sp|Q42396|CDPKC_ARATH ----> Calcium-dependent protein kinase 12

Intron 1 GTATGTTGCA ... AATTTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.25	128.5	80	93
A2	43.03	131	51	98
A3	50.2	96.5	37	96
A4	44.28	97.5	54	77
A5	51.24	63	52	66
A6	39.12	204	13	124
A7	37.7	303	25	170
A8	37.58	256	5	144
A9	41.42	343	14	223
A10	36.11	278	17	148

>TRINITY_DN111877_c1_g1_i3 from sample A4 / Example spliced isoform

CCCGGAACAAGATACGTAGAATCTGCCTGTAAAAAGATGCCATATAATGAGATAGATGTAAAAAGATGCCATATAATGAGATAGATATACAGAGGACCCCAGGAATCTTGAGGATCAGAGCCCACTGCTGCCCGGAACAAGATACATTGTCAAAGAATGTCTACATGTTTTCAGAGCCCACTGCTGCCCGGAACAAGATA
CATTGTCAAAGAATGTCTACATGTTTTCAGAGCTCTGCGATGGAGGAGATCTCAGAGACAGACAACCATGGCCACGGTATTCTTTCTCAGAGACAGACAACCAGAATAATCCAATCTTTTGTCCAGTCCATTAAATTCTGCCAATCGAGGAAAATCATTCACAGAGTATTTTTATAGCAAACAGTGTAAACTCTTCATTC
AAGGGCTTTAATCAAACTTGTAAATTTTGATAAGTCGATTTGGAAAGGTAATCTAAATTAGTCATCGACGAGAACGTGGGGACATTGGGATATTCTGCTCCAGAGATGCTGAGCGGCAATGGCTACTCGTACAAGGCTGATATATGGAGCGCGGGAGTTGTGTTGTATGTTCTTCTCTGTGGTAAGAAGCCTTTCGATGT
GGATAATGAAGACGCCAACAGCATGTA

>TRINITY_DN111877_c1_g1_i11 from sample A4 / Example unspliced isoform:

ATTGCAACGGTCCGAACTCCCATTGCCAGGGATTATGAGATTGGGGAGGGAACTACTCTCAAGGACGGTTGGGAGTAGTGCACAAGTGCATTCACAGGACAACCAGAGTAGAATCTGCCTGTAAAAAGATGCCATATAATGAGATAGATGTAAAAAGATGCCATATAATGAGATAGATATACAGAGGACCCCAGGAATCT
TGAGGATCAGAGCCCACTGCTGCCCGGAACAAGATACATTGTCAAAGAATGTCTACATGTTTTCAGAGCCCACTGCTGCCCGGAACAAGATACATTGTCAAAGAATGTCTACATGTTTTCAGAGCTCTGCGATGGAGGAGATCTCAGAGACAGACAACCATGGCCACGGTATTCTTTCTCAGAGACAGACAACCAGAATA
ATCCAATCTTTTGTCCAGTCCATTAAATTCTGCCAATCGAGGAAAATCATTCACAGAGATGTAAAGCCATCGAGTATTTTTATAGCAAACAGTGTAAACTCTTCATTCAAGGGCTTTAATCAAACTTGTAAATTTTGATAAGTCGATTTGGAAAGgtatgttgcagaaatattcttcaagattgatttgtcaattgcagt
atgccgtagtatattgacggattctttattaatttgtcagGTAATCTAAATTAGTCATCGACGAGAACGTGGGGACATTGGGATATTCTGCTCCAGAGATGCTGAGCGGAAATGGGTACTCGTACGAGGCTGATATATGGAGCGCGGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGATGTGGAGAAT
GAAGACGCCAACAGCATGTATGATAAAATACAGAATACAAAACCAGAT

Intron 2 GTGAGTTATT ... GGTCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.43	263	48	13
A2	16.35	261	51	4
A3	16.94	309	63	9
A4	19.13	224	53	11
A5	14.39	119	20	9
A6	16.02	2338	446	63
A7	17.16	3833	794	59
A8	16.38	3910	766	114
A9	15.7	4413	822	82
A10	14.65	3467	595	48

