NA ----> NA

Intron 1 GTAAGAGATA ... TCTTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.43	150.9	9	17.7
A2	14.65	171.7	8	23.6
A3	10.92	224.9	0.8	24.3
A4	13.02	161.1	0.8	20.3
A5	9.09	135.4	0.8	11.5
A6	11.62	2249.7	7.5	226.8
A7	13.8	1178	9	161.8
A8	14.27	1065.5	7.8	150.8
A9	8.44	1143.4	3.5	76.3
A10	15.01	860.5	1.5	134.2

>TRINITY_DN38745_c0_g1_i2 from sample A6 / Example spliced isoform

CAAGCAACAATCTAGTTCAGAGGTAGGCCCTTTCTTTCTCGACTGCGCTGCCATTATCTTCTCTGTATATGATTAGAGCAACCGTTTCTGTGCAGAGCATTCAATTTATCATGTTTGCATAAACTAATGTTGCAGATTGTTTTGTAAAATATAGTTATGGTTTTTTTGTTTCAAGTTAATGTTTTACCCTGTTGTCCTTT
GCTTATGATTTCTTTCTTTGTTCTTTGACAGGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGGATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGTAAGCGATACTCCTTTCAAACTGAAATCATTCCGAGTTGGGAGAAAAAGATTCAAGGA
GGGCCAAGGTTGTATCTGCTTGCCGAATGGCACGTTTATCTCGGGGTTCTCTTTTGTGCGCAAGAGTCCCTTTCCATGCTTATAAAAATGAAATTTGCAGTGCTAGGGTTACCAATCCTTTTCTGGGTGTAGTGTAAAGGGTATAATTTCTTTCTTTTCTTTTGTGAAGAATGTCCTCAACAGGGTTCGACCCTGGTACA
AACTCTGAATTCGACCTGTTGTTTACAAATTTCTTTTCAGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGG
ATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAATCATAGTTTTCTTTCTT
CTAATTAGTGTTTCATAGTTTTAAAATCGT

>TRINITY_DN38745_c0_g1_i6 from sample A6 / Example unspliced isoform:

CAAGCAACAATCTAGTTCAGAGGTAGGCCCTTTCTTTCTCGACTGCGCTGCCATTATCTTCTCTGTATATGATTAGAGCAACCGTTTCTGTGCAGAGCATTCAATTTATCATGTTTGCATAAACTAATGTTGCAGATTGTTTTGTAAAATATAGTTATGGTTTTTTTGTTTCAAGTTAATGTTTTACCCTGTTGTCCTTT
GCTTATGATTTCTTTCTTTGTTCTTTGACAGGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGgtaagagataggccctttctttcttgactgcgctgcaattatctttcctgtatatgatgagagcaaccgtttctgtgcagagcattcaatttatcctgtttgcataaactaatg
ttgcaaattgttttgtaaaatatagttatggttttttgtttcaagttaatgttttgctctgttgtcctttgcttatgatttctttgacagGCTCCAGGATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGTAAGCGATACTCCTTTCAAACTGAAATCATTCCGAGTTGGGAGAAAAAGATTCA
AGGAGGGCCAAGGTTGTATCTGCTTGCCGAATGGCACGTTTATCTCGGGGTTCTCTTTTGTGCGCAAGAGTCCCTTTCCATGCTTATAAAAATGAAATTTGCAGTGCTAGGGTTACCAATCCTTTTCTGGGTGTAGTGTAAAGGGTATAATTTCTTTCTTTTCTTTTGTGAAGAATGTCCTCAACAGGGTTCGACCCTGG
TACAAACTCTGAATTCGACCTGTTGTTTACAAATTTCTTTTCAGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGG
TTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAATCATAGTTTTCTT
TCTTCTAATTAGTGTTTCATAGTTTTAAAATCGT

Intron 2 GTAAGCGATA ... TTCTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.22	149.9	102.8	69.8
A2	37.78	161.9	116	83
A3	38.26	226.4	159.1	125
A4	36.96	158.9	110	79
A5	30.3	140.9	80.6	44
A6	31.86	3166.5	1343.4	1645
A7	38.52	1416.7	866.4	923.2
A8	38.84	1466.9	739	1134
A9	32.28	1615	625	928
A10	37.69	974.5	650	539

