sp|Q9ZV87|NAPES_ARATH ----> N-acylphosphatidylethanolamine synthase

Intron 1 GTCAAACGGA ... GGAGCAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.35	9	8	11
A2	100	0	8	11
A3	41.18	10	3	11
A4	50	6	3	9
A5	-1	0	0	0
A6	51.65	22	11	36
A7	56.99	20	7	46
A8	53.7	25	16	42
A9	47.92	25	18	28
A10	50	23	24	22

>TRINITY_DN45011_c0_g1_i1 from sample A10 / Example spliced isoform

GAAACACAAGTACCTCCAGATGCTGTTTCAAGGGTGATTGGAGAGTGAAGACTGATCTCATTGGCTACCTTGTAGTCAAAACATTGTATGCCACGCACTCGGGAAGTCTAAGCGGTATTCGCATTTCCATTCCAAGGTCACACGCTCTCTGGAAAGAAAACACCAATGCTAATGTCCTACAAGATTCACAGCAGCAGGAG
GCAGGGAAGGATGGGGACTAATACAATGGAGTGGGCTGCAAAAGCCACCCATCTAGGGGGCATTCCTCGCCGATTGGTGATTAGAACAGTTGGTGCCTTCGCCAAGCTCATCACCTCCTATCTCAACTACACTCAAGTCCACAATGCAGACACTTTGCTCCAACTCATCAAATCCAGGCCTCCTGGCACCCCTCTCATCA
CAGTCAGCAATCACATGTCCACGT

>TRINITY_DN45011_c0_g1_i2 from sample A10 / Example unspliced isoform:

GAAACACAAGTACCTCCAGATGCTGTTTCAAGGGTGATTGGAGAGTGAAGACTGATCTCATTGGCTACCTTGTAGTCAAAACATTGTATGCCACGCACTCGGGAAGTCTAAGCGGTATTCTCATTTCCATTCTAAGGTCACACGCTCTCTGGAAAGAAAACACCAATGCTAATGTTCTACAAGATTCACAGgtcaaacgg
aacccaaatctccattttctactatttttaacagagggaaacccactcttgaaaccgggacacaaacagaaatcgctacaataagcaactctgatgtttggcagaatggcttgaggagcagcagCAGCAGGAGGCAGGGGAGGATGGGGACTAATACAATGGAGTGGGCTGCAAAAGCCACCCATCTAGGGGGCATTCCT
CGCCGATTGGTGATTAGAACAGTTGGTGCCTTCGCCAAGCTCATCACCTCCTATCTCAACTACACTCAAGTCCACAATGCAGACACTTTGCTCCAACTCATCAAATCCAGGCCTCCTGGCACCCCTCTCATCACAGTCAGCAATCACATGTCCACGTTGGATGATCCACTTATATGGGGGTTCAAGGGTTCTCCTATCAC
TGATTGGCAGCGAGCTCGTTGGACTCTCACAGCAGAGGACATTTGTTTCACAAATCCTTTCTTTACATACTTCTTTCGACTAGGAAAATGCATCCCTATTACAAGGGGTGCTGGTATTTACCAGACACATATGAATGAGGCTCTTGATCTCATTAATAAAGGAGAATGGTTGCACACATTTCCTGAAGGCAGTGTATCGC
AAGAGGGTCGTGTTATACGAAGATTAAAGTGGGGAACTGCAAGTCTTATTGTGCGTGCTTCTGTTACACCAATTGTTCTGCCTATTGTACACAGTGGGTTTGAAAAGGTAATGCCAGAGAAACATTGGTTCGGAAGGAGGCCACTTTTTCCTCTTTGTCTAAAAAACATTAACATTGTTGTTGGAGAGCCTCTGGAGTTT
GACCTCTCCACACTTCAGCAGAGAGCTGAGTCTGCGTCAAGAGTTCTTAGAGCAGAAGGGAATGGATACAATGAAGAAAATAGGGTCCATCTCGACAGCTATGGATGGCCAACGGGATTGTGTAAAGGATTAGATGAAATTGCGCAGAAATGGCTTTTTGTACATCTTTCAGATCACATTCGGAATGCCATGCAGAAACT
ATGCGTAAAAGCCAGGTCTCTTAATAATAGTGACCATAAATAATTTACATGCTCCTGTATCAGGGCAGGATTCAACATCAAAGAATTGCAATGTAATGTGGTAAAAAACATTCTGAATGTTATAACAGGAAAAGAGAAGTTAGGAATTGATGTTACAAGAGGTATAAAAACAGTCAATGTATTTTTCCATTTTGTTGATT
CTTATTAATAAAATGATTTTTTTTTTCTGGATTTAGAAA