>TRINITY_DN46703_c3_g1_i3 from sample A8 / Example spliced isoform

TGGCAAGGCAATATAAATGTTGTGAGCGATAACGTGGGGACGTTGGGATTTTCTGCTCCAGAGATGCTGCGCGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTCGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGTTGCTGAGAATGAGGACGCCAATAGCATGTATGATAAAATACAGAA
TACAAAACCAGACAGTAGCGGTGTATGGAGGTACATATCCGATTCTGTAAAAGAACTCGTTCTTGGAATGTTGCAAACGGACCCGATGCTGCGATTTTCTTGCCATCAAATCTTGAGTCATCCTTGGATTATGGCCCATGCAGAGATGAACTTGCAGATTCCCAGTTCCACCATCAACAGTACAGAGAGAATGGTTCCAC
CCCGGAGGCCGTTGCCGGACACAATTAATTATGAAAAGACCGTTGGCAGGCGAACACAGAGCAGAAAGAAGAGCCATCCGATTCCGATTCCGATTGTGTGTCCAAGAAAGGAATAGCAAATTGCTCATATCAGCTCATTTGAAATATATTTATGAAACAGTACGTCACCAAATGTTTAATTCGATAGTCTTTCCTCTGCT
GTGCCGTAAA

>TRINITY_DN46703_c3_g1_i21 from sample A8 / Example unspliced isoform:

TGGCAAGGCAATATAAATGTTGTGAGCGATAACGTGGGGACGTTGGGATTTTCTGCTCCAGAGATGCTGCGCGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTCGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGTTGCTGAGAATGAGGACGCCAATAGCATGTATGATAAAATACAGAA
TACAAAACCAGACAGTAGCGGTGTATGGAGGTACATATCCGATTCTGTAAAAGAACTCGTTCTTGGAATGTTGCAAACGGACCCGATGCTGCGATTTTCTTGCCATCAAATCTTGAgtgagttattattttctttggattttggattttgtgaatatgtaagctttgtttcttccgaatggcaaatcttgaatgagtcat
tcttttctttggattttagattttgtgggtatgtaaagagggatttgatttagtggagttttaatttttttactatgtcaagaatggtttgacttgataaatttggatttaatataaatttattgattttaatttttttataaaaaattttaactccttttaattttcttaacatttacaatttctcccataaactacat
tgtaaagcattttgttactcaggtccaataacttagtggtatcagtatgttaatatgttcaaattttttttactctagtgtgggtatcagtttgttaatatgttggtatgcaaaatttatttctaaataaaattaggggtttagggttttataattggagtggcatatttcaaattgttttggttagggttttataattg
cactggattttgcaaaatttgtttctatataaaattagggttttataattggagtggcatatttcaaattgttttggttagggttttataattgcactcgcctatttaaaattgatttagttggtgcaatatcggtataggtttaaaaaaaaccatttatcaacaatcattgcaattccacagaaatatatatatatata
taggtactttagtagaattaacatatatctatactgtcttttagtttatgaccggagataaatatttttctctactttttatttttgtaacgtttttaacaaatcttccctccagattacagaaagttcgaactgcattggtttttataaaatatccacgatgaccttcggtgttattagctattaaatagatttttggg
aaaaaaatatgtatgtacatgtctttgctttttaagagtgaatctgtatataatctcaatgtaaagataaaactaaaggagcagtgatagagaactaaattggcatttgagcgtggtcatgcagGTCATCCTTGGATTATGGCCCATGCAGAGATGAACTTGCAGATTCCCAGTTCCACCATCAACAGTACAGAGAGAAT
GGTTCCACCCCGGAGGCCGTTGCCGGACACAATTAATTATGAAAAGACCGTTGGCAGGCGAACACAGAGCAGAAAGAAGAGCCATCCGATTCCGATTCCGATTGTGTGTCCAAGAAAGGAATAGCAAATTGCTCATATCAGCTCATTTGAAATATATTTATGAAACAGTACGTCACCAAATGTTTAATTCGATAGTCTTT
CCTCTGCTGTGCCGTAAA

Intron 3 GTGAGTTATT ... GCCCCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.17	44	48	9
A2	49.04	53	51	2
A3	49.61	64	63	7
A4	56.38	41	53	14
A5	50	20	20	4
A6	50.11	444	446	51
A7	54.61	660	794	57
A8	49.2	791	766	97
A9	52.16	754	822	78
A10	53.8	511	595	38

