sp|Q500V5|AGDP1_ARATH ----> Protein AGENET DOMAIN (AGD)-CONTAINING P1


Intron 1 GTTTATTTCT ... TGCTGCACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A117.48852610
A217.5380268
A320.55872718
A418.57571016
A522.2228610
A60100
A716.67520
A8-1000
A925320
A1025302



>TRINITY_DN122282_c1_g1_i2 from sample A2 / Example spliced isoform
AGGGTATCACAGGTTGAAGTAGTACTTTTGACTTTTGCTGAACTAGCTGTATAGAGTATGTCATAACAAACATCTTGGTCTAATCCTGTCTTTGCCATTGGAGAAGCTGCATTGCTGTTATATACCAACTTTTTGGTTCGATATACTAAATTGTAGAATATGAAAATTGCATTAACCTTTTGTTGGATTTAAACTGGTAA
CTCTTTTTGCTTGTAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAG
CATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCA
GGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGC
AGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACA
ATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATC
TCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTG
GCATAATTGACAGCCATAGAGATATTGAATCTCATGGCCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTTGCA
GTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTA
TGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTC
CACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGA
GAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGA
TGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATA
GTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGGG
CAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAA
AGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATC
AAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAAT
GAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCAC
ATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACT
CAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCA
TTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTC
TGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAG
GTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAA
GCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGT
GCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTG
CAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGC
CATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAG
GCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCAT
CTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGT
GGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGA
AACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAG
CAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCT
ACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAA
TGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAA
GTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCT
GATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGG
CACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGA
TTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAG
GAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAG
GCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATC
TAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTT
TCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACG
TAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATA
TGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGT
GTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTT
AAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATA
ATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAA
TTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAATTATTTTGCTTTCTGTTATTTTTGAACCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGC


>TRINITY_DN122282_c1_g1_i8 from sample A2 / Example unspliced isoform:
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGTGAGTGTTTTCATTCTC
TCCCCAAACCCTGTTATTTTTTATTCTCAAGGTTTTGTTCTGGAGACGTGTAAAGGAGATGCCTTGGATTTCGGCAGCTTAAAGTGTTAAAATTGGGTTTCATTAACTTTGGGTACTGTCATTTAAACTTAACGCAACGGGATGTAGCCGCGCGCTGTCAATCTTACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCG
GTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACA
AGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCC
ACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGA
ATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATT
CTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGCCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTT
GCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAA
CTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATG
TTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACG
GGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAA
TGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGG
ATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGAT
GGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGT
TAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATG
ATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAA
AATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGG
CACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCG
ACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATC
TCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAA
GTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGgtttatttctgtttaccaagtgaagcttgtt
ttccttatgtcaataggatggcgttcttcatagagaacaacttttacaaatgaagcttatgttcgttacaatgtcacttggaatgcttgaatgtgctgtgttcattgaagccagagtcctttcctttttgtaaaagcttcagattatttcagtgcatgggtcatattcctttcatgtttattggttgtggtgggtccttt
ctttagctttgcagacttacaactaggttgtttattgaattagtcttatttttactggatttgctgcacagAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTT
GCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCT
TTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATG
AGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATT
GTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACT
CACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGG
CCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACA
GATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAA
TGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTC
AGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTAT
TCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGA
TCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCT
CAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGG
GAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGA
TGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCA
TTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAG
CTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCT
CACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAG
TTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAG
GATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATC
GGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCAC
TTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATC
TGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGC
CTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATG
AAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGG
ACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAATTATTTTGCTTTCTGTTATTTTTGAA
CCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGC



Intron 2 GTAATATATG ... CTGTTTACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A174.03102532
A255.26172715
A369.23102817
A474.194203
A557.1491212
A652.94568343
A764.76379442
A858.745910167
A964.44245829
A1057.41233527



>TRINITY_DN122282_c1_g1_i11 from sample A2 / Example spliced isoform
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGTGAGTGTTTTCATTCTC
TCCCCAAACCCTGTTATTTTTTATTCTCAAGGTTTTGTTCTGGAGACGTGTAAAGGAGATGCCTTGGATTTCGGCAGCTTAAAGTGTTAAAATTGGGTTTCATTAACTTTGGGTACTGTCATTTAAACTTAACGCAACGGGATGTAGCCGCGCGCTGTCAATCTTACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCG
GTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACA
AGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCC
ACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGA
ATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATT
CTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGCCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTT
GCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAA
CTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATG
TTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACG
GGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAA
TGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGG
ATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGAT
GGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGT
TAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATG
ATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAA
AATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGG
CACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCG
ACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATC
TCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAA
GTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGA
AAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGT
CAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATC
AGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTG
CTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGG
AGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGC
AAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTT
CATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACT
GGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGA
GGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTG
CAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCT
GCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCA
TAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCT
TAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTC
TCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGA
AGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCAT
TGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAAT
GAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCC
CAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAG
ATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCT
GTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCC
ACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATC
ATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTT
TCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGA
AAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTG
CTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAA
TTATTTTGCTTTCTGTTATTTTTGAACCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGC


>TRINITY_DN122282_c1_g1_i1 from sample A2 / Example unspliced isoform:
TTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTAC
GCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATgtaatatatgatttcataattcttaataccttctctccatctgtgtcaaatgatttgaaaatgctggagcaggtgaatttctgttttataacctttcagttattttt
cacattgcctgttgaagttttctttaggggtgttttaggtttgaggcttatatggcaatattgttctctgtttacagTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATG
ACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGT
GCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTG
TCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAATTATTTTGCTTTCTGTTATTTTTGAACCCATTTTAATGGTCATGGTTGTAATG
AGAGTTGTTAGGTGC



Intron 3 GTTAGATTTT ... TCCTATACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A121.849925.330
A220922422
A323.21862527
A412.3846310
A539.13211710
A630.77935
A725.47602021
A819.136107
A923.88511022
A1020.9932710



