NA ----> NA

Intron 1 GTTAATAAGC ... AATCTGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50.99	99	94	112
A2	51.77	102	111	108
A3	46.54	116	107	95
A4	50.32	116	113	122
A5	50.93	53	51	59
A6	48.59	73	67	71
A7	51.91	220	240	235
A8	50.6	62	61	66
A9	50.85	159	163	166
A10	50.45	167	168	172

>TRINITY_DN112000_c0_g1_i6 from sample A3 / Example spliced isoform

CTTTTATTTGAACTTCTATCCCATCTCCTCGATCTCGCATGTATTAGCAAACTTGTACCTGCCCTACTCCTGGAAGTGGCGGTTATAAGTCATTGGGTGGTGAAAGACACAGGGAAAGGCAAGCAATCTAACTAAATGTCCAGAATGCAGATGTTACTCTCTAGCAGATACAGAGTTTCAATATAAATAGAAGGTAAGTG
ATCTATCTGGCAGTGGCGATGGCAAAAGCAATGGATGGGGAGTGGATGTTTCATCTGCGACTATTGTCGCAGAGTACAAGGGACTCCACTTATGTCAATGATCCTGCTAACGATGTTACCCTTATTCAATCTGCCGAAAATTCAGAAGATTTGGTGGCCAGAATTTATCCGCAAATCAACAAACTCTTCCAACGTTGCAT
TGCTTCCATGCCCAATTCAAGATCCTCTGCTGGACTTCTCCTTCTGGCAATTCTTCAATTTTTCCTGGACTTTGGGGAGACAACTTTGCATGATGCAGATGCAAGCATCCGAACATTTTTCCGTTCATGTCTAAGCAGACAATTTGTGGATTCTTTAGTAGCTGCAGCAACTCTTAATTTCCTCAACCGGAATAAAACAA
AGTTGTTAAGTTCATTTCCACATCTTCTTCCTGAGTATTTTCCTCTGTTTCTGAAGCTTATTGCATGGAATGGAGGGACGTTAGAAAAAGCCTTTCTGAAGCTTCTTCCTGGAATGATATCTCCTACATCATTTCTGCCCCTGTTTCCCGCTCTCATGGATTTACCAACGCTAGTACTGGCCTTAGAAAGATTAGGGCAG
GGCTCAGGATCGCTAGTCGGAAATAATGCAGCTTCCATTCAAAAAAGTCAAACCCCTGAGAATTTGCTACAACTCATGGATGAAGCTTATACTGGTTCCACCATTGTGGATCGGGGTGGGGATTCTGGAGATGAGAATGTTGGATCTAGGGATGATGTGGAAGCTTTATTTCTTGATCTTCTAAAGGATGAGAATGATGG
ATTAACAGAGCGCCATTGGACATCTCCACGTATGGCTGCTTCACTACAAGCAGCCATGGGTTCAACACAATCAGAAAGGCTAAAACAAGGCTTCAAGATGGCTCCTCGTCTTCTTGAACTATATTTCAGGATTGCATTGCGTGATGTCAATGATTCTCTGATTTGTGCCTTAATACCACTTCTCATGGCAAGAAATAGTA
CTTTGATTCCTGACAAAACGTTTGCTGTTGAG

>TRINITY_DN112000_c0_g1_i3 from sample A3 / Example unspliced isoform:

CTTTTATTTGAACTTCTATCCCATCTCCTCGATCTCGCATGTATTAGCAAACTTGTACCTGCCCTACTCCTGGAAGTGGCGGTTATAAGTCATTGGGTGGTGAAAGACACAGGGAAAGGCAAGCAATCTAACTAAATGTCCAGAATGCAGATGTTACTCTCTAGCAGATACAGAGTTTCAATATAAATAGAAGGTAAGTG
ATCTATCTGGCAGTGGCGATGGCAAAAGCAATGGATGGGGAGTGGATGTTTCATCTGCGACTATTGTCGCAGAGTACAAGGGACTCCACTTATGTCAATGATCCTGCTAACGATGTTACCCTTATTCAATCTgttaataagctctacgaaatctgcaagGCCGAAAATTCAGAAGATTTGGTGGCCAGAATTTATCCGCA
AATCAACAAACTCTTCCAACGTTGCATTGCTTCCATGCCCAATTCAAGATCCTCTGCTGGACTTCTCCTTCTGGCAATTCTTCAATTTTTCCTGGACTTTGGGGAGACAACTTTGCATGATGCAGATGCAAGCATCCGAACATTTTTCCGTTCATGTCTAAGCAGACAATTTGTGGATTCTTTAGTAGCTGCAGCAACTC
TTAATTTCCTCAACCGGAATAAAACAAAGTTGTTAAGTTCATTTCCACATCTTCTTCCTGAGTATTTTCCTCTGTTTCTGAAGCTTATTGCATGGAATGGAGGGACGTTAGAAAAAGCCTTTCTGAAGCTTCTTCCTGGAATGATATCTCCTACATCATTTCTGCCCCTGTTTCCCGCTCTCATGGATTTACCAACGCTA
GTACTGGCCTTAGAAAGATTAGGGCAGGGCTCAGGATCGCTAGTCGGAAATAATGCAGCTTCCATTCAAAAAAGTCAAACCCCTGAGAATTTGCTACAACTCATGGATGAAGCTTATACTGGTTCCACCATTGTGGATCGGGGTGGGGATTCTGGAGATGAGAATGTTGGATCTAGGGATGATGTGGAAGCTTTATTTCT
TGATCTTCTAAAGGATGAGAATGATGGATTAACAGAGCGCCATTGGACATCTCCACGTATGGCTGCTTCACTACAAGCAGCCATGGGTTCAACACAATCAGAAAGGCTAAAACAAGGCTTCAAGATGGCTCCTCGTCTTCTTGAACTATATTTCAGGATTGCATTGCGTGATGTCAATGATTCTCTGATTTGTGCCTTAA
TACCACTTCTCATGGCAAGAAATAGTACTTTGATTCCTGACAAAACGTTTGCTGTTGAG

Intron 2 ATCCATAAAA ... AGAAATTTAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	22	78	28	16
A2	22.61	77	25	20
A3	24.89	86	33	24
A4	23.4	54	20	13
A5	25.71	26	12	6
A6	30.5	98	33	53
A7	22.46	214	63	61
A8	34.55	144	51	101
A9	28.95	162	72	60
A10	26.32	154	74	36

>TRINITY_DN112000_c0_g1_i3 from sample A3 / Example spliced isoform

CTTTTATTTGAACTTCTATCCCATCTCCTCGATCTCGCATGTATTAGCAAACTTGTACCTGCCCTACTCCTGGAAGTGGCGGTTATAAGTCATTGGGTGGTGAAAGACACAGGGAAAGGCAAGCAATCTAACTAAATGTCCAGAATGCAGATGTTACTCTCTAGCAGATACAGAGTTTCAATATAAATAGAAGGTAAGTG
ATCTATCTGGCAGTGGCGATGGCAAAAGCAATGGATGGGGAGTGGATGTTTCATCTGCGACTATTGTCGCAGAGTACAAGGGACTCCACTTATGTCAATGATCCTGCTAACGATGTTACCCTTATTCAATCTGTTAATAAGCTCTACGAAATCTGCAAGGCCGAAAATTCAGAAGATTTGGTGGCCAGAATTTATCCGCA
AATCAACAAACTCTTCCAACGTTGCATTGCTTCCATGCCCAATTCAAGATCCTCTGCTGGACTTCTCCTTCTGGCAATTCTTCAATTTTTCCTGGACTTTGGGGAGACAACTTTGCATGATGCAGATGCAAGCATCCGAACATTTTTCCGTTCATGTCTAAGCAGACAATTTGTGGATTCTTTAGTAGCTGCAGCAACTC
TTAATTTCCTCAACCGGAATAAAACAAAGTTGTTAAGTTCATTTCCACATCTTCTTCCTGAGTATTTTCCTCTGTTTCTGAAGCTTATTGCATGGAATGGAGGGACGTTAGAAAAAGCCTTTCTGAAGCTTCTTCCTGGAATGATATCTCCTACATCATTTCTGCCCCTGTTTCCCGCTCTCATGGATTTACCAACGCTA
GTACTGGCCTTAGAAAGATTAGGGCAGGGCTCAGGATCGCTAGTCGGAAATAATGCAGCTTCCATTCAAAAAAGTCAAACCCCTGAGAATTTGCTACAACTCATGGATGAAGCTTATACTGGTTCCACCATTGTGGATCGGGGTGGGGATTCTGGAGATGAGAATGTTGGATCTAGGGATGATGTGGAAGCTTTATTTCT
TGATCTTCTAAAGGATGAGAATGATGGATTAACAGAGCGCCATTGGACATCTCCACGTATGGCTGCTTCACTACAAGCAGCCATGGGTTCAACACAATCAGAAAGGCTAAAACAAGGCTTCAAGATGGCTCCTCGTCTTCTTGAACTATATTTCAGGATTGCATTGCGTGATGTCAATGATTCTCTGATTTGTGCCTTAA
TACCACTTCTCATGGCAAGAAATAGTACTTTGATTCCTGACAAAACGTTTGCTGTTGAG