>TRINITY_DN46703_c3_g1_i2 from sample A8 / Example spliced isoform

TGGCAAGGCAATATAAATGTTGTGAGCGATAACGTGGGGACGTTGGGATTTTCTGCTCCAGAGATGCTGCGCGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTCGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGTTGCTGAGAATGAGGACGCCAATAGCATGTATGATAAAATACAGAT
TACAAAACCAGACAGTAGTGGTGTATGGAGGTACATATCGTATTCTGTAAAAGAACTCGTTCTTGGAATGTTGCAAACGGACCCGATGCTGCGATTTTCTTGCCATCAAATCTTGAAGATGAACATGCAGATTCCCAGCTCCACCATCAACAGTACAGAGAGAATGGTTCCACCCCGGAGGCGAACACAGAGCAGAAAGA
GGAGCCATCCGATTCCGATTTATAGCAAATTGCTCATATCAGCTCATGTTTGAAATATATTTATGAAACAGTACGTCACCAAATGTTTAATTCGATACTCTCCTCTGCTATCCCGTAAAAATTGCACGCTTCAGGATACTGAGCCTCACGAATTCTTTATATTGATATCTTTGTATGAAAGAAGTATATTACAATTGCAA
AGTTTGATATTAATCTGGTTGAAATTAATCAAATCAGACTGAATCATAAGTTTTGTCGTATTTTTAGTTACAAGTCAAGTGACATCACAGTATATTTTGGCTTAACAGTGAGTGATCTATG

>TRINITY_DN46703_c3_g1_i23 from sample A8 / Example unspliced isoform:

TGGCAAGGCAATATAAATGTTGTGAGCGATAACGTGGGGACGTTGGGATTTTCTGCTCCAGAGATGCTGCGCGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTCGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGTTGCTGAGAATGAGGACGCCAATAGCATGTATGATAAAATACAGAA
TACAAAACCAGACAGTAGCGGTGTATGGAGGTACATATCCGATTCTGTAAAAGAACTCGTTCTTGGAATGTTGCAAACGGACCCGATGCTGCGATTTTCTTGCCATCAAATCTTGAgtgagttattattttctttggattttggattttgtgaatatgtaagctttgtttcttccgaatggcaaatcttgaatgagtcat
tcttttctttggattttagattttgtgggtatgtaaagagggatttgatttagtggagttttaatttttttactatgtcaagaatggtttgacttgataaatttggatttaatataaatttattgattttaatttttttataaaaaattttaactccttttaattttcttaacatttacaatttctcccataaactacat
tgtaaagcattttgttactcaggtccaataacttagtggtatcagtatgttaatatgttcaaattttttttactctagtgtgggtatcagtttgttaatatgttggtatgcaaaatttatttctaaataaaattaggggtttagggttttataattggagtggcatatttcaaattgttttggttagggttttataattg
cactggattttgcaaaatttgtttctatataaaattagggttttataattggagtggcatatttcaaattgttttggttagggttttataattgcactcgcctatttaaaattgatttagttggtgcaatatcggtataggtttaaaaaaaaccatttatcaacaatcattgcaattccacagaaatatatatatatata
taggtactttagtagaattaacatatatctatactgtcttttagtttatgaccggagataaatatttttctctactttttatttttgtaacgtttttaacatatcttcccttccagattactgaaagttcgaagtgcattggtttttataaaatatccacgatgaccttctgtatttttagctattaaatagatttttgg
gaaaaaatatgtagatgcatgtccttgctttttaagagtgaatgtgtatataatctcaatgtaaagataaaactaaaggaacagtgatagagaactaaattggcatttgagggattatggcccctgcagAGATGAACATGCAGATTCCCAGCTCCACCATCAACAGTACAGAGAGAATGGTTCCACCCCGGAGGCGAACA
CAGAGCAGAAAGAGGAGCCATCCGATTCCGATTTATAGCAAATTGCTCATATCAGCTCATGTTTGAAATATATTTATGAAACAGTACGTCACCAAATGTTTAATTCGATACTCTCCTCTGCTATCCCGTAAAAATTGCACGCTTCAGGATACTGAGCCTCACGAATTCTTTATATTGATATCTTTGTATGAAAGAAGTAT
ATTACAATTGCAAAGTTTGATATTAATCTGGTTGAAATTAATCAAATCAGACTGAATCATAAGTTTTGTCGTATTTTTAGTTACAAGTCAAGTGACATCACAGTATATTTTGGCTTAACAGTGAGTGATCTATG

Intron 4 GTATGTGGCA ... ATTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.05	304	57	68
A2	16.04	246	25	69
A3	15.08	276	47	51
A4	13.78	244	32	46
A5	15.33	116	16	26
A6	13.29	1305	154	246
A7	13.69	2204	279	420
A8	13.86	2002	292	352
A9	13.76	2654	370	477
A10	12.03	2260	261	357

