NA ----> NA

Intron 1 ATGCTGGGTG ... AATATAAAAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.38	24	43	104
A2	71.65	18	28	63
A3	80.25	16	39	91
A4	82.42	8	22	53
A5	57.14	6	3	13
A6	100	0	2	3
A7	100	0	7	14
A8	42.86	4	1	5
A9	60	3	2	7
A10	75	1	2	4

>TRINITY_DN136483_c3_g1_i4 from sample A1 / Example spliced isoform

TATTTAGGACAGGGTATTACATGATGCTTAGAAGCTATTTAGGACAGGGTATTACATGTTAGCATCCCGTGATATGAACCCCTGAATTACATTAGAGATAAGTTCTATCCAAAAGGTTTTGGTCAGTTCTGGCTGACCACAAGTTGAGAATTCAAGGCTATTGAAATTCCTCTTTGACCAACCTTTTACATCCACATTAT
TGGATGGTTCAACAGTTTCATGACGACTTTGCCAGTATCTATATTTCACAATGTGAGTCTCACGCAATGCAAACGATGATTACGTCCACATCCCAAAATATTTGTACACTAAATCCCGAGCACAAAGTAGGGAATTCTTGATTCAAAAGATGTTGCCAATATTCAGGAGAAGACCAAATATCCAGAATTTTACACATCAA
CAGAGCATAAACATTGACATGAGCCAATCCTGGGTAGGAATGTGACTTCACAGCTTACAAAAACAATCTGCCATGCTTTTCAGATATGTAGTTCTATGTTCTTTAACAATTGGATGAGGATTCTTGGAAATTTGAAATGTAGCATAGCTGTTGCTAAAGTCTGCAGAAAATCCACCTCCATGTCTTTTCAATTACATTGA
GTGCATATATAGCATCCGCTTAACCACAAAACAAAATTAGCCTGACAAGTTGACTACAATCAACTGCCTGTCTGTCTTACACCTATGTCACTCTTACATAATGATGCAAGAAAACATCAAAAGAGGGCTTCTTTAGTTGCAAGAAACCAGCGTCAACCAAATCACTAAAGTTCTGGCATAGTTGTGAAAAAACATACAAG
ATTGAGTGATATTCCACAATGGTTGGGAAGTCTAAAAAGAAACTAGCAAAGGAAAATAAAGGAAAGGGAAATTGGAAAATGGGGTTGTGTATGCCTTCTAGATAACATATTGAGAACATGATTATTGGTTGTTTCCTTCAAGCCCTTGAAAGCATGGCTTCAAGATCGATGCATTCATTTTACATGCCCAATTGTATTTG
GGCTGATTTTCGGAAAGTTTCTAAATCTTTGCATTGAAAGTGAAGATTTACATTTTACATTAGCAATTGCATTGGACCCTCCACGGATGCTCTCGAAAATCTCTCATATGGGTCTTAGCTCTTCCAAACAACATGACTTTACAAATC

>TRINITY_DN136483_c3_g1_i6 from sample A1 / Example unspliced isoform:

TGCTTAGAAGCTATTTAGGACAGGGTATTACatgctgggtggcccaaaaaaaaaacagttatgtaggctggcaatataaaacATGATGCTTAGAAGCTATTTAGGACAGGGTATTACATGTTAGCATCCCGTGATATGAACCCCTGAATTACATTAGAGATAAGTTCTATCCAAAAGGTTTTGGTCAGTTCTGGCTGACC
ACAAGTTGAGAATTCAAGGCTATTGAAATTCCTCTTTGACCAACCTTTTACATCCACATTATTGGATGGTTCAACAGTTTCATGACGACTTTGCCAGTATCTATATTTCACAATGTGAGTCTCACGCAATGCAAACGATGATTACGTCCACATCCCAAAATATTTGTACACTAAATCCCGAGCACAAAGTAGGGAATTCT
TGATTCAAAAGATGTTGCCAATATTCAGGAGAAGACCAAATATCCAGAATTTTACACATCAACAGAGCATAAACATTGACATGAGCCAATCCTGGGTAGGAATGTGACTTCACAGCTTACAAAAACAATCTGCCATGCTTTTCAGATATGTAGTTCTATGTTCTTTAACAATTGGATGAGGATTCTTGGAAATTTGAAAT
GTAGCATAGCTGTTGCTAAAGTCTGCAGAAAATCCACCTCCATGTCTTTTCAATTACATTGAGTGCATATATAGCATCCGCTTAACCACAAAACAAAATTAGCCTGACAAGTTGACTACAATCAACTGCCTGTCTGTCTTACACCTATGTCACTCTTACATAATGATGCAAGAAAACATCAAAAGAGGGCTTCTTTAGTT
GCAAGAAACCAGCGTCAACCAAATCACTAAAGTTCTGGCATAGTTGTGAAAAAACATACAAGATTGAGTGATATTCCACAATGGTTGGGAAGTCTAAAAAGAAACTAGCAAAGGAAAATAAAGGAAAGGGAAATTGGAAAATGGGGTTGTGTATGCCTTCTAGATAACATATTGAGAACATGATTATTGGTTGTTTCCTT
CAAGCCCTTGAAAGCATGGCTTCAAGATCGATGCATTCATTTTACATGCCCAATTGTATTTGGGCTGATTTTCGGAAAGTTTCTAAATCTTTGCATTGAAAGTGAAGATTTACATTTTACATTAGCAATTGCATTGGACCCTCCACGGATGCTCTCGAAAATCTCTCATATGGGTCTTAGCTCTTCCAAACAACATGACT
TTACAAATC

