NA ----> NA

Intron 1 GCAGTTTCAA ... TAATGATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80	7	28	28
A2	80.77	5	26	16
A3	83.53	7	41	30
A4	79.71	7	36	19
A5	62.16	7	14	9
A6	74.43	39	108	119
A7	77.89	43	169	134
A8	76.74	50	166	164
A9	75.53	46	145	139
A10	77.85	35	143	103

>TRINITY_DN43267_c11_g3_i1 from sample A10 / Example spliced isoform

TAACCTAATGGAAGCGACATGGGGCAACGGAGCCGAACAATTCACAAAGATAATATAAAACTTAGCTTTGATATTACGCACCGAATTCATACATAATTCAACGTATGTTTCACATTTATATTTATAATTGTAATATATGTCTTCTGAGTTAGACTGGGGCGATTGACAATAAGAATAAGAACGAGGAGGGGTGAGAACCG
GGCAGGGGAATTGGCAAGAAAGAGACGCAATCATAATTACAGAAATGCCAGATTTTGCAGTTCCCATAGAGCCCTGCTCGGAGCGTCCTCTGTTCGGAGGCGCCATTGCCTGTACATTCCCCCAGCGTTTTCAGGATCTGAGCAACATAAGAGAGGTTCCGGATCATCAGGAGGCATTTGCAGATCCGAATCGTGACGAA
AGCCTGATTGTTGAGCTACTGGACCTAAAACATGATGTTTCAGATAACCAAAGTGCATTATGGTTTCTTCAGGACCTTGCTAATGAACAGGATTCTGCTGGAGACCTTGTGGTAGAAAGTTTGAGTGCTGTTGCTCCATCTGATGCCCCTTTCCTTGATCCAGCAACTGTTATCACGACTGTAGTTGGGAAAATGTGTAC
TGAGTGAGAGTGCTGCAACCGTTGGACCGGGAGTTCCAGTTTCTGCAGCAGCAGCTGGTTGCATGCCTGTTGTGGAGGCCTTCAAGCTGTGTCTTGCGACATTCAAAATTCACAATTGGAGACTTTTTGGACCTTGAGTTGGTTGCTCTAGGATTTTCAATTTTTGGTTTCTGACGTCTTTATTTTCTGCAGTTGTCTTT
AATCTCTTTGAGGTTTAATTCGAACAGACAACAAACAACAGGTTGGTATGGTGTTTGTCTTGAACATTATTATATATATATATATATATATAT

>TRINITY_DN43267_c11_g3_i3 from sample A10 / Example unspliced isoform:

TAACCTAATGGAAGCGACATGGGGCAACGGAGCCGAACAATTCACAAAGATAATATAAAACTTAGCTTTGATATTACGCACCGAATTCATACATAATTCAACGTATGTTTCACATTTATATTTATAATTGTAATATATGTCTTCTGAGTTAGACTGGGGCGATTGACAATAAGAATAAGAACGAGGAGGGGTGAGAACCG
GGCAGGGGAATTGGCAAGAAAGAGACGCAATCATAATTACAGAAATGCCAGATTTTGCAGTTCCCATAGAGCCCTGCTCGGAGCGTCCTCTGTTCGGAGGCGCCATTGCCTGTACATTCCCCCAGCGTTTTCAGGATCTGAGCAACATAAGAGAGGTTCCGGATCATCAGGAGGCATTTGCAGATCCGAATCGTGACGAA
AGCCTGATTGTTGAGCTACTGGACCTAAAACATGATGTTTCAGATAACCAAAGTGCATTATGGTTTCTTCAGGACCTTGCTAATGAACAGGATTCTGCTGGAGACCTTGTGGTAGAAAGTTTGAGTGCTGTTGCTCCATCTGATGCCCCTTTCCTTGATCCAGCAACTGTTATCACGACTGTAGTTGGGAAAATGgcagt
ttcaaaaggcaggcaagggagtgaggcgcgtaatatagtaagggtttatttggcaaacatccgtctgaaaggagttggcactgatttacttattactgcatatgaacctttaatgatcagTGTACTGAGTGAGAGTGCTGCAACCGTTGGACCGGGAGTTCCAGTTTCTGCAGCAGCAGCTGGTTGCATGCCTGTTGTGG
AGGCCTTCAAGCTGTGTCTTGCGACATTCAAAATTCACAATTGGAGACTTTTTGGACCTTGAGTTGGTTGCTCTAGGATTTTCAATTTTTGGTTTCTGACGTCTTTATTTTCTGCAGTTGTCTTTAATCTCTTTGAGGTTTAATTCGAACAGACAACAAACAACAGGTTGGTATGGTGTTTGTCTTGAACATTATTATAT
ATATATATATATATATAT

