sp|P16128|Y4849_ARATH ----> Uncharacterized protein At4g18490

Intron 1 GTGGGTAGCT ... GAAAAAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.76	16	21	0
A2	58.18	69	96	1
A3	59.63	44	65	0
A4	60	32	48	1
A5	52.63	18	20	0
A6	59.36	280	409	10
A7	59.39	361	528	9
A8	59.25	260	378	16
A9	58.77	461	657	10
A10	58.28	300	419	9

>TRINITY_DN43930_c0_g1_i10 from sample A8 / Example spliced isoform

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACGTAAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCAGCAGTAGTGGCCATTTTTATAAA
AATGGACAAACTACGACTTTAGATTCCAGCAACACATTTACCTGTAACATCACATTTCCAGCCGTACATTATTTTTTGTTACAGCGTCACATTTGGTGCCACATAAAGGGATCTTTGATGTTCAATGCATAGTTGAGAGGCACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCAGAATTTTGT
CCACAAAGACTTTGGTTTTGGAAAGCTTGAAAAAAAAGGTTACAACTTTAGATTCCAACAACACACTATACTATACCATTTGTGGTTACAGAAGTTATAGTGGCCATTTTTATAAAAATTGATAAGTGTTATTTTTTAAATAATAGGTATTATTATTATTTGAGTTGGAAAATTCAATGTCTGTTTTATTGTTAAAATGA
TTGGTGATTTTGTTATTTTCGGGTTCCCTTTAGC

>TRINITY_DN43930_c0_g1_i12 from sample A8 / Example unspliced isoform:

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACGTAAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGgtgggtagctgttagaatttacaatgtctcaaaaaagaaaaatccaagtcaccattggagaaatgtatggatggaaaaaagaaca
agaatccaaaattcaaaatgattcaaacactagtgaagaagcttataatactgaattggagcagcaattggatgatatttttggaagattcgctgatgaagatgtagatgaagaaagagatgaagttgaagaagaggttgataatgaaaagaaaatgaaagtgcaatataaaggaaattctgtatgggatggttagataa
gtttgattggctgaattatgtgaggcaggatgatatttagtatatatgaaatgcagggattgtgagaagtataggatgaatggtccatggggtttagggaatggttgtacgactttgcaacatgatgctcttgttactcatgaaaaatcattagtacataaagatgctaaagcaagatggattaatgcattggagagaaa
gatgaagccaattttagatcatatcaaacaagtggaagatgctaataagcaaagagttattgctacaatgagaatatcatattttgtatgccaagaagatttatccttatcaaagtacgaaaaactatgcaaatttctcatggatgcgaaaacaccaaatatgccaaagcctcaagagtattctttatacacaaatcgta
aatctgcaaatgatttcatatattccatatcaagacaccttgaagaggttcagataaagaatatgttggaaagtcctttcttctcattaatggttgatgaaagcacagatcgaagtttagagcagcaccttgttgtttatgcaacttatttagatgcaaagggtcttgggccacctatttcacaatttatgaagctgaaa
aatgtaatggatggaaaagggaaaacaatatatgatagtgtcaactagttaataaatgttagaggtcttcaatataaagatttgatagttgtttctacagatggggcttcagcaatgattggacatgaaaatggatttgtttcatttttgaaaaaagacactccaaatcttattacagcccattttgtagctcatcgcga
gtctctagcagcaacagatgcttctaaaaaaattcctgaactattatatgttgagaaaattgcaaacaaagtgtattcgtggattcaaaactcaccaaagagaagtaatgaacttaatgatttgcttaaagtcatgcagattgatgttctacaaatacacttagtgagatggttatctagggtggaagttattataaggc
ttgtgaagcttatgctagcgattttaacattgtgaaaaaatgaaagaaaaacttcatcatggtatgataaaacatgaatttattccattcagttttgtttgcatatgcttgctgatgtgttgggtgaattgaataagctgaacgagacttttcaagaagaaaatgcagacattacaactattgggttggctcttgaagta
actataagtacattgtctagatcgtttttgagaaaagaaacatttgcagaaggtacaacttatctttcaaaatttctttatgattcacaatatggatatattgaaattaaagatgaagggttagtcatagatagacatgagttacaatatatctctattccaactgcggatattgattatagtgattggaaacaaactat
gaaacttcatattgaaggaacacaagagagttgtcagcaactcacagaaggctatattgagtctctcatagactctttaaatggaagatttccatatcttcatctttttaatgcagcaaaattgttcatccattgttatgatccagaagaacgtcgtagccatgagaaaaattcagagcgttggctttctaaactttttc
agcatttgcagcataccttaccaaatggtgtcagaaatgtgcatttatttgatgaaaaagcttgcaaaagagaactgcactcttttgttgattcattaaatttggcttgtgagggtttctctatgaaagaagcatggaaggcctttcgtaatacaaaacaatggcacacaaattatcctcaaatgatgaaattatggcaa
gctgtactcactattcctacaagtacagtggattgtgaaaggggattttctaagcagaatataattaaggacattaggaaatcaaagctaggattggatacactagatgcactaatgagaatatttttgaatggattgaaattatcagctgttgattggaatgcagtgtatgagatatggagagatacaaagtcaagaag
agttcttgagctttgagagtagggaagtcttcctagtcctctcaagtgagttgtgtttagtttcaaaccaatgttttaagcaccgtgtgtatgtttttatattagatttaagcatttgtctctcatatcttatttttaatttaagaattgttcttgcaggattttttgaaaattttgaacttcatatggaatcacgaaat
ttacagcagtagtggccattttttaaaacaagattttttgaaaattttgaacttcatatggattcacgaaatttacagcattagtggccattttcttaaaaatggacaagttcacatttagtgccacatgaagggttctttgatgttccaagcatatttgagaaacacctctttttgtaaatctgagtccagccataact
caaaaccaaatcatcataattttgtccacaaagactttgattttggaaggcttgaaaaaaaagGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCAGCAGTAGTGGCCATTTTTATAAAAATGGACAAACTACGACTTTAGATTCCAGCAACACATTTACCTGTAACATCA
CATTTCCAGCCGTACATTATTTTTTGTTACAGCGTCACATTTGGTGCCACATAAAGGGATCTTTGATGTTCAATGCATAGTTGAGAGGCACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCAGAATTTTGTCCACAAAGACTTTGGTTTTGGAAAGCTTGAAAAAAAAGGTTACAACTTTAGA
TTCCAACAACACACTATACTATACCATTTGTGGTTACAGAAGTTATAGTGGCCATTTTTATAAAAATTGATAAGTGTTATTTTTTAAATAATAGGTATTATTATTATTTGAGTTGGAAAATTCAATGTCTGTTTTATTGTTAAAATGATTGGTGATTTTGTTATTTTCGGGTTCCCTTTAGC

Intron 2 GTAAGAAACA ... TTCAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.89	132.5	23.3	28.5
A2	12.24	300	41	40.8
A3	12.34	250.8	20.8	33
A4	10.4	190.3	19.3	21.3
A5	10.66	104.5	9.3	11.8
A6	12.97	1932.5	68.8	257.8
A7	11.92	2555.5	93	311
A8	12.38	1972.5	69.3	247
A9	10.97	2975.5	89.3	329.8
A10	10.94	2160.5	89.3	241.5

>TRINITY_DN125074_c7_g1_i4 from sample A1 / Example spliced isoform

ACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGAGGAAGCCATGCC
TTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGTGTATGAGATATGGAGAGATACAAAGTCAAGAAGAGTTCTTGAGCTTTGAGAGTAGGGAAGTCTTCCTAGTCCTCTCAAGATTTTTTGAAAATTTTGAACTTCATA
TGGAATCACGAAATTTACAGCAGTAGTGGCCATTTTTTAAAACAAGATTTTTTGAAATTTTGAACTTCATATGGATTCACGAAATTTACAGCATTAGTGGCCATTTTCTTAAAAATGGACAAGTTCACATTTAGTGCCACATGAAGGGTTCTTTGATGTTCCAAGCATATTTGAGAAACACCTCTTTTTGTAAATCTGAG
TCCAGCCATAACTCAAAACCAAATCATCATAATTTTGTCCACAAAGACTTTGATTTTGGAAGGCTTGAAAAAAAAGGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCT

>TRINITY_DN125074_c7_g1_i1 from sample A1 / Example unspliced isoform:

ACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGAGGAAGCCATGCC
TTCCATTCAAACAGCGCTTCCACCGGAGCTTGCCAACAATGTCAGAAACgtaagaaacactcacaagcattcatatccctgttcagataaaacatttacacaactaattcgccccttcaatgcagATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGCAAGGTAACACAGAGCTGGTGGTTGATATG
ATTCGCCAACAGTTCAAGAAAAATATGCGCGAGACAGATCCAGAAAAGATACAAAAGATGAAAGATGATGCTGCGAGAGGCCTTGTAAATCACATGCTTTATGAATCAGAAAAAATGTCGGGACGCAAGTTTTCTACTCCCTAGAGGAATTGGTCATTTTACAATACCATGGCTATCACATTGTAGCTGACATGCATCCA
AATTGTTGCAATGTTTTGTAAAACAGAATTGCAAGTTCAACTTTCTACCAAATAAAAACTATTAGTTGGAACTCATTTTCTCCTCGTGTTATTTACTGTTGCAGATACCAATTTTATGAAAACTTTATGGAGTCTTACCATTAAAATTCAATTGTAATAGAGTATTGTATTCCACAATTGTATGATCACTAGAAACAATG
ATCGTAATGAAAGTGTTTTGTTTAGAAAGCTAAAAAA

Intron 3 GTTCACATTT ... GAAAAAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.34	28	31.5	0
A2	53.44	103.5	114.5	1
A3	54.36	74	85	0
A4	53.23	57	62	1
A5	50.06	32.5	33.5	0
A6	52.89	499	546.5	10
A7	52.83	658.5	716	9
A8	53.17	473	519	16
A9	52.66	779.5	845	10
A10	52.14	537.5	573	9

>TRINITY_DN43930_c0_g1_i10 from sample A8 / Example spliced isoform

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACGTAAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCAGCAGTAGTGGCCATTTTTATAAA
AATGGACAAACTACGACTTTAGATTCCAGCAACACATTTACCTGTAACATCACATTTCCAGCCGTACATTATTTTTTGTTACAGCGTCACATTTGGTGCCACATAAAGGGATCTTTGATGTTCAATGCATAGTTGAGAGGCACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCAGAATTTTGT
CCACAAAGACTTTGGTTTTGGAAAGCTTGAAAAAAAAGGTTACAACTTTAGATTCCAACAACACACTATACTATACCATTTGTGGTTACAGAAGTTATAGTGGCCATTTTTATAAAAATTGATAAGTGTTATTTTTTAAATAATAGGTATTATTATTATTTGAGTTGGAAAATTCAATGTCTGTTTTATTGTTAAAATGA
TTGGTGATTTTGTTATTTTCGGGTTCCCTTTAGC

>TRINITY_DN43930_c0_g1_i5 from sample A8 / Example unspliced isoform:

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACGTAAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGgttcacatttagtgccacatgaagggttctttgatgttccaagcatatttgagaaacacctctttttgtaaatctgagtccagcc
ataactcaaaaccaaatcatcataattttgtccacaaagactttgattttggaaggcttgaaaaaaaagGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCAGCAGTAGTGGCCATTTTTATAAAAATGGACAAACTACGACTTTAGATTCCAGCAACACATTTACCTGTA
ACATCACATTTCCAGCCGTACATTATTTTTTGTTACAGCGTCACATTTGGTGCCACATAAAGGGATCTTTGATGTTCAATGCATAGTTGAGAGGCACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCAGAATTTTGTCCACAAAGACTTTGGTTTTGGAAAGCTTGAAAAAAAAGGTTACAAC
TTTAGATTCCAACAACACACTATACTATACCATTTGTGGTTACAGAAGTTATAGTGGCCATTTTTATAAAAATTGATAAGTGTTATTTTTTAAATAATAGGTATTATTATTATTTGAGTTGGAAAATTCAATGTCTGTTTTATTGTTAAAATGATTGGTGATTTTGTTATTTTCGGGTTCCCTTTAGC

