NA ----> NA

Intron 1 GTATGGTTTC ... CCTGTAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	35	29
A2	100	0	32	15
A3	95.45	1	30	12
A4	87.1	4	25	29
A5	100	0	8	7
A6	85.12	9	61	42
A7	84.11	12	81	46
A8	91.21	8	99	67
A9	91.61	6	73	58
A10	90.24	6	53	58

>TRINITY_DN47153_c0_g1_i2 from sample A10 / Example spliced isoform

CGGAAGAGGAGGAGATCATTCATGCCAACTGGACGAGGAGGAGATCAAGAAGCTGTGGAGCTGTGGATATCATCAGACTCCCAGGTATACAGTAATCCACGGTTCTATAGTATATTCACCAGAAAGTCGCTCCCAGTTTTGATCAGGAGATGGATCATGTTATAGTGTTTGAAACATTCACAAAATTGGCGTTGGAGTAT
ATCTGCAACAGTTGAGTCTACGCTTGGTTCTACAGAATTTAAAAGCACAATATTAAAGT

>TRINITY_DN47153_c0_g1_i1 from sample A10 / Example unspliced isoform:

CGGAAGAGGAGGAGATCATTCATGCCAACTGGACGAGGAGGAGATCAAGAAGCTGTGGAGCTGTGGATATCATCAGgtatggtttcgtgatctctgactttgcctatactgtaattgaaagaaaggaaagaactcagaatatcaatctagacttatggttagaaaagacatattgcgaattatttagttagatttgatgg
attattttcgctgtagcctctgtttttcatcgaaatttaatgtctattaaagaagttgacaacgaatatctgctgcctctcatttttcattttgaccttttttttcatggattacatttttttcatgtgtttccagtttctgaaaagcttccgaaccccagaaacctgaaaaacacttatagaatgaggggttgaactga
aaaacacttatagaatgctagaatctgctcattctatgaaaaacagtttctagtcattgtgaattatcattgaaagacctgtaacagACTCCCAGGTATACAGTAATCCACGGTTCTATAGTATATTCACCAGAAAGTCGCTCCCAGTTTTGATCAGGAGATGGATCATGTTATAGTGTTTGAAACATTCACAAGTAAGT
ATTCAATTGAATCTGATGCCTAATTTGCCGAAAAATAATAGCAAGCATTGTGTTGTTTAAAATTCCTTGCACTATCGATTCAGATTAGATTCTATTAGCCATTTTCAACAATCTGGGAATCAACCTGTTTTGGACGAACCTTCATTTTACCTTCTATATGTGTATTAAATCTAGGGTGCTTGATATTTAATAAATTTAGG
TTATAATGAAATGAAAATCTTTCATTTCTATTCATATAAAAACCCTAAATTGCAGAATTGGCGTTGGAGTATATCTGCAACAGTTGAGTCTACGCTTGGTTCTACAGAATTTAAAAGCACAATATTAAAGT

Intron 2 GTATGGTTTC ... GTGTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	93.65	2	35	24
A2	77.53	10	32	37
A3	84.62	4	30	14
A4	95.65	1	25	19
A5	66.67	4	8	8
A6	76.24	12	61	16
A7	68.35	25	81	27
A8	71.12	27	99	34
A9	68.57	22	73	23
A10	53.16	37	53	31

>TRINITY_DN47153_c0_g1_i3 from sample A10 / Example spliced isoform

CGGAAGAGGAGGAGATCATTCATGCCAACTGGACGAGGAGGAGATCAAGAAGCTGTGGAGCTGTGGATATCATCAGTTTCTGAAAAGCTTCCGAACCCCAGAAACCTGAAAAACACTTATAGAATGAGGGGTTGAACTGAAAAACACTTATAGAATGCTAGAATCTGCTCATTCTATGAAAAACAGTTTCTAGTCATTGT
GAATTATCATTGAAAGACCTGTAACAGACTCCCAGGTATACAGTAATCCACGGTTCTATAGTATATTCACCAGAAAGTCGCTCCCAGTTTTGATCAGGAGATGGATCATGTTATAGTGTTTGAAACATTCACAAGTAAGTATTCAATTGAATCTGATGCCTAATTTGCCGAAAAATAATAGCAAGCATTGTGTTGTTTAA
AATTCCTTGCACTATCGATTCAGATTAGATTCTATTAGCCATTTTCAACAATCTGGGAATCAACCTGTTTTGGACGAACCTTCATTTTACCTTCTATATGTGTATTAAATCTAGGGTGCTTGATATTTAATAAATTTAGGTTATAATGAAATGAAAATCTTTCATTTCTATTCATATAAAAACCCTAAATTGCAGAATTG
GCGTTGGAGTATATCTGCAACAGTTGAGTCTACGCTTGGTTCTACAGAATTTAAAAGCACAATATTAAAGT

