sp|Q42538|SAT5_ARATH ----> Serine acetyltransferase 5

Intron 1 GTATGTTCAT ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.89	11	7	7
A2	55.56	4	4	6
A3	11.11	28	3	4
A4	28	9	2	5
A5	17.65	7	0	3
A6	2.33	21	0	1
A7	14	43	1	13
A8	12.38	46	3	10
A9	7.46	62	1	9
A10	15.62	27	5	5

>TRINITY_DN48047_c0_g1_i21 from sample A7 / Example spliced isoform

TAATAGTTTTATTGAGCTGCTTCCACGAAGTGTTTGTATATTCTGCTGGAAGTATTGATGTAATTTATAATAGTTTTATTGAGCTGCTTCCACGAAGTGTTTGTATATTCTGCTGGAAGTATTGATGTAATTTATAATAGTTTTATTGAGCTGCTTCTTCATTTGTATATTCTGCTGGAAGTATTGATGTAATTTTTAAT
TTTTAATGTATATTCTGCTTATTGGCTTTCATATCTGTTATTAGTTTGTTGTCTTCCTGCAGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATATGGTTCCATCATCTATGTGCTGAGGTTTTCTTTTTTAAGTGGTTGCGAAGATGAAAT
GACACCCCGCCGGAGAAAGAGAAACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGTTCCAAGGTTTGCTGAAGGAGCTGGAAATTGATATTAATACATTAAGTAAAGGGAAGCTAGTACTCAACAATGCAGAAGGGAAGCCCAAAAATAATATCAGCACATAAGTGTTGGAAAAGAAAATTGCACAGAACAA
GGTGAAGATGGAAAAAATGACCTCTAACATGAAAATAAAAAATGATCTCAAGACCGTGGCATTGGAAACTTTTAAGATCAATTACTTGGATGCAAGAATTTCAGTTGCCTGGTGCAAATGGCATGAATTTTGAATTGTAGAGCTAACTATTCCTTTTCTGTAGTTTTCTATGGTATCATAAAGGCTATTGGACAATATAT
CTATTTTGGAACTTTGAGTTGGATGACAGTGAATGTTGGATCACCTTATATGTGTTTTCTTTTGTTATTTAGTTTGAATTAACTGGAGCTGAGTTGGAATCAATAGTGGAGTGCACTTTAAAAGATAGTGATTTTTGTGATGTTCAGGATTGTTTGTCTATCTAGTATTTTGAATTCTTTTTATACAAAGAGCTACAAAC
TCTATATTTTACTGGAAGTGTTTATTGTTTAAGAATATTTAGAAAC

>TRINITY_DN48047_c0_g1_i3 from sample A7 / Example unspliced isoform:

TGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATATGGTTCCATCATCTATGTGCTGAGGTTTTCTTTTTTAAGgtatgttcatattttgttcatacttaaatttcttttgcagtgtaataatagttaaaaaatttttgcagtgtattaatagttttattttgattttggatggagtagtttcactaatatt
aggatttaggatttatactagtgtagtaattttttattttcattttggatggagtagtttagttttattttcattttttgatcactgatattaggatttaggatttattgcagtgttgtaatagttttattttcattttttgataaagtaattttttatataatatagcagaattcgaaacctatagaaatagtgttata
ggtaatatttctatgttttataggtgatatttcaggtccatacctggtatgtataaaatggtaagagttgtagtaagagttctttcatgttctaaatgaagttaaatacattatttttatttcaattttggaatgctgtgggaaattgcaagagttcattgttattttgatttcagagtgaagggcatttcaatcaagtg
ataattccagataaagctagtttgcatttcattctaacaaattttctttgttattgattttggcatagttaacaatccgaaatgaattctaatttgaaatagattaaaaaaaagggttttaccttttagtttcaacctatgaactgcctgatttctttcctacgttagtttgttttttctttcctaggttagtttgtagg
ttagtttgtttgcctgggttgtttatatcaatctttgtttgcagTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAAACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGTTCCAAGGTTTGCTGAAGGAGCTGGAAATTGATATTAATACATTAAGTAAAGGGAAGCTAGTACTCAACAATGCA
GAAGGGAAGCCCAAAAATAATATCAGCACATAAGTGTTGGAAAAGAAAATTGCACAGAACAAGGTGAAGATGGAAAAAATGACCTCTAACATGAAAATAAAAAATGATCTCAAGACCGTGGCATTGGAAACTTTTAAGATCAATTACTTGGATGCAAGAATTTCAGTTGCCTGGTGCAAATGGCATGAATTTTGAATTGT
AGAGCTAACTATTCCTTTTCTGTAGTTTTCTATGGTATCATAAAGGCTATTGGACAATATATCTATTTTGGAACTTTGAGTTGGATGACAGTGAATGTTGGATCACCTTATATGTGTTTTCTTTTGTTATTTAGTTTGAATTAACTGGAGCTGAGTTGGAATCAATAGTGGAGTGCACTTTAAAAGATAGTGATTTTTGT
GATGTTCAGGATTGTTTGTCTATCTAGTATTTTGAATTCTTTTTATACAAAGAGCTACAAACTCTATATTTTACTGGAAGTGTTTATTGTTTAAGAATATTTAGAAAC

Intron 2 GTATGTTCAT ... TTTGGCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	20	16
A2	100	0	18	3
A3	69.7	5	15	8
A4	76.47	2	10	3
A5	100	0	5	1
A6	60	4	5	7
A7	74.55	7	12	29
A8	80	6	25	23
A9	100	0	17	15
A10	80.49	4	23	10

