sp|Q9CAF4|NUP1_ARATH ----> Nuclear pore complex protein NUP1

Intron 1 GTATGTAAAC ... ATGAACCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.1	53.8	22	24
A2	28.5	31.3	12.4	14.1
A3	32.69	47.8	23.2	10.7
A4	43.52	28.6	22	15.4
A5	11.59	30.6	4	9.8
A6	50.96	31.8	33	10.9
A7	41.6	47.8	34	20.2
A8	49.31	52	50.5	14.5
A9	32.23	44.2	21	4.6
A10	34.23	51.9	27	15

>TRINITY_DN118690_c0_g1_i15 from sample A1 / Example spliced isoform

AAAAAAAACGAGCGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCG
AGGACCCCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGG
AGACGAGGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACC
TGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTC
TCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAA
CAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACAC
CATTCACGGGATTTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCC
CAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTC
TTCTGTTAAGGGCAGTTTGGATGTTCAATCCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTA
ATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTGTACTGTGCCTTCACACTCAAGTCTAACAGCCC
AGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTG
CAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTT
TCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATC
ATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTCCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATA
GCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACA
TCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGTCATCCGGTCCTGTACCATCAGTCTTTGTTCA
ACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTC
GGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTAC
AATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTT
GCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAA
GGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACTGTAAGCAAATCGTGTTTCTGGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACAGGAAAAAAAAATTATTAATGGAACGGATTCGGAATAAATGTAAAAATGCTTATTTAC
TCTTTTGGAGCAAATCTCACTTTTTGGCTACTGCAAGG

>TRINITY_DN118690_c0_g1_i18 from sample A1 / Example unspliced isoform:

AAAAAAAACGAGCGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCG
AGGACCCCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGG
AGACGAGGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACC
TGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTC
TCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAA
CAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACAC
CATTCACGGGATTTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCC
CAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTC
TTCTGTTAAGGGCAGTTTGGATGTTCAATCCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAgtatgtaaacaggaggcatgcgatatggaaactgagttaaaccataaataaaactatctcttctattgcagtttagagaaaagaatagagagctcagaaagag
aatgatttgaggtttttaattttatgcaattttcttaacaatttgtaatttttttattatttgctgagctcctgcatgaatgaacccagGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACT
CCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATT
CAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACA
AAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTT
TATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATAT
TTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTCCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGT
ACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCC
TTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTT
CATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTC
ACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTG
CAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTAT
CATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGC

Intron 2 GTAATTTTCC ... GCATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.34	53.1	8	12.7
A2	21.42	25.7	5	7
A3	27.79	36.6	1	14
A4	25.04	38	2	12.7
A5	21.82	22.6	5	6.3
A6	30.01	35.1	2	15
A7	17.4	58.4	3	12.3
A8	23.17	52.1	8	15.7
A9	24.84	48.7	0	16
A10	31.8	40.8	1	19

>TRINITY_DN43101_c0_g1_i7 from sample A6 / Example spliced isoform

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATGTAAGTGCTCTGGAGAGTACAAATTTAAAAATGCACTGCTATCTTTAGATGCAGAGTCTTGAAGCTTAGACCAGTAATCAATATATGCTTGTACA
GTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATCCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCC
ATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTT
GATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACA
GAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTG
GCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCA
AGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAAC
TCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAA
ATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAG
AAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCT
GTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATAT
GCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACTGTAAGCAAATCGTGTTTCTGGTGTGCAACGGAGCAGGAAATG
TGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACAGGGAAAAAAAATTATTAATGGAACGGATTCGGAATAAATGTAAAAATGCTTATTTACTCTTTTGGAGCAAATCTCACTTTTTGGCTACTGCAAGGACAACTATGATCAATTGTTAAAAATCATTGTCGTTTTCTTCTTACAAACTAAGTTTGAGTAATA
GTCTATGAATTGTTTGATGTACTATGCAAAAGGGAAACTGGATATTGGATAAACATGTGGGAAATAAAAAATTTACACGGCCATTTCTTTGGAGGTCAAAATTGGGAAAAATTGCACATCATCCGCAATGTTAAACTGTGGAGAAGTATTAAACCTCCAAAAGCAGGGC