>TRINITY_DN109293_c0_g1_i5 from sample A1 / Example spliced isoform

GTTAACCTCAAGAGGCCGCCCGTAAGATCAGAGGCAGGGACGACAGCATTACCACATTGGCTTTCTTTTGCAGGTTTTCAGATACGAAGTATACAGGCAAAGATTTGGAAAGCTGCAAGCAACAATCTAGTTCAGAGGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGG
ATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATT
TGGTTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAAT

>TRINITY_DN109293_c0_g1_i4 from sample A1 / Example unspliced isoform:

GTTAACCTCAAGAGGCCGCCCGTAAGATCAGAGGCAGGGACGACAGCATTACCACATTGGCTTTCTTTTGCAGGTTTTCAGATACGAAGTATACAGGCAAAGATTTGGAAAGCTGCAAGCAACAATCTAGTTCAGAGGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGG
ATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGgtaagcgatactcctttcaaactgaaatcattccgagttaggagaaaaagattcaaggagggccaaggttgtatctgcttgccgaatggcacgtttatctcggggttctcttttgtgcgcaagagtccctttccatgcttataaaaatgaaat
ttgcagtgctagggttaccaatccttttctgggtgtagtgtaaagggtataatttctttcttttcttttgtgaagaatgtcctcaacagggttcgaccctggtacaaactctgaattcgacctgttgtttacaaatttcttttcagGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCA
ATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTC
ACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAAT

Intron 3 GTAGGCCCTT ... TCTTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.14	171	16	45
A2	9.27	230	15	32
A3	14.73	231.5	9	71
A4	11.81	190.5	11	40
A5	13.53	147	6	40
A6	7.21	2608.5	106	299
A7	9.1	1309	55	207
A8	8.38	1203.5	66	154
A9	7.97	1430.5	81	167
A10	10.4	952	54	167

>TRINITY_DN32689_c0_g1_i2 from sample A9 / Example spliced isoform

TTTGCATACCGGTCAAGGGTGACGTTTACATTTTGAGATTACCTGTCAAATACGTTCTGAATTGCTAAATACGTTCTGTGCAACGTCAAATTCCAGGCCTGGACTCGAAGTGATAGCCGTCATAAAAGTTATTACGCCGATAGGCGTTGATATCTTGGATTTCTTGGAGAACTAAAGATAGATTTGCTAGTTCGAAGGTA
AGGACGCCAAGTGTGTGATCCAGATCTTTTTGATGGTCATCGTTTTTTTGTTAGCCTTAACTAGTAATTAACTGTGGCATAATGGAGAATGTTTCGTAGTTTTTACGTGGATTTAGGAATGCCTTGTGGTAGTTACGTGTGAGATCAAGCGTTGAGTTGTGATAACCGCAATTGTGATAACCGTTTTACCACACGGCATC
CGATCGAGGATCACCTTAACCACGCGGCAGATCGAGGATTAATGAGGATGGCAGTTAACCTCAAGAGGCCGCCCGTAAGATCAGAGGCAGGGACGACAGCATTACCACATTGGCTTTCTTTTGCAGGTTTTCAGATACGAAGTATACAGGCAAAGATTTGGAAAGCTGCAAGCAACAATCTAGTTCAGAGGCCACAGGAT
GAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGGATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAG
TTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGT
AATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAATCATAGTTTTC

>TRINITY_DN32689_c0_g1_i4 from sample A9 / Example unspliced isoform:

TTTGCATACCGGTCAAGGGTGACGTTTACATTTTGAGATTACCTGTCAAATACGTTCTGAATTGCTAAATACGTTCTGTGCAACGTCAAATTCCAGGCCTGGACTCGAAGTGATAGCCGTCATAAAAGTTATTACGCCGATAGGCGTTGATATCTTGGATTTCTTGGAGAACTAAAGATAGATTTGCTAGTTCGAAGGTA
AGGACGCCAAGTGTGTGATCCAGATCTTTTTGATGGTCATCGTTTTTTTGTTAGCCTTAACTAGTAATTAACTGTGGCATAATGGAGAATGTTTCGTAGTTTTTACGTGGATTTAGGAATGCCTTGTGGTAGTTACGTGTGAGATCAAGCGTTGAGTTGTGATAACCGCAATTGTGATAACCGTTTTACCACACGGCATC
CGATCGAGGATCACCTTAACCACGCGGCAGATCGAGGATTAATGAGGATGGCAGTTAACCTCAAGAGGCCGCCCGTAAGATCAGAGGCAGGGACGACAGCATTACCACATTGGCTTTCTTTTGCAGGTTTTCAGATACGAAGTATACAGGCAAAGATTTGGAAAGCTGCAAGCAACAATCTAGTTCAGAGgtaggccctt
tctttctcgactgcgctgccattatcttctctgtatatgattagagcaaccgtttctgtgcagagcattcaatttatcatgtttgcataaactaatgttgcagattgttttgtaaaatatagttatggtttttttgtttcaagttaatgttttaccctgttgtcctttgcttatgatttctttctttgttctttgacagg
ccacaggatgaggtctgagctactcaaggagtgactgaggggccatgcaaaacggtaagagataggccctttctttcttgactgcgctgcaattatctttcctgtatatgatgagagcaaccgtttctgtgcagagcattcaatttatcatgtttgcataaactaatgttgcagattgttttgtaaaatatagttatggt
ttttttgtttcaagttaatgttttaccctgttgtcctttgcttatgatttctttctttgttctttgacagGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGGATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGTAAGCGATACTCCTTTCAA
ACTGAAATCATTCCGAGTTGGGAGAAAAAGATTCAAGGAGGGCCAAGGTTGTATCTGCTTGCCGAATGGCACGTTTATCTCGGGGTTCTCTTTTGTGCGCAAGAGTCCCTTTCCATGCTTATAAAAATGAAATTTGCAGTGCTAGGGTTACCAATCCTTTTCTGGGTGTAGTGTAAAGGGTATAATTTCTTTCTTTTCTT
TTGTGAAGAATGTCCTCAACAGGGTTCGACCCTGGTACAAACTCTGAATTCGACCTGTTGTTTACAAATTTCTTTTCAGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTT
TATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAA
ACGATAATTCTATAAATATCAATCATAGTTTTC

Intron 4 GTTTGCATAA ... TCTTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.92	38	21.5	30.5
A2	36.95	30	16	20
A3	26.2	71	0	36
A4	35.04	32	5	22
A5	26.92	38	6	20.5
A6	26.7	267	9	149.5
A7	29.84	188	18.5	110.5
A8	28.78	144	5.5	81.5
A9	26.29	161	8	84.5
A10	24.99	171	5.5	84

>TRINITY_DN38745_c0_g1_i3 from sample A6 / Example spliced isoform

CAAGCAACAATCTAGTTCAGAGGTAGGCCCTTTCTTTCTCGACTGCGCTGCCATTATCTTCTCTGTATATGATTAGAGCAACCGTTTCTGTGCAGAGCATTCAATTTATCATGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGGATGCGGTCTGAGCTACTCAAGGAGT
GGCTGCGGGGCCATGCAAAACGGTAAGCGATACTCCTTTCAAACTGAAATCATTCCGAGTTGGGAGAAAAAGATTCAAGGAGGGCCAAGGTTGTATCTGCTTGCCGAATGGCACGTTTATCTCGGGGTTCTCTTTTGTGCGCAAGAGTCCCTTTCCATGCTTATAAAAATGAAATTTGCAGTGCTAGGGTTACCAATCCT
TTTCTGGGTGTAGTGTAAAGGGTATAATTTCTTTCTTTTCTTTTGTGAAGAATGTCCTCAACAGGGTTCGACCCTGGTACAAACTCTGAATTCGACCTGTTGTTTACAAATTTCTTTTCAGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCAT
TGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATT
GTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAATCATAGTTTTCTTTCTTCTAATTAGTGTTTCATAGTTTTAAAATCGT

>TRINITY_DN38745_c0_g1_i2 from sample A6 / Example unspliced isoform:

CAAGCAACAATCTAGTTCAGAGGTAGGCCCTTTCTTTCTCGACTGCGCTGCCATTATCTTCTCTGTATATGATTAGAGCAACCGTTTCTGTGCAGAGCATTCAATTTATCATgtttgcataaactaatgttgcagattgttttgtaaaatatagttatggtttttttgtttcaagttaatgttttaccctgttgtccttt
gcttatgatttctttctttgttctttgacagGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGGATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGTAAGCGATACTCCTTTCAAACTGAAATCATTCCGAGTTGGGAGAAAAAGATTCAAGGA
GGGCCAAGGTTGTATCTGCTTGCCGAATGGCACGTTTATCTCGGGGTTCTCTTTTGTGCGCAAGAGTCCCTTTCCATGCTTATAAAAATGAAATTTGCAGTGCTAGGGTTACCAATCCTTTTCTGGGTGTAGTGTAAAGGGTATAATTTCTTTCTTTTCTTTTGTGAAGAATGTCCTCAACAGGGTTCGACCCTGGTACA
AACTCTGAATTCGACCTGTTGTTTACAAATTTCTTTTCAGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCAATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGG
ATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTCACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAATCATAGTTTTCTTTCTT
CTAATTAGTGTTTCATAGTTTTAAAATCGT

Intron 5 GTAGGCCCTT ... TCTTTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.38	171	9.3	29.9
A2	7.62	230	9	19.5
A3	11.95	229.8	2.8	35.6
A4	9.59	190.5	4	21.5
A5	11.06	146.5	2.8	20.5
A6	5.15	2596	35.3	149
A7	7.32	1302.3	18.8	109.5
A8	5.99	1195.5	24	80.4
A9	5.33	1419	28	84.3
A10	7.54	947	21.3	83.9

>TRINITY_DN109293_c0_g1_i4 from sample A1 / Example spliced isoform

GTTAACCTCAAGAGGCCGCCCGTAAGATCAGAGGCAGGGACGACAGCATTACCACATTGGCTTTCTTTTGCAGGTTTTCAGATACGAAGTATACAGGCAAAGATTTGGAAAGCTGCAAGCAACAATCTAGTTCAGAGGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAACGGCTCCAGG
ATGCGGTCTGAGCTACTCAAGGAGTGGCTGCGGGGCCATGCAAAACGGTAAGCGATACTCCTTTCAAACTGAAATCATTCCGAGTTAGGAGAAAAAGATTCAAGGAGGGCCAAGGTTGTATCTGCTTGCCGAATGGCACGTTTATCTCGGGGTTCTCTTTTGTGCGCAAGAGTCCCTTTCCATGCTTATAAAAATGAAAT
TTGCAGTGCTAGGGTTACCAATCCTTTTCTGGGTGTAGTGTAAAGGGTATAATTTCTTTCTTTTCTTTTGTGAAGAATGTCCTCAACAGGGTTCGACCCTGGTACAAACTCTGAATTCGACCTGTTGTTTACAAATTTCTTTTCAGGGCGGTTGAGCCCAATATAACTATTCAGACTTTAGGCGGATCCTAAGTTTGTCA
ATTCTGAAACTGGTCTGTTCGGCATTGTTTCTATGGAAGCTCTAAGTTTAAACTGGCACCTTTTTTTTATGCTCAGAACACATCCTTCATTTGTAAATTTGGTTGGATAGTTTTATCTTTTGTATTGTTCCAAATCACCTGTGTGCGAGCATGTATTTTATTCATCTGTTCAATTTCAAAGCTAGCATAGGACTCTCTTC
ACAAATGAAAACTATTGGATCTATTGTTTAATGCGACTTCATTGGTAATTTTAATTTGATAAGGAAAACGATAATTCTATAAATATCAAT

>TRINITY_DN109293_c0_g1_i1 from sample A1 / Example unspliced isoform:

GTTAACCTCAAGAGGCCGCCCGTAAGATCAGAGGCAGGGACGACAGCATTACCACATTGGCTTTCTTTTGCAGGTTTTCAGATACGAAGTATACAGGCAAAGATTTGGAAAGCTGCAAGCAACAATCTAGTTCAGAGgtaggccctttctttctcgactgcgctgccattatcttctctgtatatgattagagcaaccgt
ttctgtgcagagcattcaatttatcatgtttgcataaactaatgttgcagattgttttgtaaaatatagttatggtttttttgtttcaagttaatgttttaccctgttgtcctttgcttatgatttctttctttgttctttgacagGCCACAGGATGAGGTCTGAGCTACTCAAGGAGTGACTGAGGGGCCATGCAAAAC
GGTAAGAGATAGGCCCTTTCTTTCTTGACTGCGCTGCAATTATCTTTCC