>TRINITY_DN112000_c0_g1_i10 from sample A3 / Example unspliced isoform:

TGCTACAACTCATGGATGAAGCTTATACTGGTTCCACCATTGTGGATCGGGGTGGGGATTCTGGAGATGAGAATGTTGGATCTAGGGATGATGTGGAAGCTTTATTTCTTGATCTTCTAAAGGATGAGAATGATGGATTAACAGTTAGAAAAAGCCTTTCTGAAGCTTCTTCCTGGAATGATATCTCCTACATCATTTCT
GCCCCTGTTTCCCGCTCTCATGGATTTACCAACGCTAGTACTGGCCTTAGAAAGATTAGGGCAGGGCTCAGGATCGCTAGTCGGAAATAATGCAGCTTCCATTCAAAAAAGTCAAACCCCTGAGAATTTGCTACAACTCATGGATGAAGCTTATACTGGTTCCACCATTGTGGATCGGGGTGGGGATTCTGGAGATGAGA
ATGTTGGATCTAGGGATGATGTGGAAGCTTTATTTCTTGATCTTCTAAAGGATGAGAATGATGGATTAACAatccataaaagacttttggaattcatgctttctgcatttcagcggtctccgcatttcattgcattactgaagaagccaataatggacaggattggggaagcttatgacagccctgcaaagacagagcta
gcattgcagttatgctgggcaattggtgaacacgggggtggtggcatatctcataaagatgcagctagggaactatttgagagcttggagttgctattatatgaaaatctttcatccagtcgtgttgcattgaatcatgatatggtcagcaattatgaaggagtgattcttaagaagtctgggcaggcaagatttctctg
ttttgtggtgacagctattgcaaagcttgctacataccatcgagagttgatcccaagagctcgtgtatgtttggctaaggttgcacgctctcgatatgtcttggacaaaacagtttggatgcgtgcacgagactatttgggtttaatgaacgagcctgctatatgcctatccattcttgggccttcaagtggttcccacc
attgtcctgcaactgtacgctggagtgaaggtggcacaaaagctgttgcacatatccctttttacctcttgggagaaaaagacggtccaccatttcatgattactctctgtcagacattctggggaagacctgaaagaaagataaccatacctcttgggaatttaattaaactgaagatattctcctcctgtcttgataa
gcgcatcttacattgtactcataatcaatgtaactgaatctctttctgacataccgtatttgtctggcctttacaaactgtctctcatgatggtaatggtaatgttaatttcagatatggtagtctctgtgttgttctggcttaactttgtggcacacattattgtgcattgatttcctaccatatgcaaattgtgggtg
tgcttggaaggtcttcttgccttggaaattttctatctccttgtatcccttcctataattatcagcaaatcttttcaggaaaacaagaatacattggtcattatcatatcatatgaaacaaaattaatgtttacttttgtagagagacttgtattgttttgtctacaataaaagaggacgaggctagtatgatgctgggt
aggaacggtttatgatcagcttattctttgtgagcataaaaattgactttgtatcgaccatcatagaaatttacGAGCGCCATTGGACATCTCCACGTATGGCTGCTTCACTACAAGCAGCCATGGGTTCAACACAATCAGAAAGGCTAAAACAAGGCTTCAAGATGGCTCCTCGTCTTCTTGAACTATATTTCAGGATT
GCATTGCGTGATGTCAATGATTCTCTGATTTGTGCCTTAATACCACTTCTCATGGCAAGAAATAGTACTTTGATTCCTGACAAAACGTTTGCTGTTGAG