>TRINITY_DN48851_c2_g1_i20 from sample A10 / Example spliced isoform

TGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGTTAACAATCCGAAATGAATTCTAATTTGAAATAGATTAAACAAAAGGGGTTTGCAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATCGAAAGAAA
CGAGATAGAATTGAATCAGTGCACATAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAACCCTCCTAGAAGGGTTTAGGGATTTGTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCTTTCCTCGATTCGGCTTGCATTTTCAGCCACAGATTACCTGATGTAAGCCC
CTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGGACGGTGCTTGAAAGAGTGGAGTTTGGAAGCCAGGGCACTTTCCAGAGATTTGTGAAGACCGTAGTGCAGAGAAAGCCTCCTAGAGAAAGGCACCTCCGATCTCAAGTTGAGGGGATCTCCG
TGTCTCCTTCCATTCCACCAACTTTCTGGTTGGTTTCGCTCTGGTTGGTTATAACGTGGATCTCTACAAAGTCTGCGTTATATAAAGGAGATTTACAGAGTTTCGTAAAAAGAAGTCACCGTCAAAATCCAGCCTGTACTTTTTCGGTATGGTAGGAATGTGACATTGTGATTATTAAATTGCAAAGCTAATTGTTTTGA
TTGATTTTCTGGTTTCCGTATGTTTCGAAAAGCTGAAGTTCTTGCCTTATTAGGAAGATTAAGGTGTCAGTTCTTGTCCTATTAGAACGGTTAGC

>TRINITY_DN48851_c2_g1_i19 from sample A10 / Example unspliced isoform:

AATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGgtatgttcatattttgttgatactaaattttttgcagtgtaataataattttattttcattttggatggagtagtttcact
gatattaggatttaggatttatagcagtgtagtaataattttattttcatgttttgataaagtacatttttgatataatatagcagaatcgaagatctaaacctatagaaatagtgtgataggtgatatttctatgtttgataagtgatatttcaggtccattacctggtatgtataaaatggtaagagttgtagtaaga
gttctttcatgttctaaataaagttaaataatttatttttatttcaatttttgaatgctgtgggaaattgtaaaagttcattgttatttagatttcaaagtgaagggcatttcaatcaagtggtaattccagataaagctagtttacatttcattctaacaaattttctttgttattgattttggcatagTTAACAATCC
GAAATGAATTCTAATTTGAAATAGATTAAACAAAAGGGGTTTGCAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGTGCACATAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAACCCTCCTAGAAGGGTTTAGGGATTT
GTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCTTTCCTCGATTCGGCTTGCATTTTCAGCCACAGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGGACGGTGCTTGAAA
GAGTGGAGTTTGGAAGCCAGGGCACTTTCCAGAGATTTGTGAAGACCGTAGTGCAGAGAAAGCCTCCTAGAGAAAGGCACCTCCGATCTCAAGTTGAGGGGATCTCCGTGTCTCCTTCCATTCCACCAACTTTCTGGTTGGTTTCGCTCTGGTTGGTTATAACGTGGATCTCTACAAAGTCTGCGTTATATAAAGGAGAT
TTACAGAGTTTCGTAAAAAGAAGTCACCGTCAAAATCCAGCCTGTACTTTTTCGGTATGGTAGGAATGTGACATTGTGATTATTAAATTGCAAAGCTAATTGTTTTGATTGATTTTCTGGTTTCCGTATGTTTCGAAAAGCTGAAGTTCTTGCCTTATTAGGAAGATTAAGGTGTCAGTTCTTGTCCTATTAGAACGGTT
AGC

Intron 3 GTAAGGTTTA ... ATAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.37	16	38	15
A2	70.37	8	19	9
A3	62.22	17	28	4
A4	70.21	14	33	11
A5	63.64	4	7	2
A6	44.83	32	26	0
A7	41.35	61	43	1
A8	46.07	48	41	6
A9	45.79	58	49	2
A10	47.52	53	48	4

>TRINITY_DN46662_c2_g1_i5 from sample A8 / Example spliced isoform

GTTGAATCCTGAGTTATATTTGGTTTCCAGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGGGTTTCTTTTCCCTGGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGCTGGACTTTGGTTTCAGTCTTCAACTATGGATGAACGCTAACCCGTTTCGGAGC
TACATGATCTTTTAAAACCATGGATGTCCGGGTTGCCGACGACATGGTATCCTTTTAACAAGTTACGACCTAGCTTATTGCGTA

>TRINITY_DN46662_c2_g1_i7 from sample A8 / Example unspliced isoform:

AACGGTGATTTATCGGAAGAAAAATCCTGTAAGCTAGCCTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATGCAGGCCAGGGGAGTGCTTCCTTACATAGAACCGACTTGGCCGATGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGAT
CGCCTCTGCTATATCTGCCCGAATGCGCCAGTGGCCTTGGCGCGTTCGAATTCCAACCATATGGAGGGACTGCTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTG
ACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGGATTGAATTCATATCAAATTTCCTTTGCATTATGTATAGAAGTTGAATCCTGAGTTATATTTGGTTTCCAGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGG
GTTTCTTTTCCCTGGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGCTGGACTTTGGTTTCAGTCTTCAACTATGGATGAACGCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGgtaaggtttacctacacacatttgttttagggcattaattttacagtttg
gtcttcatattccaacgatacttaactacttataataattgcagGATGTCCGGGTTGCCGACGACATGGTATCCTTTTAACAAGTTACGACCTAGCTTATTGCGTA

Intron 4 GTATGTTCAT ... TAAACAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.39	4.6	20	13.9
A2	68.34	5.6	18.7	5.7
A3	45.01	14.4	15.5	8
A4	34.21	14.4	10	4.4
A5	46.58	4.4	7.8	0
A6	26.18	22.2	4.9	11
A7	33.03	43.7	11.2	31.5
A8	37.29	45.6	28.6	24.7
A9	23.52	50.8	18.2	12.9
A10	44.24	26.8	26.4	15.8

