sp|Q949Q5|PSAO_ARATH ----> Photosystem I subunit O

Intron 1 GTTGATACCC ... TGTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.09	16	3.4	2.4
A2	45.55	5	4.2	2.2
A3	5	12	0.8	2.4
A4	48.68	7	6.8	1.2
A5	23.29	9	3	1.6
A6	8.89	183	19	34
A7	8.09	54	5	11.2
A8	5	27	1.8	5.4
A9	7.52	66	6.2	11.4
A10	6.64	33	2.6	5.2

>TRINITY_DN96779_c0_g1_i3 from sample A2 / Example spliced isoform

TGAATTTGCTATTGGCTTCGGGTACGTCTCACCTCTCATCGACAGAGGACTGCATGCATCAATGGCAACAATGGCAGTCGCAGCAGCAAACGTGACACTCAGATCTCCAGTTGCAGGCCTTGGCACTTCAACTCTGTCGCCATCAAAGCCCAGTGCTAAGCTCCAACTATCCTCTGGCTTTACAGGACGAACCACCGTGA
GAGGGAGTAATCCTCTGAGACAGAAGGGTCTTTGGGCTTCAAGGATTACATGCTTCCAGAGGAACTGGCTGAGGACAGACTATTCAGTGATAGGCTTTGGCCTCATTGCGTGGATTGCTCCTTCAAGCATTCCAGCCATAAATGGAGATAGTTTGACTGGGCTTTTCTTTAAAAGCATCGGTGAGGAGCTCGCCCACTTC
CCTACAGGCCCTGCACTCACATCTCCTTTCTGGTTGTGGTTGATTTGTTGGCACCTGGGATTGTTTATATGTCTGACGTTTGGACAAATTGGTTTCAAGGGAAGGAGTGAATATTTCTAAGCATGCGATTCCTAATCCCAGCTCTTGAAGACTGGAGTCTCTTATTCTAATTTTATTTCTGTCCAAGACAAACTTTTGTG
GGTATTTATAATGCTGCTCTTATGTGTTGTATGATTTTCCCTTTTCCCTTTCTGGTAATATAATCTTCAAAATCTTTTTTTTTTGTTTGTGTGTAAAGGTATGGCG

>TRINITY_DN96779_c0_g1_i2 from sample A2 / Example unspliced isoform:

TGAATTTGCTATTGGCTTCGGGTACGTCTCACCTCTCATCGACAGAGGACTGCATGCATCAATGGCAACAATGGCAGTCGCAGCAGCAAACGTGACACTCAGATCTCCAGTTGCAGGCCTTGGCACTTCAACTCTGTCGCCATCAAAGCCCAGTGCTAAGCTCCAACTATCCTCTGGTATGAATTCACTCACTATACTAC
TAACCTTCAAAGAACTCGCTCTCATGCTGTTTTCTGGTTAGTGATTTGCATTGCATGATTTCGATAGGGTAGTCTTAAACTTCTACAGCAATTTGTGTTTGGTGAATTATGTTTGTATGGACGCCAGGCTTTACAGGACGAACCACCGTGAGAGGGAGTAATCCTCTGAGACAGAAGGGTCTTTGGGCTTCAAGGATTAC
ATGgttgatacccatcactcaatatcgtagtcaaatacgcaatttttctctgttattgaagcacccaattgtcgttagtaataaaagctgtaataataatattgttgtacagCTTCCAGAGGAACTGGCTGAGGACAGACTATTCAGTGATAGGCTTTGGCCTCATTGCGTGGATTGCTCCTTCAAGCATTCCAGCCATA
AATGGAGATAGTTTGACTGGGCTTTTCTTTAAAAGCATCGGTGAGGAGCTCGCCCACTTCCCTACAGGCCCTGCACTCACATCTCCTTTCTGGTAACCATTTCCAATCCCAAC

Intron 2 GTAACCATTT ... TGGCCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	4	0	3
A2	34.62	17	3.5	9
A3	10.53	17	4.5	2
A4	15.38	11	5	2
A5	12.5	7	1	1
A6	17.43	208.5	28	44
A7	22.39	52	5	15
A8	19.23	42	4	10
A9	16	84	13.5	16
A10	17.65	28	4.5	6