>TRINITY_DN123461_c0_g1_i11 from sample A1 / Example spliced isoform
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCACTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGTGAGTGTTTTCATTCTC
TCCCCAAACCCTGTTATTTTTTATTCTCAAGGTTTTGTTCTGGAGACGTGTAAAGGAGATGCCTTGGATTTCGGCAGCTTAAAGTGTTAAAATTGGGTTTCATTAACTTTGGGTACTGTCATTTAAACTTAACGCAACGGGATGTAGCCGCGCGCTGTCAATCTTACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCG
GTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACA
AGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCC
ACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGA
ATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATT
CTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACTCAGCTGTT
GCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAA
CTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATG
TTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACG
GGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAA
TGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGG
ATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGAGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGC
AGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGA
TTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGA
AAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCA
ACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTAT
TACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTT
TACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGA
GGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGAT
TGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATA
ATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGT
CCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGT
ACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCT
CCGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAAC
ATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCC
CACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTC
CTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCT
GCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGC
TGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTAC
AAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCTTTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGG
GCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGA
AAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAG
CACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCC
AAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTA
TATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTT
TAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAG
GAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAA
GCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGG
CAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCT
ACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTT
GTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGC
AGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCA
CTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAAT
TGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGAT
ATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAAC


>TRINITY_DN123461_c0_g1_i4 from sample A1 / Example unspliced isoform:
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCACTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGAATCTGCAAATGGAGTA
TGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCAC
ACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCA
TGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGA
TAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGACATTGAATTACATGGTC
TAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACACAACTTAACAAAGACCAT
TTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCATAGAGATATTGAATCTCA
TGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACTCAGCTGTTGCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGAC
AGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGG
CAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGT
ATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGA
CAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAACATACTGTAGACACTTTG
ATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAA
GGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGAGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAG
CAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGT
CTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAA
CAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTT
CCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAG
ATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGG
AGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAA
ATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTAT
GGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACT
CCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTG
GGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTT
ATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCCGACATAACTGCTCATACTCCTTGCTCGAGCACAAC
AGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAA
TTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATG
CCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCA
CACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAA
TGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGG
CACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGA
TGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCTTTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAG
GAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGA
GATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGT
TGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCC
GTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATT
CGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGgttagatt
ttttattatatcaattcttttattttggacatgcttgccataacaatttgttttaggtaaaatatcatgacatgaactagtatttctgtgatttacaaatgaataatgaatgttttcctatacagGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAG
TTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCAT
TAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAA
CTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTT
GGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCC
TCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAA
ATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATT
TCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGGTGAATTTCTGTTTTATAACCTTTCAGTTATTTTTCACATTGCCTGTTGAAGTTTTCTTTAGGGGTGTTTTAGGTTTGAGGCTTATATGGCAATATTGTTCTCTGTTTACAGTGTAGTTGTTCAGCAAAATTTGAGGACAG
ATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACA
TACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGT
TGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAAC



Intron 4 GTGAATTTCT ... CTGTTTACAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A129.31411730.9
A236.36352014.1
A336.59261515.8
A439.5326172.8
A523.0810311.1
A633.07854240.1
A735784239.4
A837.32895362.5
A927.45742827.2
A1035.59382125.3



>TRINITY_DN123461_c0_g1_i9 from sample A1 / Example spliced isoform
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCACTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGAATCTGCAAATGGAGTA
TGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCAC
ACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCA
TGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGA
TAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGACATTGAATTACATGGTC
TAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACACAACTTAACAAAGACCAT
TTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCATAGAGATATTGAATCTCA
TGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACTCAGCTGTTGCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGAC
AGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGG
CAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGT
ATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGA
CAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAACATACTGTAGACACTTTG
ATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAA
GGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGAGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAG
CAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGT
CTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAA
CAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTT
CCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAG
ATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGG
AGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAA
ATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTAT
GGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACT
CCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTG
GGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTT
ATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCCGACATAACTGCTCATACTCCTTGCTCGAGCACAAC
AGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAA
TTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATG
CCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCA
CACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAA
TGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGG
CACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGA
TGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCTTTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAG
GAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGA
GATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGT
TGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCC
GTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATT
CGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGTTAGATT
TTTTATTATATCAATTCTTTTATTTTGGACATGCTTGCCATAACAATTTGTTTTAGGTAAAATATCATGACATGAACTAGTATTTCTGTGATTTACAAATGAATAATGAATGTTTTCCTATACAGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAG
TTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCAT
TAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAA
CTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTT
GGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCC
TCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAA
ATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATT
TCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAA
GTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTT
GAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTT
CATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAAC


>TRINITY_DN123461_c0_g1_i4 from sample A1 / Example unspliced isoform:
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCACTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGAATCTGCAAATGGAGTA
TGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCAC
ACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCA
TGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGA
TAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGACATTGAATTACATGGTC
TAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACACAACTTAACAAAGACCAT
TTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCATAGAGATATTGAATCTCA
TGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACTCAGCTGTTGCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGAC
AGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGG
CAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGT
ATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGA
CAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAACATACTGTAGACACTTTG
ATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAA
GGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGAGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAG
CAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGT
CTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAA
CAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTT
CCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAG
ATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGG
AGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAA
ATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTAT
GGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACT
CCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTG
GGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTT
ATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCCGACATAACTGCTCATACTCCTTGCTCGAGCACAAC
AGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAA
TTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATG
CCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCA
CACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAA
TGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGG
CACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGA
TGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCTTTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAG
GAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGA
GATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGT
TGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCC
GTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATT
CGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGTTAGATT
TTTTATTATATCAATTCTTTTATTTTGGACATGCTTGCCATAACAATTTGTTTTAGGTAAAATATCATGACATGAACTAGTATTTCTGTGATTTACAAATGAATAATGAATGTTTTCCTATACAGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAG
TTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCAT
TAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAA
CTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTT
GGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCC
TCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAA
ATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATT
TCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGgtgaatttctgttttataacctttcagttatttttcacattgcctgttgaagttttctttaggggtgttttaggtttgaggcttatatggcaatattgttctctgtttacagTGTAGTTGTTCAGCAAAATTTGAGGACAG
ATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACA
TACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGT
TGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAAC



Intron 5 GTAATATATG ... GCTGGAGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A180.39104141
A268.22173835
A374.68103326
A486.4442526
A55591210
A662.915610585
A772.393711678
A862.425910789
A976.47248274
A1062.6233938



