NA ----> NA

Intron 1 GTTTGATTTT ... GTTATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.79	268	58	35
A2	21.71	202	56	22.5
A3	18.42	217	49	15.5
A4	20.66	192	50	23.5
A5	13.73	88	14	7.5
A6	20.2	486	123	51
A7	21.57	967	266	66.5
A8	18.78	1328	307	138.5
A9	19.67	880	215.5	75.5
A10	19.63	700	171	33.5

>TRINITY_DN128981_c2_g2_i6 from sample A1 / Example spliced isoform

CTGCAAAGCCTCCAATGGCAACTGAAGGTGGGAGACGGCGTCAAAGACTTATTCTTCAGGAAAATTCAGATGATGAAGATGACTTTCCTGTAAGGGTAGAAATGCAAGAATCACCTAAGGAGCCTCAATTTAATACCAGTGCATCTATTGCAAAGATGGATACTCATCATGCCAAGGAAATTACCTCTGGAAAGCAGTCA
GAAATGTCTAACAATGCTGTAGCTGGCCAACCTGTTCGCAAAAGCTCAAGGGTACCTAAGAAACGCATACTAGACGGTGAACATGATGAGGATGATGATGGTGAGGAAGCATCACAGCGTAAAAGCTCAAGGAACCCCAAGAAACGTGTTTCAATTGGTGAGTATGATGAAGATGAGGAGGGACATGAAACACCTCAGCG
GCGAAGTTCAAGGATGTCTAAGAAGCGCATGCTGGATGCTGAATATGATGAATATGATGAGGAAGATGAAGTGCAGCAACATAAAAGAAGACTGAAAAGGCCAAAATATGAACGGGATTCAGAAGATTTATATCAGGAAGATGAAGAAGAAGAAGCTGCATCAGCTGATGAAGATGTGGAAATTGTGGATGTTGAACTTG
AAGGAAAGAAGAAGAGAAGCAGACGAAAGCAAGAAGTTGGAAATTCGACTTTTGATGATAAGAAGGAAGCTCCTCTTACTGCACGCCAAAGGGCTATGCAGTCATCCAGGGATGTAGATTCAGAAGTAGGAGCAAATCTGATTGAATTTCCAGAAGGATTGCCTCAGACCAGTCAGAGAAAACGGAAAGAAAAGTTATCG
GAGGTGGAACAGCAGTTGAAGAAAGCAGAGGCAGCTCAGAGACGCAAGATGCAAGTAGAGAAGGCTGCGAGTGAGATTCAGGCTGAGGCCATCAAGAAGATTCTGGGTCAAGACTCTTTGAGGAAGAAACGGGAAGATAAGCTACGGAAGCAAAGAGATGAGATGGCACAGGAGAAGGCTGCTAGTGCTATGATGCTTGC
ATCAAACAGTGTGAGATGGGTCATGGGACCTTCTGGCACAGTTGTTTCATTTTCTGAAAATACAGGGCTTCCGTGCATATTCAATTCTGGCCCATGCAGGTATCCTCCACCTCGTGAGAAATGTGCCGGGCCATCATGTAATAATACATATAAATATAGAGATTCTAAGTCTAAGCTCCCTCTATGCAGTCTTCAGTGCT
ACCGTGCTATACATGATGTTACACATCCTGTCTCTACTTGTTAGTGTTGCAGAATTTATTGCTGTTTTATGTCTCCTCTCAAGAGCACAGGCATACAAGCACAGTATATGTTCATATGTTACTTTTCTGAAGTTTGGAATAGATTTTAGTGCATTTCGCCTTAGTGTTTTTGATGTATATATGCATGAATCATTCGAGAT
GGAGGGGAGGTTCATACTGTATCTATCCAAGGAAGGCTTCATATTATGTATCATCATGACAAAGCTAATGTTTTGGAATAAACAGGAAGCTGAGTTGGGCATAATTGTATCTAAAGAATCATTTTTATGTATAGATAAATCAATTATACGGAAAAGATTGGAGATTAATATATCAAGATGTTAATTTTTGGAATTGATTA
ACCAGAGAAAATATGGAACAGTGGCGGTTTACTTGTAAATTGGATTCCTTCTGCTGCTACAATTAATTTTGCCATTTTATATGTTATGCAGCTCGATTCCTTGTAAATAGTGTG

