sp|Q9SAV3|ODBB1_ARATH ----> 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial

Intron 1 GTCTGTTAAC ... TATTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.87	129	30	0
A2	31.58	78	36	1
A3	26.53	108	39	0
A4	22.77	78	23	0
A5	22.22	49	14	0
A6	26.52	751	271	0
A7	27.04	564	209	0
A8	24.79	707	233	0
A9	25.23	643	217	0
A10	26.88	457	168	0

>TRINITY_DN106271_c0_g1_i4 from sample A2 / Example spliced isoform

TGAGCACAGCTCAACAGATTTATCCCCAAAAAAACAATTATGCGAGGGAGCCTGAAGATTAAATATCTTGCTCAGCATTGCAGGTTTGGGTAGGCAGGTGTTGGTGGATTGAATTATTTCAGTGCAAGAGAGCCCGAAGATTAAAGATCTTGCTCAGCATTGCAGGTTTGGGTAGGCAGGTGTTGGTGGATTGAACTATT
TCAGTGCAAGAGAGGCGAACCCAGGTTGTTGAGGAGAAGCAATAGCAATGGCGACGATTGCGAGGAAACTGGGGTGGAGGAGGGCGATGAGCACGCTGTCGTCTTCATCTTCAATCCCATCTGTGAATTCGTCGATAATTCAATCAGAGGGGAAGCGAATTAATCTGTTTACGGCCGTAAATCAGGCCCTCCACATTGCT
CTCGAAACCGATCCTCGATCTTATGTGTTTGGGGAGGATGTGAGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTAACAGAGCGATTTGGTAAACATCGAGTTTTCAACACCCCCCTTTGTGAGCAGGGCATTGTTGGATTTGGAATTGGATTGGCTGCAATGGGAAATCGGGCAATAGTGGAAATTCAATTTGCAGA
TTACATTTACCCTGCATTTGACCAGATTGTGAATGAAGCTGCAAAGTATCGATATCGAAGTGGAAATCAATTTAACTGTGGAGGACTTACTATAAGAGCTCCTTATGGTGCTGTTGGCCATGGTGGCCACTATCACTCACAATCACCGGAGGCCTTCTTTTGTCATGCGCCGGGTATCAAGGTTGTTATACCACGGAGCC
CAATGCAAGCAAAAGGTTTACTACTTGCATCAATACGTGATCCAAATCCAGTGATCTTTTTTGAGCCAAAGTGGCTTTATCGTCTGGCAGTAGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGACGGATATCACATTAGTAGGATGGGGAGCTCAGCTTGCAATTATG
GAACAAGCTTGTCTGGATGCCAGTAAGGAGGGAATTTCCTGTGAGTTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTGAGAAAGACTGGGCGGCTTCTTGTTAGTCATGAGGCTCCTGTTACTGGTGGGTTTGGAGCAGAAATTGCTGCCTCTGTTGTGGAACGCTGTTTTCTAAG
GTTAGAAGCACCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGGTCTTAGATGCTATCAAAGCCACTGTAAATTACTAGCATTGCAGAATTTTTGCATCTGATTGCTCCACTGGAAAACAAGGCGCTTCTAGGAGTGTTCAGGTCTTTTGCATAG
AGTGCTGTACTTATGCCTGTAATTGACACAAATTAAATGGTTGAATGATTTACTTGACAAATATTGAAAATTCATAGTTCAATTATTGTACATAAAAACTGTATAATCGATTGAAACTATTTTGAAGGTCAATGGTGGGGGGGGGGAATTTGACCAGCTGAAATATTGATTTACTTGACAAATATTGAAAATTCAAAGTT
CAATTATTGTACATAAAAAAACTGTATAATCGATTGAAAATATTTTGAAGGTCAATGGGGGATTTGACTAGCAGAAATATTGTTGTTGGAAATAAAGATCTCTGAATGATGTTTC

>TRINITY_DN106271_c0_g1_i2 from sample A2 / Example unspliced isoform:

TGAGCACAGCTCAACAGATTTATCCCCAAAAAAACAATTATGCGAGGGAGCCTGAAGATTAAATATCTTGCTCAGCATTGCAGGTTTGGGTAGGCAGGTGTTGGTGGATTGAATTATTTCAGTGCAAGAGAGCCCGAAGATTAAAGATCTTGCTCAGCATTGCAGGTTTGGGTAGGCAGGTGTTGGTGGATTGAACTATT
TCAGTGCAAGAGAGGCGAACCCAGGTTGTTGAGGAGAAGCAATAGCAATGGCGACGATTGCGAGGAAACTGGGGTGGAGGAGGGCGATGAGCACGCTGTCGTCTTCATCTTCAATCCCATCTGTGAATTCGTCGATAATTCAATCAGAGGGGAAGCGAATTAATCTGTTTACGGCCGTAAATCAGGCCCTCCACATTGCT
CTCGAAACCGATCCTCGATCTTATGTGTTTGGGGAGGATGTGAGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTAACAGAGCGATTTGGTAAACATCGAGTTTTCAACACCCCCCTTTGTGAGCAGGGCATTGTTGGATTTGGAATTGGATTGGCTGCAATGGGAAATCGGGCAATAGTGGAAATTCAATTTGCAGA
TTACATTTACCCTGCATTTGACCAGATTGTGAATGAAGCTGCAAAGTATCGATATCGAAGTGGAAATCAATTTAACTGTGGAGGACTTACTATAAGAGCTCCTTATGGTGCTGTTGGCCATGGTGGCCACTATCACTCACAATCACCGGAGGCCTTCTTTTGTCATGCGCCGGGTATCAAGGTTGTTATACCACGGAGCC
CAATGCAAGCAAAAGGTTTACTACTTGCATCAATACGTGATCCAAATCCAGTGATCTTTTTTGAGCCAAAGTGGCTTTATCGTCTGGCAGTAGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGACGGATATCACATTAGTAGGATGGGGAGCTCAGCTTGCAATTATG
GAACAAGCTTGTCTGGATGCCAGTAAGGAGGGAATTTCCTGTGAGTTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTGAGAAAGACTGGGCGGCTTCTTGTTAGTCATGAGGCTCCTGTTACTGGTGGGTTTGGAGCAGAAATTGCTGCCTCTGTTGTGGAACGCTGTTTTCTAAG
GTTAGAAGCACCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGgtctgttaactgaatcaaatatgacatgtatcaaggcaaaaggatgctttatgaccatggaggtattttgacattgctgatttcgaacttgtctttatattttgcagGTCTT
AGATGCTATCAAAGC

Intron 2 GTGTCATCGT ... TTTGGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.18	135	1	30
A2	16.44	122	0	24
A3	10.47	154	1	18
A4	11.07	136.5	0	17
A5	13.58	70	0	11
A6	17.4	318	0	67
A7	15.03	350.5	0	62
A8	14.52	188.5	0	32
A9	21.09	310.5	0	83
A10	15.07	248	0	44

>TRINITY_DN97706_c12_g1_i5 from sample A4 / Example spliced isoform

GAAACATTGTACTCTTTTCTTTTTCAGTTTGAGAATTTTTAATTAACTTCCAAACATACAGATCTTATGTGTTTGGGGAGGATGTGAGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTAACAGAGCGATTTGGTAAACATCGAGTTTTCAACACCCCCCTTTGTGAGCAGGGCATTGTTGGATTTGGAATTGGATTG
GCTGCAATGGGAAATCGGGCAATAGTGGAAATTCAATTTGCAGATTACATTTACCCTGCATTTGACCAGATTGTGAATGAAGCTGCAAAGTATCGATATCGAAGTGGAAATCAATTTAACTGTGGAGGACTTACTATAAGAGCTCCTTATGGTGCTGTTGGCCATGGTGGCCACTATCACTCACAATCACCGGAGGCCTT
CTTTTGTCATGCGCCGGGTATCAAGGTTGTTATACCACGGAGCCCAATGCAAGCAAAAGGTTTACTACTTGCATCAATACGTGATCCAAATCCGGTGATCTTTTTTGAGCCAAAGTGGCTTTATCGTCTGGCAGTAGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGA
CGGATATCACATTAGTAGGATGGGGAGCTCAGCTTGCAATTATGGAACAAGCTTGTCTGGATGCCAGTAAGGAGGGAATTTCCTGTGAGTTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTGAGAAAGACTGGGCGGCTTCTTGTTAGTCATGAGGCTCCTGTTACTGGTGGGTTT
GGAGCAGAAATTGCTGCCTCTGTTGTGGAACGCTGTTTTCTAAGGTTAGAAGCACCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGGTCTTAGATGCTATCAAAGCCACTGTAAATTACTAGCATTGCAGAATTTTTGCATCTGATTGCTCCAC
TGGAAAACAAGGCGCTTCTAGGAGTGTTCAGGTCTTTTGCATAGAGTGCTGTACTTATGCCTGTAATTGACACAAATTAAATGGTTGAATGATTTACTTGACAAATATTGAAAATTCATAGTTCAATTATTGTACATAAAAACTGTATAATCGATTGAAACTATTTTGAAGGTCAATGGTGGGGGGGGGGAATTTGACCA
GCTGAAATATTGATTTACTTGACAAATATTGAAAATTCAAAGTTCAATTATTGTACATAAAAAAACTGTATAATCGATTGAAAATATTTTGAAGGTCAATGGGGGATTTGACTAGCAGAG