Intron 4 GTAAGAAACA ... TTCAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.84	119.5	21	18
A2	9.43	277	38	18
A3	5.22	223.5	17	6
A4	6.58	176	17	7
A5	7.79	94	7	8
A6	2.53	1659.5	58	18
A7	2.87	2215	87	30
A8	2.38	1698	52	21
A9	2.3	2589	83	27
A10	3.14	1895.5	82	30

>TRINITY_DN93182_c0_g1_i2 from sample A4 / Example spliced isoform

TCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGAGGAAGCCATGCCTTCCA
TTCAAACAGCGCTTCCACCGGAGCTTGCCAACAATGTCAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGGTATGTATAGTACTTATTCTTTAAACCTTCTCCAGAACTAAATTTATTGCAATGCTCTGGTTACCTCGTCCACATTCTTTAACACCGTCTAAGCTGTATACCTTTTGGGTC
TCAACAGTTTACTGACGGAAACCATAAAACCCTAGAAAGCTCCTGCCTCTCAATTTCAATTTAGATTAGATGGACAGACGAACTGAATGTGTTTTAGGAATAAAAAACTCAATTAGAATAACATCAGTCTCTAATGAATGCGTTTGTTAGAACTTGTTAAATGTAATTACAGAAACCCAATACTCTAAAGAACAGGAAAA
AGCCG

>TRINITY_DN93182_c0_g1_i3 from sample A4 / Example unspliced isoform:

TCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGAGGAAGCCATGCCTTCCA
TTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACgtaagaaacactcacaagcatatcatatccctgttcagattaaacatttacactgaactaattcgccccttcaatgcagATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGGTATGTATAGTACTTATTCTTTAAACCTTCTC
CAGAACTAAATTTATTGCAATGCTCTGGTTACCTCGTCCACATTCTTTAACACCGTCTAAGCTGTATACCTTTTGGGTCTCAACAGTTTACTGACGGAAACCATAAAACCCTAGAAAGCTCCTGCCTCTCAATTTCAATTTAGATTAGATGGACAGACGAACTGAATGTGTTTTAGGAATAAAAAACTCAATTAGAATAA
CATCAGTCTCTAATGAATGCGTTTGTTAGAACTTGTTAAATGTAATTACAGAAACCCAATACTCTAAAGAACAGGAAAAAGCCG

Intron 5 GTAAGAAACA ... TTCAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	19.48	115.1	21.9	25
A2	11.88	277.2	39.2	35.9
A3	12.67	222.4	18.5	29.2
A4	9.94	175	17.9	18.4
A5	11.5	93	7.9	11
A6	13.7	1665.9	62.8	225.1
A7	12.55	2216.6	89.4	272.9
A8	13.14	1698.8	59.5	216.7
A9	11.47	2593.5	85.8	289.1
A10	11.5	1893.4	84.9	214.6

>TRINITY_DN107791_c1_g1_i3 from sample A2 / Example spliced isoform

CTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGAGGAAGCCATGCCTTCCATTCAAACAGCGCTTCCA
CCGGAGCTTGCCAACAATGTCAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGCAAGGTAACACAGAGCTGGTGGTTGATATGATTCGCCAACAGTTCAAGAAAAATATGCGCGAGACAGATCCAGAAAAGATACAAAAGATGAAAGATGAGTAAGTTCAGTTGTGCCTTTTGCTTAATTG
TGTTTTAGTTGCTCATGAGTTTATTAATTTTTTTTTATTGGAGTAATA

>TRINITY_DN107791_c1_g1_i5 from sample A2 / Example unspliced isoform:

CTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGAGGAAGCCATGCCTTCCATTCAAACAGCGCTTCCA
CCGGAGCTTGCCAACAATGTCAGAAACgtaagaaacactcacaagcatatcatatccctgttcagataaaacatttacacaactaattcgccccttcaatgcagATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGGTATGTATAGTACTTATTCTTTAAACCTTCTCCAGAACTAAATTTATTGCA
ATGCTCTGGTTACCTCGTCCACATTCTTTAACACCGTCTAAGCTGTATACCTTTTGGGTCTCAACAGTTTACTGACGGAAACCATAAAACCCTAGAAAGCTCCTGCCTCTCAATTTCAA

Intron 6 GTAAGTTCAG ... TGATGTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.69	215	0	56
A2	19.71	192.7	0	47
A3	24.16	268	0	85
A4	23.87	112	0	35
A5	15.89	85.3	0	16
A6	19.83	1871	0	461
A7	17.59	2025.7	0	430
A8	21.46	2540	0	693
A9	21.98	2619.3	4	734
A10	18.63	1524	0	346

>TRINITY_DN37548_c0_g1_i1 from sample A9 / Example spliced isoform

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCGGAGCTTGCCAACAATGTCAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGCAAGGTAACACAGAGCTGGTGGTTGATATGATTCGCCAACAGTTCAAGAAAAATATGCGCGAGACAGATCCAGAAAAGATACAAAAGATGAAAG
ATGATGCTGCGAGAGGCCTTGTAAATCACATGCTTTATGAATCAGAAAAAATGTCGGGACGCAAGTTTTCTACTCCCTAGAGGAATTGGTCATTTTACAATACCATGGCTATCACATTGTAGCTGACATGCATCCAAATTGTTGCAATGTTTTGTAAAACAGAATTGCAAGTTTAACTTTCTACCAAATAAAAACTATTA
GTTGGAACTCATTTTCTCCTCGTGTTATTTACTGTTGCAGATACCAATTTTATGAAAACTTTATGGAGTCTTACCATTAAAATTCAATTGTAATAGAGTATTGTATTCCACAATTGTATGATCACTAGAAACAATGATCGTAATGAAAGTGTTTTGTTTAGAAAGCTTATTCATCTTTTGTGTTCATGTAACTTGTGAAA
GCAGATCAATGCCATGTAGGTCTCCCG

>TRINITY_DN37548_c0_g1_i10 from sample A9 / Example unspliced isoform:

AAGATACAAAAGATGAAAGATGAgtaagttcagttgtgccttttgcttaattgtgttttagttgctcatgagtttattaattttttttattggagtaatagtatatcactgagttcatgctgtttgtgatgtctagTGCTGCGAGAGGCCTTGTAAATCACATGCTTTATGAATCAGAAAAAATGTCGGGACGCAAGTTT
TCTACTCCCTAGAGGAATTGGTCATTTTACAATACCATGGCTATCACATTGTAGCTGACATGCATCCAAATTGTTGCAATGTTTTGTAAAACAGAATTGCAAGTTTAACTTTCTACCAAATAAAAACTATTAGTTGGAACTCATTTTCTCCTCGTGTTATTTACTGTTGCAGATACCAATTTTATGAAAACTTTATGGAG
TCTTACCATTAAAATTCAATTGTAATAGAGTATTGTATTCCACAATTGTATGATCACTAGAAACAATGATCGTAATGAAAGTGTTTTGTTTAGAAAGCTTATTCATCTTTTGTGTTCATGTAACTTGTGAAAGCAGATCAATGCCATGTAGGTCTCCCG

Intron 7 GTGGGTAGCT ... GTTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.33	15	21	4
A2	61.15	61	96	2
A3	61.9	40	65	1
A4	63.16	28	48	0
A5	54.05	17	20	1
A6	63.51	235	409	0
A7	62.86	312	528	7
A8	62.58	226	378	5
A9	61.34	414	657	10
A10	61.35	264	419	1

