sp|Q8RWH3|YAK1_ARATH ----> Dual specificity protein kinase YAK1 homolog


Intron 1 GTAATTGGTT ... TTTCTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A124.02371.971.1164
A226.74269.463.7133
A324.0132161.9141
A425.1126963.4117
A523.28114.922.747
A617.652109
A726.3211121.358
A825.2514.16.63
A927.166016.728
A1026.3112.925.655



>TRINITY_DN110092_c0_g1_i6 from sample A2 / Example spliced isoform
TTCAAATCAACAGCTCCAATCCATTCAATTTTCCACAAACCCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGG
AATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGATCTGTAAACAGGGGAAAT
TCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGA
TGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCA
CTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAAT
CAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGA
AGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAAC
TTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGT
TCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGATAACATACCTTT
CAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGA
GATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTG
GACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGG
CTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGG
AGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAAC
TTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAG
GGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAA
GGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAA
TCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTT
TGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTG
AGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAG
TTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGA
AGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATA
AAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAG
GAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACAGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCT
GAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAAC
ACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGG
CGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTAATTG
AGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCA


>TRINITY_DN110092_c0_g1_i1 from sample A2 / Example unspliced isoform:
TTCAAATCAACAGCTCCAATCCATTCAATTTTCCACAAACCCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGG
AATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGATCTGTAAACAGGGGAAAT
TCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGgtaattggtt
tggcaaattgaatccttggtcctggagcttttgtaggtcagtctgtttgtagtatgttcattctagactctatatgtggaatgcagtgcattttggttgaatatttgtgatctttctggtatgtttcttgcagTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGT
ACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGC
ATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTC
AAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAG
TGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCA
AAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAA
GGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGATAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAA
TACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGC
AAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGA
TCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGA
TGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAAT
ATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTA
ATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGA
GATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGA
GCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGC
AATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCC
AAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATG
TTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTAC
TTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAA
TATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATG
TTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACAGACTT
CCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGC
ATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTC
AACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTG
TTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTAATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCA



Intron 2 GCGGTTTCTC ... ATTATATGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.0551195115
A218.0948993123
A317.33551100131
A419.9241292113
A518.512514668
A618.032574
A716.481092122
A812.52817
A918.371001629
A1021.312053972



>TRINITY_DN42138_c0_g1_i3 from sample A9 / Example spliced isoform
CTATTCCCAGCTATTAATTTCAAATCAACAGCTCCAATCCATTCAATTTTCCACAAACCCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAA
ATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGA
TCTGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTC
ATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGTTTCTCCATCCCTTT
TGTTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCCTAAA
ATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGA
TTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAA
CTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTG
CATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCT
CTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAGCAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACT
CAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTT
GATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCA
AGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAG
ACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATG
GATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGA
CTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATG
AGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCA
AAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGC
ATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGA
AGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGAT
GAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAAT
TACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATAT
TAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATG
CTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACAGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTC
ATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTAC
TGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGT
CACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGT
TGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGCATCTTAAACTGTTACAATTAGTCTGCATTACATAAATGAAAAGATGGATTCCATGTTCTCTACTACGTTTAAAATAATCAGCAACGATCCGAACATATA
TATTGTGATGTTCTGTTTTATGCCTTTGAATGTTGCGGC