>TRINITY_DN122282_c1_g1_i11 from sample A2 / Example spliced isoform
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGTGAGTGTTTTCATTCTC
TCCCCAAACCCTGTTATTTTTTATTCTCAAGGTTTTGTTCTGGAGACGTGTAAAGGAGATGCCTTGGATTTCGGCAGCTTAAAGTGTTAAAATTGGGTTTCATTAACTTTGGGTACTGTCATTTAAACTTAACGCAACGGGATGTAGCCGCGCGCTGTCAATCTTACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCG
GTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACA
AGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCC
ACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGA
ATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATT
CTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGCCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTT
GCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAA
CTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATG
TTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACG
GGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAA
TGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGG
ATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGAT
GGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGT
TAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATG
ATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAA
AATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGG
CACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCG
ACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATC
TCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAA
GTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGA
AAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGT
CAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATC
AGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTG
CTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGG
AGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGC
AAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTT
CATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACT
GGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGA
GGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTG
CAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCT
GCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCA
TAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCT
TAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTC
TCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGA
AGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCAT
TGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAAT
GAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCC
CAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAG
ATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCT
GTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCC
ACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATC
ATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTT
TCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGA
AAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTG
CTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAA
TTATTTTGCTTTCTGTTATTTTTGAACCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGC


>TRINITY_DN122282_c1_g1_i8 from sample A2 / Example unspliced isoform:
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGTGAGTGTTTTCATTCTC
TCCCCAAACCCTGTTATTTTTTATTCTCAAGGTTTTGTTCTGGAGACGTGTAAAGGAGATGCCTTGGATTTCGGCAGCTTAAAGTGTTAAAATTGGGTTTCATTAACTTTGGGTACTGTCATTTAAACTTAACGCAACGGGATGTAGCCGCGCGCTGTCAATCTTACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCG
GTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACA
AGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCC
ACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGA
ATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATT
CTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGCCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTT
GCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAA
CTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATG
TTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACG
GGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAA
TGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGG
ATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGAT
GGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGT
TAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATG
ATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAA
AATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGG
CACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCG
ACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATC
TCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAA
GTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGGTTTATTTCTGTTTACCAAGTGAAGCTTGTT
TTCCTTATGTCAATAGGATGGCGTTCTTCATAGAGAACAACTTTTACAAATGAAGCTTATGTTCGTTACAATGTCACTTGGAATGCTTGAATGTGCTGTGTTCATTGAAGCCAGAGTCCTTTCCTTTTTGTAAAAGCTTCAGATTATTTCAGTGCATGGGTCATATTCCTTTCATGTTTATTGGTTGTGGTGGGTCCTTT
CTTTAGCTTTGCAGACTTACAACTAGGTTGTTTATTGAATTAGTCTTATTTTTACTGGATTTGCTGCACAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTT
GCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCT
TTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATG
AGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATT
GTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACT
CACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGG
CCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACA
GATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAA
TGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTC
AGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTAT
TCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGA
TCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCT
CAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGG
GAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGA
TGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCA
TTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAG
CTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCT
CACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAG
TTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAG
GATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATC
GGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCAC
TTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATC
TGGAAGCACTGTCCTCCATCAAAATCGTACATgtaatatatgatttcataattcttaataccttctctccatctgtgtcaaatgatttgaaaatgctggagcagTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGC
CTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATG
AAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGG
ACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAATTATTTTGCTTTCTGTTATTTTTGAA
CCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGC



Intron 6 GTGAGTGTTT ... GAGGAGAGAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A128.28713620
A228.81633318
A323.18582510
A419.62842615
A531.9132228
A629.41614
A7-1000
A8-1000
A9-1000
A1033.33321



>TRINITY_DN123461_c0_g1_i9 from sample A1 / Example spliced isoform
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCACTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGGAATCTGCAAATGGAGTA
TGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCAC
ACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCA
TGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGA
TAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGACATTGAATTACATGGTC
TAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACACAACTTAACAAAGACCAT
TTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCATAGAGATATTGAATCTCA
TGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACTCAGCTGTTGCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGAC
AGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGG
CAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGT
ATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGA
CAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAACATACTGTAGACACTTTG
ATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAA
GGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGAGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAG
CAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGT
CTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAA
CAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTT
CCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAG
ATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGG
AGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAA
ATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTAT
GGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACT
CCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTG
GGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTT
ATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCCGACATAACTGCTCATACTCCTTGCTCGAGCACAAC
AGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAA
TTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATG
CCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCA
CACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAA
TGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGG
CACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGA
TGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCTTTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAG
GAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGA
GATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGT
TGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCC
GTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATT
CGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGTTAGATT
TTTTATTATATCAATTCTTTTATTTTGGACATGCTTGCCATAACAATTTGTTTTAGGTAAAATATCATGACATGAACTAGTATTTCTGTGATTTACAAATGAATAATGAATGTTTTCCTATACAGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAG
TTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCAT
TAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAA
CTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTT
GGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCC
TCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAA
ATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATT
TCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAA
GTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTT
GAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTT
CATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAAC


>TRINITY_DN123461_c0_g1_i11 from sample A1 / Example unspliced isoform:
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCACTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGgtgagtgttttcattctc
tccccaaaccctgttattttttattctcaaggttttgttctggagacgtgtaaaggagatgccttggatttcggcagcttaaagtgttaaaattgggtttcattaactttgggtactgtcatttaaacttaacgcaacgggatgtagccgcgcgctgtcaatcttacaagatttagtaggccgggagagcaggtgaagcg
gttcgagtagaggagagagGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACA
AGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCC
ACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGA
ATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATT
CTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACTCAGCTGTT
GCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAA
CTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATG
TTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACG
GGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAA
TGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGG
ATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGAGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGC
AGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGA
TTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGA
AAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCA
ACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTAT
TACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTT
TACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGA
GGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGAT
TGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATA
ATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGT
CCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGT
ACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCT
CCGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAAC
ATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCC
CACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTC
CTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCT
GCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGC
TGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTAC
AAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCTTTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGG
GCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGA
AAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAG
CACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCC
AAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTA
TATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTT
TAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAG
GAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAA
GCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGG
CAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCT
ACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTT
GTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGC
AGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCA
CTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAAT
TGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGAT
ATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAAC



Intron 7 GTGAGTGTTT ... CGGGATGTAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A164.2920369
A261.82213412
A360.9816259
A460.4717265
A570.979226
A650110
A7-1000
A8-1000
A9-1000
A10100020



