCGAAACGCGTGgtgagtgtaactttaaacgtttgctttctgtttttatttcgttcgcagcaagggattaacggtttctccttctcttggctttttctaaagGATAGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGGTCTGAGTGCAGCCGCAATATTGATTTCACAGAGACTCCGTTGTTGATCAA
GTTATCACTTACTAATTGTGAAGATTGTGCAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATAT
AAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGG
CGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGACG

Intron 2 GTCTGAGTGC ... GATTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	7	5
A2	100	0	4.5	7
A3	100	0	13.5	13.5
A4	100	0	12	6
A5	100	0	9	11
A6	100	0	36.5	54
A7	92.72	3	30.5	47
A8	100	0	26.5	34
A9	100	0	34.5	75
A10	100	0	34	32

>TRINITY_DN43269_c5_g1_i4 from sample A7 / Example spliced isoform

CAGGGATCGAAACGCGTGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATT
TACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGGCGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGAC
G

>TRINITY_DN43269_c5_g1_i1 from sample A7 / Example unspliced isoform:

CAGGGATCGAAACGCGTGGTGAGTGTAACTTTAAACGTTTGCTTTCTGTTTTTATTTCGTTCGCAGCAAGGGATTAACGGTTTCTCCTTCTCTTGGCTTTTTCTAAAGGATAGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGgtctgagtgcagccgcaatattgatttcacagagactccgttgttgatcaa
gttatcacttactaattgtgaagattgtgcagCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATAT
AAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGG
CGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGACG

Intron 3 GTTAGTCAAA ... GCCTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	15	8
A2	93.33	1	15	13
A3	100	0	3	7
A4	100	0	2	4
A5	100	0	15	9
A6	98.52	1	86	47
A7	85.71	4	29	19
A8	96.88	1	35	27
A9	97.35	2	98	49
A10	100	0	40	21

>TRINITY_DN43269_c5_g1_i4 from sample A7 / Example spliced isoform

CAGGGATCGAAACGCGTGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATT
TACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGGCGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGAC
G

>TRINITY_DN43269_c5_g1_i1 from sample A7 / Example unspliced isoform:

CAGGGATCGAAACGCGTGGTGAGTGTAACTTTAAACGTTTGCTTTCTGTTTTTATTTCGTTCGCAGCAAGGGATTAACGGTTTCTCCTTCTCTTGGCTTTTTCTAAAGGATAGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGGTCTGAGTGCAGCCGCAATATTGATTTCACAGAGACTCCGTTGTTGATCAA
GTTATCACTTACTAATTGTGAAGATTGTGCAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAgttagtcaaattattgaaatataagcgtttcttgattgtaaatatggcagtgagaggccacgcgatgttgtgtatat
aagaaatttctactttatggccttgcctttgcagGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGG
CGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGACG

Intron 4 GTGAGTGTAA ... TAAAGGATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	4	10
A2	100	0	0	5
A3	100	0	2	16
A4	100	0	4	12
A5	100	0	5	11
A6	100	0	20	55
A7	91.78	3	22	45
A8	100	0	8	25
A9	100	0	11	40
A10	100	0	14	42

>TRINITY_DN43269_c5_g1_i4 from sample A7 / Example spliced isoform

CAGGGATCGAAACGCGTGCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATT
TACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGGCGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGAC
G

>TRINITY_DN43269_c5_g1_i1 from sample A7 / Example unspliced isoform:

CAGGGATCGAAACGCGTGgtgagtgtaactttaaacgtttgctttctgtttttatttcgttcgcagcaagggattaacggtttctccttctcttggctttttctaaaggatagCTTGCCTAGGGTTTACCTTTTTTTCGTGTAGCCGAGGTCTGAGTGCAGCCGCAATATTGATTTCACAGAGACTCCGTTGTTGATCAA
GTTATCACTTACTAATTGTGAAGATTGTGCAGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATAT
AAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGG
CGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAGACG

Intron 5 GTGAGTGTAA ... TTGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	65	7	4	13
A2	77.78	2	0	7
A3	100	0	2	5
A4	100	0	4	1
A5	100	0	5	10
A6	81.01	15	20	64
A7	90.24	4	22	37
A8	77.78	8	8	28
A9	81.55	19	11	84
A10	86.84	5	14	33

>TRINITY_DN40078_c0_g1_i3 from sample A10 / Example spliced isoform

CAGGGATCGAAACGCGTGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATATAAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAA
TATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGGCGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAG
TATGAGAATCGCTTGCTGCGTGAAACTAGGGTTTGACTCCCAAAAGATCAAGATTGCAGTATGAATTTTGATGTCCTCTGCAGATGTTGCGTAACCAAAGACTGATGTCTGGCTTCAGGGTTTTGATCTGTATCAACGGCATCATCATTCTGCACACAGACTGCTCGAAGGTGATTTGAGGAATGAAGGATAGCAGAAAT
GTTTCGAATCATCGATTTACGAAACATCAAGGAAATATAAAAGTCGGACATTAGAAGCATTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACATTAGAAGCCTTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAA
AATATAATAAGTCGGACATTAGAAGCCTTAAAGATTCTCTCGCCTGTGAA