>TRINITY_DN45556_c1_g1_i11 from sample A6 / Example spliced isoform

GTTGAATCCTGAGTTATATTTGGTTTCCAGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGGGTTTCTTTTCCCTTGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGCTGGACTTTGGTTTCAGTCTTCAACTATGGATGAACCCTAACCCGTTTCGGAGC
TACATGATCTTTTAAAACCATGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGGGGTTTGCAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGA
AGAATCGAAAGAAACGAGATAGAATTGAATCAGTGCACATAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAACCCTCCTAGAAGGGTTTAGGGATTTGTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCTTTCCTCGATTCGGCTTGCATTTTCAGCCACAGATTA
CCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGGACGGTGCTTGAAAGAGTGGAGTTTGGAAGCCAGGGCACTTTCCAGAGATTTGTGAAGACCGTAGTGCAGAGAAAGCCTCCTAGAGAAAGGCACCTCCGATCTCAAGT
TGAGGGGATCTCCGTGTCTCCTTCCATTCCACCAACTTTCTGGTTGGTTTCGCTCTGGTTGGTTATAACGTGGATCTCTACAAAGTCTGCGTTATATAAAGGAGATTTACAGAGTTTCGTAAAAAGAAGTCACCGTCAAAATCCAGCCTGTACTTTTTCGGTATGGTAGGAATGTGACATTGTGATTATTAAATTGCAAA
GCTAATTGTTTTGATTGATTTTCTGGTTTCCGTATGTTTCGAAAAGCTGAAGTTCTTGCCTTATTAGGAAGATTAAGGTGTCAGTTCTTGTCCTATTAGAACGGTTAGTACCCACGGAAACGTAAAGATTTTATAAAATGGTATTTCTCAACAAGTCAAATCTATATTTAAATGAGTTCTTGATGGCATGGCAAAACATT
ATATATAAAAACAAAACCACCTGCCATGTGATTCGATTATTATATTTTAAATACAGCTGGCATGGAAAACAACAACCCAACTGCAATTTAGTGTTGCTTTTAATATGCAACTCAACATTTTCCACGGGCATGGAATACAGCCGGTCAACTTTTAGAATCCGTTACATGCTTATTCTAAATAATCTGAAAATTAGAACGC

>TRINITY_DN45556_c1_g1_i2 from sample A6 / Example unspliced isoform:

GTTTCTTTTCCCTTGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGCTGGACTTTGGTTTCAGTCTTCAACTATGGATGAACCCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATC
GTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGgtatgttcatattttgttgatactaaattttttgcagtgtaataataattttattttcattttggatggagtagtttcactgatattaggatttaggatttatagcagtgtagtaataattttattttca
tgttttgataaagtacatttttgatataatatagcagaatcgaagatctaaacctatagaaatagtgtgataggtgatatttctatgtttgataagtgatatttcaggtccattacctggtatgtataaaatggtaagagttgtagtaagagttctttcatgttctaaataaagttaaataatttatttttatttcaatt
tttgaatgctgtgggaaattgtaaaagttcattgttatttagatttcaaagtgaagggcatttcaatcaagtggtaattccagataaagctagtttacatttcattctaacaaattttctttgttattgattttggcatagttaacaatccgaaatgaattctaatttgaaatagattaaacaaaagGGGTTTGCAGTGG
TCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGGTAGATGTACATTTTTTCAAGGTCTTTATTAAACTGTTAATGTATAGAAATTAGAATTGAATCAGGTA

Intron 5 GTATGTTCAT ... GGGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.37	3	20	15
A2	78.95	4	19	11
A3	39.39	20	16	10
A4	50	6	10	2
A5	60	4	9	3
A6	36.84	18	6	15
A7	40.19	32	12	31
A8	50.41	30	31	30
A9	28.57	45	19	17
A10	58.9	15	27	16

>TRINITY_DN45556_c1_g1_i13 from sample A6 / Example spliced isoform

CTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCA
TCGGCAGAGAAGAGGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAAT

>TRINITY_DN45556_c1_g1_i2 from sample A6 / Example unspliced isoform:

GTTTCTTTTCCCTTGGTCGGTGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGCTGGACTTTGGTTTCAGTCTTCAACTATGGATGAACCCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATC
GTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGgtatgttcatattttgttgatactaaattttttgcagtgtaataataattttattttcattttggatggagtagtttcactgatattaggatttaggatttatagcagtgtagtaataattttattttca
tgttttgataaagtacatttttgatataatatagcagaatcgaagatctaaacctatagaaatagtgtgataggtgatatttctatgtttgataagtgatatttcaggtccattacctggtatgtataaaatggtaagagttgtagtaagagttctttcatgttctaaataaagttaaataatttatttttatttcaatt
tttgaatgctgtgggaaattgtaaaagttcattgttatttagatttcaaagtgaagggcatttcaatcaagtggtaattccagataaagctagtttacatttcattctaacaaattttctttgttattgattttggcatagttaacaatccgaaatgaattctaatttgaaatagattaaacaaaaggggtttgcagTGG
TCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGGTAGATGTACATTTTTTCAAGGTCTTTATTAAACTGTTAATGTATAGAAATTAGAATTGAATCAGGTA

Intron 6 GTATGGTTTC ... TGTATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.75	9	1	7
A2	20.83	19	0	5
A3	14.29	12	1	2
A4	41.67	7	2	5
A5	0	1	1	0
A6	26.19	31	1	11
A7	26.4	92	6	33
A8	28.33	129	11	51
A9	22.29	122	3	35
A10	27.37	69	10	26