>TRINITY_DN34668_c0_g1_i3 from sample A6 / Example spliced isoform

AGGAATGAATGAAACTAACCTTATCTACATCATCCAAAATCTAACCTCCCCATTGGCTCCTCTCGTCATCTATCACCTCAAGCGCTAGATGACTGGCTGAGAATGTCGGGACACCCCATCCATTCATCATCGCGATTTGGTGAATTTGCTATTGGCTTCGGGTACGTCTCACCTCTCATCGACAGAGGACTGCATGCATC
AATGGCAACAATGGCAGTCGCAGCAGCAAACGTGACACTCAGATCTCCAGTTGCAGGCCTTGGCACTTCAACTCTGTCGCCATCAAAGCCCAGTGCTAAGCTCCAACTATCCTCTGGCTTTACAGGACGAACCACCGTGAGAGGGAGTAATCCTCTGAGACAGAAGGGTCTTTGGGCTTCAAGGATTACATGCTTCCAGA
GGAACTGGCTGAGGACAGACTATTCAGTGATAGGCTTTGGCCTCATTGCGTGGATTGCTCCTTCAAGCATTCCAGCCATAAATGGAGATAGTTTGACTGGGCTTTTCTTTAAAAGCATCGGTGAGGAGCTCGCCCACTTCCCTACAGGCCCTGCACTCACATCTCCTTTCTGGTTGTGGTTGATTTGTTGGCACCTGGGA
TTGTTTATATGTCTGACGTTTGGACAAATTGGTTTCAAGGGAAGGAGTGAATATTTCTAAGCATGCGATTCCTAATCCCAGCTCTTGAAGACTGGAGTCTCTTATTCTAATTTTATTTCTGTCCAAGACAAACTTTTGTGGGTATTTATAATGCTGCTCTTATGTGTTGTATGATTTTCCCTTTTCCCTTTCTGGTAATA
TAATCTTCAAAATCTTTTTTTTTTGTTTGTGTGTAAAGGTATGGCGATGTTGTTAAACAACCTGACTCTTACTTTCAATGTTGGTCTTCCTTGTTTTGGTTAACTATCCAGAATAGCACAGGTGCAATTCACAAATTCCCTGTGCATATTAGTGTTACTTTTCACATACAATTGCAGCAATTAATTAAGGCCATAAATGG
TTGTAATCCATGGATTTTTGAAAGTTGAGCTTAGTGGCAGTAGACAACTGCAGGGTTGCATTTTTTTCTCAACTACAAGAACTCTCTTAAACACTGAAAAATGGCTATTACTCTAGTTGCAAGGTGTATATAGCCTAGCTCCTGCTCCATTATGTAATGGTATACTGCTTTAAAAGGCTGTAGGCATACATAAATCCAGT
CACCATCTGTAAATTCTTGCTCAAATTGATATTTATGTATTTGTAACTTCATATGATTTAGGCCTTTGCCAAACTCTCCTTCAAATCTTCCATGGCATAAC

>TRINITY_DN34668_c0_g1_i2 from sample A6 / Example unspliced isoform:

AGGAATGAATGAAACTAACCTTATCTACATCATCCAAAATCTAACCTCCCCATTGGCTCCTCTCGTCATCTATCACCTCAAGCGCTAGATGACTGGCTGAGAATGTCGGGACACCCCATCCATTCATCATCGCGATTTGGTGAATTTGCTATTGGCTTCGGGTACGTCTCACCTCTCATCGACAGAGGACTGCATGCATC
AATGGCAACAATGGCAGTCGCAGCAGCAAACGTGACACTCAGATCTCCAGTTGCAGGCCTTGGCACTTCAACTCTGTCGCCATCAAAGCCCAGTGCTAAGCTCCAACTATCCTCTGGCTTTACAGGACGAACCACCGTGAGAGGGAGTAATCCTCTGAGACAGAAGGGTCTTTGGGCTTCAAGGATTACATGCTTCCAGA
GGAACTGGCTGAGGACAGACTATTCAGTGATAGGCTTTGGCCTCATTGCGTGGATTGCTCCTTCAAGCATTCCAGCCATAAATGGAGATAGTTTGACTGGGCTTTTCTTTAAAAGCATCGGTGAGGAGCTCGCCCACTTCCCTACAGGCCCTGCACTCACATCTCCTTTCTGgtaaccatttccaatcccaacttcatat
tatttcataattttgtatagattcatcttgacacactttgtatagatccatcttaactatcatattattagatttaaaaaaaatagataaaaatattttgatgcttaaaaaacagaggccaggtgctgttagatggaaggaatgaatgtgtagtttagtgaagtattcagagatttggctctttataacttaggcctggt
ttgatttggccaacagGTTGTGGTTGATTTGTTGGCACCTGGGATTGTTTATATGTCTGACGTTTGGACAAATTGGTTTCAAGGGAAGGAGTGAATATTTCTAAGCATGCGATTCCTAATCCCAGCTCTTGAAGACTGGAGTCTCTTATTCTAATTTTATTTCTGTCCAAGACAAACTTTTGTGGGTATTTATAATGCTG
CTCTTATGTGTTGTATGATTTTCCCTTTTCCCTTTCTGGTAATATAATCTTCAAAATCTTTTTTTTTTGTTTGTGTGTAAAGGTATGGCGATGTTGTTAAACAACCTGACTCTTACTTTCAATGTTGGTCTTCCTTGTTTTGGTTAACTATCCAGAATAGCACAGGTGCAATTCACAAATTCCCTGTGCATATTAGTGTT
ACTTTTCACATACAATTGCAGCAATTAATTAAGGCCATAAATGGTTGTAATCCATGGATTTTTGAAAGTTGAGCTTAGTGGCAGTAGACAACTGCAGGGTTGCATTTTTTTCTCAACTACAAGAACTCTCTTAAACACTGAAAAATGGCTATTACTCTAGTTGCAAGGTGTATATAGCCTAGCTCCTGCTCCATTATGTA
ATGGTATACTGCTTTAAAAGGCTGTAGGCATACATAAATCCAGTCACCATCTGTAAATTCTTGCTCAAATTGATATTTATGTATTTGTAACTTCATATGATTTAGGCCTTTGCCAAACTCTCCTTCAAATCTTCCATGGCATAAC