>TRINITY_DN122282_c1_g1_i17 from sample A2 / Example spliced isoform
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGCCGCGCGCTGTCAATCTT
ACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCGGTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGA
GGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCT
TCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGG
CTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGCTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTG
CTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGA
TTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAA
TGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGCCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAA
TGCCAGACAATGATGCTGGACAGTACACAGCTGTTGCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCC
TCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTTCAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAA
AGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTG
CGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTG
CAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACA
AATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGA
CCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGA
TTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGA
TATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAA
AACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAAT
CAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATT
TTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGG
CCGAGGCAACGGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAA
AAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGAT
TCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGA
AGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTACGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACA
TTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCTGCATCAGATGG
AGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTT
CAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGG
AGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCACCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAAC
TCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAA
AGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGT
AGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGT
GGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGG
AAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACC
CAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTG
TTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTA
ATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATT
CGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATAC
ATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAG
AGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTT
CAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAA
AAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTG
CCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACATAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAA
GTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGC
AGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTCATAATTCTTAATACCTTCTC
TCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGGGAATTAATATAGCTGCTC
TTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAAAGTAGTGTCTTAAATAAA
TTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATTCTACTGTCCAATAGTTCG
TACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAATTATTTTGCTTTCTGTTATTTTTGAACCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGC


>TRINITY_DN122282_c1_g1_i4 from sample A2 / Example unspliced isoform:
GCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGATAGATTGATGGTCGATT
TGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGgtgagtgttttcattctc
tccccaaaccctgttattttttattctcaaggttttgttctggagacgtgtaaaggagatgccttggatttcggcagcttaaagtgttaaaattgggtttcattaactttgggtactgtcatttaaacttaacgcaacgggatgtagCCGCGCGCTGTCAATCTTACAAGATTTAGTAGGCCGGGAGAGCAGGTGAAGCG
GTTCGAGTAGAGGAGAGAGGAATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTT
CAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGG
TCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATAAAGCATTTTTAATATAGCTAACGTGATGCTGATTTCTATTGATTTATCTGGGTTGTTGTGGGTATAGTGGATTTTTTGCGGAAATTTTCACTTATTTTTCTTTATTTCTCGAACCCCGAAACTAACACCCACCCC
TAGTTGGCTGCGATCTGTGACTTTGGTGTGTGCAGCGGCTGTGATTTTAAAACGCCATTATTTCCTGTTTCTTATATTCGATAAGATTGTTTTCAGTCTAAAACGAGCCGTTTGTGTTTCTGATTTTTTTATTTTTAATGAAATACCCGTTGTGTAGACGAAGAGACCTGGAAACTCTGATACTTTTTTCTTCTTTTGCC
AGTGAAACGGCTTTTGTTTAGACAAACGGGCCGTGTAACTCCGTTTTGTTTTCAAAGGGAAAAAACTGGGCACAACAACTGCGTAAATGCGTCCCCCTTTACTCTGCTTTAATGAAGAACCCGAAACTCCGAATTTGTTTCCCAGTGATACTCCCTTTTCCCATGATACTCCTTTTTGTTAACTACCGGAACACATCGGG
TACATTGACTGCGTAAATGCGGCCCTCTGTCCCTGCCTTAAGGCTGCCTCTCTTTTTTCTGCTTTAAGGCTGCCCCCGATCAGGTGCGACACCCCCGCTAGTTTTTAAACAAGGGTCATTGTGGTGATAAGCGTCTGCACTTACCACTAGACAACAGCCTCAGACCTCTGCATTTCCCAACTAACACATCGACCCAATAA
AAAAGGTCCTGTAGTAATCGTGTTCTTATTCTGCCTCTGTAAAGCTCTTTTTGGAATTCTGCTAGTTGTTTGTAGAGTTCTTGTTGGAATTCTGCTAGTTTTGTAGGGCTCTTGTTGGAATTCTGCTAGTTGTCTGTAGGGCCCTTGTCAGAATTCTGCCAGTTGTCTCTAGTGTTATGTTGGAATTCCAAAAGAAATTT
TCAAAGGCTGTGTCTCCGGTATTTCAATCCCTGATCGGCAATATCTTGCTGAAACATATGCTTGGAACTTTGCAGAATCTGGCTGATTGTTAACTGTCCGTAATTAAATTTCGTTTTGTTGGATGATGGTTCGGTATTTCGTTTCCCGCGAAAGAGATGCGAAATGAGCAACATCCGATTTTTGTAAGCAGCTTGTGCAG
ACCTTGCAAACAACAGTGTGTACCTAACAAACATGGAGCTATAAATGAGTGTTCAGCCTTGCCAGCTCAAGAAAGAAAAAGTCTTGCTGTAAGTGGTAAGGCATTCATTTCTTTTTGCTACGGGCATTTATAGCTTGCTAGTTGAGGTTTGGGGCATAGTAGCAAGATTCGGTTGTATTGGCCTTTGCTATACAGAAATT
CAGCATTGGTACAAAATTTGGCCCCTATTCTGCACAGTTCAGATATGGTAAGGTCAACATGTTTTTTACTGTGTTTGATAAGATGCTTCATTTCTTAGATAGCTGTCTACTGAAAACCCCCCAAAATTGCCCAAGAATCTCAAACCAAAACCCTAAATAAACTTTTAAAGATAAACTATGTACCAAATTTCCAAAGAAAT
TATTTTTTCTTATTTATTACATGTTTTTGAGGCTGAATTTTTTCTAATATTTGGAACAAAAAGCATATTTTTCC



Intron 8 GTCGTCTAAA ... AATAATGCAG

SampleRetention score (%)Exon junction reads5'ss reads3'ss reads
A116.471041526
A218.64721122
A318.99101.714.733
A411.116488
A523.2338815
A620201
A716.8147217
A823.7517.7011
A917.3650615
A1015.694379