>TRINITY_DN43101_c0_g1_i2 from sample A6 / Example unspliced isoform:

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATGTAAGTGCTCTGGAGAGTACAAATTTAAAAATGCACTGCTATCTTTAGATGCAGAGTCTTGAAGCTTAGACCAGTAATCAATATATGCTTGTACA
GTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATCCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGTATGTAAACAGGAGGCAT
GCGATATGGAAACTGAGTTAAACCATAAATAAAACTATCTCTTCTATTGCAGTTTAGAGAAAAGAATAGAGAGCTCAGAAAGAGAATGATTTGAGGTTTTTAATTTTATGCAATTTTCTTAACAATTTGTAATTTTTTTATTATTTGCTGAGCTCCTGCATGAATGAACCCAGGGTTTAGAAAACGATGTCCATGGAAGC
AAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGgtaattttcctttatactaaagctccccaaacaaataagccattagttaaattttttacggtaatcttctgcctagtccgtttggcagaaatagaaattatggtatcttctgccaagtcagtttggcagaaatagaaac
tatggccttcactttggttgccattttagttttgaatctgttgctgttggcagatgtatgtttccccagttgtgttactagtttctactcatttagaatcactactgactcgcttatttctgtgcatttgcagTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGT
TTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCA
TGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCC
TCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCC
ACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGA
CACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACT
CTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAG
GAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTA
ATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGT
AAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTA
AAAACCCAAGGCAGTCGGGAACTGTGAGCAAATCGTGTTTCTAGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACGGAAAAAAAATTATAAATGGAACGATTCGGAATAAATGTAAAAATGCTTGGTTACTCTTTTGGAGCAAATCTCACTTTTTTGGCTACTGCAAGGA
CAACTATGATCAATTGTTAAAAATCATTGTCGTTTTCTTCTTACAAACTAAGTTTGAGTAATAGTCTATGAATTGTTTGATGTACTATGCAAAAGGGAAACTGGATATTGGATAAACATGTGGGAAATAAAAAATTTACACGGCCATTTCTTTGGAGGTCAAAATTGGGAAAAATTGCACATCATCCGCAATGTTAAACT
GTGGAGAAGTATTAAACCTCCAAAAGCAGGGC

Intron 3 GTAAGTGCTC ... GCTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.08	44.5	28	16
A2	31.91	32	13	17
A3	22.39	26	10	5
A4	30.34	31	14	13
A5	25	3	1	1
A6	27.27	28	10	11
A7	47.83	24	25	19
A8	46.05	41	36	34
A9	35.06	25	15	12
A10	26.92	19	6	8

>TRINITY_DN118690_c0_g1_i15 from sample A1 / Example spliced isoform

AAAAAAAACGAGCGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCG
AGGACCCCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGG
AGACGAGGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACC
TGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTC
TCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAA
CAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACAC
CATTCACGGGATTTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCC
CAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTC
TTCTGTTAAGGGCAGTTTGGATGTTCAATCCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTA
ATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTGTACTGTGCCTTCACACTCAAGTCTAACAGCCC
AGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTG
CAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTT
TCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATC
ATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTCCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATA
GCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACA
TCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGTCATCCGGTCCTGTACCATCAGTCTTTGTTCA
ACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTC
GGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTAC
AATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTT
GCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAA
GGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACTGTAAGCAAATCGTGTTTCTGGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACAGGAAAAAAAAATTATTAATGGAACGGATTCGGAATAAATGTAAAAATGCTTATTTAC
TCTTTTGGAGCAAATCTCACTTTTTGGCTACTGCAAGG

>TRINITY_DN118690_c0_g1_i16 from sample A1 / Example unspliced isoform:

AAAAAAAACGAGCGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCG
AGGACCCCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGG
AGACGAGGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACC
TGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTC
TCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAA
CAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACAC
CATTCACGGGATTTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCC
CAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATgtaagtgctctggagagtacaaatttaaaaatgcactgctatctttagatgcagagtcttgaagcttagaccagtaatcaata
tatgcttgtacagTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATCCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAG
AAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTT
AATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATG
AATCTAATAAATTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCT
ACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTT
TGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCT
CAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTCCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGT
AATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCC
ACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATT
TATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAG
ACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTA
TCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCA
TTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACTGTAAGCAAATCGTGTTTCTGGTGTGCAACGGAGCAGGAAATGTG
CGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACAGGAAAAAAAAATTATTAATGGAACGGATTCGGAATAAATGTAAAAATGCTTATTTACTCTTTTGGAGCAAATCTCACTTTTTGGCTACTGCAAGG

Intron 4 GTTAGTCCTC ... GGTATGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.67	52	7	23
A2	36.67	38	2	22
A3	31.88	47	4	22
A4	23.81	32	7	10
A5	31.43	24	2	11
A6	32.79	41	8	20
A7	25.77	72	7	25
A8	32.69	70	13	34
A9	30.39	71	13	31
A10	18.18	54	3	12

>TRINITY_DN113844_c0_g2_i12 from sample A3 / Example spliced isoform

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGG
CAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAA
CATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATT
TCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGG
ATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTC
ATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGAT
ACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGT
TCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATA
GTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTG
TACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGC
TTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCT
AAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACTGTAAGCAAATCGTGTTTCTGGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACGGAAAAAAAATTATAAATGGAACGATTCGG

>TRINITY_DN113844_c0_g2_i5 from sample A3 / Example unspliced isoform:

TATCTTCTGCCAAGTCAGTTTGGCAGAAATAGAAACTATGGCCTTCACTTTGGTTGCCATTTTAGTTTTGAATCTGTTGCTGTTGGCAGATGTATGTTTCCCCAGTTGTGTTACTAGTTTCTACTCATTTAGAATCACTACTGACTCGCATATTTCTGTGCATTTGCAGTTAGCCTACAGTGTAATGCAACTCCAACATT
CAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGgttagtcctcattttgttgatttcattgccccttgcttgagttgccattaagtcagcttttgcagggcttatgt
tttatcttgctgctgggtatgtcttttttaattttgacgagggtatgtcagTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCC
CTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAG
TCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCT
ATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAG
CGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAG
GCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGT
TGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAG

Intron 5 GTAGATCTCA ... TTTATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	88	6	44	21
A2	83.33	12	60	12
A3	72.22	20	52	8
A4	68.97	9	20	10
A5	73.08	7	19	3
A6	74.52	67	196	43
A7	77.4	66	226	51
A8	75.95	120	379	75
A9	76.68	80	263	81
A10	76.34	44	142	19

>TRINITY_DN113844_c0_g2_i6 from sample A3 / Example spliced isoform

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGG
CAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAA
CATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATT
TCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGG
ATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTC
ATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGAT
ACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGT
TCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATA
GTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGTGGCAGCTTTGCATTTATCATGGAAACT
CCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGC

>TRINITY_DN113844_c0_g2_i13 from sample A3 / Example unspliced isoform:

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGG
CAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAA
CATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATT
TCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGG
ATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTC
ATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGAT
ACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGT
TCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATA
GTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGgtagatctcaagagtctagctgattttg
tacaattactgcaattttgaattgctgtaaaagatttttaatgtgcttgtggagtgtaagatatgcagaacatgcgtctctgggtatgcatgtaattcatgtctgtatcatagtttgttgcttcataatgtatttccaaactacttgatgtgcctgaatccgatagaaaggtctgaccagcagtttattttagtggcagc
tttgcatttatcatggaaactccctaaacttagacctggtaaacagttgtttattttagTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACC
TAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCCAAGGCAGTCGGGAACTGTGAGCAAATCGTGTTTCTAGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACGGAAAAAAAATTATAAATGGAACGATTCGG

Intron 6 GTAAGTGCTC ... ATGAACCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.8	35	28	24.9
A2	34.51	26	13	15
A3	39.27	16	10	10.9
A4	46.55	17	14	16.1
A5	32.68	11	1	10.4
A6	33.51	21	10	11.7
A7	42.38	31	25	20.8
A8	54.61	21	36	14.6
A9	23.78	32	15	5.1
A10	36.85	18	6	15.4