Intron 2 GTTGGATGAT ... TTTCGACTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.45	25	27	20
A2	56.76	8	14	7
A3	63.27	9	18	13
A4	51.72	7	10	5
A5	78.95	2	4	11
A6	76.6	11	39	33
A7	59.42	28	33	49
A8	57.63	25	29	39
A9	61.54	25	36	44
A10	70.15	20	49	45

>TRINITY_DN40156_c0_g1_i2 from sample A8 / Example spliced isoform

GTTTCAAGGGTGATTGGAGAGTGAAGACTGATCTCATTGGCTACCTTGTAGTCAAAACATTGTATGCCACGCACTCGGGAAGTCTAAGCGGTATTCTCATTTCCATTCTAAGGTCACACGCTCTCTGGAAAGAAAACACCAATGCTAATGTTCTACAAGATTCACAGGTCAAACGGAACCCAAATCTCCATTTTCTACTA
TTTTTAACAGAGGGAAACCCACTCTTGAAACCGGGACACAAACAGAAATCGCTACAATAAGCAACTCTGATGTTTGGCAGAATGGCTTGAGGAGCAGCAGCAGCAGGAGGCAGGGGAGGATGGGGACTAATACAATGGAGTGGGCTGCAAAAGCCACCCATCTAGGGGGCATTCCTCGCCGATTGGTGATTAGAACAGTT
GGTGCCTTCGCCAAGCTCATCACCTCCTATCTCAACTACACTCAAGTCCACAATGCAGACACTTTGCTCCAACTCATCAAATCCAGGCCTCCTGGCACCCCTCTCATCACAGTCAGCAATCACATGTCCACGAAAATGCATCCCTATTACAAGGGGTGCTGGTATTTACCAGACACATATGAATGAGGCTCTTGATCTCA
TTAATAAAGGAGAATGGTTGCACACATTTCCTGAAGGCAGTGTATCGCAAGAGGGTCGTGTTATACGAAGATTAAAGTGGGGAACTGCAAGTCTTATTGTGCGTGCTTCTGTTACACCAATTGTTCTGCCTATTGTACACAGTGGGTTTGAAAAGGTAATGCCAGAGAAACATTGGTTCGGAAGGAGGCCACTTTTTCCT
CTTTGTCAAAAAAACATTAACATTGTTGTTGGAGAGCCTCTGGAGTTTGACCTCTCCACACTTCAGCAGAGAGCTGAGTCTGCGTCAAGAGTTCTTAGAGCAGAAGGGAATGGATACAATGAAGAAAATAGGGTCCATCTCGACAGCTATGGATGGCCAACGGGATTGTGTAAAGGATTAGATGAAATTGCGCAGAAATG
GCTTTTTGTACATCTTTCAGATCACATTCGGAATGCCATGCAGAAACTATGCGTAAAAGCCAGGTCTCTTAATAATAGTGACCATAAATAATTTACATGCTCCTGTATCAGGGCAGGATTCAACATCAAAGAATTGCAATGTAATGTGGTAAAAAACATTCTGAATGTTATAACAGGAAAAGAGAAGTTAGGAATTGATG
TTACAAGAGGTATAAAAACAGTCAATGTATTTTTCCATTTTGTTGATTCTTATTAATAAAATGATTTTTTTTTTCTGGATTTAAA

>TRINITY_DN40156_c0_g1_i4 from sample A8 / Example unspliced isoform:

GTTTCAAGGGTGATTGGAGAGTGAAGACTGATCTCATTGGCTACCTTGTAGTCAAAACATTGTATGCCACGCACTCGGGAAGTCTAAGCGGTATTCTCATTTCCATTCTAAGGTCACACGCTCTCTGGAAAGAAAACACCAATGCTAATGTTCTACAAGATTCACAGGTCAAACGGAACCCAAATCTCCATTTTCTACTA
TTTTTAACAGAGGGAAACCCACTCTTGAAACCGGGACACAAACAGAAATCGCTACAATAAGCAACTCTGATGTTTGGCAGAATGGCTTGAGGAGCAGCAGCAGCAGGAGGCAGGGGAGGATGGGGACTAATACAATGGAGTGGGCTGCAAAAGCCACCCATCTAGGGGGCATTCCTCGCCGATTGGTGATTAGAACAGTT
GGTGCCTTCGCCAAGCTCATCACCTCCTATCTCAACTACACTCAAGTCCACAATGCAGACACTTTGCTCCAACTCATCAAATCCAGGCCTCCTGGCACCCCTCTCATCACAGTCAGCAATCACATGTCCACgttggatgatccacttatatgggggttcaagggttctcctatcactgattggcagcgagctcgttggac
tctcacagcagaggacatttgtttcacaaatcctttctttacatacttctttcgactagGAAAATGCATCCCTATTACAAGGGGTGCTGGTATTTACCAGACACATATGAATGAGGCTCTTGATCTCATTAATAAAGGAGAATGGTTGCACACATTTCCTGAAGGCAGTGTATCGCAAGAGGGTCGTGTTATACGAAGAT
TAAAGTGGGGAACTGCAAGTCTTATTGTGCGTGCTTCTGTTACACCAATTGTTCTGCCTATTGTACACAGTGGGTTTGAAAAGGTAATGCCAGAGAAACATTGGTTCGGAAGGAGGCCACTTTTTCCTCTTTGTCAAAAAAACATTAACATTGTTGTTGGAGAGCCTCTGGAGTTTGACCTCTCCACACTTCAGCAGAGA
GCTGAGTCTGCGTCAAGAGTTCTTAGAGCAGAAGGGAATGGATACAATGAAGAAAATAGGGTCCATCTCGACAGCTATGGATGGCCAACGGGATTGTGTAAAGGATTAGATGAAATTGCGCAGAAATGGCTTTTTGTACATCTTTCAGATCACATTCGGAATGCCATGCAGAAACTATGCGTAAAAGCCAGGTCTCTTAA
TAATAGTGACCATAAATAATTTACATGCTCCTGTATCAGGGCAGGATTCAACATCAAAGAATTGCAATGTAATGTGGTAAAAAACATTCTGAATGTTATAACAGGAAAAGAGAAGTTAGGAATTGATGTTACAAGAGGTATAAAAACAGTCAATGTATTTTTCCATTTTGTTGATTCTTATTAATAAAATGATTTTTTTT
TTCTGGATTTAAA

Intron 3 GTCAAACGGA ... TGTTTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55	7	8.1	9
A2	73.87	3.6	8	12
A3	41.67	7	3	7
A4	42.86	8	3	9
A5	-1	0	0	0
A6	52.18	17	11.1	26
A7	66.56	9.6	7	31
A8	57.07	16.6	16	28
A9	50.52	21.6	18	26
A10	63.02	12.6	23.8	19

>TRINITY_DN40310_c0_g1_i3 from sample A6 / Example spliced isoform

TTTCAAGGGTGATTGGAGAGTGAAGACTGATCTCATTGGCTACCTTGTAGTCAAAACATTGTATGCCACGCACTCGGGAAGTCTAAGCGGTATTCGCATTTCCATTCCAAGGTCACACGCTCTCTGGAAAGAAAACACCAATGCTAATGTCCTACAAGATTCACAGAATGGCTTGAGGAGCAGCAGCAGCAGGAGGCAGG
GGAGGATGGGGACTAATACAATGGAGTGGGCTGCAAAAGCCACCCATCTAGGGGGCATTCCTCGCCGATTGGTGATTAGAACAGTTGGTGCCTTCGCCAAGCTCATCACCTCCTATCTCAACTACACTCAAGTCCACAATGCAGACACTTTGCTCCAACTCATCAAATCCAGGCCTCCTGGCACCCCTCTCATCACAGTC
AGCAATCACATGTCCACGTTGGATGATCCACTTATATGGGGGTTCAAGGGTTCTCCTATCACTGATTGGCAGCGAGCTCGTTGGACTCTCACAGCAGAGGACATTTGTTTCACAAATCCTTTCTTTACATACTTCTTTCGACTAGGAAAATGCATCCCTATTACAAGGGGTGCTGGTATTTACCAGACACATATGAATGA
GGCTCTTGATCTCATTAATAAAGGAGAATGGTGTATCGCAAGAGGGTGGTGTTATACGAAGATTAAAGTGGGGAACTGCAAGTCTTATTGTGCGTGCTTCTGTTACACCAATTGTTCTGCCTATTGTACACAGTGGGTTTGAAAAGGTAATGCCAGAGAAACATTGGTTCGGAAGGAGGCCACTTTTTCCTCTTTGTCAA
AAAAACATTAACATTGTTGTTGGAGAGCCTCTGGAGTTTGACCTCTCCACACTTCAGCAGAGAGCTGAGTCTGCGTCAAGAGTTCTTAGAGCAGAAGGGAATGGATACAATGAAGAAAATAGGGTCCATCTCGACAGCTATGGATGGCCAACGGGATTGTGTAAAGGATTAGATGAAATTGCGCAGAAATGGCTTTTTGT
ACATCTTTCAGATCACATTCGGAATGCCATGCAGAAACTATGCGTAAAAGCCAGGTCTCTTAATAATAGTGACCATAAATAATTTACATGCTCCTGTATCAGGGCAGGATTCAACATCAAAGAATTGCAATGTAATGTGGTAAAAAACATTCTGAATGTTATAACAGGAAAAGAGAAGTTAGGAATTGATGTTACAAGAG
GTATAAAAACAGTCAATGTATTTTTCCATTTTGTTGATTCTTATTAATAAAATGATTTTTTTTTTCTGGATTTTTTTTTTCTTGGACCCTTG

