NA ----> NA

Intron 1 GCAAGTAAGT ... GATTCAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.21	26	8.9	7
A2	22.64	41	3.1	12
A3	34.62	34	3.1	18
A4	15.63	27	5.9	5
A5	11.11	8	3.1	1
A6	18.82	69	13.8	16
A7	18.86	142	28.7	33
A8	20	132	19.2	33
A9	23.85	137.3	23.5	43
A10	25	90	11	30

>TRINITY_DN44754_c0_g2_i1 from sample A6 / Example spliced isoform

GTAATTGATATTTACTCGATTCCTGATTTGGCTCACCGATAAAAACAACTAAGAGTGACAAAATTTTGCATCATGGAAGGCCTGCGTAATTGATATTTACGAACGGCGGCCCAAATCCAATTCATTGTTTCGCCGCAGCGGTTTGCTGGAGCTGAATGATCCAGCCGTAAAAACTTAAAGAGGAAACAATGGAGAATCGG
TACATTACAACAACGGGGCCTGACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGGTAAGTTACCAATCTTCCTTTGTCCCCGCATTTAGTCTCCATATCTGTTAGCTCAAAGCTACTGTAAGCTCAAGCTAAACAGGTGACCTATTTTACATTTGATACGTTTCACGGGTGGGTCTCAG
AGGGGACTAATTTCCAAGCAATCTGTTATTTTAAGCGGAGGATTGAAATGGGTTATAGGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGCGGGGTCACCTATGTTGAAGACCCCGATGTCCCCCTCAACGTTCCCTAAAATTTGGGG
GATACTTCTACATAGGCCGCTCCCGGGTCCCCGCATTTCGGGAACTTGGTGGAACATTGCAAACAATCCACTAGGAGCAAGCAAAACTGTTATGAAGGAGAATGTTGCGTTTGGATGAAACTCTGACTCTTTTAGAGGCTAAGCTGAGGAGTGTAGATGGTTTTGAAGGAGTTGTAGGTCCTTCCTCCCTGCATGTACCA
CCAGATTTTCCATCTTCTGACAGTGTACCACAGGCAGCAAATTGTTCTGAAGATCCCTCAAGTGAAGCCATTCGGAATCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAATCCTGAGCAAAATTTGCAAATGGAAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCT
TCGGGTTGGCGTTCCAGAACAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTTGCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTATACGGTGCTATATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCAT
TAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCATGCAAACGGAATTCAGTCACTCAAACATTGGGTTGTAATGGAGCAGGAGAATAGTTTGCAATGAATAAAACTCATTTGCCACTCCAAGCATCTTAAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCA
AGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATAGTAGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATAATTTCTATACTAGGTCCATTTCTTGTGGCTCTCTAGTTTGAAGATGAAGCTGCTATGCCTTACTTGTTCCTCGGCAGTTTGTTTCCCGGAAGC
ATTTACTGAAAATATCGATTGATAATATCTTTGACCCTTATGTTTACAAGGATACAGATACATATGCATATATGCATATGTATCTGTATCCTTATGTTTACAAGGATACAGATACATATGCATATATGGGGATGGATATGTAAGCTTTTTAGAAAAGTTTGGATACGACATGGCTGAATATTAATCAGATTTTGTTCACT
CTGCAAGAGCATACATTCTTAGGGATGCCTTTTGATCTTAATTGTTTATAATATTCAAACAAGAGCTGCATGCAACCC

>TRINITY_DN44754_c0_g2_i10 from sample A6 / Example unspliced isoform:

CTAAAGCGGTGGTTCTTTAAATCACTGGTGTGTTCAGACTCTTAGAGCACAACCTTCAATTGGTTCAATTGCAACCGTATCCCATCTCCAGTCTCCAGGCAACTGTCTTCTGAAATTAAGACCCTGTAAAACAGATTTGACAGCCGGGTTTGGATTATGGCCTCCCATTATTCGATGGTTATCATATGTAGTCTCTTAAG
TGTACAAATCACGATCGCCTTTCCACTGTGTCTCACACAGGACGAAAAAGATCCCTGATATATTGAATTCTTGGACTTTCTTAACAATTATACAGCTACCATCAAACTATATCAACATCAGGGCCAGGTAGATTTATAATTGTGAGCTTGGTATCGGCAATCAACAACGCAGAAGGAAAGGGATGACCCAAGATTTTCTC
GATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAgcaagtaagtgccacatatttgtgatggcctgattttttatttcaaacaagtggattataagagtaaagtatacatgtcgaacatgtctgattatccaatatgcctagaaactctgcctccttgtccactagtgttgaacatttggctctatgttagaatttctat
ccatttttattgtcactgatgtgaaggcagtggcactgagttcaaaaagcatctctagagactctattatatggattcaacagACAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTTGCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTT
ATACGGTGCTATATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCATGCAAACGGAATTCAGTCACTCAAACATTG

Intron 2 GCGGGGTCAC ... TGTTATGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.6	26.2	11.4	14
A2	30.39	41	16.7	19
A3	21.14	43.2	13.9	9
A4	24.04	31.2	10.6	9
A5	30.18	13.4	6.6	5
A6	23.67	78	27.1	21
A7	23.61	119.8	41.6	32
A8	25.41	98.5	36.5	29.9
A9	29.35	115.3	60.6	33.9
A10	23.64	79.9	24.2	25

>TRINITY_DN121807_c12_g2_i13 from sample A2 / Example spliced isoform

GAGTAATTGATATTTACTCGATTCCTGATTTGGCTCACAGATAAAAACAACTAAGAGTGACAAAATTTTGCATCATGGAAGGCCTGCGTAATTGATATTTACGAACGGCGGCCCAAATCCAATTCATTGTTTCGCCGCAGCGGTTTGCTGGAGCTGAATGATCCAGCCGTAAAAACTTAAAGAGGAAACAATGGAGAATC
GGTACATTACAACAACGGGGCCTGACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGAGAATGTTGCGTTTGGATGAAACTCTGACTCTTTTAGAG
GCTAAGCTGAGGAGTGTAGATGGTTGTGAAGGAGTTGTAGGTCCTTCCTCCCTGCATGTACCACCAGATTTTCCATCTTCTGACAGTGTACCACAGGCAGCAAATTGTTCTGAAGATCCCTCAAGTGAAGCCATTCGGACTCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAA
TCCTGAGCAAAATTTGCAAATGGAAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAGCAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTTGCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTAT
ACGGTGCTATATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCATGCAAACGGAATTCAGTCACTCAAACATTGCAGGAGAATTCAGTCACTCAAACGGAATTGCAGGAGAATAG
TTTGCAATGAATAAAACTCATTTGCCACTCCAAGCATCTTAAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCAAGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATAGTAGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATTATTTCTATACTAG
GTCCATTTCTTGTGGCTCTGCAGTTTGAAGATGAAGCTGCTATGCCTTATTTGTTCCTCGGCAGTTTGTTTCCCGGAAGCATTTACTGAAAATATCTATTGATAATATCTTTGACCCTTATGTTTACAAGGATACAGATACATATGCATATATGGGGATGGATATGTAAGCTTTTTAGAAAAGTTTGGATACGACATGGC
TGAATATTAATCAGATTTTGTTCACTCA

>TRINITY_DN121807_c12_g2_i9 from sample A2 / Example unspliced isoform:

TTTGCTGGAGCTGAATGATCCAGCCGTAAAAACTTAAAGAGGAAACAATGGAGAATCGGTACATTACAACAACGGGGCCTGACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGAGGGGACTAATTTCCAAGCAATCTGTTATTTTAAGCGGAGGATTGAAATGGGTTATAGGTGACAGT
CTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGgcggggtcacctatgttgaagaccccgatgtccccctcaacgttccctaaaatttgggggatacttctacataggccgctcccgggtccccgcatttcgggaacttggtggaacattgcaaacaa
tccactaggagcaagcaaaactgttatgaagGAGAATGTTGCGTTTGGATGAAACTCTGACTCTTTTAGAGGCTAAGCTGAGGAGTGTAGATGGTTGTGAAGGAGTTGTAGGTCCTTCCTCCCTGCATGTACCACCAGATTTTCCATCTTCTGACAGTGTACCACAGGCAGCAAATTGTTCTGAAGATCCCTCAAGTGAA
GCCATTCGGACTCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAATCCTGAGCAAAATTTGCAAATGGAAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAGCAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTT
GCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTATACGGTGCTATATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCA
TGCAAACGGAATTCAGTCACTCAAACATTGCAGGAGAATTCAGTCACTCAAACGGAATTGCAGGAGAATAGTTTGCAATGAATAAAACTCATTTGCCACTCCAAGCATCTTAAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCAAGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATAGT
AGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATTATTTCTATACTAGGTCCATTTCTTGTGGCTCTGCAGTTTGAAGATGAAGCTGCTATGCCTTATTTGTTCCTCGGCAGTTTGTTTCCCGGAAGCATTTACTGAAAATATCTATTGATAATATCTTTGACCCTTATGTTTACAA
GGATACAGATACATATGCATATATGGGGATGGATATGTAAGCTTTTTAGAAAAGTTTGGATACGACATGGCTGAATATTAATCAGATTTTGTTCACTCA

Intron 3 GTACGTTACC ... TTATTTTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.21	19	12.2	12
A2	38.6	22.8	6	14
A3	25.65	31.8	20.7	10
A4	46.28	15.2	8.3	12.3
A5	63.77	6.2	7.5	9.8
A6	38.51	35.8	12.3	19.8
A7	13.6	71	19	10.2
A8	23.17	42.7	14.2	11
A9	24.18	68.8	25.3	19.7
A10	20.56	41.5	7.5	9.8

>TRINITY_DN121807_c12_g2_i12 from sample A2 / Example spliced isoform

GAGTAATTGATATTTACTCGATTCCTGATTTGGCTCACAGATAAAAACAACTAAGAGTGACAAAATTTTGCATCATGGAAGGCCTGCGTAATTGATATTTACGAACGGCGGCCCAAATCCAATTCATTGTTTCGCCGCAGCGGCTTGCTGGAGCGGACGTAAAAACTTAAAGAGGAAACGATGGAGAATCGGTACAATAC
AACAACGGGGCCTGACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGCGGAGGATTGAAATGGGTTATAGGTGACCGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCG

>TRINITY_DN121807_c12_g2_i1 from sample A2 / Example unspliced isoform:

GAGAAGAGGACAATTTACCTGgtaagttaccaatcttcctttgtccccgcatttagtctccatatctgttagctcaaagctactgtaagctcaagctaaacaggtgacctattttacatttgatacgtttcacgggtgggtctcagaggggactaatttccaagcaatctgttattttaagCGGAGGATTGAAATGGGTT
ATAGGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGCGGGGTCACCTATGTTGAAGACCCCGATGTCCCCCTCAACGTTCCCTAAAATTTGGGGGATACTTCTACATAGGCCGCTCCCGGGTCCCCGCATTTCGGGAACTTGGTGGAA
CATTGCAAACAATCCACTAGGAGCAAGCAAAACTGTTATGAAGGTGTGTACCCCTAATGTAAATTTTCCAATGCGTAGGTGATTGGAGTCCTTATGTTCTCAACCAAACAAATCTGTTATTTAATTGCAGAAA

Intron 4 GTAAGTTACC ... TGGGTCTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.38	12.2	11.6	7
A2	40.74	9.8	5.7	8
A3	51	16	19.9	14
A4	33.85	18	7.8	11
A5	65.61	4	7.3	8
A6	41.64	15	11.8	10
A7	21.57	42.3	18	6
A8	27.32	26.8	13.6	7
A9	40.28	32.8	24.1	21
A10	14.29	20	7	0