>TRINITY_DN48047_c0_g1_i21 from sample A7 / Example spliced isoform

TAATAGTTTTATTGAGCTGCTTCCACGAAGTGTTTGTATATTCTGCTGGAAGTATTGATGTAATTTATAATAGTTTTATTGAGCTGCTTCCACGAAGTGTTTGTATATTCTGCTGGAAGTATTGATGTAATTTATAATAGTTTTATTGAGCTGCTTCTTCATTTGTATATTCTGCTGGAAGTATTGATGTAATTTTTAAT
TTTTAATGTATATTCTGCTTATTGGCTTTCATATCTGTTATTAGTTTGTTGTCTTCCTGCAGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATATGGTTCCATCATCTATGTGCTGAGGTTTTCTTTTTTAAGTGGTTGCGAAGATGAAAT
GACACCCCGCCGGAGAAAGAGAAACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGTTCCAAGGTTTGCTGAAGGAGCTGGAAATTGATATTAATACATTAAGTAAAGGGAAGCTAGTACTCAACAATGCAGAAGGGAAGCCCAAAAATAATATCAGCACATAAGTGTTGGAAAAGAAAATTGCACAGAACAA
GGTGAAGATGGAAAAAATGACCTCTAACATGAAAATAAAAAATGATCTCAAGACCGTGGCATTGGAAACTTTTAAGATCAATTACTTGGATGCAAGAATTTCAGTTGCCTGGTGCAAATGGCATGAATTTTGAATTGTAGAGCTAACTATTCCTTTTCTGTAGTTTTCTATGGTATCATAAAGGCTATTGGACAATATAT
CTATTTTGGAACTTTGAGTTGGATGACAGTGAATGTTGGATCACCTTATATGTGTTTTCTTTTGTTATTTAGTTTGAATTAACTGGAGCTGAGTTGGAATCAATAGTGGAGTGCACTTTAAAAGATAGTGATTTTTGTGATGTTCAGGATTGTTTGTCTATCTAGTATTTTGAATTCTTTTTATACAAAGAGCTACAAAC
TCTATATTTTACTGGAAGTGTTTATTGTTTAAGAATATTTAGAAAC

>TRINITY_DN48047_c0_g1_i4 from sample A7 / Example unspliced isoform:

CATCTTTAACATTTTGATTCTATTAGTTCCAAGGTTTGCTGAAGGAGCTGGAAATTGATATTAATACATTAAGTAAAGGGAAGCTAGTACTCAACAATGCAGAAGGGAAGCCCAAAAATAATATCAGCACATAAGTgtatggtttcttacgtgcaattattggaacctatatttatgctttttgttgttgcttgtagtgt
acatggtaatttctggtatgataggttacaactttattgtattgcagGTTGGAAAAGAAAATTGCACAGAACAAGGTGAAGATGGAAAAAATGACCTCTAACATGAAAATAAAAAATGATCTCAAGACCGTGGCATTGGAAACTTTTAAGATCAATTACTTGGATGCAAGAATTTCAGTTGCCTGGTGCAAATGGCATGA
ATTTTGAATTGTAGAGCTAACTATTCCTTTTCTGTAGTTTTCTATGGTATCATAAAGGCTATTGGACAATATATCT

Intron 7 GTGAGTCTCA ... TTGCCGCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.22	30	48	26
A2	48.91	47	66	24
A3	45.34	44	64	9
A4	47.29	34	47	14
A5	50	14	22	6
A6	30.91	57	33	18
A7	32.78	122	57	62
A8	29.88	88	49	26
A9	36.03	87	52	46
A10	34.18	104	58	50

>TRINITY_DN46662_c2_g1_i19 from sample A8 / Example spliced isoform

AACGGTGATTTATCGGAAGAAAAATCCTGTAAGCTAGCCGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAAAGAGTGGAGTTTGGAAGCTTACTGCTCAGAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCAG
TTGGTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATGCAGGCCAGGGGAGTGCTTCCTTACATAGAACCGACTTGGCCGATGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCCGAATGCGCCAGTGGC
CTTGGCGCGTTCGAATTCCAACCATATGGAGGGACTGCTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAAT
TCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGGGGTTTGCAGTGGTCGTGAAGATGAAAT
GACACCCCGCCGGAGAAAGAGAAACCATGAAGAATCGAAAGAAACGAGATGAGAATTGAATCAGGTAGATGTACATTTTTGCACATAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCTTTCCTCGATTCGGCTTGCATTTTCAGCCACAGATTACCTGAT
GTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAAAGAGTGGAGTTTGGAAGCTTACTGCTCAGAAAGCCTCCTAGAAGGGGCTCGCTTGGGGACATTTTGCTTTGCCTTCGCGCCGGCCACCTCCGATCTATCTATCATGGGCAATAT
ACAGCAGCGGGGGATCCGTTTCTCCTTCGATTCCACCAACGCTCTGGTTGGTTTCGCTCTAAACCAGTGCATAATATTATAACCATAGTCTGCGTTATATAAAGGAGATTTGTATGTAATTATCGTAAAAAGAAGTCACCGTCCAAATCCAGCCTGTACATGTTTGGTTTATTAAATTGCAAAGCTAATTGTTTTGATTG
ATTTTCTGGTTTCCGTATGTTTCGAAAAGCTGAAGTTCTTGCCTTATTAGGAAGATTAAGATGTCAGTTCTTGTCTTATTAGAAAGGTTAGTACCCACGGAAACGTAAAGATTTTATAAAATGGTATTTCTCAACAAGTCAAATCTATATTTAAATGAGTTCTTGATGGCATGGCAAAACATTATATATAAAAACAAAAC
CACCTGCCATGTGATTCGATTATTATATTTTAAATACAGCTGGCATGGAAAACAACAACCCAACTGCAATTTAGTGTTGCTTTTAATATGCAACACAGCCGGTCAACTTTTAGAATCCGTTACATGCTTATTCTAAATAATGTGAAAATTACAACTCTTCACCAACCTTGAAACTTGGAAACTCATTCATCACTATTCTA
TTAAAATATTGATTTTCCTTTCCTCCAAATGGGAGACACGTCTCTCTCAACCTAACAATTTAAAACTCATTTAAGGCCATATATATATTATTTTTATTATTAAAAGCAAATGATTGAATTTTTTTTTCCCAA

>TRINITY_DN46662_c2_g1_i30 from sample A8 / Example unspliced isoform:

CGGTGATTTATCAAAAGAAAAATCCTTTAAGCTAGCGGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAAAGAGTGGAGTTTGGAAGCTTACTGCTCAGAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCAGTT
GGTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATACAGGCCAGGGGAGTGCTACCTTACATAGAACCGACTTCGCCGATGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCCGAATGCGCCAGTGGCCT
TgtgagtctcagaagtcttgatgttgcttcaaatcacctcaaatctctttctgaagcttaatcgttactattcctgagagcatactaaattgccgctagGGCGCGTTCGAATTCCAACCATATGGAGGGACTGCTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACA
TGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGT
GGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGGGGTTTGCAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAAACCATGAAGAATCGAAAGAAACGAGATGAGAATTGAATCAGGTAGATGTACATTTTTGCACATAAAGCCAGGGATTTGTGA
GCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCTTTCCTCGATTCGGCTTGCATTTTCAGCCACAGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAAAGAGTGGAGTTTGGAAGC
TTACTGCTCAGAAAGCCTCCTAGAAGGGGCTCGCTTGGGGACATTTTGCTTTGCCTTCGCGCCGGCCACCTCCGATCTATCTATCATGGGCAATATACAGCAGCGGGGGATCCGTTTCTCCTTCGATTCCACCAACGCTCTGGTTGGTTTCGCTCTAAACCAGTGCATAACTATCTAGGGCCTGTTTAGCATACAACATA
ATATTAATATTATAACCATAGTCTGCGTTATATAAAAGAAATTTGTATGTAATTATCGTAAAAAGAAGTCACCGTCCAAATCCAGCCTGTACATGTTTGGTATGGTAGGAATGTGACATTGTGATTATTAAATTGCAAAGCTAATTGTTTTGATTGATTTTCTGGTTTCCGTATGTTTCGAGAAGCTGAAGTTCTTGCCT
TATCATGAAGATTAAGATGTCAGTTCTTGTCTTATTAGAAAGGTTAGTACCCACGGAAACGTAAAGATTTTATAAAATGGTATTTCTCAACAAGTCAAATCTATATTTAAATGAGTTCTTGATGGCATGGCAAAACATTATATATAAAAACAAAACCACCTGCCATGTGATTCGATTATTATATTTTAAATACAGCTGGC
ATGGAAAACAACAACCCAACTGCAATTTAGTGTTGCTTTTAATATGCAAC

Intron 8 GTGAGTCTCA ... GAATCTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.46	5	18	29
A2	94.52	2	26	43
A3	97.83	1	51	39
A4	100	0	28	40
A5	100	0	12	26
A6	100	0	10	2
A7	100	0	9	40
A8	76.27	7	19	26
A9	92.59	2	12	38
A10	79.31	6	19	27

>TRINITY_DN46662_c2_g1_i16 from sample A8 / Example spliced isoform

AACGGTGATTTATCGGAAGAAAAATCCTGTAAGCTAGCCTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATACAGGCCAGGGGAGTGCTACCTTACATAGAACAGACTTGGCCGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCCGA
ATGCGCCAGTGGCCTTAATCTGTACATTCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAAC

>TRINITY_DN46662_c2_g1_i11 from sample A8 / Example unspliced isoform:

AACGGTGATTTATCGGAAGAAAAATCCTGTAAGCTAGCCTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATACAGGCCAGGGGAGTGCTACCTTACATAGAACAGACTTGGCCGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCCGA
ATGCGCCAGTGGCCTTgtgagtctcagaagtcttgatgttgcttcaaatcacctcaaatctctttctgaagcttaatccttactatgttgaatttatgaatcgttgttgggttatagattcaatttctgaatcgttgttgaatttcttatgttgaatttatgaatcgttgttggctctgtgtttataaaatattaaattg
ctggtgatctttttaatgatctagagactggaaaatatatatcggtttggtcttggatccgttctcgtgaactttatgtcttttcttatgttgaatttatgaatcgttgttgggttagattcaatttctgataggttgaattcctgatgggttgaatttctgaatcgttgttgtatgatatgaatttaacggcgcgttcg
aatttcaaacatctggagggactacttgatacgctgggtttgatattgatgaatctgaagAATCTGTACATTCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACAGTCCTGAAGCCGAACGACGTGGGTGGCACTCAAGTACGTCTGTGACTAGTAATCG

Intron 9 GTATGTTCAT ... TTTGAAATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	94.44	1	20	14
A2	78.57	3	18	4
A3	58.43	8	14.5	8
A4	66.67	3	10	2
A5	29.41	6	5	0
A6	42.11	11	5	11
A7	46.67	24	12	30
A8	52.48	24	25	28
A9	44.44	20	17	15
A10	43.82	25	23	16

>TRINITY_DN48851_c2_g1_i5 from sample A10 / Example spliced isoform

TTTTTAATGTATATTCTGCTTATTGGCTTTCATATCTGTTATTAGTTTGTTGTCTTCCTGCAGTAATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGATTAAACAAAAGGGGTT
TGCAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGT

>TRINITY_DN48851_c2_g1_i19 from sample A10 / Example unspliced isoform:

AATTAAATCGTACCTGTCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATACGGTGCCATCATCTATGTGCTGAAGTTTTCTTTTTTAAGgtatgttcatattttgttgatactaaattttttgcagtgtaataataattttattttcattttggatggagtagtttcact
gatattaggatttaggatttatagcagtgtagtaataattttattttcatgttttgataaagtacatttttgatataatatagcagaatcgaagatctaaacctatagaaatagtgtgataggtgatatttctatgtttgataagtgatatttcaggtccattacctggtatgtataaaatggtaagagttgtagtaaga
gttctttcatgttctaaataaagttaaataatttatttttatttcaatttttgaatgctgtgggaaattgtaaaagttcattgttatttagatttcaaagtgaagggcatttcaatcaagtggtaattccagataaagctagtttacatttcattctaacaaattttctttgttattgattttggcatagttaacaatcc
gaaatgaattctaatttgaaatagATTAAACAAAAGGGGTTTGCAGTGGTCGTGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATCGAAAGAAACGAGATAGAATTGAATCAGTGCACATAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAACCCTCCTAGAAGGGTTTAGGGATTT
GTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGCTCTCTGCATCTTTCCTCGATTCGGCTTGCATTTTCAGCCACAGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCGCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGGACGGTGCTTGAAA
GAGTGGAGTTTGGAAGCCAGGGCACTTTCCAGAGATTTGTGAAGACCGTAGTGCAGAGAAAGCCTCCTAGAGAAAGGCACCTCCGATCTCAAGTTGAGGGGATCTCCGTGTCTCCTTCCATTCCACCAACTTTCTGGTTGGTTTCGCTCTGGTTGGTTATAACGTGGATCTCTACAAAGTCTGCGTTATATAAAGGAGAT
TTACAGAGTTTCGTAAAAAGAAGTCACCGTCAAAATCCAGCCTGTACTTTTTCGGTATGGTAGGAATGTGACATTGTGATTATTAAATTGCAAAGCTAATTGTTTTGATTGATTTTCTGGTTTCCGTATGTTTCGAAAAGCTGAAGTTCTTGCCTTATTAGGAAGATTAAGGTGTCAGTTCTTGTCCTATTAGAACGGTT
AGC

