sp|Q9XH43|CET2_TOBAC ----> CEN-like protein 2

Intron 1 GTAACTAGTT ... AAATAACTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.17	155.1	51	5
A2	26.67	181.7	64.7	8.2
A3	22.07	195.3	54.3	2.8
A4	27.11	140.8	51.7	6
A5	27.31	114.6	42.3	0.2
A6	19.81	1590.8	387	24.8
A7	20.56	2181.8	553.7	25.7
A8	22.04	1930.6	536	18.3
A9	20.76	2096.7	540.7	23.7
A10	21.99	1930.1	535	23.3

>TRINITY_DN85341_c0_g1_i3 from sample A1 / Example spliced isoform

TTTTCATTCTTTCTGAAGGGCCATACACTATTCGCTTTGCTTTGGGCTTCTCCTGTATTTAGCAAAGATTGGACGACGCAAAGGTTGCAAATGGAGGGTTTAAGACAGCAGCTCATAGAATCTGAAGTTATAGGAGGCGTCGTCAACGATTTCTTTCCTCGTTTGAAGCTGTATGTATTCTTCGGCGAGAAACGAATTAG
CCTGGGTGATCATTTATTGCCTTCTGACGTCCAACACAAACCTCGCGCCTTCCAAATCGAATATGAAAACCTCGAGACAGAGGATGTCTGGACCAACTCCGGATTCGTATTGGTTATGACTGAACATCGCCGTCACTGCGAGTATTTGCACTGGTTGGTTACCGATATCATCCTCCAAGAACCTGCCACAGGTGGTGGTG
CCGGCGAAATTCAAGATCTAGTTTTCGGAGAGAAGGCTCTGGCTACCTATATACCTCCAAGATCCATGCTACCCCACAAGATAGTTTTCACATTGTTCAAGAAGAATGAATCTCAGCAAAGTATAACTCAATCCTTCTCTCGCGACATCTTCAGTACGAGAGCTTTTGCTAGTGTTAATGGTCTTGGACAGCCGCTCGGT
GCTGCTTTTTGCTACGTCCGTAATGTTATAACCAGAACTTTGACAGTGTCGCACTTCACGGATATATAGGTCAATGAGTAAATCGATCCACAAGCTTCACAATTTCAAGAAACGTTGAATATTCCGTAAATTTCCATGTACGTTGACGCGCAGTTTGAATATATTCAATAATCCAATTCCCGTGTCTCTTGTACAATATA
CAAAATATATATATTATACAATATATAAAGATCCTTTGCCCTTATGTCTTATATACATACATTAATTTTTCTTAC

>TRINITY_DN85341_c0_g1_i2 from sample A1 / Example unspliced isoform:

TTTTCATTCTTTCTGAAGGGCCATACACTATTCGCTTTGCTTTGGGCTTCTCCTGTATTTAGCAAAGATTGGACGACGCAAAGGTTGCAAATGGAGGGTTTAAGACAGCAGCTCATAGAATCTGAAGTTATAGGAGGCGTCGTCAACGATTTCTTTCCTCGTTTGAAGCTGTATGTATTCTTCGGCGAGAAACGAATTAG
CCTGGGTGATCATTTATTGCCTTCTGACGTCCAACACAAACCTCGCGCCTTCCAAATCGAATATGAAAACCTCGAGACAGAGGATGTCTGGATCAACTCCGGATTCGTATTGgtaactagttttaattccgatttcgaaattacagtcggcaataattatttagggtttgggttttggttataatttctgatattgaaat
tcatatttcgggtttgggttttggttataattttttatattgaaattcatattgataagtatcggtacatccaaataactagGTTATGACTGAACATCGCCGTCCCTGCGAGTATTTGCACTGGTTGG

Intron 2 GTATGCTACA ... TTCCGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.56	97.3	4	14.7
A2	20.58	107.1	5.9	17.7
A3	25.78	113.9	6.3	16.3
A4	30.72	101.3	4.5	21.7
A5	35.14	59.1	3.9	8.7
A6	26.34	1046	59.7	147.4
A7	28.04	1397.7	73.7	185.3
A8	33.38	1312.6	66.3	232.3
A9	30.53	1422.5	87.1	223.7
A10	25.25	1240.8	64.9	173.1