>TRINITY_DN40310_c0_g1_i1 from sample A6 / Example unspliced isoform:

TTTCAAGGGTGATTGGAGAGTGAAGACTGATCTCATTGGCTACCTTGTAGTCAAAACATTGTATGCCACGCACTCGGGAAGTCTAAGCGGTATTCTCATTTCCATTCTAAGGTCACACGCTCTCTGGAAAGAAAACACCAATGCTAATGTTCTACAAGATTCACAGgtcaaacggaacccaaatctccattttctactat
ttttaacagagggaaacccactcttgaaaccgggacacaaacagaaatcgctacaataagcaactctgatgtttggcagAATGGCTTGAGGAGCAGCAGCAGCAGGAGGCAGGGGAGGATGGGGACTAATACAATGGAGTGGGCTGCAAAAGCCACCCATCTAGGGGGCATTCCTCGCCGATTGGTGATTAGAACAGTTG
GTGCCTTCGCCAAGCTCATCACCTCCTATCTCAACTACACTCAAGTCCACAATGCAGACACTTTGCTCCAACTCATCAAATCCAGGCCTCCTGGCACCCCTCTCATCACAGTCAGCAATCACATGTCCACGTTGGATGATCCACTTATATGGGGGTTCAAGGGTTCTCCTATCACTGATTGGCAGCGAGCTCGTTGGACT
CTCACAGCAGAGGACATTTGTTTCACAAATCCTTTCTTTACATACTTCTTTCGACTAGGAAAATGCATCCCTATTACAAGGGGTGCTGGTATTTACCAGACACATATGAATGAGGCTCTTGATCTCATTAATAAAGGAGAATGGTTGCACACATTTCCTGAAGGCAGTGTATCGCAAGAGGGTGGTGTTATACGAAGATT
AAAGTGGGGAACTGCAAGTCTTATTGTGCGTGCTTCTGTTACACCAATTGTTCTGCCTATTGTACACAGTGGGTTTGAAAAGGTAATGCCAGAGAAACATTGGTTCGGAAGGAGGCCACTTTTTCCTCTTTGTCAAAAAAACATTAACATTGTTGTTGGAGAGCCTCTGGAGTTTGACCTCTCCACACTTCAGCAGAGAG
CTGAGTCTGCGTCAAGAGTTCTTAGAGCAGAAGGGAATGGATACAATGAAGAAAATAGGGTCCATCTCGACAGCTATGGATGGCCAACGGGATTGTGTAAAGGATTAGATGAAATTGCGCAGAAATGGCTTTTTGTACATCTTTCAGATCACATTCGGAATGCCATGCAGAAACTATGCGTAAAAGCCAGGTCTCTTAAT
AATAGTGACCATAAATAATTTACATGCTCCTGTATCAGGGCAGGATTCAACATCAAAGAATTGCAATGTAATGTGGTAAAAAACATTCTGAATGTTATAACAGGAAAAGAGAAGTTAGGAATTGATGTTACAAGAGGTATAAAAACAGTCAATGTATTTTTCCATTTTGTTGATTCTTATTAATAAAATGATTTTTTTTT
TCTGGATTTTTTTTTTCTTGGACCCTTG