>TRINITY_DN121807_c12_g2_i9 from sample A2 / Example spliced isoform

TTTGCTGGAGCTGAATGATCCAGCCGTAAAAACTTAAAGAGGAAACAATGGAGAATCGGTACATTACAACAACGGGGCCTGACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGAGGGGACTAATTTCCAAGCAATCTGTTATTTTAAGCGGAGGATTGAAATGGGTTATAGGTGACAGT
CTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGCGGGGTCACCTATGTTGAAGACCCCGATGTCCCCCTCAACGTTCCCTAAAATTTGGGGGATACTTCTACATAGGCCGCTCCCGGGTCCCCGCATTTCGGGAACTTGGTGGAACATTGCAAACAA
TCCACTAGGAGCAAGCAAAACTGTTATGAAGGAGAATGTTGCGTTTGGATGAAACTCTGACTCTTTTAGAGGCTAAGCTGAGGAGTGTAGATGGTTGTGAAGGAGTTGTAGGTCCTTCCTCCCTGCATGTACCACCAGATTTTCCATCTTCTGACAGTGTACCACAGGCAGCAAATTGTTCTGAAGATCCCTCAAGTGAA
GCCATTCGGACTCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAATCCTGAGCAAAATTTGCAAATGGAAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAGCAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTT
GCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTATACGGTGCTATATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCA
TGCAAACGGAATTCAGTCACTCAAACATTGCAGGAGAATTCAGTCACTCAAACGGAATTGCAGGAGAATAGTTTGCAATGAATAAAACTCATTTGCCACTCCAAGCATCTTAAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCAAGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATAGT
AGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATTATTTCTATACTAGGTCCATTTCTTGTGGCTCTGCAGTTTGAAGATGAAGCTGCTATGCCTTATTTGTTCCTCGGCAGTTTGTTTCCCGGAAGCATTTACTGAAAATATCTATTGATAATATCTTTGACCCTTATGTTTACAA
GGATACAGATACATATGCATATATGGGGATGGATATGTAAGCTTTTTAGAAAAGTTTGGATACGACATGGCTGAATATTAATCAGATTTTGTTCACTCA

>TRINITY_DN121807_c12_g2_i1 from sample A2 / Example unspliced isoform:

GAGAAGAGGACAATTTACCTGgtaagttaccaatcttcctttgtccccgcatttagtctccatatctgttagctcaaagctactgtaagctcaagctaaacaggtgacctattttacatttgatacgtttcacgggtgggtctcagAGGGGACTAATTTCCAAGCAATCTGTTATTTTAAGCGGAGGATTGAAATGGGTT
ATAGGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGCGGGGTCACCTATGTTGAAGACCCCGATGTCCCCCTCAACGTTCCCTAAAATTTGGGGGATACTTCTACATAGGCCGCTCCCGGGTCCCCGCATTTCGGGAACTTGGTGGAA
CATTGCAAACAATCCACTAGGAGCAAGCAAAACTGTTATGAAGGTGTGTACCCCTAATGTAAATTTTCCAATGCGTAGGTGATTGGAGTCCTTATGTTCTCAACCAAACAAATCTGTTATTTAATTGCAGAAA

Intron 5 GTAAGTGCCA ... ATTGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.75	26	5.5	6
A2	38.81	41	1	26
A3	37.04	34	0	20
A4	20.59	27	1	7
A5	20	8	0	2
A6	19.32	71	3	17
A7	19.77	138	8	34
A8	20.61	131	1	34
A9	24.59	138	2	45
A10	25.21	89	2.5	30