>TRINITY_DN119047_c0_g1_i2 from sample A2 / Example spliced isoform

TTCTTTCTGAAGGGCCATACACTATTCGCTTTGCTTTGGGCTTCTCCTGTATTTAGCAAAGATTGGACGACGCAAAGGTTGCAAATGGAGGGTTTAAGACAGCAGCTCATAGAATCTGAAGTTATAGGAGGCGTCGTCAACGATTTCTTTCCTCGTTTGAAGCTGTATGTATTCTTCGGCGAGAAACGAATTAGCCTGGG
TGATCATTTATTGCCTTCTGACGTCCAACACAAACCTCGCGCCTTCCAAATCGAATATGAAAACCTCGAGACAGAGGATGTCTGGACCAACTCCGGATTCGTATTGGTTATGACTGAACATCGCCGTCACTGCGAGTATTTGCACTGGTTGGTTACCGATATCATCCTCCAAGAACCTGCCACAGGTGGTGGTGCCGGCG
AAATTCAAGATCTAGTTTTCGGAGAGAAGGCTCTGGCTACCTATATACCTCCAAGATCCATGCTACCCCACAAGATAGTTTTCACATTGTTCAAGAAGAATGAATCTCAGCAAAGTATAACTCAATCCTTCTCTCGCGACATCTTCAGTACGAGAGCTTTTGCTAGTGTTAATGGTCTTGGACAGCCGCTCGGTGCTGCT
TTTTGCTACGTCCGTAATGTTATAACCAGAACTTTGACAGTGTCGCACTTCACGGATATATAGGTCAATGAGTAAATCGATCCACAAGCTTCACAATTTCAAGAAACGTTGAATATTCCGTAAATTTCCATGTACGTTGACGCGCAGTTTGAATATATTCAATAATCCAATTCCCGTGTCTCTTGTACAATATACAAAAT
ATATATATTATACAATATATAAAGATCCTTTGCCC

>TRINITY_DN119047_c0_g1_i1 from sample A2 / Example unspliced isoform:

ATTACAGTCGGCAATAATTATTTAGGGTTTGGGTTTTGGTTATAATTTCTGATATTGAAATTCATATTTCGGGTTTGGGTTTTGGTTATAATTTTTTATATTGAAATTCATATTGATAAGTATCGGTACATCCAAATAACTAGGTTATGACTGAACATCGCCGTCACTGCGAGTATTTGCACTGgtatgctacaatcttg
ctggatcatatcgatttctcattataatagcggtggactcctattcatctattagcaacattaattgattttccgtacagGTTGGTTACCGATATCATCCTCCAAGAACCTGCCACAGGTGGTGGTGCCGGCGAAATTCAAGATCTAGGTATTATGATATTTTTTTTTTATTCTGTGATCTTGAAATGGCTGTTATTGTT
TACTGTAATTATAAATATTGATCTGTTTCTTCGTTCAATATTAGTTTTCGGAGAGAAGGCTCTGGCTACCTATATACCTCCAAGATCCATGCTACCCCACAAGATAGTTTTCACATTGTTCAAGAAGAATGAATCTCAGCAAAGTATAACTCAATCCTTCTCTCGCGACATCTTCAGTACGAGAGCTTTTGCTAGTGTTA
ATGGTCTTGGACAGCCGCTCGGTGCTGCTTTTTGCTACGTCCGTAATGTTATAACCAGAACTTTGACAGTGTCGCACTTCACGGATATATAGGTCAATGAGTAAATCGATCCACAAGCTTCACAATTTCAAGAAACGTTGAATATTCCGTAAATTTCCATGTACGTTGACGCGCAGTTTGAATATATTCAATAATCCAAT
TCCCGTGTCTCTTGTACAATATACAAAATATATATATTATACAATATATAAAGATCCTTTGCCC