>TRINITY_DN115609_c0_g1_i5 from sample A3 / Example spliced isoform
GATTAAAGATATTTCATGCGTATGGGGTTTTGTCTGAGTGGGGAAGAACGAGAAAAGGAGGAGACCGGCGGGGGCTTGTACGTACTGCGGGTTTTTATTTTACTTGCGTTTCTGTCTGCATAAACCCTGCACAAAAGGGTCGAGGTTCGGCAAATTAGTTCACGGCGACTGAATTTTCGAGCCCTCTGCACCTTAGCCGA
TAGATTGATGGTCGATTTGCAGGAAATGGAGCTCGGGATGTGGTCCGGCGAGTGAACGAGGGTTTCGTAGCTATATTTTTAGAAGCGGGACTCGAAAAGAGAGACTTTGGGGTTTTCGCTGCAGGAGATGATCTCGCAAGGAGGAGAATAGCTGTGTTCCGTGCACCGAAGACTCAGCTCCAGCTTCGAATGGAGGGAGG
AATCTGCAAATGGAGTATGATGACATTAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGA
GGAAAACTGGAGTTCACACTTTTCCAGGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTG
TTGAGGGACCAGATGCATGTGAGTCATTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTG
TCTGAACCTCCTGAAGATAACAGCTTGGAGCTGAAGGTTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGA
CATTGAATTACATGGTCTAGAATCAGAACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACAC
AACTTAACAAAGACCATTTGGCTGACATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCAT
AGAGATATTGAATCTCATGGTCTACAAACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTTGCAGTTTCTTCTCAAGAAAC
ACAGGCTGATTCTGGACAGACAAAATTGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATA
TTGTATTGGCTAACTGGCAAGGAGGGCCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTACAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAG
GGAATGCCAGAATTTGTATCAGCTAAAATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGA
AGTCAGTTACGAAAAGACAGAACAGCCTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAAC
ATACTGTAGACACTTTGATGGAGGTTGATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCC
AGAGATTTTTCTTGCAAGGATATTAAGTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGA
AACTAGTGTGCCTTTAGCAGAAAAGACCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTC
CTTATGCACCGTCTAGTCTTCTTGATCATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTA
GGCAGCTTAGATGAAAACAGTTCCAAGAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGT
GGTCAAAGCTACTTCTTCCCCAGAAGGAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATA
GCAAGCACACTGATCAGATGAATCTTGAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAA
AGAAGTGTTATTTTGGGAGCACAAGAAGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACTGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAG
TTGTTTGCTGCTTCCAAATGTGGATCGGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTG
CTGAAGAGGGTATCTATGGCTCAGTAGATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCT
GATACAATTTCAGTACTCCAGGAAGACAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTATGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAG
CCATTCATCTAAAAGTGGGGCTAGACAGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTC
TTGTGTACGGGTCTCTTATTCAAGGCTCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCGGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACT
CCTTGCTCGAGCACAACAGGAACAGTATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAA
AAATACCCCCCGGGCAATTATGAGTCCACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACAC
CTTGGATGCCTCACATGCCTTTCCCTGGGCCATGGGTGGCCCCTCCTCAAGGACCGCCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTG
GGTGCTACTACAGGTCACACTAGTACCAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGC
TTCCCCTGGGTCGTCAATGATTGTCTCAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTG
CAGCTACTGCAGTTAGGCACAGCCAGGGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACT
GAGGCTCTGAGTTCTGATGGTAAACAAGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACA
AGCTGTATCACAAGCAGGAGCTGTTGTGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATA
AATTCAAAGATAAGAGAGATGTGTTCCGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTT
GCTGTAGCTGTCTCTGTTGCAGAGACCATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGA
TGGGAAAGCTTTTGTCCGTTATGATGAACTACTTTCAGATGAAGGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATAATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATC
ATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCATTTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTA
GCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTTGCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGA
GCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTAAAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGG
TTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACTGCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAA
AGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGGGCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGC
AACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACGTAGAAAAGGGCATACTTCCTCAGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGT
CACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAATACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTA
CCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTCATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAGGTGAATTTCTGTTTTATAACCTTTCAGTTATTTTTCACATTGCCTGTTGAAGTTTTC
TTTAGGGGTGTTTTAGGTTTGAGGCTTATATGGCAATATTGTTCTCTGTTTACAGTGTAGTTGTTCAGCAAAATTTGAGGACAGATATGCCACTTTTTGCTAACTGGAAAGCTATTAGTGCCTTTTCAAGCCTCTCAGGATTGTGTTAAGCCTGAATGTAAAAAGTGGTTTTTACATGACTAAGTGGATATCAAAAGTGG
GAATTAATATAGCTGCTCTTGTTCCTTCCGAGGTTTAAACAGAGATTTAGAACGTTAAGATTGTTGATTATCATGTTGAAAACATACCCAATTGGCAGGAAGGCTGCAACAAAAAGGTGATCCGAAAAGATCCCCATGTATAATTTCAATGAAATGTAATATTGTAAAGTACATTTGTGCAGATCCACGCTGGGTTGAAA
AGTAGTGTCTTAAATAAATTTAGGGTAGGACCATTAGTACCTTGTAGGTAGTAATAATTTCTTTACTCAAACACATTGACGTGTTGAAGGATATTTGCATATTGCTCGATAATAGAAAGTTCTTGCTGGTGTAACGAGGTTATTCTTCAGGACGACTTTCCTAGATGAAAAGAATTTGTCGGGATTTTCGAATGTTCATT
CTGCTGTCCAATAGTTCGTACTTCATTCTACACTGTTAATGTTGAACCTCAACAAATTTCTGTTGTTTGATTATATGTATTCTGTTCAAAAGAGTGGCTACTCAATTCTAGCTTTAATATGCTAATTATTTTGCTTTCTGTTATTTTTGAACCCATTTTAATGGTCATGGTTGTAATGAGAGTTGTTAGGTGCTATTTTA
TGAACCACGTTGTAACTGCAACTGATGTTTGAACAGCCTGCTCCTTGACACATTGAGGGGGGATCGAACTCATGATCTGAAAAGTATATTTTTTCATTTGTCATTAAGGGAAATCTCATGGTTGTCCCCATTTTACTGTGAATTTGATTTGAAATTTCATCCTTAGTGCACGAT