>TRINITY_DN128981_c2_g2_i9 from sample A1 / Example unspliced isoform:

GCAAATCTGATTGAATTTCCAGAGGGATTGACTCAGACCAGTCAGGAAAAACGGAAAGAAAAGTTATCGGAGGTGGAACAGCAGTTGAAGAAAGCAGAGGCAGCTCAGAGACGCAAGATGCAAGTAGAGAAGGCTGCGAGTGAGATTCAGGTATGCATAGCTATCTGTGGTCGGGTTACATTTGTAGGATTTTCTAGTGG
GAATGTTTTTCATAGATAAAATTTATCTCAAATTTTTAACGTAACTAAGTGCTTATGTGATCACAGGCTGAGGCCATCAAGAAGATTCTGGGTCAAGACTCTTTGAGGAAGAAACGGGAAGATAAGCTACGGAAGCAAAGAGATGAGATGGCACAGGAGAAGGCTGCTAGTGCTATGATGCTTGCATCAAACAGTGTGAG
ATGGGTCATGGGACCTTCTGGCACAGTTGTTTCATTTTCTGAAAATACAGGGCTTCCGTGCATATTCAATTCTGGCCCATGCAGgtttgattttactttctaatttctttttatgatagtatttttagtcttcaatcagccttgaattagtattactaattggcagcaatcatttatttttcaagctacttccacagaag
atgattttcttttcccatcatttattgttattgtagGTATCCTCCACCTCGTGAGAA

Intron 2 GTGATTTCCA ... TTGACAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.67	382	82	6
A2	16.49	324	64	4
A3	16.3	359.5	70	1
A4	12.94	222	33	2
A5	9.17	109	11	0
A6	11.37	522.5	67	0
A7	12.21	1474	205	0
A8	12.53	1606	230	1
A9	11.3	1248	159	0
A10	12.96	1242	185	1

>TRINITY_DN43358_c1_g3_i2 from sample A8 / Example spliced isoform

TACTGCACGCCAAAGGGCTATGCAGTCATCCAGGGATGTAGATTCAGAAGTAGGAGCAAATCTGATTGAATTTCCAGAAGGATTGCCTCAGACCAGTCAGAGAAAACGGAAAGAAAAGTTATCGGAGGTGGAACAGCAGTTGAAGAAAGCAGAGGCAGCTCAGAGACGCAAGATGCAAGTAGAGAAGGCTGCGAGTGAGA
TTCAGGCTGAGGCCATCAAGAAGATTCTGGGTCAAGACTCTTTGAGGAAGAAACGGGAAGATAAGCTACGGAAGCAAAGAGATGAGATGGCACAGGAGAAGGCTGCTAGTGCTATGATGCTTGCATCAAACAGTGTGAGATGGGTCATGGGACCTTCTGGCACAGTTGTTTCATTTTCTGAAAATACAGGGCTTCCGTGC
ATATTCAATTCTGGCCCATGCAGGTTTGATTTTACTTTCTAATTTCTTTTTATGATAGTATTTTTAGTCTTCAATCAGCCTTGAATTAGTATTACTAATTGGCAGCAATCATTTATTTTTCAAGCTACTTCCACAGAAGATGATTTTCTTTTCCCATCATTTATTGTTATTGTAGGTATCCTCCACCTCGTGAGAAATGT
GCCGGGCCATCATGTAATAATACATATAAATATAGAGATTCTAAGTCTAAGCTCCCTCTATGCAGTCTTCAGTGCTACCGTGCTATACATGATGTTACACATCCTGTCTCTACTTGTTAGTGTTGCAGAATTTATTGCTGTTTTATGTCTCCTCTCAAGAGCACAGGCATACAAGCACAGTATATGTTCATATGTTACTT
TTCTGAAGTTTGGAATAGATTTTAGTGCATTTCGCCTTAGTGTTTTTGATGTATATATGCATGAATCATTCGAGATGGAGGGGAGGTTCATACTGTATCTATCCAAGGAAGGCTTCATATTATGTATCATCATGACAAAGCTAATGTTTTGGAATAAACAGGAAGCTGAGTTGGGCATAATTGTATCTAAAGAATCATTT
TTATGTATAGATAAATCAATTATACGGAAAAGATTGGAGATTAATATATCAAGATGTTAATTTTTGGAATTGATTAACCAGAGAAAATATGGAACAGTGGCGGTTTACTTGTAAATTGGATTCCTTCTGCTGCTACAATTAATTTTGCCATTTTATATGTTATGCAGCTCGATTCCTTGTAAATAGTGTGTGGTTAATGT
TTCTTACTATTGTACCGGGGAATACTCTGGTTGTAAATATCTCTTTTATATTTATTTCAATTTTTTGTATATTTTATGGGTACGTGGATTTTTTCGGGATCCAATGTAGGCCCTACTATTCTAGAATCATGGCAAAGATGTAAGATAACTATAGAAAAGTTGGCCATCGTATTATGTGGCAATGTCTTAATGAGTTCAAT
TGTGTCTTGAACATGACACCATGAACAACTGTAAC