Intron 3 GTATTATGAT ... TCAATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.11	117.7	0.8	48
A2	20.07	117	2.3	29
A3	27.51	159.3	2	60
A4	13.84	113.3	1	18
A5	28.1	68	3.5	26
A6	24.92	1261.3	18.3	416
A7	25.88	1632.3	27.8	564.8
A8	24.22	1855.3	23.3	589.7
A9	21.98	1712.3	20.9	478.7
A10	23.93	1403.3	11.8	437.5

>TRINITY_DN119047_c0_g1_i2 from sample A2 / Example spliced isoform

TTCTTTCTGAAGGGCCATACACTATTCGCTTTGCTTTGGGCTTCTCCTGTATTTAGCAAAGATTGGACGACGCAAAGGTTGCAAATGGAGGGTTTAAGACAGCAGCTCATAGAATCTGAAGTTATAGGAGGCGTCGTCAACGATTTCTTTCCTCGTTTGAAGCTGTATGTATTCTTCGGCGAGAAACGAATTAGCCTGGG
TGATCATTTATTGCCTTCTGACGTCCAACACAAACCTCGCGCCTTCCAAATCGAATATGAAAACCTCGAGACAGAGGATGTCTGGACCAACTCCGGATTCGTATTGGTTATGACTGAACATCGCCGTCACTGCGAGTATTTGCACTGGTTGGTTACCGATATCATCCTCCAAGAACCTGCCACAGGTGGTGGTGCCGGCG
AAATTCAAGATCTAGTTTTCGGAGAGAAGGCTCTGGCTACCTATATACCTCCAAGATCCATGCTACCCCACAAGATAGTTTTCACATTGTTCAAGAAGAATGAATCTCAGCAAAGTATAACTCAATCCTTCTCTCGCGACATCTTCAGTACGAGAGCTTTTGCTAGTGTTAATGGTCTTGGACAGCCGCTCGGTGCTGCT
TTTTGCTACGTCCGTAATGTTATAACCAGAACTTTGACAGTGTCGCACTTCACGGATATATAGGTCAATGAGTAAATCGATCCACAAGCTTCACAATTTCAAGAAACGTTGAATATTCCGTAAATTTCCATGTACGTTGACGCGCAGTTTGAATATATTCAATAATCCAATTCCCGTGTCTCTTGTACAATATACAAAAT
ATATATATTATACAATATATAAAGATCCTTTGCCC

>TRINITY_DN119047_c0_g1_i1 from sample A2 / Example unspliced isoform:

ATTACAGTCGGCAATAATTATTTAGGGTTTGGGTTTTGGTTATAATTTCTGATATTGAAATTCATATTTCGGGTTTGGGTTTTGGTTATAATTTTTTATATTGAAATTCATATTGATAAGTATCGGTACATCCAAATAACTAGGTTATGACTGAACATCGCCGTCACTGCGAGTATTTGCACTGGTATGCTACAATCTTG
CTGGATCATATCGATTTCTCATTATAATAGCGGTGGACTCCTATTCATCTATTAGCAACATTAATTGATTTTCCGTACAGGTTGGTTACCGATATCATCCTCCAAGAACCTGCCACAGGTGGTGGTGCCGGCGAAATTCAAGATCTAGgtattatgatattttttttttattctgtgatcttgaaatggctgttattgtt
tactgtaattataaatattgatctgtttcttcgttcaatattagTTTTCGGAGAGAAGGCTCTGGCTACCTATATACCTCCAAGATCCATGCTACCCCACAAGATAGTTTTCACATTGTTCAAGAAGAATGAATCTCAGCAAAGTATAACTCAATCCTTCTCTCGCGACATCTTCAGTACGAGAGCTTTTGCTAGTGTTA
ATGGTCTTGGACAGCCGCTCGGTGCTGCTTTTTGCTACGTCCGTAATGTTATAACCAGAACTTTGACAGTGTCGCACTTCACGGATATATAGGTCAATGAGTAAATCGATCCACAAGCTTCACAATTTCAAGAAACGTTGAATATTCCGTAAATTTCCATGTACGTTGACGCGCAGTTTGAATATATTCAATAATCCAAT
TCCCGTGTCTCTTGTACAATATACAAAATATATATATTATACAATATATAAAGATCCTTTGCCC