>TRINITY_DN115609_c0_g1_i1 from sample A3 / Example unspliced isoform:
TAGCTGTATAGAGTATGTCATAACAAACATCTTGGTCTAATCCTGTCTTTGCCATTGGAGAAGCTGCATTGCTGTTATATACCAACTTTTTGGTTCGATATACTAAATTGTAGAATATGAAAATTGCATTAATCTTTTGTTGGATTTAAACTGGTAACTCTTTTTGCTTGTAGGAATCTGCAAATGGAGTATGATGACAT
TAATTTCATAGACAGAGATTCTCAAGTGGTGGATAAGGACAAGCATCACTCAGACGCAGAGGAGGGTCAAGTTTGCCGCCCCAGTTTTGATCTAGTTGAACATCTAGGAGTGCAGTTGAGGTTTGATAGTCTCGACGAAGCTAGCGTTCTACTTCAGCATCAAAGCCAGAGAGAGGAAAACTGGAGTTCACACTTTTCCA
GGGAGGAGAATGGTGTTCTTGGCCTCGAGGCTGGTTCACAGAATATAGAGTTCAGTTCAACTGCCACGCAAACTTGCACCATTTCAAGGCATGTTAATGTTTGGTCTGAGGCAACATCTTCAGAGTCTGTACAAATGCTTTTGAATTCAGTTGGTCAGGATGAAGAGAACCTTGTTGAGGGACCAGATGCATGTGAGTCA
TTGCAAACCTCAATGAATGACAAGGACTTTAACATGTCAAATAGTGAATCTTCAGCATGTAATATAGAGGCCGTCTTAGACGTAGAGTGCAGGACTGCATTTGAAAAAACCCAGCAAGGCTTTGAAAATCCAGTAATGGATGTAAGGGATGACAAGCAGCAACTTCCAATTTTGTCTGAACCTCCTGAAGATAACAGCTT
GGAGCTGAAGGTTGTTTCAGACCTTGACATTAGGCCCACAGATAAGAGACATTTCCCAACCAAAGAGGATGCTTCTGAAAAAAGTAGCGTTGAGAGAGAGAGTTCTGCACCATTAGGTGCTTCAATAGAAGATTTGAATGCGAGATCACCAGCCACAATCGAAAGTGATAGAGACATTGAATTACATGGTCTAGAATCAG
AACTGAATGATGTTCCATTGTCTGGCTTCATCGCTTCTTCTGAGACTGGCAGTCTGAAAGAGATAAAGATAGAGAGTTCTGCTATACAAGGTGCACCATTACTAGATGTGAATGCTAGATTGCCAGGCATAATTGACAGCCATAGAGATATTGAATCTCATGATCTAGAAACACAACTTAACAAAGACCATTTGGCTGAC
ATTACCCCTTCAGATGAGACTGGCAGTCAGACAGAGATGGGGGATACAAGACAAGGTGGGATTAATAAACATTCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAAATGTTGCCGCATCTTCTCAAGAAACACAGGCTGATTCTGGCATAATTGACAGCCATAGAGATATTGAATCTCATGGTCTACA
AACACAACTTAACAAAGATAATTTTGCTGACATTACCCCTTCAGACGAGATTGGCAGTCTGACAGAGATGAGGGATACAAGACAAGGTGGGATTAATGAACATCCAAGGAACATGGTAATGCCAGACAATGATGCTGGACAGTACACAGCTGTTGCAGTTTCTTCTCAAGAAACACAGGCTGATTCTGGACAGACAAAAT
TGCAGCCTATTTCAAATCCAATATTGGGAGAAAGAAATGAGGACTGTGCAGAGAAATGTGCAACAGAGTCTACAAAGCAGGATGCTCTATTGTCAGAAATGCAGGGACCAACAGCACCCTCACTGAACAAGAACCACGATGAAAACACAGGAAACTATGCTGCTGGGAAGGATATTGTATTGGCTAACTGGCAAGGAGGG
CCAGGATCATTGGTGGATGAACACATTCAGAATAATGGTTACGAGGATTCTTCACAGGCTTCTCAGAAGTCATATGCTACAGTAGAAGCACAAGGGACCTCAGAATCACCATTGGATAAAGGGACCGAGATTTCAGGGGTTCTACATGCAGATGTTCCACCAGGGGACTTTCAGGGAATGCCAGAATTTGTATCAGCTAA
AATTAAAGTTGAAAAGACAGAGCAATATTCTGCAAACACTTTGATGGAGGTTGATGTTCAGGGAATGCCAGAAGTTTTATCAGATAAAATCAGTGATGAAATGATGCAAAAGCATGCTGCGGACACTTTGATGGAAGTTGATGTTCAGGGAACGGGAGAAATTGTTTCAGATGAAGTCAGTTACGAAAAGACAGAACAGC
CTGCCAGAGACACTTTGATGGAGGTTGATGTTCAGGGCATGACAGAATTTGTATCAGAAAAAATCAATAATGAGAAGACTGAACAGCATAATGTAGACACTTCAATTGAGCTTGATGTGCAGGGTATGCCAGGATTTGTATCAGATAAAATCAATGATGAAAAGACAAATGAACATACTGTAGACACTTTGATGGAGGTT
GATATGCTGGACAAAATCCAAGACATGCCAGATCCATTGCAGGGTATGGAAAACAAAAAGGTTGGCGATCATGCCAATATCAAAACAGTTAATCAACAGGATAGTGGGCAACTAGAACAAATATCAGATTCGACGAGGGGTGAAATCAGTCAGGATAGTTCAGAGAAGCATGCCAGAGATTTTTCTTGCAAGGATATTAA
GTTAATGGAAATGCAAGTAATTCCGGATTCCTCATTGGAGGGAAGTAATGAGCATGGTGGAGGGACACATGTCATTGGGACCTCACCCAAGGTTCTTTCACAGGCTTGTAATGATGGGCAAGCAAAGTCTGGAGATGAGGAAAGCATGCTGGGCAGCGATAGACACCTACTGGAAACTAGTGTGCCTTTAGCAGAAAAGA