>TRINITY_DN42877_c1_g2_i4 from sample A9 / Example spliced isoform

GCCCGGATGGCAGTCCTGAGTAATTGATATTTACTCGATTCCTGATTTGGCTCACCGATAAAAACAACTAAGAGTGACAAAATTTTGCATCATGGAAGGCCTGCGTAATTGATATTTACGAACGGCGGCCCAAATCCAATTCATTGTTTCGCCGCAGCGGTTTGCTGGAGCTGAATGATCCAGCCGTAAAAACTTAAAGA
GGAAACAATGGAGAATCGGTACATTACAACAACGGGGCCTGACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGGTAAGTTACCAATCTTCCTTTGTCCCCGCATTTAGTCTCCATATCTGTTAGCTCAAAGCTACTGTAAGCTCAAGCTAAACAGGTGACCTATTTTACATTTGATACG
TTTCACGGGTGGGTCTCAGAGGGGACTAATTTCCAAGCAATCTGTTATTTTAAGCGGAGGATTGAAATGGGTTATAGGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGCGGGGTCACCTATGTTGAAGACCCCGATGTCCCCCTCAA
CGTTCCCTAAAATTTGGGGGATACTTCTACATAGGCCGCTCCCGGGTCCCCGCATTTCGGGAACTTGGTGGAACATTGCAAACAATCCACTAGGAGCAAGCAAAACTGTTATGAAGTGAAGCCATTCGGAATCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAATCCTGAGCA
AAATTTGCAAATGGAAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAGCAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTTGCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTATACGGTGCTA
TATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCATGCAAACGGAATTCAGTCACTCAAACATTGGGTTGTAATGGAGCAGGAGAATAGTTTGCAATGAATAAAACTCATTTGCC
ACTCCAAGCATCTTAAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCAAGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATAGTAGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATAATTTCTATACTAGGTCCATTTCTTGTGGCTCTCTAGTTT
GAAGATGAAGCTGCTATGCCTTATTTGTTCCTCGGCAGTTTGTTTCCCGGAAGCATTTACTGAAAATATCGATTGATAATATCTTTGACCCTTATGTTTACAAGGATACAGATACATATGCATATATGGGGATGGATATGTAAGCTTTTTAGAAAAGTTTGGATACGACATGGCTGAATATTAATCAGATTTTGTTCACT
CTGCAAGAGCATACATTCTTAGGGATGCCTTTTGATCTTAATTGTTTATAATATTCAAACAAGAGCTGCATGCAACCC

>TRINITY_DN42877_c1_g2_i10 from sample A9 / Example unspliced isoform:

CTTTTACAGTGTAAATTATCATAGAAGTGAAAAGTAAGAAGATTATCACGTTTACTTTCTTGACAAATTTTCAAGGTGTCAGTTACTGTATGATTTCTATATCCAAAGCTTGCATTTAGAACTGTGTTTGGCACTCGTGTTTTTGTTATACTTGTTTTCCTGACACTCTCGATGAAGTAAAACTTCGGCATTTCCCACTT
CTTTTGCAAATATGTTGCATTGTGGGCCTAAGACTCTAAGAGTAAACTTGTGTTGCAGAGGAATCCTATTTCAATTCTCTCCTGCTGCCTGCCAGTGTAAATTAGTAATTTAGTATGCATGCATATTAACAAAAAAATGTCTTATGCCTCGACTTCATTCTATCTATGTTTTGCATTAATGATATATATTCTGTTATGAT
TAAAGCTAGTAGTGGATCAGTTCCAGTTTTCCTACATTTAAACTTTTATCTGTACATTTTTTAAGTTTACGTGACCACATTTTCTTTTTAACACTAACTTTTCTGTACAGGAAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAGCAAgtaagtgccacatatttgtgatggcct
gattttttatttcaaacaagtggattataagagtaaagtatacatgtcgaacatgtctgattatccaatatgcctagaaactctgcttccttgtccactagtgttgaacatttggctctatgttagaatttctatccatttttattgtcactgatgtgaaggcagtggcactgagttcaaaaagcatctctagagactct
attatatggattcaacagagtaatgcaaccaaaaactatttgttttataaccaatttggaagtggtgtgagatattttgctgatgcaatttgcatggtatggtttatgtgctttgatagatgtatgcaccatgaaagtatggattattgatccggttatgcttgaattgatgcagGCAGTAAAGCTACAGATGTCCATTG
AAGGACTAGATCCTAATTTGCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTATACGGTGCTATATTTTGTCAGCAATTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATT
CTCTTTCTTGGCCAGTGCATGCAAACGGAATTCAGTCACTCAAACATTGGGTTGTAATGGAGCAGGAGAATAGTTTGCAATGAATAAAACTCATTTGCCACTCCAAGCATCTTAAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCAAGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATA
GTAGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATAATTTCTATACTAGGTCCATTTCTTGTGGCTCTCTAGTTTGAAGATGAAGCTGCTATGCCTTATTTGTTCCTCGGCAGTTTGTTTCCCGGAAGCATTTACTGAAAATATCGATTGATAATATCTTTGACCCTTATGTTTAC
AAGGATACAGATACATATGCATATATGGGGATGGATATGTAAGCTTTTTAGAAAAGTTTGGATACGACATGGCTGAATATTAATCAGATTTTGTTCACTCTGCAAGAGCATACATTCTTAGGGATGCCTTTTGATCTTAATTGTTTATAATATTCAAACAAGAGCTGCATGCAACCC