Intron 2 GTGCCACATT ... ATTTGTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.54	46	19.7	6
A2	4.65	41	17.9	2
A3	9.8	46	16.4	5
A4	12.5	35	12.9	5
A5	24.14	22	4.3	7
A6	15.44	126	45	23
A7	13.94	142	33	23
A8	11.19	127	32	16
A9	9.18	178	44.9	18
A10	16.94	103	23.7	21

>TRINITY_DN41174_c2_g1_i3 from sample A6 / Example spliced isoform

GGGCAACGGAGCCGAACAATTCACAAAGATAATATAAAACTTAGCTTTGATATTACGCACCGAATTCATACATAATTCAACGTATGTTTCACATTTATATTTATAATTGTAATATATGTCTTCTGAGTTAGACTGGGGCGATTGACAATAAGAATAAGAACGAGGAGGGGTGAGAACCGGGCAGGGGAATTGGCAAGAAA
GAGACGCAATCATAATTACAGAAATGCCAGATTTTGCAGTTCCCATAGAGCCCTGCTCGGAGCGTCCTCTGTTCGGAGGCGCCATTGCCTGTACATTCCCCCAGCGTTTTCAGGATCTGAGCAACATAAGAGAGGTTCCGGATCATCAGGAGGCATTTGCAGATCCGAATCGTGACGAAAGCCTGATTGTTGAGCTACTG
GACCTAAAACATGATGTTTCAGATAACCAAAGTGCATTATGGTTTCTTCAGGACCTTGCTAATGAACAGGATTCTGCTGGAGACCTTGTGGTAGAAAGTTTGAGTGCTGTTGCTCCATCTGATGCCCCTTTCCTTGATCCAGCAACTGTTATCACGACTGTAGTTGGGAAAATGGCAGTTTCAAAAGGCAGGCAAGGGAG
TGAGGCGCGTAATATAGTAAGGGTTTATTTGGCAAACATCCGTCTGAAAGGAGTTGGCACTGATTTACTTATTACTGCATATGAACCTTTAATGATCAGGTTGGTCTGGGAAGGAAATCTTGTTTCCTTGCAAACCTAGATGAGTTTCCCTATTTTGTAATTTTCTAAAACATGCAGTAAATAATAAATATTGAAAGTCT
TTTCTTTTTAACGTGTGTTTAAAAAAATAAAAATATTTT

>TRINITY_DN41174_c2_g1_i1 from sample A6 / Example unspliced isoform:

GGGCAACGGAGCCGAACAATTCACAAAGATAATATAAAACTTAGCTTTGATATTACGCACCGAATTCATACATAATTCAACGTATGTTTCACATTTATATTTATAATTGTAATATATGTCTTCTGAGTTAGACTGGGGCGATTGACAATAAGAATAAGAACGAGGAGGGGTGAGAACCGGGCAGGGGAATTGGCAAGAAA
GAGACGCAATCATAATTACAGAAATGCCAGATTTTGCAGTTCCCATAGAGCCCTGCTCGGAGCGTCCTCTGTTCGGAGGCGCCATTGCCTGTACATTCCCCCAGCGTTTTCAGGATCTGAGCAACATAAGAGAGGTTCCGGATCATCAGgtgccacattctgttgactgcttatgaatgagagatcctgtctgatagtta
atgtgtacaaaacgtaaattatatgtccaaatttttaatgaagtcggtttgttctggatattggtggttcgctattttaaactagttgtcaaaattttaatttttgcataagtggtttgttctagaggctgctggggcactttgtagggtagaggtagttaactttccacattatcaaagtacacggacttaagccatat
acatggcagtggctaatgctattggtgcatctactttgaaaggtagcaccgtatgaagctcaaaattactactcacatgtaagttcatctattaaaatattctgtttgatttgtgaagGAGGCATTTGCAGATCCGAATCGTGACGAAAGCCTGATTGTTGAGCTACTGGACCTAAAACATGATGTTTCAGATAACCAAA
GTGCATTATGGTTTCTTCAGGACCTTGCTAATGAACAGGATTCTGCTGGAGACCTTGTGGTAGAAAGTTTGAGTGCTGTTGCTCCATCTGATGCCCCTTTCCTTGATCCAGCAACTGTTATCACGACTGTAGTTGGGAAAATGGCAGTTTCAAAAGGCAGGCAAGGGAGTGAGGCGCGTAATATAGTAAGGGTTTATTTG
GCAAACATCCGTCTGAAAGGAGTTGGCACTGATTTACTTATTACTGCATATGAACCTTTAATGATCAGTGTACTGAGTGAGAGTGCTGCAACCGTTGGACCGGGAGTTCCAGTTTCTGCAGCAGCAGCTGGTTGCATGCCTGTTGTGGAGGCCTTCAAGCTGTGTCTTGCGACATTCAAAATTCACAATTGGAGACTTTT
TGGACCTTGAGTTGGTTGCTCTAGGATTTTCAATTTTTGGTTTCTGACGTCTTTATTTTCTGCAGTTGTCTTTAATCTCTTTGAGGTTTAATTCGAACAGACAACAAACAACAGGTTGGTATGGTGTTTGTCTTGAACATTATTATATATATATATATATATATATTGCATCATTCACTTTATTTATTTAATGGCAGCCG
TTGCATAATGGTTAAGAATCAATATTTGTGAACACTCAATTTATTGGGGTTTATGACAAATTGGACTCTCTATGAGTTTGTTGCTAAAGCTGATCAAGTATATTATAAGTTCAATGATTGCCAGGAAGCCATGACAAATAATGGTCGTGATGATATTTGGCTTAGTATGCCTGTGTGAACCACTATGTTTTTACCCTGAA
CAATCTAGTATTAGATTTGTCCCAGCCTCTATTATAAAGGATGACATGAGA