>TRINITY_DN43358_c1_g3_i5 from sample A8 / Example unspliced isoform:

TACTGCACGCCAAAGGGCTATGCAGTCATCCAGGGATGTAGATTCAGAAGTAGGAGCAAATCTGATTGAATTTCCAGAAGGATTGCCTCAGACCAGTCAGAGAAAACGGAAAGAAAAGTTATCGGAGGTGGAACAGCAGTTGAAGAAAGCAGAGGCAGCTCAGAGACGCAAGATGCAAGTAGAGAAGGCTGCGAGTGAGA
TTCAGGCTGAGGCCATCAAGAAGATTCTGGGTCAAGACTCTTTGAGGAAGAAACGGGAAGATAAGCTACGGAAGCAAAGAGATGAGATGGCACAGgtgatttccagtcctgtggctgtgtatgagtgttgtctctttctctattgctttccccttaatcttttatttatagtattaagctatgttcttgacaacagGAGA
AGGCTGCTAGTGCA

Intron 3 GTATGCATAG ... GTGATCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.78	288	42	100
A2	17.38	259	36	73
A3	16.18	259	39	61
A4	17.38	164	32	37
A5	19.51	66	9	23
A6	16.32	259	28	73
A7	17.47	848	117	242
A8	15.6	990	136	230
A9	16.07	697	120	147
A10	14.4	755	97	157