Intron 6 GTACGTTACC ... TGGGTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.26	28	11.6	15.3
A2	31.8	36.1	5.9	16.9
A3	14.72	58.4	20	10.4
A4	35.73	32.1	7.4	17.9
A5	50.17	11.7	7.4	12
A6	26.9	75.7	11.4	28.1
A7	20.3	134.9	17.6	34.7
A8	23.65	100.4	13.4	31.4
A9	21.43	148.4	22.9	40.7
A10	17.09	79.9	6.9	16.7

>TRINITY_DN124219_c0_g1_i9 from sample A1 / Example spliced isoform

CCTGATTTGGCTCACAGATAAAAACAACTAAGAGTGACAAAATTTTGCATCATGGAAGGCCTGCGTAATTGATATTTACGAACGGCGGCCCAAATCCAATTCATTGTTTCGCCGCAGCGGTTTGCTGGAGCTGAATGATCCAGCCGTAAAAACTTAAAGAGGAAACAATGGAGAATCGGTACATTACAACAACGGGGCCT
GACGAGGAGGACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGAGAATGTTGCGTTTGGATGAAACTCTGACTCTTTTAGAGGCTAAGCTGAGGAGTGTAGATGG
TTTTGAAGGAGTTGTAGGTCCTTCCTCCCTGCATGTACCACCAGATTTTCCATCTTCTGACAGTGTACCACAGGCAGCAAATTGTTCTGAAGATCCCTCAAGTGAAGCCATTCGGAATCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAATCCTGAGCAAAATTTGCAAATGG
AAAGGGATGACCCAAGATTTTCTCGATTTTTACGAATGCTTCGGGTTGGCGTTCCAGAACAAGCAGTAAAGCTACAGATGTCCATTGAAGGACTAGATCCTAATTTGCTCAATCTATCCGATGCTATGCAGTCGCCTGGTTAAATATTAGTGTTTCAATAGGAAATTGATGGTTTATACGGTGCTATATTTTGTCAGCAA
TTAATTTATTTGCATTCATGTTTCTCAGCAAATTATGCATTAGAAAGGCTTTACATAGTCTTTTATCTCGTGAGAATTCAGATAATTCTCTTTCTTGGCCAGTGCATGCAAACGGAATTCAGTCACTCAAACATTGGGTTGTAATGGAGCAGGAGAATAGTTTGCAATGAATAAAACTCATTTGCCACTCCAAGCATCTT
AAAACAAGAGATCATTTCCAAATATGTATGCGAACAACCAAGAATGACTACTGCCAAACAATGATCCTTTTTGTTCTGTTAATAATAGTAGGCACTAGGCAGGCATTATATGTACGGCTTTAAACAAAACCAGCATTGTATTAAATTATTTCTATACTAGGTCCATTTCTTGTGGCTCTGCAGTTTGAAGATGAAGCTGC
TATGCCTTACTTGTTCCTCGGCAGTTTGTTTCCCGGAAGCATTTACTGAAAATATCTATTGATAATATCTTTGACCCTTATGTTTACAAGGATACAGATACATATGCATATATGGGGATGGATACGTAAGCTTTTTAGAAAATTTTGGATACGACATGGCTGAATATTAATCAGATTTTGTTCACTCTGCAAGAGCATAC
ATTCTTAGGGATGCCTTTTGATCTTAATTTTTATAATATTCAAACAAGAGCTGC