Intron 3 GTATGTTGAT ... TTTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.33	43	10	24
A2	23.68	29	15	3
A3	21.67	47	20	6
A4	14.67	32	6	5
A5	22.22	14	6	2
A6	24.14	132	48	36
A7	21.02	186	60	39
A8	22.81	181	68	39
A9	20.94	151	45	35
A10	21.56	151	59	24

>TRINITY_DN109437_c0_g1_i1 from sample A1 / Example spliced isoform

CCTGGGGCGATTGACAATAAGAATAAGAACGAGGAGGGGTGAGAACCGGGCAGGGGAATTGGCAAGAAAGAGACGCAATCATAATTACAGAAATGCCAGATTTTGCAGTTCCCATAGAGCCCTGCTCGGAGCGTCCTCTGTTCGGAGGCGCCATTGCCTGTACATTCCCCCAGCGTTTTCAGGATCTGAGCAACATAAGA
GAGGTTCCGGATCATCAGGAGGCATTTGCAGATCCGAATCGTGACGAAAGCCTGATTGTTGAGCTACTGGACCTAAAACATGATGTTTCAGATAACCAAAGTGCATTATGGTTTCTTCAGGACCTTGCTAATGAACAGGATTCTGCTGGAGACCTTGTGGTAGAAAGTTTGAGTGCTGTTGCTCCATCTGATGCCCCTTT
CCTTGATCCAGCAACTGTTATCACGACTGTAGTTGGGAAAATGGCAGTTTCAAAAGGCAGGCAAGGGAGTGAGGCGCGTAATATAGTAAGGGTTTATTTGGCAAACATCCGTCTGAAAGGAGTTGGCACTGATTTACTTATTACTGCATATGAACCTTTAATGATCAGTGTACTGAGTGAGAGTGCTGCAACCGTTGGAC
CGGGAGTTCCAGTTTCTGCAGCAGCAGCTGGTTGCATGCCTGTTGTGGAGGCCTTCAAGCTGTGTCTTGCGACATTCAAAATTCACAATTGGAGACTTTTTGGACCTTGAGTTGGTTGCTCTAGGATTTTCAATTTTTGGTTTCTGACGTCTTTATTTTCT

>TRINITY_DN109437_c0_g1_i4 from sample A1 / Example unspliced isoform:

TATTGGTGCATCTACTTTGAAAGGTAGCACCGTATGAAGCTCAAAATTACTACTCACATGTAAGTTCATCTATTAAAATATTCTGTTTGATTTGTGAAGGAGGCATTTGCAGATCCGAATCGTGACGAAAGCCTGATTGTTGAGCTACTGGACCTAAAACATGATGTTTCAGATAACCAAAGTGCATTATGGTTTCTTCA
GGACCTTGCTAATGAACAGGATTCTGCTGGAGACCTTGTGGTAGAAAGTTTGAGTGCTGTTGCTCCATCTGATGCCCCTTTCCTTGATCCAGCAACTGTTATCACGACTGTAGTTGGGAAAATGGCAGTTTCAAAAGGCAGGCAAGGGAGTGAGGCGCGTAATATAGTAAGGgtatgttgatccttcttttttatctgtt
ccattttaaggaatcactggggcattttctacaactcgttttgggttttttttatgcagGTTTATTTGGCAAACATCCGTCTGAAAGGAGTTGGCACTGATTTACTTATTACTGCATATGAACCTTTAATGATCAGTGTACTGAGTGAGAGTGCTGCAACCGTTGGACCGGGAGTTCCAGTTTCTGCAGCAGCAGCTGGT
TGCATGCCTGTTGTGGAGGCCTTCAAGCTGTGTCTTGCGACATTCAAAATTCACAATTGGAGACTTTTTGGACCTTGAGTTGGTTGCTCTAGGATTTTCAATTTTTGGTTTCTGACGTCTTTATTTTCT