>TRINITY_DN97706_c12_g1_i3 from sample A4 / Example unspliced isoform:

TGGAATTGGATTGGCTGCAATGgtgtcatcgtgattcatgatgtatgtcacatgtaggcatgaacagagatgattaatggtcatcaacttctctgctgatttttgttaccgtcaatgtttgaaactagatgctatgcgaatgccgatttctgattttgccctgtttgtgttttggtgtagGGAAATCGGGCAATAGTGGA
AATTCAATTTGCAGATTACATTTACCCTGCATTTGACCAGATTGTGAATGAAGCTGCAAAGTATCGATATCGAAGTGGAAATCAATTTAACTGTGGAGGACTTACTATAAGAGCTCCTTATGGTGCTGTTGGCCATGGTGGCCACTATCACTCACAATCACCGGAGGCCTTCTTTTGTCATGCGCCGGGTATCAAGGTTG
TTATACCACGGAGCCCAATGCAAGCAAAAGGTTTACTACTTGCATCAATACGTGATCCAAATCCGGTGATCTTTTTTGAGCCAAAGTGGCTTTATCGTCTGGCAGTAGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGACGGATATCACATTAGTAGGATGGGGAGCT
CAGCTTGCAATTATGGAACAAGCTTGTCTGGATGCCAGTAAGGAGGGAATTTCCTGTGAGTTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTGAGAAAGACTGGGCGGCTTCTTGTTAGTCATGAGGCTCCTGTTACTGGTGGGTTTGGAGCAGAAATTGCTGCCTCTGTTGTGGA
ACGCTGTTTTCTAAGGTTAGAAGCACCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGGTCTTAGATGCTATCAAAGCCACTGTAAATTACTAGCATTGCAGAATTTTTGCATCTGATTGCTCCACTGGAAAACAAGGCGCTTCTAGGAGTGTTC
AGGTCTTTTGCATAGAGTGCTGTACTTATGCCTGTAATTGACACAAATTAAATGGTTGAATGATTTACTTGACAAATATTGAA

Intron 3 GTAAGTGCTG ... TTTGGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.18	135	39.7	28
A2	16.44	122	34	24
A3	10.98	154	48.3	19
A4	11.09	136.3	46.7	17
A5	13.58	70	15.7	11
A6	17.19	318	72	66
A7	14.62	350.3	105.3	60
A8	13.74	188.3	47.3	30
A9	20.29	310.3	74	79
A10	14.83	247	64.7	43