Intron 10 GTAGATGTAC ... ATCCTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.42	11	32	14
A2	93.33	2	28	6
A3	85	6	34	10
A4	87.5	3	21	6
A5	76.92	3	10	1
A6	68.42	12	26	12
A7	71.91	25	64	24
A8	64.1	28	50	16
A9	58.49	22	31	6
A10	65.57	21	40	10

>TRINITY_DN100139_c1_g1_i10 from sample A4 / Example spliced isoform

TTTAAACGTAATGGAAATACAAACATCTTGTGAACAGACTTTGTTAAAAGAAATTATTGACAATGTGCTCTTGTTTTTCTGAGTTGCAGTAGAGATGGAATGCCAGAAATTGGCGAGTAAAGGCATAAATATAGTACAAAGCTGGTGCCCTGGAAGATGAAAGAAGGATTGAAGCATGGCTATGTAACGGTATGCGATTA
CGTTGTAATATTTGAAGCATATTTTCAGCGAGATCGAGACATTCTGGGCAGAACGATTCTATTCTCAGAATTGGCCTTAGTCCAAGCTTGCTGGAAGTTTGTAGAGATGGAATGCTAGAATGACGTTGTAATATTTGAAGCATATTTGCAGCCCGATGGAGACATTCTGGGCAGAACGATTCCATTCTCAGTGCACAATT
CAGAATTGGCCTTTGTTCAAGTGGCTGGAAGTTTGTTGTCTTCGTACAAGCCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATCATTCTATAAAGGCATATGGTGCCATCATCTATGTGATGAGCTTTTATTTTTTAAGTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATG
AAGAATAGAAAGAAACGAGATGGAATTGAATCAGAGATTCATATCGGCCAGCTATAACGAACGGTACCTCTGAAACCCTATTCCGAAGTGCAAGGGCGAGAGAAAGGTTATGCGCCACTCTGTATATAAGAAAGAGCAGGCAATGTATATAAGAAACGCATGCCCTAAGATCCCTCTTATTGAATCTTGGATCTCTGCAT
CTTCCTCCACAACACCCATTATGAGCTAAGACCGTAGTGCACAGAAAGCCTGGACTAGAAGGGGCTCGCTCTCTGCA

>TRINITY_DN100139_c1_g1_i8 from sample A4 / Example unspliced isoform:

TTTAAACGTAATGGAAATACAAACATCTTGTGAACAGACTTTGTTAAAAGAAATTATTGACAATGTGCTCTTGTTTTTCTGAGTTGCAGTAGAGATGGAATGCCAGAAATTGGCGAGTAAAGGCATAAATATAGTACAAAGCTGGTGCCCTGGAAGATGAAAGAAGGATTGAAGCATGGCTATGTAACGGTATGCGATTA
CGTTGTAATATTTGAAGCATATTTTCAGCGAGATCGAGACATTCTGGGCAGAACGATTCTATTCTCAGAATTGGCCTTAGTCCAAGCTTGCTGGAAGTTTGTAGAGATGGAATGCTAGAATGACGTTGTAATATTTGAAGCATATTTGCAGCCCGATGGAGACATTCTGGGCAGAACGATTCCATTCTCAGTGCACAATT
CAGAATTGGCCTTTGTTCAAGTGGCTGGAAGTTTGTTGTCTTCGTACAAGCCCAATATGTATAAGAACGCCTGGAATTAGATCGTGGATGACTTCATCATTCTATAAAGGCATATGGTGCCATCATCTATGTGATGAGCTTTTATTTTTTAAGTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATG
AAGAATAGAAAGAAACGAGATGGAATTGAATCAGgtagatgtacattttctcaaggtctttattaaattgttaatgtatagaaattagaattgaaacaggtagatgtacattgttctacttattgcctagtcttctattgaaatagctctagctagttattaaaactagctttagcttattgttgaaattatttgaaatt
atttagctgacattattaaaattcaaaatctatttggctcaaatttcactagatgactaagttatccttccagAGATTCATATCGGCCAGCTATAACGAACGGTACCTCTGAAACCCTATTCCGAAGTGCAAGGGCGAGAGAAAGCTTATGCGCCACTCTATATATAAGAAAGCCCTTAAAATTGAGCAGGCAATGTATA
TAAGAAACGCATGCCCTAAGATCCCTCTTATTGAATCTTGGATCTCTGCATCTTCCTCCACAACACCCATTATGAG

Intron 11 GTAGATGTAC ... TATCGGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.82	4.3	30.3	10
A2	77.61	6	30.7	11
A3	76.86	7.3	37.7	11
A4	75.19	5	22.3	8
A5	55.01	5.3	9	4
A6	54.66	20.3	28	21
A7	61.09	29.3	64.7	27
A8	56.7	29	54.3	21
A9	41.62	25.3	26	10
A10	51.72	26.3	39.3	17

>TRINITY_DN131089_c4_g1_i4 from sample A1 / Example spliced isoform

GGATGACTTCATCATTCTATAAAGGCATATGGTGCCATCATCTATGTGATGAGGTTTTATTTTTTAAGTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATAGAAAGAAACGAGATGGAATTGAATCAGCTATAACGAACGGTACCTCTGAAACCCTATTCCGAAGTGCAAGGGCGAGAG
AAAGCTTATGCGCCACTCTATATATAAGAAAGCCCTTAAAATTGAGCAGGCAATGTATATAAGAAACGCATGCCCTAAGATCCCTCTTATTGAATCTTGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGCTAAGACCGTAGTGCACAGAAAGCCTGGACTAGAAGGGGCTCGCTCTCTGCATCAGTTGGAAAGC
CAGGGATTTGTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGC

>TRINITY_DN131089_c4_g1_i11 from sample A1 / Example unspliced isoform:

TGATACTAAATTTTTTGCAGTGTAATAATAATTTTATTTTCATTTTGGATGGAGTAGTTTCACTGATATTAGGATTTAGGATTTATAGCAGTGTAGTAATAATTTTATTTTCATGTTTTGATAAAGTACATTTTTGATATAATATAGCAGAATCGAAGATCTAAACCTATAGAAATAGTGTGATAGGTGATATTTCTATG
TTTGATAAGTGATATTTCAGGTCCATTACCTGGTATGTATAAAATGGTAAGAGTTGTAGTAAGAGTTCTTTCATGTTCTAAATAAAGTTAAATAATTTATTTTTATTTCAATTTTTGAATGCTGTGGGAAATTGTAAAAGTTCATTGTTATTTAGATTTCAAAGTGAAGGGCATTTCAATCAAGTGATAATTCCAGATAA
AGCTAGTTTGCATTTCATTCTAGCAAATTTTCTTTGTTATTGATTTTGGCATAGTTAACAATCCAAAATAAATTCTAATTTGAAATAGATTAAAAAAAGGGTTTTACCTTTTAGTTTCAACCTATGAACTGCCTGATTTCTTTCCTACGTTAGTTTGTTTCAACCTATGAACTGCCTGATTTCTTTCCTAGGTTAGTTTG
TTTGTCTGGGTTGTTTATATCAATCTTTGTTTGCAGTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATAGAAAGAAACGAGATGGAATTGAATCAGgtagatgtacattttttcaaggtctttattaaactgttaatgtatagaaattagaattgaatcaggtagatgtacattgttct
acttattgcccagtcttctattgaaatagctctagctagttattaaaactaggtttagcttattgttgaaattatttagctgacattattaaaattcaaaatttatttggctcaaatttcactagatgactaagttatccttccagagattcatatcggccagCTATAACGAACGGTACCTCTGAAACCCTATTCCGAAG
TGCAAGGGCGAGAGAAAGCTTATGCGCCACTCTATATATAAGAAAGCCCTTAAAATTGAGCAGGCAATGTATATAAGAAACGCATGCCCTAAGATCCCTCTTATTGAATCTTGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGCTAAGACCGTAGTGCACAGAAAGCCTGGACTAGAAGGGGCTCGCTCTCTGC
ATCAGTTGGAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAAAAAGCCTCCTAGAAGGGGCTCGC

Intron 12 GTAGATGTAC ... ATCCTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	83.32	6	30	13
A2	93.72	2	30	6
A3	90.36	4	37.5	10
A4	87.98	3	22	6
A5	81.82	2	9	1
A6	70	12	28	12
A7	70.92	26	63.5	25
A8	67.04	26	53	16
A9	56.52	20	26	6
A10	63.58	22	38.5	10

>TRINITY_DN48047_c0_g1_i9 from sample A7 / Example spliced isoform

GAGACATTCTGGGCAGAACGGTTCCATTCTCAGTGCACAATTCAGAATTGGCCTTTGTTCAAGTGGCTGGAAGTTCGAACGCTGAGACATTCTGGGCAGAACGGTTCCATTCTCAGTGCACAATTCAGAATTGGCCTTTGTTCAAGTGGCTGGAAGTTTGTTGTCTTCGTACAAGCCCAATATGTATAAGAACGCCTGGA
ATTAGATCGTGGATGACTTCATCATTCTATAAAGGCATATGGTGCCATCATCTATGTGATGAGGTTTTATTTTTTATGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATAAGTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATAGAAAGAAACGAGATGGAATTGAATC
AGAGATTCATATCGGCCAGCTATAACGAACGGTACCTCTGAAACCCTATTCCGAAGTGCAAGGGCGAGAGAAAGCTTATGCGCCACTCTATATATAAGAAAGCCCTTAAAATTGAGCAGGCAATGTATATAAGAAACGCATGCCCTAAGATCCCTCTTATTGAATCTTGGATCTCTGCATCTTCCTCCACAACACCCATT
ATGAGCTAAGACCGTAGTGCACAGAAAGCCTGGACTAGAAGGGGCTCGCTCTCTGCATCAGTTGGAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAACCCTCCTAGAAGGGTTTAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAAATCTCTGCATCTTTCCTCGCTTCGGCTTGCATTTTCAGCCAC
AGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCTCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGGACGGTGCTTGAAAGAGTGGAGTTTGGAAGCCAGGGCACTTTCCAGAGATTTGTGAAGACCGTAGTGCAGAGAAAGCCTCCTAGAGAAAGGCACCTCCGATC
TCAAGTTGAGGGGATCTCCGTGTCTCCTTCCATTCCACCAACTTTCTGGTTGGTTTCGCTCTGGTTGGTTATAACGTGGATCTCTACAAAGTCTGCGTTATATAAAGGAGATTTACAGAGTTTCGTAAAAAGAAGTCACCGTCAAAATCCAGCCTGTACTTTTTCGGTATGGCAAGAGTGGAGTTTGGAAGCTTACTGCT
CAGAAAGCCTCCTAGAAGGGGCTCGCTTGGGGACATTTTGCTTTGCCTTCGCGCCGGCCACCTCCGATCTATCTATCATGGGCAATATACAGCAGCGGGGGATCCGTGTCTCCTTCGATTCC

>TRINITY_DN48047_c0_g1_i22 from sample A7 / Example unspliced isoform:

GAGACATTCTGGGCAGAACGGTTCCATTCTCAGTGCACAATTCAGAATTGGCCTTTGTTCAAGTGGCTGGAAGTTCGAACGCTGAGACATTCTGGGCAGAACGGTTCCATTCTCAGTGCACAATTCAGAATTGGCCTTTGTTCAAGTGGCTGGAAGTTTGTTGTCTTCGTACAAGCCCAATATGTATAAGAACGCCTGGA
ATTAGATCGTGGATGACTTCATCATTCTATAAAGGCATATGGTGCCATCATCTATGTGATGAGGTTTTATTTTTTATGGAATTAGATCGTGGATGACTTCATGATTCTATAAAGGCATAAGTGGTTGCGAAGATGAAATGACACCCCGCCGGAGAAAGAGAGACCATGAAGAATAGAAAGAAACGAGATGGAATTGAATC
AGgtagatgtacattttctcaaggtctttattaaattgttaatgtatagaaattagaattgaatcaggtagatgtacattgttctacttattgcccagtcttctattgaaatagctctagctagttattaaaactaggtttagcttattgttgaaattatttagctgacattattaaaattcaaaatttatttggctcaa
atttcactagatgactaagttatccttccagAGATTCATATCGGCCAGCTATAACGAACGGTACCTCTGAAACCCTATTCCGAAGTGCAAGGGCGAGAGAAAGCTTATGCGCCACTCTATATATAAGAAAGCCCTTAAAATTGAGCAGGCAATGTATATAAGAAACGCATGCCCTAAGATCCCTCTTATTGAATCTTGGA
TCTCTGCATCTTCCTCCACAACACCCATTATGAGCTAAGACCGTAGTGCACAGAAAGCCTGGACTAGAAGGGGCTCGCTCTCTGCATCAGTTGGAAAGCCAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAACCCTCCTAGAAGGGTTTAGGGATTTGTGAGCTAAGACCGTAGTGCACAGAAAATCTCTGCATCTT
TCCTCGCTTCGGCTTGCATTTTCAGCCACAGATTACCTGATGTAAGCCCCTCTGAAACTTTTGGTTTTTGATTCGCTCTTATTGAATCTCGGATCTCTGCATCTTCCTCCACAACACCCATTATGAGGACGGTGCTTGAAAGAGTGGAGTTTGGAAGCCAGGGCACTTTCCAGAGATTTGTGAAGACCGTAGTGCAGAGA
AAGCCTCCTAGAGAAAGGCACCTCCGATCTCAAGTTGAGGGGATCTCCGTGTCTCCTTCCATTCCACCAACTTTCTGGTTGGTTTCGCTCTGGTTGGTTATAACGTGGATCTCTACAAAGTCTGCGTTATATAAAGGAGATTTACAGAGTTTCGTAAAAAGAAGTCACCGTCAAAATCCAGCCTGTACTTTTTCGGTATG
GCAAGAGTGGAGTTTGGAAGCTTACTGCTCAGAAAGCCTCCTAGAAGGGGCTCGCTTGGGGACATTTTGCTTTGCCTTCGCGCCGGCCACCTCCGATCTATCTATCATGGGCAATATACAGCAGCGGGGGATCCGTGTCTCCTTCGATTCC

Intron 13 GTATGTTTGT ... TTCGTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.46	32	21	19
A2	54.95	25	28	33
A3	40.54	22	21	9
A4	46.24	25	21	22
A5	39.39	10	13	0
A6	25.93	30	11	10
A7	15.53	68	19	6
A8	13.33	65	12	8
A9	20	42	12	9
A10	12.42	67	11	8

>TRINITY_DN45556_c1_g1_i20 from sample A6 / Example spliced isoform

GTTGTGCAAAAAGCTAGAGTGCAGTTTGAAATTCAAACAGTTAGGTTAAGGAAAACAGACTTGGCCGAGAAGATATTAGGGTCTCCGTGAATGCAGGCCAGGGGAGTGCTACCTTACATAGAACAGACTTGGCCGATGGGGCCTGTTTAAAGATCTGATGGAGAGGGCCGAGAAGAAGATCGCCTCTGCTATATCTGCCC
GAATGCGCCAGTGGCCTTGGCGCGTTCGAATTCCAACCATATGGAGGGACTGCTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTG
AACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCATCGGCAGAGAAGAGGGATTGAATTCATATCAAATTTCCTTTGCATTATGTATAGGAGTTGAATCCTGAGTTATATTTGGTTTCCAGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGGGTTTCTTTTCCCTTGGTCGG
TGTATGGTTATGACGAATACTCTCTCACATGCATATGATTTTGGGAGAGCTCTGCTGGACTTTGGTTTCAGTCTTCAACTATGGATGAACCCTAACCCGTTTCGGAGCTACATGATCTTTTAAAACCATGGATGTCCGGGTTGCCGAGGACATGGTATCCTTTTAACAATTGCCGCAGAGATAGAATGCCAGAAATGGGC
GAGTAAAGGCATAAATATAGTACAAAG

>TRINITY_DN45556_c1_g1_i22 from sample A6 / Example unspliced isoform:

CTTGATGAATCTGAAGTATCTGAAGAATCAAATAAGATAACTTCTCTTCCGCCATAGATCGCCTACATGAGATCTGATTTAACCGTCCTGAAGCCGAATGACGTGGGTGACTCCCAAGTACGTCGGTGACTAGTAATCGAAGTGGCTGAACGGTGCGAGGAATTCAAATATTTAAGAACAAGTAGTTGGTGAATGCCCCA
TCGGCAGAGAAGAGGgtatgtttgtttctcttctggttttcaatgtggtaaaaaatggtgttcaacttctgagatttctgtagtgaactcgctgttgtttgttcgatttctgtgttaaaaaatggtgttcgatttctgatttctgtagtgatttgatgttcattcctttgtatttgaatggataacaatattaccaaatt
tctgggattaatcagagcctcaactctgtaaattaaaaaaatatatatccagattatatttctaggtttagaattgtatttgttttacaagtgtatgaccaaacccttatgaagggttgttcgcttcgtttcagGATTGAATTCATATCAAATTTCCTTTGCATTATGTATAGAAGTTGAATCCTGAGTTATATTTGGTT
TCCAGTTACCTCGATGCTTCCTGAGTTAAACTTCCTGCACTATCAGGTTTTTGCGCTTGAGCTGCCCATTCGTGTTCT