>TRINITY_DN122501_c0_g1_i5 from sample A1 / Example spliced isoform

GCCATAATAGTCCAATCTCTTGTCCAATCCCTTAAATTATGCCAATTGCGGAAAATCATTCACAGAGACGTAAAACCATCGAGTATTTTTAGTGCAAACAATGTAAATTCTTCTTCATGGGCTTTTAAACTTGGAAATTTTGATTTGGCGGTTTGGAAAGGTAATATAAATATTGTCATCGATAACGTGGGGACGTTGGG
ATATTCTGCTCCAGAGATGCTGCGTGGAAATGGGTACTCGTACGAGGCTGATATATGGAGCGCGGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGATGCTGAGAATGAGAACGCGAATAGCATGTATCATAAAATACAGAATACAAAACTAGATCTCAGCAGCGGTGTATGGAGGTATATATCCGATT
CTGTAAAGAAACTCGTTCTTGGAATGTTGCAAACGGACCCGATGCGGCGACTCTCTTGTGATCAAATCTTGAGTCATCCTTGGATTCTGGCCTGTACAGAGAAGAACTCACCTCCCAGCTCCAGCATCAACACAGAGATAATGGTTCCATCTCAGAGGTCGTTGCTGGCCATCATTAATGAATAGACCGTTTACAGATGA
ACACCGATCAGAAAGAAAAGCCATACGATCTTGTGTGTAAGAAAATAATAGCAAATTGCTGATATCACTTCATCTTGAAAGCTATTTATGAAACCGTCCGTCACCGTGCCGTCGTCAAATGTTTTCGTTCTGTATTCTCTCCTCTGCTCTGCCGTAGAAAATGCAGACTTCAGGATATTGATTGAGCCATTCAATCACTT
TGAACGAACAGATTTGTTTGAGGCTCATGAATTCTATATATTGGTATCTTTGAATGAAAACATTATATTACATTTTCCAAATTTTGATATTAATCGGGTTGAAAATCAAACATTCAGATTCAGTTTTGTG

>TRINITY_DN122501_c0_g1_i6 from sample A1 / Example unspliced isoform:

GCCATAATAGTCCAATCTCTTGTCCAATCCCTTAAATTATGCCAATTGCGGAAAATCATTCACAGAGACGTAAAACCATCGAGTATTTTTAGTGCAAACAATGTAAATTCTTCTTCATGGGCTTTTAAACTTGGAAATTTTGATTTGGCGGTTTGGAAAGgtatgtggcagcaatatttgtctagattaatttgtcaatt
tgaagtatgtggtagtatattgacggattctttatttttacagGTAATATAAATATTGTCATCGATAACGTGGGGACGTTGGGATATTCTGCTCCAGAGATGCTGCGTGGAAATGGGTACTCGTACGAGGCTGATATATGGAGCGCGGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGATGCTGAGAA
TGAGAACGCGAATAGCATGTATCATAAAATACAGAATACAAAACTAGATCTCAGCAGCGGTGTATGGAGGTATATATCCGATTCTGTAAAGAAACTCGTTCTTGGAATGTTGCAAACGGACCCGATGCGGCGACTCTCTTGTGATCAAATCTTGAGTCATCCTTGGATTCTGGCCTGTACAGAGAAGAACTCACCTCCCA
GCTCCAGCATCAACACAGAGATAATGGTTCCATCTCAGAGGTCGTTGCTGGCCATCATTAATGAATAGACCGTTTACAGATGAACACCGATCAGAAAGAAAAGCCATACGATCTTGTGTGTAAGAAAATAATAGCAAATTGCTGATATCACTTCATCTTGAAAGCTATTTATGAAACCGTCCGTCACCGTGCCGTCGTCA
AATGTTTTCGTTCTGTATTCTCTCCTCTGCTCTGCCGTAGAAAATGCAGACTTCAGGATATTGATTGAGCCATTCAATCACTTTGAACGAACAGATTTGTTTGAGGCTCATGAATTCTATATATTGGTATCTTTGAATGAAAACATTATATTACATTTTCCAAATTTTGATATTAATCGGGTTGAAAATCAAACATTCAG
ATTCAGTTTTGTG

Intron 5 GCAAGTTGAA ... TATTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.37	34	25	2
A2	32.43	25	12	4
A3	27.27	40	15	1
A4	33.33	18	9	0
A5	53.33	14	16	2
A6	35.58	134	74	3
A7	36.18	127	72	6
A8	36.26	116	66	7
A9	32.28	172	82	8
A10	28.96	211	86	23