>TRINITY_DN47153_c0_g1_i1 from sample A10 / Example unspliced isoform:

CGGAAGAGGAGGAGATCATTCATGCCAACTGGACGAGGAGGAGATCAAGAAGCTGTGGAGCTGTGGATATCATCAGgtatggtttcgtgatctctgactttgcctatactgtaattgaaagaaaggaaagaactcagaatatcaatctagacttatggttagaaaagacatattgcgaattatttagttagatttgatgg
attattttcgctgtagcctctgtttttcatcgaaatttaatgtctattaaagaagttgacaacgaatatctgctgcctctcatttttcattttgaccttttttttcatggattacatttttttcatgtgtttccagTTTCTGAAAAGCTTCCGAACCCCAGAAACCTGAAAAACACTTATAGAATGAGGGGTTGAACTGA
AAAACACTTATAGAATGCTAGAATCTGCTCATTCTATGAAAAACAGTTTCTAGTCATTGTGAATTATCATTGAAAGACCTGTAACAGACTCCCAGGTATACAGTAATCCACGGTTCTATAGTATATTCACCAGAAAGTCGCTCCCAGTTTTGATCAGGAGATGGATCATGTTATAGTGTTTGAAACATTCACAAGTAAGT
ATTCAATTGAATCTGATGCCTAATTTGCCGAAAAATAATAGCAAGCATTGTGTTGTTTAAAATTCCTTGCACTATCGATTCAGATTAGATTCTATTAGCCATTTTCAACAATCTGGGAATCAACCTGTTTTGGACGAACCTTCATTTTACCTTCTATATGTGTATTAAATCTAGGGTGCTTGATATTTAATAAATTTAGG
TTATAATGAAATGAAAATCTTTCATTTCTATTCATATAAAAACCCTAAATTGCAGAATTGGCGTTGGAGTATATCTGCAACAGTTGAGTCTACGCTTGGTTCTACAGAATTTAAAAGCACAATATTAAAGT

Intron 3 GTAAGTATTC ... TAAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.49	2	17	26
A2	100	0	13	18
A3	89.47	1	10	7
A4	77.78	4	17	11
A5	100	0	4	2
A6	85.71	2	18	6
A7	47.54	16	23	6
A8	65.79	13	42	8
A9	76.27	7	42	3
A10	76.19	5	30	2

>TRINITY_DN47153_c0_g1_i2 from sample A10 / Example spliced isoform

CGGAAGAGGAGGAGATCATTCATGCCAACTGGACGAGGAGGAGATCAAGAAGCTGTGGAGCTGTGGATATCATCAGACTCCCAGGTATACAGTAATCCACGGTTCTATAGTATATTCACCAGAAAGTCGCTCCCAGTTTTGATCAGGAGATGGATCATGTTATAGTGTTTGAAACATTCACAAAATTGGCGTTGGAGTAT
ATCTGCAACAGTTGAGTCTACGCTTGGTTCTACAGAATTTAAAAGCACAATATTAAAGT

>TRINITY_DN47153_c0_g1_i3 from sample A10 / Example unspliced isoform:

CGGAAGAGGAGGAGATCATTCATGCCAACTGGACGAGGAGGAGATCAAGAAGCTGTGGAGCTGTGGATATCATCAGTTTCTGAAAAGCTTCCGAACCCCAGAAACCTGAAAAACACTTATAGAATGAGGGGTTGAACTGAAAAACACTTATAGAATGCTAGAATCTGCTCATTCTATGAAAAACAGTTTCTAGTCATTGT
GAATTATCATTGAAAGACCTGTAACAGACTCCCAGGTATACAGTAATCCACGGTTCTATAGTATATTCACCAGAAAGTCGCTCCCAGTTTTGATCAGGAGATGGATCATGTTATAGTGTTTGAAACATTCACAAgtaagtattcaattgaatctgatgcctaatttgccgaaaaataatagcaagcattgtgttgtttaa
aattccttgcactatcgattcagattagattctattagccattttcaacaatctgggaatcaacctgttttggacgaaccttcattttaccttctatatgtgtattaaatctagggtgcttgatatttaataaatttaggttataatgaaatgaaaatctttcatttctattcatataaaaaccctaaattgcagAATTG
GCGTTGGAGTATATCTGCAACAGTTGAGTCTACGCTTGGTTCTACAGAATTTAAAAGCACAATATTAAAGT