Intron 3 GTATGAATTC ... TGGACGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.82	14	4.5	2
A2	20.69	17	6	3
A3	17.24	19	5	1
A4	12.95	13	2	0
A5	19.05	13	4	1
A6	8.73	222	23.5	6
A7	11.43	54	8	4
A8	17.52	30	7.5	0
A9	7.5	85	7.5	1
A10	9.57	38	4.5	0

>TRINITY_DN96779_c0_g1_i3 from sample A2 / Example spliced isoform

TGAATTTGCTATTGGCTTCGGGTACGTCTCACCTCTCATCGACAGAGGACTGCATGCATCAATGGCAACAATGGCAGTCGCAGCAGCAAACGTGACACTCAGATCTCCAGTTGCAGGCCTTGGCACTTCAACTCTGTCGCCATCAAAGCCCAGTGCTAAGCTCCAACTATCCTCTGGCTTTACAGGACGAACCACCGTGA
GAGGGAGTAATCCTCTGAGACAGAAGGGTCTTTGGGCTTCAAGGATTACATGCTTCCAGAGGAACTGGCTGAGGACAGACTATTCAGTGATAGGCTTTGGCCTCATTGCGTGGATTGCTCCTTCAAGCATTCCAGCCATAAATGGAGATAGTTTGACTGGGCTTTTCTTTAAAAGCATCGGTGAGGAGCTCGCCCACTTC
CCTACAGGCCCTGCACTCACATCTCCTTTCTGGTTGTGGTTGATTTGTTGGCACCTGGGATTGTTTATATGTCTGACGTTTGGACAAATTGGTTTCAAGGGAAGGAGTGAATATTTCTAAGCATGCGATTCCTAATCCCAGCTCTTGAAGACTGGAGTCTCTTATTCTAATTTTATTTCTGTCCAAGACAAACTTTTGTG
GGTATTTATAATGCTGCTCTTATGTGTTGTATGATTTTCCCTTTTCCCTTTCTGGTAATATAATCTTCAAAATCTTTTTTTTTTGTTTGTGTGTAAAGGTATGGCG

>TRINITY_DN96779_c0_g1_i2 from sample A2 / Example unspliced isoform:

TGAATTTGCTATTGGCTTCGGGTACGTCTCACCTCTCATCGACAGAGGACTGCATGCATCAATGGCAACAATGGCAGTCGCAGCAGCAAACGTGACACTCAGATCTCCAGTTGCAGGCCTTGGCACTTCAACTCTGTCGCCATCAAAGCCCAGTGCTAAGCTCCAACTATCCTCTGgtatgaattcactcactatactac
taaccttcaaagaactcgctctcatgctgttttctggttagtgatttgcattgcatgatttcgatagggtagtcttaaacttctacagcaatttgtgtttggtgaattatgtttgtatggacgccagGCTTTACAGGACGAACCACCGTGAGAGGGAGTAATCCTCTGAGACAGAAGGGTCTTTGGGCTTCAAGGATTAC
ATGGTTGATACCCATCACTCAATATCGTAGTCAAATACGCAATTTTTCTCTGTTATTGAAGCACCCAATTGTCGTTAGTAATAAAAGCTGTAATAATAATATTGTTGTACAGCTTCCAGAGGAACTGGCTGAGGACAGACTATTCAGTGATAGGCTTTGGCCTCATTGCGTGGATTGCTCCTTCAAGCATTCCAGCCATA
AATGGAGATAGTTTGACTGGGCTTTTCTTTAAAAGCATCGGTGAGGAGCTCGCCCACTTCCCTACAGGCCCTGCACTCACATCTCCTTTCTGGTAACCATTTCCAATCCCAAC