CCCAGGAACAGAAAGAAAATGGCCTGAAGACTGAAGAAAAGAGTTCAGCTGAGAAAATACTTACAACCGGTACCGTTGGATTCACTACTATAGATGCTGAGTCAGACAGTCAGGTTAAAGATTCAGAGGGGGTGATGATCAGTGTAGGGCTTGATTCCTCATCTATACCAGCTCCTTATGCACCGTCTAGTCTTCTTGAT
CATTCTTCATCTTATTTAAAGTTAACCAATGAAAAGGTGAGCACTCCAGTGATGCAGCATAAAAATGCTGAAACTCAAGATATTGAATCCCCCAGCCCTGTTTCCTTTCCGGATGATCAAAATCAGAATGCTTCATTGGCAAATCCTGCAGAGAAAGTTGTCCATGATGTCTTAGGCAGCTTAGATGAAAACAGTTCCAA
GAAAGCTAGTATTGATGTTACCCAAAATGAATCTGTGGATGTGCAAGGATTTGGGAATCGAGAGTCTCGTGCTCTTGAAAAACTATCTACTGTTGAGGCTTCTCTGGACGAAGAAAATGAAAGAAAAGATACATCAATAAGATCTGTTTTACCAACAAAAGACATTGAAAACGTGGTCAAAGCTACTTCTTCCCCAGAAG
GAAAAGATCTTCTTCTTTCTCATACCGGGGATGAAACACAAGGATGTAAGTTTGGGGCCATTTCCCTTCAAGTCCTTAATCAATCTGCAGTGCCAGCAGAGCAATCTATAACTGGCACATCACAGCAAGCATTGCAAGGAAACGATCAGTGTATTACATCAGAGTCTGAATATAGCAAGCACACTGATCAGATGAATCTT
GAGTTTGAAAGGAATGTTAAAGGAGATAATACCTTGGTTAGTATCAAAGTGCAGGATGAAAGGGAGACTGACACCACATTTTCTTCAGATGGGCAGCAGTCTATGCCAATACCCGACTCAGTTCATAGCACTCACCAAACTACAGTGAAAGTTTTACAACCAGATATTCCTGAAAGAAGTGTTATTTTGGGAGCACAAGA
AGATGAGCAAGAGGTTCATAATGTGATTATAGAATTGCCTGAGAATGAAACAGAAGAGCCTGGTAAATATAAAGGCCAGGCCGAGGCAACTGAGGTCAGTTTAGAAGTTGGGATCTCATTAGAACCTGATAATAGTTCTCCTGCAACAGATGGAGGCGGTGATCATTTGCAGAGTTGTTTGCTGCTTCCAAATGTGGATC
GGTCTTCTGCTGAGAATAGGGATCTTCTTCAGTCTGTGAAATTGTCTTCAGTTTCCAGTGAGGGGGATGATAAAGTGGAAAAGCTTGGGACTGAATCTGGAGATTTAGAAGCCAAGTCTGTGTCTCAAATTGGTGATACTTTCACATTTGAGATTGCAACAAAGAATGTTCCTGCTGAAGAGGGTATCTATGGCTCAGTA
GATTTTACCCCGTCTGGTGATATAGACAAAAATGTTGGATGCTGGAAACCCTTTCAAACTGGCCAACCAAGTGAAGTGATTCAGAACAAGGCTTCCGCACCATGCCAAACTGAGAAAGGTGAAGCTGTCTCTTGTCAAGAACATGCAAATGATAATGTTAAAGTTGCAGTCCCTGATACAATTTCAGTACTCCAGGAAGA
CAGTTCTCACTTTGCTGAAGCTAAAGCTAAACCTGTGCCTAGTGGAGAGAAAATAAGTCGTGCAACGGTGTCGGGCAAGAAGTCTACAAGTCATGACAAGAAATCTGGTGATAGTCAAGCGGCAAGTTTATGCACTATGGCCACTATCTGCAGTCCATCAGTCCGGCCAGGGAGCCATTCATCTAAAAGTGGGGCTAGAC
AGGAACCAAAGCTTTCACGTACAGATCTTCCTGATATCAACCCACAATTTACATCATCATGTACCATGCAGTTATCAACATTACCAGATTTGAATGCAACAGTAGTGCCTGCATCAGTGCTGTTTCAACAACCTTTCACAGATTCACAGCAAGTACAATTGCGTGCACAGATTCTTGTGTACGGGTCTCTTATTCAAGGC
TCATTACCAGATGAGGCGCTGATGATAACAGCTTTTTCTGATATAGGAGGAGCTCAAAATGGCACTTCGGCATCAGATGGAGGCAGGAGTATGTGGGAGAATGCTTGGCATATTGCTGCAGATCGTATTCATGGCAAGGGGTTCTCTAATACCTCGGACATAACTGCTCATACTCCTTGCTCGAGCACAACAGGAACAGT
ATCTAGCAAAATTGTTGAAGTCACGAAGATTCCAACACCTTCTGCATCAATTAATGCTACTGATGTGAGGAATCCTGTTTCAGGAATGCGGTCAGCAGATTTGGCAGTGTCAAGGAGCCATTTATTGCCAGCTAGAATGACTGGGGCTTCTAACATAGGGAGGGCTGGGAGCAAAAATACCCCCCGGGCAATTATGAGTC
CACCTCGCCCACTCACACTGTCACCACCAGTGTGGAGTACTCCAGTGAATGAAGGGGTGCAGGGATCCACCATTCAGAGGAGTCTGCATCTCGACCCCCATCAAATGACTGCAGCAAGGCATCTCTATCAATCTGCACAGCTTGGACACTACCCCACACCAGGAAGTGCTACACCTTGGATGCCTCACATGCCTTTCCCT
GGGCCATGGGTGGCCCCTCCTCAAGGACCGCCACCAGAGCCTGGCTTGCAGATTTCTAATTTTGCGGTTCCAGAAGTAACTCAGGATGCACTCAATAACCGGAGGAATTCAGTTTCATCTTCAGTACCATATACGCCAATGACACCTGCTGTTCCTCATTTCCAAAGTATGGTGGGTGCTACTACAGGTCACACTAGTAC
CAATGTTGGTACAGATGTTCCAAAAGCAGGTGCGGCTGTTGGTAGGCAGTCCAGCAAGGAGCAGAAGACTAGAAAGAGAAAGAAGAAAAGTGTTTCAGAAGAAATTGTTCAAACTGGTGGCATACAAGTGACACCCGGTGTTTCTAAACACTCTGCTGAACACACACCAATAGCTTCCCCTGGGTCGTCAATGATTGTCT