>TRINITY_DN42138_c0_g1_i7 from sample A9 / Example unspliced isoform:
CTATTCCCAGCTATTAATTTCAAATCAACAGCTCCAATCCATTCAATTTTCCACAAACCCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAA
ATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGA
TCTgcggtttctccttccgatctcgccgttccgggtttcgtaattcgcggcattggattcgagttcgatgttcgcgggctcatttcatttattatggaatgctaacccctgtaagacatccggtgggttgtaattcgtggcagtgttcgcgagttcaattcccgcggagtcatttcatgtattatcgaatgctaatgcaa
accccagtaagacctccattcagctgtaagttcgattcccgcaagcgtgctatagaatatgagccctaataagtccttcgattaccgtgcctgtgaagtccggaagtcttgaccggaaactccagtctcccgatggtttgcacttggctccgaaactgtcaccctgcagcttgcgagtccgattaaaattgtgggcgatt
atatgagGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAAT
CTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGTTTCTCCATCC
CTTTTGTTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCC
TAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGC
ACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCA
GAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTT
GTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGC
CTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAGCAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAA
GACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGG
GCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTG
GCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAG
CAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTT
GATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAG
CTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAG
AATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGG
ATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTA
CTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACAC
AGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCT
TGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGT
CAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTT
ATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCA
CATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACAGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTA
TCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTT
CTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTG
TGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTG
AAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGCATCTTAAACTGTTACAATTAGTCTGCATTACATAAATGAAAAGATGGATTCCATGTTCTCTACTACGTTTAAAATAATCAGCAACGATCCGAACA
TATATATTGTGATGTTCTGTTTTATGCCTTTGAATGTTGCGGC



Intron 3 GTCAGTCTGT ... TTTCTTGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A144.692039164
A243.3217419133
A342.3419213141
A443.3315322117
A547.4752247
A647.371009
A743.9474758
A827.27813
A945.1634128
A104470955



>TRINITY_DN110092_c0_g1_i4 from sample A2 / Example spliced isoform
TTCAAATCAACAGCTCCAATCCATTCAATTTTCCACAAACCCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGG
AATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGATCTGTAAACAGGGGAAAT
TCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGGTAATTGGTT
TGGCAAATTGAATCCTTGGCCCTGGAGCTTTTGTAGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGT
GAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCT
ACTTTCTCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTG
TGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGAC
AAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCC
AGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAG
AGAATGTTTCAACCTTTCACGAGCCTCTCTCAGATAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGAT
TTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAA
GAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTG
AGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACA
GGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTT
AATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTG
GTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTG
TCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCC
TTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATA
ATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAG
ACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAA
CAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGT
ATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCT
CGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATAC
ATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACAGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTT
GGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATT
CTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGC
GAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCT
ATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTAATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCA


>TRINITY_DN110092_c0_g1_i1 from sample A2 / Example unspliced isoform:
TTCAAATCAACAGCTCCAATCCATTCAATTTTCCACAAACCCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGG
AATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGATCTGTAAACAGGGGAAAT
TCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGGTAATTGGTT
TGGCAAATTGAATCCTTGGTCCTGGAGCTTTTGTAGgtcagtctgtttgtagtatgttcattctagactctatatgtggaatgcagtgcattttggttgaatatttgtgatctttctggtatgtttcttgcagTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGT
ACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGC
ATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTC
AAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAG
TGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCA
AAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAA
GGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGATAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAA
TACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGC
AAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGA
TCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGA
TGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAAT
ATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTA
ATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGA
GATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGA
GCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGC
AATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCC
AAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATG
TTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTAC
TTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAA
TATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATG
TTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACAGACTT
CCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGC
ATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTC
AACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTG
TTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTAATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCA



Intron 4 GTAAGTTCAA ... ATTATATGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A119.42510.1130.9115
A222.47489160.6123
A321.05550.1162.4131
A423.03412133.6113
A521.2225167.268
A620.632594
A719.46108.630.422
A813.852827
A923.081003129
A1024.62204.661.772



>TRINITY_DN123938_c6_g1_i11 from sample A1 / Example spliced isoform
CCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATT
TCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGA
TTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGAC
ACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAA
TGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCCGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAG
ATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAA
AATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAG
GGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATG
CAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGG
AAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTC
GCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGG
TTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGAT
TCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATA
CTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACAC
AAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAA
TACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAA
TAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGG
ATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAA
GAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCAT
TGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTC
TACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGT
GATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGA
GCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTT
CATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAA
TATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATT
GCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGA
GCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTA
ATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGC


>TRINITY_DN123938_c6_g1_i2 from sample A1 / Example unspliced isoform:
CCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATT
TCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTgtaagttcaaatcttcacacagtccttgaagcccagttcacgtagcctagggttt
ttttacccttaggtctttgaattttacactgttctcccacattgccgatccggcatttttttctcgacaatgtttacccgccaggcggtttctccttccgatctcgccgttccgggtttcgtaattcgcggcattggattcgagttcgatgttcgcgggctcatttcatttattatggaatgctaacccctgtaagacat
ccggtgggttgtaattcgtggcagtgttcgcgagttcaattcccgcggagtcatttcatgtattatcgaatgctaatgcaaaccccagtaagacctccattcagctgtaagttcgattcccgcaagcgtgctatagaatatgagccctaataagtccttcgattaccgtgcctgtgaagtctggaagtcttgaccggaaa
ctccagtctcccgatggtttgcacttggctccgaaactgtcaccctgcagcttgcgagtccgattaaaattgtgggcgattatatgagGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTG
GGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTG
CGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGA
AGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCCGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAG
GAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGC
AATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGT
TCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAG
ATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGAC
CAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGA
AAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTT
CAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGAC
GAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGA
GGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAAC
CACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGC
TCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAA
AAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGA
GGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAG
GCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTAT
ACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTT
TGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTG
ATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCG
AGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACC
TTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACT
AACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTG
ATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATAT
TTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGC



Intron 5 GTATAGCAGT ... AAAATATAAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A123.28369121103
A222.762468164
A324.222558380
A421.172296459
A524.88773318
A620.2949797156
A721.81882192300
A820.261000204304
A922.28731188231
A1022.13658159215



>TRINITY_DN44258_c0_g5_i7 from sample A8 / Example spliced isoform
CACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGC
AGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGT
TGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTT
GGTTAACCGTGAGGGTGCCAATGTTTCTCCATCCCTTTTGTTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAG
ACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGAC
AAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAA
TACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGGAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATG
CTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGAT
CATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGATAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAGCAAGCAGTGAATAG
AGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAAT
CAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAG
GGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGA
AAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATG
ACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTG
AGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAG
GCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTT
TTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAA
AACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGA
TGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCA
TAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGA
GGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATA
TGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAG
GGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTA
AAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGAT
GGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCA
AGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCG
AGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGCATCTTAAACTGTTACAATTAGTCCGCAC


>TRINITY_DN44258_c0_g5_i2 from sample A8 / Example unspliced isoform:
CACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGC
AGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGT
TGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTT
GGTTAACCGTGAGGGTGCCAATGTTTCTCCATCCCTTTTGTTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAG
ACAAGCTGCTACTTTCTCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGAC
AAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAA
TACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGGAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATG
CTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGAT
CATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGATAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAGCAAGCAGTGAATAG
AGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAAT
CAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAG
GGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGA
AAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATG
ACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTG
AGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAG
GCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTT
TTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAA
AACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGA
TGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCA
TAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGA
GGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATA
TGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAG
GGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGgtatagcagtctttatacaccatttgtgtatagcaattaaactgttaatttaaatgtgtagggaagttgatgattgtcatagattgcagtcattaatgacaaaatataagGAAACAAACAGGCTGGAGTACTTGATACCTAAAA
AGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTT
ACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAA
CAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGA
GGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTA
TTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGCATCTTAAACTGTTACAATTAGTCCGCAC



Intron 6 GTTGGTTTTG ... TGTTATGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A122.612195969
A222.511363940
A320.341415022
A422.521293540
A527.56461817
A620.221463638
A721.234280104
A820.093508294
A919.743075992
A1024.142425797



