NA ----> NA

Intron 1 GTAAGCTAAC ... ACATTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.57	6	16	9
A2	61.54	5	14	2
A3	65.38	9	23	11
A4	87.5	2	20	8
A5	50	1	1	1
A6	73.05	19	70	33
A7	82.78	18	95	78
A8	85.86	14	95	75
A9	75	19	71	43
A10	80.68	20	95	72

>TRINITY_DN115577_c2_g2_i4 from sample A1 / Example spliced isoform

AAAGGTTTCAGTCCAGGATTTACCCAAAAACATGCTCATGCAAATAAAAAATGCTTTGGGCAAACCTAGTTTGCAAAACCATCAATGCTTGGTTGAGGATCATGGTTTGGAGTCTGTAAAAATATTAGCCTTCAAGAAGGTTAGCGAACCTGGCGATGTCCAACATTGTTTCAGCAGCATTTCAAAAGAGTGTGAAATAG
GGCAAGCAACCATGTCCGTGACTCGTTCGAAGCGAGGAAGATTGCAAGCCTTCCCCTCCAAATATTCAGACTCTGTATTGCAGCCATGGAAGAAAGTTTCAAGAAAGGTGCCCACTTGTCACTGTTCTTGAATTTCAGAGGGTTTCATTTTTTGGGGGGTG

>TRINITY_DN115577_c2_g2_i5 from sample A1 / Example unspliced isoform:

AAAGGTTTCAGTCCAGGATTTACCCAAAAACATGCTCATGCAAATAAAAAATGCTTTGGGCAAACCTAGTTTGCAAAACCATCAATGCTTGGTTGAGGATCATGGTTTGGAGTCTGTAAAAATATTAGCCTTCAAGAAGGTTAGCGAACCTGGCGATGTCCAACATTGTTTCAGCAGCATTTCAAAAGAGTGTGAAATAG
GGCAAGCAACCATGTCCGTGACTCGTTCGAAGCGAGGAAGATTGCAAGCCTTCCCCTCCAAATATTCAGACTCTGTATTGCAGCCATGGAAGAAAGTTTCAAGAAAGGTGCCCACTTGTCACTGTTCTTGAATTTCAGAGgtaagctaacgaagctcagggctcaaatgtaactcaatttttatgcactcacattctcac
tgtggttcgggaggtccaattacagcggctgataagcatttacattgtcagGGTTTCATTTTTTGGGGGGTGGGGTTTTGGGTTCAAATGTAACTCATAGGCACCCACATTCTCACTTTGGTTACAAAGGTCTCCGTCTACTTACAAGTTACAGCAACTGATATTCAGTTTCTGTAAGATTACAATTCTTCCAGGCTCCG
AATATTCAGACTCTATATTGCATCCATGGAAGAAAGTTTCAAGAAAGGTACCTACTTGTCACTGTTCTTGAATTTCAGAGGTAAGTTGATAAAGCTCACGGTTCAAATGTAACTAGATGACAGTCACATTTTCATTGTGATTTGGAAGGTCTGTAACAGTTAATAAAGCATTGCATTTAGGGTGACAATGTTTCAACTTT
TTCAGCTTTAGATTGCAAATGTAACTGAGACGTATTAAGCACCCACATTCTCGCTCTGGTTTAAAAGGTCCACTTACAAACTTATTAGCATCTTCAATAACAAAGGC

Intron 2 GTATGTTTCA ... ATCTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	8	4	5
A2	46.67	16	14	4.5
A3	33.7	21.7	11	10.8
A4	14.92	12.3	2.2	5.3
A5	16.67	5	1	1.2
A6	28.23	43.7	17.2	8.5
A7	28.38	116.8	46.3	33.5
A8	31.44	58.8	27	5.2
A9	41.98	73	52.8	23.2
A10	36.96	124.5	73.2	29.7

>TRINITY_DN115577_c2_g2_i2 from sample A1 / Example spliced isoform

CTTTATTTTTCGTCAATCTTGGTCTCACTCTGCAACTTCTCTACCTCGGTAAGTGTGACCTAAACTTCCAAGATCAGCACAATTCCAACCATTCTACAAATGTCCAAATTTATAAAAAAAAGCGAGAGGAGAAGCCAAGTTTCTGGACGTGAATACTGTTAAAGCAGCCCAACTCATCAGTTGCAGAGAATTGCGGATTT
CATAAAGTAATCCACAAATCGGGGACGCCATAGCCATCAGCAAGAGAGCACATATATTCCCCAACTTGATCATAACATCAATATTGCTCATTCTTCTCTATCAATGGATGATGTTTCTGCTAAGTTCCCAAATTGTTTAAGGGCATTTTTGGGAGACCAAGATTGTTCTCCTCCCCAAAATTCTGAGTGCCAAGAAAATC
TGAAGCTGCAAATGAATATCAAGGGCAGAAAAGAATCCATTCCATCCCTACATCCCATTCCCAGCATTCAACATTCTGTCAATGTCAGGACGTCCTCCAAGTCCCTTAAGATGGGCTCCCAGAAATCTCTACAATCTGTTATGAATACTCCTGTATTTTGTAGTGAAGAGAGAAATCAGGGGAATTCAAGTATAAGAAGC
CTCGATTTATCCAATCAAAAGATTGCAAGCCAGGAGAACAGTATGGGTGGATCTCTCTATTTGTTGGTAGATGCCATTGAGATATTAGAAAATCAGCAACATCACAGCAGTATCGGCATCGAGATTCAGGA