>TRINITY_DN110731_c0_g1_i6 from sample A2 / Example spliced isoform

AACGAGCGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACC
CCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCAACGACAGGAATCGGCAGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGA
GGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTG
GGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGG
CTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGAT
GCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGCTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCA
CGGGATTTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAA
ATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGT
TAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCA
ATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTT
ACATCAGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTT
GGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTCCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTG
GTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCA
ACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGTCATCCGGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGG
TGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCC
TATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTTTGCATGTAATTCATGTCTGTATCATAGTTTGTTG
CTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCG
CACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCCAAGGCAGTCGGGAACTGTGAGCAAATCGTGTTTCTAGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTA
CTCTACCCTTTTCTCGCACAAGGACGGAAAAAAAATTATAAATGGAACGATTCGGAATAAATGTAAAAATGCTTGGTTACTCTTTTGGAGCAAATCTCACTTTTTTGGCTACTGCAAGGACAACTATGATCAATTGTTAAAAATCATTGTCGTTTTCTTCTTAC

>TRINITY_DN110731_c0_g1_i1 from sample A2 / Example unspliced isoform:

AACGAGCGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACC
CCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCAACGACAGGAATCGGCAGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGA
GGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTG
GGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGG
CTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGAT
GCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGCTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCA
CGGGATTTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAA
ATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATgtaagtgctctggagagtacaaatttaaaaatgcactgctatctttagatgcagagtcttgaagcttagaccagtaatcaatatatgct
tgtacagtgtgactcagatgtgcagaacaattacaaggacaatggtaatactcccttgttaacggcaacatctctggagaggcctagttcttctgttaagggcagtttggatgttcaatccattagaccagaagcaaacaaggctgacttagctgatggttctttatttgaaaagaggaaagatcaagtatgtaaacagg
aggcatgcgatatggaaactgagttaaaccataaataaaactatctcttctattgcagtttagagaaaagaatagagagctcagaaagagaatgatttgaggtttttaattttatgcaattttcttaacaatttgtaatttttttattatttgctgagctcctgcatgaatgaacccagGGTTTAGAAAACGATGTCCAT
GGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGGTAATTTTCCTTTATACTAAAGCTCCCCAAACAAATAAGCCATTAGTTAAATTTTTTACGGTAATCTTCTG

Intron 7 GTAAGGACAT ... CGTATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.19	132.6	3.8	49
A2	26.71	110.6	3	40
A3	24	116	5.6	36
A4	29.35	99.6	4	41
A5	24.6	89.2	5.4	29
A6	29.87	61.4	0.8	26
A7	25.21	134.8	4.4	45
A8	26.45	65.2	4	23
A9	27.74	117	6.4	44
A10	27.86	116.6	6.2	44

>TRINITY_DN102711_c0_g2_i10 from sample A4 / Example spliced isoform

GCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATATGACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCAACGACAGGAATCGGCAGAGACTTCTTCTT
CTTACTCTTCTTCAACGGACGACGGAGACGAGGACAGTTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACA
TTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATG
TGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGAT
CTCCAACACCAGCAATGATGTCGAACAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGCTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTT
GGAAATGTTTCATCATTTTCCACACCATTCACGGGATTTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGT
GCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATGTAAGTGCTCTGGAGAGTACAAATTTAAAAATGCACTGCTATCTTTAGATGCAGAGTC
TTGAAGCTTAGACCAGTAATCAATATATGCTTGTACAGTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTT
GAAAAGAGGAAAGATCAAGTATGTAAACAGGAGGCATGCGATATGGAAACTGAGTTAAACCATAAATAAAACTATCTCTTCTATTGCAGTTTAGAGAAAAGAATAGAGAGCTCAGAAAGAGAATGATTTGAGGTTTTTAATTTTATGCAATTTTCTTAACAATTTGTAATTTTTTTATTATTTGCTGAGCTCCTGCATGA
ATGAACCCAGGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAATTAGATA
CTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCA
ACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACC
CTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTCCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTT
GTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAA
TTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACG

>TRINITY_DN102711_c0_g2_i4 from sample A4 / Example unspliced isoform:

TTCAAGTAGTGGCAGCAGTGGAGgtaaggacattttgtctgattgatttgtattttaccacttacagcaatggactgcttcagagaaatattggaaacagttgtcttggcttgtatacatttaaaattaaaattgagatacattacaacttttggtggagaaggtacacatggatctttgatttgaaagcttttaggata
atttgtgtttgaacctcttattttctagggaagacttatagcgcttccgtattgcagAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGTGACATGCATTCTCATTCAATTT
TTTCTTGATATTGTAGAATTAAATTAGATATCTTGCACTTATTTGCGTTTTTTTGTGGATTCTTTTTCTCGGAAACAGATTTTATATGTCTTATATCTTGTGAACTCGTTTATTTTGTGGCCTTTTGAGTGCCAAAGTTCTGCCC

Intron 8 GTCCTGTTTT ... GGTTATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.53	28	41.6	52
A2	51.18	26	16.6	38
A3	54.53	32	29.8	47
A4	61.95	19	30.2	32
A5	60.61	12	13	24
A6	60.86	24	33.8	41
A7	62.29	37	51	72
A8	58.43	44	54	70
A9	56.06	45	44	71
A10	73.81	17	41.8	54

>TRINITY_DN113844_c0_g2_i9 from sample A3 / Example spliced isoform

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGGGAAGACTTATAGCGCTTCCGTATTGCAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAG
GGATGAGTTCATTCACCTGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTG
GTTTGTGTGGCAATGTCTCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACA
CCAGCAATGATGTCGAACAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGT
TTCATCATTTTCCACACCATTCACGGGATTTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCAC
ACTCAAGTCTAACAGCCCAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCT
CTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGA
TGTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTAT
GTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAA
GGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGG
ACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAA
CACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACAC
AATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCAC
CGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAG

>TRINITY_DN113844_c0_g2_i13 from sample A3 / Example unspliced isoform:

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGG
CAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAA
CATTCAAGTTAGGCTCTGAAAATACTAGCACAGgtcctgttttatcatgtacaagtgcagctacaaaattagatacttccaagccgaattttaatttcaaatttgatagcaagcctgaaaggttattcagTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATT
TCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGG
ATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTC
ATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGAT
ACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGT
TCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATA
GTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTG
TACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGC
TTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTCAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACC
TAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAAAAACCCAAGGCAGTCGGGAACTGTGAGCAAATCGTGTTTCTAGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACGGAAAAAAAATTATAAATGGAACGATTCGG

Intron 9 GTCCTGTTTT ... GGTATGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.19	28	30	23
A2	39.08	26	11.5	22
A3	40	32	21	22
A4	48.61	19	26.5	10
A5	47.09	12	10.5	11
A6	49.27	24	27	20
A7	43.98	37	35	25
A8	45.86	44	41.5	34
A9	42.28	45	35.5	31
A10	52.25	17	25.5	12

>TRINITY_DN113844_c0_g2_i9 from sample A3 / Example spliced isoform

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGGGAAGACTTATAGCGCTTCCGTATTGCAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAG
GGATGAGTTCATTCACCTGACTGGGCTGCTACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTG
GTTTGTGTGGCAATGTCTCAAGGCTTGCTTTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACA
CCAGCAATGATGTCGAACAGGATGCAGAGCTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGT
TTCATCATTTTCCACACCATTCACGGGATTTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCAC
ACTCAAGTCTAACAGCCCAGAAAATTCAGGAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCT
CTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGGTTTAGAAAACGATGTCCATGGAAGCAAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGA
TGTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTAT
GTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAA
GGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGG
ACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAA
CACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACAC
AATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCAC
CGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAG

>TRINITY_DN113844_c0_g2_i4 from sample A3 / Example unspliced isoform:

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGCACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGACAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATGTAAGTGCTCTGGAGAGTACAAATTTAAAAATGCACTGCTATCTTTAGATGCAGAGTCTTGAAGCTTAGACCAGTAATCAATATATGCTTGTACA
GTGTGACTCAGATGTGCAGAACAATTACAAGGACAATGGTAATACTCCCTTGTTAACGGCAACATCTCTGGAGAGGCCTAGTTCTTCTGTTAAGGGCAGTTTGGATGTTCAATTCATTAGACCAGAAGCAAACAAGGCTGACTTAGCTGATGGTTCTTTATTTGAAAAGAGGAAAGATCAAGTATGTAAACAGGAGGCAT
GCGATATGGAAACTGAGTTAAACCATAAATAAAACTATCTCTTCTATTGCAGTTTAGAGAAAAGAATAGAGAGCTCAGAAAGAGAATGATTTGAGGTTTTTAATTTTATGCAATTTTCTTAACAATTTGTAATTTTTTTATTATTTGCTGAGCTCCTGCATGAATGAACCCAGGGTTTAGAAAACGATGTCCATGGAAGC
AAAAACGATACAATTCCCCTTGATGTTCAAACCTCAAAGTCTGTATCATCTGCATCAGATGTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGgtcctgttttatcatgtacaagtgcagctacaaaattagatacttccaagccgaattttaatttcaaatttgatagcaa
gcctgaaaggttattcaggttagtcctcattttgttgatttcattgccccttgcttgagttgccattaagtcagcttttgcagggcttatgttttatcttgctgctgggtatgtcttttttaattttgacgagggtatgtcagTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGAC
CTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTG
GTGCTTCATTTGGATCATCCATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGG
ACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATG
TAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTAC
CATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGC
AGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAACAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAG

Intron 10 GTAAGTGCTC ... GCATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.95	45	28	12.3
A2	41.67	14	13	7
A3	28.57	30	10	14
A4	45.14	16	14	12.3
A5	35.35	7	1	6.7
A6	37.31	21	10	15
A7	40.21	28	25	12.7
A8	40.96	37	36	15.3
A9	33.33	31	15	16
A10	25.25	37	6	19

>TRINITY_DN43101_c0_g1_i9 from sample A6 / Example spliced isoform

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGTTTTATCATGTACAAGTGCAGCTACAAAA
TTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCATGGCATCACAACCTGTTTCGCGGTTTAT
GTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCCTCAAAGACTTCATTTGGTTCAACGACAA
GGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCCACTTAAATCAAGTTCTGCGAGTTTTGGG
ACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGACACAGAAAACAATGTCTCTATCAACAAA
CACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACTCTTGCAGTTGATTCTCAGTCAAATACAC
AATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAGGAAGTAGAATGTAGCAGCTTGTAGGCAC
CGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTAATGTGCTTGTGGAGTGTAAGATATGCAG
AACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGTAAACAGTTGTTTATTTTAGTGGCAGCTT
TGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTT

>TRINITY_DN43101_c0_g1_i6 from sample A6 / Example unspliced isoform:

CGAAAGTTCCTTTTTCAATTTTCTTTCTGTTTCGTTTTGGTCATTTCTTTTTCTCGTCTAGGGTTTGTTCTTATTAGTCCACTGCTTGTTCGCAACAGACACCCGGAAACCCTAGAAAAATAGTTATGGACGAAGTGGGCGGCGCGGGGGGCAAGCAGCTCCGGCCCCGGAGGCGAGTCGTTTTTTCGAGGACCCCATAT
GACCGTCCGCTCGTGGGGCGGCATGCCCCTCCCGAGCTCAGAAACCCCAACAGTAACGGAGGCAGCAGCTGGTTTTCGAAGCTCCTTAGTCCGATAACAAATGGCGCCGGGAGGTTGCTCTCTTCAGTTTTCCGACGACAGGAATCGGCCGAGACTTCTTCTTCTTACTCTTCTTCAACGGACGACGGAGACGAGGACAG
TTCGGAGGAGGAGGAACAGGAAGATAATGTTCAAGTAGTGGCAGCAGTGGAGAATCGCATTGTAACAGAAGCACGTAAACAAAGTGGAGAACCTACTTCAGTAGGCAATAAGAAAAGTCAATCTAAACTTTTTGAAATTGAACAAGTACTTGCTGGCAAAACATTTACAAGGGATGAGTTCATTCACCTGACTGGGCTGC
TACAATCTCGAGTTGTAGATAGCTCTGGCTCCAGGGTGCATGAAGGGACAACAAGTACAGATCTACTCAGTGATACTCACCTGAGGCATGCGGCACTTGAGGAAACACGTAGATGGAGTGAAGAGAGAAGGAAAGAAAAGGAAGGAAGTGGGAATGGTGGATGTGATAGTGGTTTGTGTGGCAATGTCTCAAGGCTTGCT
TTGTCACCAGATGTTCTGCATGAGACAAGTTCTTCACCAGCAGACATTGCAAAAGCTTATATGGGAACACAGCATACTTGGTCATCCTCCCCTGTATCTTTCCAGTCTCACTTATCAAGAGATGAAGTTATACAGAATCGATGCTGGTCTGATACTTACAGATCTCCAACACCAGCAATGATGTCGAACAGGATGCAGAG
CTTAGCTTGTACTCCATACGCCATGGCTGATCATTTACAAACTCCATATTCTAGGGTTGCTTACTCTCAAAATTATTTCCAGATGCTCAAGAGGAGGAGCTCTTTGATAGATGACAGTTGGGCTTCAGCTGAAACAATTCGAAGAATACGACAAAAATCATTTGGAAATGTTTCATCATTTTCCACACCATTCACGGGAT
TTAATTCATCAATGATAGAAGCTGGACCAAGCATTGCTTGTTTATCATCAGGCCTTGAAGCAGGTCAAAACACATTTGACAAGAAGCCCATAGACACAGACAGAAGTAATAATGTTCTTCTATCCTCCCAAGTTAGAGAGGATGCACATCAAGTTTGTACTGTGCCTTCACACTCAAGTCTAACAGCCCAGAAAATTCAG
GAACATCTTGATGGAAAGCTGCCTCCAAATGAAAATTCTTCGGAAGAACAGTTAACCTTTGCAAGAGATAAGTCTTCAAGTCAGCCGACTATGAATCTAATAAATgtaagtgctctggagagtacaaatttaaaaatgcactgctatctttagatgcagagtcttgaagcttagaccagtaatcaatatatgcttgtaca
gtgtgactcagatgtgcagaacaattacaaggacaatggtaatactcccttgttaacggcaacatctctggagaggcctagttcttctgttaagggcagtttggatgttcaatccattagaccagaagcaaacaaggctgacttagctgatggttctttatttgaaaagaggaaagatcaagtatgtaaacaggaggcat
gcgatatggaaactgagttaaaccataaataaaactatctcttctattgcagtttagagaaaagaatagagagctcagaaagagaatgatttgaggtttttaattttatgcaattttcttaacaatttgtaatttttttattatttgctgagctcctgcatgaatgaacccagggtttagaaaacgatgtccatggaagc
aaaaacgatacaattccccttgatgttcaaacctcaaagtctgtatcatctgcatcagatggtaattttcctttatactaaagctccccaaacaaataagccattagttaaattttttacggtaatcttctgcctagtccgtttggcagaaatagaaattatggtatcttctgccaagtcagtttggcagaaatagaaac
tatggccttcactttggttgccattttagttttgaatctgttgctgttggcagatgtatgtttccccagttgtgttactagtttctactcatttagaatcactactgactcgcttatttctgtgcatttgcagTTAGCCTACAGTGTAATGCAACTCCAACATTCAAGTTAGGCTCTGAAAATACTAGCACAGGTCCTGT