>TRINITY_DN43930_c0_g1_i15 from sample A8 / Example spliced isoform

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACGTAAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGGATTTTTTGAAAATTTTGAACTTCATATGGAATCACGAAATTTACAGCAGTAGTGGCCATTTTTTAAAACAAGATTTTTTGAAAA
TTTTGAACTTCATATGGATTCACGAAATTTACAGCATTAGTGGCCATTTTCTTAAAAATGGACAAGTTCACATTTAGTGCCACATGAAGGGTTCTTTGATGTTCCAAGCATATTTGAGAAACACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCATAATTTTGTCCACAAAGACTTTGATTTT
GGAAGGCTTGAAAAAAAAGGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCAGCAGTAGTGGCCATTTTTATAAAAATGGACAAACTACGACTTTAGATTCCAGCAACACATTTACCTGTAACATCACATTTCCAGCCGTACATTATTTTTTGTTACAGCGTCACATTTGG
TGCCACATAAAGGGATCTTTGATGTTCAATGCATAGTTGAGAGGCACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCAGAATTTTGTCCACAAAGACTTTGGTTTTGGAAAGCTTGAAAAAAAAGGTTACAACTTTAGATTCCAACAACACACTATACTATACCATTTGTGGTTACAGAAGTT
ATAGTGGCCATTTTTATAAAAATTGATAAGTGTTATTTTTTAAATAATAGGTATTATTATTATTTGAGTTGGAAAATTCAATGTCTGTTTTATTGTTAAAATGATTGGTGATTTTGTTATTTTCGGGTTCCCTTTAGC

>TRINITY_DN43930_c0_g1_i12 from sample A8 / Example unspliced isoform:

CCTCGAGCCACCACGCTTCGCTGTGCTCTCTTACCTTAGATGTACGAAGTATAAGAAAATAAGCATGAAATGTGCACCTCCATTTATTTTTAAAGAAGCATCTCCGTGCTCTAAGCTAGATTTCATCTATAAAAACCAGAATACGGTTGTGTCAAAGAACAAGATCAAATCTCAGGGTACTTGGCAGGGGTAACGGAAGA
GGAAGCCATGCCTTCCATTCAAACAGCGCTTCCACCAGGGCTTGCCAACAATGTCAGAAACGTAAGAAACATTTATCGCGAGTGTCTTCGAAGAGCAAAGTTCGTCGGTCACAGGgtgggtagctgttagaatttacaatgtctcaaaaaagaaaaatccaagtcaccattggagaaatgtatggatggaaaaaagaaca
agaatccaaaattcaaaatgattcaaacactagtgaagaagcttataatactgaattggagcagcaattggatgatatttttggaagattcgctgatgaagatgtagatgaagaaagagatgaagttgaagaagaggttgataatgaaaagaaaatgaaagtgcaatataaaggaaattctgtatgggatggttagataa
gtttgattggctgaattatgtgaggcaggatgatatttagtatatatgaaatgcagggattgtgagaagtataggatgaatggtccatggggtttagggaatggttgtacgactttgcaacatgatgctcttgttactcatgaaaaatcattagtacataaagatgctaaagcaagatggattaatgcattggagagaaa
gatgaagccaattttagatcatatcaaacaagtggaagatgctaataagcaaagagttattgctacaatgagaatatcatattttgtatgccaagaagatttatccttatcaaagtacgaaaaactatgcaaatttctcatggatgcgaaaacaccaaatatgccaaagcctcaagagtattctttatacacaaatcgta
aatctgcaaatgatttcatatattccatatcaagacaccttgaagaggttcagataaagaatatgttggaaagtcctttcttctcattaatggttgatgaaagcacagatcgaagtttagagcagcaccttgttgtttatgcaacttatttagatgcaaagggtcttgggccacctatttcacaatttatgaagctgaaa
aatgtaatggatggaaaagggaaaacaatatatgatagtgtcaactagttaataaatgttagaggtcttcaatataaagatttgatagttgtttctacagatggggcttcagcaatgattggacatgaaaatggatttgtttcatttttgaaaaaagacactccaaatcttattacagcccattttgtagctcatcgcga
gtctctagcagcaacagatgcttctaaaaaaattcctgaactattatatgttgagaaaattgcaaacaaagtgtattcgtggattcaaaactcaccaaagagaagtaatgaacttaatgatttgcttaaagtcatgcagattgatgttctacaaatacacttagtgagatggttatctagggtggaagttattataaggc
ttgtgaagcttatgctagcgattttaacattgtgaaaaaatgaaagaaaaacttcatcatggtatgataaaacatgaatttattccattcagttttgtttgcatatgcttgctgatgtgttgggtgaattgaataagctgaacgagacttttcaagaagaaaatgcagacattacaactattgggttggctcttgaagta
actataagtacattgtctagatcgtttttgagaaaagaaacatttgcagaaggtacaacttatctttcaaaatttctttatgattcacaatatggatatattgaaattaaagatgaagggttagtcatagatagacatgagttacaatatatctctattccaactgcggatattgattatagtgattggaaacaaactat
gaaacttcatattgaaggaacacaagagagttgtcagcaactcacagaaggctatattgagtctctcatagactctttaaatggaagatttccatatcttcatctttttaatgcagcaaaattgttcatccattgttatgatccagaagaacgtcgtagccatgagaaaaattcagagcgttggctttctaaactttttc
agcatttgcagcataccttaccaaatggtgtcagaaatgtgcatttatttgatgaaaaagcttgcaaaagagaactgcactcttttgttgattcattaaatttggcttgtgagggtttctctatgaaagaagcatggaaggcctttcgtaatacaaaacaatggcacacaaattatcctcaaatgatgaaattatggcaa
gctgtactcactattcctacaagtacagtggattgtgaaaggggattttctaagcagaatataattaaggacattaggaaatcaaagctaggattggatacactagatgcactaatgagaatatttttgaatggattgaaattatcagctgttgattggaatgcagtgtatgagatatggagagatacaaagtcaagaag
agttcttgagctttgagagtagggaagtcttcctagtcctctcaagtgagttgtgtttagtttcaaaccaatgttttaagcaccgtgtgtatgtttttatattagatttaagcatttgtctctcatatcttatttttaatttaagaattgttcttgcagGATTTTTTGAAAATTTTGAACTTCATATGGAATCACGAAAT
TTACAGCAGTAGTGGCCATTTTTTAAAACAAGATTTTTTGAAAATTTTGAACTTCATATGGATTCACGAAATTTACAGCATTAGTGGCCATTTTCTTAAAAATGGACAAGTTCACATTTAGTGCCACATGAAGGGTTCTTTGATGTTCCAAGCATATTTGAGAAACACCTCTTTTTGTAAATCTGAGTCCAGCCATAACT
CAAAACCAAATCATCATAATTTTGTCCACAAAGACTTTGATTTTGGAAGGCTTGAAAAAAAAGGAATTTTGAAAATTTTGAACTTTCATATGGAATCATTTGTGGTTATAGAAGTTACAGCAGCAGCAGTAGTGGCCATTTTTATAAAAATGGACAAACTACGACTTTAGATTCCAGCAACACATTTACCTGTAACATCA
CATTTCCAGCCGTACATTATTTTTTGTTACAGCGTCACATTTGGTGCCACATAAAGGGATCTTTGATGTTCAATGCATAGTTGAGAGGCACCTCTTTTTGTAAATCTGAGTCCAGCCATAACTCAAAACCAAATCATCAGAATTTTGTCCACAAAGACTTTGGTTTTGGAAAGCTTGAAAAAAAAGGTTACAACTTTAGA
TTCCAACAACACACTATACTATACCATTTGTGGTTACAGAAGTTATAGTGGCCATTTTTATAAAAATTGATAAGTGTTATTTTTTAAATAATAGGTATTATTATTATTTGAGTTGGAAAATTCAATGTCTGTTTTATTGTTAAAATGATTGGTGATTTTGTTATTTTCGGGTTCCCTTTAGC