Intron 2 ATGCTGGGTG ... AATATAAAAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.04	41	36	98
A2	61.6	24	21	56
A3	66.27	28	29	81
A4	77.27	10	18	50
A5	48.39	8	2	13
A6	71.43	1	2	3
A7	100	0	7	15
A8	42.86	4	1	5
A9	45.45	3	0	5
A10	75	1	2	4

>TRINITY_DN125687_c3_g1_i6 from sample A2 / Example spliced isoform

TGCTTAGAAGCTATTTAGGACAGGGTATTACATGATGCTTAGAAGCTATTTAGGACAGGGTATTACATGTTAGCATCCCGTGATATGAACCCCTGAATTACCTTAGAGATAAGTTCTATCCAAAAGGTTTTGGTCAGTTCTGGCTGACCACAAGTTAAGAATTCAAGGCTATTGAAATTCCTCTTTGACCAACCTTTTAC
ATCCACATTATTGGATGGTTCAACAGTTTCATGACGACTTTGCCAGTATCTATATTTCACAATGTGAGTCTCACGCAATGCAAACGATGATCACGTCCACATCCCAAAATATTTGTACAGTAAATCCCGAGAACAAAGTAGGGAATTCTTGATTCAAAAGATGTTGCCAATATTCAGGAGAAGACCAAATATCCAGAATT
TTACACATCAACAGAGCATAAACATTGACATGAGCCAATCCTGGGTAGGAATGTGACTTCACAGCTTACAAAAACAATCTGCCATGCTTTTCAGATATGTAGTTCTATGTTCTTTAACAATTGGATGAGGATTCTTGGAAATTTGAAATGTAGCATAGCTGTTGCTAAAGTCTGCAGAAAATCCACCTCCATGTCTTTTC
AATTACATTGAGTGCATATATAGCATCCGCTTAACCACAAAACAAAATTAGCCTGACAAGTTGACTACAATCAACTGCCTGTCTGTCTTACACCTATGTCACTCTTACATAATGATGCAAGAAAACATCAAAAGAGGGCTTCTTTAGTTGCAAGAAACCAGCGTCAACCAAATCACTAAAGTTCTGGCATAGTTGTGAAA
AAACATACAAGACTGAGTGATATTCCACAATGGTTGGGAAGTCTAAAAAGAAACTAGCAAAGGAAAATAAAGGAAAGGGAAATTGGAAAACTGGGTTGTGTATGCCTTCTAGATAACATATTGAGAACATGATTATTGGTTGTTTCCTTCAAGCCCTTGAAAGCATGGCTTCAAGATCTTTGCATTCCAATAAAATCCTT
AAGTCTTCTAGATTTTTAAGTAGTTTTTGATGCATTCATTTTACATGCCCAATTGTATTTGGGCCAATTTTCGGAAAGTTTCTAAATCTTTGCATTTAAAGTGAAGATTTACATTTTACATTAGCAATTGCATTGGACCCTCCATGGATGCTCTCGAAAATCTCTCATATGGGTCTTAGCTCTTCCAAACAACGTGACTT
TACAAATCCGTCTCTAAAAATGGATGAGAATTATGTTATCCCAACAAGTCATCCTCCACCCATATAAGTTTTTATTTATCGTATCTCATTTCGGTAATTAGCCAGATATCTAAGTGCATAATCCATTTTGTTGGAACTTATAGCAGACATTGTTGGACGAGGGTTCCATGATATTGATACCGGGGGCACCTACAGGCTCA
TTTGCGTATATATGTAAAGGCCCCGGAATATGAAATGGTTTATTGTTGGATATTAGCTTGTTGGTAGTTTTTTATTATTGGTTTTGGGTTGTTCACTATTGTGTTATAAGACTGTGAAATGTATGCCATTTATGAGCTCTAAACTTATGATGTAATGTTGTTGTGCCTTGAGTAATGTTGGGCATATGCAATGATGTATT
AGACCCTGTTGGAAATGAATGGAATGATGCCCCTTTTCCTACTACAATTTTTGGATTCTGCCATTTCATTCACCTAGCCCTGGGTGCCTCCAATGC