>TRINITY_DN115577_c2_g2_i3 from sample A1 / Example unspliced isoform:

CTTTATTTTTCGTCAATCTTGGTCTCACTCTGCAACTTCTCTACCTCGGTAAGTGTGACCTAAACTTCCAAGATCAGCACAATTCCAACCATTCTACAAATGTCCAAATTTATAAAAAAAAGCGAGAGGAGAAGCCAAGTTTCTGGACGTGAATACTGTTAAAGCAGCCCAACTCATCAGTTGCAGAGAATTGCGGATTT
CATAAAGTAATCCACAAATCGGGGACGCCATAGCCATCAGCAAGAGgtatgtttcgttcccaattattcttcaaacccacatcagttttcgacttcggaaaggaaagaatactgtattgattagtcaagaggagcgtacttttgtaagaagcactgatgagagattgactcgtggtaaccttacaacaaataaatgaata
gttaaggattactcttctttaacatctctgcagAGCACATATATTCCCCAACTTGATCATAACATCAATATTGCTCATTCTTCTCTATCAATGGATGATGTTTCTGCTAAGTTCCCAAATTGTTTAAGGGCATTTTTGGGAGACCAAGATTGTTCTCCTCCCCAAAATTCTGAGTGCCAAGAAAATCTGAAGCTGCAAAT
GAATATCAAGGGCAGAAAAGAATCCATTCCATCCCTACATCCCATTCCCAGCATTCAACATTCTGTCAATGTCAGGACGTCCTCCAAGTCCCTTAAGATGGGCTCCCAGAAATCTCTACAATCTGTTATGAATACTCCTGTATTTTGTAGTGAAGAGAGAAATCAGGGGAATTCAAGTATAAGAAGCCTCGATTTATCCA
ATCAAAAGATTGCAAGCCAGGAGAACAGTATGGGTGGATCTCTCTATTTGTTGGTAGATGCCATTGAGATATTAGAAGGTCAGCAACATCACAGACCTATCGGCATCGAGATTCAGGAAAAGGTGTCAGTCCAGGTTTTATCCAAAAACATGTTCATGCGAATAAAAAATGCTTTGGACAAACCTAGTTTGCAAAACCAT
CAATGCTTGGTTGAGGATCATGGTTTGGAGTCTGTAAAAATATTACCCTTCCATAAGGTTAGTGAACCTGGGGATGCCCAACATTGTTTCAGCAGCATTTCAAAAGAGTGTGAGATAGGGCAAGGAACCATGTCGGTGACTCGTTCGAAGCGAGGAAGATTGCAAGCCTTCCCCTCCAAATATTCAGACTCTGTATTGCA
GCCATGGAAGAAAGTTTCAAGAAAGGTGCCCACTTGTCACTGTTCTTGAATTTCAGAGGCTAAGCTAACGAAGCTCAGGACTCAAATGTAACTCGATGCTTATTATGCACTCACTTTCTCACTGTGGTTTTGGAGGTCCAATTACAGCAACTGATAAGCATTTACATGGTAAGGGTTTCATTTGGGGGTGTAAGCTAACG
AAGCTCAGGGCTCAAATGTAACTCAATTTTTATGCACTCACATTCTCACTGTGGTTCGGGAGGTCCAATTACAGCGGCTGATAAGCATTTACATTGTCAGGGTTTCATTTTTTGGGGGGTGGGGTTTTGGGTTCAAATGTAACTCATAGGCACCCATATTCTCACTATGTTTTCAAAAGTCTACTTACAGTAACTGATTT
ACTGATAAAAACGAGGAAGCTTACAAGCCTTCCCTCCGAATATTCAGACTCTATATTGCATCCATGGAAGAAAGTTTCAAGAAAGGTACCTACTTGTCACTGTTCTTGAATTTCAGAGGTAAGTTGATGAAGCTCAAGGTTCAAATGTAACTAGATGACTCATGACACTCACATTTTCGTTGTTATTTAGAAGGTTTGTA
ACAGTTAATAAGCC