>TRINITY_DN40078_c0_g1_i2 from sample A10 / Example unspliced isoform:

CAGGGATCGAAACGCGTGgtgagtgtaactttaaacgtttgctttctgtttttatttcgttcgcagcaagggattaacggtttctccttctcttggctttttctaaaggatagcttgcctagggtttacctttttttcgtgtagccgaggtctgagtgcagccgcaatattgatttcacagagactccgttgttgatcaa
gttatcacttactaattgtgaagattgtgcagcggctttatggatcgcagctgaaagctaggttttgatcgttgtgtgcagTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATAT
AAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGG
CGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAAACTAGGGTTTGACTCCCAAAAGATCAAGATTGCAGTATGAATTTTGATGTCCTCTGCAGATGTTGCGTAACCAAAGACTGATGTCTGGCTTCAGGGTTTTGATCTGTATCAAC
GGCATCATCATTCTGCACACAGACTGCTCGAAGGTGATTTGAGGAATGAAGGATAGCAGAAATGTTTCGAATCATCGATTTACGAAACATCAAGGAAATATAAAAGTCGGACATTAGAAGCATTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACATTA
GAAGCCTTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACATTAGAAGCCTTAAAGATTCTCTCGCCTGTGAA

Intron 6 GTGAGTGTAA ... GATTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	2	4	5
A2	88.89	1	0	8
A3	82.35	3	2	14
A4	100	0	4	6
A5	73.33	4	5	11
A6	72.37	21	20	55
A7	83.93	9	22	47
A8	83.97	6.5	8	34
A9	70.42	31.5	11	75
A10	78.05	9	14	32

>TRINITY_DN35657_c0_g1_i2 from sample A9 / Example spliced isoform

GCCAGGGATCGAAACGCGTGCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTATATAAGAAATTTCTA
CTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGACGGCGTGTTTCTTGT
TTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAAACTAGGGTTTGACTCCCAAAAGATCAAGATTGCAGTATGAATTTTGATGTCCACTGCAGATGTTGCGTAACCAAAGACTGATGTCTGGCTTCAGGGTTTTGATCTGTATCAACGGCATCATCATT
CTGCACACAGACTGCTCGAAGGTGATTTGAGGAATGAAGGATAGCAGAAATGTTTCGAATCATCGATTTACGAAACATCAAGGAAATATAAAAGTCGGACATTAGAAGCATTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACATTAGAAGCCTTAAAG
ATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACATTAGAAGCCTTAAAGATTCTCTCGCCTGTGAA

>TRINITY_DN35657_c0_g1_i1 from sample A9 / Example unspliced isoform:

GCCAGGGATCGAAACGCGTGgtgagtgtaactttaaacgtttgctttctgtttttatttcgttcgcagcaagggattaacggtttctccttctcttggctttttctaaaggatagcttgcctagggtttacctttttttcgtgtagccgaggtctgagtgcagccgcaatattgatttcacagagactccgttgttgatc
aagttatcacttactaattgtgaagattgtgcagCGGCTTTATGGATCGCAGCTGAAAGCTAGGTTTTGATCGTTGTGTGCAGTTTAGTAAGGCTTGCTGCCACGGGATTCGTTCTAAAGATCGAGTTAGTCAAATTATTGAAATATAAGCGTTTCTTGATTGTAAATATGGCAGTGAGAGGCCACGCGATGTTGTGTAT
ATAAGAAATTTCTACTTTATGGCCTTGCCTTTGCAGGTGCGCTTGCAGGCGGGTCAACTCATTAATATAATAATTGACCAAGTTTTTCATTTACGGGACGGCCGTGCAGGGCTGTTTTGCCCGGCGGTGCTTCCCGCTACGTTCTTGTAATTGTTTTGGTAAAATCCTTTCTTGCATCTGGATTTGTTCTGCCAGATGAC
GGCGTGTTTCTTGTTTTTTGTGAAGTATAATCCTTTCTTCACATTACATATCAATCAATTGTAAGTATGAGAATCGCTTGCTGCGTGAAACTAGGGTTTGACTCCCAAAAGATCAAGATTGCAGTATGAATTTTGATGTCCACTGCAGATGTTGCGTAACCAAAGACTGATGTCTGGCTTCAGGGTTTTGATCTGTATCA
ACGGCATCATCATTCTGCACACAGACTGCTCGAAGGTGATTTGAGGAATGAAGGATAGCAGAAATGTTTCGAATCATCGATTTACGAAACATCAAGGAAATATAAAAGTCGGACATTAGAAGCATTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACAT
TAGAAGCCTTAAAGATTCTCTCGCCTGTGAAATGAAGGAGTCTAAACATTCGAAGAGTTCAAGAAAATATAATAAGTCGGACATTAGAAGCCTTAAAGATTCTCTCGCCTGTGAA