>TRINITY_DN124219_c0_g1_i8 from sample A1 / Example unspliced isoform:

CCTGATTTGGCTCACAGATAAAAACAACTAAGAGTGACAAAATTTTGCATCATGGAAGGCCTGCGTAATTGATATTTACGAACGGCGGCCCAAATCCAATTCATTGTTTCGCCGCAGCGGCTTGCTGGAGCGGACGTAAAAACTTAAAGAGGAAACGATGGAGAATCGGTACAATACAACAACGGGGCCTGACGAGGAGG
ACGAATCATGGATACCCATGTCTGAGAAGAGGACAATTTACCTGgtaagttaccaatcttcctttgtccccgcatttagtctccatatctgttagctcaaagctactgtaagctcaagctaaacaggtgacctattttacatttgatacgtttcacgggtgggtctcagaggggactaatttccaagcaatctgttattt
taagcggaggattgaaatgggttatagGTGACAGTCTTCATCGTCCACACTACAAGGTTTTTGAACAGATTCTCGGCATTATGCGAGCAGAAACTGGCAGAAGTACACCGGAGAATGTTGCGTTTGGATGAAACTCTGACTCTTTTAGAGGCTAAGCTGAGGAGTGTAGATGGTTTTGAAGGAGTTGTAGGTCCTTCCTC
CCTGCATGTACCACCAGATTTTCCATCTTCTGACAGTGTACCACAGGCAGCAAATTGTTCTGAAGATCCCTCAAGTGAAGCCATTCGGAATCCACCAGAAAGCTGTAAAACCTCAGAGCAAGAAAAAAACAAAATAATTTCACCTCCAAATCCTGAGCAAAATTTGCAAATGGTCAGTTGAATACTCCTTTATATTTTGA
TTTCTTGTTTGCACCTGATCTTTTATGCATTCTCTTTCCATGTTCGCTTATACGATATATGACTGCTATTCTGTTTTATGGGTTGTTATTGCAATTCTTTAAGTGTTTATGTAATTTGATCTTAAAAACTGGGTTGCAGCAAATGTCAATATTTTTTCCTTGTGATATATACGTAATGTCAGAAAAACCACACAAGTACG
CAGACAATGAATGCAAGCCAATATAAGAGGGACCTTCCCAGACTCAAGGAGTTTGTACAGTGAGACATCAGTGTTCTAGCAGTAGGAGACTGTTTAGAAGAATCCCAGGCTGTGCATACAAGGGGAATCTTGTCAATGCTCGAAACCGAATTAAACGACAAGCTCACCAACACAGGCCAGAGTGTGAATTTGAAGGAGAT
CGAGTGTTTGTATATCTGCAACCCTATAAGCAACTGTCACTTGGATAAAATGGAAAAACAAGCTAACACCCAATTTCTACAGGCTTATCAAATCAAGAGAAACATAAGTAGTGTTTCTTATGCCTTGGATCTAACCTAGCACAAGCTGTATTCATAATGTATTCAGTGTATCATGTTTAAAGAAGAATTTGGGGAGTCAT
GATGAACCACAAACAGAACTACTTATGCTTGATGAAGAGGGAGTGAAAACTTATTTTAGAACCCCAAGGTGTAACAGACATTCAGAGAAAACCCCGTCTATCCAGAACAATGATCAAATACCTCATCAAATGGAAAAGTTTACTGAGTGAAGGACCTTCTGGGAATTGAAGCCTTCCGACAACATCTGTCCTTGCCAAAG
ATTTTAGGACACAGCTCTCTGAAGGGGGAGGACATGTAATGAACATAAATATTAGGTTATATTAATAATTAATTATAATTATATGGCTGG