CAACTACTTCAAATGCTCTCATTGCACCAGGTACTGGCATCCCGGTTTTACCTTCTGTTTCTCATTACCAGGTAATAAGTAGAGGAGAAACTGGACAGAGAATTATATTTTCAGAGGAAACAGCAACTCGTATAGAGCAGGCCAGATTGAATGCTGAAGAAGCTGCTTCTATTGCAGCTACTGCAGTTAGGCACAGCCAG
GGCATTTGGAGTCAGTTAGCATCTCAAAAGAATTCTGGATTAGTTTCTGACTGTGAAGCTAAGCTTGCTTCTGCGGCTGTGGCAGCAGCGGCAGCTGCATCGGTTGCTAAGGCTGCAGCAGCAGCAGCCCAGATTGCATCTGATGCTGCTCTACAAGCAAAGCTCATGGCAACTGAGGCTCTGAGTTCTGATGGTAAACA
AGATGCTATTTATTCTAGTGAAGTTGGATTGCTTGATGGCAGCCAGGGCCATTCTCATTCAATTATTGCAACTGCCAGGGAAGCTGCCAAGAAGAGGGTAGAGGCTGCTTCAGCTGCTACAAAGAGGGCAGAGAATTTGGATGCAGTTGTGAGGGCAGCTGAACTAGCTGCACAAGCTGTATCACAAGCAGGAGCTGTTG
TGGCCATGGGTGATCCTGTTCCATTACCACTAAATGTTTTATTGGAAGCTGGTCCAGAGGCTTATTGGAAGCTAATGACCCAAGAAGCAAGTATTCGAAAAGATGATAATGTTCATAATGAGACACCATCTCAACAATTTGTTACCAGAAATGAAAATATTGTTAAATCTTATAAATTCAAAGATAAGAGAGATGTGTTC
CGGAATGAAACCTCAAGGAATACAAAAGAAGGCACGAAGGGGCCTGCAACAGAATTGTCAAAGCAGTCAGAGAAGGATTGTTCACAGACACCAGATCGTGCACTGCATGATATCTTAAGTGAAAAAGATGTGGAAGCTCGAAAGGAGAGTCAAGCACTTCGTACAACCCAAGTTGCTGTAGCTGTCTCTGTTGCAGAGAC
CATCCCAAGGGGGGAAATAGTAGCTCTTGAAACTGAACCTAGCTCTAAAAATCATAAGGAGCCATTTGCTTCAAAAGTTAATGACATCAAGGAGGGATCGCTTGTAGAGGTTGTCTCTGATGAAGAGGGTCTTCGGGGAGTTTGGTTTTCAGCCAAAGTACTGAACTTGAAAGATGGGAAAGCTTTTGTCCGTTATGATG
AACTACTTTCAGATGAAGgtcgtctaaatcatcaaacttagtatcttgcatctttctctgttttacaaaactgtttatgaattctttattaagtggaagccattgatgtaacaaagcatgcatgatatgatttatggcacgtccttcaaataatgcagGCCCAGGTCAGCTTCAAGAGTGGATACCTTTGAAGGGAGATA
ATGGAAACGCGCCTAGGATTCGTATGGCTCATCCTATGACTGTAATCAAATTTGAAGGCACACGGAAGCGCCGCAGAGCAGCAATGGGTAATTATATTTGGTCTGTGGGAGATCATGTGGATGCATGGATTCGTGATGGGTGGTGGGAAGGCATTGTTAAGGGCATAAAGGACAATGATGAAAGCAAACTTATTGTTCAT
TTTCCAGGAGAAGGTGATACATCTATAATCAAAGCTTGGAACTTACGACCATCATTGATTTGGAAAGATGGTCAATGGGTCGAATGGACAAGTTTAAAGGCAGAGAAGAATGTAGCACACGGGGATGAACTTCCTCCTGAAAAACGACAAAAGCTGGATAATCATGAAACTGAAGGAGATGCAAAAGGCAAAGAGAAGTT
GCAAAAGTTTCCCAACATAGAGGAAGCTAAGAAAATGCAAAAGATTCCTATCAATGAGGAAGTTAAGAAAATGCAAAAGATTCCCAATATAGAGGAAGCCAAGAAAATGCACGAGCTGAATGCTGTAATGTCATTTAGTAAAGATAAGTTTCTCACAGCTGCCAAGAGCTTGACAGATGATGGCAACTCAGCAAGCATTA
AAGTTAACCAAATAGGGCTTCAGACAGAAGGTTCCAAGGTGGTTTTTGGTGTTCCCAGGCAAGCAAAGAAAAGAAAATTTATGGATGTAAGCAAGCATTATGTTGCTGATACGGTTGTAAGGAACTTCCCTGATGGAAAATCTTCTGGTAAAGTTGATATTCCCAAATCATCAGTATTTAACAGCCATGGATGGAAAACT
GCCAATAAGGCAGAAGATAAAAAGTCAAAGAAGCCAATGATTTCTAGACCTAAAGATCTAAAATTGGTTAAAGATCAGGATGGACAAAGCAAGGCAAGGTCTGAAAAGGATAAAAGTTCTTTTGCCAATCCGACAAAAGACATATCAGGTCAGGATTTCAGTTTGTCTGCCATACCTAAGCCAGGCAGTAGAAGGTTTGG
GCTAGGGGGAAATGTTCCTGCCTCTCATAAATCAGGTGAAGCCCCACATTTATCTGTTTCAAAGGAAACCATGATACGTAGGCCTTCACCTTCTACCAAAGTGAAAACTTCTGCAACAGAGTCAGAACAGGGATCAAAAGGAAAGCAGTCATCGGCGTCAGATAGCTCAAGTCTTAACGTAGAAAAGGGCATACTTCCTC
AGGAGGTGACAAAAGATAAAGTTGCAGGAAAATCAACAGCTGATATAATGGAGCCACGTAGATCAAATCGCAGAATCCAGCCGACTTCAAGGTTGTTGGAAGGACTACAAACGTCACCCACTGTTGGGAAGAGTTCTTCAACTAATCTTTCACTTGAAAAGGGAGGAAAAGCTCAGTCGAAGACATCATTTTCAAGAAAT
ACTAAAGACTGATGTAGGGCAGTGCCTACAAATTTCAGTCTATGCATGCATCATCATATGAATTTTGGTCCCCTATTCAAGAATGTAATTACGCAGTGTAAAGAGGGTGCACTACCATTGATTTCTGACAAAGCATAAAGACAGAAATCAATCTGGAAGCACTGTCCTCCATCAAAATCGTACATGTAATATATGATTTC
ATAATTCTTAATACCTTCTCTCCATCTGTGTCAAATGATTTGAAAATGCTGGAGCAG