>TRINITY_DN123938_c6_g1_i2 from sample A1 / Example spliced isoform
CCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATT
TCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAGTTCAAATCTTCACACAGTCCTTGAAGCCCAGTTCACGTAGCCTAGGGTTT
TTTTACCCTTAGGTCTTTGAATTTTACACTGTTCTCCCACATTGCCGATCCGGCATTTTTTTCTCGACAATGTTTACCCGCCAGGCGGTTTCTCCTTCCGATCTCGCCGTTCCGGGTTTCGTAATTCGCGGCATTGGATTCGAGTTCGATGTTCGCGGGCTCATTTCATTTATTATGGAATGCTAACCCCTGTAAGACAT
CCGGTGGGTTGTAATTCGTGGCAGTGTTCGCGAGTTCAATTCCCGCGGAGTCATTTCATGTATTATCGAATGCTAATGCAAACCCCAGTAAGACCTCCATTCAGCTGTAAGTTCGATTCCCGCAAGCGTGCTATAGAATATGAGCCCTAATAAGTCCTTCGATTACCGTGCCTGTGAAGTCTGGAAGTCTTGACCGGAAA
CTCCAGTCTCCCGATGGTTTGCACTTGGCTCCGAAACTGTCACCCTGCAGCTTGCGAGTCCGATTAAAATTGTGGGCGATTATATGAGGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTG
GGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTG
CGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGA
AGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCCGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAG
GAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGC
AATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGT
TCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAG
ATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGAC
CAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGA
AAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTT
CAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGAC
GAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGA
GGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAAC
CACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGC
TCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAA
AAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGA
GGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAG
GCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTAT
ACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTT
TGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTG
ATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCG
AGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACC
TTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACT
AACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTG
ATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATAT
TTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGC


>TRINITY_DN123938_c6_g1_i3 from sample A1 / Example unspliced isoform:
CCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATT
TCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGA
TTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGAC
ACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAA
TGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCCGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAG
ATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAA
AATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAG
GGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATG
CAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGG
AAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTC
GCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGG
TTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGAT
TCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATA
CTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACAC
AAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAA
TACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAA
TAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGG
ATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAA
GAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCAT
TGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTC
TACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGgttggttttgcttaagaaggttttttcattatgattgcaattagaaatatttgtgtattttgg
gtttttattcacttgaattaagcctgtctacctcaatatagtcgataacctctttattacttgcataattttgtttgttatgcagTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGA
AGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTA
GCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTC
TTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCT
GTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCT
GTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTT
CATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGC



Intron 7 GTTTGGCTTT ... TTATGACTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.22351173
A220.12258265
A319.05323076
A420.13254164
A522.22112032
A629.7933014
A726.41209075
A823.7174023
A923.83163051
A1023.29247075



>TRINITY_DN102319_c0_g1_i7 from sample A4 / Example spliced isoform
AAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATTTCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCAT
GGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCGACGATCTGTAAGTTCAAATCTTCACACAGTCCTTGAAGCCCAGTTCACGTAGCCTAGGGTTTTTTTACCCTTAGGTCTTTGAATTTTACACTGTTCTCCCACATTGCCGATCCGGCATTTTTTTC
TCGACAATGTTTACCCGCCAGGCGGTTTCTCCTTCCGATCTCGCCGTTCCGGGTTTCGTAATTCGCGGCATTGGATTCGAGTTCGATGTTCGCGGGCTCATTTCATTTATTATGGAATGCTAACCCCTGTGAGACATCCGGTGGGTTGTAATTCGTGGCAGTGTTCGCGAGTTCAATTCCCGCGGAGTCATTTCATGTAT
TATCGAATGCTAATGCAAACCCCAGTAAGACCTCCATTCAGCTGTAAGTTCGATTCCCGCAAGCGTGCTATAGAATATGAGCCCTAATAAGTCCTTCGATTACCGTGCCTGTGAAGTCTGGAAGTCTTGACCGGAAACTCCAGTCTCCCGATGGTTTGCACTTGGCTCCGAAACTGTCACCCTGCAGCTTGCGAGTCCGA
TTAAAATTGTGGGCGATTATATGAGGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATG
GGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGT
GCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTC
TCTGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGA
CTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTT
TCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGGAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTC
TGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATG
TTTCAACCTTTCACGAGCCTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAGCAAGCAGTGAATAGAGGTAAAGATTTTGAC
AGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCC
AAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATG
CTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACAT
GGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGC
TCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAG
GGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAA
TATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAG
CACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCAC
CACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTC
AACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGA
TTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTA
CCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCT
TATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAA
GTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTAT
CGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCG
TTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAAT
GTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTT
GAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTG


>TRINITY_DN102319_c0_g1_i1 from sample A4 / Example unspliced isoform:
GCCAGGAAATTACATCTTCAGgtttggctttagtagtttgcttcaactaattcagcatttgcacaatgtgtagtggcaatggacaatttttttttttttgctttttgttcatgcattgctaatctcctttatacttatgggatttctatgtgtcccaagagcattttccccctgttatgttgcatttacctgccatattg
cagagaaaagccctttagaatcgtacatatcaaaacaattttgtattattgcctattgataacagccttctgctactgatctgcaaaggtgcccaatgtatcatacctgagcatggtgtttgaactgaattttctatttactgcctctgaacttaatgtttaatttgttatgactagGTGGGAAGCGTACAATGGGAAGC
AGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAAT
CGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTG
AGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGAT
GATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGA
CAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTC
TTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCT
GTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACG
ACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCA
TGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCAT
AAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCA
AACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAG
AGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGA
CCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTG
AGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAA
GAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATT
AGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTG



Intron 8 GTAATTGGTT ... GCTTTTGTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A129.89372.6114.6203
A233.33269.795.7174
A331.86321.6108.7192
A431.44269.193.9153
A527.63114.935.752
A620.7521110
A732.8611134.873.8
A841.4214.111.98
A931.0160.520.434
A1033.151134270



>TRINITY_DN123938_c6_g1_i2 from sample A1 / Example spliced isoform
CCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATT
TCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAGTTCAAATCTTCACACAGTCCTTGAAGCCCAGTTCACGTAGCCTAGGGTTT
TTTTACCCTTAGGTCTTTGAATTTTACACTGTTCTCCCACATTGCCGATCCGGCATTTTTTTCTCGACAATGTTTACCCGCCAGGCGGTTTCTCCTTCCGATCTCGCCGTTCCGGGTTTCGTAATTCGCGGCATTGGATTCGAGTTCGATGTTCGCGGGCTCATTTCATTTATTATGGAATGCTAACCCCTGTAAGACAT
CCGGTGGGTTGTAATTCGTGGCAGTGTTCGCGAGTTCAATTCCCGCGGAGTCATTTCATGTATTATCGAATGCTAATGCAAACCCCAGTAAGACCTCCATTCAGCTGTAAGTTCGATTCCCGCAAGCGTGCTATAGAATATGAGCCCTAATAAGTCCTTCGATTACCGTGCCTGTGAAGTCTGGAAGTCTTGACCGGAAA
CTCCAGTCTCCCGATGGTTTGCACTTGGCTCCGAAACTGTCACCCTGCAGCTTGCGAGTCCGATTAAAATTGTGGGCGATTATATGAGGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGATTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTG
GGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGTTATTACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTG
CGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATGGCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGA
AGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCCGGATGAGTTTTCTCCTAAAATTGGAAGAATGAATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAG
GAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAACAGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGC
AATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGTCCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGT
TCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAGGGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAG
ATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGAGAACATACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGAC
CAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGGAAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGA
AAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAAAAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTT
CAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGGTGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGAC
GAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTGGAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGA
GGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAGCTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAAC
CACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGAAGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGC
TCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAACAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAA
AAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCTGGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGA
GGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTCCAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAG
GCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTGGCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTAT
ACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTTCTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTT
TGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATGTCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTG
ATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTTATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCG
AGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGGAATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACC
TTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCTTCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACT
AACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGGGAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTG
ATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTCCCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATAT
TTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCTTTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGC


>TRINITY_DN123938_c6_g1_i9 from sample A1 / Example unspliced isoform:
CCTCACACTTCAAATACATCAGAAGGCACGTGGCAACACCTCAGCACTTAGCAAAGGGGTGAAAAAGAGGGGTCAGAGCGATGTCAGTGTGCCACCACCCTCTGCACAACCACAACAAAATAGAAATAAAAACAATAATGAAATAAGATAAATTAAATGGAATGGGAAGGGGGCAAAACACTCAAAAACATTTTTTCATT
TCCCCTTTACTATTTGTTGCCTGTTGGTGGTGGCACTGGCGCTCCGTGTGCACTGTCTCCCATGGAAGTACGACGAGGAAAATGAAGATTGGCAACCAGCTAGCAGCAGCAGGTGCCTCGGCACGATTCGAATCCGCCACGATCTGTAAACAGGGGAAATTCTATTGATGCACCTAGAACTATGTGAGATTTCTTCTGGA
TTTGAGGCACATTAATTATAAACACTCTTTAAAAGAGTGAAAACAATTATTAGGCTGGGGTAGCGAAAACAATTACTAGGCTGGGGTGCTGCAGTTCTAAGAGTTGATGCTGTGGTTATGGGGGCAGAACTTTCAAATCTTCATTCACTGgtaattggtttggcaaattgaatccttggccctggagcttttgtagTTAT
TACAGATGGTATCATAGCATTAGTTGCCTAAACTACAGATCAGGACACTGCAGAGGTGATTGTACCAATGGCAGACACTGCGAGCATTATTCTGGATTATTTGCGTAGGCATCATTTTACAAAGGCTGAAGCAGCTCTGCGAGGGGAGTTGGTTAACCGTGAGGGTGCCAATGGTTCTCCATCCCTTTTGCTCTTGGATG
GCTTCACTTCTCTATCCACAGAAGGAAATAGAGAAGAGAGCACCAATGAGGACAAGCAAGGGCATCAAATCCAGAGGGAAGAAACTGGATATGCTCAGAATGAAGATGAAAGAATGAAATGTCTGACTGAAGATATTGCCTTTGAGGAAGACAAGCTGCTACTTTCTCCGGATGAGTTTTCTCCTAAAATTGGAAGAATG
AATAATCAGTCATTAGGCCAGAGAAGTTTGCTAGACAGTGCAACAGATACAGAGCTTATAGTCAAGGAGGTGGATTGTCATCCCATTAAAAATGGACCCGCAGGAGACCACTCAAATGCAAGAAACAATCCAGTTATTAATGAAGGTGACAAGGCGGCTGTGTGGACTGCACAAAGGATAGTGCACGATTCATCTGGAAC
AGGTGAAGCTAGAAACATATCCCGGCAGGATTCTGCAAGGTCTAAAAATGTGAGTGGTTTAAGTGCTTTAGACAAATATGCATGGAGTTTGGGTGCTGGATGCAATGAGATGAAAGGATATGCAGAGGGAAACATTGATGGTACTGTTAATACTGCAGACAAGTTTTCTGAGTCACAAAAACCAGAAACTTCTTTTGGGT
CCAAACTTCAAGTTCACGAGTCTCCAAATATAAGTAACGTTACAAGGGAGACAAGGAAGTCCAAAACAACAGCTGGAGGGTCACTGTATGAAGAAGTGTGTGTTCAGTATCCATCAAATGCAACTTCACGACTTGGTGGAAAAGGAAATGCTAGGATGCCAGGTTCTGCCGGAGACCAGAGGTTGTTGCATGGAAGGGAG
GGCAGTTCTACAAAAGAGGAAGAGAAAGTCTCATGGTCAGAAAATGCAAATGTTCTGGATGAAGGAATCGTAGACTCTAGAGACCATTCTAGGTTGACCCAAGATTCTGAAGTTTTTATTCAGTTCCCTTCAGGAGGTGTAAGGGCGGATCATTATAAAGAGAATGTTTCAACCTTTCACGAGCCTCTCTCAGAGAACAT