>TRINITY_DN125687_c3_g1_i3 from sample A2 / Example unspliced isoform:

TATTTAGGACAGGGTATTACatgctgggtggccaaaaaaaaaatagttatgtaggctggcaatataaaacATGATGCTTAGAAGCTATTTAGGACAGGGTATTACATGTTAGCATCCCGTGATATGAACCCCTGAATTACCTTAGAGATAAGTTCTATCCAAAAGGTTTTGGTCAGTTCTGGCTGACCACAAGTTAAGAA
TTCAAGGCTATTGAAATTCCTCTTTGACCAACCTTTTACATCCACATTATTGGATGGTTCAACAGTTTCATGACGACTTTGCCAGTATCTATATTTCACAATGTGAGTCTCACGCAATGCAAACGATGATCACGTCCACATCCCAAAATATTTGTACAGTAAATCCCGAGAACAAAGTAGGGAATTCTTGATTCAAAAGA
TGTTGCCAATATTCAGGAGAAGACCAAATATCCAGAATTTTACACATCAACAGAGCATAAACATTGACATGAGCCAATCCTGGGTAGGAATGTGACTTCACAGCTTACAAAAACAATCTGCCATGCTTTTCAGATATGTAGTTCTATGTTCTTTAACAATTGGATGAGGATTCTTGGAAATTTGAAATGTAGCATAGCTG
TTGCTAAAGTCTGCAGAAAATCCACCTCCATGTCTTTTCAATTACATTGAGTGCATATATAGCATCCGCTTAACCACAAAACAAAATTAGCCTGACAAGTTGACTACAATCAACTGCCTGTCTGTCTTACACCTATGTCACTCTTACATAATGATGCAAGAAAACATCAAAAGAGGGCTTCTTTAGTTGCAAGAAACCAG
CGTCAACCAAATCACTAAAGTTCTGGCATAGTTGTGAAAAAACATACAAGACTGAGTGATATTCCACAATGGTTGGGAAGTCTAAAAAGAAACTAGCAAAGGAAAATAAAGGAAAGGGAAATTGGAAAACTGGGTTGTGTATGCCTTCTAGATAACATATTGAGAACATGATTATTGGTTGTTTCCTTCAAGCCCTTGAA
AGCATGGCTTCAAGATCTTTGCATTCCAATAAAATCCTTAAGTCTTCTAGATTTTTAAGTAGTTTTTGATGCATTCATTTTACATGCCCAATTGTATTTGGGCCAATTTTCGGAAAGTTTCTAAATCTTTGCATTTAAAGTGAAGATTTACATTTTACATTAGCAATTGCATTGGACCCTCCATGGATGCTCTCGAAAAT
CTCTCATATGGGTCTTAGCTCTTCCAAACAACGTGACTTTACAAATCCGTCTCTAAAAATGGATGAGAATTATGTTATCCCAACAAGTCATCCTCCACCCATATAAGTTTTTATTTATCGTATCTCATTTCGGTAATTAGCCAGATATCTAAGTGCATAATCCATTTTGTTGGAACTTATAGCAGACATTGTTGGACGAG
GGTTCCATGATATTGATACCGGGGGCACCTACAGGCTCATTTGCGTATATATGTAAAGGCCCCGGAATATGAAATGGTTTATTGTTGGATATTAGCTTGTTGGTAGTTTTTTATTATTGGTTTTGGGTTGTTCACTATTGTGTTATAAGACTGTGAAATGTATGCCATTTATGAGCTCTAAACTTATGATGTAATGTTGT
TGTGCCTTGAGTAATGTTGGGCATATGCAATGATGTATTAGACCCTGTTGGAAATGAATGGAATGATGCCCCTTTTCCTACTACAATTTTTGGATTCTGCCATTTCATTCACCTAGCCCTGGGTGCCTCCAATGC