>TRINITY_DN45160_c1_g1_i12 from sample A9 / Example spliced isoform

TAACATTTCTATGAAGAGCAGGGCAAGCATTCAAATATCCGTCCTGCAATTTTAGAATTCGAACATATCATCCATCGTCATCTTTCTTCTCAAGAAACTGTGGTGTTAAATTAGTATTGTCAATAATTCCTACGCTGAAACCATGCTGCATGCGATACAATTTTTGTGGAGTTGTTGCAGAGTACGTTATGATGAGGACA
CCGAAGGATCTGGCGCCGGAAATCCCGAACTTCTTGCGGAGAAGAATTACGAACTTGGGGAGGTGATTGGTGAAGGACGGTTGGGTGTGGTATACAAGTGCATTCGCAGGTCAACCGGAGAACAACTCGCCTGCAAAAAGATGCCCTCTACGATCAAGAATGCATATGATGAGATGCTTTTACTGGAAAACCATTTATCC
GGCAAACACCCAGGAATCTTGAAGGTCAGGGCCTGCTACTGGGTGGAGCGGCTAAGCCTCTATAACTGGTCAAGGTATGTCTACATGATTTCCGAGCTCTGCGAAGGGGGAGATCTCCAGCGCTTTCTTGAAAGAAATGGCATCCCTTCAGAGAGGGAAGCCGCCGGAATAATGAAATGTATCGTGGAAGCTCTTCGGTA
CTGCCATTCGCAGAGAATTATTCATAGAGACGTAAAACCATCAAGCATTTTTATATTCAACTCCGGCTCACCGTGGGTTGTTAAACTCGGAAATTTCGATCGATCAATTCCGTTTCCACGAGCAGGGAATGTTGTAATCAAAGAAAACGTGGGAGCACGGGGATATTCTGCACCAGAGATGCTGCTTGGAAATGGGTACT
CGTACGAGGTTGATATATGGAGCGCGGGAGTTGTGTTGTATGTTCTTCTCTGTGGGAAGAAGCCTTTTGATGCTGAGAATGAGGACGTGAATAGCATGTATCATAAAATACAGATTACAAAACCAGACAGTAGTGGTGTATGGAGGTACATATCGTATTCTGTAAAAGAACTCGTTCTTGGAATGTTGCAAACGGACCCG
AAGCTGCGATTTTCTTGCCATCAAATCTTGAGTGAGTTATTATTTTCTTTGGATTTTGTTGGTATGCAAAATTTATTTCTAAATAAAATTAGGGGTTTAGGGTTTTATAATTGGAGTGGCATATTTCAAATTGTTTTGGTTAGGGTTTTATAATTGCACTGGATTTTGCAAAATTTGTTTCTATATAAAATTAGGGTTTT
ATAATTGGAGTGGCATATTTGAAATTGTTTTGGTTAGGGTTTTATAATTGCACTCGCCTATTTAAAATTGATTTAGTTGGTGCAATATGGGTATAGATTTAAAAAAAATCCATTTATCAACGATCATTACAATTCCACAG

>TRINITY_DN45160_c1_g1_i6 from sample A9 / Example unspliced isoform:

TAACATTTCTATGAAGAGCAGGGCAAGCATTCAAATATCCGTCCTGCAATTTTAGAATTCGAACATATCATCCATCGTCATCTTTCTTCTCAAGAAACTGTGGTGTTAAATTAGTATTGTCAATAATTCCTACGCTGAAACCATGCTGCATGCGATACAATTTTTGTGGAGTTGTTGCAGAGTACGTTATGATGAGGACA
CCGAAGGATCTGGCGCCGGAAATCCCGAACTTCTTGCGGAGAAGAATTACGAACTTGGGGAGGTGATTGGTGAAGGACGGTTGGGTGTGGTATACAAGTGCATTCGCAGGTCAACCGGAGAACAACTCGCCTGCAAAAAGATGCCCTCTACGATCAAGAATGCATATGATGAGATGCTTTTACTGGAAAACCATTTATCC
GGCAAACACCCAGGAATCTTGAAGGTCAGGGCCTGCTACTGGGTGGAGCGGCTAAGCCTCTATAACTGGTCAAGGTATGTCTACATGATTTCCGAGCTCTGCGAAGGGGGAGATCTCCAGCGCTTTCTTGAAAGAAATGGCATCCCTTCAGAGAGGGAAGCCGCCGGAATAATGAAATGTATCGTGGAAGCTCTTCGGTA
CTGCCATTCGCAGAGAATTATTCATAGAGACGTAAAACCATCAAGCATTTTTATATTCAACTCCGGCTCACCGTGGGTTGTTAAACTCGGAAATTTCGATCGATCAATTCCGTTTCCACGAGgcaagttgaaaaattattctcttttgccatctcttttgcaagtggcctggtaattcaagaaacatcgagtgagaaatc
ttccaagaaattcaaaacaccgattagcacatcagctgattcagacttattgaatattttttatttttcagCAGGGAATGTTGTAATCAAAGAAAACGTGGGAGCACGGGGATATTCTGCACCAGAGATGCTGCTTGGAAATGGGTACTCGTACGAGGTTGATATATGGAGCGCGGGAGTTGT