>TRINITY_DN113299_c0_g2_i2 from sample A1 / Example spliced isoform

GCACAGCTCAACAGATTTATCCCCAAAAAAACAATTATGCGAGGGAGCCTGAAGATTAAATATCTTGCTCAGCATTGCAGGTTTGGGTAGGCAGGTGTTGGTGGATTGAATTATTTCAGTGCAAGAGAGCCCGAAGATTAAAGATCTTGCTCAGCATTGCAGGTTTGGGTAGGCAGGTGTTGGTGGATTGAACTATTTCA
GTGCAAGAGAGGCGAACCCAGGTTGTTGAGGAGAAGCAATAGCAATGGCGACGATTGCGAGGAAACTGGGGTGGAGGAGGGCGATGAGCACGCTGTCGTCTTCATCTTCAATCCCATCTGTGAATTCGTCGATAATTCAATCAGAGGGGAAGCGAATTAATCTGTTTACGGCCGTAAATCAGGCCCTCCACATTGCTCTC
GAAACCGATCCTCGATCTTATGTGTTTGGGGAGGATGTGAGCTTTGGTGGGGTCTTCCGTTGCACAACTGGATTAACAGAGCGATTTGGTAAACATCGAGTTTTCAACACCCCCCTTTGTGAGCAGGGCATTGTTGGATTTGGAATTGGATTGGCTGCAATGGGAAATCGGGCAATAGTGGAAATTCAATTTGCAGATTA
CATTTACCCTGCATTTGACCAGATTGTGAATGAAGCTGCAAAGTATCGATATCGAAGTGGAAATCAATTTAACTGTGGAGGACTTACTATAAGAGCTCCTTATGGTGCTGTTGGCCATGGTGGCCACTATCACTCACAATCACCGGAGGCCTTCTTTTGTCATGCGCCGGGTATCAAGGTTGTTATACCACGGAGCCCAA
TGCAAGCAAAAGGTTTACTACTTGCATCAATACGTGATCCAAATCCGGTGATCTTTTTTGAGCCAAAGTGGCTTTATCGTCTGGCAGTAGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGACGGATATCACATTAGTAGGATGGGGAGCTCAGCTTGCAATTATGGAA
CAAGCTTGTCTGGATGCCAGTAAGGAGGGAATTTCCTGTGAGTTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTGAGAAAGACTGGGCGGCTTCTTGTTAGTCATGAGGCTCCTGTTACTGGTGGGTTTGGAGCAGAAATTGCTGCCTCTGTTGTGGAACGCTGTTTTCTAAGGTT
AGAAGCACCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGGTCTTAGATGCTATCAAAGCCACTGTAAATTACTAGCATTGCAGAATTTTTGCATCTGATTGCTCCACTGGAAAACAAGGCGCTTCTAGGAGTGTTCAGGTCTTTTGCATAGAGT
GCTGTACTTATGCCTGTAATTGACACAAATTAAATGGTTGAATGATTTACTTGACAAATATTGAAAATTCATAGTTCAATTATTGTACATAAAAACTGTATAATCGATTGAAACTATTTTGAAGGTCAATGGTGGGGGGCT

>TRINITY_DN113299_c0_g2_i1 from sample A1 / Example unspliced isoform:

TGGAATTGGATTGGCTGCAATGgtaagtgctgcatcttttgcatcatcttctcatgcctgctaattaatctagtatatgacctcttggcattttcaagatctaaaacattcatattcctttcagtttattggaggcacttattagagattgttagtatcaatatttgattacttcattaaactaattaatttcctcaggt
gtcatcgtgattcatgatgtatgtcacatgtaggcatgaacagagatgattaatggtcatcaacttctctgctgatttttgttactttcaatgtttgaaactagatgctatgcgaatgccgatttctgattttgccccgtttgtgttttggtgtagGGAAATCGGGCAATAGTGGAAATTCAATTTGCAGATTACATTTA
CCCTGCATTTGACCAGATTGTGAATGAAGCTGCAAAGTATCGATATCGAAGTGGAAATCAATTTAACTGTGGAGGACTTACTATAAGAGCTCCTTATGGTGCTGTTGGCCATGGTGGCCACTATCACTCACAATCACCGGAGGCCTTCTTTTGTCATGCGCCGGGTATCAAGGTTGTTATACCACGGAGCCCAATGCAAG
CAAAAGGTTTACTACTTGCATCAATACGTGATCCAAATCCGGTGATCTTTTTTGAGCCAAAGTGGCTTTATCGTCTGGCAGTAGAAGAAGTCCCTGACCATGATTATACATTGCCCTTGTCGCAGGCAGAAGTGATTCGGGAAGGGACGGATATCACATTAGTAGGATGGGGAGCTCAGCTTGCAATTATGGAACAAGCT
TGTCTGGATGCCAGTAAGGAGGGAATTTCCTGTGAGTTAATAGACTTGCGAACCTTGATTCCATGGGACAAGGAGACAGTTGAGGCATCTGTGAGAAAGACTGGGCGGCTTCTTGTTAGTCATGAGGCTCCTGTTACTGGTGGGTTTGGAGCAGAAATTGCTGCCTCTGTTGTGGAACGCTGTTTTCTAAGGTTAGAAGC
ACCAGTTGCAAGGGTTTGTGGTCTAGATACTCCATTCCCATTGGTTTTTGAGCCCTTCTACATGCCCACAAAGAATAAGGTCTTAGATGCTATCAAAGCCACTGTAAATTACTAGCATTGCAGAATTTTTGCATCTGATTGCTCCACTGGAAAACAAGGCGCTTCTAGGAGTGTTCAGGTCTTTTGCATAGAGTGCTGTA
CTTATGCCTGTAATTGACACAAATTAAATGGTTGAATGATTTACTTGACAAATATTGAAAATTCATAGTTCAATTATTGTACATAAAAACTGTATAATCGATTGAAACTATTTTGAAGGTCAATGGTGGGGGGCT