TTTATCATGTACAAGTGCAGCTACAAAATTAGATACTTCCAAGCCGAATTTTAATTTCAAATTTGATAGCAAGCCTGAAAGGTTATTCAGTTCTGTTGCATTTGCTTCAAACTCTCAATCTACTTCAGTCAGCAATCATTTATCGACCTCTCAGTCAATTTCAGCAGGATCCATACATGGACTTGGCCCAAATTCTGTCA
TGGCATCACAACCTGTTTCGCGGTTTATGTTCTCAACCCCTTTATTTGCTGGCACTTCTGTACAGAGTACAGGCCCTTCAATTTTTGGCTCTGGTGCTTCATTTGGATCATCTTCAATTTTTGGCACACCTTTTACATCAGGCTCTGGTGCTTCATTTGGATCATCGATATTTGGCAAACCCTTTGCATTTGGTTCTGCC
TCAAAGACTTCATTTGGTTCAACGACAAGGACAGCTCAGTCTAGTTCCAGCACCTTTTCTTTTGGCTCAGGTGCAAATTTGGGTTTAAGTGCCATGACTTCTACCTCTGGCTTTGCTAGCACCCCTACTTCAGTCTTTGGTAGCGGGACTTCCTCAGTTCATAGCAATAGTACTTCCTTTTGTTTAAACAGTGTTACTCC
ACTTAAATCAAGTTCTGCGAGTTTTGGGACATCTTCTCTATTTGGAATTTCATCTAATGGTGCAAGTAATCAAACAAGTGGTATTAAGGAGCCTGTGGAAGGCAGTGCGGCAAAATTCATCACAGAGACAATAAGCTCAGTTAAATGTAGTCTTGGTGATACATCTTTGCCTTCAACTGAATTTGCTAGTTCATGTGGGA
CACAGAAAACAATGTCTCTATCAACAAACACAACTTCAGCGTCAGGAACCATGCCCATAGTAACTCCACTAGTTGATGCAACAAATCATTGCCAGACGACTGGAAAAGATATAGTAGAAGATGCTGCAACGCTATCCAGTCCTGTACCATCAGTCTTTGTTCAACAGCCTTCATTGATGGCCTCACCAACCTCAGTCACT
CTTGCAGTTGATTCTCAGTCAAATACACAATCAACTGAGGCTCAACCTGGCTCTCCAAGCCAAAATTTATCTGTTTCTGGTGCTCAAACTGCAATTGCATCGGCCGGTCATAATGAATCTACAGAGGGATCCTTTCCAGGTATCCGCAGTGAAAAAAATAGTCGGAAAGTCACAAGAGGTAAGCGGGATAAAACACGGAG
GAAGTAGAATGTAGCAGCTTGTAGGCACCGTTCAATTGTTGGTCGATCTTAACCAGACTTTCAGAAGACAAAAAGAGCCCTATTATTCCTTTTTTTTCAAGGAGGGGACTTGTCATTTTAATTATTTTTCAGGTAGATCTCAAGAGTCTAGCTGATTTTGTACAATTACTGCAATTTTGAATTGCTGTAAAAGATTTTTA
ATGTGCTTGTGGAGTGTAAGATATGCAGAACATGCGTCTCTGGGTATGCATGTAATTCATGTCTGTATCATAGTTTGTTGCTTCATAATGTATTTCCAAACTACTTGATGTGCCTGAATCCGATAGAAAGGTCTGACCAGCAGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCCTAAACTTAGACCTGGT
AAACAGTTGTTTATTTTAGTGGCAGCTTTGCATTTATCATGGAAACTCCTTAGGCGCTTGATATGCATTATATCCATGCGCACCCTTTCTGGAAATCCAGCAAAATAAATCCGATATGTTTAAAGTCACGGATAGATTCGGAGACGACGCCTAAAAACCTAAGGCAGTCGGGAACGGATAGATTCGGAGACGACGCCTAA
AAACCTAAGGCAGTCGGGAACTGTAAGCAAATCGTGTTTCTGGTGTGCAACGGAGCAGGAAATGTGCGCAAATTCCTTACTCTACCCTTTTCTCGCACAAGGACAGGGAAAAAAAATTATTAATGGAACGGATTCGGAATAAATGTAAAAATGCTTATTTACTCTTTTGGAGCAAATCTCACTTTTTGGCTACTGCAAGG
ACAACTATGATCAATTGTTAAAAATCATTGTCGTTTTCTTCTTACAAACTAAGTTTGAGTAATAGTCTATGAATTGTTTGATGTACTATGCAAAAGGGAAACTGGATATTGGATAAACATGTGGGAAATAAAAAATTTACACGGCCATTTCTTTGGAGGTCAAAATTGGGAAAAATTGCACATCATCCGCAATGTTAAAC
TGTGGAGAAGTATTAAACCTCCAAAAGCAGGGC