>TRINITY_DN128981_c2_g2_i6 from sample A1 / Example spliced isoform

CTGCAAAGCCTCCAATGGCAACTGAAGGTGGGAGACGGCGTCAAAGACTTATTCTTCAGGAAAATTCAGATGATGAAGATGACTTTCCTGTAAGGGTAGAAATGCAAGAATCACCTAAGGAGCCTCAATTTAATACCAGTGCATCTATTGCAAAGATGGATACTCATCATGCCAAGGAAATTACCTCTGGAAAGCAGTCA
GAAATGTCTAACAATGCTGTAGCTGGCCAACCTGTTCGCAAAAGCTCAAGGGTACCTAAGAAACGCATACTAGACGGTGAACATGATGAGGATGATGATGGTGAGGAAGCATCACAGCGTAAAAGCTCAAGGAACCCCAAGAAACGTGTTTCAATTGGTGAGTATGATGAAGATGAGGAGGGACATGAAACACCTCAGCG
GCGAAGTTCAAGGATGTCTAAGAAGCGCATGCTGGATGCTGAATATGATGAATATGATGAGGAAGATGAAGTGCAGCAACATAAAAGAAGACTGAAAAGGCCAAAATATGAACGGGATTCAGAAGATTTATATCAGGAAGATGAAGAAGAAGAAGCTGCATCAGCTGATGAAGATGTGGAAATTGTGGATGTTGAACTTG
AAGGAAAGAAGAAGAGAAGCAGACGAAAGCAAGAAGTTGGAAATTCGACTTTTGATGATAAGAAGGAAGCTCCTCTTACTGCACGCCAAAGGGCTATGCAGTCATCCAGGGATGTAGATTCAGAAGTAGGAGCAAATCTGATTGAATTTCCAGAAGGATTGCCTCAGACCAGTCAGAGAAAACGGAAAGAAAAGTTATCG
GAGGTGGAACAGCAGTTGAAGAAAGCAGAGGCAGCTCAGAGACGCAAGATGCAAGTAGAGAAGGCTGCGAGTGAGATTCAGGCTGAGGCCATCAAGAAGATTCTGGGTCAAGACTCTTTGAGGAAGAAACGGGAAGATAAGCTACGGAAGCAAAGAGATGAGATGGCACAGGAGAAGGCTGCTAGTGCTATGATGCTTGC
ATCAAACAGTGTGAGATGGGTCATGGGACCTTCTGGCACAGTTGTTTCATTTTCTGAAAATACAGGGCTTCCGTGCATATTCAATTCTGGCCCATGCAGGTATCCTCCACCTCGTGAGAAATGTGCCGGGCCATCATGTAATAATACATATAAATATAGAGATTCTAAGTCTAAGCTCCCTCTATGCAGTCTTCAGTGCT
ACCGTGCTATACATGATGTTACACATCCTGTCTCTACTTGTTAGTGTTGCAGAATTTATTGCTGTTTTATGTCTCCTCTCAAGAGCACAGGCATACAAGCACAGTATATGTTCATATGTTACTTTTCTGAAGTTTGGAATAGATTTTAGTGCATTTCGCCTTAGTGTTTTTGATGTATATATGCATGAATCATTCGAGAT
GGAGGGGAGGTTCATACTGTATCTATCCAAGGAAGGCTTCATATTATGTATCATCATGACAAAGCTAATGTTTTGGAATAAACAGGAAGCTGAGTTGGGCATAATTGTATCTAAAGAATCATTTTTATGTATAGATAAATCAATTATACGGAAAAGATTGGAGATTAATATATCAAGATGTTAATTTTTGGAATTGATTA
ACCAGAGAAAATATGGAACAGTGGCGGTTTACTTGTAAATTGGATTCCTTCTGCTGCTACAATTAATTTTGCCATTTTATATGTTATGCAGCTCGATTCCTTGTAAATAGTGTG

>TRINITY_DN128981_c2_g2_i9 from sample A1 / Example unspliced isoform:

GCAAATCTGATTGAATTTCCAGAGGGATTGACTCAGACCAGTCAGGAAAAACGGAAAGAAAAGTTATCGGAGGTGGAACAGCAGTTGAAGAAAGCAGAGGCAGCTCAGAGACGCAAGATGCAAGTAGAGAAGGCTGCGAGTGAGATTCAGgtatgcatagctatctgtggtcgggttacatttgtaggattttctagtgg
gaatgtttttcatagataaaatttatctcaaatttttaacgtaactaagtgcttatgtgatcacagGCTGAGGCCATCAAGAAGATTCTGGGTCAAGACTCTTTGAGGAAGAAACGGGAAGATAAGCTACGGAAGCAAAGAGATGAGATGGCACAGGAGAAGGCTGCTAGTGCTATGATGCTTGCATCAAACAGTGTGAG
ATGGGTCATGGGACCTTCTGGCACAGTTGTTTCATTTTCTGAAAATACAGGGCTTCCGTGCATATTCAATTCTGGCCCATGCAGGTTTGATTTTACTTTCTAATTTCTTTTTATGATAGTATTTTTAGTCTTCAATCAGCCTTGAATTAGTATTACTAATTGGCAGCAATCATTTATTTTTCAAGCTACTTCCACAGAAG
ATGATTTTCTTTTCCCATCATTTATTGTTATTGTAGGTATCCTCCACCTCGTGAGAA