ACCTTTCAAACCAGAGGAGCGTAGGTTGATCTCCATTGACCCATGGAAAGAAAGGCCAATCAATACGGCCTTTCCGTTTCCCTTAATTGATAATTGTTCTGACCAGGGAAAAGTATTGCAAAACGTGAACATAAAACAAGCAGTGAATAGAGGTAAAGATTTTGACAGACAAGGTGCATGCAAAGACTCAGGGACCATGG
AAGAGAGATCAGACAAGGAGCATATATCTTTACACATGCTAACCTCGCAAGGAAGTATAGAGCAAGAGAATTTGGAGAGTCTAGAGATAGTGGCTACTCCAGAAAAGACTTCAGAGGAGCTCCCAAGGTTGCCACCTGTCAGGCTTAAATCAGAGGAGAAGAACCCAAACTTTTCACAGGAAGGGCTTGATAGGGGAGAA
AAATTGGACCGAGCAGGATCTAGCGCGAAGGCATTGAACATGGAGGTTTCATTTCTATTGGGATCATTTTTGGATGTGCCTGTTGGCCAGGAAATTACATCTTCAGGTGGGAAGCGTACAATGGGAAGCAGTAGACTATCTGTAAGCCAGGGCATTGCTGAGGATGCTTCAGAACTTGTTTCTGGCCAAGCTACTGTTGG
TGATGGCTTGAGTGAGTCTGTTGATTATCCAAACGAGTATTGGGATTCTGATGAATATGAAGATGATGATGACCTGGGATACATCAGGCAGCCCATAGAAGACGAGACTTGGTTTTTAGCTCATGAAATCGATTATCCTAGTGACAATGAAAAAGGAACAGGACATGGCAGTATTCCAGATCAGCAAGACAGAGATGGTG
GAAAGGAGGAGGATGATGACCAATCCTTTGTAGAAGAAGATTCATACTTTTCAGGTGAACAATATTTTCCATCAAAAAATATTGAACAAATAGCTGGTTCAGAGGACCCAGCAGGACAATCTACAACTGAGATGTATGGAAGAAGCGATGACAATGACTTAATAGCTCAGTATGATGGACAGTTGATGGATGAAGAGGAG
CTGAACTTGATGAGAGCAGAACCTGTGTGGCAAGGTTTTGTTACACAAAACAATGAGCTTGTAATGTTAGGCAGTGAGAGGGTTATGAGTGATCTAGAGCAACCACGACAGGATGACTTTGGCATTGATGATGATCAACATGGGTCGGTGAGGTCTATTGGTGTAGGGATTCATAGCGATGTAGCTGACTTTGGCAGTGA
AGTGAGGGAAAGCTTGGTTGGAGGGAGCAGTGAAGGAGATGTTGAATACTTCCCTGATCGGGAGATTAGTATCTCTGGTTCTAAGCATGTTAAATATGATCGCTCAAGGAGAACACATGAAATGCTGGACAAAACTAAAGAGTTACCAAGGCAAGATTTGTCTAAATATGATGCTCTAGCTGAGAATGAGGACAGCCTAA
CAGGAAGGAGTTATGATTCTTGGCAGGATCGGAGAAAATCTGGGAATAGTCAAGCAGGGCAGAGCATCTCTGAAGATCAAGACGCTGTGAAAGATCTTGTCAAAAGAAGGGATGCTGACAGTGGTCGTCTTGACTTTGGAGGGTTCTCTTTTCCATCTCCTTCTAGCACTGGTGATATGGTAGGATCAAAGGCAGAATCT
GGAAAATCTCTGTGGTCAACCAAGGGGAATACTACAGCTTGTGAGGATACAGATGACTATGGCAATGACATAATTGGGCCTGATGATGTTTTGGCATCATGGAGGCGCAAGAGCAATGATTCTTCACCTGTTATAAGCTCAAGGGATGAAAACCTGAATAATGCACCACGGTCTTCACACTCTACTGCATCTACATATTC
CAACTTTGAACTTGCTGAAAGAGAATTGAGGAAAAGGGATACAGAAGAGAATTTGAATGACCCAAGGGAAGATGAAGAAGTAGGGGTAATAGAGGATGAAGAGGCTGCAGCTGTACAAGAACAAGTACGACAGATAAGAGCACAAGAAGATGAGTTTGAGACATTCAACCTCAAGATTGTACACAGGAAGAATAGGACTG
GCTTTGAGGAAGACAAAAATTTTCATGTTGTTCTTAACTCAGTCATTGCTGGCCGCTACCATGTTACTGAGTATCTTGGTTCGGCAGCATTTAGCAAAGCTATACAAGCCCATGATTTGCACACGGGCATGGATGTTTGCATGAAGATCATAAAGAACAACAAGGATTTTTTTGACCAAAGTCTTGATGAAATAAAGCTT
CTTAAGTTTGTAAACAAGCATGATCCAGCTGACAAGTACCACATTCTACGCTTGTACGATTACTTTTACTATCGTGAGCATCTATTCATCGTGTGTGAACTTTTGAAAGCAAACTTGTATGAATTCCATAAATTTAATAGAGAGTCTGGAGGAGAGGTGTATTTTACCATGCCAAGATTACAGTCAATTACACTTCAATG
TCTGGAAGCATTACATTTTTTGCATGGCCTTGGTCTCATTCACTGTGATCTGAAGCCAGAGAATATCCTGGTTAAAAGCTACAGTAGGTGTGAGGTGAAGGTGATAGATCTTGGTAGCAGCTGCTTTCAAACGGATCATCTTTGCTCATATGTGCAGTCTCGCTCTTATCGTGCACCGGAGGTTATATTAGGTCTCCCTT
ATGATAAAAAGATTGACATCTGGTCACTTGGATGCATTCTTGCTGAGCTTTGTTCTGGAAATGTTCTCTTTCAGAATGATTCCCCTGCAACCTTGCTAGCTCGAGTAATTGGAATAATTGGACCAATAGAGCAGGAGATGCTCGTGAAAGGGCGTGATACATACAAGTATTTTACGAAAAATCACATGCTCTATGAACGG
AATCAGGAAACAAACAGGCTGGAGTACTTGATACCTAAAAAGACTTCATTGAGACACCGACTTCCAATGGGGGATCAAGGATTTATTGATTTTGTTTCTTACCTTCTTGAAATCAATCCTGCTAAACGACCTTCTGCTACTGAGGCACTAAAACACCCTTGGCTATCGTACCCCTATGAACCTATCTCATCTTGATCCCT
TCAGCTGAGTATTGGAAATGTCTTATTGAGGGAAGCGCTTACAAAATATGACATTCTTGTAGCATATACCACAACATTTGTGAAATTCTTTATATGCTGTACTAACATCAATAGAAGCTGCCCTCCTTGAGAGCAAATATAAGGATGGATGGCACTAATTCTGTCGTTATGACAAATGTTAGTTCTACTGACATTGTTGG
GAGAACACCTAAATGCTCCAGTTGCTCTTTTTCTTGCTAACAGATTGCTGTAATAGTATTTTCAACCTTTGAGGCATCGATCATCTATCATGTGTCCTGTGTGATCAAAGATATCTGTGCACATTGTAAGAAGTCACATCCTTGGCAGCAAGTCTAAAGCGAAAATGTTAACCAACCCAGCTTGTGGTCACTCATGTGTC
CCAAGGCGGTGGTTTATTTTGCTTGATGTGTATTGAATGAGGTTGAGCTGTTCCTTGGTATTGTTCTGTAACGAGTCTTCTAGCTGGAGAATGTATTTCATATTTACTGCGGTATGAAGTTTCCATATTAGTGGTATTCTTATTATTGCGAGTGAATTCTATGCTTGAAGATGACATGTTATTGAAGTTGTAAATGATCT
TTATTGAGAGTTACTTTGATTAAAAACCTTTGGACACGTATTCTTAATAACGGCTAAAAGTTGATCTTAAACTGTTACAAATAGTCTGC